Rw3G012460

Core-2/I-Branching enzyme

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr3
OG0015805
Physical Location & Seq
Reverse (-)
12234738 .. 12236982
2245 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw3G012460.1

Sequence Viewer

Length: 1260 bp
ATGAAACGAACCAGCCACAATCAGCCTCAATGGCTCGTCATTCTAACCGCGACCTCCGCTCTCCTCCTCCTCGCTCTCCTCGCCCTCACCACCACCCACGCCAAGTATTCCTCCTCCGCCGTCCCCGACTTCACCTACGACCACCGCCGATTCCCTAATCCCGACCAGTATTTCGTCGACAGCAACGACGACGACGAGAACTACGGCCTAGGGCTCCCGAGATTCGCTTACCTGATTACCGGTTCTAAAGGTGACGGCCCTCAGCTGAAGCGGCTGCTTCAGGCGGCGTACCACCCGAGAAATTACTACTTGCTCCACCTCGATCTGGAAGCTTCCGACGCCGAGAGGCTCGAGCTCGCCAAGTACGTCAAGTCCGAGAGTGTGATGAACGCGTTTCGGAATGCGATGGTGGTTGGAACCGCGGATTTGGTGACCGCGAAAGGTCCGACGGTGTTGGCGGCGACGCTGCACGCGGTTGCGATTTTGCTCAAGCGGGCCGAGGATTGGGATTGGTTTATTAATCTCAGTGCTTCGGATTATCCGCTCATGTCCCAAGATGATTTGCTGCATATATTTTCATACTTGCCAAGGGACCTCAACTTCCTGGAGCACACTAGCAATATTGGGTGGAAAGAAAATCAAAGAGCGAGGCCTATTATAATTGATCCGGGTTTGTACCACACTAAGAAATCCGGTGTGTTTTGGGCTAAAGAGAGAAGGTCGCTGCCAGCTTCCTTCAAGTTATTCATGGGGTCTACAGGGGTGGTGCTGACAAGATCATTCCTTGAATTCTGTGTTTTGGGATGGGACAATCTTCCCCGCACTCTTCTCATGTATTATACCAATTTCCTGTCATCCACAGAGGGCTACTTCCACACTGTTGTCTGCAACCATAAGGATTACCAGAACACGACTGTGAATCATGACTTGCATTACATAAGGTGGGATAGTCCTCCAAAGCTAAATCCAGTTAATCTAACACTGGAACATTACAAAGACATCATCCAAGCGGGAGCTCCCTTCGCCAGTCCATTTGTGAGGGATGATCCGGTTCTTGACAAAATTGATAAGGAGCTCTTGAGGAGACCAAGCGGGCATGTTACACCCGGTGGTTGGTGTTTAGGAAGTACAGGTAAAGATAGATGTTCAGTTTATGGGAATCCAGTGGCTGTAAAACCTACTGTTGTTTCAAAGAGACTAGAGAAACTGATTGTGAAACTTCTCGATTCTGAAAATTTCAGGTCCAAGCAGTGTAAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0008194 GO:0015020 GO:0016740 GO:0016757 GO:0016758
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Branch

Protein Analysis

419

Amino Acids

47.57

Weight (kDa)

8.59

Isoelectric Point (pI)

40.92

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Branch PF02485 75 - 334 2.2e-71 Core-2/I-Branching enzyme
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015805)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G37585
fragaria_vesca FvH4_6g14020 FvH4_6g14020
malus_domestica MD12G1078600.v1.1
prunus_persica Prupe.6G253100_v2.0.a1 Prupe.6G253100_v2.0.a1
pyrus_communis pycom12g13490
rosa_multiflora Rmu_co8144344.1_g000001
rosa_roxburghii Rroxscaffold_6G00414080
rosa_rugosa Rorug03G0084200
rosa_samantha Rh3AG131800 Rh3BG152900 Rh3CG153100 Rh3DG154000
rosa_wichuraiana Rw3G012460

