MD12G1041400.v1.1

Protein virilizer homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0010141
Physical Location & Seq
Reverse (-)
4574188 .. 4587063
12876 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1041400.v1.1.491

Sequence Viewer

Length: 4143 bp
ATGGGTCGCCCCGAGCCTTGTGTGTTGTTCGCCCAGACCTTCGTCCACCCCCATCTCGATGAATACGTCGACGAGGTATTGTTTGCTGAGCCCATTGTCATCACTGCTTGCGAGTTCCTCGAACAGAACGTGTCGTTTGCTTCTCAGGCAGTATCACTTCTCGGGGCTACTTCACCTCCTTCATTTGCTTTGGAGGTATTTGTTCAATGTGAAGGGGAGACAAGGTTCAGGCGCCTCTGTCAGCCTTTCCTATATTCTCCTTCTTCTTCAAATGTGCTAGAAGTAGAGGCTGTTGTAACTAATCATCTCGTTGTAAGGGGCAGCTACCGCAGTCTGACCTTAGTCATATATGGAAACACGGCAGAAGATCTAGGGCAATTTAACATTGAAATTGATGATAGCTCGATAACTAATCTTGTTAGCTCTTCTGAGGGAAAGCTTGAAGACCTCCCTCTTGCTTTACATTCAACTAATCTGACAACTGGGGAATCCATATCAGCTCTTAATACTCTATCTCTACCTGTTGCGGCATCAGATATATCTGTTGAAGCTAAGCATTTATTACAACTGATGTTGAAGGTTTTTGAGTTGCCCAATCTAGGTGATGCATTGCATAAAGTTGTCGCTGCTGTAGTAACAGCTGCCACTTCTTATGTCACCTCCTCTTGGGGAAGATCCAATGAGTGTGAAGAGTTGCGCAATGTTCTTAGTGAGGCCAGAACAAAGGTTATGGAGCTCTATAAAGTCGTTAAACACGAATCAGGGAATGGCTTAGCTGAATCACATGAAGACAACGGGCTTTTTGAGTTTGAGGCTGAATTAGCAAATTCTAAACAGTTGGTGGATGTGTTAAGTCAGTACTTTCAGTTTAGTAGGGAGTTCTTGAGTGTTGTGCATCACCAACTTCCGCAGAATACAAATGTCATGTTGGGGTTGAGTGTGGCTTTTCTTTTGTGCTCTGGAAGAGAGAGTTGCTTTCATTTTGTTAGTGGTGGGGGAATGGAGCAGCTTGTACATGCTTTTTGTTGTGACAAGCAGAATTCAACTGCCACTACATTACTACTACTTGGAGTTGTTGAGAAGGCTACTCAGCATTCTTTTGGGTGTGAAGGATTTTTAGGTTGGTGGCCACGTGAAGATGAAAACATCCCATCCGGTGTCAGTGATGGTTATAGTAGATTGTTAAATCTGTTATTGCAAAAACAGCGTCATGATATTGCTTCTTGTGCAACTTACGTCCTTCATCGGTTAAGATTTTATGAGGTTGCTTCAAGATTCGAGTGCGCAGCTTTATCGGTTTTGGGTGGTCTATCTACTGTTGGTAGAGTCACAAGTGGAACTCTGGACATGCTTATCTGTGCAAAGTCACAACTTAAAAAGCTGTTGAAATTGATAAATTCACGTGGTCCAATTGAAGATCCTTCTCCAGTTGCCCAAGCAACCAAATCCTTGATTCTTGGTCAGATTGAAGGTTTGTTGTCAATCAAAGCATCCAATAACTTGATTGCTTCTTCAAATTGTTGTTTCTCAAATTCGGATATCGACATGCATTTGCTGGCTCTTCTCAAGGAGAGGGGATTTCTACCTCTATCAGTTGCAATATTGTCGTCATCCATATTGCGTTCAGAAGTCGGATGTGTGAGGGACATATTTGTGGATGTTGTGTCATCAATTGAGGCCATAATTCTCTCCCTTCTTTTTTGTCGCTCAGGCTTAATTTTTCTTCTGCACCACCCTGAACTTTCTGCAACAATAATAGGTGCACTAAGGGGTGCTGATGATGTGAATAAGGATGCGTGTCTTCCTCTTCGATATGCATTTATTTCATTATCCAAAGGATTCTTTTGTGCTCCACAGGATGTTGGAATGATTGTTGGGGTCCATTTGAGGGTGGTTAATGCTGTAGACCGTTTGCTTACTGCAGCACCTAACTCTGAAGAATTTTTATGGGTGTTGTGGGAACTGTGTGGTCTTGCAAGGTCTGACTGTGGACGTCAAGCATTATTGGCTCTGGGGTATTTTCCGGAGGCTGTTAAAATTTTGATTGAAGCTTTACATTCTGCCAAGGAAACAGAGCCGCTTGCTAAAAATAATGGAGCCTCGCCGTTAAATATTGCCATATTTCACTCAGCTGCTGAGATTTTTGAAGTGATTGTTTCTGATTCCACTGCATCTTCTCTTGGATCTTGGATTGGACATGTTATGGAGCTCCATAGGGCTTTACATTCCTCTTCTCCTGGGTCAAATAGGAAAGATGCCCCCACACGGCTGTTGGAATGGATAGATGCTGGTGTAGTATATCATAAAAATGGGGCAACAAGTCTTATACGGTATGTTGCTATGTTGGCTTCTGGAGGAGATGCTCACTTGACTTCAAACATACCGCTGGGGGCAGATTTGGCAGATGTAGAGAATGCAATTGGAGATACATCTAGTGGTTCTGATGTGAATGTTATGGAAAATCTTGGAAAGTTTATATCTGACAAGACTTTTGATGGTGTTAATCTTCGTGACTCTTCTGTAGTACAGTTGACAACAGCATTTCGCATTTTAGCGTTCATTTCAGAGAACTCAACTGTTGCTGCTACTTTGTATGATGAAGGTGTTATACCAATAATCTATGCAGTTCTGGTCAATTGTAGGTCTATGCTTGAGAGGTCCTCAAACAGTTATGACTACCTTGTTGATGAGGAGTGCAATACTACATCAGATTTATTGTCGGAACGTAATCATGAGCAGAGTTTAGTTGATCTTCTTATTCCTACAATTGTGTTGTTGATCAATTTGTTGCAGAAATTGCAGGAAGTACAAGAGCAGCACAGAAATACCAAGTTATTGAATGCCCTTCTACGATTGCATAGAGAAGTAAGTCCAAAATTAGCCGCATGTGCAGCAGACTTATCCTCTCCATATCCTGTTTCTGCACTTGGTTTTGGAGCTATCTGTCATCTTCTGGTGTCGGCACTTGCTTGTTGGCCGGTCTATGGTTGGACTCCTGGCGTTTTTCACTCCCTCCTTGCTAATGTTCAAGTTCCTTCTTTGCTGGCCTTGGGTCCAAAGGAAACATGTAGTTTGCTTTGTCTTCTGAATGATTTATTTCCTGATGAAGGGGTCTGGCTCTGGAAAAATGGGATGCCCTTGTTGAGTGCCCTTAGAAAGTTGTCTGTTGGGACTGTTTTGGGTCCTCAGAAGGAGAGACAGGTCAATTGGTATTTGCATCATGTGCACCTGGAGAAGATGCTCAGCCAATTGACGCCACATCTTGACAAAGTTGCCCAGATTATTCAACATTATGCTATCTCCGCACTGGTGGTCATTCAAGACATGCTTCGAGTCTTTATTATTCGTATTGCTTGCCTAAGAGCTCAAAGTTTTCCCATACTTTTACGACCCATATTCTCATGGATTCGTGATCATGCGTATGATACATCTTCGCCATCAGATACAGATGCTTATAAGGTTTATAGATATATTGATTTCATTGCTAGCTTATTGGAGCATCCACGTGCCAAGTCACTATTATTGAAGGAGGGTGCATTTAAGTTGCTAATCAGAGTGTTGGATAGTTGTTTAGTTGCCACTGAGACAGAAGGAATAGAGAATCTTGATGGTAGAAGTTCAGCTACATTTGGATTCGGTTTGCTCAATTGGTGCCTCCCTGTATTCAAGTCCTTCTCATTGATTTTCATTCCTCAAGCATCTCCACACCATGCTGGGGGAAATGATTTGCACGAATTTGAAAAGTTGAGTACTGAAGATTGCAAAATCTTTTTAAAGTATTTCCTCAGGTTTTGTCAGGTTCTACCAGTTGGAAAAGAATTACTTGCCTGTCTTACTGCTTTTAAAGAACTGGGTGCTTGCAGTGAAGGTCAAAAAGCTTTAGCAGCCACTTTATATCCTGCCCTCTCTGTTGAGGACCATGAATGGAGAAAGAGTCCCCCATTGTTAAGTTGCTGTAAAAACTTGTTAAGATCAGTTTACTCTAAAGATGGGTTGTCAAGTTATGGAATTGAGGCCGTCAATGCATTGTCTGTGGGATCTTTCCGCTTCTGCCTTGATGGGGAAAGGGTTTGTGCTACAGGTTGTACCTTTCTGTCCTTGAGTTCTGAAGTAATTTGGGGCGTCTCTATGCACATGTATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
VIR_N

Protein Analysis

1381

Amino Acids

151.25

Weight (kDa)

5.56

Isoelectric Point (pI)

42.89

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
VIR_N PF15912 8 - 121 4.8e-08 Virilizer, N-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0010141)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G05680 AT3G05680
fragaria_vesca FvH4_6g20410 FvH4_6g20410 FvH4_6g20410
malus_domestica MD12G1041200.v1.1 MD12G1041400.v1.1 MD14G1040500.v1.1
