MD10G1153600.v1.1

Lipase (class 3)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0007769
Physical Location & Seq
Reverse (-)
24099328 .. 24104978
5651 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1153600.v1.1.491

Sequence Viewer

Length: 3105 bp
ATGGAGTCGATACAAAGCAAAGTGGAGGCATGGATCAAGGAGCAGCACGCCAAGCTCCTCAAGGTCTCTTGGTGCCCACTGCAGTGGAGGATGAAGTGGCCATTGCTTTTCAAGGAGGGCGACCGGGAGCACCGCCGCAGGATCCACCAGGAGTACGAGGGCCGCCGCAAGCAGCTCCACGATCTCTGCGCCGCCGTCAAGGTCGACTCCGTCTCTGACCTCCGGGAAATTCTCTGCTGCATGGTCCTCTCCGAGTGCGTTTACAAGAGACCTGCTTCGGACTTGGTTTGGGCTGTGAATAAGTTTAAGTCCTACTTCGGAGGACAAATTGTTTCTCTCGAGCGTGTTCAGCCCTCTTCGGATCACGTTCCGCACAGTTATTTGTTGGCGGAATCTGGTGATACATTGTTTGCTTCCTTCACTGGAACCAAGCAGTACAAGGATGTGATGACAGATGCAAACATATTTCAAGGTGCTATATTTCATGAGGATGTAGCGGAGGATACTAATGGGACTGAAACTACTAATCCACCTGAAAACCGAAAAGGAAATGGAGAACATTTGTGGAATCCACTTGAATCAAAAGCAAATAAAGCTAAACCTGCCGCACATCAGGGGTTCTTGGCTCGTGCTAAAGGGATACCTGCTTTGGAGCTGTACAGGCTTGCTCAAAAGAAAAACCGGAACCTTGTCTTATGTGGTCACTCACTTGGTGGAGCAGTAGCAGTGTTAGCTACCCTTTCTATTTTGAGGGTGGTTGCTGCTTCTTCTTCTTCATTAAAGGAGAATGGAAATGTTAAAGTCAAATGTATCACATTTTCCCAGCCCCCTGTTGGGAATGCAGCTTTAAGAGATTATGTTAGTAGAGAGGGATGGCAACATTACTTCAAGAGTTACTGCATTCCAGAAGATCTGGTGCCTCGCATCTTGTCTCCTGCTTATTTTCATCATTATAGTGCACAATCTTGTTCAGTGCCGGCTGAAACCGAAACTACCAGCATATCCATGTTAAAAAGTGAGGAAGCAGTAGGAAAAGGAAAAGAGAATGATGGCAAACGGTTGGTTCTGGGTTTGGGACCTGTTCAGACTTCTATGTGGAGACTCTCGAAGCTTGTTCCCTTAGAAGGCATAAGAAGACAATTCAACAAGTATAGAGGAAAGAAAGTTTATTCTGTCGAGGCATCTTCTCAGTCAGATTATGTAGCAGCAACTCTAGTAGATGATGACATGGTAGAACCACAGTCTCTTGAAATACAAGAAGGTTCTGATGGTATATCTCTTAAACCTATCTCTGACACTGATAAAGAGCCACCGTATGTGGTACCAAGTGGGAAGTCAGCTAGAAAAAGCTCCACAAAAAATGGGGATGACAGCACATGGCGCAGAGCTCCACAAAAAATGGGGATGACAGCACATGGCGCAGAGTGCCTTATTTACCTTCGTATGTACCATTCGGGGAGTGAGCTGTATGCTAAACTATATCTCTTGGAAAATTCATCAGTGAAGTCACTATCAGATGCGGAGTACTCAAATTTGACATCGGTCAGATCTGTTATTGCGGAACTGAGGGAACTATTTCAATCACATTCAATGAAGTCATATAGGTTTCGGTTCCAGAGAATCTATGACCTGTGCATGAAGGACGATACGTCACCTTTTCCAGCCATAGAGCAACTACAGCAGTTTCCACATCTACAGCAGTGGCTTGGTCTTGCAGTTGCAGGAACTGTGGAGCTTGGGCATATTGTTGAGTCTCCAGTTATTCGGACAGCTACTTCAGTTGCTCCTCTTGGATGGAATAGCATACCCGGCGAGAAGAATGGAGAGCCCTTAAAAGTTGATATCACTGGTTTTGGATTGCATCTTTGTACGCTTGTGCATGCCCAAGTGAATGGTAACTGGTGTTCAACAACAGTGGAATCCTTTCCTTCAACCCCAACTTATTCTTCAAATTATGGAGAAAAGCTGGAACTACAAAAAATGCGAGTCTTAGGTGGAGCTCCATTGAAACAGCCACCAAAATATCAGATGGAAGCAGATTCAATGATGCGTGTGTTTTCTTCTATTGATTCCAACACAGCTAATCTTAATAGGGATCACACTTCAGGACCATTTGATGAGGAAAAGTGGGAGCACACTTCAGGACCATTTGATGAGGAAAAGTCCATACTGACTTTACCAGTCTCTAAAGAGGTCCATGTGAGAACTCGCAGGGTGCGATTACTTGGTCTTGGAGCCGGAAAGACTTCCCTTTTTAAGGCAATGTTATCCCAAGGCAAAATTACTAATATTACCAATATTGAAAATCTGCTTCTAGAAACTAACGCCCAAGAAGGTATTTCTCGGGGTTTATACTTCTGTGATTCAGCAGGGGTAAATTTACAGGAGCTGAATTTGGAGGCCACCCGTTTCAGAGATGAACTATGGACAGGAATTCGTGATTTGAATCGGAAGACCGACTTGATTGTTCTTGTTCATAACCTGTCTCACAGAATACCAAGCTTAAATCTCTCAAATGGATCGCAGCAAAAGCCAGCCCTTACACTTCTGCTGGATGAAGCGAAATCTCTGGGAATTCCTTGGGTCCTTGCCGTAACAAACAAATTTTCTGTCAGTGCTCATCAGCAGAAAGCAGCAATTGATGATGTTGTCCAGTCATACCAAGCATCTCCAAGTACCACTGCCGTTATCAATTCCTGTCCATACGTCATGCCAAGTGCTGCAAGCACTAATTTGTCATGGGGTGCAACCACTGGAGATGCTGATGGAAGGATCAGTGCTCAAAGCTTATTGTTTGCTCCCATCAATTTTGTTAAGCGGCCTTTCCAGAAGAAAGAAATCATTCTCCCAGTGGAGGGTGTCAACTCTCTTCGTCAAGTAGTCCATCATGTGCTTCGTAGCCACGAGGAAGCTGCATTGCAGGAACTTGCAAGAGATGGGCTTTTGGTGGAAGTGGCACGAGAGCGTGGAATCGCTATGGGACGTGATTCTCAAGCCAAGTCAAATTCTCTTACATCTGCAGCCGTGGGTGCTTCCCTCGGAGCCGGTCTTGGCATTGTCTTGGCTGTTGTCATGGGTGCAGCATCCGCCTTGAGGAAGCCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_3

