AT3G25960

Belongs to the pyruvate kinase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
3
OG0016515
Physical Location & Seq
Forward (+)
9498439 .. 9499932
1494 bp
Loading structure...
UTR
Exon/CDS
Intron
AT3G25960.1

Sequence Viewer

Length: 1494 bp
ATGGAGATGTTACTTGGTGGACAAGCAACCAATGGAGCTCTTCGTTCAAAGACGAAGATAGTTTGTACTCTTGGACCAGCTTCAAGATCGGTGGAGATGATTGAGAAGCTTCTCAAGGCTGGTATGAACGTAGCCCGGTTCAATTTCTCCCATGGTTCTCACTCTTACCATCAAGAAACTCTCGATAATCTCAGAACCGCCATGGACAACACTGGTATCCTCTGCGCCGTCATGCTCGACACAAAGAGTCCTGTGATACGAACCGGGTTTCTCAAAGAAGGCAAACCGATACAGCTAAAGCAAGGTCAAGAGATCACCATCTCAATTGATTACAAGATACAAGGAGATTCCAACACTATCTCCATGAGCTACAAGAAACTTGCAGAGGATCTCAAACCTGGTGACGTGATCCTTTGTTCAGACGGAACAATCTCTCTAAATGTCTTGTCCTGTGACAAGTATCTCGGTCTTGTTCGTTGCCGATGCGAGAACTCTGCTCTTCTTGGAGAAAGAAAAAACGTTAATCTTCCTGGAATTGTAGTTGATCTCCCAACACTCACTGAGAAAGATAAAGAAGATATTATGCAATGGGGAGTTCCGAACAAAATCGACATTATCGCTCTCTCTTTTGTTCGTAAAGGATCTGACCTAATCCAAGTAAGGAAATTACTTGGAGAGCACTCAAAGAGCATCATGCTTATGTCAAAGGTGGAGAATCAAGAAGGAGTAATGAATTTCGATAAGATCCTGGAGAATTCTGATGCTTTCATGGTGGCTAGAGGAGATCTTGGGATGGAGATACCAATCGAAAAGATGTTTCTTGCTCAGAAAACAATGATCAACAAGGCTAATGCTCATGGGAAACCAGTGGTCACAGCCACACAGATGCTTGAATCCATGACAGTCTCTCCTCGCCCGACTAGAGCTGAAGCTACCGACGTAGCAAACGCGGTTCTTGACGGGACAGATTGCGTTATGCTTAGCGGAGAAACTGCTGCTGGAGCTCACCCTGAGACTGCCGTGTTAACAATGTCAAGGATCTGCAAAGAAGCAGAGGATTTCATAGATTACGACATTCTCCACAAGAAAACCCTAGGAATGGTTTCGTTACCGTTATCTCCAATCGAGAGCTTAGCTGCTTCAGCTGTTTCGACTGCGCGGAGTGTTTTCGCGTCCGCGATCGTTGTTCTCACTAGAGGCGGTTACACGGCGGAGCTTGTGGCTAAATACAGGCCAAGCGTTCCGATTCTGTCGGTGATTATGCCAGAGATTGCTGAATGCTCGGACTCGGTAGCTCATGTGGCGAGACGTGGTTTGATTTACCGTGGGATTATTCCGGTGGTGGGATGTTCAGCTAGAGATTCAACGGAGGAGATGATTAGATTAGCTATAGGATTTGCCAAGACGAAGGGAATTTGTAAGACTGGAGATTCCATTGTGGCTTTGCACAAGATTGATGGTTCATCTATTGTCAGGATAGTGAGCGTCGAGTAA

Protein Analysis

497

Amino Acids

53.78

Weight (kDa)

7.09

Isoelectric Point (pI)