prunus_persica Prupe.7G093800_v2.0.a1
pyrus_communis pycom12g03590 pycom14g03440
rosa_chinensis RchiOBHm_Chr3g0476141
rosa_laevigata RLG00000023788
rosa_multiflora Rmu_sc0000114.1_g000025
rosa_roxburghii Rroxscaffold_6G00405450
rosa_rugosa Rorug03G0153000
rosa_samantha Rh3AG204100 Rh3BG234300 Rh3CG229700 Rh3DG229700
rosa_wichuraiana Rw3G018550

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 3456
AatII GACGTC 1 cut(s) 1996
Acc16I TGCGCA 2 cut(s) 698, 1285
AccB1I GGYRCC 2 cut(s) 231, 3651
AccI GTMKAC 2 cut(s) 69, 1905
AccIII TCCGGA 1 cut(s) 2023
AciI CCGC 8 cut(s) 328, 527, 908, 2078, 2384, 2883, 3303, 4045
AclWI GGATC 4 cut(s) 669, 1412, 2191, 4045
AcoI YGGCCR 2 cut(s) 1127, 2975
AcsI RAATTY 7 cut(s) 826, 1039, 1396, 1531, 1940, 2037, 3734
AcuI CTGAAG 3 cut(s) 1956, 3774, 4128
AcvI CACGTG 3 cut(s) 1133, 1403, 3506
AcyI GRCGYC 4 cut(s) 232, 1993, 3254, 4122
AfaI GTAC 6 cut(s) 860, 1014, 2526, 2808, 3751, 4087
AfiI CCNNNNNNNGG 8 cut(s) 599, 666, 1888, 2265, 2984, 3107, 3715, 4060
AflIII ACRYGT 4 cut(s) 129, 2197, 3065, 4134
AjnI CCWGG 3 cut(s) 2236, 2995, 3228
AloI GAACNNNNNNTCC 2 cut(s) 209, 241
Alw21I GWGCWC 7 cut(s) 738, 959, 1765, 1852, 2211, 3228, 3367
Alw26I GTCTC 4 cut(s) 212, 3190, 3578, 4129
Alw44I GTGCAC 2 cut(s) 1761, 3224
AlwI GGATC 4 cut(s) 669, 1412, 2191, 4045
AlwNI CAGNNNCTG 1 cut(s) 2135
Ama87I CYCGRG 2 cut(s) 11, 161
Aor13HI TCCGGA 1 cut(s) 2023
AoxI GGCC 6 cut(s) 714, 1127, 1677, 2975, 3045, 4014
ApaLI GTGCAC 2 cut(s) 1761, 3224
ApoI RAATTY 7 cut(s) 826, 1039, 1396, 1531, 1940, 2037, 3734
ArsI GACNNNNNNTTYG 4 cut(s) 1192, 1224, 2474, 2506
AspLEI GCGC 3 cut(s) 234, 699, 1286
AspS9I GGNCC 6 cut(s) 1406, 1879, 2658, 3053, 3182, 3916
AsuHPI GGTGA 4 cut(s) 165, 614, 649, 890
AsuNHI GCTAGC 1 cut(s) 3485
AvaI CYCGRG 2 cut(s) 11, 161
AvaII GGWCC 6 cut(s) 1406, 1879, 2658, 3053, 3182, 3916
AxyI CCTNAGG 1 cut(s) 3785
BaeGI GKGCMC 3 cut(s) 1765, 3151, 3228
BalI TGGCCA 1 cut(s) 1129
BanI GGYRCC 2 cut(s) 231, 3651
BanII GRGCYC 4 cut(s) 93, 738, 2211, 3367
BarI GAAGNNNNNNTAC 4 cut(s) 2592, 2624, 3607, 3639
BbrPI CACGTG 3 cut(s) 1133, 1403, 3506
BbsI GAAGAC 4 cut(s) 450, 795, 1793, 3074
Bbv12I GWGCWC 7 cut(s) 738, 959, 1765, 1852, 2211, 3228, 3367
BccI CCATC 8 cut(s) 60, 1159, 1160, 2489, 3445, 3602, 3983, 4052
BceAI ACGGC 4 cut(s) 375, 2089, 2282, 4001
BcgI CGANNNNNNTGC 4 cut(s) 1275, 1309, 1587, 1621
BciT130I CCWGG 3 cut(s) 2238, 2997, 3230
BclI TGATCA 2 cut(s) 2778, 3412
