MD12G1263500.v1.1

tic 20-ii

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0017208
Physical Location & Seq
Reverse (-)
32995101 .. 32995739
639 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1263500.v1.1.491

Sequence Viewer

Length: 639 bp
ATGGCCTCTCTCCTCCACCTCTCCGCCACCCCCAAAACCCTAATCCCCAAATCTTACATTCCCTCCTCCGCGTCGCGCGCTTTCCCCTCAACCCTCTCCACCCGGTCACTCTCCTTCCCTCAACTTGTTGGCCGAATATCCAAACCCAAATCCTCCACCATTACCTGCAGCACACCGACCCCCGCCACGGATCGATTGATCTCCGCTGTCGCTTACACACTCCCTTTCTTCAACTCCCTCCAGTATGGACGCTACCTGTTTATCCAATTCCCCAAATTGGGCCTCCTCTTCGAACCCTTGATCCCCTTGCTGAGCCTCTATCGGTCGATGCCGTACTCGAGCTTCGTGGCCTTCTTTGCCCTCTACCTGGGCGTCGTCAGAAACCCAAGCTTCAACCACTACGTGCGGTTCAACGCGATGCAGGCGGTGACGCTGGACGTCCTGTTGGTACTGCCGCTGCTGGTCCAGAGGATTTTCAGTCCGGGTCGGGCCGGGTTGGGGTACAAGCTCATGGTTTGGGGGCATAATGGTATTTTCGTCTTCAGCGTTTTCTGCTTCGTTTATGGTTTGGTTAACTGCATTTTGGGTCGGACTCCGTACCTGCCCTTTGTTGCTGATGCCGCCGGCCGGCAGCTCTGA

Protein Analysis

213

Amino Acids

23.52

Weight (kDa)

10.16

Isoelectric Point (pI)

33.14

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIC20 PF16166 65 - 208 1e-45 Chloroplast import apparatus Tic20-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017208)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G47840
fragaria_vesca FvH4_6g00330
malus_domestica MD12G1263500.v1.1
prunus_persica Prupe.6G362900_v2.0.a1
rosa_chinensis RchiOBHm_Chr3g0447631
rosa_laevigata RLG00000025982
rosa_multiflora Rmu_sc0003550.1_g000002
rosa_roxburghii Rroxscaffold_6G00425840
rosa_rugosa Rorug02G0603800
rosa_samantha Rh3BG003400 Rh3CG003700 Rh3DG003700
rosa_wichuraiana Rw3G000280

