AT3G55650

Belongs to the pyruvate kinase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
3
OG0016515
Physical Location & Seq
Forward (+)
20647085 .. 20648617
1533 bp
Loading structure...
UTR
Exon/CDS
Intron
AT3G55650.1

Sequence Viewer

Length: 1533 bp
ATGGAGATGTTACTTGGTGGACAAGCAACCAATGGAGCTCTTCGTTCAAAGACGAAGATAATTTGTACTCTTGGACCGGTTTCAAGATCGGTGGAGATGATTGAGAAGCTTCTCAAGGCTGGTATGAACGTAGCCCGGTTCAATTTCTCTCATGGTTCTCACTCTTACCATCAAGAAACTCTCGATAATCTCCGAACCGCCATGGACAACACTGGTATTCTCTCCGCCGTCATGCTCGACACAAAGGGTCCTGAGATACGAACCGGGTTTCTCAAAGAAGGCAAACCGATACAGCTAAACCAAGGTCAAGAGATCACCATCTCAATTGATTACATGATAGAAGGAGATTCAAACGTTATCTCCATGAGCTACAAGAAACTTGCAGAAGACGTCAAACCTGGTGATGTGATTCTCTGTTCAGACGGCACAATCTCTTTAACTGTCTTGTCCTGTGACAAGTCTTTCGGTCTTGTTCGTTGCCGTTGCGAGAACTCTGCAATTCTAGGAGAAAGAAAAAACGTTAATCTTCCTGGAATTGTAGTTGATCTCCCAACACTTACTGAGAAAGATAAAGAAGATATCATCCAATGGGGAGTTCCGAACAAAATCGACATCATCGCTCTTTCTTTTGTTCGTAAAGGATCTGACCTAACTGAAGTTAGGAGGTTACTTGGAGAACACTCAAAGAACATCATGCTCATGTCAAAGGTGGAGAATCAAGAAGGGGTCATGAATTGTGAAAAGATTCTGGAGAATTCTGATGCTTTCATGGTGGCTAGAGGAGATCTTGGAATGGAGATTCCGATCGAAAAGATGTTCCTTGCTCAGAAAACAATGATCAAGATGGCTAATGCTCTTGGGAAACCAGTTGTCACAGCCACACAGATGCTTGAATCCATGACAGTATCTCCTCGTCCGACTAGAGCTGAAGCCACTGACGTTGCAAACGCTGTTCTTGACGGGACAGACTGCGTTATGCTTAGTGGAGAAACCGCTGCTGGAGCTCACCCTGAGGCTGCCGTGCTAACAATGTCAAGGATCTGTAAAGAAGCAGAGGATTTCATCGATTACGACATTCTCCACAAGAAAACCCTAGGAATGGTTTCGTTACCGTTATCTCCGATCGAGAGCTTAGCTGCTTCAGTTGTTTCGACGGCACAGAGTGTTTTTGCGTCGGCGATCGTTGTTCTCACCAAAGGCGGTTACACGGCGGAGCTTGTGGCTAAATACAGGCCGAGCGTTCCGATTTTGTCAGTGATTGTGCCAGAGATTGCTCAAGGGAATGATATGGAGATGTCGTGCTCAGACTCGGTGGCTCATGCGGCGAGACGTGGTTTGATTTACCGTAGGATTATTCCGGTGGTGGCGACGGGATCTTCGGCTAGGGATTCAAACAAAGATGCAACGGAGGAGATGATTAATTTGGCTATCGGATTCGCGAAGACGAAGGGAATTTGCAAGAATGGAGATTCGATTGTGGCGTTGCACAAGATTGATGGTTCCTCTGTTGTTAAGATAGTGACCGTGGAGTAA

Protein Analysis

510

Amino Acids

54.98

Weight (kDa)

5.78

Isoelectric Point (pI)