Protein Analysis

1035

Amino Acids

114.27

Weight (kDa)

8.55

Isoelectric Point (pI)

43.19

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_3 PF01764 186 - 309 6.1e-15 Lipase (class 3)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 3 cut(s) 280, 610, 652
Acc65I GGTACC 1 cut(s) 1321
AccB1I GGYRCC 3 cut(s) 72, 916, 1321
AccI GTMKAC 1 cut(s) 204
AclWI GGATC 7 cut(s) 41, 136, 149, 369, 2103, 2527, 2780
AcoI YGGCCR 1 cut(s) 98
AcsI RAATTY 9 cut(s) 228, 1492, 1531, 2377, 2392, 2433, 2574, 2603, 3004
AcuI CTGAAG 3 cut(s) 1761, 2088, 2124
AdeI CACNNNGTG 1 cut(s) 713
AfaI GTAC 8 cut(s) 155, 437, 659, 1323, 1448, 1526, 1870, 2677
AfiI CCNNNNNNNGG 7 cut(s) 833, 834, 1124, 2257, 2854, 2855, 3093
AhdI GACNNNNNGTC 1 cut(s) 1648
AjiI CACGTC 1 cut(s) 2984
AjnI CCWGG 1 cut(s) 147
AjuI GAANNNNNNNTTGG 2 cut(s) 1930, 1962
AleI CACNNNNGTG 1 cut(s) 82
Alw21I GWGCWC 7 cut(s) 132, 961, 1390, 2002, 2136, 2620, 2782
Alw26I GTCTC 9 cut(s) 70, 217, 262, 936, 1093, 1248, 1758, 2188, 2490
Alw44I GTGCAC 1 cut(s) 957
AlwI GGATC 7 cut(s) 41, 136, 149, 369, 2103, 2527, 2780
AlwNI CAGNNNCTG 2 cut(s) 1727, 2389
Ama87I CYCGRG 2 cut(s) 338, 2343
AoxI GGCC 4 cut(s) 98, 160, 2400, 2819
ApaLI GTGCAC 1 cut(s) 957
ApoI RAATTY 9 cut(s) 228, 1492, 1531, 2377, 2392, 2433, 2574, 2603, 3004
ArsI GACNNNNNNTTYG 2 cut(s) 2595, 2627
Asp700I GAANNNNTTC 4 cut(s) 1575, 1923, 2245, 2841
Asp718I GGTACC 1 cut(s) 1321
AspLEI GCGC 3 cut(s) 191, 1383, 1421
AspS9I GGNCC 7 cut(s) 160, 244, 1076, 2108, 2144, 2194, 2584
AsuC2I CCSGG 3 cut(s) 125, 224, 1809
AsuHPI GGTGA 2 cut(s) 410, 1644
AvaI CYCGRG 2 cut(s) 338, 2343
AvaII GGWCC 6 cut(s) 244, 1076, 2108, 2144, 2194, 2584
BaeGI GKGCMC 2 cut(s) 77, 961
BalI TGGCCA 1 cut(s) 100
BamHI GGATCC 1 cut(s) 141
BanI GGYRCC 3 cut(s) 72, 916, 1321
BanII GRGCYC 3 cut(s) 1390, 1830, 2002
BauI CACGAG 3 cut(s) 627, 2903, 2958
BbsI GAAGAC 2 cut(s) 1141, 2459
Bbv12I GWGCWC 7 cut(s) 132, 961, 1390, 2002, 2136, 2620, 2782
BccI CCATC 9 cut(s) 867, 1043, 1262, 1788, 2023, 2759, 2810, 2892, 2930
BceAI ACGGC 4 cut(s) 179, 2576, 2669, 3008
BcgI CGANNNNNNTGC 2 cut(s) 2894, 2928
BciT130I CCWGG 1 cut(s) 149
BciVI GTATCC 2 cut(s) 496, 633
BcnI CCSGG 3 cut(s) 125, 224, 1809
BcoDI GTCTC 9 cut(s) 70, 217, 262, 936, 1093, 1248, 1758, 2188, 2490
BfaI CTAG 3 cut(s) 1214, 1341, 2315
BfmI CTRYAG 4 cut(s) 80, 1676, 1694, 3018
BfuAI ACCTGC 3 cut(s) 280, 610, 652
BfuI GTATCC 2 cut(s) 496, 633
BglII AGATCT 2 cut(s) 910, 1547
BmcAI AGTACT 1 cut(s) 1526
