Rmu_co8144344.1_g000001

Core-2/I-Branching enzyme

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_co8144344.1
OG0015805
Physical Location & Seq
Forward (+)
1 .. 455
455 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_co8144344.1_g000001.1.cds

Sequence Viewer

Length: 455 bp
ccggccccgacttcacctacgaccaccgccgattccctaatcccgaccagtatttcgtcgacagcgacgacgacgacgagaactacggcctagggctcccgagattcgcttacctgattaccggttctaaaggtgacggccctcagctgaagcggctgcttcaggcggcgtaccacccgagaaattactacttgctccaactcgatctggaagcttccgacgccgagaggctcgagctcgccaagtacgtcaagtccgagagtgtgatgaacgcgtttcggaatgcgatggtggttggaaccgcggatttggtgaccgcgaaaggtccgacggtgttggcggcgacgctgcacgcggttgcgattttgctcaagcgggccgaggattgggattggtttattaatctcagtgcttcggattatccgctcatgtcccaagatggtgagttttactaa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0008194 GO:0015020 GO:0016740 GO:0016757 GO:0016758
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Branch

Protein Analysis

150

Amino Acids

16.96

Weight (kDa)

4.64

Isoelectric Point (pI)

35.32

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015805)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G37585
fragaria_vesca FvH4_6g14020 FvH4_6g14020
malus_domestica MD12G1078600.v1.1
prunus_persica Prupe.6G253100_v2.0.a1 Prupe.6G253100_v2.0.a1
pyrus_communis pycom12g13490
rosa_multiflora Rmu_co8144344.1_g000001
rosa_roxburghii Rroxscaffold_6G00414080
rosa_rugosa Rorug03G0084200
rosa_samantha Rh3AG131800 Rh3BG152900 Rh3CG153100 Rh3DG154000
rosa_wichuraiana Rw3G012460