BcoDI GTCTC 4 cut(s) 212, 3190, 3578, 4129
BfaI CTAG 5 cut(s) 278, 371, 599, 2433, 3486
BfmI CTRYAG 5 cut(s) 630, 1902, 1920, 2520, 4077
BfoI RGCGCY 1 cut(s) 235
BglII AGATCT 1 cut(s) 367
BlpI GCTNAGC 4 cut(s) 87, 552, 772, 3242
BmcAI AGTACT 2 cut(s) 860, 3751
Bme1390I CCNGG 3 cut(s) 2238, 2997, 3230
Bme18I GGWCC 6 cut(s) 1406, 1879, 2658, 3053, 3182, 3916
BmeT110I CYCGRG 2 cut(s) 11, 161
BmgT120I GGNCC 6 cut(s) 1406, 1879, 2658, 3053, 3182, 3916
BmiI GGNNCC 6 cut(s) 233, 1880, 2098, 3054, 3183, 3653
BmrFI CCNGG 3 cut(s) 2238, 2997, 3230
BmrI ACTGGG 2 cut(s) 492, 3860
BmtI GCTAGC 1 cut(s) 3489
BmuI ACTGGG 2 cut(s) 492, 3860
BoxI GACNNNNGTC 2 cut(s) 41, 341
BpiI GAAGAC 4 cut(s) 450, 795, 1793, 3074
BplI GAGNNNNNCTC 2 cut(s) 2645, 2677
BpmI CTGGAG 3 cut(s) 1410, 2373, 3251
Bpu10I CCTNAGC 1 cut(s) 1708
Bpu1102I GCTNAGC 4 cut(s) 87, 552, 772, 3242
BpuEI CTTGAG 5 cut(s) 904, 1550, 2672, 3678, 4120
BsaAI YACGTR 3 cut(s) 1133, 1403, 3506
BsaHI GRCGYC 4 cut(s) 232, 1993, 3254, 4122
BsaJI CCNNGG 3 cut(s) 2064, 2237, 3048
BsaWI WCCGGW 2 cut(s) 1154, 2023
BsaXI ACNNNNNCTCC 6 cut(s) 160, 190, 209, 239, 3919, 3949
Bsc4I CCNNNNNNNGG 8 cut(s) 599, 666, 1888, 2265, 2984, 3107, 3715, 4060
Bse118I RCCGGY 1 cut(s) 2977
Bse1I ACTGG 5 cut(s) 487, 1427, 3312, 3806, 3855
Bse21I CCTNAGG 1 cut(s) 3785
Bse3DI GCAATG 3 cut(s) 608, 706, 3480
BseAI TCCGGA 1 cut(s) 2023
BseBI CCWGG 3 cut(s) 2238, 2997, 3230
BseDI CCNNGG 3 cut(s) 2064, 2237, 3048
BseLI CCNNNNNNNGG 8 cut(s) 599, 666, 1888, 2265, 2984, 3107, 3715, 4060
BseMI GCAATG 3 cut(s) 608, 706, 3480
BseNI ACTGG 5 cut(s) 487, 1427, 3312, 3806, 3855
BseRI GAGGAG 3 cut(s) 652, 2370, 2705
BseSI GKGCMC 3 cut(s) 1765, 3151, 3228
BseYI CCCAGC 2 cut(s) 2386, 3713
BsgI GTGCAG 3 cut(s) 1712, 2907, 2910
BshFI GGCC 6 cut(s) 716, 1129, 1679, 2977, 3047, 4016
BshNI GGYRCC 2 cut(s) 231, 3651
BsiHKAI GWGCWC 7 cut(s) 738, 959, 1765, 1852, 2211, 3228, 3367
BsiHKCI CYCGRG 2 cut(s) 11, 161
BsiSI CCGG 3 cut(s) 1155, 2024, 2978
BslFI GGGAC 3 cut(s) 1658, 3184, 3921
BslI CCNNNNNNNGG 8 cut(s) 599, 666, 1888, 2265, 2984, 3107, 3715, 4060
BsmAI GTCTC 4 cut(s) 212, 3190, 3578, 4129
BsmBI CGTCTC 1 cut(s) 4129
BsmFI GGGAC 3 cut(s) 1658, 3184, 3921
BsmI GAATGC 3 cut(s) 1093, 2419, 2845
BsnI GGCC 6 cut(s) 716, 1129, 1679, 2977, 3047, 4016
BsoBI CYCGRG 2 cut(s) 11, 161
Bsp1286I GDGCHC 9 cut(s) 93, 738, 959, 1765, 1852, 2211, 3151, 3228, 3367
Bsp13I TCCGGA 1 cut(s) 2023
Bsp1407I TGTACA 1 cut(s) 1012
