MD12G1076100.v1.1

Protein of unknown function, DUF617

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0014282
Physical Location & Seq
Reverse (-)
9213087 .. 9213926
840 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1076100.v1.1.491

Sequence Viewer

Length: 840 bp
ATGAAACACCAAGACCTCAACCTTCAAAGAAGCACCAGTACTACTTCCATATCAAGAAGTACTTCGAGAAAGATCATCCCATCCAACCACTTCAGATCTTCATCATCAACTTTCCCACTTAACCATCTTCAAAACGACGACGTCGCCAACAACCTTTTGGTTCCGAAACACCAATCGCCAGTCTCCTTCCACCACCTCTCCAAAACCCAAACAAAACTCACCTCACTCCTCCGCTCTTTCCTCAGCTTCCTCTCCTTCCCCACCATTATCCCAACATGCAAATGGCTCGCCCTCACCTCCAACCTCTCCGTCACTCCCTCCCTTGGCCGAAAAGTCACCGGCACACTCTTCGGAAACCGCCGTGGCCACGTCAGCTTCGCCGTCCAGCTGGACCCACAGTCGGAGCCTGTTTTTCTTCTCGAGCTTGCCATGTCAACGTCTTCACTCGTTAAAGAGATGTCTTCCGGGCTCGTGCGCATTGCTCTCGAGTCTGAAAAGCAACAGGGTTCTGGCCGTACAGGTATGCATGGTCGGAAGCTGTTTCAGGAGCCTTCATGGACTATGTATTGTAACGGGAGGAAGTGTGGGTATGCGGCGTCTCGCACATGCGGCGAGTCAGACTGGCACGTGCTGAGGACTATTCGAAGCGTGTCGGTCGGAGCCGGCGTGATTCCAGTGGTGGACGACGGAAAAAAGGGTGGCGCGTCTGAAGGTGAGTTGCTTTATATGAGAGCTAGGTTTGAACGAGTCGTGGGAAGCCGTGACTCGGAGGCCTTTTACATGTTGAACCCTGAGGGTAATGGAGGTCCTGAACTCAGTATCTTTTTACTCAGAATATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF617

Protein Analysis

280

Amino Acids

30.79

Weight (kDa)

9.88

Isoelectric Point (pI)

56.08

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF617 PF04759 112 - 278 4.6e-70 Protein of unknown function, DUF617
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014282)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G41660
fragaria_vesca FvH4_6g24150
malus_domestica MD12G1076100.v1.1 MD14G1074600.v1.1
prunus_persica Prupe.7G056000_v2.0.a1
pyrus_communis pycom14g06360
rosa_chinensis RchiOBHm_Chr3g0483021
rosa_laevigata RLG00000023306
rosa_multiflora Rmu_sc0001098.1_g000001 Rmu_sc0001098.1_g000002
rosa_roxburghii Rroxscaffold_6G00398730
rosa_rugosa Rorug03G0203100
rosa_samantha Rh3AG251700 Rh3BG288200 Rh3CG285700 Rh3DG281400
rosa_wichuraiana Rw3G022770

