RLG00000026049

calcium-binding protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr6
OG0017085
Physical Location & Seq
Reverse (-)
241158 .. 241673
516 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000026049

Sequence Viewer

Length: 516 bp
ATGAGCGCCAACAGAAAGCAATCAGCTCTGGCGCCGAAGCTGGACCACGAGCAGATCGCGGAGCTGCGAGAGATATTCCGATCGTTTGACCGAAACAACGACGGAAGTTTGACCCCTCTGGAGCTGGGGTCGCTGCTCCAGTCTCTGGGGCTGAAGGCGAGCCATGAGCAGGTGGAGACTCTGATCCAAAAGGCTGATCGTAACGACAACGGGCTGGTGGAGTTCTCGGAGTTCCTGGTGCTGGTGAAGCCTGACCTACTACTACTGCAAGGCGGCATGTGTCCGTACAGTGAGGAGATGCTGAGGAACATGTTTGACATGTTCGATAGGGATGGTAATGGCTACATCAGTGCGGCGGAGCTGGCCCACTCCATGGCCAAGCTTGGCCACGCGCTCACGGCGGAGGAGCTGATTGGGATGATGGAGGAGGCCGATACGGATGGAGATGGCCAAATCGATTTTCAGGAGTTTGCAAGGGCCATTACATCGGCGGCGGTCCATAATCGTTGGACATGA

Protein Analysis

172

Amino Acids

19.04

Weight (kDa)

4.63

Isoelectric Point (pI)

41.99

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EF-hand_7 PF13499 21 - 79 2.4e-15 EF-hand domain pair
EF-hand_1 PF00036 21 - 49 5.5e-06 EF hand domain
EF-hand_6 PF13405 21 - 50 7.8e-08 EF-hand domain
EF-hand_7 PF13499 98 - 161 1.2e-16 EF-hand domain pair
EF-hand_1 PF00036 101 - 128 1e-07 EF hand domain
EF-hand_6 PF13405 101 - 129 2.4e-07 EF-hand domain
EF-hand_8 PF13833 114 - 159 8.7e-09 EF-hand domain pair
EF-hand_1 PF00036 137 - 163 7.3e-08 EF hand domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017085)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G03000
fragaria_vesca FvH4_7g34050
malus_domestica MD01G1013200.v1.1
pyrus_communis pycom01g04530
rosa_chinensis RchiOBHm_Chr1g0384321
rosa_laevigata RLG00000026049
rosa_multiflora Rmu_co8218092.1_g000001 Rmu_sc0016737.1_g000003 Rmu_sc0018832.1_g000002
rosa_roxburghii Rroxscaffold_4G00276780
rosa_samantha Rh1BG430000 Rh1DG462600
rosa_wichuraiana Rw1G040040

