AT3G05680

Protein virilizer homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
3
OG0010141
Physical Location & Seq
Reverse (-)
1660523 .. 1672608
12086 bp
Loading structure...
UTR
Exon/CDS
Intron
AT3G05680.2

Sequence Viewer

Length: 6417 bp
ATGGTACGATCTGAGCCTTGTGTTCTGTTCGCTCAGACCTTTGTTCATCCTCAATTGGACGAATATGTTGACGAGGTGATATTTGCTGAGCCAGTAATCATCACAGCTTGCGAATTTTTGGAGCAGAATGCTTCCTCATCTTCTCAAGCTGTTTCTCTTGTCGGGGCAACTTCACCTCCTTCATTTGCGTTGGAAGTGTTCGTTCGATGTGAAGGAGAATCAAAGTTTAAGCGACTTTGTAATCCTTTTCTTTACACTCCTTCAGCGCCCTACCCTTTGGAAGTGGAGGCTGTTGTAACAAACCATCTGGTGGTCAGAGGTAGCTATCGAAGTTTGAGCTTGATCGTCTATGGGAACATCGTCAAAGATTTGGGGCAGTACAATATCATTCTTGAAGGTCGTTCTGTAACTGATATTGTGAGTTCCACTGAGGGTAATCTTGAGGACTTGCCATTGGTATTGCACTCAGTTAACAGAACAATAGAGGAATGTCTATCCTCTCTAGACATAGTATCTCTCCCCCTGGCTGCTGTAGATTTGCCGGTGGAAGTAAAGCGATTGTTGCAGCTGCTGCTAAAAATCTTTGATAAGCTAGCTACTAATGATGTAGTAAATAAATTTGTGGATACGGTAGTGTCTGGGGTCTCCTCGTATGTCACAGACAATGTAGATTTCTTTTTAAAGAATAAGAATTGTTCGGCTGTGACCAGTTCCCTAGATTCTGGGTTATTCCATGATATAGTTGACAGGGTTAAGGAAGATATTCTTGATCTCAATGAAATTCAAGAATCAGATGTGGCACTTGGATTGTTCAGCTTTCTTGAATCAGAGACTTATTTGGCTACTTCCCAACAATTGGTTGTTATGTTGAGCCCATATATTCAGTTTGAAAGAGATTCCTTATGTACTGTCCTTCCAAAACTTTCAAAGGGGAAAGCCACTCTTTTAGGTTTAAGTTTGGCCTTCTTATTATGCTCTGGCCGAGAAGGCTGCCTCCAATTTGTCAATTCGGGTGGGATGGATCAACTTGTATATTTGTTTGGTCATGATGGTCAGAATTCTACCACCATTACTTTGCTGTTGCTTGGAGTTGTTGAGCAGGCTACACGCCATTCAGTTGGGTGTGAAGGATTTTTAGGTTGGTGGCCTCGTGAGGATGGAAGTATCCCATCTGGTAAGAGTGAGGGCTACTGTCTTCTGTTGAAATTACTTATGCAGAAACCATGCCATGAGATTGCCTCTCTTGCAATTTATATTCTTCGTCGTTTGAGAATTTACGAGGTTATATCAAGATATGAGTTTGCAGTGTTGTCAGCACTGGAAGGTCTCTCTAATTCTCATGGGGCTGCAACTCATAATCTGAATATGCTATCTGATGCTAAGTCACAACTTCAAAAGCTCCAGAACCTGATGAAGTCACTTGGATCAGTTGAAGATCCCTCACCATCAGCCTACGCAGAAAGAAGTTTGGTTTCTGATCATTCTGAAGGGTGGTTGTCATACAAAGCCACAAGTAAACTAACTTCTTCATGGACATGTCCCTTTTATAGCTCTGGAATTGATTCGCACATATTGGCACTTCTGAAGGAGCGGGGTTTTCTTCCTTTGTCAGCAGCGCTTCTCTCAATGCCTGAATTGCATTCTAAAGTTGGAGATATTATGGATGTCTTTACTGATATCGCAATGTTTATTGGGAACATAATTCTTTCATTTATGTTTTCACGCACAGGATTGAGCTTTTTGCTACATCATCCTGAACTGACTGCTACAATAATACAATCTCTGAAGGGATCCGTCGATCTGAACAAGGAGGAGTGTGTCCCCCTCCACTATGCTTCAATTTTAATATCCAAGGGTTTTACTTGCAGCTTACTGGAAATTGGAATAAATCTTGAGATGCATTTGAGGGTGGTGAGTGCAGTAGATCGTTTGCTCAAGTCGATACAGCAAACAGAAGAATTTCTATGGATTTTATGGGAGTTGCGTGATGTTTCTAGGTCTGATTGTGGGCGTGAAGCTTTATTGACGTTAGGTGTTTTTCCAGAGGCTTTAGCAGTCTTGATTGAAGCTTTGCATTCAGCTAAGGATATGGAGCCGGCAGTTGAAAATAGTGGAATTTCACCGCTTAATCTAGCAATATGTCACTCAGCTGCTGAGATTTTTGAGGTCATAGTCTCTGACTCAACTGCATCATGCTTGCATGCTTGGATTGAACATGCCCCAGTCCTTCATAAGGCCTTGCATACTTTGTCACCGGGTGGTTCTAATCGAAAAGATGCTCCTTCACGATTACTTAAATGGATTGATGCGGGTGTGGTTTATCACAAACATGGCGTAGGTGGCCTTTTACGTTATGCTGCTGTCTTGGCTTCTGGAGGGGACGCCCAATTATCATCATCAAGCATCCTTGCACTCGATTTAACACCGGCTGAGAATGGTGCTGGAGAATCAACAAATGTCTCTGAGATGAACGTCCTAGATAATCTTGGAAAAGTTATCTTTGAGAAATCTTTTGAAGGTGTTAACCTTTCTGATTCATCAATTTCACAGTTGACAACAGCACTTCGAATTTTAGCGCTGATTTCAGATAATTCAACTGTTGCTGCTGCTTTATATGACGAGGGTGCGGTTACAGTTGTGTATGCTATCTTGGTCAACTGCAGCTTTATGTTTGAGAGGTCGTCAAATATTTATGATTATCTGGTGGATGACGATCATGGATGCAGTTCTATTTCTGACTTTTTGTCTGAACGTAATCGTGAGCAAAGCTTGGTGGACCTGTTAATTCCTTCCCTTGTCCTCTTGATATCAGTCCTACAAAGACTACAGGGAACTAAGGAGCAGTACAGAAACACCAAATTGATGAAGGCACTCCTAAGGCTACATCGGGAAGTCAGCCCGAAGCTAGCTGCTTGTGCAGCTGACTTGTCCTCCCATTATCCAGACTCTGCTCTTGGCTTTGGAGCAGTGTGCCACCTCATTGTGTCCGCCCTTGTCTGCTGGCCAGTTTATGGATGGATTCCTGGGCTATTCCACACTCTTCTTTCGGGTGTCCAGACGTCCTCTGTTCCTGCCTTGGGTCCAAAGGAGACCTGTAGCTTCCTCTGCATTCTAAGCGATATTCTCCCCGAAGAAGGAGTCTGGTTTTGGAAGAGTGGCATGCCCTTATTAAGTGGTCTAAGAAAGTTGGCGGTTGGGACATTGATGGGTCCACAGAAGGAGAAACAGATCAACTGGTATCTGGAGCCTGGTCCACTTGAAAAGCTGATAAACCATTTGACACCTAATCTTGACAAGATTGCAAAGATTATCCAACATCATGCAGTATCTGCATTGGTGGTCATTCAGGATATGCTTCGGGTTTTCATTGTTCGTATTGCATGTCAAAGGGTTGAACATGCTTCTATCCTTCTGAGGCCCATATTTTCATCTATCAGAGATGGAATTTTGGATCAGTCATCAACAAGAGACACTGAAGCTTATATGGTCTACCGATATCTTAATTTTCTGGCTAGTTTATTAGAGCATCCGCATGCTAAGGGATTACTGTTAGAGGAGGGTATTGTTCAACTGCTTGTGGAAGTTCTGGAAAGATGTTATGATGCCACTTATCCAAGTGAAAACAGGGTTCTAGAGTATGGAATTGTGTCCGCGTCTAGTGTGATTCAGTGGTGTATTCCAGCTTTCAGATCGATCTCACTGCTATGCGATTCACAAGTGCCTCTCTTGTGCTTTCAGAAAAAAGAGCTATTGGCCAGTCTGAGTGCAAAAGATTGTGCATTGATATTTCCCTTTGTGCTGAAGTTCTGCCAGGTGTTGCCTGTTGGCAATGAGCTATTATCTTGTCTTGGCGCTTTCAAAGACCTGAGCTCATGTGGTGAAGGTCAAGATGGTCTGGTTTCACTTTTGTTTCATCTGTTTTCTGGTACCGAAGAATCTGTATCTGAAAGGTGGTGTGACACTAATAGTCTCAGTCTCGATCAACTAGATATGAAGAAAAATCCCCCTTTTCTGTCTTGCTGGATCAAGTTATTGAACTCAATTAATTCAAAAGATGGTTTGTCTAGTCTTGCAATGAAGGCAGTAAACGTTCTGAGCGTGGGCTCCATCAGATTATGCCTTGATGGTAAAAGTTTGGACTCGAAGAAGGTTGCAGCACTAAAATCTCTTTTTGGCCTTCCAAGTGAGTTTAGTGGTACAGACACTTTTAGAGAGGAAAACATTGGTTTGATTGAACAGATGGTGACACTGTTGAGTTCAATGACTTCGGGGTCTGATAGTTCCGCAACAGCTGAGATGAAACCCTATTTGCATGAGGCATCTCAGTCGTTGCTGTCGTTGCTAAAAGACGGGAATATTGATGATATTATCTCCTGCAAAGGTGTTTTTGTTTCTCCTGGGAATCTTGATATGGATGATTTGGTTTCTAGAAACATTGAAGATGATCTCTATCAAAGAGGACTTGAAGACAAGTTCTGGTGGGAATGTCCAGAAACATTACCTGAGCGTCTGCCCCAGTCATCTCTCCCTGCGAAAAGGAAACTACCAACTTTGGAGAGCTCTAGCAGGCGTGCTAAAGGCGAAAACTCTTCTGTTGACATACCTACCCAAAATTCAATTCAACGGGGAATGGGTTCTGTATCCCTTCCTCCTGCTCCTACACGTAGAGATGCCTTTAGGCAGCGCAAGCCGAATACCAGCAGGCCTCCCTCTATGCATGTAGATGATTATGTTGCTAGGGAAAGAAGTGTTGATACAGCTGGTAATTCTAATGCAATAACCATTTCACGAGCAGGATCTAGCAGTGGTAGACCTCCATCTATTCATGTTGATGAGTTTATGGCCAGACAGAGAGAACGAGGCCAAAATCCCTCCACCATTGTGGTTGGAGAGGCTGTTGTGCAAGTGAAAAATCCTACTCCTGCTAGAGATACTGAAAAAGTTGCAGGTAAACCTAAACAATTCAAAGCCGATCCTGATGATGATCTACAAGGAATAGATATAGTTTTTGATGGTGAGGAATGTGAAGGACCTGATGACAAGTTGCCCTTTCTTCAGCCCGATGAGAACCTTATGCAGCCTGCTCCTGTCATGGTTGAACAAAACTCTCCTCACTCTATTGTTGAAGAAACTGAGAGTGATGCAAATGGAAGTAGTCAATTCTCTCACATGGGCACACCAGTGGCATCTAATGTTGACGAAAACGCTCAGAGTGAATTTTCATCAAGGATTTCAGTTTCACGCCCTGAGATGTCGTTGATTCGGGAACCAAGCATTTCTTCAGACAGAAAATTTGTTGAGCAAGCAGACGAAGCAAAGAAAATGGCCCCTCTAAAGAGTGCCGGCATATCTGAATCTGGGTTTATACCTGCTTATCACATGCCAGGATCTTCAGGACAAAATTCAATTGATCCTAGAGTGGGTCCTCAGGGTTTCTATTCAAAGAGTGGTCAGCAACATACGGGCCATATTCATGGTGGATTCAGTGGTCGGGGGGTTTATGAACAGAAAGTGATGCCAAACCAACCTCCTTTACCCTTAGTGCCACCTCCATCAGTCTCACCTGTGATACCACATTCTTCTGATTCTCTTTCTAATCAATCCTCCCCGTTTATTAGTCATGGAACACAATCTTCAGGAGGACCCACCCGGCTTATGCCACCGCTCCCTTCAGCAATTCCTCAGTACTCATCTAACCCATATGCATCTTTACCACCAAGGACATCTACAGTTCAATCTTTTGGATACAACCACGCAGGCGTAGGTACCACTGAACAACAACAGAGTGGCCCAACCATTGATCATCAGTCTGGCAATCTATCTGTTACCGGAATGACGTCTTACCCTCCCCCAAATTTAATGCCATCCCATAATTTTAGCAGGCCATCATCTCTCCCTGTGCCATTCTATGGAAATCCTTCGCATCAAGGAGGTGATAAGCCGCAAACTATGTTGTTGGTTCCTTCCATTCCTCAATCACTCAATACACAGTCCATCCCTCAGCTGCCATCGATGCAGCTTTCACAATTACAACGCCCTATGCAGCCTCCTCAGCACGTTAGACCACCCATACAGATATCTCAACCATCAGAGCAAGGAGTATCAATGCAAAACCCATTTCAGATTCCAATGCATCAGATGCAGCTGATGCAACAGACGCAAGTCCAGCCTTACTATCACCCTCCTCAACAACAAGAGATCTCCCAGGTGCAGCAACAGCAACAGCATCATGCTGTTCAAGGTCAGCAAGGAGCTGGGACTTCCCAGCAACAAGAATCTGGAATGTCATTACATGATTACTTCAAGTCCCCTGAAGCCATTCAGGCTCTATTAAGTGACCGTGACAAGCTGTGTCAGCTACTGGAACAACATCCAAAGTTAATGCAGATGCTTCAGGAAAAACTTGGGCAGCTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
VIR_N

Protein Analysis

2138

Amino Acids

234.22

Weight (kDa)

5.39

Isoelectric Point (pI)

50.86

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
VIR_N PF15912 8 - 123 6.1e-07 Virilizer, N-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0010141)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G05680 AT3G05680
fragaria_vesca FvH4_6g20410 FvH4_6g20410 FvH4_6g20410
malus_domestica MD12G1041200.v1.1 MD12G1041400.v1.1 MD14G1040500.v1.1
prunus_persica Prupe.7G093800_v2.0.a1
pyrus_communis pycom12g03590 pycom14g03440
rosa_chinensis RchiOBHm_Chr3g0476141
rosa_laevigata RLG00000023788
rosa_multiflora Rmu_sc0000114.1_g000025
rosa_roxburghii Rroxscaffold_6G00405450
rosa_rugosa Rorug03G0153000
rosa_samantha Rh3AG204100 Rh3BG234300 Rh3CG229700 Rh3DG229700
rosa_wichuraiana Rw3G018550

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 4270
AatII GACGTC 2 cut(s) 3062, 5840
Acc36I ACCTGC 2 cut(s) 4937, 5377
Acc65I GGTACC 2 cut(s) 3926, 5765
AccB1I GGYRCC 2 cut(s) 3926, 5765
AccB7I CCANNNNNTGG 4 cut(s) 310, 856, 3011, 5909
AccBSI CCGCTC 2 cut(s) 1591, 5665
AccI GTMKAC 2 cut(s) 3489, 4810
AccII CGCG 1 cut(s) 3653
AclI AACGTT 1 cut(s) 4089
AcoI YGGCCR 4 cut(s) 979, 3002, 3753, 4842
AcyI GRCGYC 3 cut(s) 2382, 3059, 5837