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 144
Acc16I TGCGCA 1 cut(s) 476
AccBSI CCGCTC 1 cut(s) 234
AccII CGCG 1 cut(s) 704
AciI CCGC 4 cut(s) 232, 358, 593, 609
AcoI YGGCCR 3 cut(s) 325, 364, 511
AcuI CTGAAG 2 cut(s) 76, 729
AcvI CACGTG 1 cut(s) 628
AcyI GRCGYC 2 cut(s) 141, 596
AfaI GTAC 3 cut(s) 40, 61, 517
AfiI CCNNNNNNNGG 3 cut(s) 323, 400, 766
AflIII ACRYGT 1 cut(s) 780
AgsI TTSAA 4 cut(s) 26, 131, 743, 787
AhdI GACNNNNNGTC 1 cut(s) 397
AjiI CACGTC 1 cut(s) 370
AluBI AGCT 6 cut(s) 246, 375, 388, 424, 538, 734
AluI AGCT 6 cut(s) 246, 375, 388, 424, 538, 734
Alw26I GTCTC 2 cut(s) 187, 603
Ama87I CYCGRG 2 cut(s) 419, 485
AoxI GGCC 4 cut(s) 325, 364, 511, 771
Asp700I GAANNNNTTC 1 cut(s) 61
AspLEI GCGC 2 cut(s) 477, 704
AspS9I GGNCC 2 cut(s) 391, 806
AsuC2I CCSGG 1 cut(s) 466
AsuHPI GGTGA 4 cut(s) 211, 286, 328, 725
AsuII TTCGAA 1 cut(s) 643
AvaI CYCGRG 2 cut(s) 419, 485
AvaII GGWCC 2 cut(s) 391, 806
AxyI CCTNAGG 1 cut(s) 792
BalI TGGCCA 1 cut(s) 366
BanII GRGCYC 1 cut(s) 471
BarI GAAGNNNNNNTAC 2 cut(s) 22, 54
BauI CACGAG 1 cut(s) 470
BbrPI CACGTG 1 cut(s) 628
BbsI GAAGAC 2 cut(s) 432, 453
BbvCI CCTCAGC 2 cut(s) 242, 632
BccI CCATC 2 cut(s) 88, 132
BceAI ACGGC 4 cut(s) 345, 365, 498, 744
BcgI CGANNNNNNTGC 6 cut(s) 268, 302, 331, 365, 466, 500
BcnI CCSGG 1 cut(s) 466
BcoDI GTCTC 2 cut(s) 187, 603
BfaI CTAG 1 cut(s) 735
BglII AGATCT 1 cut(s) 95
BisI GCNGC 2 cut(s) 594, 610
BlsI GCNGC 2 cut(s) 595, 611
BmcAI AGTACT 2 cut(s) 40, 61
Bme1390I CCNGG 1 cut(s) 466
Bme18I GGWCC 2 cut(s) 391, 806
BmeRI GACNNNNNGTC 1 cut(s) 397
BmeT110I CYCGRG 2 cut(s) 419, 485
BmgBI CACGTC 1 cut(s) 370
BmgT120I GGNCC 2 cut(s) 391, 806
BmiI GGNNCC 5 cut(s) 162, 393, 405, 549, 661
BmrFI CCNGG 1 cut(s) 466
BpiI GAAGAC 2 cut(s) 432, 453
Bpu10I CCTNAGC 2 cut(s) 242, 632
Bpu14I TTCGAA 1 cut(s) 643
BpuMI CCSGG 1 cut(s) 466
BsaAI YACGTR 1 cut(s) 628
BsaBI GATNNNNATC 1 cut(s) 100
BsaHI GRCGYC 2 cut(s) 141, 596
BsaJI CCNNGG 2 cut(s) 322, 361
BsaXI ACNNNNNCTCC 6 cut(s) 182, 212, 568, 598, 651, 681
Bsc4I CCNNNNNNNGG 3 cut(s) 323, 400, 766
Bse118I RCCGGY 2 cut(s) 338, 662
Bse1I ACTGG 4 cut(s) 36, 179, 626, 674
Bse21I CCTNAGG 1 cut(s) 792
Bse3DI GCAATG 1 cut(s) 477
Bse8I GATNNNNATC 1 cut(s) 100
BseDI CCNNGG 2 cut(s) 322, 361
BseGI GGATG 2 cut(s) 75, 80
BseJI GATNNNNATC 1 cut(s) 100
BseLI CCNNNNNNNGG 3 cut(s) 323, 400, 766
BseMI GCAATG 1 cut(s) 477
BseMII CTCAG 4 cut(s) 256, 623, 783, 829
BseNI ACTGG 4 cut(s) 36, 179, 626, 674
BseRI GAGGAG 1 cut(s) 218
Bsh1236I CGCG 1 cut(s) 704