38.56

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PK PF00224 17 - 341 2.5e-142 Pyruvate kinase, barrel domain
PK_C PF02887 376 - 499 1.2e-19 Pyruvate kinase, alpha/beta domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0016515)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G04050 AT3G25960 AT3G55650
fragaria_vesca FvH4_3g14970
malus_domestica MD10G1210700.v1.1
prunus_persica Prupe.4G134000_v2.0.a1
rosa_chinensis RchiOBHm_Chr5g0024561
rosa_laevigata RLG00000032807
rosa_multiflora Rmu_sc0005699.1_g000007
rosa_rugosa Rorug05G0084000
rosa_samantha Rh5AG175700 Rh5CG189100 Rh5DG174800
rosa_wichuraiana Rw5G015880

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 393
AccII CGCG 1 cut(s) 1439
AciI CCGC 6 cut(s) 198, 225, 993, 1200, 1211, 1322
AclI AACGTT 2 cut(s) 354, 519
AclWI GGATC 3 cut(s) 649, 1046, 1381
AcsI RAATTY 2 cut(s) 754, 1452
AcuI CTGAAG 3 cut(s) 675, 948, 1125
AcyI GRCGYC 1 cut(s) 390
AdeI CACNNNGTG 1 cut(s) 1163
AfaI GTAC 1 cut(s) 67
AfiI CCNNNNNNNGG 1 cut(s) 1099
AgeI ACCGGT 1 cut(s) 76
AgsI TTSAA 6 cut(s) 48, 84, 142, 351, 893, 1392
AjiI CACGTC 1 cut(s) 1331
AjnI CCWGG 2 cut(s) 397, 529
AjuI GAANNNNNNNTTGG 2 cut(s) 579, 611
AluBI AGCT 9 cut(s) 38, 109, 295, 369, 926, 1004, 1131, 1136, 1216
AluI AGCT 9 cut(s) 38, 109, 295, 369, 926, 1004, 1131, 1136, 1216
Alw21I GWGCWC 3 cut(s) 40, 1006, 1304
Alw26I GTCTC 1 cut(s) 1321
AlwI GGATC 3 cut(s) 649, 1046, 1381
AoxI GGCC 1 cut(s) 1232
ApeKI GCWGC 3 cut(s) 995, 1016, 1136
ApoI RAATTY 2 cut(s) 754, 1452
ArsI GACNNNNNNTTYG 4 cut(s) 446, 478, 1360, 1392
AseI ATTAAT 1 cut(s) 1419
AsiGI ACCGGT 1 cut(s) 76
Asp700I GAANNNNTTC 2 cut(s) 744, 1102
AspA2I CCTAGG 1 cut(s) 1093
AspS9I GGNCC 2 cut(s) 74, 248
AsuC2I CCSGG 2 cut(s) 136, 265
AsuHPI GGTGA 4 cut(s) 307, 413, 998, 1183
AvaII GGWCC 2 cut(s) 74, 248
AvrII CCTAGG 1 cut(s) 1093
AxyI CCTNAGG 1 cut(s) 1011
BanII GRGCYC 2 cut(s) 40, 1006
BbsI GAAGAC 2 cut(s) 393, 1448
Bbv12I GWGCWC 3 cut(s) 40, 1006, 1304
BbvI GCAGC 3 cut(s) 982, 1003, 1123
BccI CCATC 4 cut(s) 177, 326, 838, 1490
BceAI ACGGC 6 cut(s) 212, 439, 465, 1004, 1170, 1224
BcgI CGANNNNNNTGC 2 cut(s) 476, 510
BciT130I CCWGG 2 cut(s) 399, 531
BclI TGATCA 1 cut(s) 837
BcnI CCSGG 2 cut(s) 136, 265
BcoDI GTCTC 1 cut(s) 1321