AdeI CACNNNGTG 1 cut(s) 2258
AfaI GTAC 8 cut(s) 6, 380, 907, 2846, 3928, 4198, 5687, 5767
AfeI AGCGCT 2 cut(s) 1617, 2577
AfiI CCNNNNNNNGG 8 cut(s) 310, 856, 2233, 3011, 3077, 3134, 5420, 5909
AflIII ACRYGT 2 cut(s) 1535, 4661
AhdI GACNNNNNGTC 1 cut(s) 2743
AjnI CCWGG 7 cut(s) 522, 3022, 3247, 3810, 4396, 5383, 6204
AjuI GAANNNNNNNTTGG 4 cut(s) 4602, 4634, 5263, 5295
AleI CACNNNNGTG 2 cut(s) 4880, 5180
Alw21I GWGCWC 2 cut(s) 3872, 4562
AlwNI CAGNNNCTG 6 cut(s) 571, 1408, 2153, 2948, 3329, 6361
Aor51HI AGCGCT 2 cut(s) 1617, 2577
ArsI GACNNNNNNTTYG 4 cut(s) 3123, 3155, 5276, 5308
AseI ATTAAT 1 cut(s) 4044
Asp700I GAANNNNTTC 6 cut(s) 762, 1561, 1959, 4633, 5917, 6318
Asp718I GGTACC 2 cut(s) 3926, 5765
AspLEI GCGC 5 cut(s) 268, 1618, 2578, 3854, 4686
AsuC2I CCSGG 2 cut(s) 2256, 5650
AsuII TTCGAA 1 cut(s) 2566
AsuNHI GCTAGC 2 cut(s) 592, 2905
AvaII GGWCC 7 cut(s) 2776, 3080, 3209, 3251, 5030, 5423, 5642
AxyI CCTNAGG 2 cut(s) 2876, 5427
BaeGI GKGCMC 1 cut(s) 5177
BaeI ACNNNNGTAYC 2 cut(s) 618, 651
BalI TGGCCA 3 cut(s) 3004, 3755, 4844
BamHI GGATCC 1 cut(s) 1790
BanI GGYRCC 2 cut(s) 3926, 5765
BanII GRGCYC 4 cut(s) 875, 3872, 4106, 4562
BarI GAAGNNNNNNTAC 4 cut(s) 1507, 1539, 4738, 4770
BauI CACGAG 2 cut(s) 1149, 4788
BbsI GAAGAC 2 cut(s) 1187, 4473
Bbv12I GWGCWC 2 cut(s) 3872, 4562
BbvCI CCTCAGC 2 cut(s) 6000, 6051
BcgI CGANNNNNNTGC 6 cut(s) 972, 1006, 3682, 3716, 4308, 4342
BciT130I CCWGG 7 cut(s) 524, 3024, 3249, 3812, 4398, 5385, 6206
BciVI GTATCC 4 cut(s) 619, 1175, 4651, 5738
BclI TGATCA 2 cut(s) 1477, 5800
BcnI CCSGG 2 cut(s) 2256, 5650
BfmI CTRYAG 5 cut(s) 531, 2659, 2825, 3094, 5727
BfoI RGCGCY 4 cut(s) 269, 1619, 2579, 3855
BfuAI ACCTGC 2 cut(s) 4937, 5377
BfuI GTATCC 4 cut(s) 619, 1175, 4651, 5738
BglI GCCNNNNNGGC 2 cut(s) 987, 6323
BglII AGATCT 1 cut(s) 6198
BlpI GCTNAGC 1 cut(s) 87
BmcAI AGTACT 1 cut(s) 5687
Bme1390I CCNGG 9 cut(s) 524, 2256, 3024, 3249, 3812, 4398, 5385, 5650, 6206
Bme18I GGWCC 7 cut(s) 2776, 3080, 3209, 3251, 5030, 5423, 5642
BmeRI GACNNNNNGTC 1 cut(s) 2743
BmrFI CCNGG 9 cut(s) 524, 2256, 3024, 3249, 3812, 4398, 5385, 5650, 6206
BmrI ACTGGG 2 cut(s) 2216, 4510
BmtI GCTAGC 2 cut(s) 596, 2909
BmuI ACTGGG 2 cut(s) 2216, 4510
BoxI GACNNNNGTC 1 cut(s) 6161
BpiI GAAGAC 2 cut(s) 1187, 4473
BplI GAGNNNNNCTC 2 cut(s) 1223, 1255
BpmI CTGGAG 4 cut(s) 1385, 2394, 2463, 3263
Bpu10I CCTNAGC 6 cut(s) 2082, 3537, 3866, 4503, 6000, 6051
Bpu1102I GCTNAGC 1 cut(s) 87
Bpu14I TTCGAA 1 cut(s) 2566
BpuEI CTTGAG 4 cut(s) 129, 461, 1913, 1919
BpuMI CCSGG 2 cut(s) 2256, 5650
Bsa29I ATCGAT 2 cut(s) 3692, 6011
BsaAI YACGTR 1 cut(s) 4664
BsaBI GATNNNNATC 3 cut(s) 2712, 4449, 4983
