MD12G1245000.v1.1

DNA-binding protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0011499
Physical Location & Seq
Forward (+)
31555027 .. 31559194
4168 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1245000.v1.1.491

Sequence Viewer

Length: 1062 bp
ATGGAACCGAATGAAAACCAACTGAGCTCCTACTTCCAACACCCCACCACGACGACGGCCCCCGCCGCCACTTCCACAGCCGCAACCACTCCCTCTCCAACCAATGGGCTCTTGCCCAACACCCACTCAACTTATGGGTCCCACATGTTGTACTCTCACTCGGTGCCCTCCTCGGCGGTGACCTCGCCACTTGAGCCCGCGAAAAGGAAGCGGGGGCGGCCCAGAAAGTATGGCACGCCGGAGCAGGCTCTGGCGGCCAAGAAGGCAGCGACGACGTCGTCTCACTCCTCGTCCTCCAAGGAGAAGAAGGACCAACACGGCGCCGTCTCGCCTTCGTTTTCTGGGTCGACCAAAAAATCTCAGCAGTTTTCTCTCGGCAATGCAGGGCAAGGTTTTACACCACATGTTTTAACTGTTGCTGCTGGGGAGGATGTGGGCCAGAAAATCATGTTTTTCATGCAACAGAGCAAGCGTGAAATATGCATTCTATCTGCTTCTGGTACCATCTCAAATGCATCTCTCCGCCAGCCAGCTACTTCGGGAGGCAACATTACATATGAGGGTCGCTTTGAGATTATTTCGTTGTCTGGATCTTATGTACGTACTGACCTTGGAGGGAGGGCTGGTGGGCTCAGTGTATGCCTATCTAATACAGATGGGCAAATCATCGGCGGTGGAGTTGGTGGGCCCCTGAAGGCTGCAGGCCCTGTGCAGGTTATTGTCGGAACATTTCAGATTGATGCCAAGAAGGATGCGACTGCTGGTGTAAAAGGTGATGCTTCTGCTGCCAAGTTACCATCGCCAGGGGAAATGATGAGCGGAGGCTTCCACCCAACACTCGACCCTTCTGGAAGACCTCTCGTCAGGGGGAATGAAGATCAGCAACCTATGGGGGGAAGTCACTTCATGCTTCAGGGCATGCATGTCGCACCTTCACGGCCCACAGATTGGAGAGGTGGTCCAGATGCTAGAGGCACCGGTGCTTACGAATTGACAGGAAGAGGAGTTCGTGCACCACATCAATCTCCAGAGAACGGGGACTACGATCAAATTCAAGATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF296

Protein Analysis

354

Amino Acids

36.72

Weight (kDa)

9.24

Isoelectric Point (pI)

44.13

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PCC PF03479 133 - 244 2e-23 Plants and Prokaryotes Conserved (PCC) domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0011499)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G04590 AT3G04590
fragaria_vesca FvH4_6g02360 FvH4_6g02360
malus_domestica MD00G1114400.v1.1 MD12G1245000.v1.1
prunus_persica Prupe.6G347600_v2.0.a1 Prupe.6G347600_v2.0.a1
pyrus_communis pycom04g20220 pycom12g22470
rosa_chinensis RchiOBHm_Chr3g0450121
rosa_laevigata RLG00000025754
rosa_multiflora Rmu_ssc0000116.1_g000066
rosa_roxburghii Rroxscaffold_6G00427890
rosa_rugosa Rorug02G0625700
rosa_samantha Rh3AG024900 Rh3BG025500 Rh3CG024400 Rh3DG025500
rosa_wichuraiana Rw3G001990

