AT2G37585

Core-2/I-Branching enzyme

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
2
OG0015805
Physical Location & Seq
Forward (+)
15765698 .. 15767878
2181 bp
Loading structure...
UTR
Exon/CDS
Intron
AT2G37585.1

Sequence Viewer

Length: 1155 bp
ATGAAAAGATCTCACATTTCGTCTCCTCGTTCTTATTCACGACCAGCGATTTCAATCTTCGGTGTCTTCCTCTTGTTTCTTCTGGTTTTAACACTAAGCTCTCGAAAACCATCAGATTCATCCTCCGGTTTAGCTCCAAATCGAAACTTAGCCACAAAATCGACGATTCCGAGGTTTGCGTATTTGGTAACGGGAACGAAAGGAGATGGGAAACGAGTGAAGCGGCTGCTTAAAGCAATACATCATCCGAGAAATTACTATCTGCTTCATTTGGATCTCGAAGCTTCAGATGAAGAGAGAATGGAGCTGGCGAAGTATGTGAGATCGGAGAAGAAGAAGTTTGAGAATGTTATGGTAATGGGTTTAGCTGATTTGGTTACGGAGAAAGGTCCAACGATGTTAGCTAGTACGCTTCATGGTGTTGCCATTTTGTTGAAGAAAGCTAAGGATTGGGATTGGTTTATTAATCTTAGTGCTTCTGATTATCCTTTAATGCCTCAAGATGATATTTTGCATATATTCTCATACTTGCCACGCTACCTGAACTTTATCGAGCACACAAGCAACATCGGTTGGAAAGAGAACCAAAGAGCAAGGCCTATCATTATTGATCCTGGTTTTTACCATTTGAAGAAATCTGGTGTCTTTTGGGCTAAAGAACGGAGATCTTTGCCTGCTTCATTCAAACTTTTCATGGGTTCAACCAGCGTTGCTTTAACAAGACCGTTCCTCGAGTTTTGCATTTGGGGATGGGATAATCTCCCAAGAACGCTATTGATGTACTATACCAACTTCCTCTTGTCATCTGAAGGCTACTTTCAGACAGTTGTGTGCAACAATAAAGATTATCAGAACACAACGGTTAACCACGACCTGCATTACACAAAATGGGATCCGCTTCAACAGCGCACCCTGAATGTAACGGTTGAGAATTTCCGCGATATGGTCCAAAGCGGGGCTCCATTCGCAAGGGAGTTTCGAGAGGATGATCTTGTACTCGATAAGATTGACATTGAGCTACTAGGTCAAACCGATACTGGATTGGAGTTGAAGACTCCGGATGTTGTTAAACCAACGGTTAGTTGGAAGAGGCTTGAGAAGCTCATGGTTAGGCTTCTTGATCATGAGAATTTCAGGGCTAAGCAATGTAAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0008194 GO:0015020 GO:0016740 GO:0016757 GO:0016758
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Branch

Protein Analysis

384

Amino Acids

44.46

Weight (kDa)

9.41

Isoelectric Point (pI)

40.46

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Branch PF02485 59 - 315 6.4e-68 Core-2/I-Branching enzyme
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015805)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G37585
fragaria_vesca FvH4_6g14020 FvH4_6g14020
malus_domestica MD12G1078600.v1.1
prunus_persica Prupe.6G253100_v2.0.a1 Prupe.6G253100_v2.0.a1
pyrus_communis pycom12g13490
rosa_multiflora Rmu_co8144344.1_g000001
rosa_roxburghii Rroxscaffold_6G00414080
rosa_rugosa Rorug03G0084200
rosa_samantha Rh3AG131800 Rh3BG152900 Rh3CG153100 Rh3DG154000
rosa_wichuraiana Rw3G012460

