Prupe.6G347600_v2.0.a1

DNA-binding protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp06
OG0011499
Physical Location & Seq
Forward (+)
29734568 .. 29739564
4997 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.6G347600.1

Sequence Viewer

Length: 1068 bp
ATGGAACCTAATGAAAACCAACTGAGCTCCTACTTCCAACACCCCACCACCACCACCGGCACCGGCACCGCCGCAACTGTCACAGCCACAAACACTGCATCACCGACCAATGGGCTCTTGCCAAATACCCACTCCACTGATGGGTCCCACATGGTGTACTCTCACTCGGTGCCTTCCTCAGCGGTAACCTCGCCACTCGAGCCCGCGAAAAGGAAGCGAGGAAGGCCCAGAAAGTATGGCACACCGGAACAGGCTTTGGCAGCCAAGAAGGCGGCGACGACGTCATCTCACTCCTCATCCTCTAAGGAGAAAAAGGACCACCATGGCTCTGCCTCGCCTTCTTATTCTGGGTCTACCAAGAAATCTCAGCAGTTTTCTCTTGGCAATGCAGGGCAAGGTTTTACACCACATGTTTTAACTGTAGCTGCTGGGGAGGATGTGGGCCAGAAAATTATGTTTTTTATGCAACAGAGTAAGCGTGAAATATGCATTCTGTCTGCTTCTGGTACAATCTCAAATGCTTCTCTCCGCCAGCCAGCTACTTCAGGAGGCAACATTACATATGAGGGTCGTTTTGAGATTATTTCACTATCTGGGTCTTACGTACGTACTGACCTTGGAGGGAGGGCTGGTGGGCTCAGTGTGTGCCTATCTAGTACAGATGGGCAAATCATCGGCGGTGGAGTTGGTGGGCCCCTGAAGGCTGCAGGCCCTGTGCAGGTTATTGTCGGAACATTTATGGTTGATGCCAAGAAGGATGTCACTGCTGGTGTAAAGGGTGATGCTTCTGCTACCAAGTTGCCAACAGCTGGGGAAATGATGAATGTAAGCTTCCGCTCGGCAGTTGACTCTTCTGGAAGAACTCTCGTCCGGGGAAATGATGATCAGCAAGCTATTGGAGGAAGTCATTTTATGATTCAGGGCATGCATGTCGCACCTTCACGGCCCACAGATTGGAGGGGTGGTCCAGATGCTAGAGGCACTGGTGCTTATGAATTGACAGGAAGAGCAGGTCGTGCAGCACACCAATCTCCAGAAAATGGGGACTATGATCAAATTCCAGATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF296

Protein Analysis

356

Amino Acids

36.77

Weight (kDa)

9.24

Isoelectric Point (pI)

35.2

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011499)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G04590 AT3G04590
fragaria_vesca FvH4_6g02360 FvH4_6g02360
malus_domestica MD00G1114400.v1.1 MD12G1245000.v1.1
prunus_persica Prupe.6G347600_v2.0.a1 Prupe.6G347600_v2.0.a1
pyrus_communis pycom04g20220 pycom12g22470
rosa_chinensis RchiOBHm_Chr3g0450121
rosa_laevigata RLG00000025754
rosa_multiflora Rmu_ssc0000116.1_g000066
rosa_roxburghii Rroxscaffold_6G00427890
rosa_rugosa Rorug02G0625700
rosa_samantha Rh3AG024900 Rh3BG025500 Rh3CG024400 Rh3DG025500
rosa_wichuraiana Rw3G001990

