AT3G04590

DNA-binding

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
3
OG0011499
Physical Location & Seq
Reverse (-)
1238853 .. 1241804
2952 bp
Loading structure...
UTR
Exon/CDS
Intron
AT3G04590.1

Sequence Viewer

Length: 930 bp
ATGGATCCTAACGAAAGCCACCATCACCACCAACAACAACAGCTCCATCACCTCCACCAACAGCAACAGCAACAGCAGCAGCAGCAACGACTCACTTCTCCTTACTTCCACCACCAACTACAGCACCATCACCACCTTCCAACCACCGTAGCAACCACCGCTTCTACCGGAAACGCCGTTCCATCTTCCAACAATGGGCTTTTCCCTCCGCAGCCTCAGCCACAGCACCAGCCTAATGATGGGTCATCTTCTCTCGCGGTGTACCCTCATTCAGTTCCGTCCTCGGCTGTGACGGCGCCGATGGAGCCGGTAAAGAGGAAGAGGGGTCGACCAAGAAAGTATGTGACGCCGGAACAAGCCCTAGCGGCTAAGAAATTGGCGTCTTCTGCGAGTAGTTCGTCTGCTAAACAGAGGCGAGAGCTTGCTGCTGTTACCGGTGGTACGGTATCGACTAATTCCGGGTCATCCAAGAAATCTCAGCTTGGTTCTGTCGGGAAAACTGGACAATGTTTTACTCCGCATATTGTTAATATAGCTCCTGGCGAGGATGTGGTCCAGAAAATTATGATGTTCGCAAACCAAAGCAAGCATGAACTATGCGTTCTTTCTGCATCAGGCACTATCTCTAATGCATCCTTGCGCCAACCGGCTCCATCAGGAGGCAACTTACCATATGAGGGTCAATACGAGATTCTCTCACTATCTGGATCCTATATCCGAACTGAACAAGGTGGTAAATCCGGCGGCCTTAGCGTTTCTTTATCTGCTTCAGATGGTCAGATCATCGGTGGAGCGATTGGTAGCCATCTCACAGCTGCTGGCCCGGTTCAGGTGATTCTTGGTACGTTTCAGCTTGATAGAAAGAAGGATGCCGCCGGGAGTGGTGGGAAAGGGGATGCTTCAAACAGTGGAAAAGTGTGTAGAGTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF296

Protein Analysis

309

Amino Acids

32.58

Weight (kDa)

9.71

Isoelectric Point (pI)

49.69

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PCC PF03479 172 - 284 2.8e-23 Plants and Prokaryotes Conserved (PCC) domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011499)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G04590 AT3G04590
fragaria_vesca FvH4_6g02360 FvH4_6g02360
malus_domestica MD00G1114400.v1.1 MD12G1245000.v1.1
prunus_persica Prupe.6G347600_v2.0.a1 Prupe.6G347600_v2.0.a1
pyrus_communis pycom04g20220 pycom12g22470
rosa_chinensis RchiOBHm_Chr3g0450121
rosa_laevigata RLG00000025754
rosa_multiflora Rmu_ssc0000116.1_g000066
rosa_roxburghii Rroxscaffold_6G00427890
rosa_rugosa Rorug02G0625700
rosa_samantha Rh3AG024900 Rh3BG025500 Rh3CG024400 Rh3DG025500
rosa_wichuraiana Rw3G001990