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 882
AccII CGCG 1 cut(s) 939
AccIII TCCGGA 1 cut(s) 1057
AciI CCGC 4 cut(s) 223, 896, 937, 954
AclWI GGATC 4 cut(s) 282, 605, 887, 900
AcsI RAATTY 2 cut(s) 931, 1129
AcuI CTGAAG 2 cut(s) 270, 828
AfaI GTAC 3 cut(s) 409, 782, 996
AfiI CCNNNNNNNGG 2 cut(s) 943, 955
AgsI TTSAA 7 cut(s) 54, 436, 631, 685, 702, 902, 1051
AjnI CCWGG 1 cut(s) 613
AluBI AGCT 9 cut(s) 99, 134, 284, 307, 368, 404, 443, 1018, 1102
AluI AGCT 9 cut(s) 99, 134, 284, 307, 368, 404, 443, 1018, 1102
Alw21I GWGCWC 1 cut(s) 558
Alw26I GTCTC 1 cut(s) 27
AlwI GGATC 4 cut(s) 282, 605, 887, 900
Ama87I CYCGRG 1 cut(s) 731
Aor13HI TCCGGA 1 cut(s) 1057
AoxI GGCC 1 cut(s) 596
ApeKI GCWGC 1 cut(s) 226
ApoI RAATTY 2 cut(s) 931, 1129
AseI ATTAAT 1 cut(s) 465
AspLEI GCGC 1 cut(s) 909
AspS9I GGNCC 2 cut(s) 389, 946
AvaI CYCGRG 1 cut(s) 731
AvaII GGWCC 2 cut(s) 389, 946
BamHI GGATCC 1 cut(s) 892
BanII GRGCYC 1 cut(s) 961
BbsI GAAGAC 2 cut(s) 58, 1058
Bbv12I GWGCWC 1 cut(s) 558
BbvI GCAGC 1 cut(s) 213
BccI CCATC 3 cut(s) 118, 200, 744
BciT130I CCWGG 1 cut(s) 615
BclI TGATCA 1 cut(s) 1120
BcoDI GTCTC 1 cut(s) 27
BfaI CTAG 2 cut(s) 405, 1022
BfuAI ACCTGC 1 cut(s) 882
BglII AGATCT 2 cut(s) 8, 665
BisI GCNGC 2 cut(s) 224, 227
BlpI GCTNAGC 1 cut(s) 1140
BlsI GCNGC 2 cut(s) 225, 228
Bme1390I CCNGG 1 cut(s) 615
Bme18I GGWCC 2 cut(s) 389, 946
BmeT110I CYCGRG 1 cut(s) 731
BmgT120I GGNCC 2 cut(s) 389, 946
BmiI GGNNCC 2 cut(s) 894, 960
BmrFI CCNGG 1 cut(s) 615
BpiI GAAGAC 2 cut(s) 58, 1058
Bpu10I CCTNAGC 1 cut(s) 444
Bpu1102I GCTNAGC 1 cut(s) 1140
BpuEI CTTGAG 2 cut(s) 483, 1115
BsaBI GATNNNNATC 1 cut(s) 53
BsaJI CCNNGG 1 cut(s) 170
BsaWI WCCGGW 2 cut(s) 125, 1057
Bsc4I CCNNNNNNNGG 2 cut(s) 943, 955
Bse1I ACTGG 1 cut(s) 1042
Bse3DI GCAATG 1 cut(s) 1151
Bse8I GATNNNNATC 1 cut(s) 53
BseAI TCCGGA 1 cut(s) 1057
BseBI CCWGG 1 cut(s) 615
BseDI CCNNGG 1 cut(s) 170
BseGI GGATG 5 cut(s) 119, 244, 755, 991, 1066
BseJI GATNNNNATC 1 cut(s) 53
BseLI CCNNNNNNNGG 2 cut(s) 943, 955
BseMI GCAATG 1 cut(s) 1151
BseNI ACTGG 1 cut(s) 1042
