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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AciI | CCGC | 2 cut(s) | 316, 372 |
| AclWI | GGATC | 1 cut(s) | 369 |
| AcoI | YGGCCR | 1 cut(s) | 157 |
| AcsI | RAATTY | 1 cut(s) | 241 |
| AfiI | CCNNNNNNNGG | 3 cut(s) | 213, 214, 215 |
| AflII | CTTAAG | 2 cut(s) | 269, 395 |
| AgsI | TTSAA | 2 cut(s) | 15, 227 |
| AhlI | ACTAGT | 1 cut(s) | 152 |
| AluBI | AGCT | 3 cut(s) | 72, 343, 400 |
| AluI | AGCT | 3 cut(s) | 72, 343, 400 |
| Alw26I | GTCTC | 1 cut(s) | 304 |
| AlwI | GGATC | 1 cut(s) | 369 |
| AoxI | GGCC | 2 cut(s) | 157, 265 |
| ApeKI | GCWGC | 1 cut(s) | 340 |
| ApoI | RAATTY | 1 cut(s) | 241 |
| AspA2I | CCTAGG | 1 cut(s) | 212 |
| AspS9I | GGNCC | 1 cut(s) | 386 |
| AsuHPI | GGTGA | 2 cut(s) | 143, 269 |
| AvaII | GGWCC | 1 cut(s) | 386 |
| AvrII | CCTAGG | 1 cut(s) | 212 |
| BaeGI | GKGCMC | 1 cut(s) | 356 |
| BalI | TGGCCA | 1 cut(s) | 159 |
| BbsI | GAAGAC | 1 cut(s) | 183 |
| BbvI | GCAGC | 1 cut(s) | 352 |
| BccI | CCATC | 3 cut(s) | 19, 194, 244 |
| BcoDI | GTCTC | 1 cut(s) | 304 |
| BcuI | ACTAGT | 1 cut(s) | 152 |
| BfaI | CTAG | 3 cut(s) | 153, 213, 344 |
| BfrI | CTTAAG | 2 cut(s) | 269, 395 |
| BisI | GCNGC | 1 cut(s) | 341 |
| BlnI | CCTAGG | 1 cut(s) | 212 |
| BlsI | GCNGC | 1 cut(s) | 342 |
| Bme18I | GGWCC | 1 cut(s) | 386 |
| BmgT120I | GGNCC | 1 cut(s) | 386 |
| BmiI | GGNNCC | 2 cut(s) | 205, 387 |
| BpiI | GAAGAC | 1 cut(s) | 183 |
| BpuEI | CTTGAG | 1 cut(s) | 52 |
| BsaJI | CCNNGG | 2 cut(s) | 75, 212 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 56, 86 |
| Bsc4I | CCNNNNNNNGG | 3 cut(s) | 213, 214, 215 |
| Bse1I | ACTGG | 1 cut(s) | 196 |
| BseDI | CCNNGG | 2 cut(s) | 75, 212 |
| BseGI | GGATG | 1 cut(s) | 67 |
| BseLI | CCNNNNNNNGG | 3 cut(s) | 213, 214, 215 |
| BseMII | CTCAG | 1 cut(s) | 162 |
| BseNI | ACTGG | 1 cut(s) | 196 |
| BseRI | GAGGAG | 3 cut(s) | 64, 116, 381 |
| BseSI | GKGCMC | 1 cut(s) | 356 |
| BseXI | GCAGC | 1 cut(s) | 352 |
| BshFI | GGCC | 2 cut(s) | 159, 267 |
| BslI | CCNNNNNNNGG | 3 cut(s) | 213, 214, 215 |
| BsmAI | GTCTC | 1 cut(s) | 304 |
| BsnI | GGCC | 2 cut(s) | 159, 267 |
| Bsp1286I | GDGCHC | 1 cut(s) | 356 |
| Bsp143I | GATC | 1 cut(s) | 361 |
| BspACI | CCGC | 2 cut(s) | 316, 372 |
| BspANI | GGCC | 2 cut(s) | 159, 267 |
| BspCNI | CTCAG | 1 cut(s) | 163 |
| BspLI | GGNNCC | 2 cut(s) | 205, 387 |
| BspPI | GGATC | 1 cut(s) | 369 |
| BspTI | CTTAAG | 2 cut(s) | 269, 395 |
| BsrI | ACTGG | 1 cut(s) | 196 |
| BssECI | CCNNGG | 2 cut(s) | 75, 212 |
| BssMI | GATC | 1 cut(s) | 361 |
