AT3G03000

calcium-binding protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
3
OG0017085
Physical Location & Seq
Forward (+)
677240 .. 678180
941 bp
Loading structure...
UTR
Exon/CDS
Intron
AT3G03000.1

Sequence Viewer

Length: 498 bp
ATGAGCTGCGACGGAGGCAAACCGGCGCCGGCGAAACTAGGCGACGAACAACTAGCGGAGCTCCGGGAGATATTCCGATCATTTGACCAGAACAAGGATGGAAGTTTGACGGAGCTCGAGTTAGGCTCACTTCTAAGATCTCTCGGTCTAAAGCCGAGTCAAGACCAACTCGACACATTGATCCAGAAAGCAGATCGGAATAACAACGGACTGGTCGAGTTCTCCGAGTTCGTCGCCCTCGTCGAGCCAGATCTGGTCAAGTGTCCTTACACGGATGATCAGCTTAAAGCCATCTTTAGAATGTTTGACCGCGATGGAAACGGTTACATAACGGCGGCGGAGTTAGCCCATTCGATGGCGAAGCTAGGTCACGCGTTGACGGCGGAGGAGTTAACGGGAATGATCAAAGAAGCTGATCGAGACGGCGATGGTTGTATTGATTTCCAAGAGTTTGTTCAAGCGATTACTTCAGCTGCGTTTGATAATGCTTGGGGTTGA

Protein Analysis

165

Amino Acids

18.09

Weight (kDa)

4.41

Isoelectric Point (pI)

33.06

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EF-hand_7 PF13499 20 - 81 2.6e-16 EF-hand domain pair
EF-hand_1 PF00036 20 - 48 3.6e-06 EF hand domain
EF-hand_6 PF13405 20 - 49 1e-07 EF-hand domain
EF-hand_7 PF13499 93 - 155 4e-16 EF-hand domain pair
EF-hand_6 PF13405 94 - 123 8.5e-08 EF-hand domain
EF-hand_1 PF00036 95 - 122 9.3e-08 EF hand domain
EF-hand_8 PF13833 108 - 154 9.5e-11 EF-hand domain pair
EF-hand_1 PF00036 132 - 157 5.7e-08 EF hand domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0017085)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G03000
fragaria_vesca FvH4_7g34050
malus_domestica MD01G1013200.v1.1
pyrus_communis pycom01g04530
rosa_chinensis RchiOBHm_Chr1g0384321
rosa_laevigata RLG00000026049
rosa_multiflora Rmu_co8218092.1_g000001 Rmu_sc0016737.1_g000003 Rmu_sc0018832.1_g000002
rosa_roxburghii Rroxscaffold_4G00276780
rosa_samantha Rh1BG430000 Rh1DG462600
rosa_wichuraiana Rw1G040040

