MD12G1078600.v1.1

Core-2/I-Branching enzyme

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0015805
Physical Location & Seq
Reverse (-)
9575170 .. 9577803
2634 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1078600.v1.1.491

Sequence Viewer

Length: 1272 bp
ATGAAAAGAGCTCGCTCTCAATGGCTCATGATTTTAACCGCCACCTCCCTCCTCTTCCTCTTCCTCCTCCTCACCCTTACTCTCGCCGGCCACGGTAAATACTCCTCCTCCGCCGCCGCCGACTTTTATTACGAGCAACGCGAGTTCACCACCCTCGACCGGAATTTCGGAGACACGACGGAAGGCACCGACAACGAAAACAGCCACAGGTTAGGGATTCCGAAACTCCCGAGATTCGCCTACCTCATATCGGGCTCCAAGGGCGACGGCCCCCAGCTGCGGCGGCTGCTTCAGGCGGTTTACCACCCCAGAAATTACTATTTACTGCACCTCGATCTCGAAGCTTCGGATGCTGAGCGGCTCGAGCTGGCGAAGTATGTGAAGTCGGAGAGCGCGATTCGGGAGTTTCGGAACGCGATGGTGATCGGGAACGCCGATTTGGTGACCGCCAAGGGACCCACCATGACTGCCGCCACGCTCCACGCCATTGCGATTTTGCTGAAGCGGGCGAAGGATTGGGATTGGTTTATCAATCTCGGCGCCTCGGATTATCCCCTCATGTCCCAAGACGATCTGTTGCATATATTTTCTTTCTTGCCAAGGGACCTCAATTTCTTGGAGCACACAAGTAACATTGGGTGGAAAGAAAATCAAAGAGCAAGGCCTATTATTATTGATCCTGGTTTGTACCACTCTAAGAAATCCGCTGTGTTTTGGGCGAAAGAGCGAAGGTCAATACCCGCTTCGTTTAAGTTATACATGGGGTCGACGTGGGTGGTGCTGACCAAATCATTTCTCGAATTCTGTGTTTGGGGATGGGACAATCTACCCCGTACTCTTCTCATGTACTACACCAATTTCTTGTCATCCCCAGAGGGCTACTTCCACACTGTTGCGTGCAACCACAAGGACTACCAGAACACGACCGTGAACCATAACTTGCATTATATAAGGTGGGATAGCCCTCCGAAGGCAAATCCAATCAATCTAACGCTGGAACACTACAACGACATGGTCCAAAGTGGAGCTCCGTTTGCGGGTACATTTTCAAGGGATGATCCAGTTCTCAACAAAATCGATAAGGAGCTCCTGAGGAGACCAAGCGGCCACTTCACTCCCGGTGGTTGGTGCTTGGGAAATTTAGGTGAGAATCCTTGTTTAGTGTATGGGAATTCAAATGCTGTAAAACCAACTGTAGTTTCAAAGAGACTAGAGAAACTCATTGTGAACCTTCTGGATTCCGAAAATTTCAGGCCTAAGCAGTGTAAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0008194 GO:0015020 GO:0016740 GO:0016757 GO:0016758
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Branch

Protein Analysis

424

Amino Acids

48.32

Weight (kDa)

8.97

Isoelectric Point (pI)

41.24

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Branch PF02485 79 - 338 2.5e-72 Core-2/I-Branching enzyme
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015805)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G37585
fragaria_vesca FvH4_6g14020 FvH4_6g14020
malus_domestica MD12G1078600.v1.1
prunus_persica Prupe.6G253100_v2.0.a1 Prupe.6G253100_v2.0.a1
pyrus_communis pycom12g13490
rosa_multiflora Rmu_co8144344.1_g000001
rosa_roxburghii Rroxscaffold_6G00414080
rosa_rugosa Rorug03G0084200
rosa_samantha Rh3AG131800 Rh3BG152900 Rh3CG153100 Rh3DG154000
rosa_wichuraiana Rw3G012460