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 659
AccBSI CCGCTC 2 cut(s) 59, 544
AccI GTMKAC 2 cut(s) 177, 755
AccII CGCG 5 cut(s) 50, 392, 422, 437, 473
AclWI GGATC 2 cut(s) 659, 1040
AcsI RAATTY 2 cut(s) 788, 1234
AcuI CTGAAG 2 cut(s) 263, 287
AcyI GRCGYC 1 cut(s) 339
AdeI CACNNNGTG 1 cut(s) 1109
AfaI GTAC 4 cut(s) 290, 365, 677, 1129
AfiI CCNNNNNNNGG 3 cut(s) 325, 504, 1113
AflIII ACRYGT 1 cut(s) 390
AgeI ACCGGT 1 cut(s) 239
AgsI TTSAA 3 cut(s) 739, 788, 1191
AjnI CCWGG 1 cut(s) 603
AleI CACNNNNGTG 1 cut(s) 914
AluBI AGCT 7 cut(s) 265, 332, 355, 731, 961, 1016, 1075
AluI AGCT 7 cut(s) 265, 332, 355, 731, 961, 1016, 1075
Alw21I GWGCWC 4 cut(s) 357, 612, 1018, 1077
Alw26I GTCTC 2 cut(s) 1078, 1189
AlwI GGATC 2 cut(s) 659, 1040
AlwNI CAGNNNCTG 1 cut(s) 1169
Ama87I CYCGRG 3 cut(s) 217, 295, 350
AoxI GGCC 4 cut(s) 205, 256, 495, 650
ApeKI GCWGC 4 cut(s) 274, 466, 565, 724
ApoI RAATTY 2 cut(s) 788, 1234
ArsI GACNNNNNNTTYG 2 cut(s) 155, 187
AseI ATTAAT 1 cut(s) 519
AsiGI ACCGGT 1 cut(s) 239
AspA2I CCTAGG 1 cut(s) 208
AspS9I GGNCC 5 cut(s) 257, 443, 495, 592, 1242
AsuC2I CCSGG 2 cut(s) 669, 1107
AsuHPI GGTGA 4 cut(s) 79, 124, 263, 442
AvaI CYCGRG 3 cut(s) 217, 295, 350
AvaII GGWCC 3 cut(s) 443, 592, 1242
AvrII CCTAGG 1 cut(s) 208
BanII GRGCYC 4 cut(s) 216, 357, 1018, 1077
Bbv12I GWGCWC 4 cut(s) 357, 612, 1018, 1077
BbvCI CCTCAGC 1 cut(s) 261
BbvI GCAGC 4 cut(s) 261, 453, 552, 711
BccI CCATC 2 cut(s) 400, 798
BceAI ACGGC 3 cut(s) 104, 220, 271
BciT130I CCWGG 1 cut(s) 605
BcnI CCSGG 2 cut(s) 669, 1107
BcoDI GTCTC 2 cut(s) 1078, 1189
BfaI CTAG 3 cut(s) 209, 615, 1199
BfmI CTRYAG 1 cut(s) 756
BglI GCCNNNNNGGC 1 cut(s) 31
BisI GCNGC 7 cut(s) 272, 275, 285, 459, 467, 566, 725
BlnI CCTAGG 1 cut(s) 208
BlsI GCNGC 7 cut(s) 273, 276, 286, 460, 468, 567, 726
Bme1390I CCNGG 3 cut(s) 605, 669, 1107
Bme18I GGWCC 3 cut(s) 443, 592, 1242
BmeT110I CYCGRG 3 cut(s) 217, 295, 350
BmgT120I GGNCC 5 cut(s) 257, 443, 495, 592, 1242
BmiI GGNNCC 3 cut(s) 215, 418, 593
BmrFI CCNGG 3 cut(s) 605, 669, 1107
BpmI CTGGAG 1 cut(s) 626
Bpu10I CCTNAGC 1 cut(s) 261
BpuEI CTTGAG 2 cut(s) 473, 1099
BpuMI CCSGG 2 cut(s) 669, 1107
BsaHI GRCGYC 1 cut(s) 339
BsaI GGTCTC 1 cut(s) 1078
BsaJI CCNNGG 4 cut(s) 208, 420, 498, 587
