FvH4_3g14970

Belongs to the pyruvate kinase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0016515
Physical Location & Seq
Forward (+)
9281764 .. 9284007
2244 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g14970.t1

Sequence Viewer

Length: 1515 bp
ATGGCAATGGAGAAGGTACTTGCTTCTGTGGGAGAGATGAGGCCAAAGACCAAGATTGTGTGCACATTGGGACCGTCTTCCAGGTCCGTGGAGATGGCAGAGAAGCTTTTGAGGGCTGGCATGAACGTTGCTCGCTTCAACTTCTCTCATGGTAGTCATGAGTATCATCAGGAAACTCTTGATAATTTAAGGAGTGCCATGAACAACACCGGAATTCTCTGTGCTGTCATGTTGGATACTAAGGGTCCAGAAATTAGAACTGGGTTTCTGAAAGATGGAAAGGCCATTCATCTCCAACAGGGAAAAGAGATTATCATATCAACTGACTATACCATTCATGGTGATGAGAATATGATCTCTATGAGCTACAAGAAGTTGGCTGAGGATGTGAAGCCTCAAAGTGACATCTTATGTGCTGATGGAACAATTACTTTGAAAGTTCTGGCATGTGACAAGGAGCGTGGTTTGGTACGTTGTCGATGTGAGAACACTGCAGTTTTGGGTGAGAGGAAGAATGTCAATCTTCCTGGAGTTGTGGTTGATCTGCCAACTTTGACAGAGAAGGACAAAGTAGATATATTGGAGTGGGGAGTTTTAAATAAGATTGACATGATCGCGCTCTCTTTTGTTAGGAAAGGCTCAGACCTTGTTGAGGTCAGAAAACTTTTGGGGGAGCATGCCAAGGATATAATGCTCATGTCTAAGGTTGAGAACCAAGAAGGTGTTACTAATTTCGACGACATACTAGCAAATTCAGATGCCTTCATGGTGGCCAGGGGTGACCTTGGGATGGAAATTCCAATTGAAAAGATATTCTTAGCTCAGAAACTGATGATTGCAAAGGCCAACAGACTAGGAAAGCCAGTGGTGACTGCTACTCAGATGCTCGAGTCAATGACCAAATCTCCTCGTCCCACCCGAGCAGAAGCCACTGATGTTGCCAATGCTGTTCTTGATGGAACTGACTGTGTCATGCTCAGCGGCGAAACTGCAGCTGGATCCTATCCGGAAATTGCTGTTCAAACCATGGCTAAAATATGTGTCTCTGCAGAACAGTACATAAACTATGGAGAAATTTTCAAGAAAATGCAATATGAAGCAATGCCTATGAGCCCTCAAGAGAGTCTGGCCTCCTCGGCAGTGCACATGGCAACTTCCATCAGGGCTGCACTCATTTTGGTCCTGACAAGAGGAGGAACCACGGCAAAGCTTGTGGCTAAGTACAGGCCAAGCATGCCGATTTTGTCTGTGGTAGTTCCTGAGATAACTACAGACTCTTTCGAGTGGTCATGCAGCGATGCAGCTCCTGCAAGGCACAGCCTTATTTATAGAGGTCTTGTGCCTCTTCTCAGCTCAGGTTCCATTAAAGGCTCTCATGCTGAGTCTACTGAAGAGGCTGTGCAGTCTGCTATTCAATATGCAAAGGCTAATGGTCTTTGCAAGCCTGGGGATTCAATTGTGGCTCTGCACCGAATGAACTGCACCGACGTCTGCAACATCTTCCCTGTCATATGA

Protein Analysis

505

Amino Acids

54.86

Weight (kDa)

6.25

Isoelectric Point (pI)

34.16

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PK PF00224 16 - 340 4.6e-147 Pyruvate kinase, barrel domain
PK_C PF02887 374 - 494 1.1e-22 Pyruvate kinase, alpha/beta domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016515)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G04050 AT3G25960 AT3G55650
fragaria_vesca FvH4_3g14970
malus_domestica MD10G1210700.v1.1
prunus_persica Prupe.4G134000_v2.0.a1
rosa_chinensis RchiOBHm_Chr5g0024561
rosa_laevigata RLG00000032807
rosa_multiflora Rmu_sc0005699.1_g000007
rosa_rugosa Rorug05G0084000
rosa_samantha Rh5AG175700 Rh5CG189100 Rh5DG174800
rosa_wichuraiana Rw5G015880