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 277
AatII GACGTC 1 cut(s) 278
Acc36I ACCTGC 1 cut(s) 703
Acc65I GGTACC 1 cut(s) 500
AccB1I GGYRCC 4 cut(s) 163, 320, 500, 974
AccB7I CCANNNNNTGG 2 cut(s) 104, 948
AccBSI CCGCTC 1 cut(s) 819
AccI GTMKAC 1 cut(s) 347
AccII CGCG 1 cut(s) 200
AclWI GGATC 1 cut(s) 598
AcoI YGGCCR 1 cut(s) 255
AcsI RAATTY 1 cut(s) 1050
AcuI CTGAAG 2 cut(s) 713, 896
AcyI GRCGYC 2 cut(s) 275, 321
AdeI CACNNNGTG 1 cut(s) 163
AfaI GTAC 4 cut(s) 152, 502, 600, 604
AfiI CCNNNNNNNGG 7 cut(s) 104, 204, 712, 803, 893, 948, 1034
AflIII ACRYGT 2 cut(s) 144, 403
AgeI ACCGGT 1 cut(s) 977
AgsI TTSAA 1 cut(s) 1055
AhdI GACNNNNNGTC 1 cut(s) 860
AjnI CCWGG 1 cut(s) 802
AloI GAACNNNNNNTCC 2 cut(s) 990, 1022
AluBI AGCT 2 cut(s) 27, 533
AluI AGCT 2 cut(s) 27, 533
Alw21I GWGCWC 2 cut(s) 29, 1015
Alw26I GTCTC 2 cut(s) 285, 331
Alw44I GTGCAC 1 cut(s) 1011
AlwI GGATC 1 cut(s) 598
AlwNI CAGNNNCTG 2 cut(s) 250, 707
AoxI GGCC 7 cut(s) 57, 218, 255, 436, 686, 703, 938
ApaI GGGCCC 1 cut(s) 690
ApaLI GTGCAC 1 cut(s) 1011
ApeKI GCWGC 4 cut(s) 266, 419, 698, 785
ApoI RAATTY 1 cut(s) 1050
AsiGI ACCGGT 1 cut(s) 977
Asp718I GGTACC 1 cut(s) 500
AspLEI GCGC 1 cut(s) 323
AsuHPI GGTGA 2 cut(s) 190, 785
AvaII GGWCC 3 cut(s) 138, 310, 959
BaeGI GKGCMC 3 cut(s) 168, 690, 1015
BanI GGYRCC 4 cut(s) 163, 320, 500, 974
BanII GRGCYC 5 cut(s) 29, 111, 198, 633, 690
BbsI GAAGAC 1 cut(s) 859
Bbv12I GWGCWC 2 cut(s) 29, 1015
BbvI GCAGC 4 cut(s) 278, 406, 685, 772
BccI CCATC 3 cut(s) 512, 650, 805
BceAI ACGGC 4 cut(s) 72, 308, 334, 953
BcgI CGANNNNNNTGC 2 cut(s) 907, 941
BciT130I CCWGG 1 cut(s) 804
BcoDI GTCTC 2 cut(s) 285, 331
BfaI CTAG 1 cut(s) 969
BfmI CTRYAG 1 cut(s) 699
BfoI RGCGCY 1 cut(s) 324
BfuAI ACCTGC 1 cut(s) 703
BglI GCCNNNNNGGC 1 cut(s) 263
BisI GCNGC 8 cut(s) 66, 81, 218, 255, 267, 420, 699, 786
BlsI GCNGC 8 cut(s) 67, 82, 219, 256, 268, 421, 700, 787
Bme1390I CCNGG 1 cut(s) 804
Bme18I GGWCC 3 cut(s) 138, 310, 959
BmeRI GACNNNNNGTC 1 cut(s) 860
BmrFI CCNGG 1 cut(s) 804
BmsI GCATC 5 cut(s) 524, 730, 742, 766, 955
BpiI GAAGAC 1 cut(s) 859
BpmI CTGGAG 1 cut(s) 1011
BpuEI CTTGAG 1 cut(s) 212
BsaAI YACGTR 1 cut(s) 602
BsaHI GRCGYC 2 cut(s) 275, 321
BsaJI CCNNGG 4 cut(s) 171, 297, 610, 803
BsaWI WCCGGW 1 cut(s) 977