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 160
Acc36I ACCTGC 1 cut(s) 160
AccB1I GGYRCC 1 cut(s) 31
AccB7I CCANNNNNTGG 2 cut(s) 145, 373
AccII CGCG 2 cut(s) 59, 392
AciI CCGC 7 cut(s) 59, 273, 353, 356, 401, 491, 494
AclWI GGATC 1 cut(s) 178
AcoI YGGCCR 3 cut(s) 375, 385, 448
AcuI CTGAAG 1 cut(s) 173
AcyI GRCGYC 1 cut(s) 32
AfaI GTAC 1 cut(s) 287
AfiI CCNNNNNNNGG 4 cut(s) 145, 169, 241, 373
AflIII ACRYGT 2 cut(s) 309, 318
AjnI CCWGG 1 cut(s) 234
AjuI GAANNNNNNNTTGG 2 cut(s) 444, 476
AluBI AGCT 7 cut(s) 26, 40, 64, 124, 361, 382, 409
AluI AGCT 7 cut(s) 26, 40, 64, 124, 361, 382, 409
Alw26I GTCTC 2 cut(s) 147, 170
AlwI GGATC 1 cut(s) 178
AlwNI CAGNNNCTG 1 cut(s) 145
AoxI GGCC 6 cut(s) 363, 375, 385, 429, 448, 477
ApeKI GCWGC 2 cut(s) 64, 133
AspLEI GCGC 3 cut(s) 8, 34, 394
AspS9I GGNCC 4 cut(s) 43, 364, 477, 496
AsuHPI GGTGA 1 cut(s) 256
AvaII GGWCC 2 cut(s) 43, 496
BalI TGGCCA 3 cut(s) 377, 387, 450
BanI GGYRCC 1 cut(s) 31
BauI CACGAG 1 cut(s) 47
BbvCI CCTCAGC 1 cut(s) 302
BbvI GCAGC 2 cut(s) 51, 120
BccI CCATC 4 cut(s) 326, 415, 434, 440
BceAI ACGGC 1 cut(s) 414
BciT130I CCWGG 1 cut(s) 236
BcoDI GTCTC 2 cut(s) 147, 170
BfoI RGCGCY 2 cut(s) 9, 35
BfuAI ACCTGC 1 cut(s) 160
BisI GCNGC 5 cut(s) 65, 134, 274, 354, 492
BlsI GCNGC 5 cut(s) 66, 135, 275, 355, 493
Bme1390I CCNGG 1 cut(s) 236
Bme18I GGWCC 2 cut(s) 43, 496
BmgT120I GGNCC 4 cut(s) 43, 364, 477, 496
BmiI GGNNCC 1 cut(s) 33
BmrFI CCNGG 1 cut(s) 236
BmsI GCATC 1 cut(s) 288
BpmI CTGGAG 2 cut(s) 122, 140
Bpu10I CCTNAGC 1 cut(s) 302
Bsa29I ATCGAT 1 cut(s) 456
BsaHI GRCGYC 1 cut(s) 32
BsaJI CCNNGG 1 cut(s) 372
BsaXI ACNNNNNCTCC 2 cut(s) 113, 143
Bsc4I CCNNNNNNNGG 4 cut(s) 145, 169, 241, 373
Bse1I ACTGG 1 cut(s) 139
BseBI CCWGG 1 cut(s) 236
BseCI ATCGAT 1 cut(s) 456
BseDI CCNNGG 1 cut(s) 372
BseGI GGATG 3 cut(s) 337, 423, 445
BseLI CCNNNNNNNGG 4 cut(s) 145, 169, 241, 373
BseMII CTCAG 1 cut(s) 293
BseNI ACTGG 1 cut(s) 139
BseRI GAGGAG 3 cut(s) 308, 419, 440
BseXI GCAGC 2 cut(s) 51, 120
BseYI CCCAGC 1 cut(s) 124
Bsh1236I CGCG 2 cut(s) 59, 392
Bsh1285I CGRYCG 1 cut(s) 83
BshFI GGCC 6 cut(s) 365, 377, 387, 431, 450, 479
BshNI GGYRCC 1 cut(s) 31
BshVI ATCGAT 1 cut(s) 456
BsiEI CGRYCG 1 cut(s) 83
BslI CCNNNNNNNGG 4 cut(s) 145, 169, 241, 373
BsmAI GTCTC 2 cut(s) 147, 170
BsnI GGCC 6 cut(s) 365, 377, 387, 431, 450, 479
Bsp143I GATC 4 cut(s) 54, 80, 183, 196
Bsp19I CCATGG 1 cut(s) 372
BspACI CCGC 7 cut(s) 59, 273, 353, 356, 401, 491, 494
BspANI GGCC 6 cut(s) 365, 377, 387, 431, 450, 479
BspCNI CTCAG 1 cut(s) 294
BspDI ATCGAT 1 cut(s) 456
BspFNI CGCG 2 cut(s) 59, 392
