FvH4_5g20080

Zeaxanthin epoxidase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0012148
Physical Location & Seq
Reverse (-)
11833756 .. 11837603
3848 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g20080.t1

Sequence Viewer

Length: 1359 bp
ATGGGAATAGTCTCTTTGTCCCTAGTTCTTCACAGCCGACTTGTTTCACCATACCATTCTCAAACTAGACCAATTCGTCTTAGTCAATCTAATTGGTTCCAAGCTCCAATCCCAGCAAGAACCAAACACACTTCTTTATCAATCATCAGAGCTCAATCTGGTGCCCGCAAGGAGGAAATTGTCATAGTTGGCGCTGGAATCGCCGGCCTTGCCACCGCTCTATCTCTTCACAGGCTTGGAATTGGATCATTGGTGGTAGAGCAAGCAGAGTCACTTCGAACCAGTGGAACTTCTCTCACCCTCTTCAAAAACGGTTGGAGGGTTTTGGATGCACTTGGAGTTGGAGACGATCTCAGAAACCAATTTCTTGAAGTTCAAGGGATGGTAGTAACAACAGCTGAGGGGAATGAGCTACGCTCCTTCAAGTTCAAAGAGGAAGATGAAAGCCAAGAGGTGCGTGCCGTAGAGAGGAGAGTACTACTTGAGACTCTTGCCAATCAACTGCCCCAAGGGGCGGTCCGTTTCTCTTCAAAGCTTGCAAAGATTGAGAAGATTGAAGATGGAGATACTTTGCTGCAACTTGTGGATGGTACTCAGCTATCTGCTAAGATTGTAATTGGCTGTGATGGGATTCGGTCTCCTATAGCTAAGTGGATGGGGTTTCCCGAGCCTAAGTATGCAGGGCATTGTGGTCTTCGTGGCCTTGCATTCTTTCCTGGTGGCCATAAGTTTGAACCAAAACTGAATCAAATCTATGGAAGAGGGCAGCGTGCTGGCATTGTTCCTATTTCTCCTACAAAAGTCTACTGGTTTGTCTGCTTCAACATGCCATCTCCAGGTCAAAAAATCACTGACCCAGCTGTGCTGAAGAAGCTAGCTAAAGAACTGGTAACAAACTGGCCTTCAGACCTATTGGACATCATAGACCATACCCCGGATGACACAATCAGTCGAACACCACTAGTAGACCGGTGGTTGTGGCCATCCATCAGCCCTCCGGCTTCAGCCGGAAGAGTTGTACTGGTTGGAGATGCATGGCATCCGATGACCCCCAATCTTGGACAAGGCGCTTGTTGTGCTTTGGAAGATTCAGTAATTTTGGCGCGAAAGCTTGCAGCAGCGATCGAGTCAGGACCAGCAGCCATTGAAGATGCTCTCAGCTCATACGGAAGTGAAAGATGGCCTCGAATCTTTCCGCTAACCATACGGGCAAACCTCATAGGGTCACTACTGCAATGGGAGAATCCTGTTGTGTGTTCTGTCCGGAACAATGTCATTATCCCAAAGCTTGTTAAGCTAGGACCAATGTTGGAACACACAAATTTTGACTGCCAACCCCTACAGAATCAAGTCGTGTAA

Protein Analysis

453

Amino Acids

49.46

Weight (kDa)

8.77

Isoelectric Point (pI)

41.19

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FAD_binding_3 PF01494 59 - 396 1.2e-28 FAD binding domain
Lycopene_cycl PF05834 60 - 213 3.5e-07 Lycopene cyclase protein
DAO PF01266 60 - 108 4.7e-06 FAD dependent oxidoreductase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012148)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G35660 AT2G35660 AT2G35660
fragaria_vesca FvH4_5g20080
malus_domestica MD06G1049200.v1.1
prunus_persica Prupe.5G059700_v2.0.a1 Prupe.5G059700_v2.0.a1
rosa_chinensis RchiOBHm_Chr7g0204941
rosa_laevigata RLG00000003420
rosa_multiflora Rmu_co8273435.1_g000001 Rmu_sc0002180.1_g000002
rosa_roxburghii Rroxscaffold_3G00252650
rosa_rugosa Rorug07G0086700 Rorug07G0086800
rosa_samantha Rh7AG217400 Rh7BG214300 Rh7CG230700 Rh7DG225000
rosa_wichuraiana Rw7G018860

