FvH4_7g33890

Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb7
OG0013227
Physical Location & Seq
Forward (+)
24011395 .. 24014480
3086 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_7g33890.t1

Sequence Viewer

Length: 471 bp
ATGACGACGGTGGTGAAGAGCATAGTGCAGGCGATCAAAGAGAAAGGTCTGCGCAATTTCTTAAGGCAGGCCAGGGAAGAAGGTTACCTAAAATGTCTACCTGATGGAAACCTTCTGCAAACTAAAATCCACAATATCGGGGCAAAGCTTGTTGGTGTTGATAAAGTTGGGAACAAATATTATGAGAATCTTAACACTCAATATGGAAGACACAGGTGGGTTGAATATGCAGAGAAAGGTCGCTACAATGCTTCTCAAGTGCCACCAGAATGGCATGGTTGGCTGCACTACATAACAGATCATACTGGAGACGAGCTTCTGATGCTAAAGCCACAGAGGTATCACCTTGAACACAGGGAGAATTTCACTGGTGAAGGTGATGAGTTTATCTACCACTCCAAAGGGCACAGCCTCAATCCAGGGCAGAGAGACTGGACCAGGTACCAGCCTTGGGAGCCAACAAAACCCTGA

Protein Analysis

157

Amino Acids

18.39

Weight (kDa)

8.89

Isoelectric Point (pI)

26.48

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NDUFA12 PF05071 49 - 153 9e-27 NADH ubiquinone oxidoreductase subunit NDUFA12
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0013227)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G03100 AT3G03100
fragaria_vesca FvH4_7g33890
malus_domestica MD01G1235400.v1.1 MD07G1308200.v1.1
prunus_persica Prupe.2G325400_v2.0.a1 Prupe.2G325400_v2.0.a1
pyrus_communis pycom07g28040
rosa_chinensis RchiOBHm_Chr1g0384091
rosa_laevigata RLG00000026073
rosa_multiflora Rmu_sc0005139.1_g000018
rosa_roxburghii Rroxscaffold_4G00277000
rosa_rugosa Rorug01G0444700
rosa_samantha Rh1AG470100 Rh1BG427600 Rh1CG440300 Rh1DG460600
rosa_wichuraiana Rw1G040250