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 1491
AccI GTMKAC 1 cut(s) 1385
AccII CGCG 1 cut(s) 617
AccIII TCCGGA 1 cut(s) 1006
AciI CCGC 1 cut(s) 981
AclI AACGTT 1 cut(s) 126
AclWI GGATC 2 cut(s) 993, 1006
AcoI YGGCCR 1 cut(s) 771
AcsI RAATTY 4 cut(s) 213, 751, 795, 1074
AcuI CTGAAG 1 cut(s) 1410
AcyI GRCGYC 1 cut(s) 1488
AfaI GTAC 4 cut(s) 18, 471, 1058, 1223
AfiI CCNNNNNNNGG 2 cut(s) 652, 790
AgsI TTSAA 7 cut(s) 139, 436, 806, 1022, 1081, 1415, 1455
AjnI CCWGG 4 cut(s) 80, 526, 773, 1444
AluBI AGCT 7 cut(s) 106, 366, 821, 995, 1210, 1304, 1353
AluI AGCT 7 cut(s) 106, 366, 821, 995, 1210, 1304, 1353
Alw21I GWGCWC 2 cut(s) 65, 1146
Alw26I GTCTC 1 cut(s) 1048
Alw44I GTGCAC 2 cut(s) 61, 1142
AlwI GGATC 2 cut(s) 993, 1006
AlwNI CAGNNNCTG 2 cut(s) 829, 1307
Ama87I CYCGRG 2 cut(s) 887, 918
Aor13HI TCCGGA 1 cut(s) 1006
AoxI GGCC 6 cut(s) 41, 282, 771, 843, 1128, 1226
ApaLI GTGCAC 2 cut(s) 61, 1142
ApeKI GCWGC 4 cut(s) 992, 1166, 1293, 1301
ApoI RAATTY 4 cut(s) 213, 751, 795, 1074
AspLEI GCGC 1 cut(s) 619
AspS9I GGNCC 4 cut(s) 71, 84, 245, 1180
AsuHPI GGTGA 4 cut(s) 353, 515, 791, 880
AvaI CYCGRG 2 cut(s) 887, 918
AvaII GGWCC 4 cut(s) 71, 84, 245, 1180
BaeGI GKGCMC 2 cut(s) 65, 1146
BalI TGGCCA 1 cut(s) 773
BamHI GGATCC 1 cut(s) 998
BanII GRGCYC 1 cut(s) 1115
BbsI GAAGAC 1 cut(s) 69
Bbv12I GWGCWC 2 cut(s) 65, 1146
BbvCI CCTCAGC 1 cut(s) 381
BbvI GCAGC 4 cut(s) 1004, 1153, 1305, 1313
BccI CCATC 6 cut(s) 88, 269, 413, 784, 950, 1166
BceAI ACGGC 1 cut(s) 1218
BcgI CGANNNNNNTGC 2 cut(s) 1461, 1495
BciT130I CCWGG 4 cut(s) 82, 528, 775, 1446
BciVI GTATCC 1 cut(s) 229
BcoDI GTCTC 1 cut(s) 1048
BfaI CTAG 2 cut(s) 746, 854
BfmI CTRYAG 4 cut(s) 492, 990, 1047, 1269
BfuI GTATCC 1 cut(s) 229
BglI GCCNNNNNGGC 1 cut(s) 1136
BisI GCNGC 5 cut(s) 982, 993, 1167, 1294, 1302
BlpI GCTNAGC 1 cut(s) 977
BlsI GCNGC 5 cut(s) 983, 994, 1168, 1295, 1303
Bme1390I CCNGG 4 cut(s) 82, 528, 775, 1446
Bme18I GGWCC 4 cut(s) 71, 84, 245, 1180
BmeT110I CYCGRG 2 cut(s) 887, 918
BmgT120I GGNCC 4 cut(s) 71, 84, 245, 1180
BmiI GGNNCC 5 cut(s) 72, 246, 1000, 1198, 1360
BmrFI CCNGG 4 cut(s) 82, 528, 775, 1446
BmrI ACTGGG 1 cut(s) 270
BmsI GCATC 3 cut(s) 748, 873, 1288
BmuI ACTGGG 1 cut(s) 270
BpiI GAAGAC 1 cut(s) 69
BpmI CTGGAG 1 cut(s) 549