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 284
Acc36I ACCTGC 2 cut(s) 709, 1001
AccB1I GGYRCC 3 cut(s) 59, 65, 169
AccB7I CCANNNNNTGG 3 cut(s) 809, 954, 1040
AccBSI CCGCTC 1 cut(s) 837
AccI GTMKAC 1 cut(s) 353
AccII CGCG 1 cut(s) 206
AciI CCGC 8 cut(s) 69, 72, 182, 204, 272, 529, 678, 835
AcsI RAATTY 1 cut(s) 1056
AcuI CTGAAG 2 cut(s) 528, 719
AcyI GRCGYC 1 cut(s) 281
AdeI CACNNNGTG 2 cut(s) 154, 169
AfaI GTAC 5 cut(s) 158, 508, 606, 610, 658
AfiI CCNNNNNNNGG 7 cut(s) 110, 141, 210, 718, 809, 954, 1040
AflIII ACRYGT 1 cut(s) 409
AluBI AGCT 6 cut(s) 27, 425, 539, 809, 831, 893
AluI AGCT 6 cut(s) 27, 425, 539, 809, 831, 893
Alw21I GWGCWC 1 cut(s) 29
AlwNI CAGNNNCTG 1 cut(s) 713
Ama87I CYCGRG 1 cut(s) 197
AoxI GGCC 5 cut(s) 224, 442, 692, 709, 944
ApaI GGGCCC 1 cut(s) 696
ApeKI GCWGC 4 cut(s) 260, 425, 704, 1019
ApoI RAATTY 1 cut(s) 1056
AspS9I GGNCC 9 cut(s) 144, 225, 316, 442, 692, 693, 710, 945, 965
AsuC2I CCSGG 1 cut(s) 872
AsuHPI GGTGA 2 cut(s) 93, 791
AvaI CYCGRG 1 cut(s) 197
AvaII GGWCC 3 cut(s) 144, 316, 965
BaeGI GKGCMC 1 cut(s) 696
BanI GGYRCC 3 cut(s) 59, 65, 169
BanII GRGCYC 5 cut(s) 29, 117, 204, 639, 696
Bbv12I GWGCWC 1 cut(s) 29
BbvCI CCTCAGC 1 cut(s) 178
BbvI GCAGC 4 cut(s) 272, 412, 691, 1031
BccI CCATC 2 cut(s) 134, 656
BceAI ACGGC 1 cut(s) 959
BcgI CGANNNNNNTGC 2 cut(s) 913, 947
BclI TGATCA 2 cut(s) 883, 1051
BcnI CCSGG 1 cut(s) 872
BfaI CTAG 2 cut(s) 654, 975
BfmI CTRYAG 2 cut(s) 420, 705
BfuAI ACCTGC 2 cut(s) 709, 1001
BglI GCCNNNNNGGC 1 cut(s) 269
BisI GCNGC 6 cut(s) 72, 261, 273, 426, 705, 1020
BlsI GCNGC 6 cut(s) 73, 262, 274, 427, 706, 1021
Bme1390I CCNGG 1 cut(s) 872
Bme18I GGWCC 3 cut(s) 144, 316, 965
BmeT110I CYCGRG 1 cut(s) 197
BmgT120I GGNCC 9 cut(s) 144, 225, 316, 442, 692, 693, 710, 945, 965
BmiI GGNNCC 8 cut(s) 6, 61, 67, 145, 146, 171, 694, 695
BmrFI CCNGG 1 cut(s) 872
BmsI GCATC 4 cut(s) 107, 736, 772, 961
BpmI CTGGAG 1 cut(s) 1017
Bpu10I CCTNAGC 1 cut(s) 178
BpuMI CCSGG 1 cut(s) 872
BsaAI YACGTR 2 cut(s) 604, 608
BsaHI GRCGYC 1 cut(s) 281
BsaJI CCNNGG 3 cut(s) 322, 616, 871
BsaWI WCCGGW 1 cut(s) 244
BsaXI ACNNNNNCTCC 4 cut(s) 616, 646, 949, 979
Bsc4I CCNNNNNNNGG 7 cut(s) 110, 141, 210, 718, 809, 954, 1040
Bse118I RCCGGY 2 cut(s) 56, 62