BsaHI GRCGYC 3 cut(s) 2382, 3059, 5837
BsaI GGTCTC 3 cut(s) 649, 1331, 3083
BsaJI CCNNGG 7 cut(s) 522, 1851, 3023, 3075, 4397, 5717, 6204
BsaWI WCCGGW 1 cut(s) 5828
BsaXI ACNNNNNCTCC 6 cut(s) 160, 190, 2915, 2945, 4881, 4911
Bsc4I CCNNNNNNNGG 8 cut(s) 310, 856, 2233, 3011, 3077, 3134, 5420, 5909
Bse118I RCCGGY 4 cut(s) 541, 2095, 2425, 5342
Bse21I CCTNAGG 2 cut(s) 2876, 5427
Bse3DI GCAATG 3 cut(s) 1689, 3835, 4080
Bse8I GATNNNNATC 3 cut(s) 2712, 4449, 4983
BseBI CCWGG 7 cut(s) 524, 3024, 3249, 3812, 4398, 5385, 6206
BseCI ATCGAT 2 cut(s) 3692, 6011
BseDI CCNNGG 7 cut(s) 522, 1851, 3023, 3075, 4397, 5717, 6204
BseJI GATNNNNATC 3 cut(s) 2712, 4449, 4983
BseLI CCNNNNNNNGG 8 cut(s) 310, 856, 2233, 3011, 3077, 3134, 5420, 5909
BseMI GCAATG 3 cut(s) 1689, 3835, 4080
BseRI GAGGAG 6 cut(s) 637, 1826, 3569, 5100, 6039, 6174
BseSI GKGCMC 1 cut(s) 5177
BseYI CCCAGC 2 cut(s) 6254, 6264
BsgI GTGCAG 3 cut(s) 1938, 2937, 6230
Bsh1236I CGCG 1 cut(s) 3653
BshNI GGYRCC 2 cut(s) 3926, 5765
BshVI ATCGAT 2 cut(s) 3692, 6011
BsiHKAI GWGCWC 2 cut(s) 3872, 4562
BsiSI CCGG 7 cut(s) 542, 2096, 2255, 2426, 5343, 5650, 5829
BslFI GGGAC 6 cut(s) 1524, 1805, 2393, 3211, 6271, 6292
BslI CCNNNNNNNGG 8 cut(s) 310, 856, 2233, 3011, 3077, 3134, 5420, 5909
BsmFI GGGAC 6 cut(s) 1524, 1805, 2393, 3211, 6271, 6292
BsmI GAATGC 4 cut(s) 133, 1639, 2074, 3108
Bso31I GGTCTC 3 cut(s) 649, 1331, 3083
Bsp119I TTCGAA 1 cut(s) 2566
Bsp1286I GDGCHC 5 cut(s) 875, 3872, 4106, 4562, 5177
Bsp1720I GCTNAGC 1 cut(s) 87
BspDI ATCGAT 2 cut(s) 3692, 6011
BspFNI CGCG 1 cut(s) 3653
BspHI TCATGA 1 cut(s) 1045
BspMAI CTGCAG 1 cut(s) 2663
BspMI ACCTGC 2 cut(s) 4937, 5377
BspOI GCTAGC 2 cut(s) 596, 2909
BspT104I TTCGAA 1 cut(s) 2566
BspT107I GGYRCC 2 cut(s) 3926, 5765
BspTNI GGTCTC 3 cut(s) 649, 1331, 3083
BsrBI CCGCTC 2 cut(s) 1591, 5665
BsrDI GCAATG 3 cut(s) 1689, 3835, 4080
BsrFI RCCGGY 4 cut(s) 541, 2095, 2425, 5342
BssAI RCCGGY 4 cut(s) 541, 2095, 2425, 5342
BssECI CCNNGG 7 cut(s) 522, 1851, 3023, 3075, 4397, 5717, 6204
BssNI GRCGYC 3 cut(s) 2382, 3059, 5837
BssSI CACGAG 2 cut(s) 1149, 4788
BssT1I CCWWGG 3 cut(s) 1851, 3075, 5717
Bst2BI CACGAG 2 cut(s) 1149, 4788
Bst2UI CCWGG 7 cut(s) 524, 3024, 3249, 3812, 4398, 5385, 6206
Bst6I CTCTTC 3 cut(s) 3045, 3146, 4594
BstACI GRCGYC 3 cut(s) 2382, 3059, 5837
BstAPI GCANNNNNTGC 4 cut(s) 571, 3329, 6139, 6148
BstBAI YACGTR 1 cut(s) 4664
BstBI TTCGAA 1 cut(s) 2566
BstENI CCTNNNNNAGG 1 cut(s) 2231
BstFNI CGCG 1 cut(s) 3653
BstH2I RGCGCY 4 cut(s) 269, 1619, 2579, 3855
BstHHI GCGC 5 cut(s) 268, 1618, 2578, 3854, 4686
BstNI CCWGG 7 cut(s) 524, 3024, 3249, 3812, 4398, 5385, 6206
BstNSI RCATGY 9 cut(s) 1539, 2204, 2219, 3163, 3384, 3401, 3536, 4721, 5383
