AT3G04050

Belongs to the pyruvate kinase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
3
OG0016515
Physical Location & Seq
Forward (+)
1049653 .. 1051608
1956 bp
Loading structure...
UTR
Exon/CDS
Intron
AT3G04050.1

Sequence Viewer

Length: 1533 bp
ATGGAGAAGTTACTTGCTGGACAAACAAACAATGGATCCCTCAAGTCAAAGACGAAGATCGTCTGTACTCTCGGACCGGCTTCGAGATCGGTTGAGATGGTTGAGAAGCTTCTCAAAGCCGGTATGAACGTAGCCCGGTTCAACTTCTCCCATGGTTCTCACTCATACCATCAAGAAACTCTTGACAATCTCAGAACCGCCATGGAGAACACTTGTATTCCCTGTGCCGTCATGCTCGACACCAAGGGTCCTGAGATCCGAACCGGGTTTCTCAAAGAAGGCAAACCGGTTGAGCTAATTCAAGGTCAAGAGATTACAATCTCAACTGATTACACCATGGAAGGAGATTCAAACACAATCTCAATGAGTTACAAGAAACTTGCTGAAGATCTCAAGTCCGGTGACGTGATTCTCTGTTCCGACGGTACAATCTCTCTTACCGTCTTGTCTTGTGACAAGAATCTCGGTCTTGTTCGTGCCCGTTGCGAGAACTCTGCAGTTCTTGGAGAAAGAAAGAACGTGAACCTCCCTGGAATCGTAGTTGATCTTCCAACACTCACAGAGAAGGATCAAGAGGATATTCTCCAATGGGGAGTTCCGAATAAGATCGATATCATCGCTCTTTCCTTTGTTCGTAAAGGATCTGATCTGGTCGAGGTCAGGAAGTTGCTTGGAGAGAACGCAAAGAGTATCATGCTCATGTCAAAGGTGGAGAATCAAGAAGGAGTGATGAATTTTGACAAGATTCTGGAATATTCTGATGCATTCATGGTGGCTAGAGGTGATTTAGGAATGGAGATTCCGATTGAAAAGATGTTTCTTGCTCAGAAGATGATGATTCAGAAGGCTAATGCTCTTGGGAAGCCAATCGTCACAGCCACACAGATGCTTGAGTCAATGACCAAATCTCCTCGTCCAACTAGAGCAGAAGCCACCGATGTAGCTAACGCTGTCCTTGACGGCACTGATTGCGTCATGCTCAGTGGAGAAACCGCCGCCGGAGCTCACCCTGAAACCGCCGTGCTAACAATGTCAAGAATCTGTAAGGAAGCAGAGGATTTCATCGATTACGACACAATGCACAAGAAGATTCAAGATATCGTTTCGTTGCCTTTATCTCCTATTGAGAGCTTGGCCGCTTCAGCCGTTTCGACGGCGAGGAGTCTTTGTGCGGCTGCGATTGTCGTTCTAACCAAGGGAGGTTACACGGTGGAGCTTGTGGCCAAGTACAGGCCTAGTGTTCCGATTCTGTCCGTAATTGTGCCGGAGATTACTCGTACGGATGATTTCGAGTGGTCGTGCTCTGAATCTGCGGCTCATGTGGCAAGACGTGGTTTGATCTACCGTGGAATTGTTCCTGTGATGGCGACGGGAGCATCCGCTAGGTCTTCGAACAAGGACTCGACGGAGGAGACGATTCAATTCGCCATTGAATTCGCGAAGAAGAAGGGAATATGTAAGACAGGAGACTCGATTGTGGCGTTGCACAAGATCGATGGCTCCTCTGTTGTCAAGATTTTGAACGTGGAGTAG

Protein Analysis

510

Amino Acids

55.29

Weight (kDa)

5.72

Isoelectric Point (pI)

