Rroxscaffold_4G00276780

calcium-binding protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0017085
Physical Location & Seq
Reverse (-)
261426 .. 264902
3477 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00276780.1

Sequence Viewer

Length: 672 bp
ATGGGCAAAGGGAAAATTGCTGCTCAATGCAGGTGGGAGATGTGTGCACAACCTAAAGTTGTTCTAAAGGTAGAAACTGAAGAAGAGATGCTTGTTTTGCAAGATAGAGCAAAATCACTTAACTTACCAAACCATATTACAATTGATGCTGGGAAGACTCAGATTGCACCAAAAAGCAATCAGCTCCGGCCGAAGCTGGACCGCGAGCAGATCACGGAGCTGCGAGAGATATTCCGATCGTTTGACCGAAACAACGACGGAAGTTTGACCCCTCTGGAGCTGGGGTCGCTGCTCCGGTCACTGGGGCTGAAGGCGAGCCATAAGCAGGTGGAGACTCTGATCCAAAAGGCTGATCGTAACTACAACGGGCTGGTGGAGTTCTCGGAGTTCCTGGTGCTGGTGAAGCCTGACCTACTACTACTGCAAGGCGGCATGTGTCCGTACAGCGAGGAGATGCTGAGGAACATGTTTGACATGTTCGATAGGGATGGTAATGGCTACATCAGTGCGGCGGAGCTGGCCCACTCCATGGCCAAGCTTGGCCATGCGCTCACGGCGGAGGAGCTGATTGGGATGATGGAGGAGGCCGATACGGATGGAGATGGCCAAATCGATTTTCAGGAGTTTGCAAGGGCCATTACATCGGCGGCGGTCCATAATAGTTGGACATGA

Protein Analysis

223

Amino Acids

24.98

Weight (kDa)

5.12

Isoelectric Point (pI)

37.86

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PTH2 PF01981 11 - 60 6.3e-12 Peptidyl-tRNA hydrolase PTH2
EF-hand_7 PF13499 72 - 131 8e-14 EF-hand domain pair
EF-hand_1 PF00036 73 - 101 4.9e-06 EF hand domain
EF-hand_6 PF13405 73 - 102 6.6e-08 EF-hand domain
EF-hand_7 PF13499 150 - 213 2.1e-16 EF-hand domain pair
EF-hand_1 PF00036 153 - 180 1.5e-07 EF hand domain
EF-hand_6 PF13405 153 - 181 3.6e-07 EF-hand domain
EF-hand_8 PF13833 166 - 211 1.4e-08 EF-hand domain pair
EF-hand_1 PF00036 189 - 215 1.1e-07 EF hand domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Orthologous Genes (Group: OG0017085)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G03000
fragaria_vesca FvH4_7g34050
malus_domestica MD01G1013200.v1.1
pyrus_communis pycom01g04530
rosa_chinensis RchiOBHm_Chr1g0384321
rosa_laevigata RLG00000026049
rosa_multiflora Rmu_co8218092.1_g000001 Rmu_sc0016737.1_g000003 Rmu_sc0018832.1_g000002
rosa_roxburghii Rroxscaffold_4G00276780
rosa_samantha Rh1BG430000 Rh1DG462600
rosa_wichuraiana Rw1G040040