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 426
AccI GTMKAC 1 cut(s) 59
AccII CGCG 4 cut(s) 274, 304, 319, 355
AcuI CTGAAG 2 cut(s) 145, 169
AcyI GRCGYC 1 cut(s) 221
AfaI GTAC 2 cut(s) 172, 247
AfiI CCNNNNNNNGG 1 cut(s) 386
AflIII ACRYGT 1 cut(s) 272
AgeI ACCGGT 1 cut(s) 121
AluBI AGCT 3 cut(s) 147, 214, 237
AluI AGCT 3 cut(s) 147, 214, 237
Alw21I GWGCWC 1 cut(s) 239
Ama87I CYCGRG 3 cut(s) 99, 177, 232
AoxI GGCC 4 cut(s) 3, 87, 138, 377
ApeKI GCWGC 2 cut(s) 156, 348
ArsI GACNNNNNNTTYG 2 cut(s) 37, 69
AseI ATTAAT 1 cut(s) 401
AsiGI ACCGGT 1 cut(s) 121
AspA2I CCTAGG 1 cut(s) 90
AspS9I GGNCC 4 cut(s) 4, 139, 325, 377
AsuHPI GGTGA 4 cut(s) 6, 145, 324, 454
AvaI CYCGRG 3 cut(s) 99, 177, 232
AvaII GGWCC 1 cut(s) 325
AvrII CCTAGG 1 cut(s) 90
BanII GRGCYC 2 cut(s) 98, 239
Bbv12I GWGCWC 1 cut(s) 239
BbvCI CCTCAGC 1 cut(s) 143
BbvI GCAGC 2 cut(s) 143, 335
BccI CCATC 2 cut(s) 282, 433
BceAI ACGGC 2 cut(s) 102, 153
BfaI CTAG 1 cut(s) 91
BisI GCNGC 5 cut(s) 154, 157, 167, 341, 349
BlnI CCTAGG 1 cut(s) 90
BlsI GCNGC 5 cut(s) 155, 158, 168, 342, 350
Bme18I GGWCC 1 cut(s) 325
BmeT110I CYCGRG 3 cut(s) 99, 177, 232
BmgT120I GGNCC 4 cut(s) 4, 139, 325, 377
BmiI GGNNCC 3 cut(s) 6, 97, 300
Bpu10I CCTNAGC 1 cut(s) 143
BpuEI CTTGAG 1 cut(s) 355
BsaHI GRCGYC 1 cut(s) 221
BsaJI CCNNGG 3 cut(s) 90, 302, 380
BsaWI WCCGGW 1 cut(s) 121
Bsc4I CCNNNNNNNGG 1 cut(s) 386
Bse118I RCCGGY 1 cut(s) 121
Bse1I ACTGG 1 cut(s) 48
BseDI CCNNGG 3 cut(s) 90, 302, 380
BseLI CCNNNNNNNGG 1 cut(s) 386
BseMII CTCAG 2 cut(s) 157, 420
BseNI ACTGG 1 cut(s) 48
BseXI GCAGC 2 cut(s) 143, 335
BsgI GTGCAG 1 cut(s) 334
Bsh1236I CGCG 4 cut(s) 274, 304, 319, 355
BshFI GGCC 4 cut(s) 5, 89, 140, 379
BshTI ACCGGT 1 cut(s) 121
BsiHKAI GWGCWC 1 cut(s) 239
BsiHKCI CYCGRG 3 cut(s) 99, 177, 232
BsiSI CCGG 2 cut(s) 2, 122
BslFI GGGAC 1 cut(s) 417
BslI CCNNNNNNNGG 1 cut(s) 386
BsmFI GGGAC 1 cut(s) 417
BsmI GAATGC 1 cut(s) 288
BsnI GGCC 4 cut(s) 5, 89, 140, 379
BsoBI CYCGRG 3 cut(s) 99, 177, 232
Bsp1286I GDGCHC 2 cut(s) 98, 239
Bsp143I GATC 1 cut(s) 204
BspANI GGCC 4 cut(s) 5, 89, 140, 379
BspCNI CTCAG 2 cut(s) 156, 419
BspFNI CGCG 4 cut(s) 274, 304, 319, 355
BspLI GGNNCC 3 cut(s) 6, 97, 300
BsrBI CCGCTC 1 cut(s) 426
BsrFI RCCGGY 1 cut(s) 121
BsrI ACTGG 1 cut(s) 48
BssAI RCCGGY 1 cut(s) 121
BssECI CCNNGG 3 cut(s) 90, 302, 380
BssMI GATC 1 cut(s) 204
BssNI GRCGYC 1 cut(s) 221
