AT2G45310

4-epimerase 4

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
2
OG0015857
Physical Location & Seq
Forward (+)
18682081 .. 18684042
1962 bp
Loading structure...
UTR
Exon/CDS
Intron
AT2G45310.1

Sequence Viewer

Length: 1314 bp
ATGTCACGTCTTGACGACATACCTTCAAGCCCAGGCAAATTCAAGATGGAGAAATCTTCCTACTTGCATCGTCTCAGATTCCAATCTTCTCTCACAAAGTTTGCATTTTTCTCCTTCTTCCTCCTCTGTCTCATCTCTCTACTCTTCCTCCGATCTCCACCGTCCATTAACCCTTCTTCACCGTCCGATCCATCTCGCCGTTCACTCCGAACCAACACCTACGGAGGACCAGCTTGGGAAAAACGTCTCAGATCCTCAGCTCGAATCCGAACCTCAACAAACAACGGAATCACAGTTTTAGTAACCGGAGCAGCTGGTTTCGTCGGAACACACGTCTCCGCCGCATTAAAACGCCGCGGAGACGGCGTAATCGGCCTCGACAACTTCAACGATTACTACGATCCATCTCTGAAACGAGCAAGACGAGCTTTACTCGAGAGAAGTGGGATCTTCATCGTAGAAGGAGACATAAACGACGTCGAATTACTCCGTAAGCTATTCAAGATCGTCTCCTTCACACACGTTATGCATTTAGCAGCTCAAGCTGGTGTACGGTACGCAATGGAAAACCCTAGCTCGTACGTTCATAGCAACATCGCCGGATTCGTCAATCTCCTCGAGATCTGCAAATCTGTGAATCCACAACCTGCTATAGTCTGGGCTTCGTCTAGCTCCGTCTACGGACTCAACACCAAAGTTCCGTTCTCGGAGAAAGATAAAACAGATCAACCGGCGAGTTTATACGCCGCCACTAAAAAAGCCGGCGAAGAGATCGCACATACCTACAACCACATCTATGGATTATCTCTAACCGGATTAAGATTTTTCACCGTGTATGGACCTTGGGGAAGACCAGACATGGCGTATTTCTTCTTCACTAAAGATATTCTAAAAGGCAAATCGATTTCGATATTCGAATCGGCGAATCACGGAACGGTGGCTAGGGATTTCACTTACATAGACGATATAGTGAAGGGATGTTTAGCGGCGCTAGATACGGCGGAGAAGAGTACAGGGAGTGGAGGGAAGAAGAGAGGTCCGGCGCAATTGAGAGTGTTTAACCTCGGAAACACGTCGCCGGTGCCGGTTTCAGATCTGGTGAGGATATTGGAGAGACAATTGAAAGTGAAGGCGAAGAAGAATTTGATTAAGATGCCACGAAACGGTGACGTTCCGTTTACTCATGCTAATATTAGTTTAGCTCAACGTGAGCTTGGTTATAAACCGACTACGGATTTGCAAACCGGTTTGAAGAAGTTTGTCAGATGGTATTTGAGTTATTACTCCGGCGACAAAAAAGCCGCCGCTAGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0016020
KEGG Pathways
Metabolic & Signaling
ko00520 ko01100 map00520 map01100
Pfam Domains
Protein Families
Epimerase GDP_Man_Dehyd

Protein Analysis

437

Amino Acids

48.54

Weight (kDa)

9.95

Isoelectric Point (pI)