BsaXI ACNNNNNCTCC 6 cut(s) 79, 109, 610, 640, 943, 973
Bsc4I CCNNNNNNNGG 7 cut(s) 104, 204, 712, 803, 893, 948, 1034
Bse118I RCCGGY 1 cut(s) 977
Bse3DI GCAATG 1 cut(s) 385
BseBI CCWGG 1 cut(s) 804
BseDI CCNNGG 4 cut(s) 171, 297, 610, 803
BseGI GGATG 2 cut(s) 436, 757
BseLI CCNNNNNNNGG 7 cut(s) 104, 204, 712, 803, 893, 948, 1034
BseMI GCAATG 1 cut(s) 385
BseMII CTCAG 3 cut(s) 14, 374, 646
BseRI GAGGAG 3 cut(s) 160, 277, 1017
BseSI GKGCMC 3 cut(s) 168, 690, 1015
BseXI GCAGC 4 cut(s) 278, 406, 685, 772
BseYI CCCAGC 1 cut(s) 422
BsgI GTGCAG 1 cut(s) 731
Bsh1236I CGCG 1 cut(s) 200
BshFI GGCC 7 cut(s) 59, 220, 257, 438, 688, 705, 940
BshNI GGYRCC 4 cut(s) 163, 320, 500, 974
BshTI ACCGGT 1 cut(s) 977
BsiHKAI GWGCWC 2 cut(s) 29, 1015
BsiSI CCGG 2 cut(s) 239, 978
BslFI GGGAC 2 cut(s) 124, 1052
BslI CCNNNNNNNGG 7 cut(s) 104, 204, 712, 803, 893, 948, 1034
BsmAI GTCTC 2 cut(s) 285, 331
BsmBI CGTCTC 2 cut(s) 285, 331
BsmFI GGGAC 2 cut(s) 124, 1052
BsmI GAATGC 1 cut(s) 483
BsnI GGCC 7 cut(s) 59, 220, 257, 438, 688, 705, 940
Bsp120I GGGCCC 1 cut(s) 686
Bsp1286I GDGCHC 7 cut(s) 29, 111, 168, 198, 633, 690, 1015
Bsp143I GATC 3 cut(s) 590, 877, 1045
BspANI GGCC 7 cut(s) 59, 220, 257, 438, 688, 705, 940
BspCNI CTCAG 3 cut(s) 15, 373, 645
BspFNI CGCG 1 cut(s) 200
BspMAI CTGCAG 1 cut(s) 703
BspMI ACCTGC 1 cut(s) 703
BspPI GGATC 1 cut(s) 598
BspT107I GGYRCC 4 cut(s) 163, 320, 500, 974
BsrBI CCGCTC 1 cut(s) 819
BsrDI GCAATG 1 cut(s) 385
BsrFI RCCGGY 1 cut(s) 977
BssAI RCCGGY 1 cut(s) 977
BssECI CCNNGG 4 cut(s) 171, 297, 610, 803
BssMI GATC 3 cut(s) 590, 877, 1045
BssNI GRCGYC 2 cut(s) 275, 321
BssT1I CCWWGG 2 cut(s) 297, 610
Bst2UI CCWGG 1 cut(s) 804
Bst4CI ACNGT 1 cut(s) 415
Bst6I CTCTTC 1 cut(s) 994
BstACI GRCGYC 2 cut(s) 275, 321
BstBAI YACGTR 1 cut(s) 602
BstC8I GCNNGC 8 cut(s) 198, 236, 246, 470, 527, 531, 703, 920
BstDEI CTNAG 3 cut(s) 23, 360, 632
BstEII GGTNACC 1 cut(s) 178
BstF5I GGATG 2 cut(s) 436, 757
BstFNI CGCG 1 cut(s) 200
BstH2I RGCGCY 1 cut(s) 324
BstHHI GCGC 1 cut(s) 323
BstKTI GATC 3 cut(s) 593, 880, 1048
BstMAI GTCTC 2 cut(s) 285, 331
BstMBI GATC 3 cut(s) 590, 877, 1045
BstMWI GCNNNNNNNGC 6 cut(s) 65, 193, 217, 254, 263, 785
BstNI CCWGG 1 cut(s) 804
BstNSI RCATGY 4 cut(s) 148, 407, 922, 926
BstPI GGTNACC 1 cut(s) 178