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 185, 539
AccBSI CCGCTC 1 cut(s) 358
AccI GTMKAC 1 cut(s) 767
AccII CGCG 3 cut(s) 141, 395, 416
AclWI GGATC 2 cut(s) 671, 1052
AcoI YGGCCR 2 cut(s) 88, 1105
AcsI RAATTY 5 cut(s) 163, 800, 1138, 1171, 1246
AcuI CTGAAG 2 cut(s) 275, 521
AcyI GRCGYC 1 cut(s) 540
AfaI GTAC 4 cut(s) 689, 835, 848, 1042
AfiI CCNNNNNNNGG 6 cut(s) 159, 250, 279, 970, 1037, 1125
AgsI TTSAA 3 cut(s) 1050, 1176, 1203
AjiI CACGTC 1 cut(s) 771
AjnI CCWGG 1 cut(s) 679
AjuI GAANNNNNNNTTGG 4 cut(s) 422, 454, 1183, 1215
AleI CACNNNNGTG 1 cut(s) 926
AluBI AGCT 6 cut(s) 11, 277, 344, 367, 1028, 1087
AluI AGCT 6 cut(s) 11, 277, 344, 367, 1028, 1087
Alw21I GWGCWC 4 cut(s) 13, 624, 1030, 1089
Alw26I GTCTC 3 cut(s) 165, 1090, 1201
AlwI GGATC 2 cut(s) 671, 1052
Ama87I CYCGRG 2 cut(s) 229, 362
AoxI GGCC 5 cut(s) 88, 268, 662, 1105, 1253
ApeKI GCWGC 2 cut(s) 277, 286
ApoI RAATTY 5 cut(s) 163, 800, 1138, 1171, 1246
ArsI GACNNNNNNTTYG 2 cut(s) 149, 181
AspLEI GCGC 2 cut(s) 395, 542
AspS9I GGNCC 4 cut(s) 269, 455, 604, 1015
AsuC2I CCSGG 1 cut(s) 1119
AsuHPI GGTGA 5 cut(s) 64, 139, 433, 454, 1157
AvaI CYCGRG 2 cut(s) 229, 362
AvaII GGWCC 3 cut(s) 455, 604, 1015
AxyI CCTNAGG 1 cut(s) 1091
BanI GGYRCC 2 cut(s) 185, 539
BanII GRGCYC 4 cut(s) 13, 257, 1030, 1089
Bbv12I GWGCWC 4 cut(s) 13, 624, 1030, 1089
BbvI GCAGC 2 cut(s) 264, 273
BccI CCATC 2 cut(s) 412, 810
BceAI ACGGC 1 cut(s) 283
BciT130I CCWGG 1 cut(s) 681
BcnI CCSGG 1 cut(s) 1119
BcoDI GTCTC 3 cut(s) 165, 1090, 1201
BfaI CTAG 1 cut(s) 1211
BfmI CTRYAG 1 cut(s) 1194
BfoI RGCGCY 1 cut(s) 543
BisI GCNGC 9 cut(s) 114, 117, 278, 281, 284, 287, 359, 471, 1105
BlpI GCTNAGC 1 cut(s) 354
BlsI GCNGC 9 cut(s) 115, 118, 279, 282, 285, 288, 360, 472, 1106
Bme1390I CCNGG 2 cut(s) 681, 1119
Bme18I GGWCC 3 cut(s) 455, 604, 1015
BmeT110I CYCGRG 2 cut(s) 229, 362
BmgBI CACGTC 1 cut(s) 771
BmgT120I GGNCC 4 cut(s) 269, 455, 604, 1015
BmiI GGNNCC 7 cut(s) 187, 256, 271, 456, 457, 541, 605
BmrFI CCNGG 2 cut(s) 681, 1119
BmsI GCATC 1 cut(s) 340
