Rorug02G0603800

tic 20-ii

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0017208
Physical Location & Seq
Forward (+)
72201154 .. 72201665
512 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0603800.1

Sequence Viewer

Length: 414 bp
ATGTTTCTCGAAATTCCAGTCATCTCCGATGAGGATCCGAGCCGCTCTTCAGTGGAGAAGAAGGCCCGATCTGGAGAGATCGATGTGGAAGACCCAGATTCAAACTACTACTCAGATGGATCAGAAGAAGAAATTAAACATTGTGAAGTTTGTATGAAGGACGAGGAACACTGGTCATATGATTGCCCGTACTTGGACTATATTGAGTATATTCCAAACCCGGAGGACACACCTGTTGGCGAGGGCTATATCATAATTTGCATCCACTGCAGTGAGTCGAAACATGGCCACCCTGATGGAAGCTGGGTAGGACGTGCAACAAGAAATCCTTTTGATTATAGTTTCATATTTAAAAAGCCTGGTGTGAATATGATGATGACGATGTATTTGATTAATGTGGGTGCTCGTTTGTAA

Protein Analysis

137

Amino Acids

15.74

Weight (kDa)

4.51

Isoelectric Point (pI)

48.81

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0017208)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G47840
fragaria_vesca FvH4_6g00330
malus_domestica MD12G1263500.v1.1
prunus_persica Prupe.6G362900_v2.0.a1
rosa_chinensis RchiOBHm_Chr3g0447631
rosa_laevigata RLG00000025982
rosa_multiflora Rmu_sc0003550.1_g000002
rosa_roxburghii Rroxscaffold_6G00425840
rosa_rugosa Rorug02G0603800
rosa_samantha Rh3BG003400 Rh3CG003700 Rh3DG003700
rosa_wichuraiana Rw3G000280

