AT3G03100

Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
3
OG0013227
Physical Location & Seq
Reverse (-)
705381 .. 707697
2317 bp
Loading structure...
UTR
Exon/CDS
Intron
AT3G03100.2

Sequence Viewer

Length: 480 bp
ATGGCTTTGACGGTGGCGAAGAGTGCGTTGGAGGCGATTAGAGAGAAAGGTCTCGGCGGTTTCATGAGGATGATCCGAGAAGAAGGCTTCATGAGATGCCTACCAGATGGGAACCTCTTGCAAACTAAAATACATAACATAGGAGCGACACTTGTTGGTGTTGACAAGTTCGGTAACAAATACTACCAGAAGCTAGGCGATACTCAATACGGTAGGCACAGATGGGTAGAGTATGCTTCAAAGGATCGTTACAACGCATCTCAAGTACCAGCTGAATGGCACGGGTGGCTTCATTTCATCACTGATCACACTGGCGATGAGCTCTTGAGTTTGAAACCAAAAAGGTATGGGCTTGAGCATAAAGAGAACTTCTCTGGAGAAGGTGATGCATACATCTACCATTCGAAGGGACATACCTTGAACCCGGGACAAAAGAACTGGACTCGGTACCAATCATGGGTACCCACCAAGACTCAGTAA

Protein Analysis

159

Amino Acids

18.32

Weight (kDa)

9.25

Isoelectric Point (pI)

24.12

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NDUFA12 PF05071 50 - 155 1.3e-24 NADH ubiquinone oxidoreductase subunit NDUFA12
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0013227)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G03100 AT3G03100
fragaria_vesca FvH4_7g33890
malus_domestica MD01G1235400.v1.1 MD07G1308200.v1.1
prunus_persica Prupe.2G325400_v2.0.a1 Prupe.2G325400_v2.0.a1
pyrus_communis pycom07g28040
rosa_chinensis RchiOBHm_Chr1g0384091
rosa_laevigata RLG00000026073
rosa_multiflora Rmu_sc0005139.1_g000018
rosa_roxburghii Rroxscaffold_4G00277000
rosa_rugosa Rorug01G0444700
rosa_samantha Rh1AG470100 Rh1BG427600 Rh1CG440300 Rh1DG460600
rosa_wichuraiana Rw1G040250

