MD10G1210700.v1.1

Belongs to the pyruvate kinase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0016515
Physical Location & Seq
Forward (+)
31012673 .. 31014442
1770 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1210700.v1.1.491

Sequence Viewer

Length: 1515 bp
ATGGCAATGGAGAAGGTGCTTGAAGTTGTGGGGGATAGAAAGCCTAAGACTAAGATCGTGTGCACGTTGGGGCCGTCTTCGCGGTCCGTTGAGATGCTGGAGAAGCTTCTGAGGGCCGGGATGAACGTGGCTCGCTTCAACTTCTCACATGGTACTCATGCTTACCATCAAGAGACTCTGGATAATCTTAGGACCGCCATGAACAACACCGGCATCCTCTGCGCCGTCATGTTGGACACTAAGGGTCCAGAAATTCGAACGGGATTTCTGAAAGACGGGAAGTCTATAAATCTTAAACAAGGTCAAGAGATCACCATCACCACTGACTATAACATTCTTGGTGATGAGAGTTTGATCTGTATGAGCTACAAAAGGTTGGCTGAGGATGTGAAACCGCAAACCGATATCTTGTGTGCTGATGGAAAAATTACTCTGAGGGTTCTGGCTTGCGACGAGGAGCGTGGTTTGGTGCATTGTCGCTGTGAAAACTCTGCAGAGCTGGGTGAGAAGAAGAATGTCAATCTCCCTGGAGTGGTTGTTGATCTGCCAACACTAACAGAGAAGGACAAAGTTGATATCTTGGAGTGGGGGGTTCCCAATAAGATTGACATGATCGCACTCTCTTTCGTGCGCAAAGGTTCAGACCTTCTTGAGGTCAGAAAATTGTTGGGGGAGCATGCAAAGAATATAATGCTCATGTCCAAGGTTGAGAATCAAGAAGGGGTTTCCAACTTTGATGAAATTCTTGCAAATTCGGATGCGTTTATGGTGGCGAGGGGTGACCTCGGAATGGAGATTCCAATTGAGAAGATATTCTTAGCTCAGAAATTAATGATAGAGAAGGCCAACAGACTAGGAAAGCCGATCGTGACTGCTACTCAGCTGTTAGAATCCATGGTCAAGTCTCCTCGTCCCACACGAGCTGAAGCCACCGATGTTGCCAATGCAGTTCTTGACGGAACTGACTGTGTGATGCTCAGCGGTGAAACAGCAGCGGGAGCCTATCCAGAAATTGCAGTTCAAACCATGGCCAAAATCTGTGTTTCTGCTGAGGACTCTATAAATTATGGGGAGCTTTTCAAAAGAAAGATGGAAGTTGCAGCAATGCCTATGTCCCCACTAGAGAGCTTGGCCTCCTCAGCAGTTCAGACAGCGAATTGCATCAAGGCGGCACTGATTTTAGTCCTCACCAAAGGAGGCAGCACGGCTAAGCTGGTGGCTAAGTACAGGCCAAGTATGCCGATTTTGTCTGTGGTGGTGCCTGAGATAACAACAGACTCTTTCGAGTGGTCTTGCAGCGATGCAGCTCCAGCTAGGCACGGCCTTATTTACCAGGGAGTTCTGCCTCTTCTCAGCTCAAGTCCCATTAGGGCTTCTCATGCTGAGTCCACCGAAGAGAGCATACATTTTGCCATTCAACATGCAAAAGCAAAGGGACTTTGCAAGCCTGGAGACTCCGTTGTGGCGTTGCACCGCGTTAACACTGATATGATCAAGATCTTGACTGTGAAGTGA

Protein Analysis

505

Amino Acids

54.84

Weight (kDa)

6.26

Isoelectric Point (pI)

37.41

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PK PF00224 16 - 340 2.7e-144 Pyruvate kinase, barrel domain
PK_C PF02887 375 - 500 1.4e-23 Pyruvate kinase, alpha/beta domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016515)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G04050 AT3G25960 AT3G55650
fragaria_vesca FvH4_3g14970
malus_domestica MD10G1210700.v1.1
prunus_persica Prupe.4G134000_v2.0.a1
rosa_chinensis RchiOBHm_Chr5g0024561
rosa_laevigata RLG00000032807
rosa_multiflora Rmu_sc0005699.1_g000007
rosa_rugosa Rorug05G0084000
rosa_samantha Rh5AG175700 Rh5CG189100 Rh5DG174800
rosa_wichuraiana Rw5G015880

