MD12G1041200.v1.1

Protein virilizer homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0010141
Physical Location & Seq
Reverse (-)
4570418 .. 4573051
2634 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1041200.v1.1.491

Sequence Viewer

Length: 1272 bp
ATGTCAGTATCACGTCCTGAAATTCCATTGACTCGTGAACCCAGTGTTTCTTCAGATAAAAAGTACTTTGAGCATTCTGACGACTCGAAGACTGCTATACTACACAAGGCTTCTAGTGGTTTTGACTCTGCAACAACAGCAAATAGTCCTAGATTCCCTGTTTTTGCTTATAATAACTCTGCAGCATCTTCCATACAGATACCAGTTGATTCTAGGATGACTCCACAAAATTTCTTTCCAAAGAATAGTCCGCAACATGCTGGCAATGTGCCTCCAGGATTTTATGACCAGAGATTTCTTCCAAATCAACCTCCTTTACCTCCCATGCCACCTCCCTCAACAGCTGTGATATCGCAGACTTCTGATTCTGTTCCAAGTCAATCATCCCCTTTTATGAACTCTATGACTGATGTACAGCAGTCACTTCCGACACCCTTTCAGATTCGCTCAGATTATCTTTCTCCCTTCAATAATGGTTCCACATCTTCAAGAAATTTCATCTCTTCTCCTAGTGGGGCTGTCAGACCACCTCCACCACTTCCTCCTACACCACCTCCTTTTTCCTCTAGTCCATATAACTTAACGCCCAATAGAACTATTACACAGTCTTCAGTATATAATCAGACAAGTGTGGGAACAACTGAACTTCTTCATAGCTCTACTACACCTTCAGGGGCCAGAGTAAATACCTACTCGCCATCTCCGCACATGGTTTTCCGACCTGGTTCTAATTCTATGAGTCCTTATGGAAGCATCCCAACTCAGCTGCAAGGCGACAATGCAACTGTTTTGCAGAATCTCTCTATCGCTCAGTCTTCCATTCAGGCAATTCATTCCCTTGCTCAGTTGCAGCCGCTGCAGCCACCACAGGTTCCACGCCCTCCACAACCACCTCAGCATCTTAGGCCACCTATGCAAGCTTCACAACAGTTGGAGCAAGGTGTATCTATGCAGAACCAAGTTCAAATGCATTCACTACAGATTCTGCAACAGCCACATGTTTCACCTATGCATGCTTACTATCAATCCCAACAGCAGGAGTTTGCTCATGTACAGCAGCAACAGCAAGTTGACCATTCTCAACAGCAGGCAATGCATCAATCAGGGGATGGTACATCCCAGCAACAAGATCCAGCAATGTCGTTACATGAATACTTCAAGTCTCCAGAAGCTATTCAGTCTTTATTGAGTGACCGAGATAAACTTTGTCAGCTTTTGGAGCAGCATCCAAAGTTAATGCAGATGCTTGAGGAGAAGTTGGGCCAGATATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
VIR_N

Protein Analysis

424

Amino Acids

46.56

Weight (kDa)

6.56

Isoelectric Point (pI)

83.37

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010141)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G05680 AT3G05680
fragaria_vesca FvH4_6g20410 FvH4_6g20410 FvH4_6g20410
malus_domestica MD12G1041200.v1.1 MD12G1041400.v1.1 MD14G1040500.v1.1
prunus_persica Prupe.7G093800_v2.0.a1
pyrus_communis pycom12g03590 pycom14g03440
rosa_chinensis RchiOBHm_Chr3g0476141
rosa_laevigata RLG00000023788
rosa_multiflora Rmu_sc0000114.1_g000025
rosa_roxburghii Rroxscaffold_6G00405450
rosa_rugosa Rorug03G0153000
rosa_samantha Rh3AG204100 Rh3BG234300 Rh3CG229700 Rh3DG229700
rosa_wichuraiana Rw3G018550

