AT2G47840

Protein TIC 20-II, chloroplastic-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
2
OG0017208
Physical Location & Seq
Reverse (-)
19594187 .. 19595001
815 bp
Loading structure...
UTR
Exon/CDS
Intron
AT2G47840.1

Sequence Viewer

Length: 627 bp
ATGGCGTCTCTGTGCCTTTCTCTCCACCAAACCCTAACTAATCCCCTGTCGGCTCCCAGATGCAGACCACTCAGCCTCTCCTTCCCCGGATCCTCAACCTTCTCGATCCGCCCCTCTAGCAGACGCGCAACCGCCTTGACGACCCGCGCCTCCTACACCCCGACGCCGGCGACTGAGCGCGTGATTTCGATAGCGTCGTACGCGCTGCCGTTCTTCAATTCTCTGCAGTACGGGCGGTTCCTGTTCGCGCAGTACCCAAGGCTGGGATTGCTGTTCGAGCCAATCTTCCCAATCCTGAACCTGTACAGATCGGTGCCGTACGCGAGCTTCGTGGCATTCTTCGGGCTGTACCTGGGAGTGGTGAGGAACACGAGTTTCAGTAGGTACGTGAGGTTCAACGCGATGCAGGCGGTGACGCTGGATGTGCTCCTGGCGGTTCCGGTGCTGCTGACCCGAATACTGGATCCGGGTCAAGGAGGCGGGTTTGGAATGAAGGCGATGATGTGGGGGCACACGGGGGTTTTCGTCTTCAGCTTTATGTGTTTTGTGTATGGAGTCGTCAGCTCCTTACTCGGCAAAACTCCATACATTCCATTTGTCGCTGATGCCGCCGGTAGACAACTCTAA

Protein Analysis

208

Amino Acids

22.91

Weight (kDa)

10.28

Isoelectric Point (pI)

35.83

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIC20 PF16166 39 - 204 8.6e-51 Chloroplast import apparatus Tic20-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0017208)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G47840
fragaria_vesca FvH4_6g00330
malus_domestica MD12G1263500.v1.1
prunus_persica Prupe.6G362900_v2.0.a1
rosa_chinensis RchiOBHm_Chr3g0447631
rosa_laevigata RLG00000025982
rosa_multiflora Rmu_sc0003550.1_g000002
rosa_roxburghii Rroxscaffold_6G00425840
rosa_rugosa Rorug02G0603800
rosa_samantha Rh3BG003400 Rh3CG003700 Rh3DG003700
rosa_wichuraiana Rw3G000280