BsaWI WCCGGW 3 cut(s) 239, 692, 1048
Bsc4I CCNNNNNNNGG 3 cut(s) 325, 504, 1113
Bse118I RCCGGY 1 cut(s) 239
Bse1I ACTGG 5 cut(s) 166, 968, 987, 1026, 1163
BseBI CCWGG 1 cut(s) 605
BseDI CCNNGG 4 cut(s) 208, 420, 498, 587
BseGI GGATG 4 cut(s) 809, 854, 1002, 1048
BseLI CCNNNNNNNGG 3 cut(s) 325, 504, 1113
BseMII CTCAG 2 cut(s) 275, 538
BseNI ACTGG 5 cut(s) 166, 968, 987, 1026, 1163
BseRI GAGGAG 6 cut(s) 53, 56, 59, 68, 103, 1096
BseXI GCAGC 4 cut(s) 261, 453, 552, 711
BsgI GTGCAG 1 cut(s) 452
Bsh1236I CGCG 5 cut(s) 50, 392, 422, 437, 473
BshFI GGCC 4 cut(s) 207, 258, 497, 652
BshTI ACCGGT 1 cut(s) 239
BsiHKAI GWGCWC 4 cut(s) 357, 612, 1018, 1077
BsiHKCI CYCGRG 3 cut(s) 217, 295, 350
BsiSI CCGG 5 cut(s) 240, 668, 693, 1049, 1107
BslFI GGGAC 4 cut(s) 107, 535, 605, 821
BslI CCNNNNNNNGG 3 cut(s) 325, 504, 1113
BsmAI GTCTC 2 cut(s) 1078, 1189
BsmFI GGGAC 4 cut(s) 107, 535, 605, 821
BsmI GAATGC 1 cut(s) 406
BsnI GGCC 4 cut(s) 207, 258, 497, 652
Bso31I GGTCTC 1 cut(s) 1078
BsoBI CYCGRG 3 cut(s) 217, 295, 350
Bsp1286I GDGCHC 5 cut(s) 216, 357, 612, 1018, 1077
Bsp143I GATC 4 cut(s) 322, 664, 776, 1045
BspANI GGCC 4 cut(s) 207, 258, 497, 652
BspCNI CTCAG 2 cut(s) 274, 537
BspFNI CGCG 5 cut(s) 50, 392, 422, 437, 473
BspHI TCATGA 1 cut(s) 922
BspLI GGNNCC 3 cut(s) 215, 418, 593
BspPI GGATC 2 cut(s) 659, 1040
BspTNI GGTCTC 1 cut(s) 1078
BsrBI CCGCTC 2 cut(s) 59, 544
BsrFI RCCGGY 1 cut(s) 239
BsrI ACTGG 5 cut(s) 166, 968, 987, 1026, 1163
BssAI RCCGGY 1 cut(s) 239
BssECI CCNNGG 4 cut(s) 208, 420, 498, 587
BssMI GATC 4 cut(s) 322, 664, 776, 1045
BssNI GRCGYC 1 cut(s) 339
BssT1I CCWWGG 2 cut(s) 208, 587
Bst2UI CCWGG 1 cut(s) 605
Bst4CI ACNGT 4 cut(s) 451, 880, 916, 1183
Bst6I CTCTTC 1 cut(s) 831
BstACI GRCGYC 1 cut(s) 339
BstC8I GCNNGC 5 cut(s) 357, 471, 495, 729, 1094
BstDEI CTNAG 3 cut(s) 261, 524, 684
BstDSI CCRYGG 1 cut(s) 420
BstEII GGTNACC 1 cut(s) 430
BstF5I GGATG 4 cut(s) 809, 854, 1002, 1048
BstFNI CGCG 5 cut(s) 50, 392, 422, 437, 473
BstKTI GATC 4 cut(s) 325, 667, 779, 1048
BstMAI GTCTC 2 cut(s) 1078, 1189
BstMBI GATC 4 cut(s) 322, 664, 776, 1045
BstMWI GCNNNNNNNGC 6 cut(s) 31, 56, 80, 271, 338, 1022
BstNI CCWGG 1 cut(s) 605
BstNSI RCATGY 1 cut(s) 1100
BstPI GGTNACC 1 cut(s) 430
BstSCI CCNGG 3 cut(s) 603, 667, 1105
BstSFI CTRYAG 1 cut(s) 756
