FvH4_6g00330

tic 20-ii

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0017208
Physical Location & Seq
Reverse (-)
249526 .. 250140
615 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g00330.t1

Sequence Viewer

Length: 615 bp
ATGGCCTCCATCCATCTCCTCCACGGCCTCTCGCCGACTACTCCCAAAGCCCTAGCCTTACCCAGATTCCGGACCTCCACTATAGCCACCCGGTCGCTCTCTTTCCCCAAACCCAAATCGAAGACCATCACTTGCATGTCCTCCGGCACTCCCACGCCGGCCACAGATCGCTTAATCTCCGCCGTCTCATACACTCTCCCCTTCTTCAATTCCCTCCAGTACGGTCGCTACCTGTTTCTCCAGTTCCCCAAACTGGGCCTTCTCTTCGAGCCGCTGCTTCCCTTGTTGGGTCTCTACAGGTCCATCCCCTACTCGAGCTTCGTCGCCTTCTTCGCCCTCTATTTGGGTGTGGTCAGGAACCCTAGCTTCAGCCACTACGCCAGGTTCAATGCTATGCAAGCAGTTACTTTGGACGTCCTCTTGGTTCTGCCACTCCTCGTTCAGAGGATCTTCAGCCCGGGTCAGGCCGGTCTAGGCTTCAAGCTCATGGTCTGGGGCCATAATGGTATTTTCGTCTTCAGTGTTTTCTGCTTTGTTTATGGCTTGGTTAGTTCTGTTTTGGGTCGCACTCCTTACTTGCCCTTTGTTGCTGATGCCGCCGGCCGCCAGCTCTGA

Protein Analysis

205

Amino Acids

22.52

Weight (kDa)

10.29

Isoelectric Point (pI)

36.61

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIC20 PF16166 57 - 200 8.1e-46 Chloroplast import apparatus Tic20-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0017208)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G47840
fragaria_vesca FvH4_6g00330
malus_domestica MD12G1263500.v1.1
prunus_persica Prupe.6G362900_v2.0.a1
rosa_chinensis RchiOBHm_Chr3g0447631
rosa_laevigata RLG00000025982
rosa_multiflora Rmu_sc0003550.1_g000002
rosa_roxburghii Rroxscaffold_6G00425840
rosa_rugosa Rorug02G0603800
rosa_samantha Rh3BG003400 Rh3CG003700 Rh3DG003700
rosa_wichuraiana Rw3G000280