32.17

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PK PF00224 17 - 341 3.4e-142 Pyruvate kinase, barrel domain
PK_C PF02887 376 - 485 6.7e-23 Pyruvate kinase, alpha/beta domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016515)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G04050 AT3G25960 AT3G55650
fragaria_vesca FvH4_3g14970
malus_domestica MD10G1210700.v1.1
prunus_persica Prupe.4G134000_v2.0.a1
rosa_chinensis RchiOBHm_Chr5g0024561
rosa_laevigata RLG00000032807
rosa_multiflora Rmu_sc0005699.1_g000007
rosa_rugosa Rorug05G0084000
rosa_samantha Rh5AG175700 Rh5CG189100 Rh5DG174800
rosa_wichuraiana Rw5G015880

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 4 cut(s) 950, 1159, 1172, 1178
AciI CCGC 7 cut(s) 198, 950, 984, 1159, 1176, 1200, 1211
AclI AACGTT 1 cut(s) 519
AclWI GGATC 5 cut(s) 396, 403, 649, 739, 1046
AcsI RAATTY 3 cut(s) 733, 754, 1413
AcuI CTGAAG 2 cut(s) 948, 1125
AfaI GTAC 1 cut(s) 67
AfiI CCNNNNNNNGG 2 cut(s) 1099, 1343
AgsI TTSAA 5 cut(s) 48, 84, 142, 893, 1365
AjiI CACGTC 2 cut(s) 406, 1310
AjnI CCWGG 3 cut(s) 397, 529, 747
Alw21I GWGCWC 3 cut(s) 40, 681, 1006
Alw26I GTCTC 3 cut(s) 910, 1007, 1300
AlwI GGATC 5 cut(s) 396, 403, 649, 739, 1046
AoxI GGCC 1 cut(s) 1232
ApeKI GCWGC 2 cut(s) 995, 1136
ApoI RAATTY 3 cut(s) 733, 754, 1413
Asp700I GAANNNNTTC 1 cut(s) 1102
AspA2I CCTAGG 1 cut(s) 1093
AspLEI GCGC 2 cut(s) 227, 1159
AspS9I GGNCC 1 cut(s) 74
AsuC2I CCSGG 2 cut(s) 136, 265
AsuHPI GGTGA 4 cut(s) 307, 413, 998, 1267
AvaII GGWCC 1 cut(s) 74
AvrII CCTAGG 1 cut(s) 1093
BaeI ACNNNNGTAYC 2 cut(s) 199, 232
BanII GRGCYC 2 cut(s) 40, 1006
Bbv12I GWGCWC 3 cut(s) 40, 681, 1006
BbvI GCAGC 2 cut(s) 982, 1123
BccI CCATC 4 cut(s) 177, 326, 787, 1451
BceAI ACGGC 3 cut(s) 212, 1004, 1224
BcgI CGANNNNNNTGC 2 cut(s) 476, 510
BciT130I CCWGG 3 cut(s) 399, 531, 749
BciVI GTATCC 1 cut(s) 227
BclI TGATCA 1 cut(s) 837
BcnI CCSGG 2 cut(s) 136, 265
BcoDI GTCTC 3 cut(s) 910, 1007, 1300
BfaI CTAG 5 cut(s) 777, 921, 1094, 1194, 1356
BfmI CTRYAG 1 cut(s) 1389
BfuI GTATCC 1 cut(s) 227
BglII AGATCT 1 cut(s) 784
BisI GCNGC 2 cut(s) 996, 1137
BlnI CCTAGG 1 cut(s) 1093
BlpI GCTNAGC 2 cut(s) 980, 1132