BstUI CGCG 5 cut(s) 50, 392, 422, 437, 473
BstV1I GCAGC 4 cut(s) 261, 453, 552, 711
BsuRI GGCC 4 cut(s) 207, 258, 497, 652
BtgI CCRYGG 1 cut(s) 420
BtgZI GCGATG 1 cut(s) 419
BtsCI GGATG 4 cut(s) 809, 854, 1002, 1048
BtsI GCAGTG 1 cut(s) 1256
BtsIMutI CAGTG 5 cut(s) 532, 876, 980, 1170, 1256
Cac8I GCNNGC 5 cut(s) 357, 471, 495, 729, 1094
CaiI CAGNNNCTG 1 cut(s) 1169
CciI TCATGA 1 cut(s) 922
Cfr10I RCCGGY 1 cut(s) 239
Cfr13I GGNCC 5 cut(s) 257, 443, 495, 592, 1242
Cfr42I CCGCGG 1 cut(s) 423
CseI GACGC 2 cut(s) 347, 472
Csp6I GTAC 4 cut(s) 289, 364, 676, 1128
CspAI ACCGGT 1 cut(s) 239
CviAII CATG 5 cut(s) 547, 748, 832, 923, 1097
CviQI GTAC 4 cut(s) 289, 364, 676, 1128
DdeI CTNAG 3 cut(s) 261, 524, 684
DpnI GATC 4 cut(s) 324, 666, 778, 1047
DpnII GATC 4 cut(s) 322, 664, 776, 1045
DraIII CACNNNGTG 1 cut(s) 1109
Eam1104I CTCTTC 1 cut(s) 831
EarI CTCTTC 1 cut(s) 831
EciI GGCGGA 1 cut(s) 106
Ecl136II GAGCTC 3 cut(s) 355, 1016, 1075
Eco130I CCWWGG 2 cut(s) 208, 587
Eco147I AGGCCT 1 cut(s) 652
Eco24I GRGCYC 4 cut(s) 216, 357, 1018, 1077
Eco31I GGTCTC 1 cut(s) 1078
Eco47I GGWCC 3 cut(s) 443, 592, 1242
Eco53kI GAGCTC 3 cut(s) 355, 1016, 1075
Eco57I CTGAAG 2 cut(s) 263, 287
Eco88I CYCGRG 3 cut(s) 217, 295, 350
Eco91I GGTNACC 1 cut(s) 430
EcoICRI GAGCTC 3 cut(s) 355, 1016, 1075
EcoO109I RGGNCCY 1 cut(s) 592
EcoO65I GGTNACC 1 cut(s) 430
EcoRI GAATTC 1 cut(s) 788
EcoRII CCWGG 1 cut(s) 603
EcoT14I CCWWGG 2 cut(s) 208, 587
EcoT38I GRGCYC 4 cut(s) 216, 357, 1018, 1077
ErhI CCWWGG 2 cut(s) 208, 587
FaeI CATG 5 cut(s) 550, 751, 835, 926, 1100
FalI AAGNNNNNCTT 2 cut(s) 1061, 1093
FaqI GGGAC 4 cut(s) 107, 535, 605, 821
FatI CATG 5 cut(s) 546, 747, 831, 922, 1096
FauI CCCGC 4 cut(s) 486, 827, 1003, 1085
FblI GTMKAC 2 cut(s) 177, 755
Fnu4HI GCNGC 7 cut(s) 272, 275, 285, 459, 467, 566, 725
FokI GGATG 4 cut(s) 816, 841, 989, 1055
FriOI GRGCYC 4 cut(s) 216, 357, 1018, 1077
Fsp4HI GCNGC 7 cut(s) 272, 275, 285, 459, 467, 566, 725
FspBI CTAG 3 cut(s) 209, 615, 1199
GluI GCNGC 7 cut(s) 272, 275, 285, 459, 467, 566, 725
GsuI CTGGAG 1 cut(s) 626
HaeIII GGCC 4 cut(s) 207, 258, 497, 652
HapII CCGG 5 cut(s) 240, 668, 693, 1049, 1107
HgaI GACGC 2 cut(s) 347, 472
Hin1I GRCGYC 1 cut(s) 339
Hin1II CATG 5 cut(s) 550, 751, 835, 926, 1100