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 25
AccB7I CCANNNNNTGG 1 cut(s) 355
AccII CGCG 2 cut(s) 312, 374
AciI CCGC 5 cut(s) 56, 310, 335, 338, 383
AclWI GGATC 1 cut(s) 175
AcuI CTGAAG 1 cut(s) 453
AcyI GRCGYC 1 cut(s) 26
AfiI CCNNNNNNNGG 2 cut(s) 94, 355
AflIII ACRYGT 1 cut(s) 372
AgsI TTSAA 1 cut(s) 458
AjuI GAANNNNNNNTTGG 2 cut(s) 438, 470
AluBI AGCT 7 cut(s) 6, 61, 115, 283, 364, 413, 473
AluI AGCT 7 cut(s) 6, 61, 115, 283, 364, 413, 473
Alw21I GWGCWC 2 cut(s) 63, 117
Alw26I GTCTC 1 cut(s) 414
AlwI GGATC 1 cut(s) 175
Ama87I CYCGRG 1 cut(s) 116
ApeKI GCWGC 2 cut(s) 6, 473
AspLEI GCGC 1 cut(s) 28
AsuC2I CCSGG 1 cut(s) 65
AvaI CYCGRG 1 cut(s) 116
BanI GGYRCC 1 cut(s) 25
BanII GRGCYC 2 cut(s) 63, 117
Bbv12I GWGCWC 2 cut(s) 63, 117
BbvI GCAGC 1 cut(s) 460
BccI CCATC 5 cut(s) 92, 299, 308, 349, 422
BceAI ACGGC 3 cut(s) 348, 396, 439
BclI TGATCA 2 cut(s) 277, 402
BcnI CCSGG 1 cut(s) 65
BcoDI GTCTC 1 cut(s) 414
BfaI CTAG 3 cut(s) 38, 53, 365
BfoI RGCGCY 1 cut(s) 29
BglII AGATCT 2 cut(s) 137, 250
BisI GCNGC 3 cut(s) 7, 336, 474
BlsI GCNGC 3 cut(s) 8, 337, 475
Bme1390I CCNGG 1 cut(s) 65
BmeT110I CYCGRG 1 cut(s) 116
BmiI GGNNCC 1 cut(s) 27
BmrFI CCNGG 1 cut(s) 65
BplI GAGNNNNNCTC 2 cut(s) 110, 142
BpuMI CCSGG 1 cut(s) 65
BsaHI GRCGYC 1 cut(s) 26
BsaXI ACNNNNNCTCC 2 cut(s) 104, 134
Bsc4I CCNNNNNNNGG 2 cut(s) 94, 355
Bse118I RCCGGY 2 cut(s) 22, 28
Bse1I ACTGG 1 cut(s) 216
BseGI GGATG 2 cut(s) 103, 280
BseLI CCNNNNNNNGG 2 cut(s) 94, 355
BseNI ACTGG 1 cut(s) 216
BseRI GAGGAG 1 cut(s) 401
BseXI GCAGC 1 cut(s) 460
Bsh1236I CGCG 2 cut(s) 312, 374
BshNI GGYRCC 1 cut(s) 25
BsiHKAI GWGCWC 2 cut(s) 63, 117
BsiHKCI CYCGRG 1 cut(s) 116
BsiSI CCGG 3 cut(s) 23, 29, 64
BslI CCNNNNNNNGG 2 cut(s) 94, 355
BsmAI GTCTC 1 cut(s) 414
BsmBI CGTCTC 1 cut(s) 414
BsoBI CYCGRG 1 cut(s) 116
Bsp1286I GDGCHC 2 cut(s) 63, 117
Bsp143I GATC 8 cut(s) 77, 137, 180, 193, 250, 277, 402, 415
BspACI CCGC 5 cut(s) 56, 310, 335, 338, 383
BspFNI CGCG 2 cut(s) 312, 374
BspLI GGNNCC 1 cut(s) 27
BspPI GGATC 1 cut(s) 175
BspT107I GGYRCC 1 cut(s) 25
BsrFI RCCGGY 2 cut(s) 22, 28
BsrI ACTGG 1 cut(s) 216
BssAI RCCGGY 2 cut(s) 22, 28
BssMI GATC 8 cut(s) 77, 137, 180, 193, 250, 277, 402, 415
BssNI GRCGYC 1 cut(s) 26
Bst4CI ACNGT 1 cut(s) 323
BstACI GRCGYC 1 cut(s) 26
BstC8I GCNNGC 1 cut(s) 30