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 295
AccI GTMKAC 1 cut(s) 328
AccII CGCG 1 cut(s) 257
AciI CCGC 7 cut(s) 159, 209, 257, 365, 518, 744, 873
AclWI GGATC 3 cut(s) 12, 702, 715
AcuI CTGAAG 1 cut(s) 753
AcyI GRCGYC 3 cut(s) 296, 347, 380
AfaI GTAC 3 cut(s) 263, 442, 844
AfiI CCNNNNNNNGG 5 cut(s) 195, 239, 659, 677, 829
AgeI ACCGGT 1 cut(s) 434
AgsI TTSAA 1 cut(s) 903
AjnI CCWGG 1 cut(s) 538
AloI GAACNNNNNNTCC 2 cut(s) 162, 194
AluBI AGCT 6 cut(s) 43, 421, 481, 536, 815, 853
AluI AGCT 6 cut(s) 43, 421, 481, 536, 815, 853
AlwI GGATC 3 cut(s) 12, 702, 715
AlwNI CAGNNNCTG 1 cut(s) 818
AoxI GGCC 2 cut(s) 745, 820
ApeKI GCWGC 6 cut(s) 76, 79, 82, 211, 425, 815
AsiGI ACCGGT 1 cut(s) 434
AspLEI GCGC 2 cut(s) 298, 642
AspS9I GGNCC 2 cut(s) 553, 821
AsuC2I CCSGG 3 cut(s) 460, 824, 877
AsuHPI GGTGA 4 cut(s) 17, 41, 122, 844
AvaII GGWCC 1 cut(s) 553
BamHI GGATCC 2 cut(s) 4, 707
BanI GGYRCC 1 cut(s) 295
BbsI GAAGAC 1 cut(s) 375
BbvCI CCTCAGC 1 cut(s) 216
BbvI GCAGC 6 cut(s) 88, 91, 94, 223, 412, 802
BccI CCATC 9 cut(s) 30, 54, 135, 190, 233, 295, 661, 767, 813
BceAI ACGGC 2 cut(s) 161, 309
BciT130I CCWGG 1 cut(s) 540
BcnI CCSGG 3 cut(s) 460, 824, 877
BfaI CTAG 1 cut(s) 362
BfmI CTRYAG 1 cut(s) 119
BfoI RGCGCY 1 cut(s) 299
BglI GCCNNNNNGGC 1 cut(s) 365
BisI GCNGC 9 cut(s) 77, 80, 83, 212, 366, 426, 745, 816, 873
BlsI GCNGC 9 cut(s) 78, 81, 84, 213, 367, 427, 746, 817, 874
Bme1390I CCNGG 4 cut(s) 460, 540, 824, 877
Bme18I GGWCC 1 cut(s) 553
BmgT120I GGNCC 2 cut(s) 553, 821
BmiI GGNNCC 5 cut(s) 6, 297, 306, 651, 709
BmrFI CCNGG 4 cut(s) 460, 540, 824, 877
BmsI GCATC 4 cut(s) 620, 641, 859, 886
BpiI GAAGAC 1 cut(s) 375
BplI GAGNNNNNCTC 2 cut(s) 680, 712
Bpu10I CCTNAGC 2 cut(s) 216, 749
BpuMI CCSGG 3 cut(s) 460, 824, 877
BsaHI GRCGYC 3 cut(s) 296, 347, 380
BsaJI CCNNGG 1 cut(s) 282
BsaWI WCCGGW 2 cut(s) 167, 434
BsaXI ACNNNNNCTCC 2 cut(s) 27, 57
Bsc4I CCNNNNNNNGG 5 cut(s) 195, 239, 659, 677, 829
Bse118I RCCGGY 3 cut(s) 307, 434, 646
Bse1I ACTGG 1 cut(s) 505
BseBI CCWGG 1 cut(s) 540
BseDI CCNNGG 1 cut(s) 282
BseGI GGATG 5 cut(s) 464, 553, 632, 874, 901
BseLI CCNNNNNNNGG 5 cut(s) 195, 239, 659, 677, 829