BfaI CTAG 5 cut(s) 503, 777, 921, 1094, 1383
BglII AGATCT 1 cut(s) 784
BisI GCNGC 4 cut(s) 996, 1017, 1137, 1323
BlnI CCTAGG 1 cut(s) 1093
BlpI GCTNAGC 1 cut(s) 1132
BlsI GCNGC 4 cut(s) 997, 1018, 1138, 1324
Bme1390I CCNGG 4 cut(s) 136, 265, 399, 531
Bme18I GGWCC 2 cut(s) 74, 248
BmgBI CACGTC 1 cut(s) 1331
BmgT120I GGNCC 2 cut(s) 74, 248
BmiI GGNNCC 2 cut(s) 249, 1501
BmrFI CCNGG 4 cut(s) 136, 265, 399, 531
BmsI GCATC 3 cut(s) 751, 876, 1390
BpiI GAAGAC 2 cut(s) 393, 1448
BpmI CTGGAG 2 cut(s) 770, 1020
Bpu1102I GCTNAGC 1 cut(s) 1132
BpuEI CTTGAG 2 cut(s) 98, 1260
BpuMI CCSGG 2 cut(s) 136, 265
Bsa29I ATCGAT 1 cut(s) 1065
BsaBI GATNNNNATC 2 cut(s) 317, 803
BsaHI GRCGYC 1 cut(s) 390
BsaJI CCNNGG 4 cut(s) 201, 301, 1093, 1524
BsaWI WCCGGW 2 cut(s) 76, 1357
BsaXI ACNNNNNCTCC 6 cut(s) 344, 374, 892, 922, 1283, 1313
Bsc4I CCNNNNNNNGG 1 cut(s) 1099
Bse118I RCCGGY 1 cut(s) 76
Bse1I ACTGG 2 cut(s) 217, 866
Bse21I CCTNAGG 1 cut(s) 1011
Bse8I GATNNNNATC 2 cut(s) 317, 803
BseBI CCWGG 2 cut(s) 399, 531
BseCI ATCGAT 1 cut(s) 1065
BseDI CCNNGG 4 cut(s) 201, 301, 1093, 1524
BseGI GGATG 1 cut(s) 582
BseJI GATNNNNATC 2 cut(s) 317, 803
BseLI CCNNNNNNNGG 1 cut(s) 1099
BseMII CTCAG 5 cut(s) 243, 552, 839, 1002, 1317
BseNI ACTGG 2 cut(s) 217, 866
BseRI GAGGAG 3 cut(s) 795, 900, 1424
BseXI GCAGC 3 cut(s) 982, 1003, 1123
Bsh1236I CGCG 1 cut(s) 1439
Bsh1285I CGRYCG 3 cut(s) 807, 1125, 1182
BshFI GGCC 1 cut(s) 1234
BshTI ACCGGT 1 cut(s) 76
BshVI ATCGAT 1 cut(s) 1065
BsiEI CGRYCG 3 cut(s) 807, 1125, 1182
BsiHKAI GWGCWC 3 cut(s) 40, 1006, 1304
BsiSI CCGG 4 cut(s) 77, 136, 264, 1358
BslFI GGGAC 1 cut(s) 976
BslI CCNNNNNNNGG 1 cut(s) 1099
BsmAI GTCTC 1 cut(s) 1321
BsmBI CGTCTC 1 cut(s) 1321
BsmFI GGGAC 1 cut(s) 976
BsnI GGCC 1 cut(s) 1234
Bsp1286I GDGCHC 3 cut(s) 40, 1006, 1304
Bsp1720I GCTNAGC 1 cut(s) 1132
Bsp19I CCATGG 1 cut(s) 201
Bsp68I TCGCGA 1 cut(s) 1439
BspACI CCGC 6 cut(s) 198, 225, 993, 1200, 1211, 1322
BspANI GGCC 1 cut(s) 1234
BspCNI CTCAG 5 cut(s) 244, 553, 838, 1003, 1316
BspDI ATCGAT 1 cut(s) 1065
BspFNI CGCG 1 cut(s) 1439
BspHI TCATGA 1 cut(s) 729
BspLI GGNNCC 2 cut(s) 249, 1501
BspPI GGATC 3 cut(s) 649, 1046, 1381
BspQI GCTCTTC 1 cut(s) 45
BsrFI RCCGGY 1 cut(s) 76
BsrI ACTGG 2 cut(s) 217, 866
BssAI RCCGGY 1 cut(s) 76