BstPAI GACNNNNGTC 1 cut(s) 6161
BstSCI CCNGG 9 cut(s) 522, 2254, 3022, 3247, 3810, 4396, 5383, 5648, 6204
BstSFI CTRYAG 5 cut(s) 531, 2659, 2825, 3094, 5727
BstSLI GKGCMC 1 cut(s) 5177
BstUI CGCG 1 cut(s) 3653
BstV2I GAAGAC 2 cut(s) 1187, 4473
BstX2I RGATCY 5 cut(s) 1435, 1790, 4796, 5387, 6198
BstXI CCANNNNNNTGG 3 cut(s) 1118, 4882, 5474
BstYI RGATCY 5 cut(s) 1435, 1790, 4796, 5387, 6198
Bsu15I ATCGAT 2 cut(s) 3692, 6011
Bsu36I CCTNAGG 2 cut(s) 2876, 5427
BsuI GTATCC 4 cut(s) 619, 1175, 4651, 5738
BsuTUI ATCGAT 2 cut(s) 3692, 6011
BtsI GCAGTG 4 cut(s) 1311, 2973, 3698, 4810
BveI ACCTGC 2 cut(s) 4937, 5377
CaiI CAGNNNCTG 6 cut(s) 571, 1408, 2153, 2948, 3329, 6361
CciI TCATGA 1 cut(s) 1045
CfoI GCGC 5 cut(s) 268, 1618, 2578, 3854, 4686
Cfr10I RCCGGY 4 cut(s) 541, 2095, 2425, 5342
ClaI ATCGAT 2 cut(s) 3692, 6011
CseI GACGC 4 cut(s) 2390, 3642, 4496, 6166
Csp6I GTAC 8 cut(s) 5, 379, 906, 2845, 3927, 4197, 5686, 5766
CspCI CAANNNNNGTGG 2 cut(s) 994, 1029
CviQI GTAC 8 cut(s) 5, 379, 906, 2845, 3927, 4197, 5686, 5766
DraI TTTAAA 1 cut(s) 681
DraIII CACNNNGTG 1 cut(s) 2258
DrdI GACNNNNNNGTC 1 cut(s) 4270
DriI GACNNNNNGTC 1 cut(s) 2743
DseDI GACNNNNNNGTC 1 cut(s) 4270
EaeI YGGCCR 4 cut(s) 979, 3002, 3753, 4842
Eam1104I CTCTTC 3 cut(s) 3045, 3146, 4594
Eam1105I GACNNNNNGTC 1 cut(s) 2743
EarI CTCTTC 3 cut(s) 3045, 3146, 4594
EciI GGCGGA 1 cut(s) 2977
Ecl136II GAGCTC 2 cut(s) 3870, 4560
Eco130I CCWWGG 3 cut(s) 1851, 3075, 5717
Eco147I AGGCCT 2 cut(s) 2237, 4705
Eco24I GRGCYC 4 cut(s) 875, 3872, 4106, 4562
Eco31I GGTCTC 3 cut(s) 649, 1331, 3083
Eco32I GATATC 4 cut(s) 1678, 2808, 3497, 6078
Eco47I GGWCC 7 cut(s) 2776, 3080, 3209, 3251, 5030, 5423, 5642
Eco47III AGCGCT 2 cut(s) 1617, 2577
Eco53kI GAGCTC 2 cut(s) 3870, 4560
Eco81I CCTNAGG 2 cut(s) 2876, 5427
EcoICRI GAGCTC 2 cut(s) 3870, 4560
EcoNI CCTNNNNNAGG 1 cut(s) 2231
EcoO109I RGGNCCY 3 cut(s) 5030, 5423, 5642
EcoRI GAATTC 1 cut(s) 1057
EcoRII CCWGG 7 cut(s) 522, 3022, 3247, 3810, 4396, 5383, 6204
EcoRV GATATC 4 cut(s) 1678, 2808, 3497, 6078
EcoT14I CCWWGG 3 cut(s) 1851, 3075, 5717
EcoT22I ATGCAT 4 cut(s) 1902, 4719, 5707, 6135
EcoT38I GRGCYC 4 cut(s) 875, 3872, 4106, 4562
ErhI CCWWGG 3 cut(s) 1851, 3075, 5717
FalI AAGNNNNNCTT 8 cut(s) 750, 782, 927, 959, 5033, 5065, 5263, 5295
FaqI GGGAC 6 cut(s) 1524, 1805, 2393, 3211, 6271, 6292
FauI CCCGC 2 cut(s) 1584, 2302
FauNDI CATATG 1 cut(s) 5701
FbaI TGATCA 2 cut(s) 1477, 5800
FblI GTMKAC 2 cut(s) 3489, 4810
FriOI GRGCYC 4 cut(s) 875, 3872, 4106, 4562
GlaI GCGC 5 cut(s) 267, 1617, 2577, 3853, 4685
GsaI CCCAGC 2 cut(s) 6258, 6268
GsuI CTGGAG 4 cut(s) 1385, 2394, 2463, 3263
HaeII RGCGCY 4 cut(s) 269, 1619, 2579, 3855