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 53
Acc65I GGTACC 1 cut(s) 441
AccB1I GGYRCC 1 cut(s) 441
AccI GTMKAC 1 cut(s) 97
AcsI RAATTY 1 cut(s) 361
AfaI GTAC 1 cut(s) 443
AfiI CCNNNNNNNGG 1 cut(s) 451
AflII CTTAAG 1 cut(s) 61
AgsI TTSAA 2 cut(s) 224, 350
AjnI CCWGG 3 cut(s) 71, 418, 437
AluBI AGCT 2 cut(s) 148, 316
AluI AGCT 2 cut(s) 148, 316
Alw26I GTCTC 2 cut(s) 303, 423
AoxI GGCC 1 cut(s) 69
ApeKI GCWGC 1 cut(s) 283
ApoI RAATTY 1 cut(s) 361
Asp718I GGTACC 1 cut(s) 441
AspLEI GCGC 1 cut(s) 54
AspS9I GGNCC 1 cut(s) 435
AsuHPI GGTGA 4 cut(s) 25, 335, 383, 389
AvaII GGWCC 1 cut(s) 435
BaeGI GKGCMC 1 cut(s) 408
BaeI ACNNNNGTAYC 2 cut(s) 323, 356
BanI GGYRCC 1 cut(s) 441
BbsI GAAGAC 1 cut(s) 214
BbvI GCAGC 1 cut(s) 270
BccI CCATC 1 cut(s) 98
BciT130I CCWGG 3 cut(s) 73, 420, 439
BcoDI GTCTC 2 cut(s) 303, 423
BfrI CTTAAG 1 cut(s) 61
BisI GCNGC 1 cut(s) 284
BlsI GCNGC 1 cut(s) 285
Bme1390I CCNGG 3 cut(s) 73, 420, 439
Bme18I GGWCC 1 cut(s) 435
BmgT120I GGNCC 1 cut(s) 435
BmiI GGNNCC 2 cut(s) 443, 456
BmrFI CCNGG 3 cut(s) 73, 420, 439
BmsI GCATC 1 cut(s) 312
BpiI GAAGAC 1 cut(s) 214
BpmI CTGGAG 1 cut(s) 327
BpuEI CTTGAG 1 cut(s) 240
BsaJI CCNNGG 3 cut(s) 72, 419, 449
Bsc4I CCNNNNNNNGG 1 cut(s) 451
Bse1I ACTGG 3 cut(s) 310, 373, 437
BseBI CCWGG 3 cut(s) 73, 420, 439
BseDI CCNNGG 3 cut(s) 72, 419, 449
BseLI CCNNNNNNNGG 1 cut(s) 451
BseNI ACTGG 3 cut(s) 310, 373, 437
BseSI GKGCMC 1 cut(s) 408
BseXI GCAGC 1 cut(s) 270
BsgI GTGCAG 2 cut(s) 47, 269
BshFI GGCC 1 cut(s) 71
BshNI GGYRCC 1 cut(s) 441
BslI CCNNNNNNNGG 1 cut(s) 451
BsmAI GTCTC 2 cut(s) 303, 423
BsmBI CGTCTC 1 cut(s) 303
BsnI GGCC 1 cut(s) 71
Bsp1286I GDGCHC 1 cut(s) 408
Bsp143I GATC 2 cut(s) 33, 298
BspANI GGCC 1 cut(s) 71
BspLI GGNNCC 2 cut(s) 443, 456
BspQI GCTCTTC 1 cut(s) 11
BspT107I GGYRCC 1 cut(s) 441
BspTI CTTAAG 1 cut(s) 61
BsrI ACTGG 3 cut(s) 310, 373, 437
BssECI CCNNGG 3 cut(s) 72, 419, 449
BssMI GATC 2 cut(s) 33, 298
BssT1I CCWWGG 1 cut(s) 449
Bst2UI CCWGG 3 cut(s) 73, 420, 439
Bst4CI ACNGT 1 cut(s) 10
Bst6I CTCTTC 1 cut(s) 11
BstAFI CTTAAG 1 cut(s) 61
BstC8I GCNNGC 2 cut(s) 30, 69
BstEII GGTNACC 1 cut(s) 83
BstHHI GCGC 1 cut(s) 54
BstKTI GATC 2 cut(s) 36, 301
BstMAI GTCTC 2 cut(s) 303, 423
BstMBI GATC 2 cut(s) 33, 298
BstMWI GCNNNNNNNGC 3 cut(s) 280, 322, 454
BstNI CCWGG 3 cut(s) 73, 420, 439
BstPI GGTNACC 1 cut(s) 83
BstSCI CCNGG 3 cut(s) 71, 418, 437
BstSLI GKGCMC 1 cut(s) 408
BstV1I GCAGC 1 cut(s) 270
BstV2I GAAGAC 1 cut(s) 214
BstXI CCANNNNNNTGG 1 cut(s) 270
BsuRI GGCC 1 cut(s) 71
BtsIMutI CAGTG 1 cut(s) 366
Cac8I GCNNGC 2 cut(s) 30, 69
CfoI GCGC 1 cut(s) 54
Cfr13I GGNCC 1 cut(s) 435
CsiI ACCWGGT 1 cut(s) 437
Csp6I GTAC 1 cut(s) 442
CviAII CATG 1 cut(s) 275
CviJI RGCY 8 cut(s) 71, 148, 283, 316, 331, 411, 448, 457
CviKI_1 RGCY 8 cut(s) 71, 148, 283, 316, 331, 411, 448, 457
CviQI GTAC 1 cut(s) 442
DpnI GATC 2 cut(s) 35, 300
DpnII GATC 2 cut(s) 33, 298
