Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr01

Orthogroup ID

OG0017085

Physical Location & Seq

Genomic Coordinates Forward (+)

6474198 .. 6474686

Gene Structure 489 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD01G1013200.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 489 bp

ATGGAGGAGGAGCAGATGGCACAGCTGCGCAAGATCTTCCGATCGTTTGACGGCAACAAAGACGGCAGCTTGACCGAGCCGGAGTTGGGGTCACTGCTGCGGTCGCTTGGGCTGAAGCCGAGCCATGAGCAGCTGCAGGCTCTGATTCGAAAGTCGGATACCAACCACAACGGATTGGTCGGGTTCTCGGAGTTCGTGGCGCTGATGAGGCCGGACCTCAACGGACTCTTGCAAGACGACGCCAGGTGCCCGTGTTCGTACAGTGAGGATCAGTTGAGGGAGATGTTCAGCATGTTCGACAGGGACGCCAACGGGTACATCAGCCCGGCGGAACTGGCCCACTCGATGGCCAAGCTGGGCCACCCACTCACGGTTCAGGAGTTGTTCGGCATGATGAACGAGGCGGATACCGACCGCGATGGCCTTATCGATTTTCACGAGTTTTCCAGGGCCATCACCTCAGCCGCTTTCCTTAATATCAATGCTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000325 GO:0003674 GO:0005488 GO:0005509 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0008150 GO:0032879 GO:0043167 GO:0043169 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043269 GO:0044424 GO:0044444 GO:0044464 GO:0046872 GO:0050789 GO:0051049 GO:0065007

KEGG Pathways

Metabolic & Signaling

ko04626 map04626

Pfam Domains

Protein Families

EF-hand_1 EF-hand_5 EF-hand_7 EF-hand_8

Protein Analysis

163

Amino Acids

18.16

Weight (kDa)

4.77

Isoelectric Point (pI)

42.72

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
EF-hand_7	PF13499	7 - 70	2.7e-16	EF-hand domain pair
EF-hand_6	PF13405	8 - 37	1.3e-06	EF-hand domain
EF-hand_8	PF13833	21 - 69	2.1e-06	EF-hand domain pair
EF-hand_7	PF13499	89 - 152	1.1e-14	EF-hand domain pair
EF-hand_6	PF13405	91 - 120	2.9e-06	EF-hand domain
EF-hand_1	PF00036	92 - 119	8.2e-07	EF hand domain
EF-hand_8	PF13833	104 - 150	1.7e-09	EF-hand domain pair
EF-hand_1	PF00036	128 - 154	2.6e-06	EF hand domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

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Tree File

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Publication-ready

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Orthologous Genes (Group: OG0017085)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G03000
fragaria_vesca	FvH4_7g34050
malus_domestica	MD01G1013200.v1.1
pyrus_communis	pycom01g04530
rosa_chinensis	RchiOBHm_Chr1g0384321
rosa_laevigata	RLG00000026049
rosa_multiflora	Rmu_co8218092.1_g000001 Rmu_sc0016737.1_g000003 Rmu_sc0018832.1_g000002
rosa_roxburghii	Rroxscaffold_4G00276780
rosa_samantha	Rh1BG430000 Rh1DG462600
rosa_wichuraiana	Rw1G040040