BssECI CCNNGG 4 cut(s) 201, 301, 1093, 1524
BssNI GRCGYC 1 cut(s) 390
BssT1I CCWWGG 3 cut(s) 201, 301, 1093
Bst2UI CCWGG 2 cut(s) 399, 531
Bst4CI ACNGT 5 cut(s) 442, 904, 1113, 1346, 1525
Bst6I CTCTTC 1 cut(s) 45
BstACI GRCGYC 1 cut(s) 390
BstDEI CTNAG 7 cut(s) 252, 561, 825, 980, 1011, 1132, 1303
BstDSI CCRYGG 2 cut(s) 201, 1524
BstF5I GGATG 1 cut(s) 582
BstFNI CGCG 1 cut(s) 1439
BstMAI GTCTC 1 cut(s) 1321
BstMCI CGRYCG 3 cut(s) 807, 1125, 1182
BstMWI GCNNNNNNNGC 2 cut(s) 1001, 1322
BstNI CCWGG 2 cut(s) 399, 531
BstSCI CCNGG 4 cut(s) 134, 263, 397, 529
BstUI CGCG 1 cut(s) 1439
BstV1I GCAGC 3 cut(s) 982, 1003, 1123
BstV2I GAAGAC 2 cut(s) 393, 1448
BstX2I RGATCY 4 cut(s) 641, 784, 1038, 1373
BstYI RGATCY 4 cut(s) 641, 784, 1038, 1373
Bsu15I ATCGAT 1 cut(s) 1065
Bsu36I CCTNAGG 1 cut(s) 1011
BsuRI GGCC 1 cut(s) 1234
BsuTUI ATCGAT 1 cut(s) 1065
BtgI CCRYGG 2 cut(s) 201, 1524
BtgZI GCGATG 1 cut(s) 601
BtrI CACGTC 1 cut(s) 1331
BtsCI GGATG 1 cut(s) 582
BtsIMutI CAGTG 3 cut(s) 210, 933, 1260
BtuMI TCGCGA 1 cut(s) 1439
CciI TCATGA 1 cut(s) 729
Cfr10I RCCGGY 1 cut(s) 76
Cfr13I GGNCC 2 cut(s) 74, 248
ClaI ATCGAT 1 cut(s) 1065
CseI GACGC 1 cut(s) 1161
CsiI ACCWGGT 1 cut(s) 397
Csp6I GTAC 1 cut(s) 66
CspAI ACCGGT 1 cut(s) 76
CspCI CAANNNNNGTGG 4 cut(s) 72, 107, 922, 957
CviQI GTAC 1 cut(s) 66
DdeI CTNAG 7 cut(s) 252, 561, 825, 980, 1011, 1132, 1303
DraIII CACNNNGTG 1 cut(s) 1163
Eam1104I CTCTTC 1 cut(s) 45
EarI CTCTTC 1 cut(s) 45
EciI GGCGGA 2 cut(s) 214, 1226
Ecl136II GAGCTC 2 cut(s) 38, 1004
Eco130I CCWWGG 3 cut(s) 201, 301, 1093
Eco24I GRGCYC 2 cut(s) 40, 1006
Eco32I GATATC 1 cut(s) 580
Eco47I GGWCC 2 cut(s) 74, 248
Eco53kI GAGCTC 2 cut(s) 38, 1004
Eco57I CTGAAG 3 cut(s) 675, 948, 1125
Eco81I CCTNAGG 1 cut(s) 1011
EcoICRI GAGCTC 2 cut(s) 38, 1004
EcoO109I RGGNCCY 1 cut(s) 248
EcoRI GAATTC 1 cut(s) 754
EcoRII CCWGG 2 cut(s) 397, 529
EcoRV GATATC 1 cut(s) 580
EcoT14I CCWWGG 3 cut(s) 201, 301, 1093
EcoT38I GRGCYC 2 cut(s) 40, 1006
ErhI CCWWGG 3 cut(s) 201, 301, 1093
Esp3I CGTCTC 1 cut(s) 1321
FaqI GGGAC 1 cut(s) 976
FbaI TGATCA 1 cut(s) 837
Fnu4HI GCNGC 4 cut(s) 996, 1017, 1137, 1323
FokI GGATG 1 cut(s) 569
FriOI GRGCYC 2 cut(s) 40, 1006
Fsp4HI GCNGC 4 cut(s) 996, 1017, 1137, 1323
FspBI CTAG 5 cut(s) 503, 777, 921, 1094, 1383
