pycom01g04530

calcium-binding protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr1
OG0017085
Physical Location & Seq
Forward (+)
3385064 .. 3385552
489 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom01g04530.1

Sequence Viewer

Length: 489 bp
ATGGAGGAGGAGCAGATGGCACAGCTGCGCGAGATCTTCCGATCGTTTGACGGCAACAAAGACGGCAGCTTGACCGAGCCGGAGTTGGGGTCACTGCTGCTGTCGCTTGGGCTGAAGCCGAGCCATCAGCAGCTGCAGGCTCTGATTCGAAGGTCGGATACCAACCACAACGGATTGGTCGGGTTCTCGGAGTTCGTGGCGCTGATGAGGCCGGACCTCAACGGACTCTTACAAGACGACGCCAGGTGCCCGTGTTCGTACAGTGACGATCAGTTGAGGCAGATGTTCAGCATGTTCGACAGGGACGCCAACGGGTACATCAGCCCGGCGGAACTGGCCCACTCGATGGCCAAGCTGGGCCACCCACTCACGGTTCAGGAGTTGTTCGGCATGATGAACGAGGCGGATACCGACCGCGATGGCCGTATCGATTTTCACGAGTTTTCCAGGGCCATCACCTCAGCCGCTTTCCTTAATATCAATGCTTAA

Protein Analysis

163

Amino Acids

18.17

Weight (kDa)

4.73

Isoelectric Point (pI)

45.59

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EF-hand_7 PF13499 7 - 70 5e-15 EF-hand domain pair
EF-hand_6 PF13405 8 - 37 7.6e-06 EF-hand domain
EF-hand_7 PF13499 90 - 152 4e-15 EF-hand domain pair
EF-hand_6 PF13405 91 - 120 4.5e-06 EF-hand domain
EF-hand_1 PF00036 92 - 119 2e-06 EF hand domain
EF-hand_8 PF13833 104 - 150 3.8e-10 EF-hand domain pair
EF-hand_1 PF00036 128 - 154 1.2e-06 EF hand domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017085)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G03000
fragaria_vesca FvH4_7g34050
malus_domestica MD01G1013200.v1.1
pyrus_communis pycom01g04530
rosa_chinensis RchiOBHm_Chr1g0384321
rosa_laevigata RLG00000026049
rosa_multiflora Rmu_co8218092.1_g000001 Rmu_sc0016737.1_g000003 Rmu_sc0018832.1_g000002
rosa_roxburghii Rroxscaffold_4G00276780
rosa_samantha Rh1BG430000 Rh1DG462600
rosa_wichuraiana Rw1G040040