BseRI GAGGAG 1 cut(s) 15
BseXI GCAGC 1 cut(s) 213
Bsh1236I CGCG 1 cut(s) 939
BshFI GGCC 1 cut(s) 598
BsiHKAI GWGCWC 1 cut(s) 558
BsiHKCI CYCGRG 1 cut(s) 731
BsiSI CCGG 2 cut(s) 126, 1058
BslI CCNNNNNNNGG 2 cut(s) 943, 955
BsmAI GTCTC 1 cut(s) 27
BsmBI CGTCTC 1 cut(s) 27
BsnI GGCC 1 cut(s) 598
BsoBI CYCGRG 1 cut(s) 731
Bsp1286I GDGCHC 2 cut(s) 558, 961
Bsp13I TCCGGA 1 cut(s) 1057
Bsp143I GATC 8 cut(s) 8, 274, 323, 610, 665, 892, 988, 1120
Bsp1720I GCTNAGC 1 cut(s) 1140
BspACI CCGC 4 cut(s) 223, 896, 937, 954
BspANI GGCC 1 cut(s) 598
BspEI TCCGGA 1 cut(s) 1057
BspFNI CGCG 1 cut(s) 939
BspHI TCATGA 1 cut(s) 1123
BspLI GGNNCC 2 cut(s) 894, 960
BspMI ACCTGC 1 cut(s) 882
BspPI GGATC 4 cut(s) 282, 605, 887, 900
BsrDI GCAATG 1 cut(s) 1151
BsrI ACTGG 1 cut(s) 1042
BssECI CCNNGG 1 cut(s) 170
BssMI GATC 8 cut(s) 8, 274, 323, 610, 665, 892, 988, 1120
Bst2UI CCWGG 1 cut(s) 615
Bst4CI ACNGT 5 cut(s) 726, 826, 862, 925, 1078
Bst6I CTCTTC 2 cut(s) 288, 1082
BstC8I GCNNGC 2 cut(s) 309, 675
BstDEI CTNAG 5 cut(s) 95, 148, 444, 470, 1140
BstF5I GGATG 5 cut(s) 119, 244, 755, 991, 1066
BstFNI CGCG 1 cut(s) 939
BstHHI GCGC 1 cut(s) 909
BstKTI GATC 8 cut(s) 11, 277, 326, 613, 668, 895, 991, 1123
BstMAI GTCTC 1 cut(s) 27
BstMBI GATC 8 cut(s) 8, 274, 323, 610, 665, 892, 988, 1120
BstMWI GCNNNNNNNGC 3 cut(s) 904, 965, 1099
BstNI CCWGG 1 cut(s) 615
BstSCI CCNGG 1 cut(s) 613
BstUI CGCG 1 cut(s) 939
BstV1I GCAGC 1 cut(s) 213
BstV2I GAAGAC 2 cut(s) 58, 1058
BstX2I RGATCY 4 cut(s) 8, 274, 665, 892
BstYI RGATCY 4 cut(s) 8, 274, 665, 892
BsuRI GGCC 1 cut(s) 598
BtsCI GGATG 5 cut(s) 119, 244, 755, 991, 1066
BveI ACCTGC 1 cut(s) 882
Cac8I GCNNGC 2 cut(s) 309, 675
CciI TCATGA 1 cut(s) 1123
CfoI GCGC 1 cut(s) 909
Cfr13I GGNCC 2 cut(s) 389, 946
Csp6I GTAC 3 cut(s) 408, 781, 995
CviAII CATG 4 cut(s) 416, 694, 1105, 1124
CviQI GTAC 3 cut(s) 408, 781, 995
DdeI CTNAG 5 cut(s) 95, 148, 444, 470, 1140
DpnI GATC 8 cut(s) 10, 276, 325, 612, 667, 894, 990, 1122
DpnII GATC 8 cut(s) 8, 274, 323, 610, 665, 892, 988, 1120
Eam1104I CTCTTC 2 cut(s) 288, 1082
EarI CTCTTC 2 cut(s) 288, 1082
