AT3G07400

lipase class 3 family protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
3
OG0007769
Physical Location & Seq
Forward (+)
2367222 .. 2372445
5224 bp
Loading structure...
UTR
Exon/CDS
Intron
AT3G07400.1

Sequence Viewer

Length: 3012 bp
ATGGAGTCGATACAGAGCCGAGTGGAATCGTGGATCAGAGACCAGAGGGCAAGGTTCTTACGGGTTTCGTGGGGACCGATTCAATGGAGGTTCCGGTGGCCACCATGGAACGGAGGAGATGCGGATCAAAGGATTAAGATCCGTCGGGAATACGAGAAGCGCAAAAAGCAAATTGAGGATCTCTGTCTCGCACTTAAATCAGAGTCTGTCGAAGATTTACAAGACATACTCTGTTGCATGGTACTCTCCGAGTGTGTTTACAAGAGACCTGCAAGTGAGATGGTTCGAGCTGTTAACAAATTTAAAGCGGACTTCGGTGGCCAATTTATTTCGTTGGAGAGAGTTCAACCTTCATCAGATCACGTTCCTCACAGGTATCTACTTGCTGAGGCAGGTGATACATTGTTTGCATCCTTTGTTGGAACTAGGCAATATAAGGACATCATGGCCGATGCAAATATACTTCAAGGACACATATTTCACGATGATGTCGCAGAGGATGAATGTATCGAAGCATCTGAGCCTATACAGAGTGAACCACTTAAGAATAATGGAGAGGGTTTGCGGAATCCTAAGCAACTTAGACAGAAGCCCAAACCTGCTGCTCATCGGGGGTTCTTGGCTCGTGCAAAGGGGATTCCTGCTCTGGAACTTTATAGGCTTGCTCAGAAAAAGAAACGAAAACTTGTATTATGTGGACACTCACTTGGTGGAGCTGTAGCTGCGTTGGCTACACTCGCCATATTGAGGGTTGTTGCTGCATCTTCAAAGAGAGGAAATGAAAATATTCATGTCAAATGTATAACCTTTTCCCAACCTCCTGTTGGAAATGCTGCGTTGAGAGATTATGTTCATGAAAAAGGGTGGCATCATTATTTCAAAAGCTACTGCATTCCTGAAGATTTGGTCCCGAGGATATTATCTCCTGCATATTTTCATCATTACAATGAACAAAGAATCTCAATGGCTGGAGAGACTGAAGCCACAAATGGCCAGGGCGTAACATCAGAGGCAGAAAAGAGAAAAACTAAGGAGCACGAGCAGTTGGTCATTGGTGTTGGCCCTGTTCAGAACTCATTCTGGAGGCTTTCAAAACTTGTCCCCCTAGAGGCTGTTAAGAAACAATTGGATAGGTACATAGGAAAGAAAGAGGATCCTGCAGAGACGTCTACTGCAAATGAATCCGCAGTGTTAGCTCCTATTAGAGACGTGGTTATTGAACCACAATCTCTTGAAATAGAAGAGGGTAAGGATGGTATATCTCTCAAGCCATTGCCAGATGCTGGTAATGGACCAACAGTTAGTGGAAGAAGTGGAGGAAAGACCAATTCGCCTAATGGGTTCAGGGTTCCTTATCTACCCTCTTACGTTCCATTTGGAGAGCTATATCTCTTGGGAACTGCATCTGTAGAATCACTTTCAGAAGGAGAGTACTCTAAATTGACATCGGTCAGATCTGTGATAACCGAACTGAGAGAACGTCTTCAGTCACATTCTATGAAGTCCTATAGGTCTCGCTTCCAGAGAATACATGACCTCTGCATGGATGTTGATGGTTTTTTTGGAGTGGATCAACAGAAGCAGTTTCCACATCTACAACAGTGGCTTGGACTTGCTGTCGGTGGTAGCATAGAACTTGGGCACATTGTTGAATCACCGGTTATTAGAACCGCCACTTCTATCGCTCCTCTTGGCTGGAAAGGCGTTCCGGGTGATAAGAATGCAGAACTTCTAAAGGTTGATATCACTGGATTTGGCTTGCATTTGTGTTCTTTTGTACATGCACAAGTGAATGGCAACTGGTGCTCAACTACTGTGGAATCTTTCCCTACAACACCTGCTTATTCTTCAGATAATGTAGAACAGACAGAACTACAGAAAATTAGGGTGGTCATTGGAGCTCCTCTGAAACGACCTCCAAGCAATCAGATAGTTGAAGATCCTTTGGTTCCTATGTTCTCGTCTGTTGATTCGAAAACCGGTTTTCCAAAGGAAGGAATCAACCTAGGATTTTTTCAAGAGGACAAGTTTGTCCGTCCTGAAGGTTTGGAAGACTTGTATATATTCTGCACTAGTGATTTCGCTACTGTAGCTAAGGAAGTTGAGGTCAGAACACGGAGGGTGAGACTACTCGGGCTTGAGGGTGCTGGTAAAACCTCTCTATTCCGCGCTATACTGGGCCAAAGCATGCTAAGTTCGATGACTCATGTGGAAAATCTTCAGATACAATCTGATGTTCAAGAATGTATTATTGGTGGTGTTTGCTACTCGGACACTGTGGGCGTAAACCTGCAGGAGCTGCACTTAGAGGCATCTCGCTTCAGAGAAGAACTATGGAAAGGAGTTCGTAATCTAAGTAAAAAGATAGATTTAATTATTCTTGTGCACAATCTCTCTCACAGAATACCACGTTACCAAAACTCAACAACACTACTGCAGCAACAGCAACCAGCACTTGCTCTTCTGTTAGATGAGGTTAAATCTCTTGGTATCCCTTGGGTCCTCGCCATAACAAACAAGTTCTCAGTCAGTGCCCATCAGCAGAAATCCGCTATCGAAGCTGTACTTCAGGCATACCAAGCGTCTCCAAACACAACCGGAATTGTCAACTCTATCCCTTATATTATTTCTGGCTCTGGAACTTCCTCATTGCCTTGGGCAGCTGTGAATGCGGGAAATGACGGATCAGTAGGCGTACCGAAGATGATATTCGCTCCACTCGACCTTGTCAAAAAGCCGTTTCAGAGAAAAGACACTGTTTTTCCAGTGGATGGTGTGAATTCTCTTTGCGAGCTTGTCCATCGTGTCCTTCAAACTCAAGAAGAAGCTTGCTTTGAGGAACTTGCTAGAGACAGACTGCTGGTGGAACTAGCCAAAGACCGTGTCGTAGATGGTTCACAGGCCAAGTCATCATCAATGTCAGCAGCAGCTGTAGGTGCTTCACTTGGAGCTGGTCTTGGCCTTGTCTTGGCAGTAGTAATGGGTGCTGGTTCCGCTTTACGAAAACCCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_3

Protein Analysis

1003

Amino Acids

110.86

Weight (kDa)

8.17

Isoelectric Point (pI)

47.94

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_3 PF01764 199 - 306 4.7e-15 Lipase (class 3)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 2 cut(s) 383, 1846
AasI GACNNNNNNGTC 1 cut(s) 2030
AatII GACGTC 1 cut(s) 1169
Acc36I ACCTGC 5 cut(s) 277, 383, 607, 1846, 2298
AccB7I CCANNNNNTGG 2 cut(s) 1283, 2771
AccI GTMKAC 1 cut(s) 1169
AccII CGCG 1 cut(s) 2169
AciI CCGC 9 cut(s) 122, 308, 565, 1185, 1671, 2167, 2550, 2672, 2994
AclWI GGATC 9 cut(s) 41, 132, 133, 186, 1148, 1161, 1578, 1934, 2692
AcoI YGGCCR 4 cut(s) 98, 319, 447, 991
AcsI RAATTY 2 cut(s) 299, 2779
AcuI CTGAAG 8 cut(s) 918, 999, 1469, 1833, 2061, 2204, 2305, 2552
AcyI GRCGYC 1 cut(s) 1166
AdeI CACNNNGTG 1 cut(s) 710
AfaI GTAC 6 cut(s) 243, 1136, 1433, 1779, 2565, 2697
AfiI CCNNNNNNNGG 9 cut(s) 110, 747, 824, 1108, 1283, 1543, 1994, 2771, 2968
AflII CTTAAG 1 cut(s) 542
AgeI ACCGGT 2 cut(s) 1657, 1979
AhdI GACNNNNNGTC 1 cut(s) 1616
AhlI ACTAGT 1 cut(s) 2072
AjiI CACGTC 1 cut(s) 1210
AjnI CCWGG 1 cut(s) 993
AjuI GAANNNNNNNTTGG 2 cut(s) 2235, 2267
Alw21I GWGCWC 4 cut(s) 1038, 1808, 1903, 2388
Alw44I GTGCAC 1 cut(s) 2384
AlwI GGATC 9 cut(s) 41, 132, 133, 186, 1148, 1161, 1578, 1934, 2692
AlwNI CAGNNNCTG 4 cut(s) 206, 1283, 2299, 2930