Bsp143I GATC 9 cut(s) 367, 674, 1417, 2183, 2749, 2778, 3412, 3971, 4037
Bsp1720I GCTNAGC 4 cut(s) 87, 552, 772, 3242
BspACI CCGC 8 cut(s) 328, 527, 908, 2078, 2384, 2883, 3303, 4045
BspANI GGCC 6 cut(s) 716, 1129, 1679, 2977, 3047, 4016
BspEI TCCGGA 1 cut(s) 2023
BspHI TCATGA 2 cut(s) 1210, 2731
BspLI GGNNCC 6 cut(s) 233, 1880, 2098, 3054, 3183, 3653
BspMAI CTGCAG 1 cut(s) 1924
BspOI GCTAGC 1 cut(s) 3489
BspPI GGATC 4 cut(s) 669, 1412, 2191, 4045
BspQI GCTCTTC 2 cut(s) 430, 1566
BspT107I GGYRCC 2 cut(s) 231, 3651
BsrDI GCAATG 3 cut(s) 608, 706, 3480
BsrFI RCCGGY 1 cut(s) 2977
BsrGI TGTACA 1 cut(s) 1012
BsrI ACTGG 5 cut(s) 487, 1427, 3312, 3806, 3855
BssAI RCCGGY 1 cut(s) 2977
BssECI CCNNGG 3 cut(s) 2064, 2237, 3048
BssMI GATC 9 cut(s) 367, 674, 1417, 2183, 2749, 2778, 3412, 3971, 4037
BssNI GRCGYC 4 cut(s) 232, 1993, 3254, 4122
BssT1I CCWWGG 2 cut(s) 2064, 3048
Bst2UI CCWGG 3 cut(s) 2238, 2997, 3230
Bst6I CTCTTC 7 cut(s) 430, 684, 958, 1566, 1812, 2236, 2521
BstACI GRCGYC 4 cut(s) 232, 1993, 3254, 4122
BstAPI GCANNNNNTGC 2 cut(s) 2797, 3223
BstAUI TGTACA 1 cut(s) 1012
BstBAI YACGTR 3 cut(s) 1133, 1403, 3506
BstC8I GCNNGC 7 cut(s) 109, 1557, 2082, 3045, 3355, 3487, 3859
BstENI CCTNNNNNAGG 1 cut(s) 3105
BstH2I RGCGCY 1 cut(s) 235
BstHHI GCGC 3 cut(s) 234, 699, 1286
BstKTI GATC 9 cut(s) 370, 677, 1420, 2186, 2752, 2781, 3415, 3974, 4040
BstMAI GTCTC 4 cut(s) 212, 3190, 3578, 4129
BstMBI GATC 9 cut(s) 367, 674, 1417, 2183, 2749, 2778, 3412, 3971, 4037
BstNI CCWGG 3 cut(s) 2238, 2997, 3230
BstNSI RCATGY 8 cut(s) 1019, 1351, 1549, 2201, 2889, 3069, 3328, 4138
BstPAI GACNNNNGTC 2 cut(s) 41, 341
BstSCI CCNGG 3 cut(s) 2236, 2995, 3228
BstSFI CTRYAG 5 cut(s) 630, 1902, 1920, 2520, 4077
BstSLI GKGCMC 3 cut(s) 1765, 3151, 3228
BstV2I GAAGAC 4 cut(s) 450, 795, 1793, 3074
BstX2I RGATCY 5 cut(s) 367, 674, 1417, 2183, 4037
BstYI RGATCY 5 cut(s) 367, 674, 1417, 2183, 4037
Bsu36I CCTNAGG 1 cut(s) 3785
BsuRI GGCC 6 cut(s) 716, 1129, 1679, 2977, 3047, 4016
BtsI GCAGTG 3 cut(s) 102, 2166, 3868
BtsIMutI CAGTG 6 cut(s) 102, 1168, 2166, 3305, 3579, 3868
Cac8I GCNNGC 7 cut(s) 109, 1557, 2082, 3045, 3355, 3487, 3859
CaiI CAGNNNCTG 1 cut(s) 2135
CciI TCATGA 2 cut(s) 1210, 2731
CfoI GCGC 3 cut(s) 234, 699, 1286
Cfr10I RCCGGY 1 cut(s) 2977
Cfr13I GGNCC 6 cut(s) 1406, 1879, 2658, 3053, 3182, 3916
CseI GACGC 3 cut(s) 1196, 3262, 4111
Csp6I GTAC 6 cut(s) 859, 1013, 2525, 2807, 3750, 4086
CviQI GTAC 6 cut(s) 859, 1013, 2525, 2807, 3750, 4086