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 632
AccB1I GGYRCC 1 cut(s) 1258
AccII CGCG 2 cut(s) 82, 1476
AciI CCGC 7 cut(s) 82, 195, 395, 981, 995, 1169, 1474
AcoI YGGCCR 1 cut(s) 1029
AcsI RAATTY 3 cut(s) 252, 741, 751
AcuI CTGAAG 1 cut(s) 945
AfaI GTAC 2 cut(s) 154, 1226
AfiI CCNNNNNNNGG 3 cut(s) 532, 652, 790
AgsI TTSAA 5 cut(s) 23, 139, 1022, 1081, 1418
AhdI GACNNNNNGTC 1 cut(s) 280
AjnI CCWGG 3 cut(s) 526, 1332, 1447
Alw21I GWGCWC 1 cut(s) 65
Alw26I GTCTC 3 cut(s) 167, 909, 1446
Alw44I GTGCAC 1 cut(s) 61
AoxI GGCC 7 cut(s) 71, 114, 843, 1029, 1131, 1229, 1321
ApaLI GTGCAC 1 cut(s) 61
ApeKI GCWGC 5 cut(s) 992, 1100, 1200, 1296, 1304
ApoI RAATTY 3 cut(s) 252, 741, 751
AseI ATTAAT 1 cut(s) 830
Asp700I GAANNNNTTC 1 cut(s) 812
AspLEI GCGC 2 cut(s) 224, 633
AspS9I GGNCC 5 cut(s) 71, 84, 114, 192, 245
AsuC2I CCSGG 1 cut(s) 118
AsuHPI GGTGA 7 cut(s) 304, 310, 353, 515, 791, 995, 1180
AsuII TTCGAA 1 cut(s) 256
AvaII GGWCC 3 cut(s) 84, 192, 245
BaeGI GKGCMC 1 cut(s) 65
BalI TGGCCA 1 cut(s) 1031
BanI GGYRCC 1 cut(s) 1258
BarI GAAGNNNNNNTAC 2 cut(s) 1386, 1418
BauI CACGAG 1 cut(s) 918
BbsI GAAGAC 1 cut(s) 69
Bbv12I GWGCWC 1 cut(s) 65
BbvCI CCTCAGC 3 cut(s) 381, 1050, 1138
BbvI GCAGC 5 cut(s) 1004, 1112, 1212, 1308, 1316
BccI CCATC 4 cut(s) 174, 323, 413, 1084
BceAI ACGGC 4 cut(s) 58, 209, 1221, 1336
BciT130I CCWGG 3 cut(s) 528, 1334, 1449
BclI TGATCA 1 cut(s) 1491
BcnI CCSGG 1 cut(s) 118
BcoDI GTCTC 3 cut(s) 167, 909, 1446
BfaI CTAG 3 cut(s) 854, 1121, 1314
BfmI CTRYAG 1 cut(s) 492
BglII AGATCT 1 cut(s) 1497
BisI GCNGC 6 cut(s) 993, 1101, 1170, 1201, 1297, 1305
BlpI GCTNAGC 2 cut(s) 977, 1209
BlsI GCNGC 6 cut(s) 994, 1102, 1171, 1202, 1298, 1306
Bme1390I CCNGG 4 cut(s) 118, 528, 1334, 1449
Bme18I GGWCC 3 cut(s) 84, 192, 245
BmeRI GACNNNNNGTC 1 cut(s) 280
BmgT120I GGNCC 5 cut(s) 71, 84, 114, 192, 245
BmiI GGNNCC 5 cut(s) 72, 246, 594, 1000, 1260
BmrFI CCNGG 4 cut(s) 118, 528, 1334, 1449
BmsI GCATC 6 cut(s) 84, 222, 748, 963, 1170, 1291
BpiI GAAGAC 1 cut(s) 69
BpmI CTGGAG 4 cut(s) 119, 549, 1293, 1470
Bpu10I CCTNAGC 3 cut(s) 381, 1050, 1138
Bpu1102I GCTNAGC 2 cut(s) 977, 1209
Bpu14I TTCGAA 1 cut(s) 256
BpuEI CTTGAG 2 cut(s) 671, 1342
BpuMI CCSGG 1 cut(s) 118
BsaBI GATNNNNATC 2 cut(s) 314, 1496