Ama87I CYCGRG 2 cut(s) 910, 2132
AoxI GGCC 8 cut(s) 98, 319, 447, 991, 1060, 2179, 2901, 2959
ApaLI GTGCAC 1 cut(s) 2384
ApeKI GCWGC 9 cut(s) 602, 722, 758, 833, 2299, 2437, 2660, 2924, 2927
ApoI RAATTY 2 cut(s) 299, 2779
ArsI GACNNNNNNTTYG 2 cut(s) 2868, 2900
AsiGI ACCGGT 2 cut(s) 1657, 1979
Asp700I GAANNNNTTC 4 cut(s) 786, 1076, 1482, 2217
AspA2I CCTAGG 1 cut(s) 2005
AspLEI GCGC 2 cut(s) 162, 2171
AspS9I GGNCC 6 cut(s) 74, 907, 1061, 1292, 2179, 2500
AsuC2I CCSGG 1 cut(s) 1710
AsuHPI GGTGA 4 cut(s) 407, 1647, 1724, 2134
AsuII TTCGAA 1 cut(s) 1973
AvaI CYCGRG 2 cut(s) 910, 2132
AvaII GGWCC 4 cut(s) 74, 907, 1292, 2500
AvrII CCTAGG 1 cut(s) 2005
BaeGI GKGCMC 3 cut(s) 1644, 2388, 2536
BalI TGGCCA 3 cut(s) 100, 321, 993
BamHI GGATCC 1 cut(s) 1153
BanII GRGCYC 1 cut(s) 1903
BauI CACGAG 2 cut(s) 624, 1037
BbsI GAAGAC 2 cut(s) 1475, 2058
Bbv12I GWGCWC 4 cut(s) 1038, 1808, 1903, 2388
BbvCI CCTCAGC 1 cut(s) 387
BbvI GCAGC 9 cut(s) 589, 709, 745, 820, 2286, 2449, 2672, 2936, 2939
BccI CCATC 7 cut(s) 274, 1247, 1547, 2544, 2765, 2808, 2885
BceAI ACGGC 1 cut(s) 2722
BciT130I CCWGG 1 cut(s) 995
BciVI GTATCC 1 cut(s) 2501
BcnI CCSGG 1 cut(s) 1710
BcuI ACTAGT 1 cut(s) 2072
BfaI CTAG 6 cut(s) 426, 1106, 2006, 2073, 2847, 2870
BfmI CTRYAG 9 cut(s) 717, 1158, 1407, 1507, 1874, 2088, 2291, 2435, 2931
BfrI CTTAAG 1 cut(s) 542
BfuAI ACCTGC 5 cut(s) 277, 383, 607, 1846, 2298
BfuI GTATCC 1 cut(s) 2501
BglII AGATCT 1 cut(s) 1454
BisI GCNGC 9 cut(s) 603, 723, 759, 834, 2300, 2438, 2661, 2925, 2928
BlnI CCTAGG 1 cut(s) 2005
BlsI GCNGC 9 cut(s) 604, 724, 760, 835, 2301, 2439, 2662, 2926, 2929
BmcAI AGTACT 1 cut(s) 1433
Bme1390I CCNGG 2 cut(s) 995, 1710
Bme18I GGWCC 4 cut(s) 74, 907, 1292, 2500
BmeRI GACNNNNNGTC 1 cut(s) 1616
BmeT110I CYCGRG 2 cut(s) 910, 2132
BmgBI CACGTC 1 cut(s) 1210
BmgT120I GGNCC 6 cut(s) 74, 907, 1061, 1292, 2179, 2500
BmiI GGNNCC 8 cut(s) 75, 92, 909, 1155, 1350, 1950, 2501, 2992
BmrFI CCNGG 2 cut(s) 995, 1710
BmrI ACTGGG 1 cut(s) 2186
BmsI GCATC 9 cut(s) 109, 419, 442, 524, 770, 877, 1270, 1412, 2321
BmuI ACTGGG 1 cut(s) 2186
BoxI GACNNNNGTC 1 cut(s) 1448
BpiI GAAGAC 2 cut(s) 1475, 2058
BpmI CTGGAG 2 cut(s) 990, 1102
Bpu10I CCTNAGC 3 cut(s) 387, 573, 2094
Bpu14I TTCGAA 1 cut(s) 1973
BpuEI CTTGAG 3 cut(s) 1250, 2159, 2802
BpuMI CCSGG 1 cut(s) 1710