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 246
AccB7I CCANNNNNTGG 1 cut(s) 346
AccII CGCG 2 cut(s) 30, 417
AciI CCGC 4 cut(s) 329, 404, 415, 465
AcoI YGGCCR 2 cut(s) 348, 421
AcuI CTGAAG 1 cut(s) 134
AcyI GRCGYC 2 cut(s) 240, 306
AfaI GTAC 2 cut(s) 260, 317
AfiI CCNNNNNNNGG 3 cut(s) 86, 346, 370
AjnI CCWGG 2 cut(s) 242, 446
AluBI AGCT 4 cut(s) 25, 69, 133, 355
AluI AGCT 4 cut(s) 25, 69, 133, 355
AlwNI CAGNNNCTG 2 cut(s) 133, 142
AoxI GGCC 6 cut(s) 209, 336, 348, 358, 421, 450
ApeKI GCWGC 5 cut(s) 25, 66, 97, 130, 133
AspLEI GCGC 2 cut(s) 30, 202
AspS9I GGNCC 4 cut(s) 214, 337, 358, 450
AsuC2I CCSGG 1 cut(s) 326
AsuHPI GGTGA 1 cut(s) 448
AsuII TTCGAA 1 cut(s) 148
AvaII GGWCC 1 cut(s) 214
BaeGI GKGCMC 1 cut(s) 251
BalI TGGCCA 1 cut(s) 350
BanI GGYRCC 1 cut(s) 246
BarI GAAGNNNNNNTAC 2 cut(s) 142, 174
BauI CACGAG 1 cut(s) 437
BbvCI CCTCAGC 1 cut(s) 460
BbvI GCAGC 5 cut(s) 12, 78, 84, 120, 142
BccI CCATC 5 cut(s) 10, 132, 340, 413, 461
BceAI ACGGC 3 cut(s) 67, 79, 408
BciT130I CCWGG 2 cut(s) 244, 448
BciVI GTATCC 2 cut(s) 151, 400
BcnI CCSGG 1 cut(s) 326
BfmI CTRYAG 1 cut(s) 134
BfoI RGCGCY 1 cut(s) 203
BfuI GTATCC 2 cut(s) 151, 400
BglII AGATCT 1 cut(s) 33
BisI GCNGC 6 cut(s) 26, 67, 98, 131, 134, 465
BlsI GCNGC 6 cut(s) 27, 68, 99, 132, 135, 466
Bme1390I CCNGG 3 cut(s) 244, 326, 448
Bme18I GGWCC 1 cut(s) 214
BmgT120I GGNCC 4 cut(s) 214, 337, 358, 450
BmiI GGNNCC 1 cut(s) 248
BmrFI CCNGG 3 cut(s) 244, 326, 448
Bpu10I CCTNAGC 1 cut(s) 460
Bpu14I TTCGAA 1 cut(s) 148
BpuMI CCSGG 1 cut(s) 326
Bsa29I ATCGAT 1 cut(s) 429
BsaHI GRCGYC 2 cut(s) 240, 306
BsaJI CCNNGG 1 cut(s) 447
BsaXI ACNNNNNCTCC 2 cut(s) 74, 104
Bsc4I CCNNNNNNNGG 3 cut(s) 86, 346, 370
Bse1I ACTGG 1 cut(s) 339
BseBI CCWGG 2 cut(s) 244, 448
BseCI ATCGAT 1 cut(s) 429
BseDI CCNNGG 1 cut(s) 447
BseLI CCNNNNNNNGG 3 cut(s) 86, 346, 370
BseMII CTCAG 1 cut(s) 474
BseNI ACTGG 1 cut(s) 339
BseRI GAGGAG 2 cut(s) 20, 23
BseSI GKGCMC 1 cut(s) 251
BseXI GCAGC 5 cut(s) 12, 78, 84, 120, 142
BseYI CCCAGC 1 cut(s) 355
Bsh1236I CGCG 2 cut(s) 30, 417
Bsh1285I CGRYCG 2 cut(s) 44, 415
BshFI GGCC 6 cut(s) 211, 338, 350, 360, 423, 452
BshNI GGYRCC 1 cut(s) 246
BshVI ATCGAT 1 cut(s) 429
BsiEI CGRYCG 2 cut(s) 44, 415
BsiSI CCGG 3 cut(s) 80, 212, 326
BslFI GGGAC 1 cut(s) 317
BslI CCNNNNNNNGG 3 cut(s) 86, 346, 370
BsmFI GGGAC 1 cut(s) 317
BsnI GGCC 6 cut(s) 211, 338, 350, 360, 423, 452
Bsp119I TTCGAA 1 cut(s) 148
Bsp1286I GDGCHC 1 cut(s) 251
Bsp143I GATC 3 cut(s) 33, 41, 268
BspACI CCGC 4 cut(s) 329, 404, 415, 465
BspANI GGCC 6 cut(s) 211, 338, 350, 360, 423, 452
BspCNI CTCAG 1 cut(s) 473
BspDI ATCGAT 1 cut(s) 429