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 2 cut(s) 21, 316
Acc36I ACCTGC 2 cut(s) 21, 316
AccB7I CCANNNNNTGG 1 cut(s) 529
AccII CGCG 1 cut(s) 204
AciI CCGC 7 cut(s) 202, 429, 509, 512, 557, 647, 650
AclWI GGATC 1 cut(s) 334
AcoI YGGCCR 4 cut(s) 188, 531, 541, 604
AcuI CTGAAG 2 cut(s) 99, 329
AfaI GTAC 1 cut(s) 443
AfiI CCNNNNNNNGG 4 cut(s) 301, 325, 397, 529
AflIII ACRYGT 2 cut(s) 465, 474
AjnI CCWGG 1 cut(s) 390
AjuI GAANNNNNNNTTGG 2 cut(s) 600, 632
AluBI AGCT 7 cut(s) 184, 196, 220, 280, 517, 538, 565
AluI AGCT 7 cut(s) 184, 196, 220, 280, 517, 538, 565
Alw21I GWGCWC 1 cut(s) 49
Alw26I GTCTC 1 cut(s) 326
Alw44I GTGCAC 1 cut(s) 45
AlwI GGATC 1 cut(s) 334
AoxI GGCC 7 cut(s) 188, 519, 531, 541, 585, 604, 633
ApaLI GTGCAC 1 cut(s) 45
ApeKI GCWGC 3 cut(s) 20, 220, 289
AspLEI GCGC 1 cut(s) 550
AspS9I GGNCC 4 cut(s) 199, 520, 633, 652
AsuHPI GGTGA 1 cut(s) 412
AvaII GGWCC 2 cut(s) 199, 652
BaeGI GKGCMC 1 cut(s) 49
BalI TGGCCA 3 cut(s) 533, 543, 606
BbsI GAAGAC 1 cut(s) 161
Bbv12I GWGCWC 1 cut(s) 49
BbvCI CCTCAGC 1 cut(s) 458
BbvI GCAGC 3 cut(s) 7, 207, 276
BccI CCATC 4 cut(s) 482, 571, 590, 596
BceAI ACGGC 1 cut(s) 570
BciT130I CCWGG 1 cut(s) 392
BcoDI GTCTC 1 cut(s) 326
BfuAI ACCTGC 2 cut(s) 21, 316
BisI GCNGC 6 cut(s) 21, 221, 290, 430, 510, 648
BlsI GCNGC 6 cut(s) 22, 222, 291, 431, 511, 649
Bme1390I CCNGG 1 cut(s) 392
Bme18I GGWCC 2 cut(s) 199, 652
BmgT120I GGNCC 4 cut(s) 199, 520, 633, 652
BmrFI CCNGG 1 cut(s) 392
BmrI ACTGGG 1 cut(s) 311
BmsI GCATC 3 cut(s) 78, 136, 444
BmuI ACTGGG 1 cut(s) 311
BpiI GAAGAC 1 cut(s) 161
BpmI CTGGAG 1 cut(s) 296
Bpu10I CCTNAGC 1 cut(s) 458
Bsa29I ATCGAT 1 cut(s) 612
BsaJI CCNNGG 1 cut(s) 528
BsaWI WCCGGW 1 cut(s) 294
BsaXI ACNNNNNCTCC 2 cut(s) 269, 299
Bsc4I CCNNNNNNNGG 4 cut(s) 301, 325, 397, 529
Bse1I ACTGG 1 cut(s) 306
BseBI CCWGG 1 cut(s) 392
BseCI ATCGAT 1 cut(s) 612
BseDI CCNNGG 1 cut(s) 528
BseGI GGATG 3 cut(s) 493, 579, 601
BseLI CCNNNNNNNGG 4 cut(s) 301, 325, 397, 529
BseMII CTCAG 2 cut(s) 173, 449
BseNI ACTGG 1 cut(s) 306
BseRI GAGGAG 3 cut(s) 464, 575, 596
BseSI GKGCMC 1 cut(s) 49
BseX3I CGGCCG 1 cut(s) 188
BseXI GCAGC 3 cut(s) 7, 207, 276
BseYI CCCAGC 2 cut(s) 149, 280
Bsh1236I CGCG 1 cut(s) 204
Bsh1285I CGRYCG 2 cut(s) 191, 239
BshFI GGCC 7 cut(s) 190, 521, 533, 543, 587, 606, 635
BshVI ATCGAT 1 cut(s) 612
BsiEI CGRYCG 2 cut(s) 191, 239
BsiHKAI GWGCWC 1 cut(s) 49
BsiSI CCGG 2 cut(s) 187, 295
BslI CCNNNNNNNGG 4 cut(s) 301, 325, 397, 529
BsmAI GTCTC 1 cut(s) 326
BsnI GGCC 7 cut(s) 190, 521, 533, 543, 587, 606, 635
Bsp1286I GDGCHC 1 cut(s) 49
Bsp143I GATC 4 cut(s) 210, 236, 339, 352
Bsp19I CCATGG 1 cut(s) 528
BspACI CCGC 7 cut(s) 202, 429, 509, 512, 557, 647, 650
BspANI GGCC 7 cut(s) 190, 521, 533, 543, 587, 606, 635