| BssT1I | CCWWGG | 1 cut(s) | 212 |
| Bst4CI | ACNGT | 1 cut(s) | 358 |
| Bst6I | CTCTTC | 1 cut(s) | 342 |
| BstAFI | CTTAAG | 2 cut(s) | 269, 395 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 38 |
| BstC8I | GCNNGC | 1 cut(s) | 234 |
| BstDEI | CTNAG | 1 cut(s) | 171 |
| BstF5I | GGATG | 1 cut(s) | 67 |
| BstKTI | GATC | 1 cut(s) | 364 |
| BstMAI | GTCTC | 1 cut(s) | 304 |
| BstMBI | GATC | 1 cut(s) | 361 |
| BstMWI | GCNNNNNNNGC | 1 cut(s) | 38 |
| BstSLI | GKGCMC | 1 cut(s) | 356 |
| BstV1I | GCAGC | 1 cut(s) | 352 |
| BstV2I | GAAGAC | 1 cut(s) | 183 |
| BsuRI | GGCC | 2 cut(s) | 159, 267 |
| BtsCI | GGATG | 1 cut(s) | 67 |
| BtsI | GCAGTG | 1 cut(s) | 243 |
| BtsIMutI | CAGTG | 3 cut(s) | 189, 243, 354 |
| Cac8I | GCNNGC | 1 cut(s) | 234 |
| Cfr13I | GGNCC | 1 cut(s) | 386 |
| CspCI | CAANNNNNGTGG | 2 cut(s) | 178, 213 |
| CviAII | CATG | 1 cut(s) | 161 |
| CviJI | RGCY | 5 cut(s) | 72, 159, 267, 343, 400 |
| CviKI_1 | RGCY | 5 cut(s) | 72, 159, 267, 343, 400 |
| DdeI | CTNAG | 1 cut(s) | 171 |
| DpnI | GATC | 1 cut(s) | 363 |
| DpnII | GATC | 1 cut(s) | 361 |
| EaeI | YGGCCR | 1 cut(s) | 157 |
| Eam1104I | CTCTTC | 1 cut(s) | 342 |
| EarI | CTCTTC | 1 cut(s) | 342 |
| Eco130I | CCWWGG | 1 cut(s) | 212 |
| Eco147I | AGGCCT | 1 cut(s) | 267 |
| Eco47I | GGWCC | 1 cut(s) | 386 |
| EcoO109I | RGGNCCY | 1 cut(s) | 386 |
| EcoRI | GAATTC | 1 cut(s) | 241 |
| EcoT14I | CCWWGG | 1 cut(s) | 212 |
| ErhI | CCWWGG | 1 cut(s) | 212 |
| FaeI | CATG | 1 cut(s) | 164 |
| FaiI | YATR | 4 cut(s) | 162, 248, 278, 409 |
| FatI | CATG | 1 cut(s) | 160 |
| FauI | CCCGC | 1 cut(s) | 379 |
| Fnu4HI | GCNGC | 1 cut(s) | 341 |
| FokI | GGATG | 1 cut(s) | 74 |
| Fsp4HI | GCNGC | 1 cut(s) | 341 |
| FspBI | CTAG | 3 cut(s) | 153, 213, 344 |
| GluI | GCNGC | 1 cut(s) | 341 |
| HaeIII | GGCC | 2 cut(s) | 159, 267 |
| Hin1II | CATG | 1 cut(s) | 164 |
| HincII | GTYRAC | 2 cut(s) | 6, 138 |
| HindII | GTYRAC | 2 cut(s) | 6, 138 |
| HindIII | AAGCTT | 1 cut(s) | 398 |
| HinfI | GANTC | 3 cut(s) | 65, 80, 312 |
| HphI | GGTGA | 2 cut(s) | 143, 269 |
| Hpy166II | GTNNAC | 2 cut(s) | 6, 138 |
| Hpy188I | TCNGA | 1 cut(s) | 79 |
| Hpy188III | TCNNGA | 1 cut(s) | 116 |
| Hpy8I | GTNNAC | 2 cut(s) | 6, 138 |
| HpyAV | CCTTC | 1 cut(s) | 52 |
| HpyCH4III | ACNGT | 1 cut(s) | 358 |
| HpyCH4V | TGCA | 4 cut(s) | 41, 125, 186, 232 |
| HpyF10VI | GCNNNNNNNGC | 1 cut(s) | 38 |
| HpyF3I | CTNAG | 1 cut(s) | 171 |
| Hsp92II | CATG | 1 cut(s) | 164 |
| Kzo9I | GATC | 1 cut(s) | 361 |
| LmnI | GCTCC | 2 cut(s) | 77, 103 |