Bpu10I CCTNAGC 2 cut(s) 381, 1354
Bpu1102I GCTNAGC 1 cut(s) 977
BpuEI CTTGAG 1 cut(s) 1101
BsaHI GRCGYC 1 cut(s) 1488
BsaJI CCNNGG 8 cut(s) 87, 681, 774, 784, 1026, 1134, 1200, 1445
BsaWI WCCGGW 2 cut(s) 209, 1006
BsaXI ACNNNNNCTCC 4 cut(s) 522, 552, 889, 919
Bsc4I CCNNNNNNNGG 2 cut(s) 652, 790
Bse1I ACTGG 2 cut(s) 265, 863
Bse3DI GCAATG 2 cut(s) 12, 1107
BseAI TCCGGA 1 cut(s) 1006
BseBI CCWGG 4 cut(s) 82, 528, 775, 1446
BseDI CCNNGG 8 cut(s) 87, 681, 774, 784, 1026, 1134, 1200, 1445
BseGI GGATG 2 cut(s) 391, 795
BseLI CCNNNNNNNGG 2 cut(s) 652, 790
BseMI GCAATG 2 cut(s) 12, 1107
BseMII CTCAG 9 cut(s) 372, 654, 836, 893, 991, 1251, 1363, 1368, 1371
BseNI ACTGG 2 cut(s) 265, 863
BseRI GAGGAG 3 cut(s) 897, 1123, 1206
BseSI GKGCMC 2 cut(s) 65, 1146
BseXI GCAGC 4 cut(s) 1004, 1153, 1305, 1313
BsgI GTGCAG 4 cut(s) 1152, 1421, 1451, 1465
Bsh1236I CGCG 1 cut(s) 617
BshFI GGCC 6 cut(s) 43, 284, 773, 845, 1130, 1228
BsiHKAI GWGCWC 2 cut(s) 65, 1146
BsiHKCI CYCGRG 2 cut(s) 887, 918
BsiSI CCGG 2 cut(s) 210, 1007
BslFI GGGAC 2 cut(s) 84, 897
BslI CCNNNNNNNGG 2 cut(s) 652, 790
BsmAI GTCTC 1 cut(s) 1048
BsmFI GGGAC 2 cut(s) 84, 897
BsnI GGCC 6 cut(s) 43, 284, 773, 845, 1130, 1228
BsoBI CYCGRG 2 cut(s) 887, 918
Bsp1286I GDGCHC 3 cut(s) 65, 1115, 1146
Bsp13I TCCGGA 1 cut(s) 1006
Bsp143I GATC 4 cut(s) 354, 541, 612, 998
Bsp1720I GCTNAGC 1 cut(s) 977
Bsp19I CCATGG 1 cut(s) 1026
BspACI CCGC 1 cut(s) 981
BspANI GGCC 6 cut(s) 43, 284, 773, 845, 1130, 1228
BspCNI CTCAG 9 cut(s) 373, 653, 835, 892, 990, 1252, 1362, 1367, 1372
BspEI TCCGGA 1 cut(s) 1006
BspFNI CGCG 1 cut(s) 617
BspHI TCATGA 1 cut(s) 157
BspLI GGNNCC 5 cut(s) 72, 246, 1000, 1198, 1360
BspMAI CTGCAG 3 cut(s) 496, 994, 1051
BspPI GGATC 2 cut(s) 993, 1006
BsrDI GCAATG 2 cut(s) 12, 1107
BsrI ACTGG 2 cut(s) 265, 863
BssECI CCNNGG 8 cut(s) 87, 681, 774, 784, 1026, 1134, 1200, 1445
BssMI GATC 4 cut(s) 354, 541, 612, 998
BssNI GRCGYC 1 cut(s) 1488
BssT1I CCWWGG 3 cut(s) 681, 784, 1026
Bst2UI CCWGG 4 cut(s) 82, 528, 775, 1446
Bst4CI ACNGT 3 cut(s) 75, 968, 1056
Bst6I CTCTTC 2 cut(s) 1350, 1386
BstACI GRCGYC 1 cut(s) 1488
BstAPI GCANNNNNTGC 1 cut(s) 1307
BstC8I GCNNGC 5 cut(s) 118, 133, 678, 1235, 1442
BstDSI CCRYGG 3 cut(s) 87, 1026, 1200
BstEII GGTNACC 1 cut(s) 779