Bme1390I CCNGG 4 cut(s) 125, 149, 224, 1809
Bme18I GGWCC 6 cut(s) 244, 1076, 2108, 2144, 2194, 2584
BmeRI GACNNNNNGTC 1 cut(s) 1648
BmeT110I CYCGRG 2 cut(s) 338, 2343
BmgBI CACGTC 1 cut(s) 2984
BmgT120I GGNCC 7 cut(s) 160, 244, 1076, 2108, 2144, 2194, 2584
BmrFI CCNGG 4 cut(s) 125, 149, 224, 1809
BmrI ACTGGG 1 cut(s) 2843
BmsI GCATC 9 cut(s) 445, 933, 1190, 1507, 1870, 2037, 2675, 2749, 3092
BmuI ACTGGG 1 cut(s) 2843
BoxI GACNNNNGTC 1 cut(s) 1541
BpiI GAAGAC 2 cut(s) 1141, 2459
BpmI CTGGAG 2 cut(s) 1740, 2775
BpuEI CTTGAG 2 cut(s) 44, 2976
BpuMI CCSGG 3 cut(s) 125, 224, 1809
BsaBI GATNNNNATC 1 cut(s) 2445
BsaI GGTCTC 2 cut(s) 70, 262
BsaJI CCNNGG 4 cut(s) 2272, 2579, 3024, 3037
BsaWI WCCGGW 1 cut(s) 681
Bsc4I CCNNNNNNNGG 7 cut(s) 833, 834, 1124, 2257, 2854, 2855, 3093
Bse118I RCCGGY 2 cut(s) 976, 3044
Bse1I ACTGG 8 cut(s) 427, 1757, 1852, 1904, 2180, 2653, 2758, 2849
Bse3DI GCAATG 3 cut(s) 101, 2268, 2915
Bse8I GATNNNNATC 1 cut(s) 2445
BseBI CCWGG 1 cut(s) 149
BseDI CCNNGG 4 cut(s) 2272, 2579, 3024, 3037
BseGI GGATG 9 cut(s) 96, 448, 496, 878, 1372, 1410, 1799, 2560, 3083
BseJI GATNNNNATC 1 cut(s) 2445
BseLI CCNNNNNNNGG 7 cut(s) 833, 834, 1124, 2257, 2854, 2855, 3093
BseMI GCAATG 3 cut(s) 101, 2268, 2915
BseMII CTCAG 2 cut(s) 1202, 1556
BseNI ACTGG 8 cut(s) 427, 1757, 1852, 1904, 2180, 2653, 2758, 2849
BseRI GAGGAG 2 cut(s) 47, 1776
BseSI GKGCMC 2 cut(s) 77, 961
BseYI CCCAGC 1 cut(s) 822
BsgI GTGCAG 1 cut(s) 3099
Bsh1285I CGRYCG 1 cut(s) 124
BshFI GGCC 4 cut(s) 100, 162, 2402, 2821
BshNI GGYRCC 3 cut(s) 72, 916, 1321
BsiEI CGRYCG 1 cut(s) 124
BsiHKAI GWGCWC 7 cut(s) 132, 961, 1390, 2002, 2136, 2620, 2782
BsiHKCI CYCGRG 2 cut(s) 338, 2343
BsiSI CCGG 7 cut(s) 124, 223, 682, 977, 1809, 2238, 3045
BslFI GGGAC 3 cut(s) 526, 1089, 2994
BslI CCNNNNNNNGG 7 cut(s) 833, 834, 1124, 2257, 2854, 2855, 3093
BsmAI GTCTC 9 cut(s) 70, 217, 262, 936, 1093, 1248, 1758, 2188, 2490
BsmBI CGTCTC 1 cut(s) 217
BsmFI GGGAC 3 cut(s) 526, 1089, 2994
BsmI GAATGC 2 cut(s) 844, 900
BsnI GGCC 4 cut(s) 100, 162, 2402, 2821
Bso31I GGTCTC 2 cut(s) 70, 262
BsoBI CYCGRG 2 cut(s) 338, 2343
Bsp1286I GDGCHC 9 cut(s) 77, 132, 961, 1390, 1830, 2002, 2136, 2620, 2782
Bsp1407I TGTACA 1 cut(s) 657
Bsp143I GATC 9 cut(s) 33, 141, 181, 361, 910, 1547, 2095, 2519, 2772
BspANI GGCC 4 cut(s) 100, 162, 2402, 2821
BspCNI CTCAG 2 cut(s) 1201, 1557
BspHI TCATGA 1 cut(s) 484
BspMAI CTGCAG 2 cut(s) 84, 3022
BspMI ACCTGC 3 cut(s) 280, 610, 652
BspPI GGATC 7 cut(s) 41, 136, 149, 369, 2103, 2527, 2780