Bse1I ACTGG 1 cut(s) 988
Bse3DI GCAATG 1 cut(s) 391
BseDI CCNNGG 3 cut(s) 322, 616, 871
BseGI GGATG 3 cut(s) 296, 442, 763
BseLI CCNNNNNNNGG 7 cut(s) 110, 141, 210, 718, 809, 954, 1040
BseMI GCAATG 1 cut(s) 391
BseMII CTCAG 4 cut(s) 14, 192, 380, 652
BseNI ACTGG 1 cut(s) 988
BseRI GAGGAG 1 cut(s) 283
BseSI GKGCMC 1 cut(s) 696
BseXI GCAGC 4 cut(s) 272, 412, 691, 1031
BseYI CCCAGC 2 cut(s) 428, 809
BsgI GTGCAG 2 cut(s) 737, 1038
Bsh1236I CGCG 1 cut(s) 206
BshFI GGCC 5 cut(s) 226, 444, 694, 711, 946
BshNI GGYRCC 3 cut(s) 59, 65, 169
BsiHKAI GWGCWC 1 cut(s) 29
BsiHKCI CYCGRG 1 cut(s) 197
BsiSI CCGG 4 cut(s) 57, 63, 245, 871
BsiWI CGTACG 1 cut(s) 604
BslFI GGGAC 2 cut(s) 130, 1058
BslI CCNNNNNNNGG 7 cut(s) 110, 141, 210, 718, 809, 954, 1040
BsmFI GGGAC 2 cut(s) 130, 1058
BsmI GAATGC 1 cut(s) 489
BsnI GGCC 5 cut(s) 226, 444, 694, 711, 946
BsoBI CYCGRG 1 cut(s) 197
Bsp120I GGGCCC 1 cut(s) 692
Bsp1286I GDGCHC 5 cut(s) 29, 117, 204, 639, 696
Bsp143I GATC 2 cut(s) 883, 1051
Bsp19I CCATGG 1 cut(s) 322
BspACI CCGC 8 cut(s) 69, 72, 182, 204, 272, 529, 678, 835
BspANI GGCC 5 cut(s) 226, 444, 694, 711, 946
BspCNI CTCAG 4 cut(s) 15, 191, 379, 651
BspFNI CGCG 1 cut(s) 206
BspLI GGNNCC 8 cut(s) 6, 61, 67, 145, 146, 171, 694, 695
BspMAI CTGCAG 1 cut(s) 709
BspMI ACCTGC 2 cut(s) 709, 1001
BspQI GCTCTTC 1 cut(s) 1000
BspT107I GGYRCC 3 cut(s) 59, 65, 169
BsrBI CCGCTC 1 cut(s) 837
BsrDI GCAATG 1 cut(s) 391
BsrFI RCCGGY 2 cut(s) 56, 62
BsrI ACTGG 1 cut(s) 988
BssAI RCCGGY 2 cut(s) 56, 62
BssECI CCNNGG 3 cut(s) 322, 616, 871
BssMI GATC 2 cut(s) 883, 1051
BssNI GRCGYC 1 cut(s) 281
BssT1I CCWWGG 2 cut(s) 322, 616
Bst4CI ACNGT 2 cut(s) 79, 421
Bst6I CTCTTC 2 cut(s) 856, 1000
BstACI GRCGYC 1 cut(s) 281
BstAPI GCANNNNNTGC 1 cut(s) 1016
BstBAI YACGTR 2 cut(s) 604, 608
BstC8I GCNNGC 6 cut(s) 204, 533, 537, 709, 891, 926
BstDEI CTNAG 5 cut(s) 23, 178, 303, 366, 638
BstDSI CCRYGG 1 cut(s) 322
BstEII GGTNACC 1 cut(s) 184
BstF5I GGATG 3 cut(s) 296, 442, 763
BstFNI CGCG 1 cut(s) 206
BstKTI GATC 2 cut(s) 886, 1054
BstMBI GATC 2 cut(s) 883, 1051
BstMWI GCNNNNNNNGC 5 cut(s) 199, 223, 260, 269, 1016
BstNSI RCATGY 3 cut(s) 413, 928, 932
BstPI GGTNACC 1 cut(s) 184
BstSCI CCNGG 1 cut(s) 870
BstSFI CTRYAG 2 cut(s) 420, 705