Bsh1285I CGRYCG 1 cut(s) 657
BshFI GGCC 4 cut(s) 327, 366, 513, 773
BsiEI CGRYCG 1 cut(s) 657
BsiHKCI CYCGRG 2 cut(s) 419, 485
BsiSI CCGG 3 cut(s) 339, 465, 663
BslI CCNNNNNNNGG 3 cut(s) 323, 400, 766
BsmAI GTCTC 2 cut(s) 187, 603
BsmBI CGTCTC 1 cut(s) 603
BsnI GGCC 4 cut(s) 327, 366, 513, 773
BsoBI CYCGRG 2 cut(s) 419, 485
Bsp119I TTCGAA 1 cut(s) 643
Bsp1286I GDGCHC 1 cut(s) 471
Bsp143I GATC 2 cut(s) 72, 95
BspACI CCGC 4 cut(s) 232, 358, 593, 609
BspANI GGCC 4 cut(s) 327, 366, 513, 773
BspCNI CTCAG 4 cut(s) 255, 624, 784, 828
BspFNI CGCG 1 cut(s) 704
BspLI GGNNCC 5 cut(s) 162, 393, 405, 549, 661
BspT104I TTCGAA 1 cut(s) 643
BsrBI CCGCTC 1 cut(s) 234
BsrDI GCAATG 1 cut(s) 477
BsrFI RCCGGY 2 cut(s) 338, 662
BsrI ACTGG 4 cut(s) 36, 179, 626, 674
BssAI RCCGGY 2 cut(s) 338, 662
BssECI CCNNGG 2 cut(s) 322, 361
BssMI GATC 2 cut(s) 72, 95
BssNI GRCGYC 2 cut(s) 141, 596
BssSI CACGAG 1 cut(s) 470
BssT1I CCWWGG 1 cut(s) 322
Bst2BI CACGAG 1 cut(s) 470
Bst4CI ACNGT 1 cut(s) 399
Bst6I CTCTTC 1 cut(s) 353
BstACI GRCGYC 2 cut(s) 141, 596
BstBAI YACGTR 1 cut(s) 628
BstBI TTCGAA 1 cut(s) 643
BstC8I GCNNGC 3 cut(s) 288, 426, 664
BstDEI CTNAG 5 cut(s) 242, 632, 792, 815, 830
BstDSI CCRYGG 1 cut(s) 361
BstF5I GGATG 2 cut(s) 75, 80
BstFNI CGCG 1 cut(s) 704
BstHHI GCGC 2 cut(s) 477, 704
BstKTI GATC 2 cut(s) 75, 98
BstMAI GTCTC 2 cut(s) 187, 603
BstMBI GATC 2 cut(s) 72, 95
BstMCI CGRYCG 1 cut(s) 657
BstMWI GCNNNNNNNGC 2 cut(s) 372, 609
BstNSI RCATGY 3 cut(s) 279, 609, 784
BstSCI CCNGG 1 cut(s) 464
BstUI CGCG 1 cut(s) 704
BstV2I GAAGAC 2 cut(s) 432, 453
BstX2I RGATCY 1 cut(s) 95
BstYI RGATCY 1 cut(s) 95
Bsu36I CCTNAGG 1 cut(s) 792
BsuRI GGCC 4 cut(s) 327, 366, 513, 773
BtgI CCRYGG 1 cut(s) 361
BtrI CACGTC 1 cut(s) 370
BtsCI GGATG 2 cut(s) 75, 80
BtsIMutI CAGTG 1 cut(s) 681
Cac8I GCNNGC 3 cut(s) 288, 426, 664
CfoI GCGC 2 cut(s) 477, 704
Cfr10I RCCGGY 2 cut(s) 338, 662
Cfr13I GGNCC 2 cut(s) 391, 806
CseI GACGC 2 cut(s) 585, 693
Csp6I GTAC 3 cut(s) 39, 60, 516
CviAII CATG 6 cut(s) 276, 430, 527, 555, 606, 781
CviQI GTAC 3 cut(s) 39, 60, 516
DdeI CTNAG 5 cut(s) 242, 632, 792, 815, 830
DpnI GATC 2 cut(s) 74, 97
DpnII GATC 2 cut(s) 72, 95
DriI GACNNNNNGTC 1 cut(s) 397
EaeI YGGCCR 3 cut(s) 325, 364, 511
Eam1104I CTCTTC 1 cut(s) 353
Eam1105I GACNNNNNGTC 1 cut(s) 397
EarI CTCTTC 1 cut(s) 353
Eco130I CCWWGG 1 cut(s) 322
Eco147I AGGCCT 1 cut(s) 773
Eco24I GRGCYC 1 cut(s) 471
Eco47I GGWCC 2 cut(s) 391, 806
Eco57I CTGAAG 2 cut(s) 76, 729
Eco72I CACGTG 1 cut(s) 628
Eco81I CCTNAGG 1 cut(s) 792
