MD14G1074600.v1.1

Protein of unknown function, DUF617

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0014282
Physical Location & Seq
Reverse (-)
8457504 .. 8457914
411 bp
Loading structure...
UTR
Exon/CDS
Intron
MD14G1074600.v1.1.491

Sequence Viewer

Length: 411 bp
ATGTCAACGTCTTCACTCGTTAAAGAGATGTCTTCGGGGCTCGTGCGCATTGCTCTCGAGTCTGAAAAGCAACAGGGTTCCGCCCGTGCAGTTATGAATGGCCGGAAGCTGTTTCAGGAGCCTTCATGGACTATGTATTGTAATGGGAGGAAGTGTGGGTATGCGGCGACTCGCACGTGCGGAGAGTCAGACTGGCACGTGCTGAGCACTGTTCGAAGTGTTTCGGTCGGAGCCGGCGTGATTCCGGTGTTGGACGACGGAAAAAAGGGTGGCGCGTCTGAAGGTGAGTTGCTTTATATGAGAGCTAGGTTTGAACGAGTTGTGGGAAGCCTTGACTCGGAGGCCTTTTACATGTTGAACCCTGAGGGTAATGGAGGTCCTGAACTCAGTATCTTTTTACTCAGAATATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF617

Protein Analysis

137

Amino Acids

14.77

Weight (kDa)

7.7

Isoelectric Point (pI)

47.3

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF617 PF04759 1 - 135 3.8e-53 Protein of unknown function, DUF617
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014282)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G41660
fragaria_vesca FvH4_6g24150
malus_domestica MD12G1076100.v1.1 MD14G1074600.v1.1
prunus_persica Prupe.7G056000_v2.0.a1
pyrus_communis pycom14g06360
rosa_chinensis RchiOBHm_Chr3g0483021
rosa_laevigata RLG00000023306
rosa_multiflora Rmu_sc0001098.1_g000001 Rmu_sc0001098.1_g000002
rosa_roxburghii Rroxscaffold_6G00398730
rosa_rugosa Rorug03G0203100
rosa_samantha Rh3AG251700 Rh3BG288200 Rh3CG285700 Rh3DG281400
rosa_wichuraiana Rw3G022770

