FvH4_6g14020

Core-2/I-Branching enzyme

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0015805
Physical Location & Seq
Reverse (-)
8592147 .. 8594716
2570 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g14020.t2

Sequence Viewer

Length: 1251 bp
ATGAAACGAACCTCCAACAACCAGTCCCAATGGCTCGTCATCCTAACCGCAACCTCCGCCCTCCTCCTCCTCGCCCTCCTCGCATTAACCCTAACACACGCCAAGCACTCCTCCTCCGCCGCCGACTACGATCACCGCCGATTCGCTAATCCCGACCACAATTTCATCGACGGCGACAACAGCTACGGCCTCGAGCTCCCGCGCCTCCCGAGATTCGCGTACCTGCTCACCGGTTCCAAAGGCGAAGGCCCGCAGCTGAAGCGGCTGCTCCAGGCGGCGTACCACCCGAGGAATCACTACTTGCTCCACCTCGATCTAGAAGCTTCCGACGCCGAGAGGCTTGACCTGGCTAAGTATGTGAAGTCGGAGAGTGTGATGCACGCGTTTCGGAATGCGATGGTGGTTGGAGCCGCAGATTTGGTGACCGCGAAAGGTCCGACGGTGATGGCGGCGACGCTGCACGCGGTTGCTATTCTGCTGAAGCGGGCTGGGGATTGGGATTGGTTTATTAATCTCAGTGCTTCGGATTATCCACTCATGTCCCAAGATGATTTGTTGCATATCTTTTCATACTTGCCGAGGGATCTTAACTTCCTGGAGCACACTAGCAATATTGGATGGAAAGAGAATCAAAGAGCGAGGCCGATTATTATTGATCCTGGTTTGTACCACAATAAGAAATCAGGTGTGTTTTGGGCTAAAGAGAGAAGGTCAATGCCAGCTTCTTTCAAGTTATTCATGGGGTCTACAGGGGTGGTGCTGACAAGATCATTTCTTGAATTCTGTATTTTGGGATGGGACAATCTTCCCCGCACTCTTCTCATGTACTATACTAATTTCCTTCAGTCACCAGAAGGCTACTTCCACACTGTTATCTGCAACCATAAGGATTACCAGAACACAACTGTAAATCATGACTTGCATTACATAAGGTGGGATAGCCCTCCAAAGCTAAATCCAGTAAATCTAACACTGGAACATTACAATGACATCATCCAAGCGGGAGCTCCTCTTGCCGGTCCATTTACAAGGGATGATCCAGTTCTCGACAAAATTGATAAGGAGCTCTTGGGGAGACCAAGCGGGCATCTGACACCCGGTGGTTGGTGTTTAGGAAGAGTTGGTAAAGATAGATGTTCAGTTTATGGGAATCCAGTGGCTGTAAAACCTAGTGTAGTTTCAAAGAGGCTAGAGAAACTCATTGTGAAACTTCTTGATTCTGAAAATTTCAGGTCTAAGCAGTGTAAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0008194 GO:0015020 GO:0016740 GO:0016757 GO:0016758
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Branch

Protein Analysis

417

Amino Acids

47.02

Weight (kDa)

8.99

Isoelectric Point (pI)

45.01

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Branch PF02485 72 - 312 2.3e-64 Core-2/I-Branching enzyme
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015805)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G37585
fragaria_vesca FvH4_6g14020 FvH4_6g14020
malus_domestica MD12G1078600.v1.1
prunus_persica Prupe.6G253100_v2.0.a1 Prupe.6G253100_v2.0.a1
pyrus_communis pycom12g13490
rosa_multiflora Rmu_co8144344.1_g000001
rosa_roxburghii Rroxscaffold_6G00414080
rosa_rugosa Rorug03G0084200
rosa_samantha Rh3AG131800 Rh3BG152900 Rh3CG153100 Rh3DG154000
rosa_wichuraiana Rw3G012460