BlsI GCNGC 2 cut(s) 997, 1138
Bme1390I CCNGG 5 cut(s) 136, 265, 399, 531, 749
Bme18I GGWCC 1 cut(s) 74
BmgBI CACGTC 2 cut(s) 406, 1310
BmgT120I GGNCC 1 cut(s) 74
BmrFI CCNGG 5 cut(s) 136, 265, 399, 531, 749
BmsI GCATC 4 cut(s) 473, 699, 751, 876
BpmI CTGGAG 3 cut(s) 770, 1020, 1446
Bpu1102I GCTNAGC 2 cut(s) 980, 1132
BpuEI CTTGAG 1 cut(s) 98
BpuMI CCSGG 2 cut(s) 136, 265
BsaBI GATNNNNATC 2 cut(s) 317, 803
BsaJI CCNNGG 4 cut(s) 151, 201, 1093, 1324
BsaWI WCCGGW 1 cut(s) 1336
BsaXI ACNNNNNCTCC 4 cut(s) 344, 374, 892, 922
Bsc4I CCNNNNNNNGG 2 cut(s) 1099, 1343
Bse1I ACTGG 3 cut(s) 217, 866, 1429
Bse3DI GCAATG 1 cut(s) 593
Bse8I GATNNNNATC 2 cut(s) 317, 803
BseBI CCWGG 3 cut(s) 399, 531, 749
BseDI CCNNGG 4 cut(s) 151, 201, 1093, 1324
BseGI GGATG 2 cut(s) 798, 1352
BseJI GATNNNNATC 2 cut(s) 317, 803
BseLI CCNNNNNNNGG 2 cut(s) 1099, 1343
BseMI GCAATG 1 cut(s) 593
BseMII CTCAG 4 cut(s) 205, 552, 839, 1002
BseNI ACTGG 3 cut(s) 217, 866, 1429
BseRI GAGGAG 3 cut(s) 795, 900, 1385
BseXI GCAGC 2 cut(s) 982, 1123
Bsh1236I CGCG 4 cut(s) 950, 1159, 1172, 1178
Bsh1285I CGRYCG 1 cut(s) 1182
BshFI GGCC 1 cut(s) 1234
BsiEI CGRYCG 1 cut(s) 1182
BsiHKAI GWGCWC 3 cut(s) 40, 681, 1006
BsiSI CCGG 3 cut(s) 136, 264, 1337
BslFI GGGAC 1 cut(s) 976
BslI CCNNNNNNNGG 2 cut(s) 1099, 1343
BsmAI GTCTC 3 cut(s) 910, 1007, 1300
BsmBI CGTCTC 1 cut(s) 1300
BsmFI GGGAC 1 cut(s) 976
BsmI GAATGC 1 cut(s) 1283
BsnI GGCC 1 cut(s) 1234
Bsp1286I GDGCHC 3 cut(s) 40, 681, 1006
Bsp1720I GCTNAGC 2 cut(s) 980, 1132
Bsp19I CCATGG 2 cut(s) 151, 201
BspACI CCGC 7 cut(s) 198, 950, 984, 1159, 1176, 1200, 1211
BspANI GGCC 1 cut(s) 1234
BspCNI CTCAG 4 cut(s) 204, 553, 838, 1003
BspFNI CGCG 4 cut(s) 950, 1159, 1172, 1178
BspPI GGATC 5 cut(s) 396, 403, 649, 739, 1046
BspQI GCTCTTC 2 cut(s) 45, 504
BsrDI GCAATG 1 cut(s) 593
BsrI ACTGG 3 cut(s) 217, 866, 1429
BssECI CCNNGG 4 cut(s) 151, 201, 1093, 1324
BssT1I CCWWGG 3 cut(s) 151, 201, 1093
Bst2UI CCWGG 3 cut(s) 399, 531, 749
Bst4CI ACNGT 3 cut(s) 904, 1113, 1325
Bst6I CTCTTC 2 cut(s) 45, 504
BstDEI CTNAG 6 cut(s) 191, 561, 825, 980, 1011, 1132
BstDSI CCRYGG 3 cut(s) 151, 201, 1324
BstF5I GGATG 2 cut(s) 798, 1352