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 47
AccII CGCG 1 cut(s) 275
AciI CCGC 3 cut(s) 81, 164, 180
AcoI YGGCCR 1 cut(s) 100
AcuI CTGAAG 1 cut(s) 300
AcvI CACGTG 2 cut(s) 177, 199
AfiI CCNNNNNNNGG 1 cut(s) 337
AflIII ACRYGT 1 cut(s) 351
AgsI TTSAA 2 cut(s) 314, 358
AluBI AGCT 2 cut(s) 109, 305
AluI AGCT 2 cut(s) 109, 305
Alw21I GWGCWC 1 cut(s) 209
Ama87I CYCGRG 1 cut(s) 56
AoxI GGCC 2 cut(s) 100, 342
Asp700I GAANNNNTTC 1 cut(s) 220
AspLEI GCGC 2 cut(s) 48, 275
AspS9I GGNCC 1 cut(s) 377
AsuHPI GGTGA 1 cut(s) 296
AsuII TTCGAA 1 cut(s) 214
AvaI CYCGRG 1 cut(s) 56
AvaII GGWCC 1 cut(s) 377
AxyI CCTNAGG 1 cut(s) 363
BanII GRGCYC 1 cut(s) 42
BauI CACGAG 1 cut(s) 41
BbrPI CACGTG 2 cut(s) 177, 199
BbsI GAAGAC 2 cut(s) 3, 24
Bbv12I GWGCWC 1 cut(s) 209
BcgI CGANNNNNNTGC 2 cut(s) 37, 71
BfaI CTAG 1 cut(s) 306
BisI GCNGC 1 cut(s) 165
BlpI GCTNAGC 1 cut(s) 203
BlsI GCNGC 1 cut(s) 166
Bme18I GGWCC 1 cut(s) 377
BmeT110I CYCGRG 1 cut(s) 56
BmgT120I GGNCC 1 cut(s) 377
BmiI GGNNCC 3 cut(s) 79, 120, 232
BpiI GAAGAC 2 cut(s) 3, 24
Bpu1102I GCTNAGC 1 cut(s) 203
Bpu14I TTCGAA 1 cut(s) 214
BsaAI YACGTR 2 cut(s) 177, 199
BsaWI WCCGGW 1 cut(s) 244
BsaXI ACNNNNNCTCC 4 cut(s) 139, 169, 222, 252
Bsc4I CCNNNNNNNGG 1 cut(s) 337
Bse118I RCCGGY 1 cut(s) 233
Bse1I ACTGG 1 cut(s) 197
Bse21I CCTNAGG 1 cut(s) 363
Bse3DI GCAATG 1 cut(s) 48
BseLI CCNNNNNNNGG 1 cut(s) 337
BseMI GCAATG 1 cut(s) 48
BseMII CTCAG 3 cut(s) 194, 354, 400
BseNI ACTGG 1 cut(s) 197
BsgI GTGCAG 1 cut(s) 108
Bsh1236I CGCG 1 cut(s) 275
Bsh1285I CGRYCG 1 cut(s) 228
BshFI GGCC 2 cut(s) 102, 344
BsiEI CGRYCG 1 cut(s) 228
BsiHKAI GWGCWC 1 cut(s) 209
BsiHKCI CYCGRG 1 cut(s) 56
BsiSI CCGG 3 cut(s) 103, 234, 245
BslI CCNNNNNNNGG 1 cut(s) 337
BsnI GGCC 2 cut(s) 102, 344
BsoBI CYCGRG 1 cut(s) 56
Bsp119I TTCGAA 1 cut(s) 214
Bsp1286I GDGCHC 2 cut(s) 42, 209
Bsp1720I GCTNAGC 1 cut(s) 203
BspACI CCGC 3 cut(s) 81, 164, 180
BspANI GGCC 2 cut(s) 102, 344
BspCNI CTCAG 3 cut(s) 195, 355, 399
BspFNI CGCG 1 cut(s) 275
BspLI GGNNCC 3 cut(s) 79, 120, 232
BspT104I TTCGAA 1 cut(s) 214
BsrDI GCAATG 1 cut(s) 48
BsrFI RCCGGY 1 cut(s) 233
BsrI ACTGG 1 cut(s) 197
BssAI RCCGGY 1 cut(s) 233
BssSI CACGAG 1 cut(s) 41
Bst2BI CACGAG 1 cut(s) 41
Bst4CI ACNGT 1 cut(s) 211
BstBAI YACGTR 2 cut(s) 177, 199
BstBI TTCGAA 1 cut(s) 214
BstC8I GCNNGC 1 cut(s) 235
BstDEI CTNAG 4 cut(s) 203, 363, 386, 401
BstFNI CGCG 1 cut(s) 275
BstHHI GCGC 2 cut(s) 48, 275
BstMCI CGRYCG 1 cut(s) 228
BstNSI RCATGY 1 cut(s) 355
BstUI CGCG 1 cut(s) 275
BstV2I GAAGAC 2 cut(s) 3, 24
Bsu36I CCTNAGG 1 cut(s) 363
BsuRI GGCC 2 cut(s) 102, 344
BtsIMutI CAGTG 1 cut(s) 207
Cac8I GCNNGC 1 cut(s) 235
CfoI GCGC 2 cut(s) 48, 275
Cfr10I RCCGGY 1 cut(s) 233
Cfr13I GGNCC 1 cut(s) 377
CseI GACGC 1 cut(s) 264
CviAII CATG 2 cut(s) 126, 352
CviJI RGCY 8 cut(s) 40, 102, 109, 121, 233, 305, 330, 344
CviKI_1 RGCY 8 cut(s) 40, 102, 109, 121, 233, 305, 330, 344
DdeI CTNAG 4 cut(s) 203, 363, 386, 401
EaeI YGGCCR 1 cut(s) 100
EciI GGCGGA 1 cut(s) 70
Eco147I AGGCCT 1 cut(s) 344
Eco24I GRGCYC 1 cut(s) 42
Eco47I GGWCC 1 cut(s) 377
Eco57I CTGAAG 1 cut(s) 300
Eco72I CACGTG 2 cut(s) 177, 199
Eco81I CCTNAGG 1 cut(s) 363
Eco88I CYCGRG 1 cut(s) 56
EcoO109I RGGNCCY 1 cut(s) 377
EcoT38I GRGCYC 1 cut(s) 42
FaeI CATG 2 cut(s) 129, 355