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 75
AccB1I GGYRCC 1 cut(s) 161
AccBSI CCGCTC 1 cut(s) 218
AccI GTMKAC 2 cut(s) 804, 966
AccII CGCG 1 cut(s) 1105
AccIII TCCGGA 1 cut(s) 1263
AciI CCGC 4 cut(s) 166, 216, 515, 1196
AclWI GGATC 1 cut(s) 253
AcoI YGGCCR 2 cut(s) 721, 980
AcsI RAATTY 1 cut(s) 1321
AcuI CTGAAG 3 cut(s) 887, 888, 987
AfaI GTAC 3 cut(s) 477, 592, 1020
AfiI CCNNNNNNNGG 6 cut(s) 172, 468, 514, 836, 934, 1058
AgeI ACCGGT 1 cut(s) 969
AhlI ACTAGT 1 cut(s) 961
AjnI CCWGG 2 cut(s) 715, 835
Alw21I GWGCWC 1 cut(s) 154
Alw26I GTCTC 4 cut(s) 16, 339, 479, 642
AlwI GGATC 1 cut(s) 253
Ama87I CYCGRG 1 cut(s) 665
Aor13HI TCCGGA 1 cut(s) 1263
AoxI GGCC 6 cut(s) 205, 700, 721, 899, 980, 1181
ApeKI GCWGC 5 cut(s) 574, 766, 1115, 1118, 1139
ApoI RAATTY 1 cut(s) 1321
AsiGI ACCGGT 1 cut(s) 969
AspLEI GCGC 3 cut(s) 194, 1070, 1105
AspS9I GGNCC 3 cut(s) 517, 1133, 1301
AsuC2I CCSGG 1 cut(s) 935
AsuHPI GGTGA 2 cut(s) 39, 289
AsuII TTCGAA 1 cut(s) 277
AsuNHI GCTAGC 1 cut(s) 874
AvaI CYCGRG 1 cut(s) 665
AvaII GGWCC 3 cut(s) 517, 1133, 1301
BaeGI GKGCMC 1 cut(s) 166
BalI TGGCCA 2 cut(s) 723, 982
BanI GGYRCC 1 cut(s) 161
BanII GRGCYC 1 cut(s) 154
BbsI GAAGAC 1 cut(s) 686
Bbv12I GWGCWC 1 cut(s) 154
BbvCI CCTCAGC 1 cut(s) 399
BbvI GCAGC 5 cut(s) 561, 778, 1127, 1130, 1151
BccI CCATC 9 cut(s) 376, 554, 581, 620, 649, 838, 991, 995, 1173
BceAI ACGGC 1 cut(s) 446
BciT130I CCWGG 2 cut(s) 717, 837
BcnI CCSGG 1 cut(s) 935
BcoDI GTCTC 4 cut(s) 16, 339, 479, 642
BcuI ACTAGT 1 cut(s) 961
BfaI CTAG 5 cut(s) 23, 66, 875, 962, 1298
BfmI CTRYAG 2 cut(s) 642, 1340
BfoI RGCGCY 2 cut(s) 195, 1071
BisI GCNGC 5 cut(s) 575, 767, 1116, 1119, 1140
BlsI GCNGC 5 cut(s) 576, 768, 1117, 1120, 1141
BmcAI AGTACT 1 cut(s) 477
Bme1390I CCNGG 3 cut(s) 717, 837, 935
Bme18I GGWCC 3 cut(s) 517, 1133, 1301
BmeT110I CYCGRG 1 cut(s) 665
BmgT120I GGNCC 3 cut(s) 517, 1133, 1301
BmiI GGNNCC 2 cut(s) 98, 163
BmrFI CCNGG 3 cut(s) 717, 837, 935
BmsI GCATC 4 cut(s) 319, 1021, 1048, 1141
BmtI GCTAGC 1 cut(s) 878
BpiI GAAGAC 1 cut(s) 686
BplI GAGNNNNNCTC 4 cut(s) 336, 368, 401, 433
BpmI CTGGAG 1 cut(s) 819
Bpu10I CCTNAGC 1 cut(s) 399
Bpu14I TTCGAA 1 cut(s) 277
BpuEI CTTGAG 1 cut(s) 503
BpuMI CCSGG 1 cut(s) 935
BsaI GGTCTC 1 cut(s) 642
BsaJI CCNNGG 2 cut(s) 508, 933