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc16I	TGCGCA	1 cut(s)	29
AccB1I	GGYRCC	1 cut(s)	246
AccB7I	CCANNNNNTGG	1 cut(s)	346
AccII	CGCG	1 cut(s)	417
AciI	CCGC	5 cut(s)	100, 329, 404, 415, 465
AclWI	GGATC	1 cut(s)	276
AcoI	YGGCCR	1 cut(s)	348
AcuI	CTGAAG	1 cut(s)	134
AcyI	GRCGYC	2 cut(s)	240, 306
AfaI	GTAC	2 cut(s)	260, 317
AfiI	CCNNNNNNNGG	3 cut(s)	86, 346, 370
AjnI	CCWGG	2 cut(s)	242, 446
AluBI	AGCT	4 cut(s)	25, 69, 133, 355
AluI	AGCT	4 cut(s)	25, 69, 133, 355
AlwI	GGATC	1 cut(s)	276
AlwNI	CAGNNNCTG	1 cut(s)	142
AoxI	GGCC	6 cut(s)	209, 336, 348, 358, 421, 450
ApeKI	GCWGC	5 cut(s)	25, 66, 97, 130, 133
AspLEI	GCGC	2 cut(s)	30, 202
AspS9I	GGNCC	4 cut(s)	214, 337, 358, 450
AsuC2I	CCSGG	1 cut(s)	326
AsuHPI	GGTGA	1 cut(s)	448
AsuII	TTCGAA	1 cut(s)	148
AvaII	GGWCC	1 cut(s)	214
BaeGI	GKGCMC	1 cut(s)	251
BalI	TGGCCA	1 cut(s)	350
BanI	GGYRCC	1 cut(s)	246
BauI	CACGAG	1 cut(s)	437
BbvCI	CCTCAGC	1 cut(s)	460
BbvI	GCAGC	5 cut(s)	12, 78, 84, 120, 142
BccI	CCATC	4 cut(s)	10, 340, 413, 461
BceAI	ACGGC	2 cut(s)	67, 79
BciT130I	CCWGG	2 cut(s)	244, 448
BciVI	GTATCC	2 cut(s)	151, 400
BcnI	CCSGG	1 cut(s)	326
BfmI	CTRYAG	1 cut(s)	134
BfoI	RGCGCY	1 cut(s)	203
BfuI	GTATCC	2 cut(s)	151, 400
BglII	AGATCT	1 cut(s)	33
BisI	GCNGC	6 cut(s)	26, 67, 98, 131, 134, 465
BlsI	GCNGC	6 cut(s)	27, 68, 99, 132, 135, 466
Bme1390I	CCNGG	3 cut(s)	244, 326, 448
Bme18I	GGWCC	1 cut(s)	214
BmgT120I	GGNCC	4 cut(s)	214, 337, 358, 450
BmiI	GGNNCC	1 cut(s)	248
BmrFI	CCNGG	3 cut(s)	244, 326, 448
Bpu10I	CCTNAGC	1 cut(s)	460
Bpu14I	TTCGAA	1 cut(s)	148
BpuMI	CCSGG	1 cut(s)	326
Bsa29I	ATCGAT	1 cut(s)	429
BsaHI	GRCGYC	2 cut(s)	240, 306
BsaJI	CCNNGG	1 cut(s)	447
BsaXI	ACNNNNNCTCC	2 cut(s)	74, 104
Bsc4I	CCNNNNNNNGG	3 cut(s)	86, 346, 370
Bse1I	ACTGG	1 cut(s)	339
BseBI	CCWGG	2 cut(s)	244, 448
BseCI	ATCGAT	1 cut(s)	429
BseDI	CCNNGG	1 cut(s)	447
BseLI	CCNNNNNNNGG	3 cut(s)	86, 346, 370
BseMII	CTCAG	1 cut(s)	474
BseNI	ACTGG	1 cut(s)	339
BseRI	GAGGAG	2 cut(s)	20, 23
BseSI	GKGCMC	1 cut(s)	251
BseXI	GCAGC	5 cut(s)	12, 78, 84, 120, 142
BseYI	CCCAGC	1 cut(s)	355
Bsh1236I	CGCG	1 cut(s)	417
Bsh1285I	CGRYCG	3 cut(s)	44, 104, 415
BshFI	GGCC	6 cut(s)	211, 338, 350, 360, 423, 452
BshNI	GGYRCC	1 cut(s)	246
BshVI	ATCGAT	1 cut(s)	429
BsiEI	CGRYCG	3 cut(s)	44, 104, 415
BsiSI	CCGG	3 cut(s)	80, 212, 326
BslFI	GGGAC	1 cut(s)	317
BslI	CCNNNNNNNGG	3 cut(s)	86, 346, 370
BsmFI	GGGAC	1 cut(s)	317
BsnI	GGCC	6 cut(s)	211, 338, 350, 360, 423, 452
Bsp119I	TTCGAA	1 cut(s)	148
Bsp1286I	GDGCHC	1 cut(s)	251
Bsp143I	GATC	3 cut(s)	33, 41, 268
BspACI	CCGC	5 cut(s)	100, 329, 404, 415, 465
BspANI	GGCC	6 cut(s)	211, 338, 350, 360, 423, 452
BspCNI	CTCAG	1 cut(s)	473