BstSCI CCNGG 1 cut(s) 802
BstSFI CTRYAG 1 cut(s) 699
BstSLI GKGCMC 3 cut(s) 168, 690, 1015
BstSNI TACGTA 1 cut(s) 602
BstUI CGCG 1 cut(s) 200
BstV1I GCAGC 4 cut(s) 278, 406, 685, 772
BstV2I GAAGAC 1 cut(s) 859
BstX2I RGATCY 1 cut(s) 590
BstYI RGATCY 1 cut(s) 590
BsuRI GGCC 7 cut(s) 59, 220, 257, 438, 688, 705, 940
BtgZI GCGATG 1 cut(s) 783
BtsCI GGATG 2 cut(s) 436, 757
BtsIMutI CAGTG 1 cut(s) 640
BveI ACCTGC 1 cut(s) 703
Cac8I GCNNGC 8 cut(s) 198, 236, 246, 470, 527, 531, 703, 920
CaiI CAGNNNCTG 2 cut(s) 250, 707
CfoI GCGC 1 cut(s) 323
Cfr10I RCCGGY 1 cut(s) 977
Csp6I GTAC 4 cut(s) 151, 501, 599, 603
CspAI ACCGGT 1 cut(s) 977
CviAII CATG 7 cut(s) 145, 404, 448, 457, 907, 919, 923
CviQI GTAC 4 cut(s) 151, 501, 599, 603
DdeI CTNAG 3 cut(s) 23, 360, 632
DinI GGCGCC 1 cut(s) 322
DpnI GATC 3 cut(s) 592, 879, 1047
DpnII GATC 3 cut(s) 590, 877, 1045
DraIII CACNNNGTG 1 cut(s) 163
DrdI GACNNNNNNGTC 1 cut(s) 277
DriI GACNNNNNGTC 1 cut(s) 860
DseDI GACNNNNNNGTC 1 cut(s) 277
EaeI YGGCCR 1 cut(s) 255
Eam1104I CTCTTC 1 cut(s) 994
Eam1105I GACNNNNNGTC 1 cut(s) 860
EarI CTCTTC 1 cut(s) 994
EciI GGCGGA 1 cut(s) 512
Ecl136II GAGCTC 1 cut(s) 27
Eco105I TACGTA 1 cut(s) 602
Eco130I CCWWGG 2 cut(s) 297, 610
Eco24I GRGCYC 5 cut(s) 29, 111, 198, 633, 690
Eco47I GGWCC 3 cut(s) 138, 310, 959
Eco53kI GAGCTC 1 cut(s) 27
Eco57I CTGAAG 2 cut(s) 713, 896
Eco91I GGTNACC 1 cut(s) 178
EcoICRI GAGCTC 1 cut(s) 27
EcoO109I RGGNCCY 3 cut(s) 138, 687, 704
EcoO65I GGTNACC 1 cut(s) 178
EcoRII CCWGG 1 cut(s) 802
EcoT14I CCWWGG 2 cut(s) 297, 610
EcoT22I ATGCAT 3 cut(s) 485, 517, 924
EcoT38I GRGCYC 5 cut(s) 29, 111, 198, 633, 690
EgeI GGCGCC 1 cut(s) 322
EheI GGCGCC 1 cut(s) 322
ErhI CCWWGG 2 cut(s) 297, 610
Esp3I CGTCTC 2 cut(s) 285, 331
FaeI CATG 7 cut(s) 148, 407, 451, 460, 910, 922, 926
FaqI GGGAC 2 cut(s) 124, 1052
FatI CATG 7 cut(s) 144, 403, 447, 456, 906, 918, 922
FauI CCCGC 3 cut(s) 70, 204, 205
FauNDI CATATG 1 cut(s) 556
FblI GTMKAC 1 cut(s) 347
Fnu4HI GCNGC 8 cut(s) 66, 81, 218, 255, 267, 420, 699, 786
FokI GGATG 2 cut(s) 443, 764
FriOI GRGCYC 5 cut(s) 29, 111, 198, 633, 690
Fsp4HI GCNGC 8 cut(s) 66, 81, 218, 255, 267, 420, 699, 786
FspBI CTAG 1 cut(s) 969
GlaI GCGC 1 cut(s) 322
GluI GCNGC 8 cut(s) 66, 81, 218, 255, 267, 420, 699, 786