BsaJI CCNNGG 6 cut(s) 526, 702, 784, 894, 1026, 1333
BsaXI ACNNNNNCTCC 4 cut(s) 522, 552, 1443, 1473
Bsc4I CCNNNNNNNGG 3 cut(s) 532, 652, 790
Bse118I RCCGGY 1 cut(s) 209
Bse3DI GCAATG 2 cut(s) 12, 1110
Bse8I GATNNNNATC 2 cut(s) 314, 1496
BseBI CCWGG 3 cut(s) 528, 1334, 1449
BseDI CCNNGG 6 cut(s) 526, 702, 784, 894, 1026, 1333
BseGI GGATG 4 cut(s) 126, 213, 391, 763
BseJI GATNNNNATC 2 cut(s) 314, 1496
BseLI CCNNNNNNNGG 3 cut(s) 532, 652, 790
BseMI GCAATG 2 cut(s) 12, 1110
BseRI GAGGAG 3 cut(s) 470, 897, 1126
BseSI GKGCMC 1 cut(s) 65
BseXI GCAGC 5 cut(s) 1004, 1112, 1212, 1308, 1316
BseYI CCCAGC 1 cut(s) 499
Bsh1236I CGCG 2 cut(s) 82, 1476
Bsh1285I CGRYCG 1 cut(s) 867
BshFI GGCC 7 cut(s) 73, 116, 845, 1031, 1133, 1231, 1323
BshNI GGYRCC 1 cut(s) 1258
BsiEI CGRYCG 1 cut(s) 867
BsiHKAI GWGCWC 1 cut(s) 65
BsiSI CCGG 2 cut(s) 117, 210
BslFI GGGAC 4 cut(s) 897, 1099, 1347, 1449
BslI CCNNNNNNNGG 3 cut(s) 532, 652, 790
BsmAI GTCTC 3 cut(s) 167, 909, 1446
BsmFI GGGAC 4 cut(s) 897, 1099, 1347, 1449
BsnI GGCC 7 cut(s) 73, 116, 845, 1031, 1133, 1231, 1323
Bsp119I TTCGAA 1 cut(s) 256
Bsp1286I GDGCHC 1 cut(s) 65
Bsp143I GATC 8 cut(s) 54, 309, 354, 541, 612, 864, 1491, 1497
Bsp1720I GCTNAGC 2 cut(s) 977, 1209
Bsp19I CCATGG 2 cut(s) 894, 1026
BspACI CCGC 7 cut(s) 82, 195, 395, 981, 995, 1169, 1474
BspANI GGCC 7 cut(s) 73, 116, 845, 1031, 1133, 1231, 1323
BspFNI CGCG 2 cut(s) 82, 1476
BspLI GGNNCC 5 cut(s) 72, 246, 594, 1000, 1260
BspMAI CTGCAG 1 cut(s) 496
BspT104I TTCGAA 1 cut(s) 256
BspT107I GGYRCC 1 cut(s) 1258
BsrDI GCAATG 2 cut(s) 12, 1110
BsrFI RCCGGY 1 cut(s) 209
BssAI RCCGGY 1 cut(s) 209
BssECI CCNNGG 6 cut(s) 526, 702, 784, 894, 1026, 1333
BssMI GATC 8 cut(s) 54, 309, 354, 541, 612, 864, 1491, 1497
BssSI CACGAG 1 cut(s) 918
BssT1I CCWWGG 3 cut(s) 702, 894, 1026
Bst2BI CACGAG 1 cut(s) 918
Bst2UI CCWGG 3 cut(s) 528, 1334, 1449
Bst4CI ACNGT 2 cut(s) 968, 1507
Bst6I CTCTTC 2 cut(s) 1353, 1389
BstAPI GCANNNNNTGC 1 cut(s) 219
BstBI TTCGAA 1 cut(s) 256
BstC8I GCNNGC 4 cut(s) 133, 448, 678, 1445
BstDSI CCRYGG 2 cut(s) 894, 1026
BstEII GGTNACC 1 cut(s) 779
BstENI CCTNNNNNAGG 1 cut(s) 650
BstF5I GGATG 4 cut(s) 126, 213, 391, 763
BstFNI CGCG 2 cut(s) 82, 1476
BstHHI GCGC 2 cut(s) 224, 633
BstKTI GATC 8 cut(s) 57, 312, 357, 544, 615, 867, 1494, 1500