47.8

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RmlD_sub_bind PF04321 98 - 281 1.1e-14 RmlD substrate binding domain
Epimerase PF01370 99 - 336 1e-52 NAD dependent epimerase/dehydratase family
Polysacc_synt_2 PF02719 99 - 334 6e-10 Polysaccharide biosynthesis protein
GDP_Man_Dehyd PF16363 100 - 419 2.3e-48 GDP-mannose 4,6 dehydratase
3Beta_HSD PF01073 100 - 381 4.5e-14 3-beta hydroxysteroid dehydrogenase/isomerase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015857)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G45310 AT4G12250
fragaria_vesca FvH4_5g00620
prunus_persica Prupe.5G079000_v2.0.a1
rosa_chinensis RchiOBHm_Chr7g0201641
rosa_laevigata RLG00000003659
rosa_multiflora Rmu_sc0031046.1_g000001 Rmu_sc0031047.1_g000001
rosa_roxburghii Rroxscaffold_3G00255170
rosa_rugosa Rorug07G0065300
rosa_samantha Rh7AG192100 Rh7BG194300 Rh7CG203400 Rh7DG197400
rosa_wichuraiana Rw7G016850

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1221
AatII GACGTC 1 cut(s) 480
Acc36I ACCTGC 1 cut(s) 655
AccB1I GGYRCC 1 cut(s) 1081
AccI GTMKAC 1 cut(s) 678
AccII CGCG 1 cut(s) 357
AciI CCGC 9 cut(s) 339, 342, 355, 357, 747, 986, 1001, 1302, 1305
AclWI GGATC 4 cut(s) 182, 246, 395, 455
AcsI RAATTY 2 cut(s) 38, 1141
AcyI GRCGYC 1 cut(s) 477
AfaI GTAC 4 cut(s) 552, 557, 581, 1012
AfiI CCNNNNNNNGG 1 cut(s) 1163
AflIII ACRYGT 3 cut(s) 331, 520, 1071
AgeI ACCGGT 1 cut(s) 1244
AgsI TTSAA 6 cut(s) 27, 43, 388, 502, 1123, 1252
AjiI CACGTC 3 cut(s) 8, 334, 1074
AjnI CCWGG 1 cut(s) 31
AjuI GAANNNNNNNTTGG 2 cut(s) 686, 718
Alw26I GTCTC 8 cut(s) 77, 134, 251, 340, 354, 459, 514, 1108
AlwI GGATC 4 cut(s) 182, 246, 395, 455
Ama87I CYCGRG 2 cut(s) 434, 617
AoxI GGCC 1 cut(s) 373
ApeKI GCWGC 2 cut(s) 311, 536
ApoI RAATTY 2 cut(s) 38, 1141
ArsI GACNNNNNNTTYG 2 cut(s) 1219, 1251
AsiGI ACCGGT 1 cut(s) 1244
AspLEI GCGC 2 cut(s) 991, 1045
AspS9I GGNCC 3 cut(s) 227, 839, 1037
AsuHPI GGTGA 4 cut(s) 171, 820, 1111, 1178
AsuII TTCGAA 1 cut(s) 915
AvaI CYCGRG 2 cut(s) 434, 617
AvaII GGWCC 3 cut(s) 227, 839, 1037
BanI GGYRCC 1 cut(s) 1081
BarI GAAGNNNNNNTAC 2 cut(s) 857, 889
BbsI GAAGAC 1 cut(s) 856
BbvCI CCTCAGC 1 cut(s) 256
BbvI GCAGC 2 cut(s) 323, 548
BccI CCATC 4 cut(s) 40, 199, 412, 1260