BsaWI WCCGGW 2 cut(s) 969, 1263
Bsc4I CCNNNNNNNGG 6 cut(s) 172, 468, 514, 836, 934, 1058
Bse118I RCCGGY 2 cut(s) 203, 969
Bse1I ACTGG 5 cut(s) 282, 812, 891, 902, 1026
Bse3DI GCAATG 1 cut(s) 1241
BseAI TCCGGA 1 cut(s) 1263
BseBI CCWGG 2 cut(s) 717, 837
BseDI CCNNGG 2 cut(s) 508, 933
BseGI GGATG 7 cut(s) 334, 387, 592, 660, 943, 983, 1039
BseLI CCNNNNNNNGG 6 cut(s) 172, 468, 514, 836, 934, 1058
BseMI GCAATG 1 cut(s) 1241
BseMII CTCAG 4 cut(s) 367, 390, 608, 1171
BseNI ACTGG 5 cut(s) 282, 812, 891, 902, 1026
BseRI GAGGAG 1 cut(s) 484
BseSI GKGCMC 1 cut(s) 166
BseXI GCAGC 5 cut(s) 561, 778, 1127, 1130, 1151
BseYI CCCAGC 2 cut(s) 112, 856
Bsh1236I CGCG 1 cut(s) 1105
Bsh1285I CGRYCG 1 cut(s) 1125
BshFI GGCC 6 cut(s) 207, 702, 723, 901, 982, 1183
BshNI GGYRCC 1 cut(s) 161
BshTI ACCGGT 1 cut(s) 969
BsiEI CGRYCG 1 cut(s) 1125
BsiHKAI GWGCWC 1 cut(s) 154
BsiHKCI CYCGRG 1 cut(s) 665
BsiSI CCGG 6 cut(s) 204, 935, 970, 998, 1008, 1264
BslFI GGGAC 1 cut(s) 4
BslI CCNNNNNNNGG 6 cut(s) 172, 468, 514, 836, 934, 1058
BsmAI GTCTC 4 cut(s) 16, 339, 479, 642
BsmBI CGTCTC 1 cut(s) 339
BsmFI GGGAC 1 cut(s) 4
BsmI GAATGC 1 cut(s) 707
BsnI GGCC 6 cut(s) 207, 702, 723, 901, 982, 1183
Bso31I GGTCTC 1 cut(s) 642
BsoBI CYCGRG 1 cut(s) 665
Bsp119I TTCGAA 1 cut(s) 277
Bsp1286I GDGCHC 2 cut(s) 154, 166
Bsp13I TCCGGA 1 cut(s) 1263
Bsp143I GATC 3 cut(s) 245, 349, 1122
BspACI CCGC 4 cut(s) 166, 216, 515, 1196
BspANI GGCC 6 cut(s) 207, 702, 723, 901, 982, 1183
BspCNI CTCAG 4 cut(s) 366, 391, 607, 1170
BspEI TCCGGA 1 cut(s) 1263
BspFNI CGCG 1 cut(s) 1105
BspLI GGNNCC 2 cut(s) 98, 163
BspOI GCTAGC 1 cut(s) 878
BspPI GGATC 1 cut(s) 253
BspT104I TTCGAA 1 cut(s) 277
BspT107I GGYRCC 1 cut(s) 161
BspTNI GGTCTC 1 cut(s) 642
BsrBI CCGCTC 1 cut(s) 218
BsrDI GCAATG 1 cut(s) 1241
BsrFI RCCGGY 2 cut(s) 203, 969
BsrI ACTGG 5 cut(s) 282, 812, 891, 902, 1026
BssAI RCCGGY 2 cut(s) 203, 969
BssECI CCNNGG 2 cut(s) 508, 933
BssMI GATC 3 cut(s) 245, 349, 1122
BssT1I CCWWGG 1 cut(s) 508
Bst2UI CCWGG 2 cut(s) 717, 837
Bst4CI ACNGT 1 cut(s) 314
Bst6I CTCTTC 5 cut(s) 231, 308, 532, 754, 1006
BstBI TTCGAA 1 cut(s) 277
BstC8I GCNNGC 9 cut(s) 166, 205, 264, 459, 537, 771, 775, 876, 1113
BstDEI CTNAG 8 cut(s) 80, 353, 399, 594, 606, 648, 672, 1157