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 417
AccIII TCCGGA 1 cut(s) 69
AciI CCGC 4 cut(s) 180, 272, 597, 604
AclWI GGATC 1 cut(s) 455
AcoI YGGCCR 2 cut(s) 159, 601
AcuI CTGAAG 3 cut(s) 352, 436, 502
AcyI GRCGYC 1 cut(s) 414
AfaI GTAC 1 cut(s) 221
AfiI CCNNNNNNNGG 6 cut(s) 69, 253, 254, 287, 343, 463
AgsI TTSAA 3 cut(s) 208, 388, 481
AjnI CCWGG 1 cut(s) 380
AluBI AGCT 4 cut(s) 318, 366, 484, 610
AluI AGCT 4 cut(s) 318, 366, 484, 610
Alw26I GTCTC 2 cut(s) 190, 296
AlwI GGATC 1 cut(s) 455
Ama87I CYCGRG 2 cut(s) 313, 457
Aor13HI TCCGGA 1 cut(s) 69
AoxI GGCC 7 cut(s) 3, 25, 159, 256, 465, 496, 601
ApeKI GCWGC 1 cut(s) 274
AspS9I GGNCC 4 cut(s) 72, 256, 300, 496
AsuC2I CCSGG 3 cut(s) 91, 458, 459
AvaI CYCGRG 2 cut(s) 313, 457
AvaII GGWCC 2 cut(s) 72, 300
BbsI GAAGAC 2 cut(s) 128, 508
BbvI GCAGC 1 cut(s) 261
BccI CCATC 4 cut(s) 17, 21, 134, 311
BceAI ACGGC 2 cut(s) 40, 167
BciT130I CCWGG 1 cut(s) 382
BcnI CCSGG 3 cut(s) 91, 458, 459
BcoDI GTCTC 2 cut(s) 190, 296
BfaI CTAG 3 cut(s) 53, 363, 473
BfmI CTRYAG 2 cut(s) 81, 295
BisI GCNGC 4 cut(s) 272, 275, 597, 604
BlsI GCNGC 4 cut(s) 273, 276, 598, 605
Bme1390I CCNGG 4 cut(s) 91, 382, 458, 459
Bme18I GGWCC 2 cut(s) 72, 300
BmeT110I CYCGRG 2 cut(s) 313, 457
BmgT120I GGNCC 4 cut(s) 72, 256, 300, 496
BmiI GGNNCC 2 cut(s) 359, 497
BmrFI CCNGG 4 cut(s) 91, 382, 458, 459
BmrI ACTGGG 1 cut(s) 263
BmsI GCATC 1 cut(s) 583
BmuI ACTGGG 1 cut(s) 263
BpiI GAAGAC 2 cut(s) 128, 508
BpmI CTGGAG 2 cut(s) 200, 224
BpuMI CCSGG 3 cut(s) 91, 458, 459
BsaHI GRCGYC 1 cut(s) 414
BsaI GGTCTC 1 cut(s) 296
BsaJI CCNNGG 2 cut(s) 22, 457
BsaWI WCCGGW 1 cut(s) 69
Bsc4I CCNNNNNNNGG 6 cut(s) 69, 253, 254, 287, 343, 463
Bse118I RCCGGY 3 cut(s) 157, 467, 599
Bse1I ACTGG 3 cut(s) 217, 241, 258
BseAI TCCGGA 1 cut(s) 69
BseBI CCWGG 1 cut(s) 382
BseDI CCNNGG 2 cut(s) 22, 457
BseGI GGATG 2 cut(s) 9, 303
BseLI CCNNNNNNNGG 6 cut(s) 69, 253, 254, 287, 343, 463
BseNI ACTGG 3 cut(s) 217, 241, 258
BseRI GAGGAG 2 cut(s) 8, 425
BseX3I CGGCCG 1 cut(s) 601
BseXI GCAGC 1 cut(s) 261
Bsh1285I CGRYCG 3 cut(s) 95, 226, 604
BshFI GGCC 7 cut(s) 5, 27, 161, 258, 467, 498, 603
BsiEI CGRYCG 3 cut(s) 95, 226, 604
BsiHKCI CYCGRG 2 cut(s) 313, 457
BsiSI CCGG 7 cut(s) 70, 91, 144, 158, 458, 468, 600
BslI CCNNNNNNNGG 6 cut(s) 69, 253, 254, 287, 343, 463
BsmAI GTCTC 2 cut(s) 190, 296
BsmBI CGTCTC 1 cut(s) 190
BsnI GGCC 7 cut(s) 5, 27, 161, 258, 467, 498, 603
Bso31I GGTCTC 1 cut(s) 296
BsoBI CYCGRG 2 cut(s) 313, 457
Bsp13I TCCGGA 1 cut(s) 69
Bsp143I GATC 2 cut(s) 166, 447
BspACI CCGC 4 cut(s) 180, 272, 597, 604