| LpnPI | CCDG | 3 cut(s) | 101, 177, 249 |
| Lsp1109I | GCAGC | 1 cut(s) | 352 |
| MaeI | CTAG | 3 cut(s) | 153, 213, 344 |
| MaeIII | GTNAC | 1 cut(s) | 365 |
| MalI | GATC | 1 cut(s) | 363 |
| MboI | GATC | 1 cut(s) | 361 |
| MboII | GAAGA | 2 cut(s) | 188, 359 |
| MhlI | GDGCHC | 1 cut(s) | 356 |
| MlsI | TGGCCA | 1 cut(s) | 159 |
| MluCI | AATT | 3 cut(s) | 221, 241, 403 |
| MluNI | TGGCCA | 1 cut(s) | 159 |
| MlyI | GAGTC | 2 cut(s) | 59, 306 |
| MnlI | CCTC | 8 cut(s) | 85, 94, 166, 219, 325, 343, 399, 402 |
| Mox20I | TGGCCA | 1 cut(s) | 159 |
| MscI | TGGCCA | 1 cut(s) | 159 |
| MseI | TTAA | 4 cut(s) | 167, 270, 396, 402 |
| Msp20I | TGGCCA | 1 cut(s) | 159 |
| MspCI | CTTAAG | 2 cut(s) | 269, 395 |
| MwoI | GCNNNNNNNGC | 1 cut(s) | 38 |
| NdeII | GATC | 1 cut(s) | 361 |
| NlaIII | CATG | 1 cut(s) | 164 |
| NlaIV | GGNNCC | 2 cut(s) | 205, 387 |
| PceI | AGGCCT | 1 cut(s) | 267 |
| PfeI | GAWTC | 1 cut(s) | 80 |
| PkrI | GCNGC | 1 cut(s) | 342 |
| PleI | GAGTC | 2 cut(s) | 59, 306 |
| PpsI | GAGTC | 2 cut(s) | 59, 306 |
| PpuMI | RGGWCCY | 1 cut(s) | 386 |
| Psp5II | RGGWCCY | 1 cut(s) | 386 |
| PspN4I | GGNNCC | 2 cut(s) | 205, 387 |
| PspPI | GGNCC | 1 cut(s) | 386 |
| PspPPI | RGGWCCY | 1 cut(s) | 386 |
| SaqAI | TTAA | 4 cut(s) | 167, 270, 396, 402 |
| SatI | GCNGC | 1 cut(s) | 341 |
| Sau3AI | GATC | 1 cut(s) | 361 |
| Sau96I | GGNCC | 1 cut(s) | 386 |
| SchI | GAGTC | 2 cut(s) | 59, 306 |
| SduI | GDGCHC | 1 cut(s) | 356 |
| SetI | ASST | 4 cut(s) | 74, 133, 345, 402 |
| SinI | GGWCC | 1 cut(s) | 386 |
| SmlI | CTYRAG | 3 cut(s) | 67, 269, 395 |
| SmoI | CTYRAG | 3 cut(s) | 67, 269, 395 |
| SpeI | ACTAGT | 1 cut(s) | 152 |
| Sse9I | AATT | 3 cut(s) | 221, 241, 403 |
| SseBI | AGGCCT | 1 cut(s) | 267 |
| SsiI | CCGC | 2 cut(s) | 316, 372 |
| SspI | AATATT | 1 cut(s) | 37 |
| SspMI | CTAG | 3 cut(s) | 153, 213, 344 |
| StuI | AGGCCT | 1 cut(s) | 267 |
| StyI | CCWWGG | 1 cut(s) | 212 |
| TaaI | ACNGT | 1 cut(s) | 358 |
| TaqI | TCGA | 1 cut(s) | 83 |
| TasI | AATT | 3 cut(s) | 221, 241, 403 |
| TfiI | GAWTC | 1 cut(s) | 80 |
| Tru1I | TTAA | 4 cut(s) | 167, 270, 396, 402 |
| Tru9I | TTAA | 4 cut(s) | 167, 270, 396, 402 |
| TscAI | CASTG | 3 cut(s) | 196, 243, 361 |
| TseI | GCWGC | 1 cut(s) | 340 |
| TspRI | CASTG | 3 cut(s) | 196, 243, 361 |
| Vha464I | CTTAAG | 2 cut(s) | 269, 395 |
| VpaK11BI | GGWCC | 1 cut(s) | 386 |
| XapI | RAATTY | 1 cut(s) | 241 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 188 |
| XmaJI | CCTAGG | 1 cut(s) | 212 |
| XspI | CTAG | 3 cut(s) | 153, 213, 344 |