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 231
AccI GTMKAC 1 cut(s) 746
AccII CGCG 5 cut(s) 202, 218, 383, 428, 464
AclWI GGATC 3 cut(s) 591, 650, 1031
AcsI RAATTY 2 cut(s) 779, 1225
AcuI CTGAAG 3 cut(s) 278, 500, 827
AcyI GRCGYC 1 cut(s) 330
AdeI CACNNNGTG 1 cut(s) 1100
AfaI GTAC 4 cut(s) 221, 281, 668, 827
AfiI CCNNNNNNNGG 2 cut(s) 1016, 1104
AflIII ACRYGT 1 cut(s) 381
AgeI ACCGGT 1 cut(s) 230
AgsI TTSAA 3 cut(s) 730, 779, 1182
AjnI CCWGG 4 cut(s) 270, 345, 594, 658
AluBI AGCT 8 cut(s) 183, 196, 256, 323, 722, 952, 1007, 1066
AluI AGCT 8 cut(s) 183, 196, 256, 323, 722, 952, 1007, 1066
Alw21I GWGCWC 4 cut(s) 198, 603, 1009, 1068
Alw26I GTCTC 1 cut(s) 1069
AlwI GGATC 3 cut(s) 591, 650, 1031
AlwNI CAGNNNCTG 1 cut(s) 1160
Ama87I CYCGRG 3 cut(s) 191, 208, 286
AoxI GGCC 3 cut(s) 187, 247, 641
ApeKI GCWGC 3 cut(s) 253, 265, 457
ApoI RAATTY 2 cut(s) 779, 1225
AseI ATTAAT 1 cut(s) 510
AsiGI ACCGGT 1 cut(s) 230
AspLEI GCGC 1 cut(s) 204
AspS9I GGNCC 3 cut(s) 248, 434, 1019
AsuC2I CCSGG 1 cut(s) 1098
AsuHPI GGTGA 5 cut(s) 125, 220, 433, 454, 840
AvaI CYCGRG 3 cut(s) 191, 208, 286
AvaII GGWCC 2 cut(s) 434, 1019
BanII GRGCYC 3 cut(s) 198, 1009, 1068
Bbv12I GWGCWC 4 cut(s) 198, 603, 1009, 1068
BbvI GCAGC 3 cut(s) 252, 265, 444
BccI CCATC 4 cut(s) 391, 439, 612, 789
BceAI ACGGC 2 cut(s) 187, 202
BcgI CGANNNNNNTGC 2 cut(s) 368, 402
BciT130I CCWGG 4 cut(s) 272, 347, 596, 660
BcnI CCSGG 1 cut(s) 1098
BcoDI GTCTC 1 cut(s) 1069
BfaI CTAG 4 cut(s) 317, 606, 1170, 1190
BfmI CTRYAG 1 cut(s) 747
BfuAI ACCTGC 1 cut(s) 231
BisI GCNGC 8 cut(s) 120, 254, 263, 266, 276, 411, 450, 458
BlsI GCNGC 8 cut(s) 121, 255, 264, 267, 277, 412, 451, 459
Bme1390I CCNGG 5 cut(s) 272, 347, 596, 660, 1098
Bme18I GGWCC 2 cut(s) 434, 1019
BmeT110I CYCGRG 3 cut(s) 191, 208, 286
BmgT120I GGNCC 3 cut(s) 248, 434, 1019
BmiI GGNNCC 2 cut(s) 235, 409
BmrFI CCNGG 5 cut(s) 272, 347, 596, 660, 1098
BmsI GCATC 2 cut(s) 366, 1096
BpmI CTGGAG 2 cut(s) 254, 617
BpuMI CCSGG 1 cut(s) 1098
BsaHI GRCGYC 1 cut(s) 330
BsaI GGTCTC 1 cut(s) 1069
BsaJI CCNNGG 2 cut(s) 287, 578
BsaWI WCCGGW 1 cut(s) 230
BsaXI ACNNNNNCTCC 2 cut(s) 98, 128
Bsc4I CCNNNNNNNGG 2 cut(s) 1016, 1104