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 45
AciI CCGC 1 cut(s) 43
AclWI GGATC 3 cut(s) 29, 42, 127
AcoI YGGCCR 1 cut(s) 286
AcsI RAATTY 1 cut(s) 12
AcuI CTGAAG 1 cut(s) 33
AfaI GTAC 1 cut(s) 191
AfiI CCNNNNNNNGG 1 cut(s) 193
AgsI TTSAA 1 cut(s) 102
AjiI CACGTC 1 cut(s) 314
AjnI CCWGG 1 cut(s) 358
AleI CACNNNNGTG 1 cut(s) 270
AluBI AGCT 1 cut(s) 303
AluI AGCT 1 cut(s) 303
Alw21I GWGCWC 1 cut(s) 406
AlwI GGATC 3 cut(s) 29, 42, 127
AoxI GGCC 2 cut(s) 63, 286
ApoI RAATTY 1 cut(s) 12
ArsI GACNNNNNNTTYG 3 cut(s) 35, 370, 402
AseI ATTAAT 1 cut(s) 393
AspS9I GGNCC 1 cut(s) 64
AsuC2I CCSGG 1 cut(s) 221
BalI TGGCCA 1 cut(s) 288
BamHI GGATCC 1 cut(s) 34
BbsI GAAGAC 1 cut(s) 96
Bbv12I GWGCWC 1 cut(s) 406
BccI CCATC 2 cut(s) 110, 290
BciT130I CCWGG 1 cut(s) 360
BcnI CCSGG 1 cut(s) 221
BfmI CTRYAG 1 cut(s) 268
BisI GCNGC 1 cut(s) 43
BlsI GCNGC 1 cut(s) 44
Bme1390I CCNGG 2 cut(s) 221, 360
BmgBI CACGTC 1 cut(s) 314
BmgT120I GGNCC 1 cut(s) 64
BmiI GGNNCC 1 cut(s) 36
BmrFI CCNGG 2 cut(s) 221, 360
BmsI GCATC 1 cut(s) 270
BpiI GAAGAC 1 cut(s) 96
BpmI CTGGAG 1 cut(s) 93
BpuMI CCSGG 1 cut(s) 221
Bsa29I ATCGAT 1 cut(s) 81
BsaBI GATNNNNATC 1 cut(s) 33
Bsc4I CCNNNNNNNGG 1 cut(s) 193
Bse1I ACTGG 2 cut(s) 17, 176
Bse8I GATNNNNATC 1 cut(s) 33
BseBI CCWGG 1 cut(s) 360
BseCI ATCGAT 1 cut(s) 81
BseGI GGATG 1 cut(s) 261
BseJI GATNNNNATC 1 cut(s) 33
BseLI CCNNNNNNNGG 1 cut(s) 193
BseMII CTCAG 1 cut(s) 126
BseNI ACTGG 2 cut(s) 17, 176
BseYI CCCAGC 1 cut(s) 303
BshFI GGCC 2 cut(s) 65, 288
BshVI ATCGAT 1 cut(s) 81
BsiHKAI GWGCWC 1 cut(s) 406
BsiSI CCGG 1 cut(s) 221
BslI CCNNNNNNNGG 1 cut(s) 193
BsnI GGCC 2 cut(s) 65, 288
Bsp1286I GDGCHC 1 cut(s) 406
Bsp143I GATC 4 cut(s) 34, 68, 78, 119
BspACI CCGC 1 cut(s) 43
BspANI GGCC 2 cut(s) 65, 288
BspCNI CTCAG 1 cut(s) 125
BspDI ATCGAT 1 cut(s) 81
BspLI GGNNCC 1 cut(s) 36
BspMAI CTGCAG 1 cut(s) 272
BspPI GGATC 3 cut(s) 29, 42, 127
BspQI GCTCTTC 1 cut(s) 52
BsrBI CCGCTC 1 cut(s) 45
BsrI ACTGG 2 cut(s) 17, 176
BssMI GATC 4 cut(s) 34, 68, 78, 119
Bst2UI CCWGG 1 cut(s) 360
Bst6I CTCTTC 1 cut(s) 52
BstAPI GCANNNNNTGC 1 cut(s) 267
BstDEI CTNAG 1 cut(s) 112
BstF5I GGATG 1 cut(s) 261
BstKTI GATC 4 cut(s) 37, 71, 81, 122
BstMBI GATC 4 cut(s) 34, 68, 78, 119
BstMWI GCNNNNNNNGC 1 cut(s) 267
BstNI CCWGG 1 cut(s) 360
BstSCI CCNGG 2 cut(s) 219, 358
BstSFI CTRYAG 1 cut(s) 268
BstV2I GAAGAC 1 cut(s) 96
BstX2I RGATCY 1 cut(s) 34
BstXI CCANNNNNNTGG 1 cut(s) 296
BstYI RGATCY 1 cut(s) 34
Bsu15I ATCGAT 1 cut(s) 81
BsuRI GGCC 2 cut(s) 65, 288
BsuTUI ATCGAT 1 cut(s) 81
BtrI CACGTC 1 cut(s) 314
BtsCI GGATG 1 cut(s) 261
BtsI GCAGTG 2 cut(s) 265, 277
BtsIMutI CAGTG 4 cut(s) 57, 169, 265, 277
Cfr13I GGNCC 1 cut(s) 64
ClaI ATCGAT 1 cut(s) 81
Csp6I GTAC 1 cut(s) 190
CviAII CATG 1 cut(s) 284
CviJI RGCY 6 cut(s) 42, 65, 246, 288, 303, 358
CviKI_1 RGCY 6 cut(s) 42, 65, 246, 288, 303, 358
CviQI GTAC 1 cut(s) 190
DdeI CTNAG 1 cut(s) 112
DpnI GATC 4 cut(s) 36, 70, 80, 121
DpnII GATC 4 cut(s) 34, 68, 78, 119