DinI GGCGCC 1 cut(s) 233
DpnI GATC 9 cut(s) 369, 676, 1419, 2185, 2751, 2780, 3414, 3973, 4039
DpnII GATC 9 cut(s) 367, 674, 1417, 2183, 2749, 2778, 3412, 3971, 4037
DraI TTTAAA 2 cut(s) 3774, 3844
EaeI YGGCCR 2 cut(s) 1127, 2975
Eam1104I CTCTTC 7 cut(s) 430, 684, 958, 1566, 1812, 2236, 2521
EarI CTCTTC 7 cut(s) 430, 684, 958, 1566, 1812, 2236, 2521
Ecl136II GAGCTC 3 cut(s) 736, 2209, 3365
Eco130I CCWWGG 2 cut(s) 2064, 3048
Eco24I GRGCYC 4 cut(s) 93, 738, 2211, 3367
Eco32I GATATC 1 cut(s) 1540
Eco47I GGWCC 6 cut(s) 1406, 1879, 2658, 3053, 3182, 3916
Eco53kI GAGCTC 3 cut(s) 736, 2209, 3365
Eco57I CTGAAG 3 cut(s) 1956, 3774, 4128
Eco72I CACGTG 3 cut(s) 1133, 1403, 3506
Eco81I CCTNAGG 1 cut(s) 3785
Eco88I CYCGRG 2 cut(s) 11, 161
EcoICRI GAGCTC 3 cut(s) 736, 2209, 3365
EcoNI CCTNNNNNAGG 1 cut(s) 3105
EcoO109I RGGNCCY 2 cut(s) 2658, 3182
EcoRI GAATTC 1 cut(s) 1039
EcoRII CCWGG 3 cut(s) 2236, 2995, 3228
EcoRV GATATC 1 cut(s) 1540
EcoT14I CCWWGG 2 cut(s) 2064, 3048
EcoT22I ATGCAT 4 cut(s) 610, 1551, 1819, 4027
EcoT38I GRGCYC 4 cut(s) 93, 738, 2211, 3367
EgeI GGCGCC 1 cut(s) 233
EheI GGCGCC 1 cut(s) 233
ErhI CCWWGG 2 cut(s) 2064, 3048
Esp3I CGTCTC 1 cut(s) 4129
FalI AAGNNNNNCTT 4 cut(s) 3312, 3344, 3807, 3839
FaqI GGGAC 3 cut(s) 1658, 3184, 3921
FbaI TGATCA 2 cut(s) 2778, 3412
FblI GTMKAC 2 cut(s) 69, 1905
FriOI GRGCYC 4 cut(s) 93, 738, 2211, 3367
FspBI CTAG 5 cut(s) 278, 371, 599, 2433, 3486
FspI TGCGCA 2 cut(s) 698, 1285
GlaI GCGC 3 cut(s) 233, 698, 1285
GsaI CCCAGC 2 cut(s) 2390, 3717
GsuI CTGGAG 3 cut(s) 1410, 2373, 3251
HaeII RGCGCY 1 cut(s) 235
HaeIII GGCC 6 cut(s) 716, 1129, 1679, 2977, 3047, 4016
HapII CCGG 3 cut(s) 1155, 2024, 2978
HgaI GACGC 3 cut(s) 1196, 3262, 4111
HhaI GCGC 3 cut(s) 234, 699, 1286
Hin1I GRCGYC 4 cut(s) 232, 1993, 3254, 4122
Hin6I GCGC 3 cut(s) 232, 697, 1284
HinP1I GCGC 3 cut(s) 232, 697, 1284
HincII GTYRAC 2 cut(s) 70, 2532
HindII GTYRAC 2 cut(s) 70, 2532
HindIII AAGCTT 3 cut(s) 437, 2049, 3876
HpaII CCGG 3 cut(s) 1155, 2024, 2978
HphI GGTGA 4 cut(s) 165, 614, 649, 890
Hpy166II GTNNAC 8 cut(s) 46, 70, 1763, 1906, 1991, 2532, 3226, 3979
Hpy8I GTNNAC 8 cut(s) 46, 70, 1763, 1906, 1991, 2532, 3226, 3979
Hpy99I CGWCG 2 cut(s) 71, 74
HpyCH4IV ACGT 8 cut(s) 66, 129, 1132, 1236, 1402, 1993, 2725, 3505
HpySE526I ACGT 8 cut(s) 66, 129, 1132, 1236, 1402, 1993, 2725, 3505
Hsp92I GRCGYC 4 cut(s) 232, 1993, 3254, 4122
HspAI GCGC 3 cut(s) 232, 697, 1284
KasI GGCGCC 1 cut(s) 231
Kpn2I TCCGGA 1 cut(s) 2023