33.14

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PK PF00224 17 - 341 1.2e-143 Pyruvate kinase, barrel domain
PK_C PF02887 376 - 498 8.4e-23 Pyruvate kinase, alpha/beta domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016515)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G04050 AT3G25960 AT3G55650
fragaria_vesca FvH4_3g14970
malus_domestica MD10G1210700.v1.1
prunus_persica Prupe.4G134000_v2.0.a1
rosa_chinensis RchiOBHm_Chr5g0024561
rosa_laevigata RLG00000032807
rosa_multiflora Rmu_sc0005699.1_g000007
rosa_rugosa Rorug05G0084000
rosa_samantha Rh5AG175700 Rh5CG189100 Rh5DG174800
rosa_wichuraiana Rw5G015880

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 1439
AciI CCGC 8 cut(s) 198, 993, 996, 1017, 1137, 1172, 1313, 1380
AclWI GGATC 5 cut(s) 30, 43, 250, 576, 649
AcoI YGGCCR 2 cut(s) 1134, 1221
AcsI RAATTY 2 cut(s) 733, 1433
AcuI CTGAAG 2 cut(s) 405, 1125
AfaI GTAC 4 cut(s) 67, 427, 1229, 1279
AfiI CCNNNNNNNGG 1 cut(s) 1230
AgeI ACCGGT 1 cut(s) 286
AgsI TTSAA 8 cut(s) 142, 302, 351, 809, 1094, 1421, 1433, 1522
AjiI CACGTC 2 cut(s) 406, 1331
AjnI CCWGG 1 cut(s) 529
AjuI GAANNNNNNNTTGG 2 cut(s) 579, 611
AluBI AGCT 6 cut(s) 109, 295, 944, 1004, 1131, 1216
AluI AGCT 6 cut(s) 109, 295, 944, 1004, 1131, 1216
Alw21I GWGCWC 2 cut(s) 1006, 1304
Alw26I GTCTC 2 cut(s) 1406, 1461
AlwI GGATC 5 cut(s) 30, 43, 250, 576, 649
AoxI GGCC 3 cut(s) 1134, 1221, 1232
ApeKI GCWGC 1 cut(s) 1175
ApoI RAATTY 2 cut(s) 733, 1433
AsiGI ACCGGT 1 cut(s) 286
AspS9I GGNCC 2 cut(s) 74, 248
AsuC2I CCSGG 2 cut(s) 136, 265
AsuHPI GGTGA 3 cut(s) 413, 794, 998
AsuII TTCGAA 1 cut(s) 1391
AvaII GGWCC 2 cut(s) 74, 248
BaeGI GKGCMC 1 cut(s) 481
BalI TGGCCA 1 cut(s) 1223
BamHI GGATCC 1 cut(s) 35
BanII GRGCYC 1 cut(s) 1006
BarI GAAGNNNNNNTAC 2 cut(s) 531, 563
BbsI GAAGAC 1 cut(s) 1380
Bbv12I GWGCWC 2 cut(s) 1006, 1304
BbvI GCAGC 1 cut(s) 1162
BccI CCATC 4 cut(s) 91, 177, 1357, 1490
BceAI ACGGC 5 cut(s) 212, 976, 1004, 1130, 1170
BcgI CGANNNNNNTGC 2 cut(s) 476, 510
BciT130I CCWGG 1 cut(s) 531
BcnI CCSGG 2 cut(s) 136, 265
BcoDI GTCTC 2 cut(s) 1406, 1461
BfaI CTAG 4 cut(s) 777, 921, 1236, 1383
BfmI CTRYAG 1 cut(s) 495
BglII AGATCT 1 cut(s) 388
BisI GCNGC 5 cut(s) 996, 1137, 1173, 1176, 1314