BspFNI CGCG 2 cut(s) 30, 417
BspLI GGNNCC 1 cut(s) 248
BspMAI CTGCAG 1 cut(s) 138
BspT104I TTCGAA 1 cut(s) 148
BspT107I GGYRCC 1 cut(s) 246
BsrI ACTGG 1 cut(s) 339
BssECI CCNNGG 1 cut(s) 447
BssMI GATC 3 cut(s) 33, 41, 268
BssNI GRCGYC 2 cut(s) 240, 306
BssSI CACGAG 1 cut(s) 437
Bst2BI CACGAG 1 cut(s) 437
Bst2UI CCWGG 2 cut(s) 244, 448
Bst4CI ACNGT 2 cut(s) 263, 373
BstACI GRCGYC 2 cut(s) 240, 306
BstBI TTCGAA 1 cut(s) 148
BstC8I GCNNGC 1 cut(s) 138
BstDEI CTNAG 1 cut(s) 460
BstFNI CGCG 2 cut(s) 30, 417
BstH2I RGCGCY 1 cut(s) 203
BstHHI GCGC 2 cut(s) 30, 202
BstKTI GATC 3 cut(s) 36, 44, 271
BstMBI GATC 3 cut(s) 33, 41, 268
BstMCI CGRYCG 2 cut(s) 44, 415
BstMWI GCNNNNNNNGC 3 cut(s) 103, 208, 335
BstNI CCWGG 2 cut(s) 244, 448
BstNSI RCATGY 1 cut(s) 295
BstSCI CCNGG 3 cut(s) 242, 324, 446
BstSFI CTRYAG 1 cut(s) 134
BstSLI GKGCMC 1 cut(s) 251
BstUI CGCG 2 cut(s) 30, 417
BstV1I GCAGC 5 cut(s) 12, 78, 84, 120, 142
BstX2I RGATCY 1 cut(s) 33
BstYI RGATCY 1 cut(s) 33
Bsu15I ATCGAT 1 cut(s) 429
BsuI GTATCC 2 cut(s) 151, 400
BsuRI GGCC 6 cut(s) 211, 338, 350, 360, 423, 452
BsuTUI ATCGAT 1 cut(s) 429
BtgZI GCGATG 1 cut(s) 432
BtsI GCAGTG 1 cut(s) 92
BtsIMutI CAGTG 2 cut(s) 92, 268
Cac8I GCNNGC 1 cut(s) 138
CaiI CAGNNNCTG 2 cut(s) 133, 142
CfoI GCGC 2 cut(s) 30, 202
Cfr13I GGNCC 4 cut(s) 214, 337, 358, 450
ClaI ATCGAT 1 cut(s) 429
CseI GACGC 2 cut(s) 248, 314
Csp6I GTAC 2 cut(s) 259, 316
CviAII CATG 2 cut(s) 292, 391
CviQI GTAC 2 cut(s) 259, 316
DdeI CTNAG 1 cut(s) 460
DpnI GATC 3 cut(s) 35, 43, 270
DpnII GATC 3 cut(s) 33, 41, 268
EaeI YGGCCR 2 cut(s) 348, 421
EciI GGCGGA 2 cut(s) 344, 419
Eco47I GGWCC 1 cut(s) 214
Eco57I CTGAAG 1 cut(s) 134
EcoRII CCWGG 2 cut(s) 242, 446
FaeI CATG 2 cut(s) 295, 394
FaiI YATR 2 cut(s) 293, 392
FaqI GGGAC 1 cut(s) 317
FatI CATG 2 cut(s) 291, 390
Fnu4HI GCNGC 6 cut(s) 26, 67, 98, 131, 134, 465
Fsp4HI GCNGC 6 cut(s) 26, 67, 98, 131, 134, 465
GlaI GCGC 2 cut(s) 29, 201
GluI GCNGC 6 cut(s) 26, 67, 98, 131, 134, 465
GsaI CCCAGC 1 cut(s) 359
HaeII RGCGCY 1 cut(s) 203
HaeIII GGCC 6 cut(s) 211, 338, 350, 360, 423, 452
HapII CCGG 3 cut(s) 80, 212, 326
HgaI GACGC 2 cut(s) 248, 314
HhaI GCGC 2 cut(s) 30, 202
Hin1I GRCGYC 2 cut(s) 240, 306
Hin1II CATG 2 cut(s) 295, 394
Hin6I GCGC 2 cut(s) 28, 200
HinP1I GCGC 2 cut(s) 28, 200
HinfI GANTC 2 cut(s) 145, 225
HpaII CCGG 3 cut(s) 80, 212, 326
HphI GGTGA 1 cut(s) 448
Hpy188I TCNGA 4 cut(s) 41, 144, 157, 190
Hpy188III TCNNGA 2 cut(s) 377, 437
Hpy99I CGWCG 1 cut(s) 242
HpyAV CCTTC 1 cut(s) 144
HpyCH4III ACNGT 2 cut(s) 263, 373
HpyCH4V TGCA 1 cut(s) 136
HpyF10VI GCNNNNNNNGC 3 cut(s) 103, 208, 335