BseMII CTCAG 2 cut(s) 230, 491
BseNI ACTGG 1 cut(s) 505
BseXI GCAGC 6 cut(s) 88, 91, 94, 223, 412, 802
Bsh1236I CGCG 1 cut(s) 257
BshFI GGCC 2 cut(s) 747, 822
BshNI GGYRCC 1 cut(s) 295
BshTI ACCGGT 1 cut(s) 434
BsiSI CCGG 9 cut(s) 168, 308, 350, 435, 459, 647, 741, 824, 876
BslI CCNNNNNNNGG 5 cut(s) 195, 239, 659, 677, 829
BsnI GGCC 2 cut(s) 747, 822
Bsp143I GATC 3 cut(s) 4, 707, 780
BspACI CCGC 7 cut(s) 159, 209, 257, 365, 518, 744, 873
BspANI GGCC 2 cut(s) 747, 822
BspCNI CTCAG 2 cut(s) 229, 490
BspFNI CGCG 1 cut(s) 257
BspLI GGNNCC 5 cut(s) 6, 297, 306, 651, 709
BspPI GGATC 3 cut(s) 12, 702, 715
BspT107I GGYRCC 1 cut(s) 295
BsrFI RCCGGY 3 cut(s) 307, 434, 646
BsrI ACTGG 1 cut(s) 505
BssAI RCCGGY 3 cut(s) 307, 434, 646
BssECI CCNNGG 1 cut(s) 282
BssMI GATC 3 cut(s) 4, 707, 780
BssNI GRCGYC 3 cut(s) 296, 347, 380
Bst2UI CCWGG 1 cut(s) 540
Bst4CI ACNGT 3 cut(s) 148, 445, 908
Bst6I CTCTTC 1 cut(s) 314
BstACI GRCGYC 3 cut(s) 296, 347, 380
BstC8I GCNNGC 3 cut(s) 423, 587, 820
BstDEI CTNAG 4 cut(s) 216, 369, 477, 749
BstF5I GGATG 5 cut(s) 464, 553, 632, 874, 901
BstFNI CGCG 1 cut(s) 257
BstH2I RGCGCY 1 cut(s) 299
BstHHI GCGC 2 cut(s) 298, 642
BstKTI GATC 3 cut(s) 7, 710, 783
BstMBI GATC 3 cut(s) 4, 707, 780
BstMWI GCNNNNNNNGC 9 cut(s) 76, 82, 158, 217, 293, 304, 365, 386, 750
BstNI CCWGG 1 cut(s) 540
BstSCI CCNGG 4 cut(s) 458, 538, 822, 875
BstSFI CTRYAG 1 cut(s) 119
BstUI CGCG 1 cut(s) 257
BstV1I GCAGC 6 cut(s) 88, 91, 94, 223, 412, 802
BstV2I GAAGAC 1 cut(s) 375
BstX2I RGATCY 2 cut(s) 4, 707
BstYI RGATCY 2 cut(s) 4, 707
BsuRI GGCC 2 cut(s) 747, 822
BtsCI GGATG 5 cut(s) 464, 553, 632, 874, 901
BtsIMutI CAGTG 1 cut(s) 913
Cac8I GCNNGC 3 cut(s) 423, 587, 820
CaiI CAGNNNCTG 1 cut(s) 818
CfoI GCGC 2 cut(s) 298, 642
Cfr10I RCCGGY 3 cut(s) 307, 434, 646
Cfr13I GGNCC 2 cut(s) 553, 821
CseI GACGC 2 cut(s) 355, 369
Csp6I GTAC 3 cut(s) 262, 441, 843
CspAI ACCGGT 1 cut(s) 434
CviAII CATG 1 cut(s) 590
CviQI GTAC 3 cut(s) 262, 441, 843
DdeI CTNAG 4 cut(s) 216, 369, 477, 749
DinI GGCGCC 1 cut(s) 297
DpnI GATC 3 cut(s) 6, 709, 782
DpnII GATC 3 cut(s) 4, 707, 780
Eam1104I CTCTTC 1 cut(s) 314
EarI CTCTTC 1 cut(s) 314