GluI GCNGC 4 cut(s) 996, 1017, 1137, 1323
GsuI CTGGAG 2 cut(s) 770, 1020
HaeIII GGCC 1 cut(s) 1234
HapII CCGG 4 cut(s) 77, 136, 264, 1358
HgaI GACGC 1 cut(s) 1161
Hin1I GRCGYC 1 cut(s) 390
HindIII AAGCTT 1 cut(s) 107
HpaII CCGG 4 cut(s) 77, 136, 264, 1358
HphI GGTGA 4 cut(s) 307, 413, 998, 1183
Hpy166II GTNNAC 1 cut(s) 20
Hpy8I GTNNAC 1 cut(s) 20
Hpy99I CGWCG 3 cut(s) 1156, 1177, 1372
HpyAV CCTTC 4 cut(s) 272, 335, 716, 1441
HpyCH4III ACNGT 5 cut(s) 442, 904, 1113, 1346, 1525
HpyCH4IV ACGT 6 cut(s) 129, 354, 390, 519, 939, 1330
HpyCH4V TGCA 6 cut(s) 383, 497, 944, 1403, 1458, 1486
HpyF10VI GCNNNNNNNGC 2 cut(s) 1001, 1322
HpyF3I CTNAG 7 cut(s) 252, 561, 825, 980, 1011, 1132, 1303
HpySE526I ACGT 6 cut(s) 129, 354, 390, 519, 939, 1330
Hsp92I GRCGYC 1 cut(s) 390
Ksp22I TGATCA 1 cut(s) 837
LguI GCTCTTC 1 cut(s) 45
LmnI GCTCC 3 cut(s) 35, 1001, 1213
Lsp1109I GCAGC 3 cut(s) 982, 1003, 1123
LweI GCATC 3 cut(s) 751, 876, 1390
MabI ACCWGGT 1 cut(s) 397
MaeI CTAG 5 cut(s) 503, 777, 921, 1094, 1383
MaeII ACGT 6 cut(s) 129, 354, 390, 519, 939, 1330
MaeIII GTNAC 7 cut(s) 9, 452, 666, 871, 1107, 1202, 1519
MboII GAAGA 7 cut(s) 32, 67, 398, 518, 587, 1368, 1453
MfeI CAATTG 1 cut(s) 324
MflI RGATCY 4 cut(s) 641, 784, 1038, 1373
MhlI GDGCHC 3 cut(s) 40, 1006, 1304
MluCI AATT 9 cut(s) 60, 142, 324, 498, 534, 733, 754, 1420, 1452
MlyI GAGTC 1 cut(s) 1301
MmeI TCCRAC 1 cut(s) 941
MnlI CCTC 7 cut(s) 657, 773, 921, 1006, 1048, 1402, 1513
MroXI GAANNNNTTC 2 cut(s) 744, 1102
MseI TTAA 4 cut(s) 437, 522, 1419, 1512
MslI CAYNNNNRTG 2 cut(s) 698, 884
MspA1I CMGCKG 1 cut(s) 995
MspI CCGG 4 cut(s) 77, 136, 264, 1358
MspR9I CCNGG 4 cut(s) 136, 265, 399, 531
MunI CAATTG 1 cut(s) 324
MvaI CCWGG 2 cut(s) 399, 531
MvnI CGCG 1 cut(s) 1439
MwoI GCNNNNNNNGC 2 cut(s) 1001, 1322
NciI CCSGG 2 cut(s) 136, 265
NcoI CCATGG 1 cut(s) 201
NlaIV GGNNCC 2 cut(s) 249, 1501
NmeAIII GCCGAG 1 cut(s) 1260
NmuCI GTSAC 3 cut(s) 452, 871, 1519
NruI TCGCGA 1 cut(s) 1439
PagI TCATGA 1 cut(s) 729
PciSI GCTCTTC 1 cut(s) 45
PcsI WCGNNNNNNNCGW 1 cut(s) 1478
PdmI GAANNNNTTC 2 cut(s) 744, 1102
PfeI GAWTC 9 cut(s) 347, 409, 715, 745, 799, 893, 1388, 1434, 1469
PfoI TCCNGGA 1 cut(s) 529
PinAI ACCGGT 1 cut(s) 76
PkrI GCNGC 4 cut(s) 997, 1018, 1138, 1324
Ple19I CGATCG 3 cut(s) 807, 1125, 1182