BlsI GCNGC 5 cut(s) 997, 1138, 1174, 1177, 1315
Bme1390I CCNGG 3 cut(s) 136, 265, 531
Bme18I GGWCC 2 cut(s) 74, 248
BmgBI CACGTC 2 cut(s) 406, 1331
BmgT120I GGNCC 2 cut(s) 74, 248
BmiI GGNNCC 3 cut(s) 37, 249, 1501
BmrFI CCNGG 3 cut(s) 136, 265, 531
BmsI GCATC 3 cut(s) 751, 876, 1385
BpiI GAAGAC 1 cut(s) 1380
Bpu14I TTCGAA 1 cut(s) 1391
BpuEI CTTGAG 3 cut(s) 26, 377, 911
BpuMI CCSGG 2 cut(s) 136, 265
Bsa29I ATCGAT 3 cut(s) 609, 1065, 1494
BsaBI GATNNNNATC 2 cut(s) 317, 611
BsaJI CCNNGG 7 cut(s) 151, 201, 243, 336, 529, 1194, 1345
BsaWI WCCGGW 2 cut(s) 286, 398
BsaXI ACNNNNNCTCC 4 cut(s) 892, 922, 1153, 1183
Bsc4I CCNNNNNNNGG 1 cut(s) 1230
Bse118I RCCGGY 3 cut(s) 76, 119, 286
Bse8I GATNNNNATC 2 cut(s) 317, 611
BseBI CCWGG 1 cut(s) 531
BseCI ATCGAT 3 cut(s) 609, 1065, 1494
BseDI CCNNGG 7 cut(s) 151, 201, 243, 336, 529, 1194, 1345
BseGI GGATG 2 cut(s) 1288, 1376
BseJI GATNNNNATC 2 cut(s) 317, 611
BseLI CCNNNNNNNGG 1 cut(s) 1230
BseMII CTCAG 4 cut(s) 205, 243, 839, 994
BseRI GAGGAG 4 cut(s) 900, 1174, 1424, 1492
BseSI GKGCMC 1 cut(s) 481
BseXI GCAGC 1 cut(s) 1162
Bsh1236I CGCG 1 cut(s) 1439
BshFI GGCC 3 cut(s) 1136, 1223, 1234
BshTI ACCGGT 1 cut(s) 286
BshVI ATCGAT 3 cut(s) 609, 1065, 1494
BsiHKAI GWGCWC 2 cut(s) 1006, 1304
BsiSI CCGG 8 cut(s) 77, 120, 136, 264, 287, 399, 999, 1265
BsiWI CGTACG 1 cut(s) 1277
BslI CCNNNNNNNGG 1 cut(s) 1230
BsmAI GTCTC 2 cut(s) 1406, 1461
BsmBI CGTCTC 1 cut(s) 1406
BsmI GAATGC 1 cut(s) 764
BsnI GGCC 3 cut(s) 1136, 1223, 1234
Bsp119I TTCGAA 1 cut(s) 1391
Bsp1286I GDGCHC 3 cut(s) 481, 1006, 1304
Bsp19I CCATGG 3 cut(s) 151, 201, 336
Bsp68I TCGCGA 1 cut(s) 1439
BspACI CCGC 8 cut(s) 198, 993, 996, 1017, 1137, 1172, 1313, 1380
BspANI GGCC 3 cut(s) 1136, 1223, 1234
BspCNI CTCAG 4 cut(s) 204, 244, 838, 993
BspDI ATCGAT 3 cut(s) 609, 1065, 1494
BspFNI CGCG 1 cut(s) 1439
BspLI GGNNCC 3 cut(s) 37, 249, 1501
BspMAI CTGCAG 1 cut(s) 499
BspPI GGATC 5 cut(s) 30, 43, 250, 576, 649
BspT104I TTCGAA 1 cut(s) 1391
BsrFI RCCGGY 3 cut(s) 76, 119, 286
BssAI RCCGGY 3 cut(s) 76, 119, 286
BssECI CCNNGG 7 cut(s) 151, 201, 243, 336, 529, 1194, 1345
BssT1I CCWWGG 5 cut(s) 151, 201, 243, 336, 1194
Bst2UI CCWGG 1 cut(s) 531