BspCNI CTCAG 2 cut(s) 172, 450
BspDI ATCGAT 1 cut(s) 612
BspFNI CGCG 1 cut(s) 204
BspMI ACCTGC 2 cut(s) 21, 316
BspPI GGATC 1 cut(s) 334
BsrI ACTGG 1 cut(s) 306
BssECI CCNNGG 1 cut(s) 528
BssMI GATC 4 cut(s) 210, 236, 339, 352
BssT1I CCWWGG 1 cut(s) 528
Bst2UI CCWGG 1 cut(s) 392
Bst6I CTCTTC 1 cut(s) 78
BstC8I GCNNGC 3 cut(s) 206, 316, 519
BstDEI CTNAG 2 cut(s) 159, 458
BstDSI CCRYGG 1 cut(s) 528
BstF5I GGATG 3 cut(s) 493, 579, 601
BstFNI CGCG 1 cut(s) 204
BstHHI GCGC 1 cut(s) 550
BstKTI GATC 4 cut(s) 213, 239, 342, 355
BstMAI GTCTC 1 cut(s) 326
BstMBI GATC 4 cut(s) 210, 236, 339, 352
BstMCI CGRYCG 2 cut(s) 191, 239
BstMWI GCNNNNNNNGC 5 cut(s) 97, 286, 403, 518, 554
BstNI CCWGG 1 cut(s) 392
BstNSI RCATGY 3 cut(s) 436, 469, 478
BstSCI CCNGG 1 cut(s) 390
BstSLI GKGCMC 1 cut(s) 49
BstUI CGCG 1 cut(s) 204
BstV1I GCAGC 3 cut(s) 7, 207, 276
BstV2I GAAGAC 1 cut(s) 161
BstZI CGGCCG 1 cut(s) 188
Bsu15I ATCGAT 1 cut(s) 612
BsuRI GGCC 7 cut(s) 190, 521, 533, 543, 587, 606, 635
BsuTUI ATCGAT 1 cut(s) 612
BtgI CCRYGG 1 cut(s) 528
BtsCI GGATG 3 cut(s) 493, 579, 601
BtsIMutI CAGTG 2 cut(s) 299, 511
BveI ACCTGC 2 cut(s) 21, 316
Cac8I GCNNGC 3 cut(s) 206, 316, 519
CfoI GCGC 1 cut(s) 550
Cfr13I GGNCC 4 cut(s) 199, 520, 633, 652
ClaI ATCGAT 1 cut(s) 612
Csp6I GTAC 1 cut(s) 442
CspCI CAANNNNNGTGG 2 cut(s) 14, 49
CviAII CATG 6 cut(s) 433, 466, 475, 529, 545, 669
CviQI GTAC 1 cut(s) 442
DdeI CTNAG 2 cut(s) 159, 458
DpnI GATC 4 cut(s) 212, 238, 341, 354
DpnII GATC 4 cut(s) 210, 236, 339, 352
EaeI YGGCCR 4 cut(s) 188, 531, 541, 604
EagI CGGCCG 1 cut(s) 188
Eam1104I CTCTTC 1 cut(s) 78
EarI CTCTTC 1 cut(s) 78
EciI GGCGGA 2 cut(s) 527, 572
EclXI CGGCCG 1 cut(s) 188
Eco130I CCWWGG 1 cut(s) 528
Eco47I GGWCC 2 cut(s) 199, 652
Eco52I CGGCCG 1 cut(s) 188
Eco57I CTGAAG 2 cut(s) 99, 329
EcoRII CCWGG 1 cut(s) 390
EcoT14I CCWWGG 1 cut(s) 528
ErhI CCWWGG 1 cut(s) 528
FaeI CATG 6 cut(s) 436, 469, 478, 532, 548, 672
FaiI YATR 9 cut(s) 135, 321, 434, 467, 476, 530, 546, 657, 670
FalI AAGNNNNNCTT 2 cut(s) 75, 107
FatI CATG 6 cut(s) 432, 465, 474, 528, 544, 668
Fnu4HI GCNGC 6 cut(s) 21, 221, 290, 430, 510, 648
FokI GGATG 3 cut(s) 500, 586, 608
Fsp4HI GCNGC 6 cut(s) 21, 221, 290, 430, 510, 648
GlaI GCGC 1 cut(s) 549
GluI GCNGC 6 cut(s) 21, 221, 290, 430, 510, 648
GsaI CCCAGC 2 cut(s) 153, 284
GsuI CTGGAG 1 cut(s) 296
HaeIII GGCC 7 cut(s) 190, 521, 533, 543, 587, 606, 635
HapII CCGG 2 cut(s) 187, 295
HhaI GCGC 1 cut(s) 550
Hin1II CATG 6 cut(s) 436, 469, 478, 532, 548, 672
Hin6I GCGC 1 cut(s) 548
HinP1I GCGC 1 cut(s) 548
HindIII AAGCTT 1 cut(s) 536
HinfI GANTC 2 cut(s) 157, 334
HpaII CCGG 2 cut(s) 187, 295
HphI GGTGA 1 cut(s) 412
Hpy166II GTNNAC 1 cut(s) 47
Hpy188I TCNGA 4 cut(s) 162, 236, 339, 385
Hpy188III TCNNGA 2 cut(s) 275, 620