PleI GAGTC 1 cut(s) 1301
PpsI GAGTC 1 cut(s) 1301
PpuMI RGGWCCY 1 cut(s) 248
PshBI ATTAAT 1 cut(s) 1419
Psp124BI GAGCTC 2 cut(s) 40, 1006
Psp1406I AACGTT 2 cut(s) 354, 519
Psp5II RGGWCCY 1 cut(s) 248
Psp6I CCWGG 2 cut(s) 397, 529
PspGI CCWGG 2 cut(s) 397, 529
PspN4I GGNNCC 2 cut(s) 249, 1501
PspPI GGNCC 2 cut(s) 74, 248
PspPPI RGGWCCY 1 cut(s) 248
PsuI RGATCY 4 cut(s) 641, 784, 1038, 1373
PvuI CGATCG 3 cut(s) 807, 1125, 1182
RruI TCGCGA 1 cut(s) 1439
RsaI GTAC 1 cut(s) 67
RsaNI GTAC 1 cut(s) 66
RseI CAYNNNNRTG 2 cut(s) 698, 884
SacI GAGCTC 2 cut(s) 40, 1006
SapI GCTCTTC 1 cut(s) 45
SaqAI TTAA 4 cut(s) 437, 522, 1419, 1512
SatI GCNGC 4 cut(s) 996, 1017, 1137, 1323
Sau96I GGNCC 2 cut(s) 74, 248
SchI GAGTC 1 cut(s) 1301
ScrFI CCNGG 4 cut(s) 136, 265, 399, 531
SduI GDGCHC 3 cut(s) 40, 1006, 1304
SexAI ACCWGGT 1 cut(s) 397
SfaNI GCATC 3 cut(s) 751, 876, 1390
SinI GGWCC 2 cut(s) 74, 248
SmiMI CAYNNNNRTG 2 cut(s) 698, 884
SmlI CTYRAG 2 cut(s) 113, 1275
SmoI CTYRAG 2 cut(s) 113, 1275
Sse9I AATT 9 cut(s) 60, 142, 324, 498, 534, 733, 754, 1420, 1452
SsiI CCGC 6 cut(s) 198, 225, 993, 1200, 1211, 1322
SspMI CTAG 5 cut(s) 503, 777, 921, 1094, 1383
SstI GAGCTC 2 cut(s) 40, 1006
StyD4I CCNGG 4 cut(s) 134, 263, 397, 529
StyI CCWWGG 3 cut(s) 201, 301, 1093
TaaI ACNGT 5 cut(s) 442, 904, 1113, 1346, 1525
TaiI ACGT 6 cut(s) 132, 357, 393, 522, 942, 1333
TaqI TCGA 8 cut(s) 183, 237, 609, 807, 1065, 1125, 1151, 1472
TaqII GACCGA 1 cut(s) 455
TasI AATT 9 cut(s) 60, 142, 324, 498, 534, 733, 754, 1420, 1452
TatI WGTACW 1 cut(s) 65
TauI GCSGC 1 cut(s) 1325
TfiI GAWTC 9 cut(s) 347, 409, 715, 745, 799, 893, 1388, 1434, 1469
Tru1I TTAA 4 cut(s) 437, 522, 1419, 1512
Tru9I TTAA 4 cut(s) 437, 522, 1419, 1512
TscAI CASTG 3 cut(s) 217, 940, 1260
TseFI GTSAC 3 cut(s) 452, 871, 1519
TseI GCWGC 3 cut(s) 995, 1016, 1136
Tsp45I GTSAC 3 cut(s) 452, 871, 1519
TspDTI ATGAA 4 cut(s) 140, 746, 757, 1051
TspGWI ACGGA 1 cut(s) 1421
TspRI CASTG 3 cut(s) 217, 940, 1260
VpaK11BI GGWCC 2 cut(s) 74, 248
VspI ATTAAT 1 cut(s) 1419
XapI RAATTY 2 cut(s) 754, 1452
XmaJI CCTAGG 1 cut(s) 1093
XmnI GAANNNNTTC 2 cut(s) 744, 1102
XspI CTAG 5 cut(s) 503, 777, 921, 1094, 1383
ZraI GACGTC 1 cut(s) 391
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.