Eam1104I CTCTTC 1 cut(s) 11
EarI CTCTTC 1 cut(s) 11
Eco130I CCWWGG 1 cut(s) 449
Eco47I GGWCC 1 cut(s) 435
Eco91I GGTNACC 1 cut(s) 83
EcoO65I GGTNACC 1 cut(s) 83
EcoRII CCWGG 3 cut(s) 71, 418, 437
EcoT14I CCWWGG 1 cut(s) 449
ErhI CCWWGG 1 cut(s) 449
Esp3I CGTCTC 1 cut(s) 303
FaeI CATG 1 cut(s) 278
FaiI YATR 7 cut(s) 23, 183, 204, 228, 276, 293, 303
FatI CATG 1 cut(s) 274
FblI GTMKAC 1 cut(s) 97
Fnu4HI GCNGC 1 cut(s) 284
Fsp4HI GCNGC 1 cut(s) 284
FspI TGCGCA 1 cut(s) 53
GlaI GCGC 1 cut(s) 53
GluI GCNGC 1 cut(s) 284
GsuI CTGGAG 1 cut(s) 327
HaeIII GGCC 1 cut(s) 71
HhaI GCGC 1 cut(s) 54
Hin1II CATG 1 cut(s) 278
Hin6I GCGC 1 cut(s) 52
HinP1I GCGC 1 cut(s) 52
HindIII AAGCTT 1 cut(s) 146
HinfI GANTC 1 cut(s) 187
HphI GGTGA 4 cut(s) 25, 335, 383, 389
Hpy166II GTNNAC 1 cut(s) 98
Hpy188I TCNGA 1 cut(s) 321
Hpy8I GTNNAC 1 cut(s) 98
Hpy99I CGWCG 1 cut(s) 10
HpyAV CCTTC 3 cut(s) 74, 122, 368
HpyCH4III ACNGT 1 cut(s) 10
HpyCH4V TGCA 4 cut(s) 28, 118, 230, 286
HpyF10VI GCNNNNNNNGC 3 cut(s) 280, 322, 454
Hsp92II CATG 1 cut(s) 278
HspAI GCGC 1 cut(s) 52
KpnI GGTACC 1 cut(s) 445
Kzo9I GATC 2 cut(s) 33, 298
LguI GCTCTTC 1 cut(s) 11
LmnI GCTCC 1 cut(s) 454
Lsp1109I GCAGC 1 cut(s) 270
LweI GCATC 1 cut(s) 312
MabI ACCWGGT 1 cut(s) 437
MaeIII GTNAC 1 cut(s) 83
MalI GATC 2 cut(s) 35, 300
MboI GATC 2 cut(s) 33, 298
MboII GAAGA 3 cut(s) 28, 89, 219
MhlI GDGCHC 1 cut(s) 408
MluCI AATT 2 cut(s) 55, 361
MnlI CCTC 2 cut(s) 330, 422
MseI TTAA 2 cut(s) 62, 192
MslI CAYNNNNRTG 1 cut(s) 268
MspCI CTTAAG 1 cut(s) 61
MspR9I CCNGG 3 cut(s) 73, 420, 439
MvaI CCWGG 3 cut(s) 73, 420, 439
MwoI GCNNNNNNNGC 3 cut(s) 280, 322, 454
NdeII GATC 2 cut(s) 33, 298
NlaIII CATG 1 cut(s) 278
NlaIV GGNNCC 2 cut(s) 443, 456
NsbI TGCGCA 1 cut(s) 53
PciSI GCTCTTC 1 cut(s) 11
PfeI GAWTC 1 cut(s) 187
PkrI GCNGC 1 cut(s) 285
Psp6I CCWGG 3 cut(s) 71, 418, 437
PspEI GGTNACC 1 cut(s) 83
PspGI CCWGG 3 cut(s) 71, 418, 437
PspN4I GGNNCC 2 cut(s) 443, 456
PspPI GGNCC 1 cut(s) 435
RsaI GTAC 1 cut(s) 443
RsaNI GTAC 1 cut(s) 442
RseI CAYNNNNRTG 1 cut(s) 268
SapI GCTCTTC 1 cut(s) 11
SaqAI TTAA 2 cut(s) 62, 192
SatI GCNGC 1 cut(s) 284
Sau3AI GATC 2 cut(s) 33, 298
Sau96I GGNCC 1 cut(s) 435
ScrFI CCNGG 3 cut(s) 73, 420, 439
SduI GDGCHC 1 cut(s) 408
SexAI ACCWGGT 1 cut(s) 437
SfaNI GCATC 1 cut(s) 312
SinI GGWCC 1 cut(s) 435
SmiMI CAYNNNNRTG 1 cut(s) 268
SmlI CTYRAG 2 cut(s) 61, 255
SmoI CTYRAG 2 cut(s) 61, 255
Sse9I AATT 2 cut(s) 55, 361
SspI AATATT 1 cut(s) 179
StyD4I CCNGG 3 cut(s) 71, 418, 437
StyI CCWWGG 1 cut(s) 449
TaaI ACNGT 1 cut(s) 10
TasI AATT 2 cut(s) 55, 361
TfiI GAWTC 1 cut(s) 187
Tru1I TTAA 2 cut(s) 62, 192
Tru9I TTAA 2 cut(s) 62, 192
TscAI CASTG 1 cut(s) 373
TseI GCWGC 1 cut(s) 283
TspRI CASTG 1 cut(s) 373
Vha464I CTTAAG 1 cut(s) 61
VpaK11BI GGWCC 1 cut(s) 435
XapI RAATTY 1 cut(s) 361
XmiI GTMKAC 1 cut(s) 97
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.