BspANI GGCC 7 cut(s) 5, 27, 161, 258, 467, 498, 603
BspEI TCCGGA 1 cut(s) 69
BspLI GGNNCC 2 cut(s) 359, 497
BspPI GGATC 1 cut(s) 455
BspTNI GGTCTC 1 cut(s) 296
BsrFI RCCGGY 3 cut(s) 157, 467, 599
BsrI ACTGG 3 cut(s) 217, 241, 258
BssAI RCCGGY 3 cut(s) 157, 467, 599
BssECI CCNNGG 2 cut(s) 22, 457
BssMI GATC 2 cut(s) 166, 447
BssNI GRCGYC 1 cut(s) 414
Bst2UI CCWGG 1 cut(s) 382
Bst4CI ACNGT 1 cut(s) 224
Bst6I CTCTTC 1 cut(s) 269
BstACI GRCGYC 1 cut(s) 414
BstC8I GCNNGC 4 cut(s) 159, 399, 601, 608
BstDSI CCRYGG 1 cut(s) 22
BstF5I GGATG 2 cut(s) 9, 303
BstKTI GATC 2 cut(s) 169, 450
BstMAI GTCTC 2 cut(s) 190, 296
BstMBI GATC 2 cut(s) 166, 447
BstMCI CGRYCG 3 cut(s) 95, 226, 604
BstMWI GCNNNNNNNGC 3 cut(s) 332, 398, 596
BstNI CCWGG 1 cut(s) 382
BstNSI RCATGY 1 cut(s) 139
BstSCI CCNGG 4 cut(s) 89, 380, 456, 457
BstSFI CTRYAG 2 cut(s) 81, 295
BstV1I GCAGC 1 cut(s) 261
BstV2I GAAGAC 2 cut(s) 128, 508
BstX2I RGATCY 1 cut(s) 447
BstYI RGATCY 1 cut(s) 447
BstZI CGGCCG 1 cut(s) 601
BsuRI GGCC 7 cut(s) 5, 27, 161, 258, 467, 498, 603
BtgI CCRYGG 1 cut(s) 22
BtsCI GGATG 2 cut(s) 9, 303
BtsIMutI CAGTG 1 cut(s) 526
Cac8I GCNNGC 4 cut(s) 159, 399, 601, 608
Cfr10I RCCGGY 3 cut(s) 157, 467, 599
Cfr13I GGNCC 4 cut(s) 72, 256, 300, 496
Cfr9I CCCGGG 1 cut(s) 457
Csp6I GTAC 1 cut(s) 220
CviAII CATG 2 cut(s) 136, 487
CviQI GTAC 1 cut(s) 220
DpnI GATC 2 cut(s) 168, 449
DpnII GATC 2 cut(s) 166, 447
EaeI YGGCCR 2 cut(s) 159, 601
EagI CGGCCG 1 cut(s) 601
Eam1104I CTCTTC 1 cut(s) 269
EarI CTCTTC 1 cut(s) 269
EciI GGCGGA 1 cut(s) 169
EclXI CGGCCG 1 cut(s) 601
Eco31I GGTCTC 1 cut(s) 296
Eco47I GGWCC 2 cut(s) 72, 300
Eco52I CGGCCG 1 cut(s) 601
Eco57I CTGAAG 3 cut(s) 352, 436, 502
Eco88I CYCGRG 2 cut(s) 313, 457
EcoRII CCWGG 1 cut(s) 380
Esp3I CGTCTC 1 cut(s) 190
FaeI CATG 2 cut(s) 139, 490
FaiI YATR 7 cut(s) 83, 137, 190, 395, 488, 501, 540
FatI CATG 2 cut(s) 135, 486
Fnu4HI GCNGC 4 cut(s) 272, 275, 597, 604
FokI GGATG 1 cut(s) 290
Fsp4HI GCNGC 4 cut(s) 272, 275, 597, 604
FspBI CTAG 3 cut(s) 53, 363, 473
GluI GCNGC 4 cut(s) 272, 275, 597, 604
GsuI CTGGAG 2 cut(s) 200, 224
HaeIII GGCC 7 cut(s) 5, 27, 161, 258, 467, 498, 603
HapII CCGG 7 cut(s) 70, 91, 144, 158, 458, 468, 600
Hin1I GRCGYC 1 cut(s) 414
Hin1II CATG 2 cut(s) 139, 490
HinfI GANTC 1 cut(s) 66
HpaII CCGG 7 cut(s) 70, 91, 144, 158, 458, 468, 600
Hpy188I TCNGA 2 cut(s) 444, 614
Hpy188III TCNNGA 2 cut(s) 70, 355
Hpy99I CGWCG 1 cut(s) 326
HpyAV CCTTC 3 cut(s) 211, 269, 337
HpyCH4III ACNGT 1 cut(s) 224
HpyCH4IV ACGT 1 cut(s) 414
HpyCH4V TGCA 2 cut(s) 135, 397