Eco147I AGGCCT 1 cut(s) 598
Eco24I GRGCYC 1 cut(s) 961
Eco47I GGWCC 2 cut(s) 389, 946
Eco57I CTGAAG 2 cut(s) 270, 828
Eco88I CYCGRG 1 cut(s) 731
EcoRII CCWGG 1 cut(s) 613
EcoT38I GRGCYC 1 cut(s) 961
Esp3I CGTCTC 1 cut(s) 27
FaeI CATG 4 cut(s) 419, 697, 1108, 1127
FatI CATG 4 cut(s) 415, 693, 1104, 1123
FauI CCCGC 1 cut(s) 947
FbaI TGATCA 1 cut(s) 1120
Fnu4HI GCNGC 2 cut(s) 224, 227
FokI GGATG 5 cut(s) 106, 231, 762, 998, 1073
FriOI GRGCYC 1 cut(s) 961
Fsp4HI GCNGC 2 cut(s) 224, 227
FspBI CTAG 2 cut(s) 405, 1022
GlaI GCGC 1 cut(s) 908
GluI GCNGC 2 cut(s) 224, 227
HaeIII GGCC 1 cut(s) 598
HapII CCGG 2 cut(s) 126, 1058
HhaI GCGC 1 cut(s) 909
Hin1II CATG 4 cut(s) 419, 697, 1108, 1127
Hin6I GCGC 1 cut(s) 907
HinP1I GCGC 1 cut(s) 907
HincII GTYRAC 1 cut(s) 865
HindII GTYRAC 1 cut(s) 865
HindIII AAGCTT 1 cut(s) 282
HinfI GANTC 3 cut(s) 116, 166, 1054
HpaI GTTAAC 1 cut(s) 865
HpaII CCGG 2 cut(s) 126, 1058
Hpy166II GTNNAC 1 cut(s) 865
Hpy188I TCNGA 9 cut(s) 115, 171, 249, 289, 328, 481, 808, 822, 852
Hpy188III TCNNGA 8 cut(s) 39, 102, 278, 500, 980, 1058, 1118, 1124
Hpy8I GTNNAC 1 cut(s) 865
Hpy99I CGWCG 1 cut(s) 166
HpyAV CCTTC 1 cut(s) 803
HpyCH4III ACNGT 5 cut(s) 726, 826, 862, 925, 1078
HpyCH4V TGCA 4 cut(s) 514, 741, 834, 877
HpyF10VI GCNNNNNNNGC 3 cut(s) 904, 965, 1099
HpyF3I CTNAG 5 cut(s) 95, 148, 444, 470, 1140
Hsp92II CATG 4 cut(s) 419, 697, 1108, 1127
HspAI GCGC 1 cut(s) 907
Kpn2I TCCGGA 1 cut(s) 1057
Ksp22I TGATCA 1 cut(s) 1120
KspAI GTTAAC 1 cut(s) 865
Kzo9I GATC 8 cut(s) 8, 274, 323, 610, 665, 892, 988, 1120
LmnI GCTCC 3 cut(s) 139, 304, 964
Lsp1109I GCAGC 1 cut(s) 213
MaeI CTAG 2 cut(s) 405, 1022
MaeIII GTNAC 3 cut(s) 187, 376, 919
MalI GATC 8 cut(s) 10, 276, 325, 612, 667, 894, 990, 1122
MboI GATC 8 cut(s) 8, 274, 323, 610, 665, 892, 988, 1120
MflI RGATCY 4 cut(s) 8, 274, 665, 892
MhlI GDGCHC 2 cut(s) 558, 961
MluCI AATT 3 cut(s) 253, 931, 1129
MlyI GAGTC 1 cut(s) 1048
MmeI TCCRAC 3 cut(s) 416, 554, 1064
MnlI CCTC 9 cut(s) 36, 80, 133, 165, 507, 740, 806, 976, 1083
MroI TCCGGA 1 cut(s) 1057