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 441
Acc36I ACCTGC 2 cut(s) 173, 609
AccII CGCG 4 cut(s) 71, 76, 78, 416
AciI CCGC 8 cut(s) 24, 69, 183, 204, 406, 425, 455, 621
AclWI GGATC 2 cut(s) 198, 295
AcoI YGGCCR 2 cut(s) 130, 625
AcuI CTGAAG 1 cut(s) 526
AcyI GRCGYC 2 cut(s) 372, 438
AdeI CACNNNGTG 1 cut(s) 403
AfaI GTAC 4 cut(s) 335, 450, 503, 599
AfiI CCNNNNNNNGG 6 cut(s) 187, 277, 278, 367, 498, 627
AgsI TTSAA 3 cut(s) 232, 394, 412
AjnI CCWGG 1 cut(s) 366
AloI GAACNNNNNNTCC 2 cut(s) 285, 317
AluBI AGCT 4 cut(s) 342, 390, 508, 634
AluI AGCT 4 cut(s) 342, 390, 508, 634
AlwI GGATC 2 cut(s) 198, 295
Ama87I CYCGRG 1 cut(s) 337
AoxI GGCC 6 cut(s) 3, 130, 280, 348, 489, 625
ApeKI GCWGC 3 cut(s) 168, 457, 631
AspLEI GCGC 2 cut(s) 78, 80
AspS9I GGNCC 3 cut(s) 280, 463, 489
AsuC2I CCSGG 3 cut(s) 103, 483, 493
AsuHPI GGTGA 1 cut(s) 439
AsuII TTCGAA 1 cut(s) 291
AvaI CYCGRG 1 cut(s) 337
AvaII GGWCC 1 cut(s) 463
BarI GAAGNNNNNNTAC 2 cut(s) 326, 358
BbsI GAAGAC 1 cut(s) 532
BbvI GCAGC 2 cut(s) 180, 444
BceAI ACGGC 1 cut(s) 316
BciT130I CCWGG 1 cut(s) 368
BcnI CCSGG 3 cut(s) 103, 483, 493
BfmI CTRYAG 1 cut(s) 166
BfuAI ACCTGC 2 cut(s) 173, 609
BisI GCNGC 5 cut(s) 169, 455, 458, 621, 632
BlpI GCTNAGC 1 cut(s) 311
BlsI GCNGC 5 cut(s) 170, 456, 459, 622, 633
Bme1390I CCNGG 4 cut(s) 103, 368, 483, 493
Bme18I GGWCC 1 cut(s) 463
BmeT110I CYCGRG 1 cut(s) 337
BmgT120I GGNCC 3 cut(s) 280, 463, 489
BmrFI CCNGG 4 cut(s) 103, 368, 483, 493
BmsI GCATC 3 cut(s) 318, 408, 607
BpiI GAAGAC 1 cut(s) 532
BpmI CTGGAG 1 cut(s) 224
Bpu1102I GCTNAGC 1 cut(s) 311
Bpu14I TTCGAA 1 cut(s) 291
BpuMI CCSGG 3 cut(s) 103, 483, 493
Bsa29I ATCGAT 1 cut(s) 193
BsaAI YACGTR 1 cut(s) 403
BsaHI GRCGYC 2 cut(s) 372, 438
BsaJI CCNNGG 2 cut(s) 186, 367
BsaXI ACNNNNNCTCC 2 cut(s) 47, 77
Bsc4I CCNNNNNNNGG 6 cut(s) 187, 277, 278, 367, 498, 627
Bse118I RCCGGY 2 cut(s) 623, 627
Bse1I ACTGG 1 cut(s) 241
BseBI CCWGG 1 cut(s) 368
BseCI ATCGAT 1 cut(s) 193
BseDI CCNNGG 2 cut(s) 186, 367
BseLI CCNNNNNNNGG 6 cut(s) 187, 277, 278, 367, 498, 627
BseMII CTCAG 1 cut(s) 302
BseNI ACTGG 1 cut(s) 241
BsePI GCGCGC 1 cut(s) 76
BseRI GAGGAG 2 cut(s) 55, 275
BseX3I CGGCCG 1 cut(s) 625
BseXI GCAGC 2 cut(s) 180, 444
Bsh1236I CGCG 4 cut(s) 71, 76, 78, 416
Bsh1285I CGRYCG 2 cut(s) 326, 628
BshFI GGCC 6 cut(s) 5, 132, 282, 350, 491, 627
BshVI ATCGAT 1 cut(s) 193
BsiEI CGRYCG 2 cut(s) 326, 628
BsiHKCI CYCGRG 1 cut(s) 337
BsiSI CCGG 5 cut(s) 103, 482, 492, 624, 628
BslI CCNNNNNNNGG 6 cut(s) 187, 277, 278, 367, 498, 627
BsnI GGCC 6 cut(s) 5, 132, 282, 350, 491, 627
BsoBI CYCGRG 1 cut(s) 337
Bsp119I TTCGAA 1 cut(s) 291
Bsp143I GATC 3 cut(s) 190, 198, 300
Bsp1720I GCTNAGC 1 cut(s) 311
BspACI CCGC 8 cut(s) 24, 69, 183, 204, 406, 425, 455, 621
BspANI GGCC 6 cut(s) 5, 132, 282, 350, 491, 627