HpyF10VI GCNNNNNNNGC 3 cut(s) 332, 398, 596
HpySE526I ACGT 1 cut(s) 414
Hsp92I GRCGYC 1 cut(s) 414
Hsp92II CATG 2 cut(s) 139, 490
Kpn2I TCCGGA 1 cut(s) 69
KroI GCCGGC 2 cut(s) 157, 599
KroNI GCCGGC 2 cut(s) 159, 601
Kzo9I GATC 2 cut(s) 166, 447
Lsp1109I GCAGC 1 cut(s) 261
LweI GCATC 1 cut(s) 583
MaeI CTAG 3 cut(s) 53, 363, 473
MaeII ACGT 1 cut(s) 414
MaeIII GTNAC 1 cut(s) 403
MalI GATC 2 cut(s) 168, 449
MboI GATC 2 cut(s) 166, 447
MboII GAAGA 6 cut(s) 133, 196, 256, 322, 442, 508
MflI RGATCY 1 cut(s) 447
MluCI AATT 1 cut(s) 208
MroI TCCGGA 1 cut(s) 69
MroNI GCCGGC 2 cut(s) 157, 599
MseI TTAA 1 cut(s) 173
MslI CAYNNNNRTG 1 cut(s) 134
MspA1I CMGCKG 1 cut(s) 274
MspI CCGG 7 cut(s) 70, 91, 144, 158, 458, 468, 600
MspR9I CCNGG 4 cut(s) 91, 382, 458, 459
MvaI CCWGG 1 cut(s) 382
MwoI GCNNNNNNNGC 3 cut(s) 332, 398, 596
NaeI GCCGGC 2 cut(s) 159, 601
NciI CCSGG 3 cut(s) 91, 458, 459
NdeII GATC 2 cut(s) 166, 447
NgoMIV GCCGGC 2 cut(s) 157, 599
NlaIII CATG 2 cut(s) 139, 490
NlaIV GGNNCC 2 cut(s) 359, 497
NspI RCATGY 1 cut(s) 139
PaeR7I CTCGAG 1 cut(s) 313
PdiI GCCGGC 2 cut(s) 159, 601
PfeI GAWTC 1 cut(s) 66
PkrI GCNGC 4 cut(s) 273, 276, 598, 605
Psp6I CCWGG 1 cut(s) 380
PspGI CCWGG 1 cut(s) 380
PspN4I GGNNCC 2 cut(s) 359, 497
PspPI GGNCC 4 cut(s) 72, 256, 300, 496
PspXI VCTCGAGB 1 cut(s) 313
PsuI RGATCY 1 cut(s) 447
RsaI GTAC 1 cut(s) 221
RsaNI GTAC 1 cut(s) 220
RseI CAYNNNNRTG 1 cut(s) 134
SaqAI TTAA 1 cut(s) 173
SatI GCNGC 4 cut(s) 272, 275, 597, 604
Sau3AI GATC 2 cut(s) 166, 447
Sau96I GGNCC 4 cut(s) 72, 256, 300, 496
ScrFI CCNGG 4 cut(s) 91, 382, 458, 459
SetI ASST 9 cut(s) 77, 234, 302, 320, 368, 386, 417, 486, 612
SfaNI GCATC 1 cut(s) 583
SfcI CTRYAG 2 cut(s) 81, 295
Sfr274I CTCGAG 1 cut(s) 313
SinI GGWCC 2 cut(s) 72, 300
SlaI CTCGAG 1 cut(s) 313
SmaI CCCGGG 1 cut(s) 459
SmiMI CAYNNNNRTG 1 cut(s) 134
SmlI CTYRAG 1 cut(s) 313
SmoI CTYRAG 1 cut(s) 313
Sse9I AATT 1 cut(s) 208
SsiI CCGC 4 cut(s) 180, 272, 597, 604
SspMI CTAG 3 cut(s) 53, 363, 473
StyD4I CCNGG 4 cut(s) 89, 380, 456, 457
TaaI ACNGT 1 cut(s) 224
TaiI ACGT 1 cut(s) 417
TaqI TCGA 3 cut(s) 119, 267, 314
TasI AATT 1 cut(s) 208
TauI GCSGC 3 cut(s) 274, 599, 606
TfiI GAWTC 1 cut(s) 66
Tru1I TTAA 1 cut(s) 173
Tru9I TTAA 1 cut(s) 173
TscAI CASTG 1 cut(s) 526
TseI GCWGC 1 cut(s) 274
TspMI CCCGGG 1 cut(s) 457
TspRI CASTG 1 cut(s) 526
VpaK11BI GGWCC 2 cut(s) 72, 300
XceI RCATGY 1 cut(s) 139
XhoI CTCGAG 1 cut(s) 313
XmaI CCCGGG 1 cut(s) 457
XspI CTAG 3 cut(s) 53, 363, 473
ZraI GACGTC 1 cut(s) 415
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.