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 313
AccI GTMKAC 1 cut(s) 616
AccII CGCG 7 cut(s) 126, 147, 180, 203, 248, 323, 401
AciI CCGC 8 cut(s) 109, 132, 145, 235, 410, 434, 480, 609
AclWI GGATC 5 cut(s) 84, 97, 100, 458, 471
AcuI CTGAAG 1 cut(s) 514
AcyI GRCGYC 2 cut(s) 5, 164
AfaI GTAC 7 cut(s) 200, 230, 254, 305, 320, 350, 386
AfiI CCNNNNNNNGG 6 cut(s) 166, 262, 263, 358, 460, 473
AgsI TTSAA 2 cut(s) 217, 397
AjnI CCWGG 2 cut(s) 351, 429
AluBI AGCT 3 cut(s) 327, 534, 564
AluI AGCT 3 cut(s) 327, 534, 564
Alw21I GWGCWC 1 cut(s) 429
Alw26I GTCTC 1 cut(s) 12
AlwI GGATC 5 cut(s) 84, 97, 100, 458, 471
ApeKI GCWGC 2 cut(s) 205, 445
AspLEI GCGC 5 cut(s) 128, 149, 180, 205, 250
AsuC2I CCSGG 2 cut(s) 87, 468
AsuHPI GGTGA 2 cut(s) 373, 424
BaeGI GKGCMC 1 cut(s) 513
BamHI GGATCC 2 cut(s) 89, 463
BanI GGYRCC 1 cut(s) 313
BarI GAAGNNNNNNTAC 2 cut(s) 311, 343
BauI CACGAG 1 cut(s) 370
BbsI GAAGAC 1 cut(s) 520
Bbv12I GWGCWC 1 cut(s) 429
BbvI GCAGC 2 cut(s) 192, 432
BceAI ACGGC 2 cut(s) 193, 301
BciT130I CCWGG 2 cut(s) 353, 431
BcnI CCSGG 2 cut(s) 87, 468
BcoDI GTCTC 1 cut(s) 12
BfaI CTAG 1 cut(s) 117
BfmI CTRYAG 1 cut(s) 224
BisI GCNGC 3 cut(s) 206, 446, 609
BlsI GCNGC 3 cut(s) 207, 447, 610
Bme1390I CCNGG 4 cut(s) 87, 353, 431, 468
BmiI GGNNCC 6 cut(s) 54, 91, 239, 315, 438, 465
BmrFI CCNGG 4 cut(s) 87, 353, 431, 468
BmsI GCATC 3 cut(s) 50, 393, 595
BpiI GAAGAC 1 cut(s) 520
BpuMI CCSGG 2 cut(s) 87, 468
BsaAI YACGTR 1 cut(s) 388
BsaHI GRCGYC 2 cut(s) 5, 164
BsaJI CCNNGG 3 cut(s) 85, 257, 352
BsaWI WCCGGW 1 cut(s) 439
Bsc4I CCNNNNNNNGG 6 cut(s) 166, 262, 263, 358, 460, 473
Bse118I RCCGGY 2 cut(s) 166, 611
Bse1I ACTGG 1 cut(s) 465
BseBI CCWGG 2 cut(s) 353, 431
BseDI CCNNGG 3 cut(s) 85, 257, 352
BseGI GGATG 1 cut(s) 427
BseLI CCNNNNNNNGG 6 cut(s) 166, 262, 263, 358, 460, 473
BseMII CTCAG 2 cut(s) 85, 165
BseNI ACTGG 1 cut(s) 465
BseSI GKGCMC 1 cut(s) 513
BseXI GCAGC 2 cut(s) 192, 432
BseYI CCCAGC 1 cut(s) 262
Bsh1236I CGCG 7 cut(s) 126, 147, 180, 203, 248, 323, 401
BshNI GGYRCC 1 cut(s) 313
BsiHKAI GWGCWC 1 cut(s) 429
BsiSI CCGG 5 cut(s) 87, 167, 440, 467, 612
BsiWI CGTACG 2 cut(s) 198, 318
BslI CCNNNNNNNGG 6 cut(s) 166, 262, 263, 358, 460, 473
BsmAI GTCTC 1 cut(s) 12
BsmBI CGTCTC 1 cut(s) 12
BsmI GAATGC 1 cut(s) 335
Bsp1286I GDGCHC 2 cut(s) 429, 513
Bsp1407I TGTACA 1 cut(s) 303
Bsp143I GATC 4 cut(s) 89, 105, 308, 463
BspACI CCGC 8 cut(s) 109, 132, 145, 235, 410, 434, 480, 609
BspCNI CTCAG 2 cut(s) 84, 166
BspFNI CGCG 7 cut(s) 126, 147, 180, 203, 248, 323, 401
BspLI GGNNCC 6 cut(s) 54, 91, 239, 315, 438, 465
BspMAI CTGCAG 1 cut(s) 228
BspPI GGATC 5 cut(s) 84, 97, 100, 458, 471
BspT107I GGYRCC 1 cut(s) 313
BsrFI RCCGGY 2 cut(s) 166, 611