BspT107I GGYRCC 3 cut(s) 72, 916, 1321
BspTNI GGTCTC 2 cut(s) 70, 262
BsrDI GCAATG 3 cut(s) 101, 2268, 2915
BsrFI RCCGGY 2 cut(s) 976, 3044
BsrGI TGTACA 1 cut(s) 657
BsrI ACTGG 8 cut(s) 427, 1757, 1852, 1904, 2180, 2653, 2758, 2849
BssAI RCCGGY 2 cut(s) 976, 3044
BssECI CCNNGG 4 cut(s) 2272, 2579, 3024, 3037
BssMI GATC 9 cut(s) 33, 141, 181, 361, 910, 1547, 2095, 2519, 2772
BssSI CACGAG 3 cut(s) 627, 2903, 2958
BssT1I CCWWGG 2 cut(s) 2272, 2579
Bst2BI CACGAG 3 cut(s) 627, 2903, 2958
Bst2UI CCWGG 1 cut(s) 149
Bst4CI ACNGT 6 cut(s) 377, 1059, 1242, 1314, 1729, 1915
Bst6I CTCTTC 2 cut(s) 361, 2874
BstAUI TGTACA 1 cut(s) 657
BstC8I GCNNGC 7 cut(s) 48, 170, 666, 978, 1881, 2535, 2725
BstDEI CTNAG 5 cut(s) 1120, 1188, 1565, 1990, 3102
BstDSI CCRYGG 1 cut(s) 3024
BstENI CCTNNNNNAGG 1 cut(s) 2255
BstF5I GGATG 9 cut(s) 96, 448, 496, 878, 1372, 1410, 1799, 2560, 3083
BstHHI GCGC 3 cut(s) 191, 1383, 1421
BstKTI GATC 9 cut(s) 36, 144, 184, 364, 913, 1550, 2098, 2522, 2775
BstMAI GTCTC 9 cut(s) 70, 217, 262, 936, 1093, 1248, 1758, 2188, 2490
BstMBI GATC 9 cut(s) 33, 141, 181, 361, 910, 1547, 2095, 2519, 2772
BstMCI CGRYCG 1 cut(s) 124
BstNI CCWGG 1 cut(s) 149
BstNSI RCATGY 1 cut(s) 1883
BstPAI GACNNNNGTC 1 cut(s) 1541
BstSCI CCNGG 4 cut(s) 123, 147, 222, 1807
BstSFI CTRYAG 4 cut(s) 80, 1676, 1694, 3018
BstSLI GKGCMC 2 cut(s) 77, 961
BstV2I GAAGAC 2 cut(s) 1141, 2459
BstX2I RGATCY 3 cut(s) 141, 910, 1547
BstXI CCANNNNNNTGG 2 cut(s) 84, 1892
BstYI RGATCY 3 cut(s) 141, 910, 1547
BsuI GTATCC 2 cut(s) 496, 633
BsuRI GGCC 4 cut(s) 100, 162, 2402, 2821
BtgI CCRYGG 1 cut(s) 3024
BtrI CACGTC 1 cut(s) 2984
BtsCI GGATG 9 cut(s) 96, 448, 496, 878, 1372, 1410, 1799, 2560, 3083
BtsI GCAGTG 5 cut(s) 77, 89, 732, 1706, 2679
BveI ACCTGC 3 cut(s) 280, 610, 652
Cac8I GCNNGC 7 cut(s) 48, 170, 666, 978, 1881, 2535, 2725
CaiI CAGNNNCTG 2 cut(s) 1727, 2389
CciI TCATGA 1 cut(s) 484
CfoI GCGC 3 cut(s) 191, 1383, 1421
Cfr10I RCCGGY 2 cut(s) 976, 3044
Cfr13I GGNCC 7 cut(s) 160, 244, 1076, 2108, 2144, 2194, 2584
Csp6I GTAC 8 cut(s) 154, 436, 658, 1322, 1447, 1525, 1869, 2676
CspCI CAANNNNNGTGG 2 cut(s) 1896, 1931
CviQI GTAC 8 cut(s) 154, 436, 658, 1322, 1447, 1525, 1869, 2676
DdeI CTNAG 5 cut(s) 1120, 1188, 1565, 1990, 3102
DpnI GATC 9 cut(s) 35, 143, 183, 363, 912, 1549, 2097, 2521, 2774
DpnII GATC 9 cut(s) 33, 141, 181, 361, 910, 1547, 2095, 2519, 2772
DraIII CACNNNGTG 1 cut(s) 713
DriI GACNNNNNGTC 1 cut(s) 1648
EaeI YGGCCR 1 cut(s) 98
Eam1104I CTCTTC 2 cut(s) 361, 2874