Bse118I RCCGGY 2 cut(s) 230, 1016
Bse1I ACTGG 5 cut(s) 22, 959, 978, 1040, 1154
BseBI CCWGG 4 cut(s) 272, 347, 596, 660
BseDI CCNNGG 2 cut(s) 287, 578
BseGI GGATG 5 cut(s) 39, 623, 800, 993, 1039
BseLI CCNNNNNNNGG 2 cut(s) 1016, 1104
BseMII CTCAG 1 cut(s) 529
BseNI ACTGG 5 cut(s) 22, 959, 978, 1040, 1154
BseRI GAGGAG 7 cut(s) 53, 56, 59, 68, 100, 103, 999
BseXI GCAGC 3 cut(s) 252, 265, 444
BseYI CCCAGC 1 cut(s) 488
BsgI GTGCAG 1 cut(s) 443
Bsh1236I CGCG 5 cut(s) 202, 218, 383, 428, 464
BshFI GGCC 3 cut(s) 189, 249, 643
BshTI ACCGGT 1 cut(s) 230
BsiHKAI GWGCWC 4 cut(s) 198, 603, 1009, 1068
BsiHKCI CYCGRG 3 cut(s) 191, 208, 286
BsiSI CCGG 3 cut(s) 231, 1017, 1098
BslFI GGGAC 3 cut(s) 10, 526, 812
BslI CCNNNNNNNGG 2 cut(s) 1016, 1104
BsmAI GTCTC 1 cut(s) 1069
BsmFI GGGAC 3 cut(s) 10, 526, 812
BsmI GAATGC 1 cut(s) 397
BsnI GGCC 3 cut(s) 189, 249, 643
Bso31I GGTCTC 1 cut(s) 1069
BsoBI CYCGRG 3 cut(s) 191, 208, 286
Bsp1286I GDGCHC 4 cut(s) 198, 603, 1009, 1068
Bsp143I GATC 6 cut(s) 130, 313, 583, 655, 767, 1036
BspANI GGCC 3 cut(s) 189, 249, 643
BspCNI CTCAG 1 cut(s) 528
BspFNI CGCG 5 cut(s) 202, 218, 383, 428, 464
BspHI TCATGA 1 cut(s) 913
BspLI GGNNCC 2 cut(s) 235, 409
BspMI ACCTGC 1 cut(s) 231
BspPI GGATC 3 cut(s) 591, 650, 1031
BspTNI GGTCTC 1 cut(s) 1069
BsrFI RCCGGY 2 cut(s) 230, 1016
BsrI ACTGG 5 cut(s) 22, 959, 978, 1040, 1154
BssAI RCCGGY 2 cut(s) 230, 1016
BssECI CCNNGG 2 cut(s) 287, 578
BssMI GATC 6 cut(s) 130, 313, 583, 655, 767, 1036
BssNI GRCGYC 1 cut(s) 330
Bst2UI CCWGG 4 cut(s) 272, 347, 596, 660
Bst4CI ACNGT 3 cut(s) 442, 871, 907
Bst6I CTCTTC 2 cut(s) 822, 1111
BstACI GRCGYC 1 cut(s) 330
BstC8I GCNNGC 6 cut(s) 251, 381, 462, 486, 720, 1085
BstDEI CTNAG 3 cut(s) 351, 515, 1236
BstEII GGTNACC 1 cut(s) 421
BstF5I GGATG 5 cut(s) 39, 623, 800, 993, 1039
BstFNI CGCG 5 cut(s) 202, 218, 383, 428, 464
BstHHI GCGC 1 cut(s) 204
BstKTI GATC 6 cut(s) 133, 316, 586, 658, 770, 1039
BstMAI GTCTC 1 cut(s) 1069
BstMBI GATC 6 cut(s) 130, 313, 583, 655, 767, 1036
BstMWI GCNNNNNNNGC 7 cut(s) 56, 80, 180, 259, 262, 329, 1013
BstNI CCWGG 4 cut(s) 272, 347, 596, 660
BstPI GGTNACC 1 cut(s) 421