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 2 cut(s) 447, 460
AccB1I GGYRCC 2 cut(s) 447, 460
AciI CCGC 1 cut(s) 57
AclWI GGATC 2 cut(s) 67, 252
AfaI GTAC 3 cut(s) 267, 449, 462
AfiI CCNNNNNNNGG 2 cut(s) 406, 457
AgsI TTSAA 3 cut(s) 240, 334, 421
AjuI GAANNNNNNNTTGG 2 cut(s) 11, 43
AluBI AGCT 3 cut(s) 193, 272, 322
AluI AGCT 3 cut(s) 193, 272, 322
Alw21I GWGCWC 1 cut(s) 324
Alw26I GTCTC 1 cut(s) 56
AlwI GGATC 2 cut(s) 67, 252
Ama87I CYCGRG 1 cut(s) 424
Asp718I GGTACC 2 cut(s) 447, 460
AsuC2I CCSGG 2 cut(s) 425, 426
AsuHPI GGTGA 1 cut(s) 395
AsuII TTCGAA 1 cut(s) 404
AvaI CYCGRG 1 cut(s) 424
BanI GGYRCC 2 cut(s) 447, 460
BanII GRGCYC 1 cut(s) 324
Bbv12I GWGCWC 1 cut(s) 324
BccI CCATC 2 cut(s) 101, 216
BclI TGATCA 1 cut(s) 304
BcnI CCSGG 2 cut(s) 425, 426
BcoDI GTCTC 1 cut(s) 56
BfaI CTAG 1 cut(s) 194
Bme1390I CCNGG 2 cut(s) 425, 426
BmeT110I CYCGRG 1 cut(s) 424
BmiI GGNNCC 3 cut(s) 113, 449, 462
BmrFI CCNGG 2 cut(s) 425, 426
BmsI GCATC 3 cut(s) 86, 266, 376
BplI GAGNNNNNCTC 2 cut(s) 356, 388
BpmI CTGGAG 1 cut(s) 396
Bpu14I TTCGAA 1 cut(s) 404
BpuEI CTTGAG 3 cut(s) 246, 346, 374
BpuMI CCSGG 2 cut(s) 425, 426
BsaI GGTCTC 1 cut(s) 56
BsaJI CCNNGG 1 cut(s) 424
Bsc4I CCNNNNNNNGG 2 cut(s) 406, 457
Bse1I ACTGG 2 cut(s) 316, 443
BseDI CCNNGG 1 cut(s) 424
BseGI GGATG 1 cut(s) 75
BseLI CCNNNNNNNGG 2 cut(s) 406, 457
BseNI ACTGG 2 cut(s) 316, 443
BshNI GGYRCC 2 cut(s) 447, 460
BsiHKAI GWGCWC 1 cut(s) 324
BsiHKCI CYCGRG 1 cut(s) 424
BsiSI CCGG 1 cut(s) 425
BslFI GGGAC 2 cut(s) 423, 441
BslI CCNNNNNNNGG 2 cut(s) 406, 457
BsmAI GTCTC 1 cut(s) 56
BsmFI GGGAC 2 cut(s) 423, 441
Bso31I GGTCTC 1 cut(s) 56
BsoBI CYCGRG 1 cut(s) 424
Bsp119I TTCGAA 1 cut(s) 404
Bsp1286I GDGCHC 1 cut(s) 324
Bsp143I GATC 3 cut(s) 72, 244, 304
BspACI CCGC 1 cut(s) 57
BspHI TCATGA 2 cut(s) 63, 90
BspLI GGNNCC 3 cut(s) 113, 449, 462
BspPI GGATC 2 cut(s) 67, 252
BspT104I TTCGAA 1 cut(s) 404
BspT107I GGYRCC 2 cut(s) 447, 460
BspTNI GGTCTC 1 cut(s) 56
BsrI ACTGG 2 cut(s) 316, 443
BssECI CCNNGG 1 cut(s) 424
BssMI GATC 3 cut(s) 72, 244, 304
Bst4CI ACNGT 2 cut(s) 13, 212
Bst6I CTCTTC 1 cut(s) 14
BstBI TTCGAA 1 cut(s) 404
BstDEI CTNAG 1 cut(s) 474
BstF5I GGATG 1 cut(s) 75
BstKTI GATC 3 cut(s) 75, 247, 307
BstMAI GTCTC 1 cut(s) 56
BstMBI GATC 3 cut(s) 72, 244, 304
BstMWI GCNNNNNNNGC 3 cut(s) 23, 32, 286
BstSCI CCNGG 2 cut(s) 423, 424
BstXI CCANNNNNNTGG 1 cut(s) 276
BtgZI GCGATG 1 cut(s) 330
BtsCI GGATG 1 cut(s) 75
BtsIMutI CAGTG 2 cut(s) 300, 309
CciI TCATGA 2 cut(s) 63, 90
Cfr9I CCCGGG 1 cut(s) 424
Csp6I GTAC 3 cut(s) 266, 448, 461
CviAII CATG 3 cut(s) 64, 91, 456
CviJI RGCY 7 cut(s) 5, 87, 193, 272, 289, 322, 352
CviKI_1 RGCY 7 cut(s) 5, 87, 193, 272, 289, 322, 352
CviQI GTAC 3 cut(s) 266, 448, 461
DdeI CTNAG 1 cut(s) 474
DpnI GATC 3 cut(s) 74, 246, 306
DpnII GATC 3 cut(s) 72, 244, 304
Eam1104I CTCTTC 1 cut(s) 14
EarI CTCTTC 1 cut(s) 14