Ksp22I TGATCA 2 cut(s) 2778, 3412
Kzo9I GATC 9 cut(s) 367, 674, 1417, 2183, 2749, 2778, 3412, 3971, 4037
LguI GCTCTTC 2 cut(s) 430, 1566
LmnI GCTCC 8 cut(s) 733, 1003, 1855, 2096, 2206, 2214, 2936, 3496
MaeI CTAG 5 cut(s) 278, 371, 599, 2433, 3486
MaeII ACGT 8 cut(s) 66, 129, 1132, 1236, 1402, 1993, 2725, 3505
MaeIII GTNAC 8 cut(s) 295, 634, 655, 1028, 1327, 1365, 2510, 3513
MalI GATC 9 cut(s) 369, 676, 1419, 2185, 2751, 2780, 3414, 3973, 4039
MboI GATC 9 cut(s) 367, 674, 1417, 2183, 2749, 2778, 3412, 3971, 4037
MfeI CAATTG 8 cut(s) 1410, 1671, 2418, 2635, 2766, 3205, 3248, 3646
MflI RGATCY 5 cut(s) 367, 674, 1417, 2183, 4037
MhlI GDGCHC 9 cut(s) 93, 738, 959, 1765, 1852, 2211, 3151, 3228, 3367
MlsI TGGCCA 1 cut(s) 1129
MluNI TGGCCA 1 cut(s) 1129
Mly113I GGCGCC 1 cut(s) 232
MlyI GAGTC 5 cut(s) 1335, 2507, 2986, 3342, 3943
MmeI TCCRAC 7 cut(s) 1612, 1843, 2253, 2700, 2969, 3540, 3790
Mox20I TGGCCA 1 cut(s) 1129
Mph1103I ATGCAT 4 cut(s) 610, 1551, 1819, 4027
MroI TCCGGA 1 cut(s) 2023
MscI TGGCCA 1 cut(s) 1129
MslI CAYNNNNRTG 5 cut(s) 57, 1652, 2307, 3420, 3504
Msp20I TGGCCA 1 cut(s) 1129
MspA1I CMGCKG 3 cut(s) 641, 2132, 2386
MspI CCGG 3 cut(s) 1155, 2024, 2978
MspR9I CCNGG 3 cut(s) 2238, 2997, 3230
MunI CAATTG 8 cut(s) 1410, 1671, 2418, 2635, 2766, 3205, 3248, 3646
Mva1269I GAATGC 3 cut(s) 1093, 2419, 2845
MvaI CCWGG 3 cut(s) 2238, 2997, 3230
NarI GGCGCC 1 cut(s) 232
NdeII GATC 9 cut(s) 367, 674, 1417, 2183, 2749, 2778, 3412, 3971, 4037
NheI GCTAGC 1 cut(s) 3485
NlaIV GGNNCC 6 cut(s) 233, 1880, 2098, 3054, 3183, 3653
NmuCI GTSAC 6 cut(s) 655, 1028, 1327, 1365, 2510, 3513
NsbI TGCGCA 2 cut(s) 698, 1285
NsiI ATGCAT 4 cut(s) 610, 1551, 1819, 4027
NspI RCATGY 8 cut(s) 1019, 1351, 1549, 2201, 2889, 3069, 3328, 4138
PagI TCATGA 2 cut(s) 1210, 2731
PciI ACATGT 3 cut(s) 2197, 3065, 4134
PciSI GCTCTTC 2 cut(s) 430, 1566
PcsI WCGNNNNNNNCGW 3 cut(s) 63, 126, 753
PctI GAATGC 3 cut(s) 1093, 2419, 2845
PleI GAGTC 5 cut(s) 1334, 2507, 2986, 3341, 3942
PluTI GGCGCC 1 cut(s) 235
PmaCI CACGTG 3 cut(s) 1133, 1403, 3506
PmlI CACGTG 3 cut(s) 1133, 1403, 3506
PpsI GAGTC 5 cut(s) 1334, 2507, 2986, 3341, 3942
Ppu21I YACGTR 3 cut(s) 1133, 1403, 3506
PpuMI RGGWCCY 2 cut(s) 2658, 3182
PscI ACATGT 3 cut(s) 2197, 3065, 4134
PshAI GACNNNNGTC 2 cut(s) 41, 341
PsiI TTATAA 1 cut(s) 3456
Psp124BI GAGCTC 3 cut(s) 738, 2211, 3367
Psp5II RGGWCCY 2 cut(s) 2658, 3182
Psp6I CCWGG 3 cut(s) 2236, 2995, 3228
PspCI CACGTG 3 cut(s) 1133, 1403, 3506