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 171
AciI CCGC 3 cut(s) 251, 704, 854
AclWI GGATC 1 cut(s) 1124
AcsI RAATTY 3 cut(s) 21, 229, 493
AcuI CTGAAG 3 cut(s) 36, 594, 654
AfaI GTAC 4 cut(s) 65, 414, 1053, 1114
AfiI CCNNNNNNNGG 1 cut(s) 1036
AflIII ACRYGT 1 cut(s) 997
AgsI TTSAA 4 cut(s) 469, 489, 965, 1159
AjiI CACGTC 1 cut(s) 14
AjnI CCWGG 2 cut(s) 274, 721
AluBI AGCT 6 cut(s) 344, 657, 766, 920, 1172, 1213
AluI AGCT 6 cut(s) 344, 657, 766, 920, 1172, 1213
Alw26I GTCTC 1 cut(s) 1167
AlwI GGATC 1 cut(s) 1124
AlwNI CAGNNNCTG 2 cut(s) 856, 985
AoxI GGCC 3 cut(s) 675, 905, 1261
ApeKI GCWGC 7 cut(s) 182, 766, 850, 856, 859, 1057, 1222
ApoI RAATTY 3 cut(s) 21, 229, 493
Asp700I GAANNNNTTC 2 cut(s) 648, 1173
AspS9I GGNCC 2 cut(s) 675, 1261
AsuHPI GGTGA 1 cut(s) 996
BauI CACGAG 1 cut(s) 33
BbsI GAAGAC 3 cut(s) 95, 600, 807
BbvCI CCTCAGC 1 cut(s) 894
BbvI GCAGC 7 cut(s) 194, 753, 843, 862, 871, 1069, 1234
BccI CCATC 2 cut(s) 706, 1103
BciT130I CCWGG 2 cut(s) 276, 723
BcoDI GTCTC 1 cut(s) 1167
BfaI CTAG 5 cut(s) 114, 150, 213, 510, 567
BfmI CTRYAG 3 cut(s) 180, 857, 977
BisI GCNGC 8 cut(s) 183, 767, 851, 854, 857, 860, 1058, 1223
BlsI GCNGC 8 cut(s) 184, 768, 852, 855, 858, 861, 1059, 1224
BmcAI AGTACT 1 cut(s) 65
Bme1390I CCNGG 2 cut(s) 276, 723
BmgBI CACGTC 1 cut(s) 14
BmgT120I GGNCC 2 cut(s) 675, 1261
BmiI GGNNCC 3 cut(s) 478, 676, 873
BmrFI CCNGG 2 cut(s) 276, 723
BmrI ACTGGG 1 cut(s) 36
BmsI GCATC 6 cut(s) 194, 762, 907, 1105, 1233, 1234
BmuI ACTGGG 1 cut(s) 36
BpiI GAAGAC 3 cut(s) 95, 600, 807
BpmI CTGGAG 2 cut(s) 258, 1149
Bpu10I CCTNAGC 1 cut(s) 894
BpuEI CTTGAG 1 cut(s) 1268
BsaXI ACNNNNNCTCC 2 cut(s) 538, 568
Bsc4I CCNNNNNNNGG 1 cut(s) 1036
Bse1I ACTGG 2 cut(s) 42, 203
Bse3DI GCAATG 3 cut(s) 271, 1098, 1143
BseBI CCWGG 2 cut(s) 276, 723
BseGI GGATG 6 cut(s) 222, 383, 753, 1114, 1115, 1225
BseLI CCNNNNNNNGG 1 cut(s) 1036
BseMI GCAATG 3 cut(s) 271, 1098, 1143
BseMII CTCAG 5 cut(s) 462, 776, 824, 857, 908
BseNI ACTGG 2 cut(s) 42, 203
BseRI GAGGAG 1 cut(s) 1265
BseXI GCAGC 7 cut(s) 194, 753, 843, 862, 871, 1069, 1234
BseYI CCCAGC 1 cut(s) 1119
BshFI GGCC 3 cut(s) 677, 907, 1263