BspDI	ATCGAT	1 cut(s)	429
BspFNI	CGCG	1 cut(s)	417
BspLI	GGNNCC	1 cut(s)	248
BspMAI	CTGCAG	1 cut(s)	138
BspPI	GGATC	1 cut(s)	276
BspT104I	TTCGAA	1 cut(s)	148
BspT107I	GGYRCC	1 cut(s)	246
BsrI	ACTGG	1 cut(s)	339
BssECI	CCNNGG	1 cut(s)	447
BssMI	GATC	3 cut(s)	33, 41, 268
BssNI	GRCGYC	2 cut(s)	240, 306
BssSI	CACGAG	1 cut(s)	437
Bst2BI	CACGAG	1 cut(s)	437
Bst2UI	CCWGG	2 cut(s)	244, 448
Bst4CI	ACNGT	2 cut(s)	263, 373
BstACI	GRCGYC	2 cut(s)	240, 306
BstBI	TTCGAA	1 cut(s)	148
BstC8I	GCNNGC	1 cut(s)	138
BstDEI	CTNAG	1 cut(s)	460
BstFNI	CGCG	1 cut(s)	417
BstH2I	RGCGCY	1 cut(s)	203
BstHHI	GCGC	2 cut(s)	30, 202
BstKTI	GATC	3 cut(s)	36, 44, 271
BstMBI	GATC	3 cut(s)	33, 41, 268
BstMCI	CGRYCG	3 cut(s)	44, 104, 415
BstMWI	GCNNNNNNNGC	3 cut(s)	103, 208, 335
BstNI	CCWGG	2 cut(s)	244, 448
BstNSI	RCATGY	1 cut(s)	295
BstSCI	CCNGG	3 cut(s)	242, 324, 446
BstSFI	CTRYAG	1 cut(s)	134
BstSLI	GKGCMC	1 cut(s)	251
BstUI	CGCG	1 cut(s)	417
BstV1I	GCAGC	5 cut(s)	12, 78, 84, 120, 142
BstX2I	RGATCY	1 cut(s)	33
BstYI	RGATCY	1 cut(s)	33
Bsu15I	ATCGAT	1 cut(s)	429
BsuI	GTATCC	2 cut(s)	151, 400
BsuRI	GGCC	6 cut(s)	211, 338, 350, 360, 423, 452
BsuTUI	ATCGAT	1 cut(s)	429
BtgZI	GCGATG	1 cut(s)	432
BtsI	GCAGTG	1 cut(s)	92
BtsIMutI	CAGTG	2 cut(s)	92, 268
Cac8I	GCNNGC	1 cut(s)	138
CaiI	CAGNNNCTG	1 cut(s)	142
CfoI	GCGC	2 cut(s)	30, 202
Cfr13I	GGNCC	4 cut(s)	214, 337, 358, 450
ClaI	ATCGAT	1 cut(s)	429
CseI	GACGC	2 cut(s)	248, 314
Csp6I	GTAC	2 cut(s)	259, 316
CviAII	CATG	3 cut(s)	125, 292, 391
CviQI	GTAC	2 cut(s)	259, 316
DdeI	CTNAG	1 cut(s)	460
DpnI	GATC	3 cut(s)	35, 43, 270
DpnII	GATC	3 cut(s)	33, 41, 268
EaeI	YGGCCR	1 cut(s)	348
EciI	GGCGGA	2 cut(s)	344, 419
Eco47I	GGWCC	1 cut(s)	214
Eco57I	CTGAAG	1 cut(s)	134
EcoRII	CCWGG	2 cut(s)	242, 446
FaeI	CATG	3 cut(s)	128, 295, 394
FaiI	YATR	3 cut(s)	126, 293, 392
FaqI	GGGAC	1 cut(s)	317
FatI	CATG	3 cut(s)	124, 291, 390
Fnu4HI	GCNGC	6 cut(s)	26, 67, 98, 131, 134, 465
Fsp4HI	GCNGC	6 cut(s)	26, 67, 98, 131, 134, 465
FspI	TGCGCA	1 cut(s)	29
GlaI	GCGC	2 cut(s)	29, 201
GluI	GCNGC	6 cut(s)	26, 67, 98, 131, 134, 465
GsaI	CCCAGC	1 cut(s)	359
HaeII	RGCGCY	1 cut(s)	203
HaeIII	GGCC	6 cut(s)	211, 338, 350, 360, 423, 452
HapII	CCGG	3 cut(s)	80, 212, 326
HgaI	GACGC	2 cut(s)	248, 314
HhaI	GCGC	2 cut(s)	30, 202
Hin1I	GRCGYC	2 cut(s)	240, 306
Hin1II	CATG	3 cut(s)	128, 295, 394
Hin6I	GCGC	2 cut(s)	28, 200
HinP1I	GCGC	2 cut(s)	28, 200
HinfI	GANTC	2 cut(s)	145, 225
HpaII	CCGG	3 cut(s)	80, 212, 326
HphI	GGTGA	1 cut(s)	448
Hpy188I	TCNGA	4 cut(s)	41, 144, 157, 190
Hpy188III	TCNNGA	2 cut(s)	377, 437
Hpy99I	CGWCG	1 cut(s)	242
HpyCH4III	ACNGT	2 cut(s)	263, 373
HpyCH4V	TGCA	2 cut(s)	136, 232
