AT2G41660

MIZ1 MIZ1 (mizu-kussei 1)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
2
OG0014282
Physical Location & Seq
Forward (+)
17367492 .. 17369206
1715 bp
Loading structure...
UTR
Exon/CDS
Intron
AT2G41660.1

Sequence Viewer

Length: 894 bp
ATGGTGCCATACCAAGAACTCACTCTTCAGAGAAGCTTCAGCTACAACTCAAGAAAGATCAATCCAGTGACTTCTCCGGCGCGTAGCTCACACGTTCGTTCTCCTTCTTCCTCCGCCTTAATCCCAAGCATACCTGAGCACGAACTCTTCCTCGTTCCTTGCCGGAGATGCTCCTATGTCCCTCTCTCCTCCTCATCGTCGGCCTCTCACAATATCGGGAAATTTCACTTGAAGTTTTCTTTGCTCTTGCGTTCTTTCATCAACATCATTAACATCCCCGCTTGCAAAATGCTCTCTCTTCCCTCTCCGCCGTCTTCTTCTTCCAGCGTCTCTAACCAACTGATCTCACTCGTTACCGGTGGATCCTCTTCTCTCGGGAGAAGAGTCACCGGGACTCTGTACGGACACAAAAGAGGCCACGTCACGTTTTCGGTCCAGTACAACCAAAGATCCGACCCGGTCTTGCTCCTCGACCTGGCCATGTCAACGGCAACTCTCGTCAAAGAGATGTCGTCGGGGCTCGTCAGGATCGCGTTGGAGTGCGAGAAGCGTCATCGATCAGGTACGAAGCTTTTCCAAGAGCCCAAATGGACGATGTACTGCAACGGGAGGAAGTGCGGCTACGCTGTGTCACGTGGCGGCGCGTGTACTGATACAGATTGGCGCGTGTTGAACACGGTATCTAGGGTTACTGTTGGAGCCGGAGTTATTCCGACGCCGAAAACTATTGATGACGTGTCCGGTGTTGGTAGTGGAACGGAGCTTGGTGAGTTGTTGTATATGAGAGGCAAATTTGAACGAGTCGTTGGGAGCCGTGACTCGGAGGCGTTTTACATGATGAACCCGGACAAAAACGGAGGTCCAGAACTCAGTATTTTTCTTCTTAGAATATAA

Protein Analysis

297

Amino Acids

32.51

Weight (kDa)

9.75

Isoelectric Point (pI)

67.93

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF617 PF04759 129 - 296 6.7e-68 Protein of unknown function, DUF617
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014282)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G41660
fragaria_vesca FvH4_6g24150
malus_domestica MD12G1076100.v1.1 MD14G1074600.v1.1
prunus_persica Prupe.7G056000_v2.0.a1
pyrus_communis pycom14g06360
rosa_chinensis RchiOBHm_Chr3g0483021
rosa_laevigata RLG00000023306
rosa_multiflora Rmu_sc0001098.1_g000001 Rmu_sc0001098.1_g000002
rosa_roxburghii Rroxscaffold_6G00398730
rosa_rugosa Rorug03G0203100
rosa_samantha Rh3AG251700 Rh3BG288200 Rh3CG285700 Rh3DG281400
rosa_wichuraiana Rw3G022770