BstFNI CGCG 4 cut(s) 950, 1159, 1172, 1178
BstHHI GCGC 2 cut(s) 227, 1159
BstMAI GTCTC 3 cut(s) 910, 1007, 1300
BstMCI CGRYCG 1 cut(s) 1182
BstMWI GCNNNNNNNGC 3 cut(s) 1001, 1142, 1301
BstNI CCWGG 3 cut(s) 399, 531, 749
BstSCI CCNGG 5 cut(s) 134, 263, 397, 529, 747
BstSFI CTRYAG 1 cut(s) 1389
BstUI CGCG 4 cut(s) 950, 1159, 1172, 1178
BstV1I GCAGC 2 cut(s) 982, 1123
BstX2I RGATCY 5 cut(s) 388, 641, 744, 784, 1038
BstYI RGATCY 5 cut(s) 388, 641, 744, 784, 1038
BsuI GTATCC 1 cut(s) 227
BsuRI GGCC 1 cut(s) 1234
BtgI CCRYGG 3 cut(s) 151, 201, 1324
BtrI CACGTC 2 cut(s) 406, 1310
BtsCI GGATG 2 cut(s) 798, 1352
BtsIMutI CAGTG 3 cut(s) 210, 558, 873
CfoI GCGC 2 cut(s) 227, 1159
Cfr13I GGNCC 1 cut(s) 74
CseI GACGC 2 cut(s) 1161, 1474
CsiI ACCWGGT 1 cut(s) 397
Csp6I GTAC 1 cut(s) 66
CspCI CAANNNNNGTGG 2 cut(s) 72, 107
CviAII CATG 9 cut(s) 152, 202, 232, 364, 694, 769, 857, 898, 1298
CviQI GTAC 1 cut(s) 66
DdeI CTNAG 6 cut(s) 191, 561, 825, 980, 1011, 1132
Eam1104I CTCTTC 2 cut(s) 45, 504
EarI CTCTTC 2 cut(s) 45, 504
EciI GGCGGA 1 cut(s) 1226
Ecl136II GAGCTC 2 cut(s) 38, 1004
Eco130I CCWWGG 3 cut(s) 151, 201, 1093
Eco24I GRGCYC 2 cut(s) 40, 1006
Eco47I GGWCC 1 cut(s) 74
Eco53kI GAGCTC 2 cut(s) 38, 1004
Eco57I CTGAAG 2 cut(s) 948, 1125
EcoICRI GAGCTC 2 cut(s) 38, 1004
EcoRI GAATTC 1 cut(s) 754
EcoRII CCWGG 3 cut(s) 397, 529, 747
EcoT14I CCWWGG 3 cut(s) 151, 201, 1093
EcoT38I GRGCYC 2 cut(s) 40, 1006
ErhI CCWWGG 3 cut(s) 151, 201, 1093
Esp3I CGTCTC 1 cut(s) 1300
FaeI CATG 9 cut(s) 155, 205, 235, 367, 697, 772, 860, 901, 1301
FaqI GGGAC 1 cut(s) 976
FatI CATG 9 cut(s) 151, 201, 231, 363, 693, 768, 856, 897, 1297
FbaI TGATCA 1 cut(s) 837
Fnu4HI GCNGC 2 cut(s) 996, 1137
FokI GGATG 2 cut(s) 805, 1359
FriOI GRGCYC 2 cut(s) 40, 1006
Fsp4HI GCNGC 2 cut(s) 996, 1137
FspBI CTAG 5 cut(s) 777, 921, 1094, 1194, 1356
GlaI GCGC 2 cut(s) 226, 1158
GluI GCNGC 2 cut(s) 996, 1137
GsuI CTGGAG 3 cut(s) 770, 1020, 1446
HaeIII GGCC 1 cut(s) 1234
HapII CCGG 3 cut(s) 136, 264, 1337
HgaI GACGC 2 cut(s) 1161, 1474
HhaI GCGC 2 cut(s) 227, 1159
Hin1II CATG 9 cut(s) 155, 205, 235, 367, 697, 772, 860, 901, 1301
Hin6I GCGC 2 cut(s) 225, 1157
HinP1I GCGC 2 cut(s) 225, 1157
