Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr01

Orthogroup ID

OG0013227

Physical Location & Seq

Genomic Coordinates Forward (+)

32292205 .. 32294762

Gene Structure 2558 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD01G1235400.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 474 bp

ATGGCGACGGCGGTGAAGAGCGTTGCGAAGGCAATCAGAGAGAGAGGTCTCCGCAGCTACTTAAAGGAGCTCAAGGAGGATGGCTTCTTAAATTGTATTTTCGATGGAAACCTTACGCAAACTAAGATCCACAATATTGGGGCAAGGCTTGTAGGTGTTGATAACATTGGCAATAAATATTATGAGAAGCTTGACGCTCAATATGGAAGACACAGATGGGTTGAATATGCAGAGAAGAGTCGCTACAACGCTTCTCAAGTACCACCAGAATGGCATGGTTGGCTGCACTACATAACTGATCATACAGGAGATGAGCTTTTGATGCTAAAACCAAAGAGGTATGGCATTGAACACAAAGAAAATTTCACTGGGGAGGGTGATGAGTTTATTTACCATTCCAAGGGCCACAGCCTCAATCCAGGGCAGAGAGACTGGACCAGGTACCAACCTTGGGAACCAACCAAGAAGGAGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0005740 GO:0005743 GO:0005746 GO:0005747 GO:0006950 GO:0006979 GO:0008150 GO:0009719 GO:0009725 GO:0009735 GO:0010033 GO:0016020 GO:0019866 GO:0030964 GO:0031090 GO:0031966 GO:0031967 GO:0031975 GO:0032991 GO:0042221 GO:0043167 GO:0043169 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044425 GO:0044429 GO:0044444 GO:0044446 GO:0044455 GO:0044464 GO:0045271 GO:0046872 GO:0046914 GO:0050896 GO:0050897 GO:0070469 GO:0098796 GO:0098798 GO:0098800 GO:0098803 GO:1902494 GO:1990204

KEGG Pathways

Metabolic & Signaling

ko00190 ko01100 ko04714 ko04723 ko04932 ko05010 ko05012 ko05016 map00190 map01100 map04714 map04723 map04932 map05010 map05012 map05016

Pfam Domains

Protein Families

NDUFA12

Protein Analysis

158

Amino Acids

18.41

Weight (kDa)

8.53

Isoelectric Point (pI)

35.15

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
NDUFA12	PF05071	49 - 153	1.5e-26	NADH ubiquinone oxidoreductase subunit NDUFA12

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Publication-ready

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Orthologous Genes (Group: OG0013227)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G03100 AT3G03100
fragaria_vesca	FvH4_7g33890
malus_domestica	MD01G1235400.v1.1 MD07G1308200.v1.1
prunus_persica	Prupe.2G325400_v2.0.a1 Prupe.2G325400_v2.0.a1
pyrus_communis	pycom07g28040
rosa_chinensis	RchiOBHm_Chr1g0384091
rosa_laevigata	RLG00000026073
rosa_multiflora	Rmu_sc0005139.1_g000018
rosa_roxburghii	Rroxscaffold_4G00277000
rosa_rugosa	Rorug01G0444700
rosa_samantha	Rh1AG470100 Rh1BG427600 Rh1CG440300 Rh1DG460600
rosa_wichuraiana	Rw1G040250