HapII CCGG 7 cut(s) 542, 2096, 2255, 2426, 5343, 5650, 5829
HgaI GACGC 4 cut(s) 2390, 3642, 4496, 6166
HhaI GCGC 5 cut(s) 268, 1618, 2578, 3854, 4686
Hin1I GRCGYC 3 cut(s) 2382, 3059, 5837
Hin6I GCGC 5 cut(s) 266, 1616, 2576, 3852, 4684
HinP1I GCGC 5 cut(s) 266, 1616, 2576, 3852, 4684
HincII GTYRAC 8 cut(s) 70, 472, 745, 2524, 2553, 2656, 4597, 5197
HindII GTYRAC 8 cut(s) 70, 472, 745, 2524, 2553, 2656, 4597, 5197
HindIII AAGCTT 4 cut(s) 2016, 2067, 2767, 3477
HpaI GTTAAC 2 cut(s) 472, 2524
HpaII CCGG 7 cut(s) 542, 2096, 2255, 2426, 5343, 5650, 5829
Hpy99I CGWCG 2 cut(s) 1266, 1799
HpyCH4IV ACGT 9 cut(s) 2027, 2350, 2472, 2752, 3059, 4089, 4663, 5837, 6057
HpySE526I ACGT 9 cut(s) 2027, 2350, 2472, 2752, 3059, 4089, 4663, 5837, 6057
Hsp92I GRCGYC 3 cut(s) 2382, 3059, 5837
HspAI GCGC 5 cut(s) 266, 1616, 2576, 3852, 4684
KpnI GGTACC 2 cut(s) 3930, 5769
KroI GCCGGC 2 cut(s) 2095, 5342
KroNI GCCGGC 2 cut(s) 2097, 5344
Ksp22I TGATCA 2 cut(s) 1477, 5800
KspAI GTTAAC 2 cut(s) 472, 2524
MaeII ACGT 9 cut(s) 2027, 2350, 2472, 2752, 3059, 4089, 4663, 5837, 6057
MbiI CCGCTC 2 cut(s) 1591, 5665
MfeI CAATTG 3 cut(s) 53, 854, 5406
MflI RGATCY 5 cut(s) 1435, 1790, 4796, 5387, 6198
MhlI GDGCHC 5 cut(s) 875, 3872, 4106, 4562, 5177
MlsI TGGCCA 3 cut(s) 3004, 3755, 4844
MluNI TGGCCA 3 cut(s) 3004, 3755, 4844
MlyI GAGTC 4 cut(s) 2174, 2939, 3147, 4133
MmeI TCCRAC 4 cut(s) 171, 1630, 3337, 4867
Mox20I TGGCCA 3 cut(s) 3004, 3755, 4844
Mph1103I ATGCAT 4 cut(s) 1902, 4719, 5707, 6135
MroNI GCCGGC 2 cut(s) 2095, 5342
MroXI GAANNNNTTC 6 cut(s) 762, 1561, 1959, 4633, 5917, 6318
MscI TGGCCA 3 cut(s) 3004, 3755, 4844
MslI CAYNNNNRTG 9 cut(s) 1534, 2328, 3531, 3703, 4722, 4830, 4880, 5180, 5472
Msp20I TGGCCA 3 cut(s) 3004, 3755, 4844
MspA1I CMGCKG 7 cut(s) 568, 2150, 2921, 4292, 4760, 6004, 6145
MspI CCGG 7 cut(s) 542, 2096, 2255, 2426, 5343, 5650, 5829
MspR9I CCNGG 9 cut(s) 524, 2256, 3024, 3249, 3812, 4398, 5385, 5650, 6206
MunI CAATTG 3 cut(s) 53, 854, 5406
Mva1269I GAATGC 4 cut(s) 133, 1639, 2074, 3108
MvaI CCWGG 7 cut(s) 524, 3024, 3249, 3812, 4398, 5385, 6206
MvnI CGCG 1 cut(s) 3653
NaeI GCCGGC 2 cut(s) 2097, 5344
NciI CCSGG 2 cut(s) 2256, 5650
NdeI CATATG 1 cut(s) 5701
NgoMIV GCCGGC 2 cut(s) 2095, 5342
NheI GCTAGC 2 cut(s) 592, 2905
NmeAIII GCCGAG 1 cut(s) 1007
NsiI ATGCAT 4 cut(s) 1902, 4719, 5707, 6135
NspI RCATGY 9 cut(s) 1539, 2204, 2219, 3163, 3384, 3401, 3536, 4721, 5383
NspV TTCGAA 1 cut(s) 2566
OliI CACNNNNGTG 2 cut(s) 4880, 5180
PaeI GCATGC 3 cut(s) 2204, 3163, 3536
PagI TCATGA 1 cut(s) 1045
PceI AGGCCT 2 cut(s) 2237, 4705
PciI ACATGT 1 cut(s) 1535
PcsI WCGNNNNNNNCGW 2 cut(s) 4095, 4334