BsaBI GATNNNNATC 2 cut(s) 123, 137
BsaHI GRCGYC 1 cut(s) 1166
BsaI GGTCTC 3 cut(s) 33, 259, 1518
BsaJI CCNNGG 6 cut(s) 104, 911, 994, 2005, 2495, 2654
BsaWI WCCGGW 4 cut(s) 93, 1657, 1979, 2597
Bsc4I CCNNNNNNNGG 9 cut(s) 110, 747, 824, 1108, 1283, 1543, 1994, 2771, 2968
Bse118I RCCGGY 2 cut(s) 1657, 1979
Bse1I ACTGG 4 cut(s) 1753, 1805, 2181, 2765
Bse3DI GCAATG 2 cut(s) 1271, 2648
Bse8I GATNNNNATC 2 cut(s) 123, 137
BseBI CCWGG 1 cut(s) 995
BseDI CCNNGG 6 cut(s) 104, 911, 994, 2005, 2495, 2654
BseGI GGATG 5 cut(s) 410, 505, 1258, 1552, 2776
BseJI GATNNNNATC 2 cut(s) 123, 137
BseLI CCNNNNNNNGG 9 cut(s) 110, 747, 824, 1108, 1283, 1543, 1994, 2771, 2968
BseMI GCAATG 2 cut(s) 1271, 2648
BseMII CTCAG 5 cut(s) 378, 510, 680, 1463, 2538
BseNI ACTGG 4 cut(s) 1753, 1805, 2181, 2765
BseRI GAGGAG 3 cut(s) 129, 1677, 1893
BseSI GKGCMC 3 cut(s) 1644, 2388, 2536
BseXI GCAGC 9 cut(s) 589, 709, 745, 820, 2286, 2449, 2672, 2936, 2939
BsgI GTGCAG 2 cut(s) 2053, 2285
Bsh1236I CGCG 1 cut(s) 2169
BshFI GGCC 8 cut(s) 100, 321, 449, 993, 1062, 2181, 2903, 2961
BshTI ACCGGT 2 cut(s) 1657, 1979
BsiHKAI GWGCWC 4 cut(s) 1038, 1808, 1903, 2388
BsiHKCI CYCGRG 2 cut(s) 910, 2132
BsiSI CCGG 5 cut(s) 94, 1658, 1709, 1980, 2598
BslFI GGGAC 3 cut(s) 87, 893, 1085
BslI CCNNNNNNNGG 9 cut(s) 110, 747, 824, 1108, 1283, 1543, 1994, 2771, 2968
BsmBI CGTCTC 3 cut(s) 1157, 1200, 2589
BsmFI GGGAC 3 cut(s) 87, 893, 1085
BsmI GAATGC 3 cut(s) 891, 1726, 2674
BsnI GGCC 8 cut(s) 100, 321, 449, 993, 1062, 2181, 2903, 2961
Bso31I GGTCTC 3 cut(s) 33, 259, 1518
BsoBI CYCGRG 2 cut(s) 910, 2132
Bsp119I TTCGAA 1 cut(s) 1973
Bsp1286I GDGCHC 6 cut(s) 1038, 1644, 1808, 1903, 2388, 2536
Bsp1407I TGTACA 1 cut(s) 1777
Bsp19I CCATGG 1 cut(s) 104
BspACI CCGC 9 cut(s) 122, 308, 565, 1185, 1671, 2167, 2550, 2672, 2994
BspANI GGCC 8 cut(s) 100, 321, 449, 993, 1062, 2181, 2903, 2961
BspCNI CTCAG 5 cut(s) 379, 511, 679, 1464, 2537
BspFNI CGCG 1 cut(s) 2169
BspHI TCATGA 1 cut(s) 853
BspLI GGNNCC 8 cut(s) 75, 92, 909, 1155, 1350, 1950, 2501, 2992
BspMAI CTGCAG 3 cut(s) 1162, 2295, 2439
BspMI ACCTGC 5 cut(s) 277, 383, 607, 1846, 2298
BspPI GGATC 9 cut(s) 41, 132, 133, 186, 1148, 1161, 1578, 1934, 2692
BspQI GCTCTTC 1 cut(s) 2466
BspT104I TTCGAA 1 cut(s) 1973
BspTI CTTAAG 1 cut(s) 542
BspTNI GGTCTC 3 cut(s) 33, 259, 1518
BsrDI GCAATG 2 cut(s) 1271, 2648