BstF5I GGATG 7 cut(s) 334, 387, 592, 660, 943, 983, 1039
BstFNI CGCG 1 cut(s) 1105
BstH2I RGCGCY 2 cut(s) 195, 1071
BstHHI GCGC 3 cut(s) 194, 1070, 1105
BstKTI GATC 3 cut(s) 248, 352, 1125
BstMAI GTCTC 4 cut(s) 16, 339, 479, 642
BstMBI GATC 3 cut(s) 245, 349, 1122
BstMCI CGRYCG 1 cut(s) 1125
BstMWI GCNNNNNNNGC 5 cut(s) 200, 209, 871, 1076, 1294
BstNI CCWGG 2 cut(s) 717, 837
BstNSI RCATGY 1 cut(s) 829
BstSCI CCNGG 3 cut(s) 715, 835, 933
BstSFI CTRYAG 2 cut(s) 642, 1340
BstSLI GKGCMC 1 cut(s) 166
BstUI CGCG 1 cut(s) 1105
BstV1I GCAGC 5 cut(s) 561, 778, 1127, 1130, 1151
BstV2I GAAGAC 1 cut(s) 686
BsuRI GGCC 6 cut(s) 207, 702, 723, 901, 982, 1183
BtsCI GGATG 7 cut(s) 334, 387, 592, 660, 943, 983, 1039
BtsIMutI CAGTG 2 cut(s) 289, 849
Cac8I GCNNGC 9 cut(s) 166, 205, 264, 459, 537, 771, 775, 876, 1113
CfoI GCGC 3 cut(s) 194, 1070, 1105
Cfr10I RCCGGY 2 cut(s) 203, 969
Cfr13I GGNCC 3 cut(s) 517, 1133, 1301
CpoI CGGWCCG 1 cut(s) 517
Csp6I GTAC 3 cut(s) 476, 591, 1019
CspAI ACCGGT 1 cut(s) 969
CspI CGGWCCG 1 cut(s) 517
CviAII CATG 2 cut(s) 826, 1035
CviQI GTAC 3 cut(s) 476, 591, 1019
DdeI CTNAG 8 cut(s) 80, 353, 399, 594, 606, 648, 672, 1157
DpnI GATC 3 cut(s) 247, 351, 1124
DpnII GATC 3 cut(s) 245, 349, 1122
DrdI GACNNNNNNGTC 1 cut(s) 75
DseDI GACNNNNNNGTC 1 cut(s) 75
EaeI YGGCCR 2 cut(s) 721, 980
Eam1104I CTCTTC 5 cut(s) 231, 308, 532, 754, 1006
EarI CTCTTC 5 cut(s) 231, 308, 532, 754, 1006
Ecl136II GAGCTC 1 cut(s) 152
Eco130I CCWWGG 1 cut(s) 508
Eco24I GRGCYC 1 cut(s) 154
Eco31I GGTCTC 1 cut(s) 642
Eco47I GGWCC 3 cut(s) 517, 1133, 1301
Eco53kI GAGCTC 1 cut(s) 152
Eco57I CTGAAG 3 cut(s) 887, 888, 987
Eco88I CYCGRG 1 cut(s) 665
EcoICRI GAGCTC 1 cut(s) 152
EcoRII CCWGG 2 cut(s) 715, 835
EcoT14I CCWWGG 1 cut(s) 508
EcoT22I ATGCAT 1 cut(s) 1036
EcoT38I GRGCYC 1 cut(s) 154
ErhI CCWWGG 1 cut(s) 508
Esp3I CGTCTC 1 cut(s) 339
FaeI CATG 2 cut(s) 829, 1038
FaqI GGGAC 1 cut(s) 4
FatI CATG 2 cut(s) 825, 1034
FauI CCCGC 1 cut(s) 173
FblI GTMKAC 2 cut(s) 804, 966
Fnu4HI GCNGC 5 cut(s) 575, 767, 1116, 1119, 1140
FokI GGATG 7 cut(s) 341, 394, 599, 667, 950, 970, 1026
FriOI GRGCYC 1 cut(s) 154
Fsp4HI GCNGC 5 cut(s) 575, 767, 1116, 1119, 1140
FspBI CTAG 5 cut(s) 23, 66, 875, 962, 1298
GlaI GCGC 3 cut(s) 193, 1069, 1104