BspLI GGNNCC 1 cut(s) 33
BspMI ACCTGC 1 cut(s) 160
BspPI GGATC 1 cut(s) 178
BspT107I GGYRCC 1 cut(s) 31
BsrI ACTGG 1 cut(s) 139
BssECI CCNNGG 1 cut(s) 372
BssMI GATC 4 cut(s) 54, 80, 183, 196
BssNI GRCGYC 1 cut(s) 32
BssSI CACGAG 1 cut(s) 47
BssT1I CCWWGG 1 cut(s) 372
Bst2BI CACGAG 1 cut(s) 47
Bst2UI CCWGG 1 cut(s) 236
Bst4CI ACNGT 1 cut(s) 290
BstACI GRCGYC 1 cut(s) 32
BstC8I GCNNGC 2 cut(s) 160, 363
BstDEI CTNAG 1 cut(s) 302
BstDSI CCRYGG 1 cut(s) 372
BstF5I GGATG 3 cut(s) 337, 423, 445
BstFNI CGCG 2 cut(s) 59, 392
BstH2I RGCGCY 2 cut(s) 9, 35
BstHHI GCGC 3 cut(s) 8, 34, 394
BstKTI GATC 4 cut(s) 57, 83, 186, 199
BstMAI GTCTC 2 cut(s) 147, 170
BstMBI GATC 4 cut(s) 54, 80, 183, 196
BstMCI CGRYCG 1 cut(s) 83
BstMWI GCNNNNNNNGC 4 cut(s) 130, 247, 362, 398
BstNI CCWGG 1 cut(s) 236
BstNSI RCATGY 3 cut(s) 280, 313, 322
BstSCI CCNGG 1 cut(s) 234
BstUI CGCG 2 cut(s) 59, 392
BstV1I GCAGC 2 cut(s) 51, 120
Bsu15I ATCGAT 1 cut(s) 456
BsuRI GGCC 6 cut(s) 365, 377, 387, 431, 450, 479
BsuTUI ATCGAT 1 cut(s) 456
BtgI CCRYGG 1 cut(s) 372
BtsCI GGATG 3 cut(s) 337, 423, 445
BtsIMutI CAGTG 2 cut(s) 295, 355
BveI ACCTGC 1 cut(s) 160
Cac8I GCNNGC 2 cut(s) 160, 363
CaiI CAGNNNCTG 1 cut(s) 145
CfoI GCGC 3 cut(s) 8, 34, 394
Cfr13I GGNCC 4 cut(s) 43, 364, 477, 496
ClaI ATCGAT 1 cut(s) 456
Csp6I GTAC 1 cut(s) 286
CviAII CATG 6 cut(s) 164, 277, 310, 319, 373, 513
CviQI GTAC 1 cut(s) 286
DdeI CTNAG 1 cut(s) 302
DinI GGCGCC 1 cut(s) 33
DpnI GATC 4 cut(s) 56, 82, 185, 198
DpnII GATC 4 cut(s) 54, 80, 183, 196
EaeI YGGCCR 3 cut(s) 375, 385, 448
EciI GGCGGA 2 cut(s) 371, 416
Eco130I CCWWGG 1 cut(s) 372
Eco47I GGWCC 2 cut(s) 43, 496
Eco57I CTGAAG 1 cut(s) 173
EcoRII CCWGG 1 cut(s) 234
EcoT14I CCWWGG 1 cut(s) 372
EgeI GGCGCC 1 cut(s) 33
EheI GGCGCC 1 cut(s) 33
ErhI CCWWGG 1 cut(s) 372
FaeI CATG 6 cut(s) 167, 280, 313, 322, 376, 516
FaiI YATR 7 cut(s) 165, 278, 311, 320, 374, 501, 514
FatI CATG 6 cut(s) 163, 276, 309, 318, 372, 512
Fnu4HI GCNGC 5 cut(s) 65, 134, 274, 354, 492
FokI GGATG 3 cut(s) 344, 430, 452
Fsp4HI GCNGC 5 cut(s) 65, 134, 274, 354, 492
GlaI GCGC 3 cut(s) 7, 33, 393
GluI GCNGC 5 cut(s) 65, 134, 274, 354, 492
GsaI CCCAGC 1 cut(s) 128
GsuI CTGGAG 2 cut(s) 122, 140
HaeII RGCGCY 2 cut(s) 9, 35
HaeIII GGCC 6 cut(s) 365, 377, 387, 431, 450, 479
HhaI GCGC 3 cut(s) 8, 34, 394
Hin1I GRCGYC 1 cut(s) 32
Hin1II CATG 6 cut(s) 167, 280, 313, 322, 376, 516
Hin6I GCGC 3 cut(s) 6, 32, 392
HinP1I GCGC 3 cut(s) 6, 32, 392
HindIII AAGCTT 1 cut(s) 380
HinfI GANTC 1 cut(s) 178
HphI GGTGA 1 cut(s) 256
Hpy188I TCNGA 3 cut(s) 80, 183, 229