BsrFI RCCGGY 2 cut(s) 1657, 1979
BsrGI TGTACA 1 cut(s) 1777
BsrI ACTGG 4 cut(s) 1753, 1805, 2181, 2765
BssAI RCCGGY 2 cut(s) 1657, 1979
BssECI CCNNGG 6 cut(s) 104, 911, 994, 2005, 2495, 2654
BssNI GRCGYC 1 cut(s) 1166
BssSI CACGAG 2 cut(s) 624, 1037
BssT1I CCWWGG 4 cut(s) 104, 2005, 2495, 2654
Bst2BI CACGAG 2 cut(s) 624, 1037
Bst2UI CCWGG 1 cut(s) 995
Bst4CI ACNGT 7 cut(s) 1300, 1602, 1816, 2089, 2278, 2758, 2882
Bst6I CTCTTC 2 cut(s) 1236, 2466
BstACI GRCGYC 1 cut(s) 1166
BstAFI CTTAAG 1 cut(s) 542
BstAPI GCANNNNNTGC 2 cut(s) 1803, 2299
BstAUI TGTACA 1 cut(s) 1777
BstBI TTCGAA 1 cut(s) 1973
BstC8I GCNNGC 5 cut(s) 663, 1760, 2189, 2792, 2830
BstDSI CCRYGG 1 cut(s) 104
BstF5I GGATG 5 cut(s) 410, 505, 1258, 1552, 2776
BstFNI CGCG 1 cut(s) 2169
BstHHI GCGC 2 cut(s) 162, 2171
BstNI CCWGG 1 cut(s) 995
BstNSI RCATGY 2 cut(s) 1784, 2191
BstPAI GACNNNNGTC 1 cut(s) 1448
BstSCI CCNGG 2 cut(s) 993, 1708
BstSFI CTRYAG 9 cut(s) 717, 1158, 1407, 1507, 1874, 2088, 2291, 2435, 2931
BstSLI GKGCMC 3 cut(s) 1644, 2388, 2536
BstUI CGCG 1 cut(s) 2169
BstV1I GCAGC 9 cut(s) 589, 709, 745, 820, 2286, 2449, 2672, 2936, 2939
BstV2I GAAGAC 2 cut(s) 1475, 2058
BstX2I RGATCY 5 cut(s) 138, 178, 1153, 1454, 1939
BstYI RGATCY 5 cut(s) 138, 178, 1153, 1454, 1939
BsuI GTATCC 1 cut(s) 2501
BsuRI GGCC 8 cut(s) 100, 321, 449, 993, 1062, 2181, 2903, 2961
BtgI CCRYGG 1 cut(s) 104
BtrI CACGTC 1 cut(s) 1210
BtsCI GGATG 5 cut(s) 410, 505, 1258, 1552, 2776
BtsI GCAGTG 1 cut(s) 1194
BtsIMutI CAGTG 7 cut(s) 1194, 1607, 1746, 2274, 2536, 2754, 2772
BveI ACCTGC 5 cut(s) 277, 383, 607, 1846, 2298
Cac8I GCNNGC 5 cut(s) 663, 1760, 2189, 2792, 2830
CaiI CAGNNNCTG 4 cut(s) 206, 1283, 2299, 2930
CciI TCATGA 1 cut(s) 853
CfoI GCGC 2 cut(s) 162, 2171
Cfr10I RCCGGY 2 cut(s) 1657, 1979
Cfr13I GGNCC 6 cut(s) 74, 907, 1061, 1292, 2179, 2500
CseI GACGC 1 cut(s) 2571
Csp6I GTAC 6 cut(s) 242, 1135, 1432, 1778, 2564, 2696
CspAI ACCGGT 2 cut(s) 1657, 1979
CspCI CAANNNNNGTGG 2 cut(s) 1797, 1832
CviQI GTAC 6 cut(s) 242, 1135, 1432, 1778, 2564, 2696
DraI TTTAAA 1 cut(s) 304
DraIII CACNNNGTG 1 cut(s) 710
DrdI GACNNNNNNGTC 1 cut(s) 2030
DriI GACNNNNNGTC 1 cut(s) 1616
DseDI GACNNNNNNGTC 1 cut(s) 2030
EaeI YGGCCR 4 cut(s) 98, 319, 447, 991
Eam1104I CTCTTC 2 cut(s) 1236, 2466
Eam1105I GACNNNNNGTC 1 cut(s) 1616
EarI CTCTTC 2 cut(s) 1236, 2466