FaiI YATR 8 cut(s) 95, 127, 134, 162, 297, 299, 353, 409
FatI CATG 2 cut(s) 125, 351
Fnu4HI GCNGC 1 cut(s) 165
FriOI GRGCYC 1 cut(s) 42
Fsp4HI GCNGC 1 cut(s) 165
FspAI RTGCGCAY 1 cut(s) 47
FspBI CTAG 1 cut(s) 306
FspI TGCGCA 1 cut(s) 47
GlaI GCGC 2 cut(s) 47, 274
GluI GCNGC 1 cut(s) 165
HaeIII GGCC 2 cut(s) 102, 344
HapII CCGG 3 cut(s) 103, 234, 245
HgaI GACGC 1 cut(s) 264
HhaI GCGC 2 cut(s) 48, 275
Hin1II CATG 2 cut(s) 129, 355
Hin6I GCGC 2 cut(s) 46, 273
HinP1I GCGC 2 cut(s) 46, 273
HincII GTYRAC 1 cut(s) 6
HindII GTYRAC 1 cut(s) 6
HinfI GANTC 5 cut(s) 59, 169, 185, 241, 335
HpaII CCGG 3 cut(s) 103, 234, 245
HphI GGTGA 1 cut(s) 296
Hpy166II GTNNAC 1 cut(s) 6
Hpy188I TCNGA 6 cut(s) 64, 190, 230, 280, 340, 404
Hpy188III TCNNGA 3 cut(s) 56, 116, 380
Hpy8I GTNNAC 1 cut(s) 6
Hpy99I CGWCG 1 cut(s) 260
HpyAV CCTTC 2 cut(s) 132, 275
HpyCH4III ACNGT 1 cut(s) 211
HpyCH4IV ACGT 3 cut(s) 8, 176, 198
HpyCH4V TGCA 1 cut(s) 89
HpyF3I CTNAG 4 cut(s) 203, 363, 386, 401
HpySE526I ACGT 3 cut(s) 8, 176, 198
Hsp92II CATG 2 cut(s) 129, 355
HspAI GCGC 2 cut(s) 46, 273
KroI GCCGGC 1 cut(s) 233
KroNI GCCGGC 1 cut(s) 235
LmnI GCTCC 2 cut(s) 118, 230
LpnPI CCDG 8 cut(s) 59, 101, 116, 178, 247, 258, 375, 393
MaeI CTAG 1 cut(s) 306
MaeII ACGT 3 cut(s) 8, 176, 198
MboII GAAGA 2 cut(s) 3, 24
MhlI GDGCHC 2 cut(s) 42, 209
MlyI GAGTC 4 cut(s) 68, 163, 194, 329
MmeI TCCRAC 2 cut(s) 208, 231
MnlI CCTC 4 cut(s) 141, 334, 358, 368
MroNI GCCGGC 1 cut(s) 233
MroXI GAANNNNTTC 1 cut(s) 220
MseI TTAA 1 cut(s) 21
MspI CCGG 3 cut(s) 103, 234, 245
MvnI CGCG 1 cut(s) 275
NaeI GCCGGC 1 cut(s) 235
NgoMIV GCCGGC 1 cut(s) 233
NlaIII CATG 2 cut(s) 129, 355
NlaIV GGNNCC 3 cut(s) 79, 120, 232
NsbI TGCGCA 1 cut(s) 47
NspI RCATGY 1 cut(s) 355
NspV TTCGAA 1 cut(s) 214
PaeR7I CTCGAG 1 cut(s) 56
PceI AGGCCT 1 cut(s) 344
PciI ACATGT 1 cut(s) 351
PcsI WCGNNNNNNNCGW 1 cut(s) 234
PdiI GCCGGC 1 cut(s) 235
PdmI GAANNNNTTC 1 cut(s) 220
PfeI GAWTC 1 cut(s) 241
PkrI GCNGC 1 cut(s) 166
PleI GAGTC 4 cut(s) 67, 163, 193, 329
PmaCI CACGTG 2 cut(s) 177, 199
PmlI CACGTG 2 cut(s) 177, 199
PpsI GAGTC 4 cut(s) 67, 163, 193, 329
Ppu21I YACGTR 2 cut(s) 177, 199
PpuMI RGGWCCY 1 cut(s) 377
PscI ACATGT 1 cut(s) 351
Psp5II RGGWCCY 1 cut(s) 377
PspCI CACGTG 2 cut(s) 177, 199
PspN4I GGNNCC 3 cut(s) 79, 120, 232
PspPI GGNCC 1 cut(s) 377
PspPPI RGGWCCY 1 cut(s) 377
SaqAI TTAA 1 cut(s) 21
SatI GCNGC 1 cut(s) 165
Sau96I GGNCC 1 cut(s) 377
SchI GAGTC 4 cut(s) 68, 163, 194, 329
SduI GDGCHC 2 cut(s) 42, 209
SetI ASST 8 cut(s) 11, 111, 179, 201, 286, 307, 311, 379
Sfr274I CTCGAG 1 cut(s) 56
SfuI TTCGAA 1 cut(s) 214
SinI GGWCC 1 cut(s) 377
SlaI CTCGAG 1 cut(s) 56
SmlI CTYRAG 1 cut(s) 56
SmoI CTYRAG 1 cut(s) 56
SseBI AGGCCT 1 cut(s) 344
SsiI CCGC 3 cut(s) 81, 164, 180
SspMI CTAG 1 cut(s) 306
StuI AGGCCT 1 cut(s) 344
TaaI ACNGT 1 cut(s) 211
TaiI ACGT 3 cut(s) 11, 179, 201
TaqI TCGA 2 cut(s) 57, 214
TaqII GACCGA 1 cut(s) 214
TauI GCSGC 1 cut(s) 167
TfiI GAWTC 1 cut(s) 241
Tru1I TTAA 1 cut(s) 21
Tru9I TTAA 1 cut(s) 21
TscAI CASTG 1 cut(s) 214
TspDTI ATGAA 2 cut(s) 110, 114
TspGWI ACGGA 1 cut(s) 273
TspRI CASTG 1 cut(s) 214
VpaK11BI GGWCC 1 cut(s) 377
XceI RCATGY 1 cut(s) 355
XhoI CTCGAG 1 cut(s) 56
XmnI GAANNNNTTC 1 cut(s) 220
XspI CTAG 1 cut(s) 306
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.