Rroxscaffold_6G00398730

Protein of unknown function, DUF617

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000006
OG0014282
Physical Location & Seq
Forward (+)
20623352 .. 20624215
864 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_6G00398730.1

Sequence Viewer

Length: 864 bp
ATGAACCTCCAAAGAAGCAACAGCATTAGTACTAGCTCCAACACAACTAGAAAAATCGTCCCTTCAAACCAGTTCATCAGATCCTCTTCAACAGCTCTGACACCACCTCAACATCATCACCACCGCCGTCGCCAAAACGACGACTTCTCCAACAAGCTACTGATTCCCAAACACAACTCTTCATCGTCCCCGGTCTCTTTCGACCACCTCTCCAAAACCCAGACAAAGATCTCCTCACTCCTCCGCTCTTTTCTCAACCTCATCTCCTTCCCAGCCGCCATTATCAACCTCCCCGCCAGCTGCAAGTGGCTCGCCATCTCTTCCCATCTCTCGGTCACTCCCTCTCTCGGCCGGAAAGTCACCGGAACCCTATTTGGTAACCGCCGCGGCCACGTCAGCTTCGCCGTCCAGCTCGACCCCAGGTCCGAGCCCGTCTTGCTTCTCGAGCTGGCCATGTCCACGTCTTCCCTCGTTAAGGAGATGTCTTCCGGACTCGTCCGCATTGCTCTCGAGTCCGAAAGGCATCAGGGTTCCGGCCGGAAGCCTTTGTTTCACGAGCCTACCTGGACCATGTACTGCAATGGGAGGAAGTTGGGGTACGCGGCGTCTCGCACATGCGGAGAGTCCGACTGGCACGTGCTGAATACTGTCCGGAGCGTTTCAGTTGGAGCCGGGGTGATTCCGGTGGTGGACGACGGGAAAGTGGGTAGCGGCGGGTCGTCGGAGGGTTTAGGCGAGTTGCTTTACATGAGGGCTCGGTTTGAACGAGTTGTGGGGAGCCGTGACTCGGAGGCCTTCTACATGTTGAATCCTGATGGGAGTAATGGAGGTCCTGAACTCAGCATCTTCTTGCTTAGAATATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF617

Protein Analysis

287

Amino Acids

31.43

Weight (kDa)

10.06

Isoelectric Point (pI)

54.76

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF617 PF04759 120 - 286 6.5e-69 Protein of unknown function, DUF617
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014282)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G41660
fragaria_vesca FvH4_6g24150
malus_domestica MD12G1076100.v1.1 MD14G1074600.v1.1
prunus_persica Prupe.7G056000_v2.0.a1
pyrus_communis pycom14g06360
rosa_chinensis RchiOBHm_Chr3g0483021
rosa_laevigata RLG00000023306
rosa_multiflora Rmu_sc0001098.1_g000001 Rmu_sc0001098.1_g000002
rosa_roxburghii Rroxscaffold_6G00398730
rosa_rugosa Rorug03G0203100
rosa_samantha Rh3AG251700 Rh3BG288200 Rh3CG285700 Rh3DG281400
rosa_wichuraiana Rw3G022770