Bpu10I CCTNAGC 1 cut(s) 1257
Bpu1102I GCTNAGC 1 cut(s) 354
BpuMI CCSGG 1 cut(s) 1119
Bsa29I ATCGAT 1 cut(s) 1077
BsaBI GATNNNNATC 1 cut(s) 422
BsaHI GRCGYC 1 cut(s) 540
BsaI GGTCTC 1 cut(s) 1090
BsaJI CCNNGG 5 cut(s) 91, 258, 450, 543, 599
BsaWI WCCGGW 1 cut(s) 159
BsaXI ACNNNNNCTCC 2 cut(s) 92, 122
Bsc4I CCNNNNNNNGG 6 cut(s) 159, 250, 279, 970, 1037, 1125
Bse118I RCCGGY 1 cut(s) 86
Bse1I ACTGG 1 cut(s) 1061
Bse21I CCTNAGG 1 cut(s) 1091
Bse3DI GCAATG 1 cut(s) 486
Bse8I GATNNNNATC 1 cut(s) 422
BseBI CCWGG 1 cut(s) 681
BseCI ATCGAT 1 cut(s) 1077
BseDI CCNNGG 5 cut(s) 91, 258, 450, 543, 599
BseGI GGATG 4 cut(s) 355, 821, 866, 1060
BseJI GATNNNNATC 1 cut(s) 422
BseLI CCNNNNNNNGG 6 cut(s) 159, 250, 279, 970, 1037, 1125
BseMI GCAATG 1 cut(s) 486
BseMII CTCAG 2 cut(s) 345, 1082
BseNI ACTGG 1 cut(s) 1061
BseRI GAGGAG 6 cut(s) 41, 56, 59, 94, 97, 1108
BseXI GCAGC 2 cut(s) 264, 273
BseYI CCCAGC 1 cut(s) 273
BsgI GTGCAG 1 cut(s) 311
Bsh1236I CGCG 3 cut(s) 141, 395, 416
Bsh1285I CGRYCG 2 cut(s) 160, 927
BshFI GGCC 5 cut(s) 90, 270, 664, 1107, 1255
BshNI GGYRCC 2 cut(s) 185, 539
BshVI ATCGAT 1 cut(s) 1077
BsiEI CGRYCG 2 cut(s) 160, 927
BsiHKAI GWGCWC 4 cut(s) 13, 624, 1030, 1089
BsiHKCI CYCGRG 2 cut(s) 229, 362
BsiSI CCGG 3 cut(s) 87, 160, 1119
BslFI GGGAC 4 cut(s) 468, 547, 617, 833
BslI CCNNNNNNNGG 6 cut(s) 159, 250, 279, 970, 1037, 1125
BsmAI GTCTC 3 cut(s) 165, 1090, 1201
BsmFI GGGAC 4 cut(s) 468, 547, 617, 833
BsnI GGCC 5 cut(s) 90, 270, 664, 1107, 1255
Bso31I GGTCTC 1 cut(s) 1090
BsoBI CYCGRG 2 cut(s) 229, 362
Bsp1286I GDGCHC 5 cut(s) 13, 257, 624, 1030, 1089
Bsp143I GATC 5 cut(s) 334, 423, 571, 676, 1057
Bsp1720I GCTNAGC 1 cut(s) 354
BspANI GGCC 5 cut(s) 90, 270, 664, 1107, 1255
BspCNI CTCAG 2 cut(s) 346, 1083
BspDI ATCGAT 1 cut(s) 1077
BspFNI CGCG 3 cut(s) 141, 395, 416
BspHI TCATGA 1 cut(s) 27
BspLI GGNNCC 7 cut(s) 187, 256, 271, 456, 457, 541, 605
BspPI GGATC 2 cut(s) 671, 1052
BspT107I GGYRCC 2 cut(s) 185, 539
BspTNI GGTCTC 1 cut(s) 1090
BsrBI CCGCTC 1 cut(s) 358
BsrDI GCAATG 1 cut(s) 486