GsaI CCCAGC 1 cut(s) 426
GsuI CTGGAG 1 cut(s) 1011
HaeII RGCGCY 1 cut(s) 324
HaeIII GGCC 7 cut(s) 59, 220, 257, 438, 688, 705, 940
HapII CCGG 2 cut(s) 239, 978
HhaI GCGC 1 cut(s) 323
Hin1I GRCGYC 2 cut(s) 275, 321
Hin1II CATG 7 cut(s) 148, 407, 451, 460, 910, 922, 926
Hin6I GCGC 1 cut(s) 321
HinP1I GCGC 1 cut(s) 321
HincII GTYRAC 1 cut(s) 348
HindII GTYRAC 1 cut(s) 348
HpaII CCGG 2 cut(s) 239, 978
HphI GGTGA 2 cut(s) 190, 785
Hpy166II GTNNAC 2 cut(s) 348, 1013
Hpy188I TCNGA 2 cut(s) 725, 735
Hpy188III TCNNGA 6 cut(s) 540, 588, 849, 962, 1028, 1055
Hpy8I GTNNAC 2 cut(s) 348, 1013
Hpy99I CGWCG 5 cut(s) 55, 58, 274, 277, 280
HpyAV CCTTC 7 cut(s) 256, 301, 342, 688, 742, 855, 942
HpyCH4III ACNGT 1 cut(s) 415
HpyCH4IV ACGT 2 cut(s) 275, 601
HpyCH4V TGCA 8 cut(s) 383, 460, 483, 515, 701, 712, 922, 1013
HpyF10VI GCNNNNNNNGC 6 cut(s) 65, 193, 217, 254, 263, 785
HpyF3I CTNAG 3 cut(s) 23, 360, 632
HpySE526I ACGT 2 cut(s) 275, 601
Hsp92I GRCGYC 2 cut(s) 275, 321
Hsp92II CATG 7 cut(s) 148, 407, 451, 460, 910, 922, 926
HspAI GCGC 1 cut(s) 321
KasI GGCGCC 1 cut(s) 320
KflI GGGWCCC 1 cut(s) 138
KpnI GGTACC 1 cut(s) 504
Kzo9I GATC 3 cut(s) 590, 877, 1045
LmnI GCTCC 2 cut(s) 32, 241
Lsp1109I GCAGC 4 cut(s) 278, 406, 685, 772
LweI GCATC 5 cut(s) 524, 730, 742, 766, 955
MaeI CTAG 1 cut(s) 969
MaeII ACGT 2 cut(s) 275, 601
MaeIII GTNAC 3 cut(s) 178, 792, 899
MalI GATC 3 cut(s) 592, 879, 1047
MbiI CCGCTC 1 cut(s) 819
MboI GATC 3 cut(s) 590, 877, 1045
MboII GAAGA 4 cut(s) 316, 864, 887, 1011
MflI RGATCY 1 cut(s) 590
MhlI GDGCHC 7 cut(s) 29, 111, 168, 198, 633, 690, 1015
MluCI AATT 2 cut(s) 989, 1050
Mly113I GGCGCC 1 cut(s) 321
MmeI TCCRAC 3 cut(s) 61, 122, 703
Mph1103I ATGCAT 3 cut(s) 485, 517, 924
MseI TTAA 1 cut(s) 410
MspI CCGG 2 cut(s) 239, 978
MspR9I CCNGG 1 cut(s) 804
Mva1269I GAATGC 1 cut(s) 483
MvaI CCWGG 1 cut(s) 804
MvnI CGCG 1 cut(s) 200
MwoI GCNNNNNNNGC 6 cut(s) 65, 193, 217, 254, 263, 785
NarI GGCGCC 1 cut(s) 321
NdeI CATATG 1 cut(s) 556
NdeII GATC 3 cut(s) 590, 877, 1045
NlaIII CATG 7 cut(s) 148, 407, 451, 460, 910, 922, 926
NmeAIII GCCGAG 2 cut(s) 152, 354
NmuCI GTSAC 2 cut(s) 178, 899
NsiI ATGCAT 3 cut(s) 485, 517, 924
NspI RCATGY 4 cut(s) 148, 407, 922, 926
PaeI GCATGC 1 cut(s) 922
PciI ACATGT 2 cut(s) 144, 403