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 4
AccII CGCG 4 cut(s) 82, 533, 644, 666
AciI CCGC 5 cut(s) 114, 279, 308, 618, 639
AclWI GGATC 4 cut(s) 357, 370, 444, 536
AcoI YGGCCR 1 cut(s) 477
AcsI RAATTY 2 cut(s) 221, 791
AcuI CTGAAG 2 cut(s) 11, 22
AcvI CACGTG 1 cut(s) 635
AcyI GRCGYC 1 cut(s) 716
AfaI GTAC 5 cut(s) 401, 440, 565, 599, 649
AfiI CCNNNNNNNGG 2 cut(s) 475, 820
AflIII ACRYGT 2 cut(s) 91, 735
AgeI ACCGGT 1 cut(s) 356
AgsI TTSAA 3 cut(s) 232, 673, 797
AjiI CACGTC 2 cut(s) 421, 736
AjnI CCWGG 1 cut(s) 474
AjuI GAANNNNNNNTTGG 2 cut(s) 789, 821
AluBI AGCT 5 cut(s) 36, 42, 87, 571, 763
AluI AGCT 5 cut(s) 36, 42, 87, 571, 763
Alw21I GWGCWC 1 cut(s) 141
Alw26I GTCTC 1 cut(s) 334
AlwI GGATC 4 cut(s) 357, 370, 444, 536
Ama87I CYCGRG 1 cut(s) 374
AoxI GGCC 3 cut(s) 201, 415, 477
ApoI RAATTY 2 cut(s) 221, 791
AsiGI ACCGGT 1 cut(s) 356
Asp700I GAANNNNTTC 1 cut(s) 572
AspLEI GCGC 3 cut(s) 82, 644, 666
AspS9I GGNCC 2 cut(s) 433, 860
AsuC2I CCSGG 3 cut(s) 391, 458, 845
AsuHPI GGTGA 2 cut(s) 379, 779
AvaI CYCGRG 1 cut(s) 374
AvaII GGWCC 2 cut(s) 433, 860
BalI TGGCCA 1 cut(s) 479
BamHI GGATCC 1 cut(s) 362
BanI GGYRCC 1 cut(s) 4
BanII GRGCYC 2 cut(s) 522, 585
BarI GAAGNNNNNNTAC 2 cut(s) 605, 637
BbrPI CACGTG 1 cut(s) 635
BbsI GAAGAC 1 cut(s) 306
Bbv12I GWGCWC 1 cut(s) 141
BceAI ACGGC 3 cut(s) 295, 504, 798
BciT130I CCWGG 1 cut(s) 476
BcnI CCSGG 3 cut(s) 391, 458, 845
BcoDI GTCTC 1 cut(s) 334
BfaI CTAG 1 cut(s) 684
BisI GCNGC 2 cut(s) 619, 640
BlsI GCNGC 2 cut(s) 620, 641
Bme1390I CCNGG 4 cut(s) 391, 458, 476, 845
Bme18I GGWCC 2 cut(s) 433, 860
BmeT110I CYCGRG 1 cut(s) 374
BmgBI CACGTC 2 cut(s) 421, 736
BmgT120I GGNCC 2 cut(s) 433, 860
BmiI GGNNCC 4 cut(s) 6, 364, 700, 812
BmrFI CCNGG 4 cut(s) 391, 458, 476, 845
BmsI GCATC 1 cut(s) 158
BpiI GAAGAC 1 cut(s) 306
Bpu10I CCTNAGC 1 cut(s) 135
BpuEI CTTGAG 1 cut(s) 34
BpuMI CCSGG 3 cut(s) 391, 458, 845
Bsa29I ATCGAT 1 cut(s) 556
BsaAI YACGTR 1 cut(s) 635
BsaHI GRCGYC 1 cut(s) 716
BsaWI WCCGGW 2 cut(s) 356, 740
BsaXI ACNNNNNCTCC 2 cut(s) 690, 720
Bsc4I CCNNNNNNNGG 2 cut(s) 475, 820
Bse118I RCCGGY 1 cut(s) 356
Bse1I ACTGG 2 cut(s) 65, 436
BseBI CCWGG 1 cut(s) 476
BseCI ATCGAT 1 cut(s) 556
BseGI GGATG 1 cut(s) 273
BseLI CCNNNNNNNGG 2 cut(s) 475, 820
BseMII CTCAG 2 cut(s) 126, 883
BseNI ACTGG 2 cut(s) 65, 436