HincII GTYRAC 1 cut(s) 178
HindII GTYRAC 1 cut(s) 178
HindIII AAGCTT 1 cut(s) 330
HinfI GANTC 5 cut(s) 150, 222, 919, 1159, 1226
HpaII CCGG 5 cut(s) 240, 668, 693, 1049, 1107
HphI GGTGA 4 cut(s) 79, 124, 263, 442
Hpy166II GTNNAC 2 cut(s) 178, 756
Hpy188I TCNGA 6 cut(s) 337, 376, 399, 447, 535, 1231
Hpy188III TCNNGA 7 cut(s) 161, 217, 326, 923, 1055, 1078, 1223
Hpy8I GTNNAC 2 cut(s) 178, 756
Hpy99I CGWCG 7 cut(s) 179, 191, 194, 197, 341, 451, 466
HpyAV CCTTC 3 cut(s) 711, 745, 1030
HpyCH4III ACNGT 4 cut(s) 451, 880, 916, 1183
HpyCH4IV ACGT 1 cut(s) 366
HpyCH4V TGCA 4 cut(s) 469, 568, 888, 931
HpyF10VI GCNNNNNNNGC 6 cut(s) 31, 56, 80, 271, 338, 1022
HpyF3I CTNAG 3 cut(s) 261, 524, 684
HpySE526I ACGT 1 cut(s) 366
Hsp92I GRCGYC 1 cut(s) 339
Hsp92II CATG 5 cut(s) 550, 751, 835, 926, 1100
KspI CCGCGG 1 cut(s) 423
Kzo9I GATC 4 cut(s) 322, 664, 776, 1045
LmnI GCTCC 6 cut(s) 219, 318, 607, 1013, 1021, 1072
Lsp1109I GCAGC 4 cut(s) 261, 453, 552, 711
MaeI CTAG 3 cut(s) 209, 615, 1199
MaeII ACGT 1 cut(s) 366
MaeIII GTNAC 3 cut(s) 251, 430, 1099
MalI GATC 4 cut(s) 324, 666, 778, 1047
MbiI CCGCTC 2 cut(s) 59, 544
MboI GATC 4 cut(s) 322, 664, 776, 1045
MboII GAAGA 2 cut(s) 806, 818
MhlI GDGCHC 5 cut(s) 216, 357, 612, 1018, 1077
MluCI AATT 6 cut(s) 301, 660, 788, 844, 1062, 1234
MluI ACGCGT 1 cut(s) 390
MmeI TCCRAC 3 cut(s) 360, 394, 470
MseI TTAA 2 cut(s) 519, 972
MslI CAYNNNNRTG 1 cut(s) 914
MspA1I CMGCKG 2 cut(s) 265, 422
MspI CCGG 5 cut(s) 240, 668, 693, 1049, 1107
MspR9I CCNGG 3 cut(s) 605, 669, 1107
Mva1269I GAATGC 1 cut(s) 406
MvaI CCWGG 1 cut(s) 605
MvnI CGCG 5 cut(s) 50, 392, 422, 437, 473
MwoI GCNNNNNNNGC 6 cut(s) 31, 56, 80, 271, 338, 1022
NciI CCSGG 2 cut(s) 669, 1107
NdeII GATC 4 cut(s) 322, 664, 776, 1045
NlaIII CATG 5 cut(s) 550, 751, 835, 926, 1100
NlaIV GGNNCC 3 cut(s) 215, 418, 593
NmeAIII GCCGAG 2 cut(s) 367, 523
NmuCI GTSAC 2 cut(s) 251, 430
NspI RCATGY 1 cut(s) 1100
OliI CACNNNNGTG 1 cut(s) 914
PaeR7I CTCGAG 1 cut(s) 350
PagI TCATGA 1 cut(s) 922
PceI AGGCCT 1 cut(s) 652
PcsI WCGNNNNNNNCGW 4 cut(s) 183, 192, 363, 372
PctI GAATGC 1 cut(s) 406
PfeI GAWTC 5 cut(s) 150, 222, 919, 1159, 1226
PfoI TCCNGGA 1 cut(s) 603
PinAI ACCGGT 1 cut(s) 239
PkrI GCNGC 7 cut(s) 273, 276, 286, 460, 468, 567, 726