Hpy188III TCNNGA 2 cut(s) 119, 464
Hpy99I CGWCG 1 cut(s) 104
HpyAV CCTTC 1 cut(s) 148
HpyCH4III ACNGT 1 cut(s) 290
HpyCH4V TGCA 2 cut(s) 268, 473
HpyF10VI GCNNNNNNNGC 4 cut(s) 130, 247, 362, 398
HpyF3I CTNAG 1 cut(s) 302
Hsp92I GRCGYC 1 cut(s) 32
Hsp92II CATG 6 cut(s) 167, 280, 313, 322, 376, 516
HspAI GCGC 3 cut(s) 6, 32, 392
KasI GGCGCC 1 cut(s) 31
Kzo9I GATC 4 cut(s) 54, 80, 183, 196
LmnI GCTCC 5 cut(s) 61, 121, 141, 358, 406
Lsp1109I GCAGC 2 cut(s) 51, 120
LweI GCATC 1 cut(s) 288
MaeIII GTNAC 1 cut(s) 200
MalI GATC 4 cut(s) 56, 82, 185, 198
MboI GATC 4 cut(s) 54, 80, 183, 196
MlsI TGGCCA 3 cut(s) 377, 387, 450
MluNI TGGCCA 3 cut(s) 377, 387, 450
Mly113I GGCGCC 1 cut(s) 32
MlyI GAGTC 1 cut(s) 172
MmeI TCCRAC 1 cut(s) 488
MnlI CCTC 6 cut(s) 126, 286, 297, 397, 418, 421
Mox20I TGGCCA 3 cut(s) 377, 387, 450
MscI TGGCCA 3 cut(s) 377, 387, 450
Msp20I TGGCCA 3 cut(s) 377, 387, 450
MspR9I CCNGG 1 cut(s) 236
MvaI CCWGG 1 cut(s) 236
MvnI CGCG 2 cut(s) 59, 392
MwoI GCNNNNNNNGC 4 cut(s) 130, 247, 362, 398
NarI GGCGCC 1 cut(s) 32
NcoI CCATGG 1 cut(s) 372
NdeII GATC 4 cut(s) 54, 80, 183, 196
NlaIII CATG 6 cut(s) 167, 280, 313, 322, 376, 516
NlaIV GGNNCC 1 cut(s) 33
NspI RCATGY 3 cut(s) 280, 313, 322
PaqCI CACCTGC 1 cut(s) 160
PciI ACATGT 2 cut(s) 309, 318
PflMI CCANNNNNTGG 2 cut(s) 145, 373
PkrI GCNGC 5 cut(s) 66, 135, 275, 355, 493
Ple19I CGATCG 1 cut(s) 83
PleI GAGTC 1 cut(s) 172
PluTI GGCGCC 1 cut(s) 35
PpsI GAGTC 1 cut(s) 172
PscI ACATGT 2 cut(s) 309, 318
Psp6I CCWGG 1 cut(s) 234
PspFI CCCAGC 1 cut(s) 124
PspGI CCWGG 1 cut(s) 234
PspN4I GGNNCC 1 cut(s) 33
PspPI GGNCC 4 cut(s) 43, 364, 477, 496
PstNI CAGNNNCTG 1 cut(s) 145
PvuI CGATCG 1 cut(s) 83
RsaI GTAC 1 cut(s) 287
RsaNI GTAC 1 cut(s) 286
SatI GCNGC 5 cut(s) 65, 134, 274, 354, 492
Sau3AI GATC 4 cut(s) 54, 80, 183, 196
Sau96I GGNCC 4 cut(s) 43, 364, 477, 496
SchI GAGTC 1 cut(s) 172
ScrFI CCNGG 1 cut(s) 236
SetI ASST 9 cut(s) 28, 42, 66, 126, 174, 258, 363, 384, 411
SfaNI GCATC 1 cut(s) 288
SfoI GGCGCC 1 cut(s) 33
SinI GGWCC 2 cut(s) 43, 496
SsiI CCGC 7 cut(s) 59, 273, 353, 356, 401, 491, 494
SspDI GGCGCC 1 cut(s) 31
StyD4I CCNGG 1 cut(s) 234
StyI CCWWGG 1 cut(s) 372
TaaI ACNGT 1 cut(s) 290
TaqI TCGA 2 cut(s) 324, 456
TaqII GACCGA 1 cut(s) 105
TauI GCSGC 3 cut(s) 276, 356, 494
TscAI CASTG 2 cut(s) 295, 355
TseI GCWGC 2 cut(s) 64, 133
TspGWI ACGGA 3 cut(s) 117, 273, 452
TspRI CASTG 2 cut(s) 295, 355
Van91I CCANNNNNTGG 2 cut(s) 145, 373
VpaK11BI GGWCC 2 cut(s) 43, 496
XceI RCATGY 3 cut(s) 280, 313, 322
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.