BsrGI TGTACA 1 cut(s) 303
BsrI ACTGG 1 cut(s) 465
BssAI RCCGGY 2 cut(s) 166, 611
BssECI CCNNGG 3 cut(s) 85, 257, 352
BssMI GATC 4 cut(s) 89, 105, 308, 463
BssNI GRCGYC 2 cut(s) 5, 164
BssSI CACGAG 1 cut(s) 370
BssT1I CCWWGG 1 cut(s) 257
Bst2BI CACGAG 1 cut(s) 370
Bst2UI CCWGG 2 cut(s) 353, 431
BstACI GRCGYC 2 cut(s) 5, 164
BstAUI TGTACA 1 cut(s) 303
BstBAI YACGTR 1 cut(s) 388
BstC8I GCNNGC 3 cut(s) 168, 325, 408
BstDEI CTNAG 2 cut(s) 71, 174
BstF5I GGATG 1 cut(s) 427
BstFNI CGCG 7 cut(s) 126, 147, 180, 203, 248, 323, 401
BstHHI GCGC 5 cut(s) 128, 149, 180, 205, 250
BstKTI GATC 4 cut(s) 92, 108, 311, 466
BstMAI GTCTC 1 cut(s) 12
BstMBI GATC 4 cut(s) 89, 105, 308, 463
BstMWI GCNNNNNNNGC 8 cut(s) 117, 200, 232, 268, 277, 407, 424, 608
BstNI CCWGG 2 cut(s) 353, 431
BstSCI CCNGG 4 cut(s) 85, 351, 429, 466
BstSFI CTRYAG 1 cut(s) 224
BstSLI GKGCMC 1 cut(s) 513
BstUI CGCG 7 cut(s) 126, 147, 180, 203, 248, 323, 401
BstV1I GCAGC 2 cut(s) 192, 432
BstV2I GAAGAC 1 cut(s) 520
BstX2I RGATCY 2 cut(s) 89, 463
BstYI RGATCY 2 cut(s) 89, 463
BtgZI GCGATG 2 cut(s) 416, 512
BtsCI GGATG 1 cut(s) 427
Cac8I GCNNGC 3 cut(s) 168, 325, 408
CfoI GCGC 5 cut(s) 128, 149, 180, 205, 250
Cfr10I RCCGGY 2 cut(s) 166, 611
CseI GACGC 4 cut(s) 132, 172, 183, 424
Csp6I GTAC 7 cut(s) 199, 229, 253, 304, 319, 349, 385
CviJI RGCY 8 cut(s) 53, 75, 262, 280, 327, 346, 534, 564
CviKI_1 RGCY 8 cut(s) 53, 75, 262, 280, 327, 346, 534, 564
CviQI GTAC 7 cut(s) 199, 229, 253, 304, 319, 349, 385
DdeI CTNAG 2 cut(s) 71, 174
DpnI GATC 4 cut(s) 91, 107, 310, 465
DpnII GATC 4 cut(s) 89, 105, 308, 463
EciI GGCGGA 1 cut(s) 98
Eco130I CCWWGG 1 cut(s) 257
Eco57I CTGAAG 1 cut(s) 514
EcoRII CCWGG 2 cut(s) 351, 429
EcoT14I CCWWGG 1 cut(s) 257
ErhI CCWWGG 1 cut(s) 257
Esp3I CGTCTC 1 cut(s) 12
FaiI YATR 3 cut(s) 539, 552, 586
FauI CCCGC 2 cut(s) 152, 473
FblI GTMKAC 1 cut(s) 616
Fnu4HI GCNGC 3 cut(s) 206, 446, 609
FokI GGATG 1 cut(s) 434
Fsp4HI GCNGC 3 cut(s) 206, 446, 609
FspBI CTAG 1 cut(s) 117
GlaI GCGC 5 cut(s) 127, 148, 179, 204, 249
GluI GCNGC 3 cut(s) 206, 446, 609
GsaI CCCAGC 1 cut(s) 266
HapII CCGG 5 cut(s) 87, 167, 440, 467, 612
HgaI GACGC 4 cut(s) 132, 172, 183, 424
HhaI GCGC 5 cut(s) 128, 149, 180, 205, 250
Hin1I GRCGYC 2 cut(s) 5, 164
Hin6I GCGC 5 cut(s) 126, 147, 178, 203, 248
HinP1I GCGC 5 cut(s) 126, 147, 178, 203, 248
HinfI GANTC 1 cut(s) 555
HpaII CCGG 5 cut(s) 87, 167, 440, 467, 612
HphI GGTGA 2 cut(s) 373, 424
Hpy166II GTNNAC 1 cut(s) 617
Hpy188III TCNNGA 2 cut(s) 103, 295
Hpy8I GTNNAC 1 cut(s) 617
Hpy99I CGWCG 2 cut(s) 166, 199
HpyAV CCTTC 3 cut(s) 91, 109, 487
HpyCH4IV ACGT 1 cut(s) 387
HpyCH4V TGCA 3 cut(s) 63, 226, 406