GluI GCNGC 5 cut(s) 575, 767, 1116, 1119, 1140
GsaI CCCAGC 2 cut(s) 116, 860
GsuI CTGGAG 1 cut(s) 819
HaeII RGCGCY 2 cut(s) 195, 1071
HaeIII GGCC 6 cut(s) 207, 702, 723, 901, 982, 1183
HapII CCGG 6 cut(s) 204, 935, 970, 998, 1008, 1264
HhaI GCGC 3 cut(s) 194, 1070, 1105
Hin1II CATG 2 cut(s) 829, 1038
Hin6I GCGC 3 cut(s) 192, 1068, 1103
HinP1I GCGC 3 cut(s) 192, 1068, 1103
HindIII AAGCTT 3 cut(s) 533, 1109, 1286
HpaII CCGG 6 cut(s) 204, 935, 970, 998, 1008, 1264
HphI GGTGA 2 cut(s) 39, 289
Hpy166II GTNNAC 2 cut(s) 805, 967
Hpy188I TCNGA 4 cut(s) 149, 356, 907, 1044
Hpy188III TCNNGA 4 cut(s) 368, 665, 1131, 1264
Hpy8I GTNNAC 2 cut(s) 805, 967
HpyAV CCTTC 2 cut(s) 430, 912
HpyCH4III ACNGT 1 cut(s) 314
HpyCH4V TGCA 8 cut(s) 332, 539, 577, 680, 707, 1034, 1115, 1234
HpyF10VI GCNNNNNNNGC 5 cut(s) 200, 209, 871, 1076, 1294
HpyF3I CTNAG 8 cut(s) 80, 353, 399, 594, 606, 648, 672, 1157
Hsp92II CATG 2 cut(s) 829, 1038
HspAI GCGC 3 cut(s) 192, 1068, 1103
Kpn2I TCCGGA 1 cut(s) 1263
KroI GCCGGC 1 cut(s) 203
KroNI GCCGGC 1 cut(s) 205
Kzo9I GATC 3 cut(s) 245, 349, 1122
LmnI GCTCC 2 cut(s) 109, 422
Lsp1109I GCAGC 5 cut(s) 561, 778, 1127, 1130, 1151
LweI GCATC 4 cut(s) 319, 1021, 1048, 1141
MaeI CTAG 5 cut(s) 23, 66, 875, 962, 1298
MaeIII GTNAC 4 cut(s) 270, 388, 889, 1224
MalI GATC 3 cut(s) 247, 351, 1124
MbiI CCGCTC 1 cut(s) 218
MboI GATC 3 cut(s) 245, 349, 1122
MhlI GDGCHC 2 cut(s) 154, 166
MlsI TGGCCA 2 cut(s) 723, 982
MluCI AATT 8 cut(s) 72, 91, 177, 240, 362, 615, 1095, 1321
MluNI TGGCCA 2 cut(s) 723, 982
MlyI GAGTC 3 cut(s) 278, 481, 1136
MmeI TCCRAC 4 cut(s) 296, 322, 1006, 1290
Mox20I TGGCCA 2 cut(s) 723, 982
Mph1103I ATGCAT 1 cut(s) 1036
MroI TCCGGA 1 cut(s) 1263
MroNI GCCGGC 1 cut(s) 203
MscI TGGCCA 2 cut(s) 723, 982
MseI TTAA 1 cut(s) 1293
Msp20I TGGCCA 2 cut(s) 723, 982
MspA1I CMGCKG 2 cut(s) 398, 860
MspI CCGG 6 cut(s) 204, 935, 970, 998, 1008, 1264
MspR9I CCNGG 3 cut(s) 717, 837, 935
Mva1269I GAATGC 1 cut(s) 707
MvaI CCWGG 2 cut(s) 717, 837
MvnI CGCG 1 cut(s) 1105
MwoI GCNNNNNNNGC 5 cut(s) 200, 209, 871, 1076, 1294
NaeI GCCGGC 1 cut(s) 205
NciI CCSGG 1 cut(s) 935
NdeII GATC 3 cut(s) 245, 349, 1122
NgoMIV GCCGGC 1 cut(s) 203
NheI GCTAGC 1 cut(s) 874
NlaIII CATG 2 cut(s) 829, 1038
NlaIV GGNNCC 2 cut(s) 98, 163
NmuCI GTSAC 2 cut(s) 270, 1224