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 246
AccII CGCG 2 cut(s) 387, 602
AccIII TCCGGA 2 cut(s) 488, 651
AclWI GGATC 1 cut(s) 75
AcoI YGGCCR 4 cut(s) 349, 388, 450, 535
AcvI CACGTG 1 cut(s) 637
AcyI GRCGYC 1 cut(s) 605
AfaI GTAC 3 cut(s) 31, 575, 599
AfiI CCNNNNNNNGG 4 cut(s) 331, 347, 475, 787
AflIII ACRYGT 1 cut(s) 801
AgsI TTSAA 4 cut(s) 66, 90, 764, 808
AhdI GACNNNNNGTC 1 cut(s) 421
AjiI CACGTC 2 cut(s) 394, 462
AjnI CCWGG 2 cut(s) 419, 563
AluBI AGCT 7 cut(s) 36, 95, 157, 300, 399, 412, 448
AluI AGCT 7 cut(s) 36, 95, 157, 300, 399, 412, 448
Alw26I GTCTC 2 cut(s) 199, 612
AlwI GGATC 1 cut(s) 75
Ama87I CYCGRG 2 cut(s) 443, 509
Aor13HI TCCGGA 2 cut(s) 488, 651
AoxI GGCC 5 cut(s) 349, 388, 450, 535, 792
ApeKI GCWGC 1 cut(s) 300
AspS9I GGNCC 3 cut(s) 423, 567, 830
AsuC2I CCSGG 2 cut(s) 191, 673
AsuHPI GGTGA 3 cut(s) 110, 352, 688
AvaI CYCGRG 2 cut(s) 443, 509
AvaII GGWCC 3 cut(s) 423, 567, 830
BalI TGGCCA 1 cut(s) 452
BanII GRGCYC 2 cut(s) 432, 757
BarI GAAGNNNNNNTAC 2 cut(s) 581, 613
BauI CACGAG 1 cut(s) 554
BbrPI CACGTG 1 cut(s) 637
BbsI GAAGAC 2 cut(s) 456, 477
BbvI GCAGC 1 cut(s) 287
BccI CCATC 3 cut(s) 323, 333, 809
BceAI ACGGC 3 cut(s) 111, 389, 765
BcgI CGANNNNNNTGC 4 cut(s) 292, 326, 490, 524
BciT130I CCWGG 2 cut(s) 421, 565
BcnI CCSGG 2 cut(s) 191, 673
BcoDI GTCTC 2 cut(s) 199, 612
BfaI CTAG 2 cut(s) 33, 48
BglII AGATCT 1 cut(s) 228
BisI GCNGC 6 cut(s) 276, 301, 385, 388, 603, 712
BlsI GCNGC 6 cut(s) 277, 302, 386, 389, 604, 713
BmcAI AGTACT 1 cut(s) 31
Bme1390I CCNGG 4 cut(s) 191, 421, 565, 673
Bme18I GGWCC 3 cut(s) 423, 567, 830
BmeRI GACNNNNNGTC 1 cut(s) 421
BmeT110I CYCGRG 2 cut(s) 443, 509
BmgBI CACGTC 2 cut(s) 394, 462
BmgT120I GGNCC 3 cut(s) 423, 567, 830
BmiI GGNNCC 4 cut(s) 367, 532, 670, 779
BmrFI CCNGG 4 cut(s) 191, 421, 565, 673
BmsI GCATC 2 cut(s) 532, 852
BpiI GAAGAC 2 cut(s) 456, 477
BpuMI CCSGG 2 cut(s) 191, 673
BsaAI YACGTR 1 cut(s) 637
BsaHI GRCGYC 1 cut(s) 605
BsaI GGTCTC 1 cut(s) 199
BsaJI CCNNGG 4 cut(s) 189, 385, 419, 672
BsaWI WCCGGW 4 cut(s) 362, 488, 651, 682
BsaXI ACNNNNNCTCC 8 cut(s) 131, 161, 194, 224, 248, 278, 660, 690
Bsc4I CCNNNNNNNGG 4 cut(s) 331, 347, 475, 787
Bse1I ACTGG 2 cut(s) 70, 635
Bse3DI GCAATG 2 cut(s) 501, 586
BseAI TCCGGA 2 cut(s) 488, 651
BseBI CCWGG 2 cut(s) 421, 565
BseDI CCNNGG 4 cut(s) 189, 385, 419, 672
BseLI CCNNNNNNNGG 4 cut(s) 331, 347, 475, 787
BseMI GCAATG 2 cut(s) 501, 586
BseMII CTCAG 1 cut(s) 853
BseNI ACTGG 2 cut(s) 70, 635
BseRI GAGGAG 2 cut(s) 223, 230
BseX3I CGGCCG 2 cut(s) 349, 535
BseXI GCAGC 1 cut(s) 287