BstENI CCTNNNNNAGG 1 cut(s) 650
BstF5I GGATG 2 cut(s) 391, 795
BstFNI CGCG 1 cut(s) 617
BstHHI GCGC 1 cut(s) 619
BstKTI GATC 4 cut(s) 357, 544, 615, 1001
BstMAI GTCTC 1 cut(s) 1048
BstMBI GATC 4 cut(s) 354, 541, 612, 998
BstMWI GCNNNNNNNGC 3 cut(s) 1136, 1234, 1307
BstNI CCWGG 4 cut(s) 82, 528, 775, 1446
BstNSI RCATGY 3 cut(s) 450, 680, 1237
BstPI GGTNACC 1 cut(s) 779
BstSCI CCNGG 4 cut(s) 80, 526, 773, 1444
BstSFI CTRYAG 4 cut(s) 492, 990, 1047, 1269
BstSLI GKGCMC 2 cut(s) 65, 1146
BstUI CGCG 1 cut(s) 617
BstV1I GCAGC 4 cut(s) 1004, 1153, 1305, 1313
BstV2I GAAGAC 1 cut(s) 69
BstX2I RGATCY 1 cut(s) 998
BstXI CCANNNNNNTGG 1 cut(s) 88
BstYI RGATCY 1 cut(s) 998
BsuI GTATCC 1 cut(s) 229
BsuRI GGCC 6 cut(s) 43, 284, 773, 845, 1130, 1228
BtgI CCRYGG 3 cut(s) 87, 1026, 1200
BtgZI GCGATG 1 cut(s) 1311
BtsCI GGATG 2 cut(s) 391, 795
BtsI GCAGTG 2 cut(s) 489, 1146
BtsIMutI CAGTG 4 cut(s) 489, 870, 930, 1146
Cac8I GCNNGC 5 cut(s) 118, 133, 678, 1235, 1442
CaiI CAGNNNCTG 2 cut(s) 829, 1307
CciI TCATGA 1 cut(s) 157
CfoI GCGC 1 cut(s) 619
Cfr13I GGNCC 4 cut(s) 71, 84, 245, 1180
Csp6I GTAC 4 cut(s) 17, 470, 1057, 1222
CspCI CAANNNNNGTGG 6 cut(s) 442, 477, 919, 954, 1194, 1229
CviQI GTAC 4 cut(s) 17, 470, 1057, 1222
DpnI GATC 4 cut(s) 356, 543, 614, 1000
DpnII GATC 4 cut(s) 354, 541, 612, 998
DraI TTTAAA 1 cut(s) 597
EaeI YGGCCR 1 cut(s) 771
Eam1104I CTCTTC 2 cut(s) 1350, 1386
EarI CTCTTC 2 cut(s) 1350, 1386
Eco130I CCWWGG 3 cut(s) 681, 784, 1026
Eco24I GRGCYC 1 cut(s) 1115
Eco47I GGWCC 4 cut(s) 71, 84, 245, 1180
Eco57I CTGAAG 1 cut(s) 1410
Eco88I CYCGRG 2 cut(s) 887, 918
Eco91I GGTNACC 1 cut(s) 779
EcoNI CCTNNNNNAGG 1 cut(s) 650
EcoO65I GGTNACC 1 cut(s) 779
EcoRI GAATTC 1 cut(s) 213
EcoRII CCWGG 4 cut(s) 80, 526, 773, 1444
EcoT14I CCWWGG 3 cut(s) 681, 784, 1026
EcoT38I GRGCYC 1 cut(s) 1115
ErhI CCWWGG 3 cut(s) 681, 784, 1026
FalI AAGNNNNNCTT 2 cut(s) 800, 832
FaqI GGGAC 2 cut(s) 84, 897
FauNDI CATATG 1 cut(s) 1511
FblI GTMKAC 1 cut(s) 1385
Fnu4HI GCNGC 5 cut(s) 982, 993, 1167, 1294, 1302
FokI GGATG 2 cut(s) 398, 802
FriOI GRGCYC 1 cut(s) 1115
Fsp4HI GCNGC 5 cut(s) 982, 993, 1167, 1294, 1302
FspBI CTAG 2 cut(s) 746, 854
GlaI GCGC 1 cut(s) 618
GluI GCNGC 5 cut(s) 982, 993, 1167, 1294, 1302
GsuI CTGGAG 1 cut(s) 549