BsrFI RCCGGY 1 cut(s) 86
BsrI ACTGG 1 cut(s) 1061
BssAI RCCGGY 1 cut(s) 86
BssECI CCNNGG 5 cut(s) 91, 258, 450, 543, 599
BssMI GATC 5 cut(s) 334, 423, 571, 676, 1057
BssNI GRCGYC 1 cut(s) 540
BssT1I CCWWGG 3 cut(s) 258, 450, 599
Bst2UI CCWGG 1 cut(s) 681
Bst4CI ACNGT 4 cut(s) 95, 892, 928, 1195
Bst6I CTCTTC 3 cut(s) 59, 65, 843
BstACI GRCGYC 1 cut(s) 540
BstC8I GCNNGC 5 cut(s) 13, 88, 369, 507, 898
BstDEI CTNAG 4 cut(s) 354, 696, 1091, 1257
BstDSI CCRYGG 1 cut(s) 91
BstEII GGTNACC 1 cut(s) 442
BstF5I GGATG 4 cut(s) 355, 821, 866, 1060
BstFNI CGCG 3 cut(s) 141, 395, 416
BstH2I RGCGCY 1 cut(s) 543
BstHHI GCGC 2 cut(s) 395, 542
BstKTI GATC 5 cut(s) 337, 426, 574, 679, 1060
BstMAI GTCTC 3 cut(s) 165, 1090, 1201
BstMBI GATC 5 cut(s) 334, 423, 571, 676, 1057
BstMCI CGRYCG 2 cut(s) 160, 927
BstMWI GCNNNNNNNGC 6 cut(s) 261, 283, 286, 350, 364, 1034
BstNI CCWGG 1 cut(s) 681
BstPI GGTNACC 1 cut(s) 442
BstSCI CCNGG 2 cut(s) 679, 1117
BstSFI CTRYAG 1 cut(s) 1194
BstUI CGCG 3 cut(s) 141, 395, 416
BstV1I GCAGC 2 cut(s) 264, 273
Bsu15I ATCGAT 1 cut(s) 1077
Bsu36I CCTNAGG 1 cut(s) 1091
BsuRI GGCC 5 cut(s) 90, 270, 664, 1107, 1255
BsuTUI ATCGAT 1 cut(s) 1077
BtgI CCRYGG 1 cut(s) 91
BtgZI GCGATG 1 cut(s) 431
BtrI CACGTC 1 cut(s) 771
BtsCI GGATG 4 cut(s) 355, 821, 866, 1060
BtsI GCAGTG 1 cut(s) 1268
BtsIMutI CAGTG 2 cut(s) 888, 1268
Cac8I GCNNGC 5 cut(s) 13, 88, 369, 507, 898
CciI TCATGA 1 cut(s) 27
CfoI GCGC 2 cut(s) 395, 542
Cfr10I RCCGGY 1 cut(s) 86
Cfr13I GGNCC 4 cut(s) 269, 455, 604, 1015
ClaI ATCGAT 1 cut(s) 1077
Csp6I GTAC 4 cut(s) 688, 834, 847, 1041
CviAII CATG 6 cut(s) 28, 463, 559, 760, 844, 1012
CviQI GTAC 4 cut(s) 688, 834, 847, 1041
DdeI CTNAG 4 cut(s) 354, 696, 1091, 1257
DinI GGCGCC 1 cut(s) 541
DpnI GATC 5 cut(s) 336, 425, 573, 678, 1059
DpnII GATC 5 cut(s) 334, 423, 571, 676, 1057
EaeI YGGCCR 2 cut(s) 88, 1105
Eam1104I CTCTTC 3 cut(s) 59, 65, 843
EarI CTCTTC 3 cut(s) 59, 65, 843
EciI GGCGGA 1 cut(s) 100
Ecl136II GAGCTC 3 cut(s) 11, 1028, 1087
Eco130I CCWWGG 3 cut(s) 258, 450, 599