PctI GAATGC 4 cut(s) 133, 1639, 2074, 3108
PdiI GCCGGC 2 cut(s) 2097, 5344
PdmI GAANNNNTTC 6 cut(s) 762, 1561, 1959, 4633, 5917, 6318
PflMI CCANNNNNTGG 4 cut(s) 310, 856, 3011, 5909
PleI GAGTC 4 cut(s) 2174, 2939, 3146, 4133
PpsI GAGTC 4 cut(s) 2174, 2939, 3146, 4133
Ppu21I YACGTR 1 cut(s) 4664
PpuMI RGGWCCY 3 cut(s) 5030, 5423, 5642
PscI ACATGT 1 cut(s) 1535
PshAI GACNNNNGTC 1 cut(s) 6161
PshBI ATTAAT 1 cut(s) 4044
Psp124BI GAGCTC 2 cut(s) 3872, 4562
Psp1406I AACGTT 1 cut(s) 4089
Psp5II RGGWCCY 3 cut(s) 5030, 5423, 5642
Psp6I CCWGG 7 cut(s) 522, 3022, 3247, 3810, 4396, 5383, 6204
PspFI CCCAGC 2 cut(s) 6254, 6264
PspGI CCWGG 7 cut(s) 522, 3022, 3247, 3810, 4396, 5383, 6204
PspPPI RGGWCCY 3 cut(s) 5030, 5423, 5642
PstI CTGCAG 1 cut(s) 2663
PstNI CAGNNNCTG 6 cut(s) 571, 1408, 2153, 2948, 3329, 6361
PsuI RGATCY 5 cut(s) 1435, 1790, 4796, 5387, 6198
PvuII CAGCTG 7 cut(s) 568, 2150, 2921, 4292, 4760, 6004, 6145
RsaI GTAC 8 cut(s) 6, 380, 907, 2846, 3928, 4198, 5687, 5767
RsaNI GTAC 8 cut(s) 5, 379, 906, 2845, 3927, 4197, 5686, 5766
RseI CAYNNNNRTG 9 cut(s) 1534, 2328, 3531, 3703, 4722, 4830, 4880, 5180, 5472
SacI GAGCTC 2 cut(s) 3872, 4562
ScaI AGTACT 1 cut(s) 5687
SchI GAGTC 4 cut(s) 2174, 2939, 3147, 4133
ScrFI CCNGG 9 cut(s) 524, 2256, 3024, 3249, 3812, 4398, 5385, 5650, 6206
SduI GDGCHC 5 cut(s) 875, 3872, 4106, 4562, 5177
SfcI CTRYAG 5 cut(s) 531, 2659, 2825, 3094, 5727
SfuI TTCGAA 1 cut(s) 2566
SinI GGWCC 7 cut(s) 2776, 3080, 3209, 3251, 5030, 5423, 5642
SmiMI CAYNNNNRTG 9 cut(s) 1534, 2328, 3531, 3703, 4722, 4830, 4880, 5180, 5472
SmlI CTYRAG 4 cut(s) 144, 440, 1892, 1934
SmoI CTYRAG 4 cut(s) 144, 440, 1892, 1934
SphI GCATGC 3 cut(s) 2204, 3163, 3536
SseBI AGGCCT 2 cut(s) 2237, 4705
SspI AATATT 2 cut(s) 2689, 4357
SstI GAGCTC 2 cut(s) 3872, 4562
StuI AGGCCT 2 cut(s) 2237, 4705
StyD4I CCNGG 9 cut(s) 522, 2254, 3022, 3247, 3810, 4396, 5383, 5648, 6204
StyI CCWWGG 3 cut(s) 1851, 3075, 5717
TaiI ACGT 9 cut(s) 2030, 2353, 2475, 2755, 3062, 4092, 4666, 5840, 6060
TatI WGTACW 4 cut(s) 378, 905, 2844, 5685
TauI GCSGC 1 cut(s) 5944
TspGWI ACGGA 1 cut(s) 1783
Van91I CCANNNNNTGG 4 cut(s) 310, 856, 3011, 5909
VpaK11BI GGWCC 7 cut(s) 2776, 3080, 3209, 3251, 5030, 5423, 5642
VspI ATTAAT 1 cut(s) 4044
XagI CCTNNNNNAGG 1 cut(s) 2231
XbaI TCTAGA 3 cut(s) 502, 3631, 4427
XceI RCATGY 9 cut(s) 1539, 2204, 2219, 3163, 3384, 3401, 3536, 4721, 5383
XcmI CCANNNNNNNNNTGG 2 cut(s) 3012, 5474
XmiI GTMKAC 2 cut(s) 3489, 4810
XmnI GAANNNNTTC 6 cut(s) 762, 1561, 1959, 4633, 5917, 6318
ZraI GACGTC 2 cut(s) 3060, 5838
ZrmI AGTACT 1 cut(s) 5687
Zsp2I ATGCAT 4 cut(s) 1902, 4719, 5707, 6135
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.