Hpy8I GTNNAC 1 cut(s) 47
Hpy99I CGWCG 1 cut(s) 260
HpyAV CCTTC 1 cut(s) 304
HpyCH4V TGCA 6 cut(s) 30, 47, 100, 167, 424, 629
HpyF10VI GCNNNNNNNGC 5 cut(s) 97, 286, 403, 518, 554
HpyF3I CTNAG 2 cut(s) 159, 458
Hsp92II CATG 6 cut(s) 436, 469, 478, 532, 548, 672
HspAI GCGC 1 cut(s) 548
Kzo9I GATC 4 cut(s) 210, 236, 339, 352
LmnI GCTCC 6 cut(s) 189, 217, 277, 297, 514, 562
Lsp1109I GCAGC 3 cut(s) 7, 207, 276
LweI GCATC 3 cut(s) 78, 136, 444
MaeIII GTNAC 2 cut(s) 297, 356
MalI GATC 4 cut(s) 212, 238, 341, 354
MboI GATC 4 cut(s) 210, 236, 339, 352
MboII GAAGA 3 cut(s) 92, 95, 166
MfeI CAATTG 1 cut(s) 141
MhlI GDGCHC 1 cut(s) 49
MlsI TGGCCA 3 cut(s) 533, 543, 606
MluCI AATT 2 cut(s) 15, 141
MluNI TGGCCA 3 cut(s) 533, 543, 606
MlyI GAGTC 2 cut(s) 151, 328
MmeI TCCRAC 1 cut(s) 644
MnlI CCTC 6 cut(s) 282, 442, 453, 553, 574, 577
Mox20I TGGCCA 3 cut(s) 533, 543, 606
MscI TGGCCA 3 cut(s) 533, 543, 606
MseI TTAA 1 cut(s) 120
Msp20I TGGCCA 3 cut(s) 533, 543, 606
MspI CCGG 2 cut(s) 187, 295
MspR9I CCNGG 1 cut(s) 392
MunI CAATTG 1 cut(s) 141
MvaI CCWGG 1 cut(s) 392
MvnI CGCG 1 cut(s) 204
MwoI GCNNNNNNNGC 5 cut(s) 97, 286, 403, 518, 554
NcoI CCATGG 1 cut(s) 528
NdeII GATC 4 cut(s) 210, 236, 339, 352
NlaIII CATG 6 cut(s) 436, 469, 478, 532, 548, 672
NmuCI GTSAC 1 cut(s) 297
NspI RCATGY 3 cut(s) 436, 469, 478
PaqCI CACCTGC 2 cut(s) 21, 316
PciI ACATGT 2 cut(s) 465, 474
PflMI CCANNNNNTGG 1 cut(s) 529
PkrI GCNGC 6 cut(s) 22, 222, 291, 431, 511, 649
Ple19I CGATCG 1 cut(s) 239
PleI GAGTC 2 cut(s) 151, 328
PpsI GAGTC 2 cut(s) 151, 328
PscI ACATGT 2 cut(s) 465, 474
Psp6I CCWGG 1 cut(s) 390
PspFI CCCAGC 2 cut(s) 149, 280
PspGI CCWGG 1 cut(s) 390
PspPI GGNCC 4 cut(s) 199, 520, 633, 652
PvuI CGATCG 1 cut(s) 239
RsaI GTAC 1 cut(s) 443
RsaNI GTAC 1 cut(s) 442
SaqAI TTAA 1 cut(s) 120
SatI GCNGC 6 cut(s) 21, 221, 290, 430, 510, 648
Sau3AI GATC 4 cut(s) 210, 236, 339, 352
Sau96I GGNCC 4 cut(s) 199, 520, 633, 652
SchI GAGTC 2 cut(s) 151, 328
ScrFI CCNGG 1 cut(s) 392
SduI GDGCHC 1 cut(s) 49
SfaNI GCATC 3 cut(s) 78, 136, 444
SinI GGWCC 2 cut(s) 199, 652
Sse9I AATT 2 cut(s) 15, 141
SsiI CCGC 7 cut(s) 202, 429, 509, 512, 557, 647, 650
StyD4I CCNGG 1 cut(s) 390
StyI CCWWGG 1 cut(s) 528
TaqI TCGA 2 cut(s) 480, 612
TaqII GACCGA 1 cut(s) 261
TasI AATT 2 cut(s) 15, 141
TauI GCSGC 3 cut(s) 432, 512, 650
Tru1I TTAA 1 cut(s) 120
Tru9I TTAA 1 cut(s) 120
TscAI CASTG 2 cut(s) 306, 511
TseFI GTSAC 1 cut(s) 297
TseI GCWGC 3 cut(s) 20, 220, 289
Tsp45I GTSAC 1 cut(s) 297
TspGWI ACGGA 4 cut(s) 230, 273, 429, 608
TspRI CASTG 2 cut(s) 306, 511
Van91I CCANNNNNTGG 1 cut(s) 529
VneI GTGCAC 1 cut(s) 45
VpaK11BI GGWCC 2 cut(s) 199, 652
XceI RCATGY 3 cut(s) 436, 469, 478
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.