Eco147I AGGCCT 2 cut(s) 664, 1255
Eco24I GRGCYC 4 cut(s) 13, 257, 1030, 1089
Eco31I GGTCTC 1 cut(s) 1090
Eco47I GGWCC 3 cut(s) 455, 604, 1015
Eco53kI GAGCTC 3 cut(s) 11, 1028, 1087
Eco57I CTGAAG 2 cut(s) 275, 521
Eco81I CCTNAGG 1 cut(s) 1091
Eco88I CYCGRG 2 cut(s) 229, 362
Eco91I GGTNACC 1 cut(s) 442
EcoICRI GAGCTC 3 cut(s) 11, 1028, 1087
EcoO109I RGGNCCY 2 cut(s) 455, 604
EcoO65I GGTNACC 1 cut(s) 442
EcoRI GAATTC 2 cut(s) 800, 1171
EcoRII CCWGG 1 cut(s) 679
EcoT14I CCWWGG 3 cut(s) 258, 450, 599
EcoT38I GRGCYC 4 cut(s) 13, 257, 1030, 1089
EgeI GGCGCC 1 cut(s) 541
EheI GGCGCC 1 cut(s) 541
ErhI CCWWGG 3 cut(s) 258, 450, 599
FaeI CATG 6 cut(s) 31, 466, 562, 763, 847, 1015
FaqI GGGAC 4 cut(s) 468, 547, 617, 833
FatI CATG 6 cut(s) 27, 462, 558, 759, 843, 1011
FauI CCCGC 3 cut(s) 498, 748, 1030
FblI GTMKAC 1 cut(s) 767
Fnu4HI GCNGC 9 cut(s) 114, 117, 278, 281, 284, 287, 359, 471, 1105
FokI GGATG 4 cut(s) 362, 828, 853, 1067
FriOI GRGCYC 4 cut(s) 13, 257, 1030, 1089
Fsp4HI GCNGC 9 cut(s) 114, 117, 278, 281, 284, 287, 359, 471, 1105
FspBI CTAG 1 cut(s) 1211
GlaI GCGC 2 cut(s) 394, 541
GluI GCNGC 9 cut(s) 114, 117, 278, 281, 284, 287, 359, 471, 1105
GsaI CCCAGC 1 cut(s) 277
HaeII RGCGCY 1 cut(s) 543
HaeIII GGCC 5 cut(s) 90, 270, 664, 1107, 1255
HapII CCGG 3 cut(s) 87, 160, 1119
HhaI GCGC 2 cut(s) 395, 542
Hin1I GRCGYC 1 cut(s) 540
Hin1II CATG 6 cut(s) 31, 466, 562, 763, 847, 1015
Hin6I GCGC 2 cut(s) 393, 540
HinP1I GCGC 2 cut(s) 393, 540
HincII GTYRAC 1 cut(s) 768
HindII GTYRAC 1 cut(s) 768
HindIII AAGCTT 1 cut(s) 342
HinfI GANTC 5 cut(s) 217, 234, 397, 1150, 1238
HpaII CCGG 3 cut(s) 87, 160, 1119
HphI GGTGA 5 cut(s) 64, 139, 433, 454, 1157
Hpy166II GTNNAC 5 cut(s) 147, 301, 768, 931, 1228
Hpy188I TCNGA 8 cut(s) 170, 222, 349, 388, 411, 547, 969, 1243
Hpy188III TCNNGA 8 cut(s) 28, 229, 338, 401, 427, 797, 1090, 1235
Hpy8I GTNNAC 5 cut(s) 147, 301, 768, 931, 1228
Hpy99I CGWCG 3 cut(s) 181, 269, 772
HpyAV CCTTC 5 cut(s) 176, 505, 723, 964, 1241
HpyCH4III ACNGT 4 cut(s) 95, 892, 928, 1195
HpyCH4IV ACGT 1 cut(s) 770