DraI TTTAAA 1 cut(s) 352
EaeI YGGCCR 1 cut(s) 286
Eam1104I CTCTTC 1 cut(s) 52
EarI CTCTTC 1 cut(s) 52
Eco57I CTGAAG 1 cut(s) 33
EcoRII CCWGG 1 cut(s) 358
FaeI CATG 1 cut(s) 287
FalI AAGNNNNNCTT 2 cut(s) 313, 345
FatI CATG 1 cut(s) 283
FauNDI CATATG 1 cut(s) 178
Fnu4HI GCNGC 1 cut(s) 43
FokI GGATG 1 cut(s) 248
Fsp4HI GCNGC 1 cut(s) 43
GluI GCNGC 1 cut(s) 43
GsaI CCCAGC 1 cut(s) 307
GsuI CTGGAG 1 cut(s) 93
HaeIII GGCC 2 cut(s) 65, 288
HapII CCGG 1 cut(s) 221
Hin1II CATG 1 cut(s) 287
HinfI GANTC 2 cut(s) 98, 275
HpaII CCGG 1 cut(s) 221
Hpy188I TCNGA 4 cut(s) 28, 39, 115, 124
Hpy188III TCNNGA 2 cut(s) 8, 72
HpyAV CCTTC 2 cut(s) 55, 151
HpyCH4IV ACGT 1 cut(s) 313
HpyCH4V TGCA 3 cut(s) 261, 270, 317
HpyF10VI GCNNNNNNNGC 1 cut(s) 267
HpyF3I CTNAG 1 cut(s) 112
HpySE526I ACGT 1 cut(s) 313
Hsp92II CATG 1 cut(s) 287
Kzo9I GATC 4 cut(s) 34, 68, 78, 119
LguI GCTCTTC 1 cut(s) 52
LweI GCATC 1 cut(s) 270
MaeII ACGT 1 cut(s) 313
MalI GATC 4 cut(s) 36, 70, 80, 121
MbiI CCGCTC 1 cut(s) 45
MboI GATC 4 cut(s) 34, 68, 78, 119
MboII GAAGA 5 cut(s) 39, 70, 101, 137, 140
MflI RGATCY 1 cut(s) 34
MhlI GDGCHC 1 cut(s) 406
MlsI TGGCCA 1 cut(s) 288
MluCI AATT 3 cut(s) 12, 132, 255
MluNI TGGCCA 1 cut(s) 288
MlyI GAGTC 1 cut(s) 284
MnlI CCTC 4 cut(s) 25, 157, 217, 235
Mox20I TGGCCA 1 cut(s) 288
MscI TGGCCA 1 cut(s) 288
MseI TTAA 3 cut(s) 135, 351, 393
MslI CAYNNNNRTG 2 cut(s) 270, 294
Msp20I TGGCCA 1 cut(s) 288
MspI CCGG 1 cut(s) 221
MspR9I CCNGG 2 cut(s) 221, 360
MvaI CCWGG 1 cut(s) 360
MwoI GCNNNNNNNGC 1 cut(s) 267
NciI CCSGG 1 cut(s) 221
NdeI CATATG 1 cut(s) 178
NdeII GATC 4 cut(s) 34, 68, 78, 119
NlaIII CATG 1 cut(s) 287
NlaIV GGNNCC 1 cut(s) 36
OliI CACNNNNGTG 1 cut(s) 270
PciSI GCTCTTC 1 cut(s) 52
PfeI GAWTC 1 cut(s) 98
PkrI GCNGC 1 cut(s) 44
PleI GAGTC 1 cut(s) 283
PpsI GAGTC 1 cut(s) 283
PshBI ATTAAT 1 cut(s) 393
Psp6I CCWGG 1 cut(s) 358
PspFI CCCAGC 1 cut(s) 303
PspGI CCWGG 1 cut(s) 358
PspN4I GGNNCC 1 cut(s) 36
PspPI GGNCC 1 cut(s) 64
PstI CTGCAG 1 cut(s) 272
PsuI RGATCY 1 cut(s) 34
RsaI GTAC 1 cut(s) 191
RsaNI GTAC 1 cut(s) 190
RseI CAYNNNNRTG 2 cut(s) 270, 294
SapI GCTCTTC 1 cut(s) 52
SaqAI TTAA 3 cut(s) 135, 351, 393
SatI GCNGC 1 cut(s) 43
Sau3AI GATC 4 cut(s) 34, 68, 78, 119
Sau96I GGNCC 1 cut(s) 64
SchI GAGTC 1 cut(s) 284
ScrFI CCNGG 2 cut(s) 221, 360
SduI GDGCHC 1 cut(s) 406
SetI ASST 3 cut(s) 235, 305, 316
SfaNI GCATC 1 cut(s) 270
SfcI CTRYAG 1 cut(s) 268
SmiMI CAYNNNNRTG 2 cut(s) 270, 294
Sse9I AATT 3 cut(s) 12, 132, 255
SsiI CCGC 1 cut(s) 43
StyD4I CCNGG 2 cut(s) 219, 358
TaiI ACGT 1 cut(s) 316
TaqI TCGA 3 cut(s) 9, 81, 278
TasI AATT 3 cut(s) 12, 132, 255
TauI GCSGC 1 cut(s) 45
TfiI GAWTC 1 cut(s) 98
Tru1I TTAA 3 cut(s) 135, 351, 393
Tru9I TTAA 3 cut(s) 135, 351, 393
TscAI CASTG 4 cut(s) 57, 176, 272, 277
TspDTI ATGAA 2 cut(s) 170, 334
TspRI CASTG 4 cut(s) 57, 176, 272, 277
VspI ATTAAT 1 cut(s) 393
XapI RAATTY 1 cut(s) 12
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.