Eco88I CYCGRG 2 cut(s) 419, 485
EcoO109I RGGNCCY 1 cut(s) 806
EcoT14I CCWWGG 1 cut(s) 322
EcoT22I ATGCAT 1 cut(s) 528
EcoT38I GRGCYC 1 cut(s) 471
ErhI CCWWGG 1 cut(s) 322
Esp3I CGTCTC 1 cut(s) 603
FaeI CATG 6 cut(s) 279, 433, 530, 558, 609, 784
FalI AAGNNNNNCTT 2 cut(s) 46, 78
FatI CATG 6 cut(s) 275, 429, 526, 554, 605, 780
Fnu4HI GCNGC 2 cut(s) 594, 610
FokI GGATG 2 cut(s) 62, 67
FriOI GRGCYC 1 cut(s) 471
Fsp4HI GCNGC 2 cut(s) 594, 610
FspAI RTGCGCAY 1 cut(s) 476
FspBI CTAG 1 cut(s) 735
FspI TGCGCA 1 cut(s) 476
GlaI GCGC 2 cut(s) 476, 703
GluI GCNGC 2 cut(s) 594, 610
HaeIII GGCC 4 cut(s) 327, 366, 513, 773
HapII CCGG 3 cut(s) 339, 465, 663
HgaI GACGC 2 cut(s) 585, 693
HhaI GCGC 2 cut(s) 477, 704
Hin1I GRCGYC 2 cut(s) 141, 596
Hin1II CATG 6 cut(s) 279, 433, 530, 558, 609, 784
Hin6I GCGC 2 cut(s) 475, 702
HinP1I GCGC 2 cut(s) 475, 702
HincII GTYRAC 1 cut(s) 435
HindII GTYRAC 1 cut(s) 435
HinfI GANTC 5 cut(s) 488, 614, 670, 747, 764
HpaII CCGG 3 cut(s) 339, 465, 663
HphI GGTGA 4 cut(s) 211, 286, 328, 725
Hpy166II GTNNAC 2 cut(s) 435, 682
Hpy188III TCNNGA 6 cut(s) 54, 66, 419, 485, 545, 809
Hpy8I GTNNAC 2 cut(s) 435, 682
Hpy99I CGWCG 4 cut(s) 140, 143, 146, 689
HpyAV CCTTC 5 cut(s) 32, 196, 265, 561, 704
HpyCH4III ACNGT 1 cut(s) 399
HpyCH4IV ACGT 4 cut(s) 141, 369, 437, 627
HpyCH4V TGCA 2 cut(s) 279, 526
HpyF10VI GCNNNNNNNGC 2 cut(s) 372, 609
HpyF3I CTNAG 5 cut(s) 242, 632, 792, 815, 830
HpySE526I ACGT 4 cut(s) 141, 369, 437, 627
Hsp92I GRCGYC 2 cut(s) 141, 596
Hsp92II CATG 6 cut(s) 279, 433, 530, 558, 609, 784
HspAI GCGC 2 cut(s) 475, 702
KroI GCCGGC 1 cut(s) 662
KroNI GCCGGC 1 cut(s) 664
Kzo9I GATC 2 cut(s) 72, 95
LmnI GCTCC 3 cut(s) 403, 547, 659
MaeI CTAG 1 cut(s) 735
MaeII ACGT 4 cut(s) 141, 369, 437, 627
MaeIII GTNAC 4 cut(s) 310, 334, 569, 761
MalI GATC 2 cut(s) 74, 97
MbiI CCGCTC 1 cut(s) 234
MboI GATC 2 cut(s) 72, 95
MboII GAAGA 6 cut(s) 90, 119, 340, 407, 432, 453
MflI RGATCY 1 cut(s) 95
MhlI GDGCHC 1 cut(s) 471
MlsI TGGCCA 1 cut(s) 366
MluNI TGGCCA 1 cut(s) 366
MlyI GAGTC 4 cut(s) 497, 623, 756, 758
MmeI TCCRAC 5 cut(s) 108, 324, 381, 512, 637
Mox20I TGGCCA 1 cut(s) 366
Mph1103I ATGCAT 1 cut(s) 528
MroNI GCCGGC 1 cut(s) 662
MroXI GAANNNNTTC 1 cut(s) 61
MscI TGGCCA 1 cut(s) 366
MseI TTAA 2 cut(s) 120, 450
MslI CAYNNNNRTG 1 cut(s) 280
Msp20I TGGCCA 1 cut(s) 366
MspA1I CMGCKG 1 cut(s) 388
MspI CCGG 3 cut(s) 339, 465, 663
MspR9I CCNGG 1 cut(s) 466
MvnI CGCG 1 cut(s) 704
MwoI GCNNNNNNNGC 2 cut(s) 372, 609
NaeI GCCGGC 1 cut(s) 664
NciI CCSGG 1 cut(s) 466