Bst4CI ACNGT 4 cut(s) 425, 442, 1210, 1346
BstAPI GCANNNNNTGC 1 cut(s) 969
BstBI TTCGAA 1 cut(s) 1391
BstDEI CTNAG 4 cut(s) 191, 252, 825, 980
BstDSI CCRYGG 4 cut(s) 151, 201, 336, 1345
BstF5I GGATG 2 cut(s) 1288, 1376
BstFNI CGCG 1 cut(s) 1439
BstMAI GTCTC 2 cut(s) 1406, 1461
BstMWI GCNNNNNNNGC 5 cut(s) 969, 1001, 1142, 1322, 1373
BstNI CCWGG 1 cut(s) 531
BstSCI CCNGG 3 cut(s) 134, 263, 529
BstSFI CTRYAG 1 cut(s) 495
BstSLI GKGCMC 1 cut(s) 481
BstUI CGCG 1 cut(s) 1439
BstV1I GCAGC 1 cut(s) 1162
BstV2I GAAGAC 1 cut(s) 1380
BstX2I RGATCY 4 cut(s) 35, 255, 388, 641
BstYI RGATCY 4 cut(s) 35, 255, 388, 641
Bsu15I ATCGAT 3 cut(s) 609, 1065, 1494
BsuRI GGCC 3 cut(s) 1136, 1223, 1234
BsuTUI ATCGAT 3 cut(s) 609, 1065, 1494
BtgI CCRYGG 4 cut(s) 151, 201, 336, 1345
BtgZI GCGATG 1 cut(s) 601
BtrI CACGTC 2 cut(s) 406, 1331
BtsCI GGATG 2 cut(s) 1288, 1376
BtsIMutI CAGTG 2 cut(s) 963, 988
BtuMI TCGCGA 1 cut(s) 1439
Cfr10I RCCGGY 3 cut(s) 76, 119, 286
Cfr13I GGNCC 2 cut(s) 74, 248
ClaI ATCGAT 3 cut(s) 609, 1065, 1494
CpoI CGGWCCG 1 cut(s) 74
CseI GACGC 1 cut(s) 961
Csp6I GTAC 4 cut(s) 66, 426, 1228, 1278
CspAI ACCGGT 1 cut(s) 286
CspI CGGWCCG 1 cut(s) 74
CviAII CATG 9 cut(s) 152, 202, 232, 337, 694, 700, 769, 976, 1319
CviQI GTAC 4 cut(s) 66, 426, 1228, 1278
DdeI CTNAG 4 cut(s) 191, 252, 825, 980
EaeI YGGCCR 2 cut(s) 1134, 1221
Ecl136II GAGCTC 1 cut(s) 1004
Eco130I CCWWGG 5 cut(s) 151, 201, 243, 336, 1194
Eco147I AGGCCT 1 cut(s) 1234
Eco24I GRGCYC 1 cut(s) 1006
Eco32I GATATC 2 cut(s) 613, 1099
Eco47I GGWCC 2 cut(s) 74, 248
Eco53kI GAGCTC 1 cut(s) 1004
Eco57I CTGAAG 2 cut(s) 405, 1125
EcoICRI GAGCTC 1 cut(s) 1004
EcoO109I RGGNCCY 1 cut(s) 248
EcoRI GAATTC 1 cut(s) 1433
EcoRII CCWGG 1 cut(s) 529
EcoRV GATATC 2 cut(s) 613, 1099
EcoT14I CCWWGG 5 cut(s) 151, 201, 243, 336, 1194
EcoT22I ATGCAT 1 cut(s) 766
EcoT38I GRGCYC 1 cut(s) 1006
ErhI CCWWGG 5 cut(s) 151, 201, 243, 336, 1194
Esp3I CGTCTC 1 cut(s) 1406
FaeI CATG 9 cut(s) 155, 205, 235, 340, 697, 703, 772, 979, 1322
FalI AAGNNNNNCTT 2 cut(s) 165, 197
FatI CATG 9 cut(s) 151, 201, 231, 336, 693, 699, 768, 975, 1318
Fnu4HI GCNGC 5 cut(s) 996, 1137, 1173, 1176, 1314
FokI GGATG 2 cut(s) 1295, 1363
FriOI GRGCYC 1 cut(s) 1006