PspFI CCCAGC 2 cut(s) 2386, 3713
PspGI CCWGG 3 cut(s) 2236, 2995, 3228
PspN4I GGNNCC 6 cut(s) 233, 1880, 2098, 3054, 3183, 3653
PspPI GGNCC 6 cut(s) 1406, 1879, 2658, 3053, 3182, 3916
PspPPI RGGWCCY 2 cut(s) 2658, 3182
PstI CTGCAG 1 cut(s) 1924
PstNI CAGNNNCTG 1 cut(s) 2135
PsuI RGATCY 5 cut(s) 367, 674, 1417, 2183, 4037
PvuII CAGCTG 2 cut(s) 641, 2132
RsaI GTAC 6 cut(s) 860, 1014, 2526, 2808, 3751, 4087
RsaNI GTAC 6 cut(s) 859, 1013, 2525, 2807, 3750, 4086
RseI CAYNNNNRTG 5 cut(s) 57, 1652, 2307, 3420, 3504
SacI GAGCTC 3 cut(s) 738, 2211, 3367
SalI GTCGAC 1 cut(s) 68
SapI GCTCTTC 2 cut(s) 430, 1566
Sau3AI GATC 9 cut(s) 367, 674, 1417, 2183, 2749, 2778, 3412, 3971, 4037
Sau96I GGNCC 6 cut(s) 1406, 1879, 2658, 3053, 3182, 3916
ScaI AGTACT 2 cut(s) 860, 3751
SchI GAGTC 5 cut(s) 1335, 2507, 2986, 3342, 3943
ScrFI CCNGG 3 cut(s) 2238, 2997, 3230
SduI GDGCHC 9 cut(s) 93, 738, 959, 1765, 1852, 2211, 3151, 3228, 3367
SfcI CTRYAG 5 cut(s) 630, 1902, 1920, 2520, 4077
SfoI GGCGCC 1 cut(s) 233
SgrDI CGTCGACG 1 cut(s) 68
SinI GGWCC 6 cut(s) 1406, 1879, 2658, 3053, 3182, 3916
SmiMI CAYNNNNRTG 5 cut(s) 57, 1652, 2307, 3420, 3504
SmlI CTYRAG 5 cut(s) 883, 1565, 2651, 3693, 4099
SmoI CTYRAG 5 cut(s) 883, 1565, 2651, 3693, 4099
SsiI CCGC 8 cut(s) 328, 527, 908, 2078, 2384, 2883, 3303, 4045
SspDI GGCGCC 1 cut(s) 231
SspI AATATT 2 cut(s) 1602, 2113
SspMI CTAG 5 cut(s) 278, 371, 599, 2433, 3486
SstI GAGCTC 3 cut(s) 738, 2211, 3367
StyD4I CCNGG 3 cut(s) 2236, 2995, 3228
StyI CCWWGG 2 cut(s) 2064, 3048
TaiI ACGT 8 cut(s) 69, 132, 1135, 1239, 1405, 1996, 2728, 3508
TaqI TCGA 8 cut(s) 57, 69, 120, 404, 1278, 1542, 1810, 3331
TatI WGTACW 5 cut(s) 858, 1012, 2524, 2806, 3749
TauI GCSGC 3 cut(s) 530, 2080, 2885
TscAI CASTG 6 cut(s) 109, 1168, 2173, 3312, 3586, 3868
TseFI GTSAC 6 cut(s) 655, 1028, 1327, 1365, 2510, 3513
Tsp45I GTSAC 6 cut(s) 655, 1028, 1327, 1365, 2510, 3513
TspRI CASTG 6 cut(s) 109, 1168, 2173, 3312, 3586, 3868
VneI GTGCAC 2 cut(s) 1761, 3224
VpaK11BI GGWCC 6 cut(s) 1406, 1879, 2658, 3053, 3182, 3916
XagI CCTNNNNNAGG 1 cut(s) 3105
XapI RAATTY 7 cut(s) 826, 1039, 1396, 1531, 1940, 2037, 3734
XceI RCATGY 8 cut(s) 1019, 1351, 1549, 2201, 2889, 3069, 3328, 4138
XcmI CCANNNNNNNNNTGG 1 cut(s) 2269
XmiI GTMKAC 2 cut(s) 69, 1905
XspI CTAG 5 cut(s) 278, 371, 599, 2433, 3486
ZraI GACGTC 1 cut(s) 1994
ZrmI AGTACT 2 cut(s) 860, 3751
Zsp2I ATGCAT 4 cut(s) 610, 1551, 1819, 4027
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.