BstSCI CCNGG 5 cut(s) 270, 345, 594, 658, 1096
BstSFI CTRYAG 1 cut(s) 747
BstUI CGCG 5 cut(s) 202, 218, 383, 428, 464
BstV1I GCAGC 3 cut(s) 252, 265, 444
BstX2I RGATCY 1 cut(s) 583
BstYI RGATCY 1 cut(s) 583
BsuRI GGCC 3 cut(s) 189, 249, 643
BtgZI GCGATG 1 cut(s) 410
BtsCI GGATG 5 cut(s) 39, 623, 800, 993, 1039
BtsI GCAGTG 1 cut(s) 1247
BtsIMutI CAGTG 5 cut(s) 523, 867, 971, 1161, 1247
BveI ACCTGC 1 cut(s) 231
Cac8I GCNNGC 6 cut(s) 251, 381, 462, 486, 720, 1085
CaiI CAGNNNCTG 1 cut(s) 1160
CciI TCATGA 1 cut(s) 913
CfoI GCGC 1 cut(s) 204
Cfr10I RCCGGY 2 cut(s) 230, 1016
Cfr13I GGNCC 3 cut(s) 248, 434, 1019
CseI GACGC 2 cut(s) 338, 463
Csp6I GTAC 4 cut(s) 220, 280, 667, 826
CspAI ACCGGT 1 cut(s) 230
CviAII CATG 4 cut(s) 538, 739, 823, 914
CviQI GTAC 4 cut(s) 220, 280, 667, 826
DdeI CTNAG 3 cut(s) 351, 515, 1236
DpnI GATC 6 cut(s) 132, 315, 585, 657, 769, 1038
DpnII GATC 6 cut(s) 130, 313, 583, 655, 767, 1036
DraIII CACNNNGTG 1 cut(s) 1100
Eam1104I CTCTTC 2 cut(s) 822, 1111
EarI CTCTTC 2 cut(s) 822, 1111
EciI GGCGGA 2 cut(s) 46, 106
Ecl136II GAGCTC 3 cut(s) 196, 1007, 1066
Eco24I GRGCYC 3 cut(s) 198, 1009, 1068
Eco31I GGTCTC 1 cut(s) 1069
Eco47I GGWCC 2 cut(s) 434, 1019
Eco53kI GAGCTC 3 cut(s) 196, 1007, 1066
Eco57I CTGAAG 3 cut(s) 278, 500, 827
Eco88I CYCGRG 3 cut(s) 191, 208, 286
Eco91I GGTNACC 1 cut(s) 421
EcoICRI GAGCTC 3 cut(s) 196, 1007, 1066
EcoO65I GGTNACC 1 cut(s) 421
EcoRI GAATTC 1 cut(s) 779
EcoRII CCWGG 4 cut(s) 270, 345, 594, 658
EcoT38I GRGCYC 3 cut(s) 198, 1009, 1068
FaeI CATG 4 cut(s) 541, 742, 826, 917
FalI AAGNNNNNCTT 2 cut(s) 1052, 1084
FaqI GGGAC 3 cut(s) 10, 526, 812
FatI CATG 4 cut(s) 537, 738, 822, 913
FauI CCCGC 6 cut(s) 207, 258, 477, 818, 994, 1076
FblI GTMKAC 1 cut(s) 746
Fnu4HI GCNGC 8 cut(s) 120, 254, 263, 266, 276, 411, 450, 458
FokI GGATG 5 cut(s) 26, 630, 807, 980, 1046
FriOI GRGCYC 3 cut(s) 198, 1009, 1068
Fsp4HI GCNGC 8 cut(s) 120, 254, 263, 266, 276, 411, 450, 458
FspBI CTAG 4 cut(s) 317, 606, 1170, 1190
GlaI GCGC 1 cut(s) 203
GluI GCNGC 8 cut(s) 120, 254, 263, 266, 276, 411, 450, 458
GsaI CCCAGC 1 cut(s) 492
GsuI CTGGAG 2 cut(s) 254, 617
HaeIII GGCC 3 cut(s) 189, 249, 643