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc65I	GGTACC	1 cut(s)	441
AccB1I	GGYRCC	1 cut(s)	441
AciI	CCGC	2 cut(s)	11, 52
AclWI	GGATC	1 cut(s)	121
AcsI	RAATTY	1 cut(s)	361
AfaI	GTAC	2 cut(s)	261, 443
AfiI	CCNNNNNNNGG	2 cut(s)	400, 451
AgsI	TTSAA	2 cut(s)	224, 350
AjnI	CCWGG	2 cut(s)	418, 437
AluBI	AGCT	4 cut(s)	57, 70, 190, 316
AluI	AGCT	4 cut(s)	57, 70, 190, 316
Alw21I	GWGCWC	1 cut(s)	72
Alw26I	GTCTC	2 cut(s)	53, 423
AlwI	GGATC	1 cut(s)	121
AoxI	GGCC	1 cut(s)	403
ApeKI	GCWGC	2 cut(s)	54, 283
ApoI	RAATTY	1 cut(s)	361
Asp718I	GGTACC	1 cut(s)	441
AspS9I	GGNCC	2 cut(s)	403, 435
AsuHPI	GGTGA	2 cut(s)	25, 389
AvaII	GGWCC	1 cut(s)	435
BanI	GGYRCC	1 cut(s)	441
BanII	GRGCYC	1 cut(s)	72
BarI	GAAGNNNNNNTAC	2 cut(s)	227, 259
BbsI	GAAGAC	1 cut(s)	214
Bbv12I	GWGCWC	1 cut(s)	72
BbvI	GCAGC	2 cut(s)	66, 270
BccI	CCATC	3 cut(s)	74, 98, 210
BceAI	ACGGC	1 cut(s)	24
BciT130I	CCWGG	2 cut(s)	420, 439
BclI	TGATCA	1 cut(s)	298
BcoDI	GTCTC	2 cut(s)	53, 423
BisI	GCNGC	2 cut(s)	55, 284
BlsI	GCNGC	2 cut(s)	56, 285
Bme1390I	CCNGG	2 cut(s)	420, 439
Bme18I	GGWCC	1 cut(s)	435
BmgT120I	GGNCC	2 cut(s)	403, 435
BmiI	GGNNCC	2 cut(s)	443, 456
BmrFI	CCNGG	2 cut(s)	420, 439
BmrI	ACTGGG	1 cut(s)	378
BmsI	GCATC	1 cut(s)	312
BmuI	ACTGGG	1 cut(s)	378
BpiI	GAAGAC	1 cut(s)	214
BpuEI	CTTGAG	2 cut(s)	56, 240
BsaI	GGTCTC	1 cut(s)	53
BsaJI	CCNNGG	3 cut(s)	399, 419, 449
Bsc4I	CCNNNNNNNGG	2 cut(s)	400, 451
Bse1I	ACTGG	2 cut(s)	373, 437
BseBI	CCWGG	2 cut(s)	420, 439
BseDI	CCNNGG	3 cut(s)	399, 419, 449
BseGI	GGATG	1 cut(s)	85
BseLI	CCNNNNNNNGG	2 cut(s)	400, 451
BseNI	ACTGG	2 cut(s)	373, 437
BseXI	GCAGC	2 cut(s)	66, 270
BsgI	GTGCAG	1 cut(s)	269
BshFI	GGCC	1 cut(s)	405
BshNI	GGYRCC	1 cut(s)	441
BsiHKAI	GWGCWC	1 cut(s)	72
BslI	CCNNNNNNNGG	2 cut(s)	400, 451
BsmAI	GTCTC	2 cut(s)	53, 423
BsnI	GGCC	1 cut(s)	405
Bso31I	GGTCTC	1 cut(s)	53
Bsp1286I	GDGCHC	1 cut(s)	72
Bsp143I	GATC	2 cut(s)	126, 298
BspACI	CCGC	2 cut(s)	11, 52
BspANI	GGCC	1 cut(s)	405
BspLI	GGNNCC	2 cut(s)	443, 456
BspPI	GGATC	1 cut(s)	121
BspQI	GCTCTTC	1 cut(s)	11