MseI TTAA 7 cut(s) 89, 231, 465, 491, 716, 864, 1068
MspI CCGG 2 cut(s) 126, 1058
MspR9I CCNGG 1 cut(s) 615
MvaI CCWGG 1 cut(s) 615
MvnI CGCG 1 cut(s) 939
MwoI GCNNNNNNNGC 3 cut(s) 904, 965, 1099
NdeII GATC 8 cut(s) 8, 274, 323, 610, 665, 892, 988, 1120
NlaIII CATG 4 cut(s) 419, 697, 1108, 1127
NlaIV GGNNCC 2 cut(s) 894, 960
PaeR7I CTCGAG 1 cut(s) 731
PagI TCATGA 1 cut(s) 1123
PceI AGGCCT 1 cut(s) 598
PcsI WCGNNNNNNNCGW 1 cut(s) 167
PfeI GAWTC 2 cut(s) 116, 166
PkrI GCNGC 2 cut(s) 225, 228
PleI GAGTC 1 cut(s) 1048
PpsI GAGTC 1 cut(s) 1048
PshBI ATTAAT 1 cut(s) 465
Psp6I CCWGG 1 cut(s) 613
PspGI CCWGG 1 cut(s) 613
PspN4I GGNNCC 2 cut(s) 894, 960
PspPI GGNCC 2 cut(s) 389, 946
PspXI VCTCGAGB 1 cut(s) 731
PsuI RGATCY 4 cut(s) 8, 274, 665, 892
RsaI GTAC 3 cut(s) 409, 782, 996
RsaNI GTAC 3 cut(s) 408, 781, 995
SaqAI TTAA 7 cut(s) 89, 231, 465, 491, 716, 864, 1068
SatI GCNGC 2 cut(s) 224, 227
Sau3AI GATC 8 cut(s) 8, 274, 323, 610, 665, 892, 988, 1120
Sau96I GGNCC 2 cut(s) 389, 946
SchI GAGTC 1 cut(s) 1048
ScrFI CCNGG 1 cut(s) 615
SduI GDGCHC 2 cut(s) 558, 961
Sfr274I CTCGAG 1 cut(s) 731
SinI GGWCC 2 cut(s) 389, 946
SlaI CTCGAG 1 cut(s) 731
SmlI CTYRAG 3 cut(s) 498, 731, 1094
SmoI CTYRAG 3 cut(s) 498, 731, 1094
Sse9I AATT 3 cut(s) 253, 931, 1129
SseBI AGGCCT 1 cut(s) 598
SsiI CCGC 4 cut(s) 223, 896, 937, 954
SspMI CTAG 2 cut(s) 405, 1022
StuI AGGCCT 1 cut(s) 598
StyD4I CCNGG 1 cut(s) 613
TaaI ACNGT 5 cut(s) 726, 826, 862, 925, 1078
TaqI TCGA 8 cut(s) 103, 142, 161, 279, 552, 732, 979, 999
TasI AATT 3 cut(s) 253, 931, 1129
TatI WGTACW 2 cut(s) 780, 994
TauI GCSGC 1 cut(s) 226
TfiI GAWTC 2 cut(s) 116, 166
Tru1I TTAA 7 cut(s) 89, 231, 465, 491, 716, 864, 1068
Tru9I TTAA 7 cut(s) 89, 231, 465, 491, 716, 864, 1068
TseI GCWGC 1 cut(s) 226
TspDTI ATGAA 7 cut(s) 17, 108, 257, 306, 404, 669, 682
TspGWI ACGGA 2 cut(s) 395, 676
VpaK11BI GGWCC 2 cut(s) 389, 946
VspI ATTAAT 1 cut(s) 465
XapI RAATTY 2 cut(s) 931, 1129
XcmI CCANNNNNNNNNTGG 1 cut(s) 1080
XhoI CTCGAG 1 cut(s) 731
XspI CTAG 2 cut(s) 405, 1022
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.