HapII CCGG 3 cut(s) 231, 1017, 1098
HgaI GACGC 2 cut(s) 338, 463
HhaI GCGC 1 cut(s) 204
Hin1I GRCGYC 1 cut(s) 330
Hin1II CATG 4 cut(s) 541, 742, 826, 917
Hin6I GCGC 1 cut(s) 202
HinP1I GCGC 1 cut(s) 202
HindIII AAGCTT 1 cut(s) 321
HinfI GANTC 6 cut(s) 141, 213, 292, 628, 1150, 1217
HpaII CCGG 3 cut(s) 231, 1017, 1098
HphI GGTGA 5 cut(s) 125, 220, 433, 454, 840
Hpy166II GTNNAC 1 cut(s) 747
Hpy188I TCNGA 7 cut(s) 328, 367, 390, 438, 526, 1092, 1222
Hpy188III TCNNGA 7 cut(s) 152, 208, 317, 776, 914, 1046, 1214
Hpy8I GTNNAC 1 cut(s) 747
Hpy99I CGWCG 4 cut(s) 173, 332, 442, 457
HpyAV CCTTC 4 cut(s) 239, 702, 848, 851
HpyCH4III ACNGT 3 cut(s) 442, 871, 907
HpyCH4V TGCA 5 cut(s) 379, 460, 559, 879, 922
HpyF10VI GCNNNNNNNGC 7 cut(s) 56, 80, 180, 259, 262, 329, 1013
HpyF3I CTNAG 3 cut(s) 351, 515, 1236
Hsp92I GRCGYC 1 cut(s) 330
Hsp92II CATG 4 cut(s) 541, 742, 826, 917
HspAI GCGC 1 cut(s) 202
Kzo9I GATC 6 cut(s) 130, 313, 583, 655, 767, 1036
LmnI GCTCC 8 cut(s) 201, 273, 309, 407, 598, 1004, 1012, 1063
Lsp1109I GCAGC 3 cut(s) 252, 265, 444
LweI GCATC 2 cut(s) 366, 1096
MaeI CTAG 4 cut(s) 317, 606, 1170, 1190
MaeIII GTNAC 2 cut(s) 421, 846
MalI GATC 6 cut(s) 132, 315, 585, 657, 769, 1038
MboI GATC 6 cut(s) 130, 313, 583, 655, 767, 1036
MboII GAAGA 3 cut(s) 797, 809, 1128
MflI RGATCY 1 cut(s) 583
MhlI GDGCHC 4 cut(s) 198, 603, 1009, 1068
MluCI AATT 5 cut(s) 160, 779, 835, 1053, 1225
MluI ACGCGT 1 cut(s) 381
MmeI TCCRAC 5 cut(s) 39, 345, 351, 385, 461
MseI TTAA 3 cut(s) 86, 510, 588
MslI CAYNNNNRTG 1 cut(s) 984
MspA1I CMGCKG 1 cut(s) 256
MspI CCGG 3 cut(s) 231, 1017, 1098
MspR9I CCNGG 5 cut(s) 272, 347, 596, 660, 1098
Mva1269I GAATGC 1 cut(s) 397
MvaI CCWGG 4 cut(s) 272, 347, 596, 660
MvnI CGCG 5 cut(s) 202, 218, 383, 428, 464
MwoI GCNNNNNNNGC 7 cut(s) 56, 80, 180, 259, 262, 329, 1013
NciI CCSGG 1 cut(s) 1098
NdeII GATC 6 cut(s) 130, 313, 583, 655, 767, 1036
NlaIII CATG 4 cut(s) 541, 742, 826, 917
NlaIV GGNNCC 2 cut(s) 235, 409
NmeAIII GCCGAG 2 cut(s) 358, 603
NmuCI GTSAC 2 cut(s) 421, 846
PaeR7I CTCGAG 1 cut(s) 191
PagI TCATGA 1 cut(s) 913
PcsI WCGNNNNNNNCGW 1 cut(s) 150
PctI GAATGC 1 cut(s) 397