BstMAI GTCTC 3 cut(s) 167, 909, 1446
BstMBI GATC 8 cut(s) 54, 309, 354, 541, 612, 864, 1491, 1497
BstMCI CGRYCG 1 cut(s) 867
BstMWI GCNNNNNNNGC 8 cut(s) 79, 103, 219, 998, 1139, 1237, 1310, 1379
BstNI CCWGG 3 cut(s) 528, 1334, 1449
BstNSI RCATGY 2 cut(s) 680, 1424
BstPI GGTNACC 1 cut(s) 779
BstSCI CCNGG 4 cut(s) 116, 526, 1332, 1447
BstSFI CTRYAG 1 cut(s) 492
BstSLI GKGCMC 1 cut(s) 65
BstUI CGCG 2 cut(s) 82, 1476
BstV1I GCAGC 5 cut(s) 1004, 1112, 1212, 1308, 1316
BstV2I GAAGAC 1 cut(s) 69
BstX2I RGATCY 1 cut(s) 1497
BstYI RGATCY 1 cut(s) 1497
BsuRI GGCC 7 cut(s) 73, 116, 845, 1031, 1133, 1231, 1323
BtgI CCRYGG 2 cut(s) 894, 1026
BtgZI GCGATG 1 cut(s) 1314
BtsCI GGATG 4 cut(s) 126, 213, 391, 763
BtsIMutI CAGTG 3 cut(s) 321, 1172, 1482
Cac8I GCNNGC 4 cut(s) 133, 448, 678, 1445
CfoI GCGC 2 cut(s) 224, 633
Cfr10I RCCGGY 1 cut(s) 209
Cfr13I GGNCC 5 cut(s) 71, 84, 114, 192, 245
CpoI CGGWCCG 1 cut(s) 84
Csp6I GTAC 2 cut(s) 153, 1225
CspCI CAANNNNNGTGG 2 cut(s) 919, 954
CspI CGGWCCG 1 cut(s) 84
CviQI GTAC 2 cut(s) 153, 1225
DpnI GATC 8 cut(s) 56, 311, 356, 543, 614, 866, 1493, 1499
DpnII GATC 8 cut(s) 54, 309, 354, 541, 612, 864, 1491, 1497
DriI GACNNNNNGTC 1 cut(s) 280
EaeI YGGCCR 1 cut(s) 1029
Eam1104I CTCTTC 2 cut(s) 1353, 1389
Eam1105I GACNNNNNGTC 1 cut(s) 280
EarI CTCTTC 2 cut(s) 1353, 1389
Eco130I CCWWGG 3 cut(s) 702, 894, 1026
Eco32I GATATC 2 cut(s) 406, 577
Eco47I GGWCC 3 cut(s) 84, 192, 245
Eco57I CTGAAG 1 cut(s) 945
Eco91I GGTNACC 1 cut(s) 779
EcoNI CCTNNNNNAGG 1 cut(s) 650
EcoO65I GGTNACC 1 cut(s) 779
EcoRII CCWGG 3 cut(s) 526, 1332, 1447
EcoRV GATATC 2 cut(s) 406, 577
EcoT14I CCWWGG 3 cut(s) 702, 894, 1026
ErhI CCWWGG 3 cut(s) 702, 894, 1026
FalI AAGNNNNNCTT 2 cut(s) 800, 832
FaqI GGGAC 4 cut(s) 897, 1099, 1347, 1449
FauI CCCGC 1 cut(s) 988
FbaI TGATCA 1 cut(s) 1491
Fnu4HI GCNGC 6 cut(s) 993, 1101, 1170, 1201, 1297, 1305
FokI GGATG 4 cut(s) 133, 200, 398, 770
Fsp4HI GCNGC 6 cut(s) 993, 1101, 1170, 1201, 1297, 1305
FspBI CTAG 3 cut(s) 854, 1121, 1314
FspI TGCGCA 1 cut(s) 632
GlaI GCGC 2 cut(s) 223, 632
GluI GCNGC 6 cut(s) 993, 1101, 1170, 1201, 1297, 1305
GsaI CCCAGC 1 cut(s) 503
GsuI CTGGAG 4 cut(s) 119, 549, 1293, 1470
HaeIII GGCC 7 cut(s) 73, 116, 845, 1031, 1133, 1231, 1323
HapII CCGG 2 cut(s) 117, 210