Fsp4HI GCNGC 5 cut(s) 996, 1137, 1173, 1176, 1314
FspBI CTAG 4 cut(s) 777, 921, 1236, 1383
GluI GCNGC 5 cut(s) 996, 1137, 1173, 1176, 1314
HaeIII GGCC 3 cut(s) 1136, 1223, 1234
HapII CCGG 8 cut(s) 77, 120, 136, 264, 287, 399, 999, 1265
HgaI GACGC 1 cut(s) 961
Hin1II CATG 9 cut(s) 155, 205, 235, 340, 697, 703, 772, 979, 1322
HindIII AAGCTT 1 cut(s) 107
HpaII CCGG 8 cut(s) 77, 120, 136, 264, 287, 399, 999, 1265
HphI GGTGA 3 cut(s) 413, 794, 998
Hpy166II GTNNAC 1 cut(s) 523
Hpy8I GTNNAC 1 cut(s) 523
Hpy99I CGWCG 4 cut(s) 425, 1156, 1372, 1408
HpyAV CCTTC 6 cut(s) 272, 335, 559, 716, 838, 1441
HpyCH4III ACNGT 4 cut(s) 425, 442, 1210, 1346
HpyCH4IV ACGT 5 cut(s) 129, 405, 519, 1330, 1524
HpyCH4V TGCA 4 cut(s) 497, 764, 1081, 1486
HpyF10VI GCNNNNNNNGC 5 cut(s) 969, 1001, 1142, 1322, 1373
HpyF3I CTNAG 4 cut(s) 191, 252, 825, 980
HpySE526I ACGT 5 cut(s) 129, 405, 519, 1330, 1524
Hsp92II CATG 9 cut(s) 155, 205, 235, 340, 697, 703, 772, 979, 1322
LmnI GCTCC 4 cut(s) 1001, 1213, 1373, 1505
Lsp1109I GCAGC 1 cut(s) 1162
LweI GCATC 3 cut(s) 751, 876, 1385
MaeI CTAG 4 cut(s) 777, 921, 1236, 1383
MaeII ACGT 5 cut(s) 129, 405, 519, 1330, 1524
MaeIII GTNAC 6 cut(s) 9, 368, 401, 452, 871, 1202
MboII GAAGA 8 cut(s) 67, 398, 539, 841, 1099, 1380, 1453, 1456
MflI RGATCY 4 cut(s) 35, 255, 388, 641
MhlI GDGCHC 3 cut(s) 481, 1006, 1304
MlsI TGGCCA 1 cut(s) 1223
MluCI AATT 6 cut(s) 297, 733, 1257, 1350, 1421, 1433
MluNI TGGCCA 1 cut(s) 1223
MlyI GAGTC 4 cut(s) 902, 1171, 1394, 1463
MmeI TCCRAC 3 cut(s) 444, 575, 941
Mox20I TGGCCA 1 cut(s) 1223
Mph1103I ATGCAT 1 cut(s) 766
MscI TGGCCA 1 cut(s) 1223
MslI CAYNNNNRTG 2 cut(s) 698, 884
Msp20I TGGCCA 1 cut(s) 1223
MspI CCGG 8 cut(s) 77, 120, 136, 264, 287, 399, 999, 1265
MspR9I CCNGG 3 cut(s) 136, 265, 531
Mva1269I GAATGC 1 cut(s) 764
MvaI CCWGG 1 cut(s) 531
MvnI CGCG 1 cut(s) 1439
MwoI GCNNNNNNNGC 5 cut(s) 969, 1001, 1142, 1322, 1373
NciI CCSGG 2 cut(s) 136, 265
NcoI CCATGG 3 cut(s) 151, 201, 336
NlaIII CATG 9 cut(s) 155, 205, 235, 340, 697, 703, 772, 979, 1322
NlaIV GGNNCC 3 cut(s) 37, 249, 1501
NmuCI GTSAC 3 cut(s) 401, 452, 871
NruI TCGCGA 1 cut(s) 1439
NsiI ATGCAT 1 cut(s) 766
NspV TTCGAA 1 cut(s) 1391
PceI AGGCCT 1 cut(s) 1234