BstDEI CTNAG 1 cut(s) 134
BstF5I GGATG 2 cut(s) 103, 280
BstFNI CGCG 2 cut(s) 312, 374
BstH2I RGCGCY 1 cut(s) 29
BstHHI GCGC 1 cut(s) 28
BstKTI GATC 8 cut(s) 80, 140, 183, 196, 253, 280, 405, 418
BstMAI GTCTC 1 cut(s) 414
BstMBI GATC 8 cut(s) 77, 137, 180, 193, 250, 277, 402, 415
BstMWI GCNNNNNNNGC 3 cut(s) 15, 344, 380
BstSCI CCNGG 1 cut(s) 63
BstUI CGCG 2 cut(s) 312, 374
BstV1I GCAGC 1 cut(s) 460
BstX2I RGATCY 2 cut(s) 137, 250
BstYI RGATCY 2 cut(s) 137, 250
BtgZI GCGATG 2 cut(s) 327, 441
BtsCI GGATG 2 cut(s) 103, 280
Cac8I GCNNGC 1 cut(s) 30
CfoI GCGC 1 cut(s) 28
Cfr10I RCCGGY 2 cut(s) 22, 28
DdeI CTNAG 1 cut(s) 134
DinI GGCGCC 1 cut(s) 27
DpnI GATC 8 cut(s) 79, 139, 182, 195, 252, 279, 404, 417
DpnII GATC 8 cut(s) 77, 137, 180, 193, 250, 277, 402, 415
EciI GGCGGA 2 cut(s) 353, 398
Ecl136II GAGCTC 2 cut(s) 61, 115
Eco24I GRGCYC 2 cut(s) 63, 117
Eco53kI GAGCTC 2 cut(s) 61, 115
Eco57I CTGAAG 1 cut(s) 453
Eco88I CYCGRG 1 cut(s) 116
EcoICRI GAGCTC 2 cut(s) 61, 115
EcoT38I GRGCYC 2 cut(s) 63, 117
EgeI GGCGCC 1 cut(s) 27
EheI GGCGCC 1 cut(s) 27
Esp3I CGTCTC 1 cut(s) 414
FaiI YATR 1 cut(s) 329
FbaI TGATCA 2 cut(s) 277, 402
Fnu4HI GCNGC 3 cut(s) 7, 336, 474
FokI GGATG 2 cut(s) 110, 287
FriOI GRGCYC 2 cut(s) 63, 117
Fsp4HI GCNGC 3 cut(s) 7, 336, 474
FspBI CTAG 3 cut(s) 38, 53, 365
GlaI GCGC 1 cut(s) 27
GluI GCNGC 3 cut(s) 7, 336, 474
HaeII RGCGCY 1 cut(s) 29
HapII CCGG 3 cut(s) 23, 29, 64
HhaI GCGC 1 cut(s) 28
Hin1I GRCGYC 1 cut(s) 26
Hin6I GCGC 1 cut(s) 26
HinP1I GCGC 1 cut(s) 26
HincII GTYRAC 2 cut(s) 378, 393
HindII GTYRAC 2 cut(s) 378, 393
HinfI GANTC 1 cut(s) 157
HpaI GTTAAC 1 cut(s) 393
HpaII CCGG 3 cut(s) 23, 29, 64
Hpy166II GTNNAC 2 cut(s) 378, 393
Hpy188I TCNGA 3 cut(s) 77, 198, 226
Hpy188III TCNNGA 3 cut(s) 161, 184, 419
Hpy8I GTNNAC 2 cut(s) 378, 393
Hpy99I CGWCG 4 cut(s) 14, 47, 236, 245
HpyCH4III ACNGT 1 cut(s) 323
HpyF10VI GCNNNNNNNGC 3 cut(s) 15, 344, 380
HpyF3I CTNAG 1 cut(s) 134
Hsp92I GRCGYC 1 cut(s) 26
HspAI GCGC 1 cut(s) 26
KasI GGCGCC 1 cut(s) 25
KroI GCCGGC 1 cut(s) 28
KroNI GCCGGC 1 cut(s) 30
Ksp22I TGATCA 2 cut(s) 277, 402
KspAI GTTAAC 1 cut(s) 393
Kzo9I GATC 8 cut(s) 77, 137, 180, 193, 250, 277, 402, 415
LmnI GCTCC 3 cut(s) 58, 66, 112
LpnPI CCDG 8 cut(s) 36, 42, 77, 101, 197, 197, 239, 261
Lsp1109I GCAGC 1 cut(s) 460