HaeIII GGCC 6 cut(s) 43, 284, 773, 845, 1130, 1228
HapII CCGG 2 cut(s) 210, 1007
HhaI GCGC 1 cut(s) 619
Hin1I GRCGYC 1 cut(s) 1488
Hin6I GCGC 1 cut(s) 617
HinP1I GCGC 1 cut(s) 617
HindIII AAGCTT 2 cut(s) 104, 1208
HinfI GANTC 5 cut(s) 890, 1123, 1274, 1382, 1451
HpaII CCGG 2 cut(s) 210, 1007
HphI GGTGA 4 cut(s) 353, 515, 791, 880
Hpy166II GTNNAC 3 cut(s) 63, 1144, 1386
Hpy188I TCNGA 6 cut(s) 270, 643, 659, 757, 825, 882
Hpy8I GTNNAC 3 cut(s) 63, 1144, 1386
Hpy99I CGWCG 2 cut(s) 740, 1490
HpyAV CCTTC 4 cut(s) 7, 556, 713, 772
HpyCH4III ACNGT 3 cut(s) 75, 968, 1056
HpyCH4IV ACGT 3 cut(s) 126, 472, 1488
HpyF10VI GCNNNNNNNGC 3 cut(s) 1136, 1234, 1307
HpySE526I ACGT 3 cut(s) 126, 472, 1488
Hsp92I GRCGYC 1 cut(s) 1488
HspAI GCGC 1 cut(s) 617
Kpn2I TCCGGA 1 cut(s) 1006
Kzo9I GATC 4 cut(s) 354, 541, 612, 998
LmnI GCTCC 3 cut(s) 457, 673, 1309
Lsp1109I GCAGC 4 cut(s) 1004, 1153, 1305, 1313
LweI GCATC 3 cut(s) 748, 873, 1288
MaeI CTAG 2 cut(s) 746, 854
MaeII ACGT 3 cut(s) 126, 472, 1488
MaeIII GTNAC 5 cut(s) 401, 449, 724, 779, 868
MalI GATC 4 cut(s) 356, 543, 614, 1000
MboI GATC 4 cut(s) 354, 541, 612, 998
MboII GAAGA 6 cut(s) 69, 515, 523, 1337, 1403, 1492
MfeI CAATTG 2 cut(s) 801, 1455
MflI RGATCY 1 cut(s) 998
MhlI GDGCHC 3 cut(s) 65, 1115, 1146
MlsI TGGCCA 1 cut(s) 773
MluNI TGGCCA 1 cut(s) 773
MlyI GAGTC 4 cut(s) 899, 1132, 1268, 1391
MmeI TCCRAC 2 cut(s) 213, 319
Mox20I TGGCCA 1 cut(s) 773
MroI TCCGGA 1 cut(s) 1006
MscI TGGCCA 1 cut(s) 773
MseI TTAA 3 cut(s) 188, 596, 1365
MslI CAYNNNNRTG 1 cut(s) 342
Msp20I TGGCCA 1 cut(s) 773
MspA1I CMGCKG 2 cut(s) 981, 995
MspI CCGG 2 cut(s) 210, 1007
MspR9I CCNGG 4 cut(s) 82, 528, 775, 1446
MunI CAATTG 2 cut(s) 801, 1455
MvaI CCWGG 4 cut(s) 82, 528, 775, 1446
MvnI CGCG 1 cut(s) 617
MwoI GCNNNNNNNGC 3 cut(s) 1136, 1234, 1307
NcoI CCATGG 1 cut(s) 1026
NdeI CATATG 1 cut(s) 1511
NdeII GATC 4 cut(s) 354, 541, 612, 998
NlaIV GGNNCC 5 cut(s) 72, 246, 1000, 1198, 1360
NmeAIII GCCGAG 1 cut(s) 1115
NmuCI GTSAC 4 cut(s) 401, 449, 779, 868
NspI RCATGY 3 cut(s) 450, 680, 1237
PaeI GCATGC 2 cut(s) 680, 1237
PaeR7I CTCGAG 1 cut(s) 887
PagI TCATGA 1 cut(s) 157
PcsI WCGNNNNNNNCGW 1 cut(s) 916
PfeI GAWTC 1 cut(s) 1451
PflFI GACNNNGTC 1 cut(s) 968
PfoI TCCNGGA 1 cut(s) 526