HincII GTYRAC 1 cut(s) 1026
HindII GTYRAC 1 cut(s) 1026
HindIII AAGCTT 1 cut(s) 107
HinfI GANTC 8 cut(s) 247, 347, 715, 893, 1246, 1286, 1361, 1430
HpaI GTTAAC 1 cut(s) 1026
HpaII CCGG 3 cut(s) 136, 264, 1337
HphI GGTGA 4 cut(s) 307, 413, 998, 1267
Hpy166II GTNNAC 2 cut(s) 20, 1026
Hpy188I TCNGA 8 cut(s) 194, 421, 600, 646, 760, 828, 1245, 1285
Hpy188III TCNNGA 8 cut(s) 84, 173, 182, 308, 719, 956, 1126, 1474
Hpy8I GTNNAC 2 cut(s) 20, 1026
Hpy99I CGWCG 2 cut(s) 941, 1490
HpyAV CCTTC 3 cut(s) 272, 716, 1402
HpyCH4III ACNGT 3 cut(s) 904, 1113, 1325
HpyCH4IV ACGT 5 cut(s) 129, 405, 519, 939, 1309
HpyCH4V TGCA 4 cut(s) 383, 586, 1044, 1447
HpyF10VI GCNNNNNNNGC 3 cut(s) 1001, 1142, 1301
HpyF3I CTNAG 6 cut(s) 191, 561, 825, 980, 1011, 1132
HpySE526I ACGT 5 cut(s) 129, 405, 519, 939, 1309
Hsp92II CATG 9 cut(s) 155, 205, 235, 367, 697, 772, 860, 901, 1301
HspAI GCGC 2 cut(s) 225, 1157
Ksp22I TGATCA 1 cut(s) 837
KspAI GTTAAC 1 cut(s) 1026
LguI GCTCTTC 2 cut(s) 45, 504
LmnI GCTCC 3 cut(s) 35, 1001, 1213
Lsp1109I GCAGC 2 cut(s) 982, 1123
LweI GCATC 4 cut(s) 473, 699, 751, 876
MabI ACCWGGT 1 cut(s) 397
MaeI CTAG 5 cut(s) 777, 921, 1094, 1194, 1356
MaeII ACGT 5 cut(s) 129, 405, 519, 939, 1309
MaeIII GTNAC 6 cut(s) 9, 401, 452, 871, 1107, 1202
MboII GAAGA 5 cut(s) 32, 67, 491, 518, 587
MfeI CAATTG 1 cut(s) 324
MflI RGATCY 5 cut(s) 388, 641, 744, 784, 1038
MhlI GDGCHC 3 cut(s) 40, 681, 1006
MluCI AATT 7 cut(s) 142, 324, 534, 665, 733, 754, 1413
MlyI GAGTC 2 cut(s) 256, 1280
MmeI TCCRAC 1 cut(s) 375
MnlI CCTC 7 cut(s) 230, 379, 773, 921, 1048, 1190, 1363
MroXI GAANNNNTTC 1 cut(s) 1102
MseI TTAA 2 cut(s) 522, 1025
MslI CAYNNNNRTG 2 cut(s) 698, 884
MspA1I CMGCKG 1 cut(s) 1145
MspI CCGG 3 cut(s) 136, 264, 1337
MspR9I CCNGG 5 cut(s) 136, 265, 399, 531, 749
MunI CAATTG 1 cut(s) 324
Mva1269I GAATGC 1 cut(s) 1283
MvaI CCWGG 3 cut(s) 399, 531, 749
MvnI CGCG 4 cut(s) 950, 1159, 1172, 1178
MwoI GCNNNNNNNGC 3 cut(s) 1001, 1142, 1301
NciI CCSGG 2 cut(s) 136, 265
NcoI CCATGG 2 cut(s) 151, 201
NlaIII CATG 9 cut(s) 155, 205, 235, 367, 697, 772, 860, 901, 1301
NmuCI GTSAC 3 cut(s) 401, 452, 871
PciSI GCTCTTC 2 cut(s) 45, 504