BseYI CCCAGC 1 cut(s) 271
Bsh1236I CGCG 2 cut(s) 387, 602
Bsh1285I CGRYCG 2 cut(s) 352, 538
BshFI GGCC 5 cut(s) 351, 390, 452, 537, 794
BsiEI CGRYCG 2 cut(s) 352, 538
BsiHKCI CYCGRG 2 cut(s) 443, 509
BsiSI CCGG 9 cut(s) 191, 352, 363, 489, 534, 538, 652, 672, 683
BslFI GGGAC 2 cut(s) 44, 172
BslI CCNNNNNNNGG 4 cut(s) 331, 347, 475, 787
BsmAI GTCTC 2 cut(s) 199, 612
BsmBI CGTCTC 1 cut(s) 612
BsmFI GGGAC 2 cut(s) 44, 172
BsnI GGCC 5 cut(s) 351, 390, 452, 537, 794
Bso31I GGTCTC 1 cut(s) 199
BsoBI CYCGRG 2 cut(s) 443, 509
Bsp1286I GDGCHC 2 cut(s) 432, 757
Bsp13I TCCGGA 2 cut(s) 488, 651
Bsp143I GATC 2 cut(s) 80, 228
BspANI GGCC 5 cut(s) 351, 390, 452, 537, 794
BspCNI CTCAG 1 cut(s) 852
BspEI TCCGGA 2 cut(s) 488, 651
BspFNI CGCG 2 cut(s) 387, 602
BspLI GGNNCC 4 cut(s) 367, 532, 670, 779
BspPI GGATC 1 cut(s) 75
BspTNI GGTCTC 1 cut(s) 199
BsrBI CCGCTC 1 cut(s) 246
BsrDI GCAATG 2 cut(s) 501, 586
BsrI ACTGG 2 cut(s) 70, 635
BssECI CCNNGG 4 cut(s) 189, 385, 419, 672
BssMI GATC 2 cut(s) 80, 228
BssNI GRCGYC 1 cut(s) 605
BssSI CACGAG 1 cut(s) 554
Bst2BI CACGAG 1 cut(s) 554
Bst2UI CCWGG 2 cut(s) 421, 565
Bst4CI ACNGT 1 cut(s) 649
Bst6I CTCTTC 3 cut(s) 91, 184, 325
BstACI GRCGYC 1 cut(s) 605
BstBAI YACGTR 1 cut(s) 637
BstC8I GCNNGC 3 cut(s) 298, 312, 450
BstDEI CTNAG 2 cut(s) 839, 854
BstDSI CCRYGG 1 cut(s) 385
BstEII GGTNACC 1 cut(s) 377
BstENI CCTNNNNNAGG 1 cut(s) 473
BstFNI CGCG 2 cut(s) 387, 602
BstKTI GATC 2 cut(s) 83, 231
BstMAI GTCTC 2 cut(s) 199, 612
BstMBI GATC 2 cut(s) 80, 228
BstMCI CGRYCG 2 cut(s) 352, 538
BstMWI GCNNNNNNNGC 3 cut(s) 396, 436, 445
BstNI CCWGG 2 cut(s) 421, 565
BstNSI RCATGY 2 cut(s) 618, 805
BstPI GGTNACC 1 cut(s) 377
BstSCI CCNGG 4 cut(s) 189, 419, 563, 671
BstUI CGCG 2 cut(s) 387, 602
BstV1I GCAGC 1 cut(s) 287
BstV2I GAAGAC 2 cut(s) 456, 477
BstX2I RGATCY 2 cut(s) 80, 228
BstYI RGATCY 2 cut(s) 80, 228
BstZI CGGCCG 2 cut(s) 349, 535
BsuRI GGCC 5 cut(s) 351, 390, 452, 537, 794
BtgI CCRYGG 1 cut(s) 385
BtrI CACGTC 2 cut(s) 394, 462
Cac8I GCNNGC 3 cut(s) 298, 312, 450
Cfr13I GGNCC 3 cut(s) 423, 567, 830
Cfr42I CCGCGG 1 cut(s) 388
CseI GACGC 1 cut(s) 594
Csp6I GTAC 3 cut(s) 30, 574, 598
CviAII CATG 5 cut(s) 454, 571, 615, 748, 802
CviQI GTAC 3 cut(s) 30, 574, 598
DdeI CTNAG 2 cut(s) 839, 854
DpnI GATC 2 cut(s) 82, 230
DpnII GATC 2 cut(s) 80, 228
DriI GACNNNNNGTC 1 cut(s) 421
EaeI YGGCCR 4 cut(s) 349, 388, 450, 535
EagI CGGCCG 2 cut(s) 349, 535
Eam1104I CTCTTC 3 cut(s) 91, 184, 325
Eam1105I GACNNNNNGTC 1 cut(s) 421
EarI CTCTTC 3 cut(s) 91, 184, 325