BseRI GAGGAG 3 cut(s) 178, 181, 458
Bsh1236I CGCG 4 cut(s) 82, 533, 644, 666
BshFI GGCC 3 cut(s) 203, 417, 479
BshNI GGYRCC 1 cut(s) 4
BshTI ACCGGT 1 cut(s) 356
BshVI ATCGAT 1 cut(s) 556
BsiHKAI GWGCWC 1 cut(s) 141
BsiHKCI CYCGRG 1 cut(s) 374
BsiSI CCGG 8 cut(s) 77, 163, 357, 390, 458, 702, 741, 845
BslFI GGGAC 2 cut(s) 164, 406
BslI CCNNNNNNNGG 2 cut(s) 475, 820
BsmAI GTCTC 1 cut(s) 334
BsmBI CGTCTC 1 cut(s) 334
BsmFI GGGAC 2 cut(s) 164, 406
BsnI GGCC 3 cut(s) 203, 417, 479
BsoBI CYCGRG 1 cut(s) 374
Bsp1286I GDGCHC 3 cut(s) 141, 522, 585
Bsp143I GATC 6 cut(s) 57, 342, 362, 449, 528, 557
BspACI CCGC 5 cut(s) 114, 279, 308, 618, 639
BspANI GGCC 3 cut(s) 203, 417, 479
BspCNI CTCAG 2 cut(s) 127, 882
BspDI ATCGAT 1 cut(s) 556
BspFNI CGCG 4 cut(s) 82, 533, 644, 666
BspLI GGNNCC 4 cut(s) 6, 364, 700, 812
BspPI GGATC 4 cut(s) 357, 370, 444, 536
BspT107I GGYRCC 1 cut(s) 4
BsrFI RCCGGY 1 cut(s) 356
BsrI ACTGG 2 cut(s) 65, 436
BssAI RCCGGY 1 cut(s) 356
BssMI GATC 6 cut(s) 57, 342, 362, 449, 528, 557
BssNI GRCGYC 1 cut(s) 716
Bst2UI CCWGG 1 cut(s) 476
Bst4CI ACNGT 2 cut(s) 679, 694
Bst6I CTCTTC 5 cut(s) 30, 152, 303, 373, 376
BstACI GRCGYC 1 cut(s) 716
BstBAI YACGTR 1 cut(s) 635
BstC8I GCNNGC 1 cut(s) 283
BstDEI CTNAG 3 cut(s) 135, 869, 884
BstF5I GGATG 1 cut(s) 273
BstFNI CGCG 4 cut(s) 82, 533, 644, 666
BstHHI GCGC 3 cut(s) 82, 644, 666
BstKTI GATC 6 cut(s) 60, 345, 365, 452, 531, 560
BstMAI GTCTC 1 cut(s) 334
BstMBI GATC 6 cut(s) 57, 342, 362, 449, 528, 557
BstMWI GCNNNNNNNGC 1 cut(s) 168
BstNI CCWGG 1 cut(s) 476
BstSCI CCNGG 4 cut(s) 389, 456, 474, 843
BstUI CGCG 4 cut(s) 82, 533, 644, 666
BstV2I GAAGAC 1 cut(s) 306
BstX2I RGATCY 2 cut(s) 362, 449
BstYI RGATCY 2 cut(s) 362, 449
Bsu15I ATCGAT 1 cut(s) 556
BsuRI GGCC 3 cut(s) 203, 417, 479
BsuTUI ATCGAT 1 cut(s) 556
BtrI CACGTC 2 cut(s) 421, 736
BtsCI GGATG 1 cut(s) 273
BtsIMutI CAGTG 1 cut(s) 72
Cac8I GCNNGC 1 cut(s) 283
CfoI GCGC 3 cut(s) 82, 644, 666
Cfr10I RCCGGY 1 cut(s) 356
Cfr13I GGNCC 2 cut(s) 433, 860
ClaI ATCGAT 1 cut(s) 556
CseI GACGC 3 cut(s) 316, 539, 724
Csp6I GTAC 5 cut(s) 400, 439, 564, 598, 648
CspAI ACCGGT 1 cut(s) 356
CviAII CATG 2 cut(s) 481, 835
CviQI GTAC 5 cut(s) 400, 439, 564, 598, 648
DdeI CTNAG 3 cut(s) 135, 869, 884
DpnI GATC 6 cut(s) 59, 344, 364, 451, 530, 559
DpnII GATC 6 cut(s) 57, 342, 362, 449, 528, 557