PctI GAATGC 1 cut(s) 1283
PdmI GAANNNNTTC 1 cut(s) 1102
PfeI GAWTC 6 cut(s) 347, 715, 893, 1246, 1361, 1430
PfoI TCCNGGA 2 cut(s) 529, 747
PkrI GCNGC 2 cut(s) 997, 1138
Ple19I CGATCG 1 cut(s) 1182
PleI GAGTC 2 cut(s) 255, 1280
PpsI GAGTC 2 cut(s) 255, 1280
Psp124BI GAGCTC 2 cut(s) 40, 1006
Psp1406I AACGTT 1 cut(s) 519
Psp6I CCWGG 3 cut(s) 397, 529, 747
PspGI CCWGG 3 cut(s) 397, 529, 747
PspPI GGNCC 1 cut(s) 74
PsuI RGATCY 5 cut(s) 388, 641, 744, 784, 1038
PvuI CGATCG 1 cut(s) 1182
PvuII CAGCTG 1 cut(s) 1145
RsaI GTAC 1 cut(s) 67
RsaNI GTAC 1 cut(s) 66
RseI CAYNNNNRTG 2 cut(s) 698, 884
SacI GAGCTC 2 cut(s) 40, 1006
SapI GCTCTTC 2 cut(s) 45, 504
SaqAI TTAA 2 cut(s) 522, 1025
SatI GCNGC 2 cut(s) 996, 1137
Sau96I GGNCC 1 cut(s) 74
SchI GAGTC 2 cut(s) 256, 1280
ScrFI CCNGG 5 cut(s) 136, 265, 399, 531, 749
SduI GDGCHC 3 cut(s) 40, 681, 1006
SexAI ACCWGGT 1 cut(s) 397
SfaNI GCATC 4 cut(s) 473, 699, 751, 876
SfcI CTRYAG 1 cut(s) 1389
SinI GGWCC 1 cut(s) 74
SmiMI CAYNNNNRTG 2 cut(s) 698, 884
SmlI CTYRAG 1 cut(s) 113
SmoI CTYRAG 1 cut(s) 113
Sse9I AATT 7 cut(s) 142, 324, 534, 665, 733, 754, 1413
SsiI CCGC 7 cut(s) 198, 950, 984, 1159, 1176, 1200, 1211
SspMI CTAG 5 cut(s) 777, 921, 1094, 1194, 1356
SstI GAGCTC 2 cut(s) 40, 1006
StyD4I CCNGG 5 cut(s) 134, 263, 397, 529, 747
StyI CCWWGG 3 cut(s) 151, 201, 1093
TaaI ACNGT 3 cut(s) 904, 1113, 1325
TaiI ACGT 5 cut(s) 132, 408, 522, 942, 1312
TaqI TCGA 8 cut(s) 183, 237, 609, 738, 807, 1125, 1151, 1488
TaqII GACCGA 1 cut(s) 455
TasI AATT 7 cut(s) 142, 324, 534, 665, 733, 754, 1413
TatI WGTACW 1 cut(s) 65
TfiI GAWTC 6 cut(s) 347, 715, 893, 1246, 1361, 1430
Tru1I TTAA 2 cut(s) 522, 1025
Tru9I TTAA 2 cut(s) 522, 1025
TscAI CASTG 3 cut(s) 217, 565, 873
TseFI GTSAC 3 cut(s) 401, 452, 871
TseI GCWGC 2 cut(s) 995, 1136
Tsp45I GTSAC 3 cut(s) 401, 452, 871
TspDTI ATGAA 5 cut(s) 140, 746, 757, 1051, 1452
TspGWI ACGGA 2 cut(s) 438, 1382
TspRI CASTG 3 cut(s) 217, 565, 873
VpaK11BI GGWCC 1 cut(s) 74
XapI RAATTY 3 cut(s) 733, 754, 1413
XmaJI CCTAGG 1 cut(s) 1093
XmnI GAANNNNTTC 1 cut(s) 1102
XspI CTAG 5 cut(s) 777, 921, 1094, 1194, 1356
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.