HhaI GCGC 2 cut(s) 224, 633
Hin6I GCGC 2 cut(s) 222, 631
HinP1I GCGC 2 cut(s) 222, 631
HincII GTYRAC 1 cut(s) 1480
HindII GTYRAC 1 cut(s) 1480
HindIII AAGCTT 1 cut(s) 104
HinfI GANTC 8 cut(s) 175, 712, 796, 890, 1055, 1277, 1385, 1454
HpaI GTTAAC 1 cut(s) 1480
HpaII CCGG 2 cut(s) 117, 210
HphI GGTGA 7 cut(s) 304, 310, 353, 515, 791, 995, 1180
Hpy166II GTNNAC 3 cut(s) 63, 1389, 1480
Hpy188I TCNGA 9 cut(s) 111, 270, 435, 643, 659, 757, 788, 825, 1149
Hpy8I GTNNAC 3 cut(s) 63, 1389, 1480
Hpy99I CGWCG 1 cut(s) 455
HpyAV CCTTC 5 cut(s) 7, 556, 656, 713, 835
HpyCH4III ACNGT 2 cut(s) 968, 1507
HpyCH4IV ACGT 2 cut(s) 65, 126
HpyF10VI GCNNNNNNNGC 8 cut(s) 79, 103, 219, 998, 1139, 1237, 1310, 1379
HpySE526I ACGT 2 cut(s) 65, 126
HspAI GCGC 2 cut(s) 222, 631
Ksp22I TGATCA 1 cut(s) 1491
KspAI GTTAAC 1 cut(s) 1480
Kzo9I GATC 8 cut(s) 54, 309, 354, 541, 612, 864, 1491, 1497
LmnI GCTCC 5 cut(s) 457, 673, 998, 1072, 1312
Lsp1109I GCAGC 5 cut(s) 1004, 1112, 1212, 1308, 1316
LweI GCATC 6 cut(s) 84, 222, 748, 963, 1170, 1291
MaeI CTAG 3 cut(s) 854, 1121, 1314
MaeII ACGT 2 cut(s) 65, 126
MaeIII GTNAC 2 cut(s) 779, 868
MalI GATC 8 cut(s) 56, 311, 356, 543, 614, 866, 1493, 1499
MboI GATC 8 cut(s) 54, 309, 354, 541, 612, 864, 1491, 1497
MboII GAAGA 6 cut(s) 69, 520, 523, 820, 1340, 1406
MfeI CAATTG 1 cut(s) 801
MflI RGATCY 1 cut(s) 1497
MhlI GDGCHC 1 cut(s) 65
MlsI TGGCCA 1 cut(s) 1031
MluNI TGGCCA 1 cut(s) 1031
MlyI GAGTC 5 cut(s) 169, 1049, 1271, 1394, 1448
MmeI TCCRAC 2 cut(s) 213, 753
Mox20I TGGCCA 1 cut(s) 1031
MroXI GAANNNNTTC 1 cut(s) 812
MscI TGGCCA 1 cut(s) 1031
MseI TTAA 3 cut(s) 294, 830, 1479
MslI CAYNNNNRTG 1 cut(s) 1487
Msp20I TGGCCA 1 cut(s) 1031
MspA1I CMGCKG 3 cut(s) 883, 981, 995
MspI CCGG 2 cut(s) 117, 210
MspR9I CCNGG 4 cut(s) 118, 528, 1334, 1449
MunI CAATTG 1 cut(s) 801
MvaI CCWGG 3 cut(s) 528, 1334, 1449
MvnI CGCG 2 cut(s) 82, 1476
MwoI GCNNNNNNNGC 8 cut(s) 79, 103, 219, 998, 1139, 1237, 1310, 1379
NciI CCSGG 1 cut(s) 118
NcoI CCATGG 2 cut(s) 894, 1026
NdeII GATC 8 cut(s) 54, 309, 354, 541, 612, 864, 1491, 1497
NlaIV GGNNCC 5 cut(s) 72, 246, 594, 1000, 1260
NmuCI GTSAC 2 cut(s) 779, 868
NsbI TGCGCA 1 cut(s) 632
NspI RCATGY 2 cut(s) 680, 1424
NspV TTCGAA 1 cut(s) 256
PaeI GCATGC 1 cut(s) 680
PcsI WCGNNNNNNNCGW 2 cut(s) 71, 916