BssT1I CCWWGG 1 cut(s) 90
Bst4CI ACNGT 1 cut(s) 333
BstACI GRCGYC 1 cut(s) 221
BstC8I GCNNGC 3 cut(s) 239, 353, 377
BstDEI CTNAG 2 cut(s) 143, 406
BstDSI CCRYGG 1 cut(s) 302
BstEII GGTNACC 1 cut(s) 312
BstFNI CGCG 4 cut(s) 274, 304, 319, 355
BstKTI GATC 1 cut(s) 207
BstMBI GATC 1 cut(s) 204
BstMWI GCNNNNNNNGC 2 cut(s) 153, 220
BstPI GGTNACC 1 cut(s) 312
BstUI CGCG 4 cut(s) 274, 304, 319, 355
BstV1I GCAGC 2 cut(s) 143, 335
BsuRI GGCC 4 cut(s) 5, 89, 140, 379
BtgI CCRYGG 1 cut(s) 302
BtgZI GCGATG 1 cut(s) 301
BtsIMutI CAGTG 1 cut(s) 414
Cac8I GCNNGC 3 cut(s) 239, 353, 377
Cfr10I RCCGGY 1 cut(s) 121
Cfr13I GGNCC 4 cut(s) 4, 139, 325, 377
Cfr42I CCGCGG 1 cut(s) 305
CseI GACGC 2 cut(s) 229, 354
Csp6I GTAC 2 cut(s) 171, 246
CspAI ACCGGT 1 cut(s) 121
CviAII CATG 1 cut(s) 429
CviQI GTAC 2 cut(s) 171, 246
DdeI CTNAG 2 cut(s) 143, 406
DpnI GATC 1 cut(s) 206
DpnII GATC 1 cut(s) 204
Ecl136II GAGCTC 1 cut(s) 237
Eco130I CCWWGG 1 cut(s) 90
Eco24I GRGCYC 2 cut(s) 98, 239
Eco47I GGWCC 1 cut(s) 325
Eco53kI GAGCTC 1 cut(s) 237
Eco57I CTGAAG 2 cut(s) 145, 169
Eco88I CYCGRG 3 cut(s) 99, 177, 232
Eco91I GGTNACC 1 cut(s) 312
EcoICRI GAGCTC 1 cut(s) 237
EcoO65I GGTNACC 1 cut(s) 312
EcoT14I CCWWGG 1 cut(s) 90
EcoT38I GRGCYC 2 cut(s) 98, 239
ErhI CCWWGG 1 cut(s) 90
FaeI CATG 1 cut(s) 432
FaiI YATR 1 cut(s) 430
FaqI GGGAC 1 cut(s) 417
FatI CATG 1 cut(s) 428
FauI CCCGC 1 cut(s) 368
FblI GTMKAC 1 cut(s) 59
Fnu4HI GCNGC 5 cut(s) 154, 157, 167, 341, 349
FriOI GRGCYC 2 cut(s) 98, 239
Fsp4HI GCNGC 5 cut(s) 154, 157, 167, 341, 349
FspBI CTAG 1 cut(s) 91
GluI GCNGC 5 cut(s) 154, 157, 167, 341, 349
HaeIII GGCC 4 cut(s) 5, 89, 140, 379
HapII CCGG 2 cut(s) 2, 122
HgaI GACGC 2 cut(s) 229, 354
Hin1I GRCGYC 1 cut(s) 221
Hin1II CATG 1 cut(s) 432
HincII GTYRAC 1 cut(s) 60
HindII GTYRAC 1 cut(s) 60
HindIII AAGCTT 1 cut(s) 212
HinfI GANTC 2 cut(s) 32, 104
HpaII CCGG 2 cut(s) 2, 122
HphI GGTGA 4 cut(s) 6, 145, 324, 454
Hpy166II GTNNAC 1 cut(s) 60
Hpy188I TCNGA 5 cut(s) 219, 258, 281, 329, 417
Hpy188III TCNNGA 3 cut(s) 43, 99, 208
Hpy8I GTNNAC 1 cut(s) 60
Hpy99I CGWCG 8 cut(s) 61, 70, 73, 76, 79, 223, 333, 348
HpyCH4III ACNGT 1 cut(s) 333
HpyCH4IV ACGT 1 cut(s) 248
HpyCH4V TGCA 1 cut(s) 351
HpyF10VI GCNNNNNNNGC 2 cut(s) 153, 220
HpyF3I CTNAG 2 cut(s) 143, 406
HpySE526I ACGT 1 cut(s) 248
Hsp92I GRCGYC 1 cut(s) 221
Hsp92II CATG 1 cut(s) 432
KspI CCGCGG 1 cut(s) 305
Kzo9I GATC 1 cut(s) 204