BslI CCNNNNNNNGG 1 cut(s) 1036
BsmAI GTCTC 1 cut(s) 1167
BsmI GAATGC 2 cut(s) 73, 970
BsnI GGCC 3 cut(s) 677, 907, 1263
Bsp1407I TGTACA 2 cut(s) 412, 1051
Bsp143I GATC 1 cut(s) 1129
BspACI CCGC 3 cut(s) 251, 704, 854
BspANI GGCC 3 cut(s) 677, 907, 1263
BspCNI CTCAG 5 cut(s) 461, 775, 823, 856, 907
BspLI GGNNCC 3 cut(s) 478, 676, 873
BspMAI CTGCAG 2 cut(s) 184, 861
BspPI GGATC 1 cut(s) 1124
BsrDI GCAATG 3 cut(s) 271, 1098, 1143
BsrGI TGTACA 2 cut(s) 412, 1051
BsrI ACTGG 2 cut(s) 42, 203
BssMI GATC 1 cut(s) 1129
BssSI CACGAG 1 cut(s) 33
Bst2BI CACGAG 1 cut(s) 33
Bst2UI CCWGG 2 cut(s) 276, 723
Bst4CI ACNGT 3 cut(s) 606, 787, 930
Bst6I CTCTTC 1 cut(s) 508
BstAPI GCANNNNNTGC 2 cut(s) 856, 1093
BstAUI TGTACA 2 cut(s) 412, 1051
BstC8I GCNNGC 4 cut(s) 262, 918, 1014, 1089
BstDEI CTNAG 6 cut(s) 448, 762, 810, 843, 894, 902
BstF5I GGATG 6 cut(s) 222, 383, 753, 1114, 1115, 1225
BstKTI GATC 1 cut(s) 1132
BstMAI GTCTC 1 cut(s) 1167
BstMBI GATC 1 cut(s) 1129
BstMWI GCNNNNNNNGC 9 cut(s) 137, 703, 856, 859, 904, 913, 1063, 1093, 1219
BstNI CCWGG 2 cut(s) 276, 723
BstNSI RCATGY 3 cut(s) 260, 1001, 1016
BstSCI CCNGG 2 cut(s) 274, 721
BstSFI CTRYAG 3 cut(s) 180, 857, 977
BstV1I GCAGC 7 cut(s) 194, 753, 843, 862, 871, 1069, 1234
BstV2I GAAGAC 3 cut(s) 95, 600, 807
BstX2I RGATCY 1 cut(s) 1129
BstYI RGATCY 1 cut(s) 1129
BsuRI GGCC 3 cut(s) 677, 907, 1263
BtrI CACGTC 1 cut(s) 14
BtsCI GGATG 6 cut(s) 222, 383, 753, 1114, 1115, 1225
BtsIMutI CAGTG 1 cut(s) 49
Cac8I GCNNGC 4 cut(s) 262, 918, 1014, 1089
CaiI CAGNNNCTG 2 cut(s) 856, 985
Cfr13I GGNCC 2 cut(s) 675, 1261
CsiI ACCWGGT 1 cut(s) 721
Csp6I GTAC 4 cut(s) 64, 413, 1052, 1113
CviAII CATG 7 cut(s) 257, 325, 709, 998, 1013, 1049, 1148
CviQI GTAC 4 cut(s) 64, 413, 1052, 1113
DdeI CTNAG 6 cut(s) 448, 762, 810, 843, 894, 902
DpnI GATC 1 cut(s) 1131
DpnII GATC 1 cut(s) 1129
Eam1104I CTCTTC 1 cut(s) 508
EarI CTCTTC 1 cut(s) 508
Eco32I GATATC 1 cut(s) 351
Eco57I CTGAAG 3 cut(s) 36, 594, 654
EcoRII CCWGG 2 cut(s) 274, 721
EcoRV GATATC 1 cut(s) 351
EcoT22I ATGCAT 3 cut(s) 972, 1014, 1098
FaeI CATG 7 cut(s) 260, 328, 712, 1001, 1016, 1052, 1151
FatI CATG 7 cut(s) 256, 324, 708, 997, 1012, 1048, 1147