Ecl136II GAGCTC 1 cut(s) 1901
Eco130I CCWWGG 4 cut(s) 104, 2005, 2495, 2654
Eco24I GRGCYC 1 cut(s) 1903
Eco31I GGTCTC 3 cut(s) 33, 259, 1518
Eco32I GATATC 1 cut(s) 1744
Eco47I GGWCC 4 cut(s) 74, 907, 1292, 2500
Eco53kI GAGCTC 1 cut(s) 1901
Eco57I CTGAAG 8 cut(s) 918, 999, 1469, 1833, 2061, 2204, 2305, 2552
Eco88I CYCGRG 2 cut(s) 910, 2132
EcoICRI GAGCTC 1 cut(s) 1901
EcoO109I RGGNCCY 1 cut(s) 2500
EcoRI GAATTC 1 cut(s) 2779
EcoRII CCWGG 1 cut(s) 993
EcoRV GATATC 1 cut(s) 1744
EcoT14I CCWWGG 4 cut(s) 104, 2005, 2495, 2654
EcoT38I GRGCYC 1 cut(s) 1903
ErhI CCWWGG 4 cut(s) 104, 2005, 2495, 2654
Esp3I CGTCTC 3 cut(s) 1157, 1200, 2589
FalI AAGNNNNNCTT 2 cut(s) 2550, 2582
FaqI GGGAC 3 cut(s) 87, 893, 1085
FauI CCCGC 1 cut(s) 2665
FblI GTMKAC 1 cut(s) 1169
Fnu4HI GCNGC 9 cut(s) 603, 723, 759, 834, 2300, 2438, 2661, 2925, 2928
FokI GGATG 5 cut(s) 397, 512, 1265, 1559, 2783
FriOI GRGCYC 1 cut(s) 1903
Fsp4HI GCNGC 9 cut(s) 603, 723, 759, 834, 2300, 2438, 2661, 2925, 2928
FspBI CTAG 6 cut(s) 426, 1106, 2006, 2073, 2847, 2870
GlaI GCGC 2 cut(s) 161, 2170
GluI GCNGC 9 cut(s) 603, 723, 759, 834, 2300, 2438, 2661, 2925, 2928
GsuI CTGGAG 2 cut(s) 990, 1102
HaeIII GGCC 8 cut(s) 100, 321, 449, 993, 1062, 2181, 2903, 2961
HapII CCGG 5 cut(s) 94, 1658, 1709, 1980, 2598
HgaI GACGC 1 cut(s) 2571
HhaI GCGC 2 cut(s) 162, 2171
Hin1I GRCGYC 1 cut(s) 1166
Hin6I GCGC 2 cut(s) 160, 2169
HinP1I GCGC 2 cut(s) 160, 2169
HincII GTYRAC 2 cut(s) 295, 2608
HindII GTYRAC 2 cut(s) 295, 2608
HindIII AAGCTT 1 cut(s) 2826
HpaI GTTAAC 1 cut(s) 295
HpaII CCGG 5 cut(s) 94, 1658, 1709, 1980, 2598
HphI GGTGA 4 cut(s) 407, 1647, 1724, 2134
Hpy166II GTNNAC 9 cut(s) 259, 295, 536, 698, 1170, 2287, 2386, 2608, 2897
Hpy8I GTNNAC 9 cut(s) 259, 295, 536, 698, 1170, 2287, 2386, 2608, 2897
Hpy99I CGWCG 1 cut(s) 147
HpyAV CCTTC 5 cut(s) 360, 1418, 1988, 2036, 2819
HpyCH4III ACNGT 7 cut(s) 1300, 1602, 1816, 2089, 2278, 2758, 2882
HpyCH4IV ACGT 6 cut(s) 363, 1166, 1209, 1368, 1480, 2410
HpySE526I ACGT 6 cut(s) 363, 1166, 1209, 1368, 1480, 2410
Hsp92I GRCGYC 1 cut(s) 1166
HspAI GCGC 2 cut(s) 160, 2169
KspAI GTTAAC 1 cut(s) 295
LguI GCTCTTC 1 cut(s) 2466
LmnI GCTCC 9 cut(s) 713, 1033, 1201, 1690, 1898, 1906, 2296, 2719, 2948
Lsp1109I GCAGC 9 cut(s) 589, 709, 745, 820, 2286, 2449, 2672, 2936, 2939