Eco47I GGWCC 1 cut(s) 553
Eco57I CTGAAG 1 cut(s) 753
EcoRII CCWGG 1 cut(s) 538
EcoT22I ATGCAT 1 cut(s) 634
EgeI GGCGCC 1 cut(s) 297
EheI GGCGCC 1 cut(s) 297
FaeI CATG 1 cut(s) 593
FaiI YATR 9 cut(s) 342, 522, 533, 566, 591, 598, 673, 675, 714
FatI CATG 1 cut(s) 589
FauNDI CATATG 1 cut(s) 673
FblI GTMKAC 1 cut(s) 328
Fnu4HI GCNGC 9 cut(s) 77, 80, 83, 212, 366, 426, 745, 816, 873
FokI GGATG 5 cut(s) 451, 560, 619, 881, 908
Fsp4HI GCNGC 9 cut(s) 77, 80, 83, 212, 366, 426, 745, 816, 873
FspBI CTAG 1 cut(s) 362
GlaI GCGC 2 cut(s) 297, 641
GluI GCNGC 9 cut(s) 77, 80, 83, 212, 366, 426, 745, 816, 873
HaeII RGCGCY 1 cut(s) 299
HaeIII GGCC 2 cut(s) 747, 822
HapII CCGG 9 cut(s) 168, 308, 350, 435, 459, 647, 741, 824, 876
HgaI GACGC 2 cut(s) 355, 369
HhaI GCGC 2 cut(s) 298, 642
Hin1I GRCGYC 3 cut(s) 296, 347, 380
Hin1II CATG 1 cut(s) 593
Hin6I GCGC 2 cut(s) 296, 640
HinP1I GCGC 2 cut(s) 296, 640
HincII GTYRAC 1 cut(s) 329
HindII GTYRAC 1 cut(s) 329
HinfI GANTC 3 cut(s) 90, 691, 835
HpaII CCGG 9 cut(s) 168, 308, 350, 435, 459, 647, 741, 824, 876
HphI GGTGA 4 cut(s) 17, 41, 122, 844
Hpy166II GTNNAC 2 cut(s) 262, 329
Hpy188I TCNGA 3 cut(s) 719, 772, 780
Hpy188III TCNNGA 4 cut(s) 493, 556, 657, 705
Hpy8I GTNNAC 2 cut(s) 262, 329
HpyAV CCTTC 2 cut(s) 146, 859
HpyCH4III ACNGT 3 cut(s) 148, 445, 908
HpyCH4IV ACGT 1 cut(s) 845
HpyCH4V TGCA 2 cut(s) 611, 632
HpyF10VI GCNNNNNNNGC 9 cut(s) 76, 82, 158, 217, 293, 304, 365, 386, 750
HpyF3I CTNAG 4 cut(s) 216, 369, 477, 749
HpySE526I ACGT 1 cut(s) 845
Hsp92I GRCGYC 3 cut(s) 296, 347, 380
Hsp92II CATG 1 cut(s) 593
HspAI GCGC 2 cut(s) 296, 640
KasI GGCGCC 1 cut(s) 295
Kzo9I GATC 3 cut(s) 4, 707, 780
LmnI GCTCC 5 cut(s) 48, 304, 541, 655, 791
Lsp1109I GCAGC 6 cut(s) 88, 91, 94, 223, 412, 802
LweI GCATC 4 cut(s) 620, 641, 859, 886
MaeI CTAG 1 cut(s) 362
MaeII ACGT 1 cut(s) 845
MaeIII GTNAC 3 cut(s) 289, 343, 430
MalI GATC 3 cut(s) 6, 709, 782
MboI GATC 3 cut(s) 4, 707, 780
MboII GAAGA 4 cut(s) 177, 240, 331, 375
MflI RGATCY 2 cut(s) 4, 707
MluCI AATT 3 cut(s) 374, 454, 561
Mly113I GGCGCC 1 cut(s) 296
MlyI GAGTC 1 cut(s) 84
MmeI TCCRAC 2 cut(s) 164, 213
Mph1103I ATGCAT 1 cut(s) 634
MseI TTAA 1 cut(s) 528