Fnu4HI GCNGC 8 cut(s) 183, 767, 851, 854, 857, 860, 1058, 1223
FokI GGATG 6 cut(s) 229, 370, 740, 1102, 1121, 1212
Fsp4HI GCNGC 8 cut(s) 183, 767, 851, 854, 857, 860, 1058, 1223
FspBI CTAG 5 cut(s) 114, 150, 213, 510, 567
GluI GCNGC 8 cut(s) 183, 767, 851, 854, 857, 860, 1058, 1223
GsaI CCCAGC 1 cut(s) 1123
GsuI CTGGAG 2 cut(s) 258, 1149
HaeIII GGCC 3 cut(s) 677, 907, 1263
Hin1II CATG 7 cut(s) 260, 328, 712, 1001, 1016, 1052, 1151
HincII GTYRAC 1 cut(s) 1072
HindII GTYRAC 1 cut(s) 1072
HindIII AAGCTT 1 cut(s) 918
HphI GGTGA 1 cut(s) 996
Hpy166II GTNNAC 2 cut(s) 38, 1072
Hpy188I TCNGA 9 cut(s) 55, 79, 364, 429, 441, 451, 524, 624, 719
Hpy188III TCNNGA 4 cut(s) 17, 35, 489, 1166
Hpy8I GTNNAC 2 cut(s) 38, 1072
HpyAV CCTTC 2 cut(s) 475, 678
HpyCH4III ACNGT 3 cut(s) 606, 787, 930
HpyCH4IV ACGT 1 cut(s) 13
HpyF10VI GCNNNNNNNGC 9 cut(s) 137, 703, 856, 859, 904, 913, 1063, 1093, 1219
HpyF3I CTNAG 6 cut(s) 448, 762, 810, 843, 894, 902
HpySE526I ACGT 1 cut(s) 13
Hsp92II CATG 7 cut(s) 260, 328, 712, 1001, 1016, 1052, 1151
Kzo9I GATC 1 cut(s) 1129
LmnI GCTCC 2 cut(s) 934, 1219
Lsp1109I GCAGC 7 cut(s) 194, 753, 843, 862, 871, 1069, 1234
LweI GCATC 6 cut(s) 194, 762, 907, 1105, 1233, 1234
MabI ACCWGGT 1 cut(s) 721
MaeI CTAG 5 cut(s) 114, 150, 213, 510, 567
MaeII ACGT 1 cut(s) 13
MaeIII GTNAC 3 cut(s) 420, 1143, 1190
MalI GATC 1 cut(s) 1131
MboI GATC 1 cut(s) 1129
MboII GAAGA 9 cut(s) 42, 100, 180, 290, 477, 495, 600, 641, 807
MflI RGATCY 1 cut(s) 1129
MluCI AATT 5 cut(s) 21, 229, 493, 730, 828
MlyI GAGTC 5 cut(s) 25, 77, 119, 214, 748
MmeI TCCRAC 3 cut(s) 452, 742, 912
Mph1103I ATGCAT 3 cut(s) 972, 1014, 1098
MroXI GAANNNNTTC 2 cut(s) 648, 1173
MseI TTAA 2 cut(s) 581, 1235
MspA1I CMGCKG 3 cut(s) 344, 766, 856
MspR9I CCNGG 2 cut(s) 276, 723
Mva1269I GAATGC 2 cut(s) 73, 970
MvaI CCWGG 2 cut(s) 276, 723
MwoI GCNNNNNNNGC 9 cut(s) 137, 703, 856, 859, 904, 913, 1063, 1093, 1219
NdeII GATC 1 cut(s) 1129
NlaIII CATG 7 cut(s) 260, 328, 712, 1001, 1016, 1052, 1151
NlaIV GGNNCC 3 cut(s) 478, 676, 873
NmuCI GTSAC 2 cut(s) 420, 1190
NsiI ATGCAT 3 cut(s) 972, 1014, 1098
NspI RCATGY 3 cut(s) 260, 1001, 1016