BspT107I	GGYRCC	1 cut(s)	441
BspTNI	GGTCTC	1 cut(s)	53
BsrI	ACTGG	2 cut(s)	373, 437
BssECI	CCNNGG	3 cut(s)	399, 419, 449
BssMI	GATC	2 cut(s)	126, 298
BssT1I	CCWWGG	2 cut(s)	399, 449
Bst2UI	CCWGG	2 cut(s)	420, 439
Bst6I	CTCTTC	2 cut(s)	11, 230
BstDEI	CTNAG	1 cut(s)	123
BstF5I	GGATG	1 cut(s)	85
BstKTI	GATC	2 cut(s)	129, 301
BstMAI	GTCTC	2 cut(s)	53, 423
BstMBI	GATC	2 cut(s)	126, 298
BstMWI	GCNNNNNNNGC	2 cut(s)	280, 322
BstNI	CCWGG	2 cut(s)	420, 439
BstSCI	CCNGG	2 cut(s)	418, 437
BstV1I	GCAGC	2 cut(s)	66, 270
BstV2I	GAAGAC	1 cut(s)	214
BstX2I	RGATCY	1 cut(s)	126
BstXI	CCANNNNNNTGG	2 cut(s)	137, 270
BstYI	RGATCY	1 cut(s)	126
BsuRI	GGCC	1 cut(s)	405
BtsCI	GGATG	1 cut(s)	85
BtsIMutI	CAGTG	1 cut(s)	366
Cfr13I	GGNCC	2 cut(s)	403, 435
CseI	GACGC	1 cut(s)	203
CsiI	ACCWGGT	1 cut(s)	437
Csp6I	GTAC	2 cut(s)	260, 442
CviAII	CATG	1 cut(s)	275
CviJI	RGCY	9 cut(s)	57, 70, 84, 148, 190, 283, 316, 405, 411
CviKI_1	RGCY	9 cut(s)	57, 70, 84, 148, 190, 283, 316, 405, 411
CviQI	GTAC	2 cut(s)	260, 442
DdeI	CTNAG	1 cut(s)	123
DpnI	GATC	2 cut(s)	128, 300
DpnII	GATC	2 cut(s)	126, 298
Eam1104I	CTCTTC	2 cut(s)	11, 230
EarI	CTCTTC	2 cut(s)	11, 230
Ecl136II	GAGCTC	1 cut(s)	70
Eco130I	CCWWGG	2 cut(s)	399, 449
Eco24I	GRGCYC	1 cut(s)	72
Eco31I	GGTCTC	1 cut(s)	53
Eco47I	GGWCC	1 cut(s)	435
Eco53kI	GAGCTC	1 cut(s)	70
EcoICRI	GAGCTC	1 cut(s)	70
EcoRII	CCWGG	2 cut(s)	418, 437
EcoT14I	CCWWGG	2 cut(s)	399, 449
EcoT38I	GRGCYC	1 cut(s)	72
ErhI	CCWWGG	2 cut(s)	399, 449
FaeI	CATG	1 cut(s)	278
FaiI	YATR	7 cut(s)	183, 204, 228, 276, 293, 303, 342
FatI	CATG	1 cut(s)	274
FbaI	TGATCA	1 cut(s)	298
Fnu4HI	GCNGC	2 cut(s)	55, 284
FokI	GGATG	1 cut(s)	92
FriOI	GRGCYC	1 cut(s)	72
Fsp4HI	GCNGC	2 cut(s)	55, 284
GluI	GCNGC	2 cut(s)	55, 284
HaeIII	GGCC	1 cut(s)	405
HgaI	GACGC	1 cut(s)	203
Hin1II	CATG	1 cut(s)	278
HindIII	AAGCTT	1 cut(s)	188
HinfI	GANTC	1 cut(s)	238
HphI	GGTGA	2 cut(s)	25, 389
Hpy188I	TCNGA	1 cut(s)	38
Hpy99I	CGWCG	1 cut(s)	10
HpyAV	CCTTC	2 cut(s)	22, 460
HpyCH4V	TGCA	2 cut(s)	230, 286
HpyF10VI	GCNNNNNNNGC	2 cut(s)	280, 322