MaeI CTAG 3 cut(s) 38, 53, 365
MaeIII GTNAC 2 cut(s) 323, 368
MalI GATC 8 cut(s) 79, 139, 182, 195, 252, 279, 404, 417
MboI GATC 8 cut(s) 77, 137, 180, 193, 250, 277, 402, 415
MflI RGATCY 2 cut(s) 137, 250
MhlI GDGCHC 2 cut(s) 63, 117
MluI ACGCGT 1 cut(s) 372
Mly113I GGCGCC 1 cut(s) 26
MlyI GAGTC 1 cut(s) 166
MnlI CCTC 3 cut(s) 8, 248, 379
MreI CGCCGGCG 1 cut(s) 28
MroNI GCCGGC 1 cut(s) 28
MseI TTAA 2 cut(s) 285, 392
MspA1I CMGCKG 1 cut(s) 473
MspI CCGG 3 cut(s) 23, 29, 64
MspR9I CCNGG 1 cut(s) 65
MvnI CGCG 2 cut(s) 312, 374
MwoI GCNNNNNNNGC 3 cut(s) 15, 344, 380
NaeI GCCGGC 1 cut(s) 30
NarI GGCGCC 1 cut(s) 26
NciI CCSGG 1 cut(s) 65
NdeII GATC 8 cut(s) 77, 137, 180, 193, 250, 277, 402, 415
NgoMIV GCCGGC 1 cut(s) 28
NlaIV GGNNCC 1 cut(s) 27
NmeAIII GCCGAG 1 cut(s) 180
NmuCI GTSAC 1 cut(s) 368
PaeR7I CTCGAG 1 cut(s) 116
PcsI WCGNNNNNNNCGW 4 cut(s) 213, 222, 237, 240
PdiI GCCGGC 1 cut(s) 30
PflMI CCANNNNNTGG 1 cut(s) 355
PfoI TCCNGGA 1 cut(s) 63
PkrI GCNGC 3 cut(s) 8, 337, 475
PleI GAGTC 1 cut(s) 165
PluTI GGCGCC 1 cut(s) 29
PpsI GAGTC 1 cut(s) 165
Psp124BI GAGCTC 2 cut(s) 63, 117
PspN4I GGNNCC 1 cut(s) 27
PspXI VCTCGAGB 1 cut(s) 116
PsuI RGATCY 2 cut(s) 137, 250
PvuII CAGCTG 1 cut(s) 473
SacI GAGCTC 2 cut(s) 63, 117
SaqAI TTAA 2 cut(s) 285, 392
SatI GCNGC 3 cut(s) 7, 336, 474
Sau3AI GATC 8 cut(s) 77, 137, 180, 193, 250, 277, 402, 415
SchI GAGTC 1 cut(s) 166
ScrFI CCNGG 1 cut(s) 65
SduI GDGCHC 2 cut(s) 63, 117
SetI ASST 8 cut(s) 8, 63, 117, 285, 366, 370, 415, 475
SfoI GGCGCC 1 cut(s) 27
Sfr274I CTCGAG 1 cut(s) 116
SgrAI CRCCGGYG 1 cut(s) 28
SlaI CTCGAG 1 cut(s) 116
SmlI CTYRAG 1 cut(s) 116
SmoI CTYRAG 1 cut(s) 116
SsiI CCGC 5 cut(s) 56, 310, 335, 338, 383
SspDI GGCGCC 1 cut(s) 25
SspMI CTAG 3 cut(s) 38, 53, 365
SstI GAGCTC 2 cut(s) 63, 117
StyD4I CCNGG 1 cut(s) 63
TaaI ACNGT 1 cut(s) 323
TaqI TCGA 6 cut(s) 117, 171, 216, 243, 353, 418
TaqII GACCGA 1 cut(s) 134
TauI GCSGC 1 cut(s) 338
Tru1I TTAA 2 cut(s) 285, 392
Tru9I TTAA 2 cut(s) 285, 392
TseFI GTSAC 1 cut(s) 368
TseI GCWGC 2 cut(s) 6, 473
Tsp45I GTSAC 1 cut(s) 368
TspGWI ACGGA 4 cut(s) 27, 125, 222, 287
Van91I CCANNNNNTGG 1 cut(s) 355
XcmI CCANNNNNNNNNTGG 1 cut(s) 95
XhoI CTCGAG 1 cut(s) 116
XspI CTAG 3 cut(s) 38, 53, 365
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.