PkrI GCNGC 5 cut(s) 983, 994, 1168, 1295, 1303
PleI GAGTC 4 cut(s) 898, 1131, 1268, 1390
PpsI GAGTC 4 cut(s) 898, 1131, 1268, 1390
Psp1406I AACGTT 1 cut(s) 126
Psp6I CCWGG 4 cut(s) 80, 526, 773, 1444
PspEI GGTNACC 1 cut(s) 779
PspGI CCWGG 4 cut(s) 80, 526, 773, 1444
PspN4I GGNNCC 5 cut(s) 72, 246, 1000, 1198, 1360
PspPI GGNCC 4 cut(s) 71, 84, 245, 1180
PspXI VCTCGAGB 1 cut(s) 887
PstI CTGCAG 3 cut(s) 496, 994, 1051
PstNI CAGNNNCTG 2 cut(s) 829, 1307
PsuI RGATCY 1 cut(s) 998
PsyI GACNNNGTC 1 cut(s) 968
PvuII CAGCTG 1 cut(s) 995
RsaI GTAC 4 cut(s) 18, 471, 1058, 1223
RsaNI GTAC 4 cut(s) 17, 470, 1057, 1222
RseI CAYNNNNRTG 1 cut(s) 342
SaqAI TTAA 3 cut(s) 188, 596, 1365
SatI GCNGC 5 cut(s) 982, 993, 1167, 1294, 1302
Sau3AI GATC 4 cut(s) 354, 541, 612, 998
Sau96I GGNCC 4 cut(s) 71, 84, 245, 1180
SchI GAGTC 4 cut(s) 899, 1132, 1268, 1391
ScrFI CCNGG 4 cut(s) 82, 528, 775, 1446
SduI GDGCHC 3 cut(s) 65, 1115, 1146
SfaNI GCATC 3 cut(s) 748, 873, 1288
SfcI CTRYAG 4 cut(s) 492, 990, 1047, 1269
Sfr274I CTCGAG 1 cut(s) 887
SinI GGWCC 4 cut(s) 71, 84, 245, 1180
SlaI CTCGAG 1 cut(s) 887
SmiMI CAYNNNNRTG 1 cut(s) 342
SmlI CTYRAG 2 cut(s) 887, 1116
SmoI CTYRAG 2 cut(s) 887, 1116
SphI GCATGC 2 cut(s) 680, 1237
SsiI CCGC 1 cut(s) 981
SspMI CTAG 2 cut(s) 746, 854
StyD4I CCNGG 4 cut(s) 80, 526, 773, 1444
StyI CCWWGG 3 cut(s) 681, 784, 1026
TaaI ACNGT 3 cut(s) 75, 968, 1056
TaiI ACGT 3 cut(s) 129, 475, 1491
TaqI TCGA 4 cut(s) 478, 735, 888, 1281
TatI WGTACW 2 cut(s) 1056, 1221
TauI GCSGC 1 cut(s) 984
TfiI GAWTC 1 cut(s) 1451
Tru1I TTAA 3 cut(s) 188, 596, 1365
Tru9I TTAA 3 cut(s) 188, 596, 1365
TscAI CASTG 4 cut(s) 496, 870, 937, 1146
TseFI GTSAC 4 cut(s) 401, 449, 779, 868
TseI GCWGC 4 cut(s) 992, 1166, 1293, 1301
Tsp45I GTSAC 4 cut(s) 401, 449, 779, 868
TspDTI ATGAA 7 cut(s) 137, 215, 278, 326, 754, 1110, 1490
TspGWI ACGGA 1 cut(s) 76
TspRI CASTG 4 cut(s) 496, 870, 937, 1146
Tth111I GACNNNGTC 1 cut(s) 968
VneI GTGCAC 2 cut(s) 61, 1142
VpaK11BI GGWCC 4 cut(s) 71, 84, 245, 1180
XagI CCTNNNNNAGG 1 cut(s) 650
XapI RAATTY 4 cut(s) 213, 751, 795, 1074
XceI RCATGY 3 cut(s) 450, 680, 1237
XhoI CTCGAG 1 cut(s) 887
XmiI GTMKAC 1 cut(s) 1385
XspI CTAG 2 cut(s) 746, 854
ZraI GACGTC 1 cut(s) 1489
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.