BstSLI GKGCMC 1 cut(s) 696
BstSNI TACGTA 2 cut(s) 604, 608
BstUI CGCG 1 cut(s) 206
BstV1I GCAGC 4 cut(s) 272, 412, 691, 1031
BsuRI GGCC 5 cut(s) 226, 444, 694, 711, 946
BtgI CCRYGG 1 cut(s) 322
BtsCI GGATG 3 cut(s) 296, 442, 763
BtsI GCAGTG 2 cut(s) 93, 762
BtsIMutI CAGTG 5 cut(s) 93, 135, 646, 762, 981
BveI ACCTGC 2 cut(s) 709, 1001
Cac8I GCNNGC 6 cut(s) 204, 533, 537, 709, 891, 926
CaiI CAGNNNCTG 1 cut(s) 713
Cfr10I RCCGGY 2 cut(s) 56, 62
Cfr13I GGNCC 9 cut(s) 144, 225, 316, 442, 692, 693, 710, 945, 965
Csp6I GTAC 5 cut(s) 157, 507, 605, 609, 657
CviAII CATG 5 cut(s) 151, 323, 410, 925, 929
CviQI GTAC 5 cut(s) 157, 507, 605, 609, 657
DdeI CTNAG 5 cut(s) 23, 178, 303, 366, 638
DpnI GATC 2 cut(s) 885, 1053
DpnII GATC 2 cut(s) 883, 1051
DraIII CACNNNGTG 2 cut(s) 154, 169
Eam1104I CTCTTC 2 cut(s) 856, 1000
EarI CTCTTC 2 cut(s) 856, 1000
EciI GGCGGA 1 cut(s) 518
Ecl136II GAGCTC 1 cut(s) 27
Eco105I TACGTA 2 cut(s) 604, 608
Eco130I CCWWGG 2 cut(s) 322, 616
Eco24I GRGCYC 5 cut(s) 29, 117, 204, 639, 696
Eco47I GGWCC 3 cut(s) 144, 316, 965
Eco53kI GAGCTC 1 cut(s) 27
Eco57I CTGAAG 2 cut(s) 528, 719
Eco88I CYCGRG 1 cut(s) 197
Eco91I GGTNACC 1 cut(s) 184
EcoICRI GAGCTC 1 cut(s) 27
EcoO109I RGGNCCY 3 cut(s) 144, 693, 710
EcoO65I GGTNACC 1 cut(s) 184
EcoT14I CCWWGG 2 cut(s) 322, 616
EcoT22I ATGCAT 2 cut(s) 491, 930
EcoT38I GRGCYC 5 cut(s) 29, 117, 204, 639, 696
ErhI CCWWGG 2 cut(s) 322, 616
FaeI CATG 5 cut(s) 154, 326, 413, 928, 932
FaqI GGGAC 2 cut(s) 130, 1058
FatI CATG 5 cut(s) 150, 322, 409, 924, 928
FauI CCCGC 1 cut(s) 211
FauNDI CATATG 1 cut(s) 562
FbaI TGATCA 2 cut(s) 883, 1051
FblI GTMKAC 1 cut(s) 353
Fnu4HI GCNGC 6 cut(s) 72, 261, 273, 426, 705, 1020
FokI GGATG 3 cut(s) 283, 449, 770
FriOI GRGCYC 5 cut(s) 29, 117, 204, 639, 696
Fsp4HI GCNGC 6 cut(s) 72, 261, 273, 426, 705, 1020
FspBI CTAG 2 cut(s) 654, 975
GluI GCNGC 6 cut(s) 72, 261, 273, 426, 705, 1020
GsaI CCCAGC 2 cut(s) 432, 813
GsuI CTGGAG 1 cut(s) 1017
HaeIII GGCC 5 cut(s) 226, 444, 694, 711, 946
HapII CCGG 4 cut(s) 57, 63, 245, 871
Hin1I GRCGYC 1 cut(s) 281
Hin1II CATG 5 cut(s) 154, 326, 413, 928, 932
HincII GTYRAC 1 cut(s) 847
HindII GTYRAC 1 cut(s) 847
HindIII AAGCTT 1 cut(s) 829
HinfI GANTC 2 cut(s) 848, 916