EaeI YGGCCR 1 cut(s) 477
Eam1104I CTCTTC 5 cut(s) 30, 152, 303, 373, 376
EarI CTCTTC 5 cut(s) 30, 152, 303, 373, 376
EciI GGCGGA 2 cut(s) 103, 297
Eco24I GRGCYC 2 cut(s) 522, 585
Eco47I GGWCC 2 cut(s) 433, 860
Eco57I CTGAAG 2 cut(s) 11, 22
Eco72I CACGTG 1 cut(s) 635
Eco88I CYCGRG 1 cut(s) 374
EcoRII CCWGG 1 cut(s) 474
EcoT38I GRGCYC 2 cut(s) 522, 585
Esp3I CGTCTC 1 cut(s) 334
FaeI CATG 2 cut(s) 484, 838
FaiI YATR 8 cut(s) 10, 131, 177, 482, 780, 782, 836, 892
FaqI GGGAC 2 cut(s) 164, 406
FatI CATG 2 cut(s) 480, 834
FauI CCCGC 1 cut(s) 286
Fnu4HI GCNGC 2 cut(s) 619, 640
FokI GGATG 1 cut(s) 260
FriOI GRGCYC 2 cut(s) 522, 585
Fsp4HI GCNGC 2 cut(s) 619, 640
FspBI CTAG 1 cut(s) 684
GlaI GCGC 3 cut(s) 81, 643, 665
GluI GCNGC 2 cut(s) 619, 640
HaeIII GGCC 3 cut(s) 203, 417, 479
HapII CCGG 8 cut(s) 77, 163, 357, 390, 458, 702, 741, 845
HgaI GACGC 3 cut(s) 316, 539, 724
HhaI GCGC 3 cut(s) 82, 644, 666
Hin1I GRCGYC 1 cut(s) 716
Hin1II CATG 2 cut(s) 484, 838
Hin6I GCGC 3 cut(s) 80, 642, 664
HinP1I GCGC 3 cut(s) 80, 642, 664
HincII GTYRAC 1 cut(s) 486
HindII GTYRAC 1 cut(s) 486
HindIII AAGCTT 2 cut(s) 34, 569
HinfI GANTC 4 cut(s) 384, 394, 801, 818
HpaII CCGG 8 cut(s) 77, 163, 357, 390, 458, 702, 741, 845
HphI GGTGA 2 cut(s) 379, 779
Hpy166II GTNNAC 2 cut(s) 486, 648
Hpy188I TCNGA 4 cut(s) 30, 454, 714, 823
Hpy188III TCNNGA 5 cut(s) 51, 217, 376, 526, 863
Hpy8I GTNNAC 2 cut(s) 486, 648
Hpy99I CGWCG 3 cut(s) 202, 517, 718
HpyAV CCTTC 1 cut(s) 114
HpyCH4III ACNGT 2 cut(s) 679, 694
HpyCH4IV ACGT 5 cut(s) 93, 420, 425, 634, 735
HpyCH4V TGCA 2 cut(s) 285, 603
HpyF10VI GCNNNNNNNGC 1 cut(s) 168
HpyF3I CTNAG 3 cut(s) 135, 869, 884
HpySE526I ACGT 5 cut(s) 93, 420, 425, 634, 735
Hsp92I GRCGYC 1 cut(s) 716
Hsp92II CATG 2 cut(s) 484, 838
HspAI GCGC 3 cut(s) 80, 642, 664
Kzo9I GATC 6 cut(s) 57, 342, 362, 449, 528, 557
LmnI GCTCC 5 cut(s) 176, 471, 698, 760, 810
LweI GCATC 1 cut(s) 158
MaeI CTAG 1 cut(s) 684
MaeII ACGT 5 cut(s) 93, 420, 425, 634, 735
MaeIII GTNAC 7 cut(s) 67, 352, 385, 421, 630, 688, 815
MalI GATC 6 cut(s) 59, 344, 364, 451, 530, 559
MboI GATC 6 cut(s) 57, 342, 362, 449, 528, 557
MflI RGATCY 2 cut(s) 362, 449
MhlI GDGCHC 3 cut(s) 141, 522, 585
MlsI TGGCCA 1 cut(s) 479
MluCI AATT 2 cut(s) 221, 791
MluNI TGGCCA 1 cut(s) 479
MlyI GAGTC 4 cut(s) 388, 393, 810, 812