BceAI ACGGC 3 cut(s) 183, 379, 1014
BciT130I CCWGG 1 cut(s) 33
BcoDI GTCTC 8 cut(s) 77, 134, 251, 340, 354, 459, 514, 1108
BfaI CTAG 5 cut(s) 573, 669, 942, 992, 1308
BfmI CTRYAG 1 cut(s) 651
BfoI RGCGCY 1 cut(s) 992
BfuAI ACCTGC 1 cut(s) 655
BglII AGATCT 2 cut(s) 621, 1093
BisI GCNGC 8 cut(s) 312, 342, 355, 537, 747, 987, 1302, 1305
BlsI GCNGC 8 cut(s) 313, 343, 356, 538, 748, 988, 1303, 1306
Bme1390I CCNGG 1 cut(s) 33
Bme18I GGWCC 3 cut(s) 227, 839, 1037
BmeT110I CYCGRG 2 cut(s) 434, 617
BmgBI CACGTC 3 cut(s) 8, 334, 1074
BmgT120I GGNCC 3 cut(s) 227, 839, 1037
BmiI GGNNCC 1 cut(s) 1083
BmrFI CCNGG 1 cut(s) 33
BmsI GCATC 2 cut(s) 76, 1143
BpiI GAAGAC 1 cut(s) 856
BplI GAGNNNNNCTC 2 cut(s) 417, 449
Bpu10I CCTNAGC 1 cut(s) 256
Bpu14I TTCGAA 1 cut(s) 915
BpuEI CTTGAG 1 cut(s) 525
Bsa29I ATCGAT 1 cut(s) 902
BsaBI GATNNNNATC 2 cut(s) 82, 452
BsaHI GRCGYC 1 cut(s) 477
BsaJI CCNNGG 4 cut(s) 31, 355, 842, 1063
BsaWI WCCGGW 3 cut(s) 305, 812, 1244
BsaXI ACNNNNNCTCC 4 cut(s) 132, 162, 351, 381
Bsc4I CCNNNNNNNGG 1 cut(s) 1163
Bse118I RCCGGY 5 cut(s) 730, 761, 1078, 1084, 1244
Bse3DI GCAATG 1 cut(s) 567
Bse8I GATNNNNATC 2 cut(s) 82, 452
BseBI CCWGG 1 cut(s) 33
BseCI ATCGAT 1 cut(s) 902
BseDI CCNNGG 4 cut(s) 31, 355, 842, 1063
BseGI GGATG 1 cut(s) 983
BseJI GATNNNNATC 2 cut(s) 82, 452
BseLI CCNNNNNNNGG 1 cut(s) 1163
BseMI GCAATG 1 cut(s) 567
BseMII CTCAG 3 cut(s) 88, 262, 270
BseRI GAGGAG 2 cut(s) 113, 605
BseXI GCAGC 2 cut(s) 323, 548
Bsh1236I CGCG 1 cut(s) 357
BshFI GGCC 1 cut(s) 375
BshNI GGYRCC 1 cut(s) 1081
BshTI ACCGGT 1 cut(s) 1244
BshVI ATCGAT 1 cut(s) 902
BsiHKCI CYCGRG 2 cut(s) 434, 617
BsiWI CGTACG 1 cut(s) 579
BslI CCNNNNNNNGG 1 cut(s) 1163
BsmAI GTCTC 8 cut(s) 77, 134, 251, 340, 354, 459, 514, 1108
BsmBI CGTCTC 5 cut(s) 77, 251, 340, 354, 514
BsnI GGCC 1 cut(s) 375
BsoBI CYCGRG 2 cut(s) 434, 617
Bsp119I TTCGAA 1 cut(s) 915
BspACI CCGC 9 cut(s) 339, 342, 355, 357, 747, 986, 1001, 1302, 1305
BspANI GGCC 1 cut(s) 375
BspCNI CTCAG 3 cut(s) 87, 261, 269
BspDI ATCGAT 1 cut(s) 902
BspFNI CGCG 1 cut(s) 357
BspLI GGNNCC 1 cut(s) 1083
BspMI ACCTGC 1 cut(s) 655
BspPI GGATC 4 cut(s) 182, 246, 395, 455
BspT104I TTCGAA 1 cut(s) 915
BspT107I GGYRCC 1 cut(s) 1081
BsrDI GCAATG 1 cut(s) 567