PcsI WCGNNNNNNNCGW 1 cut(s) 1041
PctI GAATGC 1 cut(s) 483
PflFI GACNNNGTC 2 cut(s) 274, 277
PflMI CCANNNNNTGG 2 cut(s) 104, 948
PinAI ACCGGT 1 cut(s) 977
PkrI GCNGC 8 cut(s) 67, 82, 219, 256, 268, 421, 700, 787
PluTI GGCGCC 1 cut(s) 324
Ppu21I YACGTR 1 cut(s) 602
PpuMI RGGWCCY 1 cut(s) 138
PscI ACATGT 2 cut(s) 144, 403
Psp124BI GAGCTC 1 cut(s) 29
Psp5II RGGWCCY 1 cut(s) 138
Psp6I CCWGG 1 cut(s) 802
PspEI GGTNACC 1 cut(s) 178
PspFI CCCAGC 1 cut(s) 422
PspGI CCWGG 1 cut(s) 802
PspOMI GGGCCC 1 cut(s) 686
PspPPI RGGWCCY 1 cut(s) 138
PsrI GAACNNNNNNTAC 2 cut(s) 1025, 1057
PstI CTGCAG 1 cut(s) 703
PstNI CAGNNNCTG 2 cut(s) 250, 707
PsuI RGATCY 1 cut(s) 590
PsyI GACNNNGTC 2 cut(s) 274, 277
RsaI GTAC 4 cut(s) 152, 502, 600, 604
RsaNI GTAC 4 cut(s) 151, 501, 599, 603
SacI GAGCTC 1 cut(s) 29
SalI GTCGAC 1 cut(s) 346
SaqAI TTAA 1 cut(s) 410
SatI GCNGC 8 cut(s) 66, 81, 218, 255, 267, 420, 699, 786
Sau3AI GATC 3 cut(s) 590, 877, 1045
ScrFI CCNGG 1 cut(s) 804
SduI GDGCHC 7 cut(s) 29, 111, 168, 198, 633, 690, 1015
SfaNI GCATC 5 cut(s) 524, 730, 742, 766, 955
SfcI CTRYAG 1 cut(s) 699
SfoI GGCGCC 1 cut(s) 322
SgrAI CRCCGGYG 1 cut(s) 977
SinI GGWCC 3 cut(s) 138, 310, 959
SmlI CTYRAG 1 cut(s) 191
SmoI CTYRAG 1 cut(s) 191
SnaBI TACGTA 1 cut(s) 602
SphI GCATGC 1 cut(s) 922
Sse9I AATT 2 cut(s) 989, 1050
SspDI GGCGCC 1 cut(s) 320
SspMI CTAG 1 cut(s) 969
SstI GAGCTC 1 cut(s) 29
StyD4I CCNGG 1 cut(s) 802
StyI CCWWGG 2 cut(s) 297, 610
TaaI ACNGT 1 cut(s) 415
TaiI ACGT 2 cut(s) 278, 604
TaqI TCGA 2 cut(s) 347, 840
TasI AATT 2 cut(s) 989, 1050
TatI WGTACW 1 cut(s) 150
TauI GCSGC 4 cut(s) 68, 83, 220, 257
Tru1I TTAA 1 cut(s) 410
Tru9I TTAA 1 cut(s) 410
TscAI CASTG 1 cut(s) 640
TseFI GTSAC 2 cut(s) 178, 899
TseI GCWGC 4 cut(s) 266, 419, 698, 785
Tsp45I GTSAC 2 cut(s) 178, 899
TspDTI ATGAA 4 cut(s) 27, 445, 888, 895
TspRI CASTG 1 cut(s) 640
Tth111I GACNNNGTC 2 cut(s) 274, 277
Van91I CCANNNNNTGG 2 cut(s) 104, 948
VneI GTGCAC 1 cut(s) 1011
VpaK11BI GGWCC 3 cut(s) 138, 310, 959
XapI RAATTY 1 cut(s) 1050
XceI RCATGY 4 cut(s) 148, 407, 922, 926
XcmI CCANNNNNNNNNTGG 1 cut(s) 131
XmiI GTMKAC 1 cut(s) 347
XspI CTAG 1 cut(s) 969
ZraI GACGTC 1 cut(s) 276
Zsp2I ATGCAT 3 cut(s) 485, 517, 924
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.