MmeI TCCRAC 4 cut(s) 477, 516, 676, 737
Mox20I TGGCCA 1 cut(s) 479
MroXI GAANNNNTTC 1 cut(s) 572
MscI TGGCCA 1 cut(s) 479
MseI TTAA 2 cut(s) 119, 270
Msp20I TGGCCA 1 cut(s) 479
MspI CCGG 8 cut(s) 77, 163, 357, 390, 458, 702, 741, 845
MspR9I CCNGG 4 cut(s) 391, 458, 476, 845
MvaI CCWGG 1 cut(s) 476
MvnI CGCG 4 cut(s) 82, 533, 644, 666
MwoI GCNNNNNNNGC 1 cut(s) 168
NciI CCSGG 3 cut(s) 391, 458, 845
NdeII GATC 6 cut(s) 57, 342, 362, 449, 528, 557
NlaIII CATG 2 cut(s) 484, 838
NlaIV GGNNCC 4 cut(s) 6, 364, 700, 812
NmuCI GTSAC 5 cut(s) 67, 385, 421, 630, 815
PdmI GAANNNNTTC 1 cut(s) 572
PflFI GACNNNGTC 1 cut(s) 458
PinAI ACCGGT 1 cut(s) 356
PkrI GCNGC 2 cut(s) 620, 641
PleI GAGTC 4 cut(s) 388, 392, 809, 812
PmaCI CACGTG 1 cut(s) 635
PmlI CACGTG 1 cut(s) 635
PpsI GAGTC 4 cut(s) 388, 392, 809, 812
Ppu21I YACGTR 1 cut(s) 635
Psp6I CCWGG 1 cut(s) 474
PspCI CACGTG 1 cut(s) 635
PspGI CCWGG 1 cut(s) 474
PspN4I GGNNCC 4 cut(s) 6, 364, 700, 812
PspPI GGNCC 2 cut(s) 433, 860
PsuI RGATCY 2 cut(s) 362, 449
PsyI GACNNNGTC 1 cut(s) 458
RsaI GTAC 5 cut(s) 401, 440, 565, 599, 649
RsaNI GTAC 5 cut(s) 400, 439, 564, 598, 648
SaqAI TTAA 2 cut(s) 119, 270
SatI GCNGC 2 cut(s) 619, 640
Sau3AI GATC 6 cut(s) 57, 342, 362, 449, 528, 557
Sau96I GGNCC 2 cut(s) 433, 860
SchI GAGTC 4 cut(s) 388, 393, 810, 812
ScrFI CCNGG 4 cut(s) 391, 458, 476, 845
SduI GDGCHC 3 cut(s) 141, 522, 585
SfaNI GCATC 1 cut(s) 158
SinI GGWCC 2 cut(s) 433, 860
SmlI CTYRAG 1 cut(s) 49
SmoI CTYRAG 1 cut(s) 49
Sse9I AATT 2 cut(s) 221, 791
SsiI CCGC 5 cut(s) 114, 279, 308, 618, 639
SspMI CTAG 1 cut(s) 684
StyD4I CCNGG 4 cut(s) 389, 456, 474, 843
TaaI ACNGT 2 cut(s) 679, 694
TaiI ACGT 5 cut(s) 96, 423, 428, 637, 738
TaqI TCGA 2 cut(s) 471, 556
TaqII GACCGA 1 cut(s) 421
TasI AATT 2 cut(s) 221, 791
TatI WGTACW 3 cut(s) 438, 597, 647
TauI GCSGC 2 cut(s) 621, 642
Tru1I TTAA 2 cut(s) 119, 270
Tru9I TTAA 2 cut(s) 119, 270
TscAI CASTG 1 cut(s) 72
TseFI GTSAC 5 cut(s) 67, 385, 421, 630, 815
Tsp45I GTSAC 5 cut(s) 67, 385, 421, 630, 815
TspDTI ATGAA 2 cut(s) 247, 854
TspGWI ACGGA 3 cut(s) 417, 773, 870
TspRI CASTG 1 cut(s) 72
Tth111I GACNNNGTC 1 cut(s) 458
VpaK11BI GGWCC 2 cut(s) 433, 860
XapI RAATTY 2 cut(s) 221, 791
XmnI GAANNNNTTC 1 cut(s) 572
XspI CTAG 1 cut(s) 684
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.