Ecl136II GAGCTC 1 cut(s) 322
Eco24I GRGCYC 1 cut(s) 324
Eco31I GGTCTC 1 cut(s) 56
Eco53kI GAGCTC 1 cut(s) 322
Eco88I CYCGRG 1 cut(s) 424
EcoICRI GAGCTC 1 cut(s) 322
EcoT22I ATGCAT 1 cut(s) 391
EcoT38I GRGCYC 1 cut(s) 324
FaeI CATG 3 cut(s) 67, 94, 459
FaqI GGGAC 2 cut(s) 423, 441
FatI CATG 3 cut(s) 63, 90, 455
FbaI TGATCA 1 cut(s) 304
FokI GGATG 1 cut(s) 82
FriOI GRGCYC 1 cut(s) 324
FspBI CTAG 1 cut(s) 194
GsuI CTGGAG 1 cut(s) 396
HapII CCGG 1 cut(s) 425
Hin1II CATG 3 cut(s) 67, 94, 459
HincII GTYRAC 1 cut(s) 163
HindII GTYRAC 1 cut(s) 163
HinfI GANTC 2 cut(s) 442, 472
HpaII CCGG 1 cut(s) 425
HphI GGTGA 1 cut(s) 395
Hpy166II GTNNAC 1 cut(s) 163
Hpy188I TCNGA 1 cut(s) 77
Hpy188III TCNNGA 4 cut(s) 64, 91, 325, 375
Hpy8I GTNNAC 1 cut(s) 163
HpyAV CCTTC 3 cut(s) 77, 374, 400
HpyCH4III ACNGT 2 cut(s) 13, 212
HpyCH4V TGCA 2 cut(s) 121, 389
HpyF10VI GCNNNNNNNGC 3 cut(s) 23, 32, 286
HpyF3I CTNAG 1 cut(s) 474
Hsp92II CATG 3 cut(s) 67, 94, 459
KpnI GGTACC 2 cut(s) 451, 464
Ksp22I TGATCA 1 cut(s) 304
Kzo9I GATC 3 cut(s) 72, 244, 304
LmnI GCTCC 1 cut(s) 143
LpnPI CCDG 7 cut(s) 117, 200, 282, 297, 360, 424, 438
LweI GCATC 3 cut(s) 86, 266, 376
MaeI CTAG 1 cut(s) 194
MaeIII GTNAC 2 cut(s) 173, 248
MalI GATC 3 cut(s) 74, 246, 306
MboI GATC 3 cut(s) 72, 244, 304
MboII GAAGA 2 cut(s) 31, 92
MhlI GDGCHC 1 cut(s) 324
MlyI GAGTC 2 cut(s) 436, 466
MmeI TCCRAC 1 cut(s) 9
MnlI CCTC 3 cut(s) 25, 60, 125
Mph1103I ATGCAT 1 cut(s) 391
MslI CAYNNNNRTG 1 cut(s) 68
MspA1I CMGCKG 1 cut(s) 272
MspI CCGG 1 cut(s) 425
MspR9I CCNGG 2 cut(s) 425, 426
MwoI GCNNNNNNNGC 3 cut(s) 23, 32, 286
NciI CCSGG 2 cut(s) 425, 426
NdeII GATC 3 cut(s) 72, 244, 304
NlaIII CATG 3 cut(s) 67, 94, 459
NlaIV GGNNCC 3 cut(s) 113, 449, 462
NmeAIII GCCGAG 1 cut(s) 33
NsiI ATGCAT 1 cut(s) 391
NspV TTCGAA 1 cut(s) 404
PagI TCATGA 2 cut(s) 63, 90
PleI GAGTC 2 cut(s) 436, 466
PpsI GAGTC 2 cut(s) 436, 466
Psp124BI GAGCTC 1 cut(s) 324
PspN4I GGNNCC 3 cut(s) 113, 449, 462
PvuII CAGCTG 1 cut(s) 272
RsaI GTAC 3 cut(s) 267, 449, 462
RsaNI GTAC 3 cut(s) 266, 448, 461
RseI CAYNNNNRTG 1 cut(s) 68
SacI GAGCTC 1 cut(s) 324
Sau3AI GATC 3 cut(s) 72, 244, 304
SchI GAGTC 2 cut(s) 436, 466
ScrFI CCNGG 2 cut(s) 425, 426
SduI GDGCHC 1 cut(s) 324
SetI ASST 8 cut(s) 52, 117, 195, 274, 324, 347, 385, 419
SfaNI GCATC 3 cut(s) 86, 266, 376
SfuI TTCGAA 1 cut(s) 404
SmaI CCCGGG 1 cut(s) 426
SmiMI CAYNNNNRTG 1 cut(s) 68
SmlI CTYRAG 3 cut(s) 261, 325, 353
SmoI CTYRAG 3 cut(s) 261, 325, 353
SsiI CCGC 1 cut(s) 57
SspMI CTAG 1 cut(s) 194
SstI GAGCTC 1 cut(s) 324
StyD4I CCNGG 2 cut(s) 423, 424
TaaI ACNGT 2 cut(s) 13, 212
TaqI TCGA 1 cut(s) 404
TscAI CASTG 2 cut(s) 307, 316
TspDTI ATGAA 4 cut(s) 52, 79, 281, 286
TspMI CCCGGG 1 cut(s) 424
TspRI CASTG 2 cut(s) 307, 316
XmaI CCCGGG 1 cut(s) 424
XspI CTAG 1 cut(s) 194
Zsp2I ATGCAT 1 cut(s) 391
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.