NsiI ATGCAT 1 cut(s) 1036
NspI RCATGY 1 cut(s) 829
NspV TTCGAA 1 cut(s) 277
PctI GAATGC 1 cut(s) 707
PdiI GCCGGC 1 cut(s) 205
PfeI GAWTC 7 cut(s) 198, 631, 745, 1088, 1188, 1243, 1345
PinAI ACCGGT 1 cut(s) 969
PkrI GCNGC 5 cut(s) 576, 768, 1117, 1120, 1141
Ple19I CGATCG 1 cut(s) 1125
PleI GAGTC 3 cut(s) 277, 481, 1135
PpsI GAGTC 3 cut(s) 277, 481, 1135
Psp124BI GAGCTC 1 cut(s) 154
Psp6I CCWGG 2 cut(s) 715, 835
PspFI CCCAGC 2 cut(s) 112, 856
PspGI CCWGG 2 cut(s) 715, 835
PspN4I GGNNCC 2 cut(s) 98, 163
PspPI GGNCC 3 cut(s) 517, 1133, 1301
PvuI CGATCG 1 cut(s) 1125
PvuII CAGCTG 2 cut(s) 398, 860
RsaI GTAC 3 cut(s) 477, 592, 1020
RsaNI GTAC 3 cut(s) 476, 591, 1019
Rsr2I CGGWCCG 1 cut(s) 517
RsrII CGGWCCG 1 cut(s) 517
SacI GAGCTC 1 cut(s) 154
SaqAI TTAA 1 cut(s) 1293
SatI GCNGC 5 cut(s) 575, 767, 1116, 1119, 1140
Sau3AI GATC 3 cut(s) 245, 349, 1122
Sau96I GGNCC 3 cut(s) 517, 1133, 1301
ScaI AGTACT 1 cut(s) 477
SchI GAGTC 3 cut(s) 278, 481, 1136
ScrFI CCNGG 3 cut(s) 717, 837, 935
SduI GDGCHC 2 cut(s) 154, 166
SfaNI GCATC 4 cut(s) 319, 1021, 1048, 1141
SfcI CTRYAG 2 cut(s) 642, 1340
SfuI TTCGAA 1 cut(s) 277
SinI GGWCC 3 cut(s) 517, 1133, 1301
SmlI CTYRAG 1 cut(s) 482
SmoI CTYRAG 1 cut(s) 482
SpeI ACTAGT 1 cut(s) 961
Sse9I AATT 8 cut(s) 72, 91, 177, 240, 362, 615, 1095, 1321
SsiI CCGC 4 cut(s) 166, 216, 515, 1196
SspMI CTAG 5 cut(s) 23, 66, 875, 962, 1298
SstI GAGCTC 1 cut(s) 154
StyD4I CCNGG 3 cut(s) 715, 835, 933
StyI CCWWGG 1 cut(s) 508
TaaI ACNGT 1 cut(s) 314
TaqI TCGA 4 cut(s) 277, 952, 1125, 1186
TaqII GACCGA 1 cut(s) 624
TasI AATT 8 cut(s) 72, 91, 177, 240, 362, 615, 1095, 1321
TatI WGTACW 2 cut(s) 475, 1018
TfiI GAWTC 7 cut(s) 198, 631, 745, 1088, 1188, 1243, 1345
Tru1I TTAA 1 cut(s) 1293
Tru9I TTAA 1 cut(s) 1293
TscAI CASTG 2 cut(s) 289, 856
TseFI GTSAC 2 cut(s) 270, 1224
TseI GCWGC 5 cut(s) 574, 766, 1115, 1118, 1139
Tsp45I GTSAC 2 cut(s) 270, 1224
TspDTI ATGAA 1 cut(s) 456
TspGWI ACGGA 2 cut(s) 509, 1182
TspRI CASTG 2 cut(s) 289, 856
VpaK11BI GGWCC 3 cut(s) 517, 1133, 1301
XapI RAATTY 1 cut(s) 1321
XceI RCATGY 1 cut(s) 829
XmiI GTMKAC 2 cut(s) 804, 966
XspI CTAG 5 cut(s) 23, 66, 875, 962, 1298
ZrmI AGTACT 1 cut(s) 477
Zsp2I ATGCAT 1 cut(s) 1036
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.