PpuMI RGGWCCY 1 cut(s) 592
PshBI ATTAAT 1 cut(s) 519
PsiI TTATAA 1 cut(s) 659
Psp124BI GAGCTC 3 cut(s) 357, 1018, 1077
Psp5II RGGWCCY 1 cut(s) 592
Psp6I CCWGG 1 cut(s) 603
PspEI GGTNACC 1 cut(s) 430
PspGI CCWGG 1 cut(s) 603
PspN4I GGNNCC 3 cut(s) 215, 418, 593
PspPI GGNCC 5 cut(s) 257, 443, 495, 592, 1242
PspPPI RGGWCCY 1 cut(s) 592
PspXI VCTCGAGB 1 cut(s) 350
PstNI CAGNNNCTG 1 cut(s) 1169
PvuII CAGCTG 1 cut(s) 265
RsaI GTAC 4 cut(s) 290, 365, 677, 1129
RsaNI GTAC 4 cut(s) 289, 364, 676, 1128
RseI CAYNNNNRTG 1 cut(s) 914
SacI GAGCTC 3 cut(s) 357, 1018, 1077
SacII CCGCGG 1 cut(s) 423
SalI GTCGAC 1 cut(s) 176
SaqAI TTAA 2 cut(s) 519, 972
SatI GCNGC 7 cut(s) 272, 275, 285, 459, 467, 566, 725
Sau3AI GATC 4 cut(s) 322, 664, 776, 1045
Sau96I GGNCC 5 cut(s) 257, 443, 495, 592, 1242
ScrFI CCNGG 3 cut(s) 605, 669, 1107
SduI GDGCHC 5 cut(s) 216, 357, 612, 1018, 1077
SfcI CTRYAG 1 cut(s) 756
Sfr274I CTCGAG 1 cut(s) 350
Sfr303I CCGCGG 1 cut(s) 423
SgrBI CCGCGG 1 cut(s) 423
SinI GGWCC 3 cut(s) 443, 592, 1242
SlaI CTCGAG 1 cut(s) 350
SmiMI CAYNNNNRTG 1 cut(s) 914
SmlI CTYRAG 3 cut(s) 350, 488, 1078
SmoI CTYRAG 3 cut(s) 350, 488, 1078
Sse9I AATT 6 cut(s) 301, 660, 788, 844, 1062, 1234
SseBI AGGCCT 1 cut(s) 652
SspI AATATT 1 cut(s) 622
SspMI CTAG 3 cut(s) 209, 615, 1199
SstI GAGCTC 3 cut(s) 357, 1018, 1077
StuI AGGCCT 1 cut(s) 652
StyD4I CCNGG 3 cut(s) 603, 667, 1105
StyI CCWWGG 2 cut(s) 208, 587
TaaI ACNGT 4 cut(s) 451, 880, 916, 1183
TaiI ACGT 1 cut(s) 369
TaqI TCGA 4 cut(s) 177, 321, 351, 1224
TasI AATT 6 cut(s) 301, 660, 788, 844, 1062, 1234
TatI WGTACW 1 cut(s) 1127
TauI GCSGC 3 cut(s) 274, 287, 461
TfiI GAWTC 5 cut(s) 150, 222, 919, 1159, 1226
Tru1I TTAA 2 cut(s) 519, 972
Tru9I TTAA 2 cut(s) 519, 972
TscAI CASTG 5 cut(s) 532, 883, 987, 1170, 1256
TseFI GTSAC 2 cut(s) 251, 430
TseI GCWGC 4 cut(s) 274, 466, 565, 724
Tsp45I GTSAC 2 cut(s) 251, 430
TspDTI ATGAA 4 cut(s) 17, 401, 567, 736
TspRI CASTG 5 cut(s) 532, 883, 987, 1170, 1256
VpaK11BI GGWCC 3 cut(s) 443, 592, 1242
VspI ATTAAT 1 cut(s) 519
XapI RAATTY 2 cut(s) 788, 1234
XceI RCATGY 1 cut(s) 1100
XhoI CTCGAG 1 cut(s) 350
XmaJI CCTAGG 1 cut(s) 208
XmiI GTMKAC 2 cut(s) 177, 755
XspI CTAG 3 cut(s) 209, 615, 1199
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.