HpyCH4V TGCA 4 cut(s) 328, 580, 900, 943
HpyF10VI GCNNNNNNNGC 6 cut(s) 261, 283, 286, 350, 364, 1034
HpyF3I CTNAG 4 cut(s) 354, 696, 1091, 1257
HpySE526I ACGT 1 cut(s) 770
Hsp92I GRCGYC 1 cut(s) 540
Hsp92II CATG 6 cut(s) 31, 466, 562, 763, 847, 1015
HspAI GCGC 2 cut(s) 393, 540
KasI GGCGCC 1 cut(s) 539
KflI GGGWCCC 1 cut(s) 455
KroI GCCGGC 1 cut(s) 86
KroNI GCCGGC 1 cut(s) 88
Kzo9I GATC 5 cut(s) 334, 423, 571, 676, 1057
LmnI GCTCC 7 cut(s) 260, 483, 619, 1025, 1033, 1084, 1092
Lsp1109I GCAGC 2 cut(s) 264, 273
LweI GCATC 1 cut(s) 340
MaeI CTAG 1 cut(s) 1211
MaeII ACGT 1 cut(s) 770
MaeIII GTNAC 2 cut(s) 442, 629
MalI GATC 5 cut(s) 336, 425, 573, 678, 1059
MbiI CCGCTC 1 cut(s) 358
MboI GATC 5 cut(s) 334, 423, 571, 676, 1057
MboII GAAGA 3 cut(s) 46, 52, 830
MhlI GDGCHC 5 cut(s) 13, 257, 624, 1030, 1089
MluCI AATT 8 cut(s) 163, 313, 610, 800, 856, 1138, 1171, 1246
Mly113I GGCGCC 1 cut(s) 540
MmeI TCCRAC 1 cut(s) 366
MroNI GCCGGC 1 cut(s) 86
MseI TTAA 2 cut(s) 35, 750
MslI CAYNNNNRTG 1 cut(s) 926
MspA1I CMGCKG 2 cut(s) 277, 707
MspI CCGG 3 cut(s) 87, 160, 1119
MspR9I CCNGG 2 cut(s) 681, 1119
MvaI CCWGG 1 cut(s) 681
MvnI CGCG 3 cut(s) 141, 395, 416
MwoI GCNNNNNNNGC 6 cut(s) 261, 283, 286, 350, 364, 1034
NaeI GCCGGC 1 cut(s) 88
NarI GGCGCC 1 cut(s) 540
NciI CCSGG 1 cut(s) 1119
NdeII GATC 5 cut(s) 334, 423, 571, 676, 1057
NgoMIV GCCGGC 1 cut(s) 86
NlaIII CATG 6 cut(s) 31, 466, 562, 763, 847, 1015
NlaIV GGNNCC 7 cut(s) 187, 256, 271, 456, 457, 541, 605
NmeAIII GCCGAG 1 cut(s) 516
NmuCI GTSAC 1 cut(s) 442
OliI CACNNNNGTG 1 cut(s) 926
PaeR7I CTCGAG 1 cut(s) 362
PagI TCATGA 1 cut(s) 27
PceI AGGCCT 2 cut(s) 664, 1255
PcsI WCGNNNNNNNCGW 3 cut(s) 138, 392, 432
PdiI GCCGGC 1 cut(s) 88
PfeI GAWTC 5 cut(s) 217, 234, 397, 1150, 1238
PflFI GACNNNGTC 1 cut(s) 1013
PkrI GCNGC 9 cut(s) 115, 118, 279, 282, 285, 288, 360, 472, 1106
PluTI GGCGCC 1 cut(s) 543
PpuMI RGGWCCY 2 cut(s) 455, 604
Psp124BI GAGCTC 3 cut(s) 13, 1030, 1089
Psp5II RGGWCCY 2 cut(s) 455, 604
Psp6I CCWGG 1 cut(s) 679
PspEI GGTNACC 1 cut(s) 442