HpyF10VI GCNNNNNNNGC 8 cut(s) 117, 200, 232, 268, 277, 407, 424, 608
HpyF3I CTNAG 2 cut(s) 71, 174
HpySE526I ACGT 1 cut(s) 387
Hsp92I GRCGYC 2 cut(s) 5, 164
HspAI GCGC 5 cut(s) 126, 147, 178, 203, 248
KroI GCCGGC 1 cut(s) 166
KroNI GCCGGC 1 cut(s) 168
Kzo9I GATC 4 cut(s) 89, 105, 308, 463
LmnI GCTCC 3 cut(s) 58, 432, 569
Lsp1109I GCAGC 2 cut(s) 192, 432
LweI GCATC 3 cut(s) 50, 393, 595
MaeI CTAG 1 cut(s) 117
MaeII ACGT 1 cut(s) 387
MaeIII GTNAC 1 cut(s) 412
MalI GATC 4 cut(s) 91, 107, 310, 465
MboI GATC 4 cut(s) 89, 105, 308, 463
MboII GAAGA 4 cut(s) 205, 277, 331, 520
MflI RGATCY 2 cut(s) 89, 463
MhlI GDGCHC 2 cut(s) 429, 513
MluCI AATT 1 cut(s) 217
MlyI GAGTC 1 cut(s) 564
MnlI CCTC 7 cut(s) 86, 103, 124, 160, 357, 384, 470
MreI CGCCGGCG 1 cut(s) 166
MroNI GCCGGC 1 cut(s) 166
MspI CCGG 5 cut(s) 87, 167, 440, 467, 612
MspR9I CCNGG 4 cut(s) 87, 353, 431, 468
Mva1269I GAATGC 1 cut(s) 335
MvaI CCWGG 2 cut(s) 353, 431
MvnI CGCG 7 cut(s) 126, 147, 180, 203, 248, 323, 401
MwoI GCNNNNNNNGC 8 cut(s) 117, 200, 232, 268, 277, 407, 424, 608
NaeI GCCGGC 1 cut(s) 168
NciI CCSGG 2 cut(s) 87, 468
NdeII GATC 4 cut(s) 89, 105, 308, 463
NgoMIV GCCGGC 1 cut(s) 166
NlaIV GGNNCC 6 cut(s) 54, 91, 239, 315, 438, 465
NmeAIII GCCGAG 1 cut(s) 552
NmuCI GTSAC 1 cut(s) 412
PcsI WCGNNNNNNNCGW 2 cut(s) 194, 327
PctI GAATGC 1 cut(s) 335
PdiI GCCGGC 1 cut(s) 168
Pfl23II CGTACG 2 cut(s) 198, 318
PkrI GCNGC 3 cut(s) 207, 447, 610
PleI GAGTC 1 cut(s) 563
PpsI GAGTC 1 cut(s) 563
Ppu21I YACGTR 1 cut(s) 388
Psp6I CCWGG 2 cut(s) 351, 429
PspFI CCCAGC 1 cut(s) 262
PspGI CCWGG 2 cut(s) 351, 429
PspLI CGTACG 2 cut(s) 198, 318
PspN4I GGNNCC 6 cut(s) 54, 91, 239, 315, 438, 465
PsrI GAACNNNNNNTAC 4 cut(s) 221, 253, 377, 409
PstI CTGCAG 1 cut(s) 228
PsuI RGATCY 2 cut(s) 89, 463
RsaI GTAC 7 cut(s) 200, 230, 254, 305, 320, 350, 386
RsaNI GTAC 7 cut(s) 199, 229, 253, 304, 319, 349, 385
SatI GCNGC 3 cut(s) 206, 446, 609
Sau3AI GATC 4 cut(s) 89, 105, 308, 463
SchI GAGTC 1 cut(s) 564
ScrFI CCNGG 4 cut(s) 87, 353, 431, 468
SduI GDGCHC 2 cut(s) 429, 513
SetI ASST 9 cut(s) 101, 303, 329, 354, 386, 390, 395, 536, 566
SfaNI GCATC 3 cut(s) 50, 393, 595
SfcI CTRYAG 1 cut(s) 224
SgrAI CRCCGGYG 1 cut(s) 166
Sse9I AATT 1 cut(s) 217
SsiI CCGC 8 cut(s) 109, 132, 145, 235, 410, 434, 480, 609
SspMI CTAG 1 cut(s) 117
StyD4I CCNGG 4 cut(s) 85, 351, 429, 466
StyI CCWWGG 1 cut(s) 257
TaiI ACGT 1 cut(s) 390
TaqI TCGA 3 cut(s) 104, 188, 276
TasI AATT 1 cut(s) 217
TatI WGTACW 1 cut(s) 303
TauI GCSGC 1 cut(s) 611
TseFI GTSAC 1 cut(s) 412
TseI GCWGC 2 cut(s) 205, 445
Tsp45I GTSAC 1 cut(s) 412
TspDTI ATGAA 1 cut(s) 506
XmiI GTMKAC 1 cut(s) 616
XspI CTAG 1 cut(s) 117
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.