BsrFI RCCGGY 5 cut(s) 730, 761, 1078, 1084, 1244
BssAI RCCGGY 5 cut(s) 730, 761, 1078, 1084, 1244
BssECI CCNNGG 4 cut(s) 31, 355, 842, 1063
BssNI GRCGYC 1 cut(s) 477
BssT1I CCWWGG 1 cut(s) 842
Bst2UI CCWGG 1 cut(s) 33
Bst4CI ACNGT 7 cut(s) 162, 183, 295, 555, 832, 937, 1166
Bst6I CTCTTC 4 cut(s) 149, 762, 1001, 1025
BstACI GRCGYC 1 cut(s) 477
BstBI TTCGAA 1 cut(s) 915
BstC8I GCNNGC 1 cut(s) 763
BstDEI CTNAG 3 cut(s) 74, 248, 256
BstDSI CCRYGG 1 cut(s) 355
BstF5I GGATG 1 cut(s) 983
BstFNI CGCG 1 cut(s) 357
BstH2I RGCGCY 1 cut(s) 992
BstHHI GCGC 2 cut(s) 991, 1045
BstMAI GTCTC 8 cut(s) 77, 134, 251, 340, 354, 459, 514, 1108
BstMWI GCNNNNNNNGC 4 cut(s) 363, 372, 425, 542
BstNI CCWGG 1 cut(s) 33
BstSCI CCNGG 1 cut(s) 31
BstSFI CTRYAG 1 cut(s) 651
BstUI CGCG 1 cut(s) 357
BstV1I GCAGC 2 cut(s) 323, 548
BstV2I GAAGAC 1 cut(s) 856
BstX2I RGATCY 4 cut(s) 251, 447, 621, 1093
BstXI CCANNNNNNTGG 1 cut(s) 797
BstYI RGATCY 4 cut(s) 251, 447, 621, 1093
Bsu15I ATCGAT 1 cut(s) 902
BsuRI GGCC 1 cut(s) 375
BsuTUI ATCGAT 1 cut(s) 902
BtgI CCRYGG 1 cut(s) 355
BtgZI GCGATG 1 cut(s) 580
BtrI CACGTC 3 cut(s) 8, 334, 1074
BtsCI GGATG 1 cut(s) 983
BveI ACCTGC 1 cut(s) 655
Cac8I GCNNGC 1 cut(s) 763
CfoI GCGC 2 cut(s) 991, 1045
Cfr10I RCCGGY 5 cut(s) 730, 761, 1078, 1084, 1244
Cfr13I GGNCC 3 cut(s) 227, 839, 1037
Cfr42I CCGCGG 1 cut(s) 358
ClaI ATCGAT 1 cut(s) 902
Csp6I GTAC 4 cut(s) 551, 556, 580, 1011
CspAI ACCGGT 1 cut(s) 1244
CviAII CATG 2 cut(s) 859, 1184
CviQI GTAC 4 cut(s) 551, 556, 580, 1011
DdeI CTNAG 3 cut(s) 74, 248, 256
Eam1104I CTCTTC 4 cut(s) 149, 762, 1001, 1025
EarI CTCTTC 4 cut(s) 149, 762, 1001, 1025
EciI GGCGGA 2 cut(s) 328, 1016
Eco130I CCWWGG 1 cut(s) 842
Eco47I GGWCC 3 cut(s) 227, 839, 1037
Eco88I CYCGRG 2 cut(s) 434, 617
EcoRII CCWGG 1 cut(s) 31
EcoT14I CCWWGG 1 cut(s) 842
EcoT22I ATGCAT 1 cut(s) 531
ErhI CCWWGG 1 cut(s) 842
Esp3I CGTCTC 5 cut(s) 77, 251, 340, 354, 514
FaeI CATG 2 cut(s) 862, 1187
FalI AAGNNNNNCTT 2 cut(s) 412, 444
FatI CATG 2 cut(s) 858, 1183
FblI GTMKAC 1 cut(s) 678
Fnu4HI GCNGC 8 cut(s) 312, 342, 355, 537, 747, 987, 1302, 1305
FokI GGATG 1 cut(s) 990
Fsp4HI GCNGC 8 cut(s) 312, 342, 355, 537, 747, 987, 1302, 1305
FspBI CTAG 5 cut(s) 573, 669, 942, 992, 1308