LweI GCATC 9 cut(s) 109, 419, 442, 524, 770, 877, 1270, 1412, 2321
MaeI CTAG 6 cut(s) 426, 1106, 2006, 2073, 2847, 2870
MaeII ACGT 6 cut(s) 363, 1166, 1209, 1368, 1480, 2410
MaeIII GTNAC 3 cut(s) 1000, 1488, 2411
MfeI CAATTG 1 cut(s) 1124
MflI RGATCY 5 cut(s) 138, 178, 1153, 1454, 1939
MhlI GDGCHC 6 cut(s) 1038, 1644, 1808, 1903, 2388, 2536
MlsI TGGCCA 3 cut(s) 100, 321, 993
MluNI TGGCCA 3 cut(s) 100, 321, 993
MlyI GAGTC 3 cut(s) 14, 212, 2197
MmeI TCCRAC 3 cut(s) 315, 400, 805
Mox20I TGGCCA 3 cut(s) 100, 321, 993
MroXI GAANNNNTTC 4 cut(s) 786, 1076, 1482, 2217
MscI TGGCCA 3 cut(s) 100, 321, 993
MseI TTAA 8 cut(s) 135, 195, 294, 303, 543, 1116, 2372, 2478
MslI CAYNNNNRTG 2 cut(s) 486, 945
Msp20I TGGCCA 3 cut(s) 100, 321, 993
MspA1I CMGCKG 2 cut(s) 2663, 2930
MspCI CTTAAG 1 cut(s) 542
MspI CCGG 5 cut(s) 94, 1658, 1709, 1980, 2598
MspR9I CCNGG 2 cut(s) 995, 1710
MunI CAATTG 1 cut(s) 1124
Mva1269I GAATGC 3 cut(s) 891, 1726, 2674
MvaI CCWGG 1 cut(s) 995
MvnI CGCG 1 cut(s) 2169
NciI CCSGG 1 cut(s) 1710
NcoI CCATGG 1 cut(s) 104
NlaIV GGNNCC 8 cut(s) 75, 92, 909, 1155, 1350, 1950, 2501, 2992
NmeAIII GCCGAG 1 cut(s) 44
NmuCI GTSAC 1 cut(s) 1488
NspI RCATGY 2 cut(s) 1784, 2191
NspV TTCGAA 1 cut(s) 1973
PaeI GCATGC 1 cut(s) 2191
PagI TCATGA 1 cut(s) 853
PaqCI CACCTGC 2 cut(s) 383, 1846
PciSI GCTCTTC 1 cut(s) 2466
PcsI WCGNNNNNNNCGW 2 cut(s) 74, 2718
PctI GAATGC 3 cut(s) 891, 1726, 2674
PdmI GAANNNNTTC 4 cut(s) 786, 1076, 1482, 2217
PflFI GACNNNGTC 2 cut(s) 2726, 2882
PflMI CCANNNNNTGG 2 cut(s) 1283, 2771
PinAI ACCGGT 2 cut(s) 1657, 1979
PkrI GCNGC 9 cut(s) 604, 724, 760, 835, 2301, 2439, 2662, 2926, 2929
PleI GAGTC 3 cut(s) 13, 211, 2197
PpsI GAGTC 3 cut(s) 13, 211, 2197
PpuMI RGGWCCY 1 cut(s) 2500
PshAI GACNNNNGTC 1 cut(s) 1448
Psp124BI GAGCTC 1 cut(s) 1903
Psp5II RGGWCCY 1 cut(s) 2500
Psp6I CCWGG 1 cut(s) 993
PspGI CCWGG 1 cut(s) 993
PspN4I GGNNCC 8 cut(s) 75, 92, 909, 1155, 1350, 1950, 2501, 2992
PspPI GGNCC 6 cut(s) 74, 907, 1061, 1292, 2179, 2500
PspPPI RGGWCCY 1 cut(s) 2500
PstI CTGCAG 3 cut(s) 1162, 2295, 2439
PstNI CAGNNNCTG 4 cut(s) 206, 1283, 2299, 2930
PsuI RGATCY 5 cut(s) 138, 178, 1153, 1454, 1939
PsyI GACNNNGTC 2 cut(s) 2726, 2882
PvuII CAGCTG 2 cut(s) 2663, 2930
RsaI GTAC 6 cut(s) 243, 1136, 1433, 1779, 2565, 2697
RsaNI GTAC 6 cut(s) 242, 1135, 1432, 1778, 2564, 2696
RseI CAYNNNNRTG 2 cut(s) 486, 945