BspCNI CTCAG 1 cut(s) 303
BspDI ATCGAT 1 cut(s) 193
BspFNI CGCG 4 cut(s) 71, 76, 78, 416
BspMAI CTGCAG 1 cut(s) 170
BspMI ACCTGC 2 cut(s) 173, 609
BspPI GGATC 2 cut(s) 198, 295
BspT104I TTCGAA 1 cut(s) 291
BsrFI RCCGGY 2 cut(s) 623, 627
BsrI ACTGG 1 cut(s) 241
BssAI RCCGGY 2 cut(s) 623, 627
BssECI CCNNGG 2 cut(s) 186, 367
BssHII GCGCGC 1 cut(s) 76
BssMI GATC 3 cut(s) 190, 198, 300
BssNI GRCGYC 2 cut(s) 372, 438
Bst2UI CCWGG 1 cut(s) 368
Bst6I CTCTTC 1 cut(s) 293
BstACI GRCGYC 2 cut(s) 372, 438
BstBAI YACGTR 1 cut(s) 403
BstBI TTCGAA 1 cut(s) 291
BstC8I GCNNGC 4 cut(s) 78, 423, 625, 629
BstDEI CTNAG 1 cut(s) 311
BstDSI CCRYGG 1 cut(s) 186
BstFNI CGCG 4 cut(s) 71, 76, 78, 416
BstHHI GCGC 2 cut(s) 78, 80
BstKTI GATC 3 cut(s) 193, 201, 303
BstMBI GATC 3 cut(s) 190, 198, 300
BstMCI CGRYCG 2 cut(s) 326, 628
BstMWI GCNNNNNNNGC 5 cut(s) 77, 356, 422, 552, 620
BstNI CCWGG 1 cut(s) 368
BstSCI CCNGG 4 cut(s) 101, 366, 481, 491
BstSFI CTRYAG 1 cut(s) 166
BstUI CGCG 4 cut(s) 71, 76, 78, 416
BstV1I GCAGC 2 cut(s) 180, 444
BstV2I GAAGAC 1 cut(s) 532
BstZI CGGCCG 1 cut(s) 625
Bsu15I ATCGAT 1 cut(s) 193
BsuRI GGCC 6 cut(s) 5, 132, 282, 350, 491, 627
BsuTUI ATCGAT 1 cut(s) 193
BtgI CCRYGG 1 cut(s) 186
BtgZI GCGATG 1 cut(s) 431
BveI ACCTGC 2 cut(s) 173, 609
Cac8I GCNNGC 4 cut(s) 78, 423, 625, 629
CfoI GCGC 2 cut(s) 78, 80
Cfr10I RCCGGY 2 cut(s) 623, 627
Cfr13I GGNCC 3 cut(s) 280, 463, 489
ClaI ATCGAT 1 cut(s) 193
CseI GACGC 4 cut(s) 60, 258, 361, 439
Csp6I GTAC 4 cut(s) 334, 449, 502, 598
CviAII CATG 1 cut(s) 511
CviQI GTAC 4 cut(s) 334, 449, 502, 598
DdeI CTNAG 1 cut(s) 311
DpnI GATC 3 cut(s) 192, 200, 302
DpnII GATC 3 cut(s) 190, 198, 300
DraIII CACNNNGTG 1 cut(s) 403
EaeI YGGCCR 2 cut(s) 130, 625
EagI CGGCCG 1 cut(s) 625
Eam1104I CTCTTC 1 cut(s) 293
EarI CTCTTC 1 cut(s) 293
EciI GGCGGA 1 cut(s) 13
EclXI CGGCCG 1 cut(s) 625
Eco47I GGWCC 1 cut(s) 463
Eco52I CGGCCG 1 cut(s) 625
Eco57I CTGAAG 1 cut(s) 526
Eco88I CYCGRG 1 cut(s) 337
EcoRII CCWGG 1 cut(s) 366
FaeI CATG 1 cut(s) 514
FaiI YATR 4 cut(s) 246, 512, 525, 564
FatI CATG 1 cut(s) 510
FauI CCCGC 1 cut(s) 190
Fnu4HI GCNGC 5 cut(s) 169, 455, 458, 621, 632
Fsp4HI GCNGC 5 cut(s) 169, 455, 458, 621, 632
GlaI GCGC 2 cut(s) 77, 79
GluI GCNGC 5 cut(s) 169, 455, 458, 621, 632
GsuI CTGGAG 1 cut(s) 224
HaeIII GGCC 6 cut(s) 5, 132, 282, 350, 491, 627
HapII CCGG 5 cut(s) 103, 482, 492, 624, 628
HgaI GACGC 4 cut(s) 60, 258, 361, 439
HhaI GCGC 2 cut(s) 78, 80
Hin1I GRCGYC 2 cut(s) 372, 438
Hin1II CATG 1 cut(s) 514
Hin6I GCGC 2 cut(s) 76, 78
HinP1I GCGC 2 cut(s) 76, 78
HincII GTYRAC 1 cut(s) 574
HindII GTYRAC 1 cut(s) 574
HindIII AAGCTT 1 cut(s) 388
HinfI GANTC 1 cut(s) 592
HpaI GTTAAC 1 cut(s) 574
HpaII CCGG 5 cut(s) 103, 482, 492, 624, 628
HphI GGTGA 1 cut(s) 439
Hpy166II GTNNAC 1 cut(s) 574
Hpy188I TCNGA 3 cut(s) 380, 591, 638