EclXI CGGCCG 2 cut(s) 349, 535
Eco147I AGGCCT 1 cut(s) 794
Eco24I GRGCYC 2 cut(s) 432, 757
Eco31I GGTCTC 1 cut(s) 199
Eco47I GGWCC 3 cut(s) 423, 567, 830
Eco52I CGGCCG 2 cut(s) 349, 535
Eco72I CACGTG 1 cut(s) 637
Eco88I CYCGRG 2 cut(s) 443, 509
Eco91I GGTNACC 1 cut(s) 377
EcoNI CCTNNNNNAGG 1 cut(s) 473
EcoO109I RGGNCCY 1 cut(s) 830
EcoO65I GGTNACC 1 cut(s) 377
EcoRII CCWGG 2 cut(s) 419, 563
EcoT38I GRGCYC 2 cut(s) 432, 757
Esp3I CGTCTC 1 cut(s) 612
FaeI CATG 5 cut(s) 457, 574, 618, 751, 805
FaiI YATR 6 cut(s) 455, 572, 616, 749, 803, 862
FaqI GGGAC 2 cut(s) 44, 172
FatI CATG 5 cut(s) 453, 570, 614, 747, 801
FauI CCCGC 2 cut(s) 301, 707
Fnu4HI GCNGC 6 cut(s) 276, 301, 385, 388, 603, 712
FriOI GRGCYC 2 cut(s) 432, 757
Fsp4HI GCNGC 6 cut(s) 276, 301, 385, 388, 603, 712
FspBI CTAG 2 cut(s) 33, 48
GluI GCNGC 6 cut(s) 276, 301, 385, 388, 603, 712
GsaI CCCAGC 1 cut(s) 275
HaeIII GGCC 5 cut(s) 351, 390, 452, 537, 794
HapII CCGG 9 cut(s) 191, 352, 363, 489, 534, 538, 652, 672, 683
HgaI GACGC 1 cut(s) 594
Hin1I GRCGYC 1 cut(s) 605
Hin1II CATG 5 cut(s) 457, 574, 618, 751, 805
HinfI GANTC 7 cut(s) 163, 492, 512, 623, 679, 785, 808
HpaII CCGG 9 cut(s) 191, 352, 363, 489, 534, 538, 652, 672, 683
HphI GGTGA 3 cut(s) 110, 352, 688
Hpy166II GTNNAC 2 cut(s) 459, 691
Hpy188I TCNGA 7 cut(s) 80, 99, 427, 517, 628, 724, 790
Hpy188III TCNNGA 7 cut(s) 443, 489, 509, 554, 652, 812, 833
Hpy8I GTNNAC 2 cut(s) 459, 691
Hpy99I CGWCG 4 cut(s) 132, 143, 698, 724
HpyAV CCTTC 3 cut(s) 72, 277, 805
HpyCH4III ACNGT 1 cut(s) 649
HpyCH4IV ACGT 3 cut(s) 393, 461, 636
HpyCH4V TGCA 2 cut(s) 303, 579
HpyF10VI GCNNNNNNNGC 3 cut(s) 396, 436, 445
HpyF3I CTNAG 2 cut(s) 839, 854
HpySE526I ACGT 3 cut(s) 393, 461, 636
Hsp92I GRCGYC 1 cut(s) 605
Hsp92II CATG 5 cut(s) 457, 574, 618, 751, 805
Kpn2I TCCGGA 2 cut(s) 488, 651
KspI CCGCGG 1 cut(s) 388
Kzo9I GATC 2 cut(s) 80, 228
LmnI GCTCC 4 cut(s) 41, 654, 668, 777
Lsp1109I GCAGC 1 cut(s) 287
LweI GCATC 2 cut(s) 532, 852
MaeI CTAG 2 cut(s) 33, 48
MaeII ACGT 3 cut(s) 393, 461, 636
MaeIII GTNAC 4 cut(s) 334, 358, 377, 782
MalI GATC 2 cut(s) 82, 230
MbiI CCGCTC 1 cut(s) 246
MboI GATC 2 cut(s) 80, 228
MboII GAAGA 6 cut(s) 78, 171, 312, 456, 477, 838
MflI RGATCY 2 cut(s) 80, 228
MhlI GDGCHC 2 cut(s) 432, 757
MlsI TGGCCA 1 cut(s) 452
MluNI TGGCCA 1 cut(s) 452
MlyI GAGTC 4 cut(s) 486, 521, 632, 779
MmeI TCCRAC 5 cut(s) 63, 174, 646, 651, 702
Mox20I TGGCCA 1 cut(s) 452
MroI TCCGGA 2 cut(s) 488, 651
MscI TGGCCA 1 cut(s) 452
MseI TTAA 1 cut(s) 474
Msp20I TGGCCA 1 cut(s) 452
MspA1I CMGCKG 2 cut(s) 300, 387