PaeI GCATGC 1 cut(s) 1016
PciI ACATGT 1 cut(s) 997
PctI GAATGC 2 cut(s) 73, 970
PdmI GAANNNNTTC 2 cut(s) 648, 1173
PfeI GAWTC 6 cut(s) 153, 209, 365, 442, 796, 982
PfoI TCCNGGA 1 cut(s) 274
PkrI GCNGC 8 cut(s) 184, 768, 852, 855, 858, 861, 1059, 1224
PleI GAGTC 5 cut(s) 25, 77, 119, 214, 747
PpsI GAGTC 5 cut(s) 25, 77, 119, 214, 747
PscI ACATGT 1 cut(s) 997
PsiI TTATAA 1 cut(s) 171
Psp6I CCWGG 2 cut(s) 274, 721
PspFI CCCAGC 1 cut(s) 1119
PspGI CCWGG 2 cut(s) 274, 721
PspN4I GGNNCC 3 cut(s) 478, 676, 873
PspPI GGNCC 2 cut(s) 675, 1261
PstI CTGCAG 2 cut(s) 184, 861
PstNI CAGNNNCTG 2 cut(s) 856, 985
PsuI RGATCY 1 cut(s) 1129
PvuII CAGCTG 2 cut(s) 344, 766
RsaI GTAC 4 cut(s) 65, 414, 1053, 1114
RsaNI GTAC 4 cut(s) 64, 413, 1052, 1113
SaqAI TTAA 2 cut(s) 581, 1235
SatI GCNGC 8 cut(s) 183, 767, 851, 854, 857, 860, 1058, 1223
Sau3AI GATC 1 cut(s) 1129
Sau96I GGNCC 2 cut(s) 675, 1261
ScaI AGTACT 1 cut(s) 65
SchI GAGTC 5 cut(s) 25, 77, 119, 214, 748
ScrFI CCNGG 2 cut(s) 276, 723
SexAI ACCWGGT 1 cut(s) 721
SfaNI GCATC 6 cut(s) 194, 762, 907, 1105, 1233, 1234
SfcI CTRYAG 3 cut(s) 180, 857, 977
SmlI CTYRAG 1 cut(s) 1247
SmoI CTYRAG 1 cut(s) 1247
SphI GCATGC 1 cut(s) 1016
Sse9I AATT 5 cut(s) 21, 229, 493, 730, 828
SsiI CCGC 3 cut(s) 251, 704, 854
SspMI CTAG 5 cut(s) 114, 150, 213, 510, 567
StyD4I CCNGG 2 cut(s) 274, 721
TaaI ACNGT 3 cut(s) 606, 787, 930
TaiI ACGT 1 cut(s) 16
TaqI TCGA 1 cut(s) 86
TaqII GACCGA 1 cut(s) 1209
TasI AATT 5 cut(s) 21, 229, 493, 730, 828
TatI WGTACW 3 cut(s) 63, 412, 1051
TauI GCSGC 1 cut(s) 856
TfiI GAWTC 6 cut(s) 153, 209, 365, 442, 796, 982
Tru1I TTAA 2 cut(s) 581, 1235
Tru9I TTAA 2 cut(s) 581, 1235
TscAI CASTG 1 cut(s) 49
TseFI GTSAC 2 cut(s) 420, 1190
TseI GCWGC 7 cut(s) 182, 766, 850, 856, 859, 1057, 1222
Tsp45I GTSAC 2 cut(s) 420, 1190
TspDTI ATGAA 5 cut(s) 410, 487, 641, 821, 1164
TspRI CASTG 1 cut(s) 49
XapI RAATTY 3 cut(s) 21, 229, 493
XceI RCATGY 3 cut(s) 260, 1001, 1016
XmnI GAANNNNTTC 2 cut(s) 648, 1173
XspI CTAG 5 cut(s) 114, 150, 213, 510, 567
ZrmI AGTACT 1 cut(s) 65
Zsp2I ATGCAT 3 cut(s) 972, 1014, 1098
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.