MspA1I CMGCKG 1 cut(s) 815
MspI CCGG 9 cut(s) 168, 308, 350, 435, 459, 647, 741, 824, 876
MspR9I CCNGG 4 cut(s) 460, 540, 824, 877
MvaI CCWGG 1 cut(s) 540
MvnI CGCG 1 cut(s) 257
MwoI GCNNNNNNNGC 9 cut(s) 76, 82, 158, 217, 293, 304, 365, 386, 750
NarI GGCGCC 1 cut(s) 296
NciI CCSGG 3 cut(s) 460, 824, 877
NdeI CATATG 1 cut(s) 673
NdeII GATC 3 cut(s) 4, 707, 780
NlaIII CATG 1 cut(s) 593
NlaIV GGNNCC 5 cut(s) 6, 297, 306, 651, 709
NmeAIII GCCGAG 1 cut(s) 263
NmuCI GTSAC 2 cut(s) 289, 343
NsiI ATGCAT 1 cut(s) 634
PfeI GAWTC 2 cut(s) 691, 835
PinAI ACCGGT 1 cut(s) 434
PkrI GCNGC 9 cut(s) 78, 81, 84, 213, 367, 427, 746, 817, 874
PleI GAGTC 1 cut(s) 84
PluTI GGCGCC 1 cut(s) 299
PpsI GAGTC 1 cut(s) 84
Psp6I CCWGG 1 cut(s) 538
PspGI CCWGG 1 cut(s) 538
PspN4I GGNNCC 5 cut(s) 6, 297, 306, 651, 709
PspPI GGNCC 2 cut(s) 553, 821
PstNI CAGNNNCTG 1 cut(s) 818
PsuI RGATCY 2 cut(s) 4, 707
PvuII CAGCTG 1 cut(s) 815
RsaI GTAC 3 cut(s) 263, 442, 844
RsaNI GTAC 3 cut(s) 262, 441, 843
SalI GTCGAC 1 cut(s) 327
SaqAI TTAA 1 cut(s) 528
SatI GCNGC 9 cut(s) 77, 80, 83, 212, 366, 426, 745, 816, 873
Sau3AI GATC 3 cut(s) 4, 707, 780
Sau96I GGNCC 2 cut(s) 553, 821
SchI GAGTC 1 cut(s) 84
ScrFI CCNGG 4 cut(s) 460, 540, 824, 877
SfaNI GCATC 4 cut(s) 620, 641, 859, 886
SfcI CTRYAG 1 cut(s) 119
SfoI GGCGCC 1 cut(s) 297
SinI GGWCC 1 cut(s) 553
Sse9I AATT 3 cut(s) 374, 454, 561
SsiI CCGC 7 cut(s) 159, 209, 257, 365, 518, 744, 873
SspDI GGCGCC 1 cut(s) 295
SspMI CTAG 1 cut(s) 362
StyD4I CCNGG 4 cut(s) 458, 538, 822, 875
TaaI ACNGT 3 cut(s) 148, 445, 908
TaiI ACGT 1 cut(s) 848
TaqI TCGA 2 cut(s) 328, 449
TasI AATT 3 cut(s) 374, 454, 561
TauI GCSGC 3 cut(s) 368, 747, 875
TfiI GAWTC 2 cut(s) 691, 835
Tru1I TTAA 1 cut(s) 528
Tru9I TTAA 1 cut(s) 528
TscAI CASTG 1 cut(s) 913
TseFI GTSAC 2 cut(s) 289, 343
TseI GCWGC 6 cut(s) 76, 79, 82, 211, 425, 815
Tsp45I GTSAC 2 cut(s) 289, 343
TspDTI ATGAA 1 cut(s) 606
TspGWI ACGGA 1 cut(s) 267
TspRI CASTG 1 cut(s) 913
VpaK11BI GGWCC 1 cut(s) 553
XcmI CCANNNNNNNNNTGG 1 cut(s) 236
XmiI GTMKAC 1 cut(s) 328
XspI CTAG 1 cut(s) 362
Zsp2I ATGCAT 1 cut(s) 634
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.