HpaII CCGG 4 cut(s) 57, 63, 245, 871
HphI GGTGA 2 cut(s) 93, 791
Hpy166II GTNNAC 3 cut(s) 157, 354, 847
Hpy188I TCNGA 1 cut(s) 731
Hpy188III TCNNGA 5 cut(s) 546, 855, 968, 1034, 1061
Hpy8I GTNNAC 3 cut(s) 157, 354, 847
Hpy99I CGWCG 2 cut(s) 280, 283
HpyAV CCTTC 7 cut(s) 183, 216, 262, 348, 694, 748, 948
HpyCH4III ACNGT 2 cut(s) 79, 421
HpyCH4IV ACGT 3 cut(s) 281, 603, 607
HpyCH4V TGCA 8 cut(s) 98, 389, 466, 489, 707, 718, 928, 1019
HpyF10VI GCNNNNNNNGC 5 cut(s) 199, 223, 260, 269, 1016
HpyF3I CTNAG 5 cut(s) 23, 178, 303, 366, 638
HpySE526I ACGT 3 cut(s) 281, 603, 607
Hsp92I GRCGYC 1 cut(s) 281
Hsp92II CATG 5 cut(s) 154, 326, 413, 928, 932
KflI GGGWCCC 1 cut(s) 144
Ksp22I TGATCA 2 cut(s) 883, 1051
Kzo9I GATC 2 cut(s) 883, 1051
LguI GCTCTTC 1 cut(s) 1000
LmnI GCTCC 1 cut(s) 32
Lsp1109I GCAGC 4 cut(s) 272, 412, 691, 1031
LweI GCATC 4 cut(s) 107, 736, 772, 961
MaeI CTAG 2 cut(s) 654, 975
MaeII ACGT 3 cut(s) 281, 603, 607
MaeIII GTNAC 3 cut(s) 79, 184, 760
MalI GATC 2 cut(s) 885, 1053
MbiI CCGCTC 1 cut(s) 837
MboI GATC 2 cut(s) 883, 1051
MboII GAAGA 3 cut(s) 843, 870, 1017
MhlI GDGCHC 5 cut(s) 29, 117, 204, 639, 696
MluCI AATT 3 cut(s) 450, 995, 1056
MlyI GAGTC 1 cut(s) 842
MmeI TCCRAC 2 cut(s) 61, 709
Mph1103I ATGCAT 2 cut(s) 491, 930
MseI TTAA 1 cut(s) 416
MspA1I CMGCKG 2 cut(s) 182, 809
MspI CCGG 4 cut(s) 57, 63, 245, 871
MspR9I CCNGG 1 cut(s) 872
Mva1269I GAATGC 1 cut(s) 489
MvnI CGCG 1 cut(s) 206
MwoI GCNNNNNNNGC 5 cut(s) 199, 223, 260, 269, 1016
NciI CCSGG 1 cut(s) 872
NcoI CCATGG 1 cut(s) 322
NdeI CATATG 1 cut(s) 562
NdeII GATC 2 cut(s) 883, 1051
NlaIII CATG 5 cut(s) 154, 326, 413, 928, 932
NlaIV GGNNCC 8 cut(s) 6, 61, 67, 145, 146, 171, 694, 695
NmeAIII GCCGAG 1 cut(s) 818
NmuCI GTSAC 2 cut(s) 79, 760
NsiI ATGCAT 2 cut(s) 491, 930
NspI RCATGY 3 cut(s) 413, 928, 932
PaeI GCATGC 1 cut(s) 928
PaeR7I CTCGAG 1 cut(s) 197
PciI ACATGT 1 cut(s) 409
PciSI GCTCTTC 1 cut(s) 1000
PctI GAATGC 1 cut(s) 489
PfeI GAWTC 1 cut(s) 916
Pfl23II CGTACG 1 cut(s) 604
PflFI GACNNNGTC 1 cut(s) 280
PflMI CCANNNNNTGG 3 cut(s) 809, 954, 1040
PkrI GCNGC 6 cut(s) 73, 262, 274, 427, 706, 1021
PleI GAGTC 1 cut(s) 842
PpsI GAGTC 1 cut(s) 842
Ppu21I YACGTR 2 cut(s) 604, 608
PpuMI RGGWCCY 1 cut(s) 144