PfeI GAWTC 6 cut(s) 141, 213, 292, 628, 1150, 1217
PfoI TCCNGGA 1 cut(s) 594
PinAI ACCGGT 1 cut(s) 230
PkrI GCNGC 8 cut(s) 121, 255, 264, 267, 277, 412, 451, 459
PshBI ATTAAT 1 cut(s) 510
Psp124BI GAGCTC 3 cut(s) 198, 1009, 1068
Psp6I CCWGG 4 cut(s) 270, 345, 594, 658
PspEI GGTNACC 1 cut(s) 421
PspFI CCCAGC 1 cut(s) 488
PspGI CCWGG 4 cut(s) 270, 345, 594, 658
PspN4I GGNNCC 2 cut(s) 235, 409
PspPI GGNCC 3 cut(s) 248, 434, 1019
PspXI VCTCGAGB 1 cut(s) 191
PstNI CAGNNNCTG 1 cut(s) 1160
PsuI RGATCY 1 cut(s) 583
PvuII CAGCTG 1 cut(s) 256
RsaI GTAC 4 cut(s) 221, 281, 668, 827
RsaNI GTAC 4 cut(s) 220, 280, 667, 826
RseI CAYNNNNRTG 1 cut(s) 984
SacI GAGCTC 3 cut(s) 198, 1009, 1068
SaqAI TTAA 3 cut(s) 86, 510, 588
SatI GCNGC 8 cut(s) 120, 254, 263, 266, 276, 411, 450, 458
Sau3AI GATC 6 cut(s) 130, 313, 583, 655, 767, 1036
Sau96I GGNCC 3 cut(s) 248, 434, 1019
ScrFI CCNGG 5 cut(s) 272, 347, 596, 660, 1098
SduI GDGCHC 4 cut(s) 198, 603, 1009, 1068
SfaNI GCATC 2 cut(s) 366, 1096
SfcI CTRYAG 1 cut(s) 747
Sfr274I CTCGAG 1 cut(s) 191
SinI GGWCC 2 cut(s) 434, 1019
SlaI CTCGAG 1 cut(s) 191
SmiMI CAYNNNNRTG 1 cut(s) 984
SmlI CTYRAG 1 cut(s) 191
SmoI CTYRAG 1 cut(s) 191
Sse9I AATT 5 cut(s) 160, 779, 835, 1053, 1225
SspI AATATT 1 cut(s) 613
SspMI CTAG 4 cut(s) 317, 606, 1170, 1190
SstI GAGCTC 3 cut(s) 198, 1009, 1068
StyD4I CCNGG 5 cut(s) 270, 345, 594, 658, 1096
TaaI ACNGT 3 cut(s) 442, 871, 907
TaqI TCGA 4 cut(s) 168, 192, 312, 1047
TasI AATT 5 cut(s) 160, 779, 835, 1053, 1225
TatI WGTACW 1 cut(s) 825
TauI GCSGC 5 cut(s) 122, 265, 278, 413, 452
TfiI GAWTC 6 cut(s) 141, 213, 292, 628, 1150, 1217
Tru1I TTAA 3 cut(s) 86, 510, 588
Tru9I TTAA 3 cut(s) 86, 510, 588
TscAI CASTG 5 cut(s) 523, 874, 978, 1161, 1247
TseFI GTSAC 2 cut(s) 421, 846
TseI GCWGC 3 cut(s) 253, 265, 457
Tsp45I GTSAC 2 cut(s) 421, 846
TspDTI ATGAA 4 cut(s) 17, 154, 558, 727
TspRI CASTG 5 cut(s) 523, 874, 978, 1161, 1247
VpaK11BI GGWCC 2 cut(s) 434, 1019
VspI ATTAAT 1 cut(s) 510
XapI RAATTY 2 cut(s) 779, 1225
XbaI TCTAGA 1 cut(s) 316
XhoI CTCGAG 1 cut(s) 191
XmiI GTMKAC 1 cut(s) 746
XspI CTAG 4 cut(s) 317, 606, 1170, 1190
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.