PcsI WCGNNNNNNNCGW 2 cut(s) 1412, 1478
PctI GAATGC 1 cut(s) 764
Pfl23II CGTACG 1 cut(s) 1277
PinAI ACCGGT 1 cut(s) 286
PkrI GCNGC 5 cut(s) 997, 1138, 1174, 1177, 1315
PleI GAGTC 4 cut(s) 901, 1170, 1394, 1463
PpsI GAGTC 4 cut(s) 901, 1170, 1394, 1463
PpuMI RGGWCCY 1 cut(s) 248
Psp124BI GAGCTC 1 cut(s) 1006
Psp5II RGGWCCY 1 cut(s) 248
Psp6I CCWGG 1 cut(s) 529
PspGI CCWGG 1 cut(s) 529
PspLI CGTACG 1 cut(s) 1277
PspN4I GGNNCC 3 cut(s) 37, 249, 1501
PspPI GGNCC 2 cut(s) 74, 248
PspPPI RGGWCCY 1 cut(s) 248
PstI CTGCAG 1 cut(s) 499
PsuI RGATCY 4 cut(s) 35, 255, 388, 641
RruI TCGCGA 1 cut(s) 1439
RsaI GTAC 4 cut(s) 67, 427, 1229, 1279
RsaNI GTAC 4 cut(s) 66, 426, 1228, 1278
RseI CAYNNNNRTG 2 cut(s) 698, 884
Rsr2I CGGWCCG 1 cut(s) 74
RsrII CGGWCCG 1 cut(s) 74
SacI GAGCTC 1 cut(s) 1006
SatI GCNGC 5 cut(s) 996, 1137, 1173, 1176, 1314
Sau96I GGNCC 2 cut(s) 74, 248
SchI GAGTC 4 cut(s) 902, 1171, 1394, 1463
ScrFI CCNGG 3 cut(s) 136, 265, 531
SduI GDGCHC 3 cut(s) 481, 1006, 1304
SfaNI GCATC 3 cut(s) 751, 876, 1385
SfcI CTRYAG 1 cut(s) 495
SfuI TTCGAA 1 cut(s) 1391
SinI GGWCC 2 cut(s) 74, 248
SmiMI CAYNNNNRTG 2 cut(s) 698, 884
SmlI CTYRAG 3 cut(s) 41, 392, 890
SmoI CTYRAG 3 cut(s) 41, 392, 890
Sse9I AATT 6 cut(s) 297, 733, 1257, 1350, 1421, 1433
SseBI AGGCCT 1 cut(s) 1234
SsiI CCGC 8 cut(s) 198, 993, 996, 1017, 1137, 1172, 1313, 1380
SspI AATATT 1 cut(s) 755
SspMI CTAG 4 cut(s) 777, 921, 1236, 1383
SstI GAGCTC 1 cut(s) 1006
StuI AGGCCT 1 cut(s) 1234
StyD4I CCNGG 3 cut(s) 134, 263, 529
StyI CCWWGG 5 cut(s) 151, 201, 243, 336, 1194
TaaI ACNGT 4 cut(s) 425, 442, 1210, 1346
TaiI ACGT 5 cut(s) 132, 408, 522, 1333, 1527
TaqII GACCGA 1 cut(s) 455
TasI AATT 6 cut(s) 297, 733, 1257, 1350, 1421, 1433
TatI WGTACW 2 cut(s) 65, 1227
TauI GCSGC 4 cut(s) 998, 1139, 1175, 1316
TscAI CASTG 2 cut(s) 970, 988
TseFI GTSAC 3 cut(s) 401, 452, 871
TseI GCWGC 1 cut(s) 1175
Tsp45I GTSAC 3 cut(s) 401, 452, 871
TspDTI ATGAA 4 cut(s) 140, 746, 757, 1051
TspGWI ACGGA 3 cut(s) 1243, 1295, 1421
TspRI CASTG 2 cut(s) 970, 988
VpaK11BI GGWCC 2 cut(s) 74, 248
XapI RAATTY 2 cut(s) 733, 1433
XspI CTAG 4 cut(s) 777, 921, 1236, 1383
Zsp2I ATGCAT 1 cut(s) 766
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.