Hpy188III TCNNGA 1 cut(s) 466
Hpy8I GTNNAC 1 cut(s) 574
Hpy99I CGWCG 2 cut(s) 76, 377
HpyAV CCTTC 2 cut(s) 124, 361
HpyCH4IV ACGT 2 cut(s) 402, 438
HpyCH4V TGCA 3 cut(s) 168, 421, 579
HpyF10VI GCNNNNNNNGC 5 cut(s) 77, 356, 422, 552, 620
HpyF3I CTNAG 1 cut(s) 311
HpySE526I ACGT 2 cut(s) 402, 438
Hsp92I GRCGYC 2 cut(s) 372, 438
Hsp92II CATG 1 cut(s) 514
HspAI GCGC 2 cut(s) 76, 78
KroI GCCGGC 2 cut(s) 623, 627
KroNI GCCGGC 2 cut(s) 625, 629
KspAI GTTAAC 1 cut(s) 574
Kzo9I GATC 3 cut(s) 190, 198, 300
Lsp1109I GCAGC 2 cut(s) 180, 444
LweI GCATC 3 cut(s) 318, 408, 607
MaeII ACGT 2 cut(s) 402, 438
MaeIII GTNAC 2 cut(s) 105, 427
MalI GATC 3 cut(s) 192, 200, 302
MboI GATC 3 cut(s) 190, 198, 300
MboII GAAGA 3 cut(s) 220, 280, 532
MluCI AATT 2 cut(s) 266, 275
MlyI GAGTC 1 cut(s) 586
MmeI TCCRAC 1 cut(s) 569
MroNI GCCGGC 2 cut(s) 623, 627
MseI TTAA 1 cut(s) 573
MspA1I CMGCKG 2 cut(s) 206, 457
MspI CCGG 5 cut(s) 103, 482, 492, 624, 628
MspR9I CCNGG 4 cut(s) 103, 368, 483, 493
MvaI CCWGG 1 cut(s) 368
MvnI CGCG 4 cut(s) 71, 76, 78, 416
MwoI GCNNNNNNNGC 5 cut(s) 77, 356, 422, 552, 620
NaeI GCCGGC 2 cut(s) 625, 629
NciI CCSGG 3 cut(s) 103, 483, 493
NdeII GATC 3 cut(s) 190, 198, 300
NgoMIV GCCGGC 2 cut(s) 623, 627
NlaIII CATG 1 cut(s) 514
NmuCI GTSAC 2 cut(s) 105, 427
NspV TTCGAA 1 cut(s) 291
PaeR7I CTCGAG 1 cut(s) 337
PauI GCGCGC 1 cut(s) 76
PcsI WCGNNNNNNNCGW 1 cut(s) 543
PdiI GCCGGC 2 cut(s) 625, 629
PkrI GCNGC 5 cut(s) 170, 456, 459, 622, 633
PleI GAGTC 1 cut(s) 586
PpsI GAGTC 1 cut(s) 586
Ppu21I YACGTR 1 cut(s) 403
Psp6I CCWGG 1 cut(s) 366
PspGI CCWGG 1 cut(s) 366
PspPI GGNCC 3 cut(s) 280, 463, 489
PspXI VCTCGAGB 1 cut(s) 337
PstI CTGCAG 1 cut(s) 170
PteI GCGCGC 1 cut(s) 76
RsaI GTAC 4 cut(s) 335, 450, 503, 599
RsaNI GTAC 4 cut(s) 334, 449, 502, 598
SaqAI TTAA 1 cut(s) 573
SatI GCNGC 5 cut(s) 169, 455, 458, 621, 632
Sau3AI GATC 3 cut(s) 190, 198, 300
Sau96I GGNCC 3 cut(s) 280, 463, 489
SchI GAGTC 1 cut(s) 586
ScrFI CCNGG 4 cut(s) 103, 368, 483, 493
SfaNI GCATC 3 cut(s) 318, 408, 607
SfcI CTRYAG 1 cut(s) 166
Sfr274I CTCGAG 1 cut(s) 337
SfuI TTCGAA 1 cut(s) 291
SinI GGWCC 1 cut(s) 463
SlaI CTCGAG 1 cut(s) 337
SmlI CTYRAG 1 cut(s) 337
SmoI CTYRAG 1 cut(s) 337
Sse9I AATT 2 cut(s) 266, 275
SsiI CCGC 8 cut(s) 24, 69, 183, 204, 406, 425, 455, 621
StyD4I CCNGG 4 cut(s) 101, 366, 481, 491
TaiI ACGT 2 cut(s) 405, 441
TaqI TCGA 4 cut(s) 193, 291, 326, 338
TaqII GACCGA 1 cut(s) 312
TasI AATT 2 cut(s) 266, 275
TauI GCSGC 2 cut(s) 457, 623
Tru1I TTAA 1 cut(s) 573
Tru9I TTAA 1 cut(s) 573
TseFI GTSAC 2 cut(s) 105, 427
TseI GCWGC 3 cut(s) 168, 457, 631
Tsp45I GTSAC 2 cut(s) 105, 427
TspGWI ACGGA 2 cut(s) 203, 585
VpaK11BI GGWCC 1 cut(s) 463
XhoI CTCGAG 1 cut(s) 337
ZraI GACGTC 1 cut(s) 439
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.