MspI CCGG 9 cut(s) 191, 352, 363, 489, 534, 538, 652, 672, 683
MspR9I CCNGG 4 cut(s) 191, 421, 565, 673
MvaI CCWGG 2 cut(s) 421, 565
MvnI CGCG 2 cut(s) 387, 602
MwoI GCNNNNNNNGC 3 cut(s) 396, 436, 445
NciI CCSGG 2 cut(s) 191, 673
NdeII GATC 2 cut(s) 80, 228
NlaIII CATG 5 cut(s) 457, 574, 618, 751, 805
NlaIV GGNNCC 4 cut(s) 367, 532, 670, 779
NmeAIII GCCGAG 1 cut(s) 327
NmuCI GTSAC 3 cut(s) 334, 358, 782
NspI RCATGY 2 cut(s) 618, 805
PaeR7I CTCGAG 2 cut(s) 443, 509
PceI AGGCCT 1 cut(s) 794
PciI ACATGT 1 cut(s) 801
PcsI WCGNNNNNNNCGW 1 cut(s) 763
PfeI GAWTC 3 cut(s) 163, 679, 808
PkrI GCNGC 6 cut(s) 277, 302, 386, 389, 604, 713
PleI GAGTC 4 cut(s) 486, 520, 631, 779
PmaCI CACGTG 1 cut(s) 637
PmlI CACGTG 1 cut(s) 637
PpsI GAGTC 4 cut(s) 486, 520, 631, 779
Ppu21I YACGTR 1 cut(s) 637
PpuMI RGGWCCY 1 cut(s) 830
PscI ACATGT 1 cut(s) 801
Psp5II RGGWCCY 1 cut(s) 830
Psp6I CCWGG 2 cut(s) 419, 563
PspCI CACGTG 1 cut(s) 637
PspEI GGTNACC 1 cut(s) 377
PspFI CCCAGC 1 cut(s) 271
PspGI CCWGG 2 cut(s) 419, 563
PspN4I GGNNCC 4 cut(s) 367, 532, 670, 779
PspPI GGNCC 3 cut(s) 423, 567, 830
PspPPI RGGWCCY 1 cut(s) 830
PsuI RGATCY 2 cut(s) 80, 228
PvuII CAGCTG 1 cut(s) 300
RsaI GTAC 3 cut(s) 31, 575, 599
RsaNI GTAC 3 cut(s) 30, 574, 598
SacII CCGCGG 1 cut(s) 388
SaqAI TTAA 1 cut(s) 474
SatI GCNGC 6 cut(s) 276, 301, 385, 388, 603, 712
Sau3AI GATC 2 cut(s) 80, 228
Sau96I GGNCC 3 cut(s) 423, 567, 830
ScaI AGTACT 1 cut(s) 31
SchI GAGTC 4 cut(s) 486, 521, 632, 779
ScrFI CCNGG 4 cut(s) 191, 421, 565, 673
SduI GDGCHC 2 cut(s) 432, 757
SfaNI GCATC 2 cut(s) 532, 852
Sfr274I CTCGAG 2 cut(s) 443, 509
Sfr303I CCGCGG 1 cut(s) 388
SgrBI CCGCGG 1 cut(s) 388
SinI GGWCC 3 cut(s) 423, 567, 830
SlaI CTCGAG 2 cut(s) 443, 509
SmlI CTYRAG 2 cut(s) 443, 509
SmoI CTYRAG 2 cut(s) 443, 509
SseBI AGGCCT 1 cut(s) 794
SspMI CTAG 2 cut(s) 33, 48
StuI AGGCCT 1 cut(s) 794
StyD4I CCNGG 4 cut(s) 189, 419, 563, 671
TaaI ACNGT 1 cut(s) 649
TaiI ACGT 3 cut(s) 396, 464, 639
TaqI TCGA 4 cut(s) 201, 414, 444, 510
TaqII GACCGA 1 cut(s) 322
TatI WGTACW 2 cut(s) 29, 573
TauI GCSGC 5 cut(s) 278, 387, 390, 605, 714
TfiI GAWTC 3 cut(s) 163, 679, 808
Tru1I TTAA 1 cut(s) 474
Tru9I TTAA 1 cut(s) 474
TseFI GTSAC 3 cut(s) 334, 358, 782
TseI GCWGC 1 cut(s) 300
Tsp45I GTSAC 3 cut(s) 334, 358, 782
TspDTI ATGAA 3 cut(s) 17, 64, 171
VpaK11BI GGWCC 3 cut(s) 423, 567, 830
XagI CCTNNNNNAGG 1 cut(s) 473
XceI RCATGY 2 cut(s) 618, 805
XhoI CTCGAG 2 cut(s) 443, 509
XspI CTAG 2 cut(s) 33, 48
ZrmI AGTACT 1 cut(s) 31
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.