LmnI GCTCC 2 cut(s) 101, 200
LpnPI CCDG 6 cut(s) 15, 61, 127, 135, 148, 193
Lsp1109I GCAGC 2 cut(s) 143, 335
MaeI CTAG 1 cut(s) 91
MaeII ACGT 1 cut(s) 248
MaeIII GTNAC 2 cut(s) 133, 312
MalI GATC 1 cut(s) 206
MbiI CCGCTC 1 cut(s) 426
MboI GATC 1 cut(s) 204
MhlI GDGCHC 2 cut(s) 98, 239
MluCI AATT 1 cut(s) 183
MluI ACGCGT 1 cut(s) 272
MmeI TCCRAC 4 cut(s) 222, 242, 276, 352
MnlI CCTC 3 cut(s) 152, 221, 375
MseI TTAA 1 cut(s) 401
MspA1I CMGCKG 2 cut(s) 147, 304
MspI CCGG 2 cut(s) 2, 122
Mva1269I GAATGC 1 cut(s) 288
MvnI CGCG 4 cut(s) 274, 304, 319, 355
MwoI GCNNNNNNNGC 2 cut(s) 153, 220
NdeII GATC 1 cut(s) 204
NlaIII CATG 1 cut(s) 432
NlaIV GGNNCC 3 cut(s) 6, 97, 300
NmeAIII GCCGAG 2 cut(s) 249, 405
NmuCI GTSAC 2 cut(s) 133, 312
PaeR7I CTCGAG 1 cut(s) 232
PcsI WCGNNNNNNNCGW 5 cut(s) 62, 65, 74, 245, 254
PctI GAATGC 1 cut(s) 288
PfeI GAWTC 2 cut(s) 32, 104
PinAI ACCGGT 1 cut(s) 121
PkrI GCNGC 5 cut(s) 155, 158, 168, 342, 350
PshBI ATTAAT 1 cut(s) 401
Psp124BI GAGCTC 1 cut(s) 239
PspEI GGTNACC 1 cut(s) 312
PspN4I GGNNCC 3 cut(s) 6, 97, 300
PspPI GGNCC 4 cut(s) 4, 139, 325, 377
PspXI VCTCGAGB 1 cut(s) 232
PvuII CAGCTG 1 cut(s) 147
RsaI GTAC 2 cut(s) 172, 247
RsaNI GTAC 2 cut(s) 171, 246
SacI GAGCTC 1 cut(s) 239
SacII CCGCGG 1 cut(s) 305
SalI GTCGAC 1 cut(s) 58
SaqAI TTAA 1 cut(s) 401
SatI GCNGC 5 cut(s) 154, 157, 167, 341, 349
Sau3AI GATC 1 cut(s) 204
Sau96I GGNCC 4 cut(s) 4, 139, 325, 377
SduI GDGCHC 2 cut(s) 98, 239
SetI ASST 8 cut(s) 19, 116, 135, 149, 216, 239, 251, 327
Sfr274I CTCGAG 1 cut(s) 232
Sfr303I CCGCGG 1 cut(s) 305
SgrBI CCGCGG 1 cut(s) 305
SinI GGWCC 1 cut(s) 325
SlaI CTCGAG 1 cut(s) 232
SmlI CTYRAG 2 cut(s) 232, 370
SmoI CTYRAG 2 cut(s) 232, 370
Sse9I AATT 1 cut(s) 183
SspMI CTAG 1 cut(s) 91
SstI GAGCTC 1 cut(s) 239
StyI CCWWGG 1 cut(s) 90
TaaI ACNGT 1 cut(s) 333
TaiI ACGT 1 cut(s) 251
TaqI TCGA 3 cut(s) 59, 203, 233
TasI AATT 1 cut(s) 183
TauI GCSGC 3 cut(s) 156, 169, 343
TfiI GAWTC 2 cut(s) 32, 104
Tru1I TTAA 1 cut(s) 401
Tru9I TTAA 1 cut(s) 401
TscAI CASTG 1 cut(s) 414
TseFI GTSAC 2 cut(s) 133, 312
TseI GCWGC 2 cut(s) 156, 348
Tsp45I GTSAC 2 cut(s) 133, 312
TspDTI ATGAA 1 cut(s) 283
TspRI CASTG 1 cut(s) 414
VpaK11BI GGWCC 1 cut(s) 325
VspI ATTAAT 1 cut(s) 401
XhoI CTCGAG 1 cut(s) 232
XmaJI CCTAGG 1 cut(s) 90
XmiI GTMKAC 1 cut(s) 59
XspI CTAG 1 cut(s) 91
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.