GlaI GCGC 2 cut(s) 990, 1044
GluI GCNGC 8 cut(s) 312, 342, 355, 537, 747, 987, 1302, 1305
HaeII RGCGCY 1 cut(s) 992
HaeIII GGCC 1 cut(s) 375
HhaI GCGC 2 cut(s) 991, 1045
Hin1I GRCGYC 1 cut(s) 477
Hin1II CATG 2 cut(s) 862, 1187
Hin6I GCGC 2 cut(s) 989, 1043
HinP1I GCGC 2 cut(s) 989, 1043
HinfI GANTC 8 cut(s) 78, 264, 288, 603, 637, 684, 917, 925
HphI GGTGA 4 cut(s) 171, 820, 1111, 1178
Hpy166II GTNNAC 4 cut(s) 203, 551, 679, 1179
Hpy188III TCNNGA 5 cut(s) 11, 43, 436, 502, 619
Hpy8I GTNNAC 4 cut(s) 203, 551, 679, 1179
Hpy99I CGWCG 4 cut(s) 326, 479, 482, 1078
HpyAV CCTTC 7 cut(s) 33, 124, 183, 455, 523, 967, 1123
HpyCH4III ACNGT 7 cut(s) 162, 183, 295, 555, 832, 937, 1166
HpyCH4IV ACGT 9 cut(s) 7, 244, 333, 477, 522, 582, 1073, 1170, 1207
HpyCH4V TGCA 5 cut(s) 67, 104, 529, 627, 1240
HpyF10VI GCNNNNNNNGC 4 cut(s) 363, 372, 425, 542
HpyF3I CTNAG 3 cut(s) 74, 248, 256
HpySE526I ACGT 9 cut(s) 7, 244, 333, 477, 522, 582, 1073, 1170, 1207
Hsp92I GRCGYC 1 cut(s) 477
Hsp92II CATG 2 cut(s) 862, 1187
HspAI GCGC 2 cut(s) 989, 1043
KroI GCCGGC 1 cut(s) 761
KroNI GCCGGC 1 cut(s) 763
KspI CCGCGG 1 cut(s) 358
LmnI GCTCC 2 cut(s) 308, 677
Lsp1109I GCAGC 2 cut(s) 323, 548
LweI GCATC 2 cut(s) 76, 1143
MaeI CTAG 5 cut(s) 573, 669, 942, 992, 1308
MaeII ACGT 9 cut(s) 7, 244, 333, 477, 522, 582, 1073, 1170, 1207
MaeIII GTNAC 3 cut(s) 3, 301, 1166
MfeI CAATTG 2 cut(s) 1046, 1118
MflI RGATCY 4 cut(s) 251, 447, 621, 1093
MluCI AATT 5 cut(s) 38, 482, 1046, 1118, 1141
MlyI GAGTC 1 cut(s) 678
MmeI TCCRAC 1 cut(s) 304
Mph1103I ATGCAT 1 cut(s) 531
MroNI GCCGGC 1 cut(s) 761
MseI TTAA 5 cut(s) 168, 347, 818, 1059, 1149
MslI CAYNNNNRTG 1 cut(s) 795
MspA1I CMGCKG 2 cut(s) 314, 357
MspR9I CCNGG 1 cut(s) 33
MunI CAATTG 2 cut(s) 1046, 1118
MvaI CCWGG 1 cut(s) 33
MvnI CGCG 1 cut(s) 357
MwoI GCNNNNNNNGC 4 cut(s) 363, 372, 425, 542
NaeI GCCGGC 1 cut(s) 763
NgoMIV GCCGGC 1 cut(s) 761
NlaIII CATG 2 cut(s) 862, 1187
NlaIV GGNNCC 1 cut(s) 1083
NmuCI GTSAC 2 cut(s) 3, 1166
NsiI ATGCAT 1 cut(s) 531
NspV TTCGAA 1 cut(s) 915
PaeR7I CTCGAG 2 cut(s) 434, 617
PcsI WCGNNNNNNNCGW 4 cut(s) 330, 396, 421, 603
PdiI GCCGGC 1 cut(s) 763
PfeI GAWTC 7 cut(s) 78, 264, 288, 603, 637, 917, 925
Pfl23II CGTACG 1 cut(s) 579