HpyF10VI	GCNNNNNNNGC	3 cut(s)	103, 208, 335
HpyF3I	CTNAG	1 cut(s)	460
Hsp92I	GRCGYC	2 cut(s)	240, 306
Hsp92II	CATG	3 cut(s)	128, 295, 394
HspAI	GCGC	2 cut(s)	28, 200
Kzo9I	GATC	3 cut(s)	33, 41, 268
LmnI	GCTCC	1 cut(s)	10
Lsp1109I	GCAGC	5 cut(s)	12, 78, 84, 120, 142
MaeIII	GTNAC	1 cut(s)	90
MalI	GATC	3 cut(s)	35, 43, 270
MboI	GATC	3 cut(s)	33, 41, 268
MboII	GAAGA	1 cut(s)	28
MflI	RGATCY	1 cut(s)	33
MhlI	GDGCHC	1 cut(s)	251
MlsI	TGGCCA	1 cut(s)	350
MluNI	TGGCCA	1 cut(s)	350
MlyI	GAGTC	1 cut(s)	219
MmeI	TCCRAC	1 cut(s)	135
MnlI	CCTC	6 cut(s)	201, 227, 259, 270, 394, 469
Mox20I	TGGCCA	1 cut(s)	350
MscI	TGGCCA	1 cut(s)	350
MseI	TTAA	2 cut(s)	474, 487
Msp20I	TGGCCA	1 cut(s)	350
MspA1I	CMGCKG	2 cut(s)	25, 133
MspI	CCGG	3 cut(s)	80, 212, 326
MspR9I	CCNGG	3 cut(s)	244, 326, 448
MvaI	CCWGG	2 cut(s)	244, 448
MvnI	CGCG	1 cut(s)	417
MwoI	GCNNNNNNNGC	3 cut(s)	103, 208, 335
NciI	CCSGG	1 cut(s)	326
NdeII	GATC	3 cut(s)	33, 41, 268
NlaIII	CATG	3 cut(s)	128, 295, 394
NlaIV	GGNNCC	1 cut(s)	248
NmeAIII	GCCGAG	1 cut(s)	144
NmuCI	GTSAC	1 cut(s)	90
NsbI	TGCGCA	1 cut(s)	29
NspI	RCATGY	1 cut(s)	295
NspV	TTCGAA	1 cut(s)	148
PcsI	WCGNNNNNNNCGW	1 cut(s)	435
PfeI	GAWTC	1 cut(s)	145
PflMI	CCANNNNNTGG	1 cut(s)	346
PkrI	GCNGC	6 cut(s)	27, 68, 99, 132, 135, 466
Ple19I	CGATCG	1 cut(s)	44
PleI	GAGTC	1 cut(s)	219
PpsI	GAGTC	1 cut(s)	219
Psp6I	CCWGG	2 cut(s)	242, 446
PspFI	CCCAGC	1 cut(s)	355
PspGI	CCWGG	2 cut(s)	242, 446
PspN4I	GGNNCC	1 cut(s)	248
PspPI	GGNCC	4 cut(s)	214, 337, 358, 450
PstI	CTGCAG	1 cut(s)	138
PstNI	CAGNNNCTG	1 cut(s)	142
PsuI	RGATCY	1 cut(s)	33
PvuI	CGATCG	1 cut(s)	44
PvuII	CAGCTG	2 cut(s)	25, 133
RsaI	GTAC	2 cut(s)	260, 317
RsaNI	GTAC	2 cut(s)	259, 316
SaqAI	TTAA	2 cut(s)	474, 487
SatI	GCNGC	6 cut(s)	26, 67, 98, 131, 134, 465
Sau3AI	GATC	3 cut(s)	33, 41, 268
Sau96I	GGNCC	4 cut(s)	214, 337, 358, 450
SchI	GAGTC	1 cut(s)	219
ScrFI	CCNGG	3 cut(s)	244, 326, 448
SduI	GDGCHC	1 cut(s)	251
SetI	ASST	7 cut(s)	27, 71, 135, 219, 248, 357, 461
SfcI	CTRYAG	1 cut(s)	134
SfuI	TTCGAA	1 cut(s)	148
SinI	GGWCC	1 cut(s)	214
SsiI	CCGC	5 cut(s)	100, 329, 404, 415, 465
StyD4I	CCNGG	3 cut(s)	242, 324, 446
TaaI	ACNGT	2 cut(s)	263, 373
TaqI	TCGA	4 cut(s)	148, 297, 344, 429
TaqII	GACCGA	1 cut(s)	89
TauI	GCSGC	1 cut(s)	467
TfiI	GAWTC	1 cut(s)	145
Tru1I	TTAA	2 cut(s)	474, 487
Tru9I	TTAA	2 cut(s)	474, 487
TscAI	CASTG	2 cut(s)	99, 268
TseFI	GTSAC	1 cut(s)	90
TseI	GCWGC	5 cut(s)	25, 66, 97, 130, 133
Tsp45I	GTSAC	1 cut(s)	90
TspDTI	ATGAA	1 cut(s)	410
TspGWI	ACGGA	2 cut(s)	186, 237
TspRI	CASTG	2 cut(s)	99, 268
Van91I	CCANNNNNTGG	1 cut(s)	346
VpaK11BI	GGWCC	1 cut(s)	214
XceI	RCATGY	1 cut(s)	295

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.