HpyF3I	CTNAG	1 cut(s)	123
Hsp92II	CATG	1 cut(s)	278
KpnI	GGTACC	1 cut(s)	445
Ksp22I	TGATCA	1 cut(s)	298
Kzo9I	GATC	2 cut(s)	126, 298
LguI	GCTCTTC	1 cut(s)	11
LmnI	GCTCC	1 cut(s)	67
LpnPI	CCDG	8 cut(s)	279, 291, 354, 405, 418, 424, 432, 451
Lsp1109I	GCAGC	2 cut(s)	66, 270
LweI	GCATC	1 cut(s)	312
MabI	ACCWGGT	1 cut(s)	437
MalI	GATC	2 cut(s)	128, 300
MboI	GATC	2 cut(s)	126, 298
MboII	GAAGA	3 cut(s)	28, 219, 247
MflI	RGATCY	1 cut(s)	126
MhlI	GDGCHC	1 cut(s)	72
MluCI	AATT	2 cut(s)	91, 361
MlyI	GAGTC	1 cut(s)	247
MnlI	CCTC	5 cut(s)	38, 70, 330, 367, 422
MseI	TTAA	2 cut(s)	62, 89
MslI	CAYNNNNRTG	1 cut(s)	268
MspR9I	CCNGG	2 cut(s)	420, 439
MvaI	CCWGG	2 cut(s)	420, 439
MwoI	GCNNNNNNNGC	2 cut(s)	280, 322
NdeII	GATC	2 cut(s)	126, 298
NlaIII	CATG	1 cut(s)	278
NlaIV	GGNNCC	2 cut(s)	443, 456
PciSI	GCTCTTC	1 cut(s)	11
PkrI	GCNGC	2 cut(s)	56, 285
PleI	GAGTC	1 cut(s)	246
PpsI	GAGTC	1 cut(s)	246
Psp124BI	GAGCTC	1 cut(s)	72
Psp6I	CCWGG	2 cut(s)	418, 437
PspGI	CCWGG	2 cut(s)	418, 437
PspN4I	GGNNCC	2 cut(s)	443, 456
PspPI	GGNCC	2 cut(s)	403, 435
PsuI	RGATCY	1 cut(s)	126
RsaI	GTAC	2 cut(s)	261, 443
RsaNI	GTAC	2 cut(s)	260, 442
RseI	CAYNNNNRTG	1 cut(s)	268
SacI	GAGCTC	1 cut(s)	72
SapI	GCTCTTC	1 cut(s)	11
SaqAI	TTAA	2 cut(s)	62, 89
SatI	GCNGC	2 cut(s)	55, 284
Sau3AI	GATC	2 cut(s)	126, 298
Sau96I	GGNCC	2 cut(s)	403, 435
SchI	GAGTC	1 cut(s)	247
ScrFI	CCNGG	2 cut(s)	420, 439
SduI	GDGCHC	1 cut(s)	72
SexAI	ACCWGGT	1 cut(s)	437
SfaNI	GCATC	1 cut(s)	312
SinI	GGWCC	1 cut(s)	435
SmiMI	CAYNNNNRTG	1 cut(s)	268
SmlI	CTYRAG	2 cut(s)	71, 255
SmoI	CTYRAG	2 cut(s)	71, 255
Sse9I	AATT	2 cut(s)	91, 361
SsiI	CCGC	2 cut(s)	11, 52
SspI	AATATT	2 cut(s)	136, 179
SstI	GAGCTC	1 cut(s)	72
StyD4I	CCNGG	2 cut(s)	418, 437
StyI	CCWWGG	2 cut(s)	399, 449
TaqI	TCGA	1 cut(s)	102
TasI	AATT	2 cut(s)	91, 361
Tru1I	TTAA	2 cut(s)	62, 89
Tru9I	TTAA	2 cut(s)	62, 89
TscAI	CASTG	1 cut(s)	373
TseI	GCWGC	2 cut(s)	54, 283
TspRI	CASTG	1 cut(s)	373
VpaK11BI	GGWCC	1 cut(s)	435
XapI	RAATTY	1 cut(s)	361

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.