SacI GAGCTC 1 cut(s) 1903
SapI GCTCTTC 1 cut(s) 2466
SaqAI TTAA 8 cut(s) 135, 195, 294, 303, 543, 1116, 2372, 2478
SatI GCNGC 9 cut(s) 603, 723, 759, 834, 2300, 2438, 2661, 2925, 2928
Sau96I GGNCC 6 cut(s) 74, 907, 1061, 1292, 2179, 2500
SbfI CCTGCAGG 1 cut(s) 2295
ScaI AGTACT 1 cut(s) 1433
SchI GAGTC 3 cut(s) 14, 212, 2197
ScrFI CCNGG 2 cut(s) 995, 1710
SdaI CCTGCAGG 1 cut(s) 2295
SduI GDGCHC 6 cut(s) 1038, 1644, 1808, 1903, 2388, 2536
SfaNI GCATC 9 cut(s) 109, 419, 442, 524, 770, 877, 1270, 1412, 2321
SfcI CTRYAG 9 cut(s) 717, 1158, 1407, 1507, 1874, 2088, 2291, 2435, 2931
SfuI TTCGAA 1 cut(s) 1973
SinI GGWCC 4 cut(s) 74, 907, 1292, 2500
SmiMI CAYNNNNRTG 2 cut(s) 486, 945
SmlI CTYRAG 4 cut(s) 542, 1265, 2138, 2817
SmoI CTYRAG 4 cut(s) 542, 1265, 2138, 2817
SpeI ACTAGT 1 cut(s) 2072
SphI GCATGC 1 cut(s) 2191
Sse8387I CCTGCAGG 1 cut(s) 2295
SsiI CCGC 9 cut(s) 122, 308, 565, 1185, 1671, 2167, 2550, 2672, 2994
SspI AATATT 1 cut(s) 787
SspMI CTAG 6 cut(s) 426, 1106, 2006, 2073, 2847, 2870
SstI GAGCTC 1 cut(s) 1903
StyD4I CCNGG 2 cut(s) 993, 1708
StyI CCWWGG 4 cut(s) 104, 2005, 2495, 2654
TaaI ACNGT 7 cut(s) 1300, 1602, 1816, 2089, 2278, 2758, 2882
TaiI ACGT 6 cut(s) 366, 1169, 1212, 1371, 1483, 2413
TaqI TCGA 8 cut(s) 8, 210, 286, 510, 1973, 2198, 2556, 2721
TaqII GACCGA 2 cut(s) 91, 1438
TatI WGTACW 3 cut(s) 1431, 1777, 2563
Tru1I TTAA 8 cut(s) 135, 195, 294, 303, 543, 1116, 2372, 2478
Tru9I TTAA 8 cut(s) 135, 195, 294, 303, 543, 1116, 2372, 2478
TscAI CASTG 7 cut(s) 1194, 1607, 1753, 2281, 2536, 2761, 2772
TseFI GTSAC 1 cut(s) 1488
TseI GCWGC 9 cut(s) 602, 722, 758, 833, 2299, 2437, 2660, 2924, 2927
Tsp45I GTSAC 1 cut(s) 1488
TspGWI ACGGA 5 cut(s) 126, 131, 2024, 2131, 2697
TspRI CASTG 7 cut(s) 1194, 1607, 1753, 2281, 2536, 2761, 2772
Tth111I GACNNNGTC 2 cut(s) 2726, 2882
Van91I CCANNNNNTGG 2 cut(s) 1283, 2771
Vha464I CTTAAG 1 cut(s) 542
VneI GTGCAC 1 cut(s) 2384
VpaK11BI GGWCC 4 cut(s) 74, 907, 1292, 2500
XapI RAATTY 2 cut(s) 299, 2779
XceI RCATGY 2 cut(s) 1784, 2191
XcmI CCANNNNNNNNNTGG 1 cut(s) 821
XmaJI CCTAGG 1 cut(s) 2005
XmiI GTMKAC 1 cut(s) 1169
XmnI GAANNNNTTC 4 cut(s) 786, 1076, 1482, 2217
XspI CTAG 6 cut(s) 426, 1106, 2006, 2073, 2847, 2870
ZraI GACGTC 1 cut(s) 1167
ZrmI AGTACT 1 cut(s) 1433
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.