PspFI CCCAGC 1 cut(s) 273
PspGI CCWGG 1 cut(s) 679
PspN4I GGNNCC 7 cut(s) 187, 256, 271, 456, 457, 541, 605
PspPI GGNCC 4 cut(s) 269, 455, 604, 1015
PspPPI RGGWCCY 2 cut(s) 455, 604
PspXI VCTCGAGB 1 cut(s) 362
PsyI GACNNNGTC 1 cut(s) 1013
PvuII CAGCTG 1 cut(s) 277
RsaI GTAC 4 cut(s) 689, 835, 848, 1042
RsaNI GTAC 4 cut(s) 688, 834, 847, 1041
RseI CAYNNNNRTG 1 cut(s) 926
SacI GAGCTC 3 cut(s) 13, 1030, 1089
SalI GTCGAC 1 cut(s) 766
SaqAI TTAA 2 cut(s) 35, 750
SatI GCNGC 9 cut(s) 114, 117, 278, 281, 284, 287, 359, 471, 1105
Sau3AI GATC 5 cut(s) 334, 423, 571, 676, 1057
Sau96I GGNCC 4 cut(s) 269, 455, 604, 1015
ScrFI CCNGG 2 cut(s) 681, 1119
SduI GDGCHC 5 cut(s) 13, 257, 624, 1030, 1089
SfaNI GCATC 1 cut(s) 340
SfcI CTRYAG 1 cut(s) 1194
SfoI GGCGCC 1 cut(s) 541
Sfr274I CTCGAG 1 cut(s) 362
SinI GGWCC 3 cut(s) 455, 604, 1015
SlaI CTCGAG 1 cut(s) 362
SmiMI CAYNNNNRTG 1 cut(s) 926
SmlI CTYRAG 1 cut(s) 362
SmoI CTYRAG 1 cut(s) 362
Sse9I AATT 8 cut(s) 163, 313, 610, 800, 856, 1138, 1171, 1246
SseBI AGGCCT 2 cut(s) 664, 1255
SspDI GGCGCC 1 cut(s) 539
SspMI CTAG 1 cut(s) 1211
SstI GAGCTC 3 cut(s) 13, 1030, 1089
StuI AGGCCT 2 cut(s) 664, 1255
StyD4I CCNGG 2 cut(s) 679, 1117
StyI CCWWGG 3 cut(s) 258, 450, 599
TaaI ACNGT 4 cut(s) 95, 892, 928, 1195
TaiI ACGT 1 cut(s) 773
TaqI TCGA 7 cut(s) 156, 333, 339, 363, 767, 798, 1077
TasI AATT 8 cut(s) 163, 313, 610, 800, 856, 1138, 1171, 1246
TatI WGTACW 1 cut(s) 846
TauI GCSGC 7 cut(s) 116, 119, 283, 286, 361, 473, 1107
TfiI GAWTC 5 cut(s) 217, 234, 397, 1150, 1238
Tru1I TTAA 2 cut(s) 35, 750
Tru9I TTAA 2 cut(s) 35, 750
TscAI CASTG 2 cut(s) 895, 1268
TseFI GTSAC 1 cut(s) 442
TseI GCWGC 2 cut(s) 277, 286
Tsp45I GTSAC 1 cut(s) 442
TspDTI ATGAA 1 cut(s) 17
TspGWI ACGGA 2 cut(s) 194, 1020
TspRI CASTG 2 cut(s) 895, 1268
Tth111I GACNNNGTC 1 cut(s) 1013
VpaK11BI GGWCC 3 cut(s) 455, 604, 1015
XapI RAATTY 5 cut(s) 163, 800, 1138, 1171, 1246
XhoI CTCGAG 1 cut(s) 362
XmiI GTMKAC 1 cut(s) 767
XspI CTAG 1 cut(s) 1211
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.