PinAI ACCGGT 1 cut(s) 1244
PkrI GCNGC 8 cut(s) 313, 343, 356, 538, 748, 988, 1303, 1306
PleI GAGTC 1 cut(s) 678
PpsI GAGTC 1 cut(s) 678
PsiI TTATAA 1 cut(s) 1221
Psp6I CCWGG 1 cut(s) 31
PspGI CCWGG 1 cut(s) 31
PspLI CGTACG 1 cut(s) 579
PspN4I GGNNCC 1 cut(s) 1083
PspPI GGNCC 3 cut(s) 227, 839, 1037
PsuI RGATCY 4 cut(s) 251, 447, 621, 1093
PvuII CAGCTG 1 cut(s) 314
RsaI GTAC 4 cut(s) 552, 557, 581, 1012
RsaNI GTAC 4 cut(s) 551, 556, 580, 1011
RseI CAYNNNNRTG 1 cut(s) 795
SacII CCGCGG 1 cut(s) 358
SaqAI TTAA 5 cut(s) 168, 347, 818, 1059, 1149
SatI GCNGC 8 cut(s) 312, 342, 355, 537, 747, 987, 1302, 1305
Sau96I GGNCC 3 cut(s) 227, 839, 1037
SchI GAGTC 1 cut(s) 678
ScrFI CCNGG 1 cut(s) 33
SfaNI GCATC 2 cut(s) 76, 1143
SfcI CTRYAG 1 cut(s) 651
Sfr274I CTCGAG 2 cut(s) 434, 617
Sfr303I CCGCGG 1 cut(s) 358
SfuI TTCGAA 1 cut(s) 915
SgrAI CRCCGGYG 1 cut(s) 1078
SgrBI CCGCGG 1 cut(s) 358
SinI GGWCC 3 cut(s) 227, 839, 1037
SlaI CTCGAG 2 cut(s) 434, 617
SmiMI CAYNNNNRTG 1 cut(s) 795
SmlI CTYRAG 3 cut(s) 434, 540, 617
SmoI CTYRAG 3 cut(s) 434, 540, 617
Sse9I AATT 5 cut(s) 38, 482, 1046, 1118, 1141
SsiI CCGC 9 cut(s) 339, 342, 355, 357, 747, 986, 1001, 1302, 1305
SspI AATATT 1 cut(s) 1192
SspMI CTAG 5 cut(s) 573, 669, 942, 992, 1308
StyD4I CCNGG 1 cut(s) 31
StyI CCWWGG 1 cut(s) 842
TaaI ACNGT 7 cut(s) 162, 183, 295, 555, 832, 937, 1166
TaiI ACGT 9 cut(s) 10, 247, 336, 480, 525, 585, 1076, 1173, 1210
TaqI TCGA 8 cut(s) 262, 378, 435, 480, 618, 902, 908, 915
TasI AATT 5 cut(s) 38, 482, 1046, 1118, 1141
TatI WGTACW 1 cut(s) 1010
TauI GCSGC 6 cut(s) 344, 357, 749, 989, 1304, 1307
TfiI GAWTC 7 cut(s) 78, 264, 288, 603, 637, 917, 925
Tru1I TTAA 5 cut(s) 168, 347, 818, 1059, 1149
Tru9I TTAA 5 cut(s) 168, 347, 818, 1059, 1149
TseFI GTSAC 2 cut(s) 3, 1166
TseI GCWGC 2 cut(s) 311, 536
Tsp45I GTSAC 2 cut(s) 3, 1166
TspDTI ATGAA 2 cut(s) 442, 575
TspGWI ACGGA 9 cut(s) 237, 300, 479, 664, 690, 696, 945, 1164, 1247
VpaK11BI GGWCC 3 cut(s) 227, 839, 1037
XapI RAATTY 2 cut(s) 38, 1141
XhoI CTCGAG 2 cut(s) 434, 617
XmiI GTMKAC 1 cut(s) 678
XspI CTAG 5 cut(s) 573, 669, 942, 992, 1308
ZraI GACGTC 1 cut(s) 478
Zsp2I ATGCAT 1 cut(s) 531
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.