HpyF3I CTNAG 1 cut(s) 460
Hsp92I GRCGYC 2 cut(s) 240, 306
Hsp92II CATG 2 cut(s) 295, 394
HspAI GCGC 2 cut(s) 28, 200
Kzo9I GATC 3 cut(s) 33, 41, 268
LmnI GCTCC 1 cut(s) 10
Lsp1109I GCAGC 5 cut(s) 12, 78, 84, 120, 142
MaeIII GTNAC 2 cut(s) 90, 263
MalI GATC 3 cut(s) 35, 43, 270
MboI GATC 3 cut(s) 33, 41, 268
MboII GAAGA 1 cut(s) 28
MflI RGATCY 1 cut(s) 33
MhlI GDGCHC 1 cut(s) 251
MlsI TGGCCA 1 cut(s) 350
MluNI TGGCCA 1 cut(s) 350
MlyI GAGTC 1 cut(s) 219
MmeI TCCRAC 1 cut(s) 135
MnlI CCTC 5 cut(s) 201, 227, 270, 394, 469
Mox20I TGGCCA 1 cut(s) 350
MscI TGGCCA 1 cut(s) 350
MseI TTAA 2 cut(s) 474, 487
Msp20I TGGCCA 1 cut(s) 350
MspA1I CMGCKG 2 cut(s) 25, 133
MspI CCGG 3 cut(s) 80, 212, 326
MspR9I CCNGG 3 cut(s) 244, 326, 448
MvaI CCWGG 2 cut(s) 244, 448
MvnI CGCG 2 cut(s) 30, 417
MwoI GCNNNNNNNGC 3 cut(s) 103, 208, 335
NciI CCSGG 1 cut(s) 326
NdeII GATC 3 cut(s) 33, 41, 268
NlaIII CATG 2 cut(s) 295, 394
NlaIV GGNNCC 1 cut(s) 248
NmeAIII GCCGAG 1 cut(s) 144
NmuCI GTSAC 2 cut(s) 90, 263
NspI RCATGY 1 cut(s) 295
NspV TTCGAA 1 cut(s) 148
PcsI WCGNNNNNNNCGW 1 cut(s) 435
PfeI GAWTC 1 cut(s) 145
PflMI CCANNNNNTGG 1 cut(s) 346
PkrI GCNGC 6 cut(s) 27, 68, 99, 132, 135, 466
Ple19I CGATCG 1 cut(s) 44
PleI GAGTC 1 cut(s) 219
PpsI GAGTC 1 cut(s) 219
Psp6I CCWGG 2 cut(s) 242, 446
PspFI CCCAGC 1 cut(s) 355
PspGI CCWGG 2 cut(s) 242, 446
PspN4I GGNNCC 1 cut(s) 248
PspPI GGNCC 4 cut(s) 214, 337, 358, 450
PstI CTGCAG 1 cut(s) 138
PstNI CAGNNNCTG 2 cut(s) 133, 142
PsuI RGATCY 1 cut(s) 33
PvuI CGATCG 1 cut(s) 44
PvuII CAGCTG 2 cut(s) 25, 133
RsaI GTAC 2 cut(s) 260, 317
RsaNI GTAC 2 cut(s) 259, 316
SaqAI TTAA 2 cut(s) 474, 487
SatI GCNGC 6 cut(s) 26, 67, 98, 131, 134, 465
Sau3AI GATC 3 cut(s) 33, 41, 268
Sau96I GGNCC 4 cut(s) 214, 337, 358, 450
SchI GAGTC 1 cut(s) 219
ScrFI CCNGG 3 cut(s) 244, 326, 448
SduI GDGCHC 1 cut(s) 251
SetI ASST 8 cut(s) 27, 71, 135, 155, 219, 248, 357, 461
SfcI CTRYAG 1 cut(s) 134
SfuI TTCGAA 1 cut(s) 148
SinI GGWCC 1 cut(s) 214
SsiI CCGC 4 cut(s) 329, 404, 415, 465
StyD4I CCNGG 3 cut(s) 242, 324, 446
TaaI ACNGT 2 cut(s) 263, 373
TaqI TCGA 4 cut(s) 148, 297, 344, 429
TaqII GACCGA 1 cut(s) 89
TauI GCSGC 1 cut(s) 467
TfiI GAWTC 1 cut(s) 145
Tru1I TTAA 2 cut(s) 474, 487
Tru9I TTAA 2 cut(s) 474, 487
TscAI CASTG 2 cut(s) 99, 268
TseFI GTSAC 2 cut(s) 90, 263
TseI GCWGC 5 cut(s) 25, 66, 97, 130, 133
Tsp45I GTSAC 2 cut(s) 90, 263
TspDTI ATGAA 1 cut(s) 410
TspGWI ACGGA 2 cut(s) 186, 237
TspRI CASTG 2 cut(s) 99, 268
Van91I CCANNNNNTGG 1 cut(s) 346
VpaK11BI GGWCC 1 cut(s) 214
XceI RCATGY 1 cut(s) 295
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.