NdeII GATC 2 cut(s) 72, 95
NgoMIV GCCGGC 1 cut(s) 662
NlaIII CATG 6 cut(s) 279, 433, 530, 558, 609, 784
NlaIV GGNNCC 5 cut(s) 162, 393, 405, 549, 661
NmuCI GTSAC 3 cut(s) 310, 334, 761
NsbI TGCGCA 1 cut(s) 476
NsiI ATGCAT 1 cut(s) 528
NspI RCATGY 3 cut(s) 279, 609, 784
NspV TTCGAA 1 cut(s) 643
PaeR7I CTCGAG 2 cut(s) 419, 485
PceI AGGCCT 1 cut(s) 773
PciI ACATGT 1 cut(s) 780
PcsI WCGNNNNNNNCGW 1 cut(s) 663
PdiI GCCGGC 1 cut(s) 664
PdmI GAANNNNTTC 1 cut(s) 61
PfeI GAWTC 1 cut(s) 670
PflFI GACNNNGTC 1 cut(s) 140
PkrI GCNGC 2 cut(s) 595, 611
PleI GAGTC 4 cut(s) 496, 622, 755, 758
PmaCI CACGTG 1 cut(s) 628
PmlI CACGTG 1 cut(s) 628
PpsI GAGTC 4 cut(s) 496, 622, 755, 758
Ppu21I YACGTR 1 cut(s) 628
PpuMI RGGWCCY 1 cut(s) 806
PscI ACATGT 1 cut(s) 780
Psp5II RGGWCCY 1 cut(s) 806
PspCI CACGTG 1 cut(s) 628
PspN4I GGNNCC 5 cut(s) 162, 393, 405, 549, 661
PspPI GGNCC 2 cut(s) 391, 806
PspPPI RGGWCCY 1 cut(s) 806
PsuI RGATCY 1 cut(s) 95
PsyI GACNNNGTC 1 cut(s) 140
PvuII CAGCTG 1 cut(s) 388
RsaI GTAC 3 cut(s) 40, 61, 517
RsaNI GTAC 3 cut(s) 39, 60, 516
RseI CAYNNNNRTG 1 cut(s) 280
SaqAI TTAA 2 cut(s) 120, 450
SatI GCNGC 2 cut(s) 594, 610
Sau3AI GATC 2 cut(s) 72, 95
Sau96I GGNCC 2 cut(s) 391, 806
ScaI AGTACT 2 cut(s) 40, 61
SchI GAGTC 4 cut(s) 497, 623, 756, 758
ScrFI CCNGG 1 cut(s) 466
SduI GDGCHC 1 cut(s) 471
Sfr274I CTCGAG 2 cut(s) 419, 485
SfuI TTCGAA 1 cut(s) 643
SinI GGWCC 2 cut(s) 391, 806
SlaI CTCGAG 2 cut(s) 419, 485
SmiMI CAYNNNNRTG 1 cut(s) 280
SmlI CTYRAG 2 cut(s) 419, 485
SmoI CTYRAG 2 cut(s) 419, 485
SseBI AGGCCT 1 cut(s) 773
SsiI CCGC 4 cut(s) 232, 358, 593, 609
SspMI CTAG 1 cut(s) 735
StuI AGGCCT 1 cut(s) 773
StyD4I CCNGG 1 cut(s) 464
StyI CCWWGG 1 cut(s) 322
TaaI ACNGT 1 cut(s) 399
TaiI ACGT 4 cut(s) 144, 372, 440, 630
TaqI TCGA 4 cut(s) 65, 420, 486, 643
TaqII GACCGA 1 cut(s) 643
TatI WGTACW 2 cut(s) 38, 59
TauI GCSGC 2 cut(s) 596, 612
TfiI GAWTC 1 cut(s) 670
Tru1I TTAA 2 cut(s) 120, 450
Tru9I TTAA 2 cut(s) 120, 450
TscAI CASTG 1 cut(s) 681
TseFI GTSAC 3 cut(s) 310, 334, 761
Tsp45I GTSAC 3 cut(s) 310, 334, 761
TspDTI ATGAA 3 cut(s) 17, 90, 543
TspGWI ACGGA 2 cut(s) 298, 702
TspRI CASTG 1 cut(s) 681
Tth111I GACNNNGTC 1 cut(s) 140
VpaK11BI GGWCC 2 cut(s) 391, 806
XceI RCATGY 3 cut(s) 279, 609, 784
XcmI CCANNNNNNNNNTGG 2 cut(s) 154, 279
XhoI CTCGAG 2 cut(s) 419, 485
XmnI GAANNNNTTC 1 cut(s) 61
XspI CTAG 1 cut(s) 735
ZraI GACGTC 1 cut(s) 142
ZrmI AGTACT 2 cut(s) 40, 61
Zsp2I ATGCAT 1 cut(s) 528
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.