PscI ACATGT 1 cut(s) 409
Psp124BI GAGCTC 1 cut(s) 29
Psp5II RGGWCCY 1 cut(s) 144
PspEI GGTNACC 1 cut(s) 184
PspFI CCCAGC 2 cut(s) 428, 809
PspLI CGTACG 1 cut(s) 604
PspN4I GGNNCC 8 cut(s) 6, 61, 67, 145, 146, 171, 694, 695
PspOMI GGGCCC 1 cut(s) 692
PspPI GGNCC 9 cut(s) 144, 225, 316, 442, 692, 693, 710, 945, 965
PspPPI RGGWCCY 1 cut(s) 144
PspXI VCTCGAGB 1 cut(s) 197
PstI CTGCAG 1 cut(s) 709
PstNI CAGNNNCTG 1 cut(s) 713
PsyI GACNNNGTC 1 cut(s) 280
PvuII CAGCTG 1 cut(s) 809
RsaI GTAC 5 cut(s) 158, 508, 606, 610, 658
RsaNI GTAC 5 cut(s) 157, 507, 605, 609, 657
SacI GAGCTC 1 cut(s) 29
SapI GCTCTTC 1 cut(s) 1000
SaqAI TTAA 1 cut(s) 416
SatI GCNGC 6 cut(s) 72, 261, 273, 426, 705, 1020
Sau3AI GATC 2 cut(s) 883, 1051
Sau96I GGNCC 9 cut(s) 144, 225, 316, 442, 692, 693, 710, 945, 965
SchI GAGTC 1 cut(s) 842
ScrFI CCNGG 1 cut(s) 872
SduI GDGCHC 5 cut(s) 29, 117, 204, 639, 696
SfaNI GCATC 4 cut(s) 107, 736, 772, 961
SfcI CTRYAG 2 cut(s) 420, 705
Sfr274I CTCGAG 1 cut(s) 197
SinI GGWCC 3 cut(s) 144, 316, 965
SlaI CTCGAG 1 cut(s) 197
SmlI CTYRAG 1 cut(s) 197
SmoI CTYRAG 1 cut(s) 197
SnaBI TACGTA 2 cut(s) 604, 608
SphI GCATGC 1 cut(s) 928
Sse9I AATT 3 cut(s) 450, 995, 1056
SsiI CCGC 8 cut(s) 69, 72, 182, 204, 272, 529, 678, 835
SspMI CTAG 2 cut(s) 654, 975
SstI GAGCTC 1 cut(s) 29
StyD4I CCNGG 1 cut(s) 870
StyI CCWWGG 2 cut(s) 322, 616
TaaI ACNGT 2 cut(s) 79, 421
TaiI ACGT 3 cut(s) 284, 606, 610
TaqI TCGA 1 cut(s) 198
TasI AATT 3 cut(s) 450, 995, 1056
TatI WGTACW 2 cut(s) 156, 656
TauI GCSGC 2 cut(s) 74, 275
TfiI GAWTC 1 cut(s) 916
Tru1I TTAA 1 cut(s) 416
Tru9I TTAA 1 cut(s) 416
TscAI CASTG 5 cut(s) 100, 142, 646, 769, 988
TseFI GTSAC 2 cut(s) 79, 760
TseI GCWGC 4 cut(s) 260, 425, 704, 1019
Tsp45I GTSAC 2 cut(s) 79, 760
TspDTI ATGAA 3 cut(s) 27, 836, 1008
TspRI CASTG 5 cut(s) 100, 142, 646, 769, 988
Tth111I GACNNNGTC 1 cut(s) 280
Van91I CCANNNNNTGG 3 cut(s) 809, 954, 1040
VpaK11BI GGWCC 3 cut(s) 144, 316, 965
XapI RAATTY 1 cut(s) 1056
XceI RCATGY 3 cut(s) 413, 928, 932
XcmI CCANNNNNNNNNTGG 1 cut(s) 137
XhoI CTCGAG 1 cut(s) 197
XmiI GTMKAC 1 cut(s) 353
XspI CTAG 2 cut(s) 654, 975
ZraI GACGTC 1 cut(s) 282
Zsp2I ATGCAT 2 cut(s) 491, 930
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.