PdmI GAANNNNTTC 1 cut(s) 812
PfeI GAWTC 3 cut(s) 712, 796, 890
PkrI GCNGC 6 cut(s) 994, 1102, 1171, 1202, 1298, 1306
Ple19I CGATCG 1 cut(s) 867
PleI GAGTC 5 cut(s) 169, 1049, 1271, 1393, 1448
PpsI GAGTC 5 cut(s) 169, 1049, 1271, 1393, 1448
PshBI ATTAAT 1 cut(s) 830
Psp6I CCWGG 3 cut(s) 526, 1332, 1447
PspEI GGTNACC 1 cut(s) 779
PspFI CCCAGC 1 cut(s) 499
PspGI CCWGG 3 cut(s) 526, 1332, 1447
PspN4I GGNNCC 5 cut(s) 72, 246, 594, 1000, 1260
PspPI GGNCC 5 cut(s) 71, 84, 114, 192, 245
PstI CTGCAG 1 cut(s) 496
PsuI RGATCY 1 cut(s) 1497
PvuI CGATCG 1 cut(s) 867
PvuII CAGCTG 1 cut(s) 883
RsaI GTAC 2 cut(s) 154, 1226
RsaNI GTAC 2 cut(s) 153, 1225
RseI CAYNNNNRTG 1 cut(s) 1487
Rsr2I CGGWCCG 1 cut(s) 84
RsrII CGGWCCG 1 cut(s) 84
SaqAI TTAA 3 cut(s) 294, 830, 1479
SatI GCNGC 6 cut(s) 993, 1101, 1170, 1201, 1297, 1305
Sau3AI GATC 8 cut(s) 54, 309, 354, 541, 612, 864, 1491, 1497
Sau96I GGNCC 5 cut(s) 71, 84, 114, 192, 245
SchI GAGTC 5 cut(s) 169, 1049, 1271, 1394, 1448
ScrFI CCNGG 4 cut(s) 118, 528, 1334, 1449
SduI GDGCHC 1 cut(s) 65
SfaNI GCATC 6 cut(s) 84, 222, 748, 963, 1170, 1291
SfcI CTRYAG 1 cut(s) 492
SfuI TTCGAA 1 cut(s) 256
SinI GGWCC 3 cut(s) 84, 192, 245
SmiMI CAYNNNNRTG 1 cut(s) 1487
SmlI CTYRAG 2 cut(s) 650, 1357
SmoI CTYRAG 2 cut(s) 650, 1357
SphI GCATGC 1 cut(s) 680
SsiI CCGC 7 cut(s) 82, 195, 395, 981, 995, 1169, 1474
SspMI CTAG 3 cut(s) 854, 1121, 1314
StyD4I CCNGG 4 cut(s) 116, 526, 1332, 1447
StyI CCWWGG 3 cut(s) 702, 894, 1026
TaaI ACNGT 2 cut(s) 968, 1507
TaiI ACGT 2 cut(s) 68, 129
TaqI TCGA 2 cut(s) 256, 1284
TatI WGTACW 1 cut(s) 1224
TauI GCSGC 1 cut(s) 1172
TfiI GAWTC 3 cut(s) 712, 796, 890
Tru1I TTAA 3 cut(s) 294, 830, 1479
Tru9I TTAA 3 cut(s) 294, 830, 1479
TscAI CASTG 3 cut(s) 328, 1179, 1489
TseFI GTSAC 2 cut(s) 779, 868
TseI GCWGC 5 cut(s) 992, 1100, 1200, 1296, 1304
Tsp45I GTSAC 2 cut(s) 779, 868
TspDTI ATGAA 3 cut(s) 137, 215, 753
TspGWI ACGGA 3 cut(s) 76, 972, 1447
TspRI CASTG 3 cut(s) 328, 1179, 1489
VneI GTGCAC 1 cut(s) 61
VpaK11BI GGWCC 3 cut(s) 84, 192, 245
VspI ATTAAT 1 cut(s) 830
XagI CCTNNNNNAGG 1 cut(s) 650
XapI RAATTY 3 cut(s) 252, 741, 751
XceI RCATGY 2 cut(s) 680, 1424
XmnI GAANNNNTTC 1 cut(s) 812
XspI CTAG 3 cut(s) 854, 1121, 1314
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.