Eam1105I GACNNNNNGTC 1 cut(s) 1648
EarI CTCTTC 2 cut(s) 361, 2874
EciI GGCGGA 2 cut(s) 404, 3076
Ecl136II GAGCTC 2 cut(s) 1388, 2000
Eco130I CCWWGG 2 cut(s) 2272, 2579
Eco24I GRGCYC 3 cut(s) 1390, 1830, 2002
Eco31I GGTCTC 2 cut(s) 70, 262
Eco32I GATATC 1 cut(s) 1843
Eco47I GGWCC 6 cut(s) 244, 1076, 2108, 2144, 2194, 2584
Eco53kI GAGCTC 2 cut(s) 1388, 2000
Eco57I CTGAAG 3 cut(s) 1761, 2088, 2124
Eco88I CYCGRG 2 cut(s) 338, 2343
EcoICRI GAGCTC 2 cut(s) 1388, 2000
EcoNI CCTNNNNNAGG 1 cut(s) 2255
EcoO109I RGGNCCY 2 cut(s) 1076, 2584
EcoRI GAATTC 2 cut(s) 2433, 2574
EcoRII CCWGG 1 cut(s) 147
EcoRV GATATC 1 cut(s) 1843
EcoT14I CCWWGG 2 cut(s) 2272, 2579
EcoT38I GRGCYC 3 cut(s) 1390, 1830, 2002
ErhI CCWWGG 2 cut(s) 2272, 2579
Esp3I CGTCTC 1 cut(s) 217
FalI AAGNNNNNCTT 4 cut(s) 299, 331, 2444, 2476
FaqI GGGAC 3 cut(s) 526, 1089, 2994
FblI GTMKAC 1 cut(s) 204
FokI GGATG 9 cut(s) 103, 455, 503, 885, 1379, 1417, 1806, 2567, 3070
FriOI GRGCYC 3 cut(s) 1390, 1830, 2002
FspBI CTAG 3 cut(s) 1214, 1341, 2315
GlaI GCGC 3 cut(s) 190, 1382, 1420
GsaI CCCAGC 1 cut(s) 826
GsuI CTGGAG 2 cut(s) 1740, 2775
HaeIII GGCC 4 cut(s) 100, 162, 2402, 2821
HapII CCGG 7 cut(s) 124, 223, 682, 977, 1809, 2238, 3045
HhaI GCGC 3 cut(s) 191, 1383, 1421
Hin6I GCGC 3 cut(s) 189, 1381, 1419
HinP1I GCGC 3 cut(s) 189, 1381, 1419
HincII GTYRAC 2 cut(s) 205, 2863
HindII GTYRAC 2 cut(s) 205, 2863
HindIII AAGCTT 3 cut(s) 1109, 2500, 2785
HpaII CCGG 7 cut(s) 124, 223, 682, 977, 1809, 2238, 3045
HphI GGTGA 2 cut(s) 410, 1644
Hpy166II GTNNAC 4 cut(s) 205, 262, 959, 2863
Hpy8I GTNNAC 4 cut(s) 205, 262, 959, 2863
HpyAV CCTTC 8 cut(s) 427, 1118, 1253, 1448, 1633, 1938, 2327, 2763
HpyCH4III ACNGT 6 cut(s) 377, 1059, 1242, 1314, 1729, 1915
HpyCH4IV ACGT 4 cut(s) 366, 1649, 2706, 2983
HpyF3I CTNAG 5 cut(s) 1120, 1188, 1565, 1990, 3102
HpySE526I ACGT 4 cut(s) 366, 1649, 2706, 2983
HspAI GCGC 3 cut(s) 189, 1381, 1419
KpnI GGTACC 1 cut(s) 1325
KroI GCCGGC 1 cut(s) 976
KroNI GCCGGC 1 cut(s) 978
Kzo9I GATC 9 cut(s) 33, 141, 181, 361, 910, 1547, 2095, 2519, 2772
LweI GCATC 9 cut(s) 445, 933, 1190, 1507, 1870, 2037, 2675, 2749, 3092
MaeI CTAG 3 cut(s) 1214, 1341, 2315
MaeII ACGT 4 cut(s) 366, 1649, 2706, 2983
MaeIII GTNAC 6 cut(s) 701, 893, 1506, 1650, 1895, 2593
MalI GATC 9 cut(s) 35, 143, 183, 363, 912, 1549, 2097, 2521, 2774
MboI GATC 9 cut(s) 33, 141, 181, 361, 910, 1547, 2095, 2519, 2772
MfeI CAATTG 1 cut(s) 2637
MflI RGATCY 3 cut(s) 141, 910, 1547
MhlI GDGCHC 9 cut(s) 77, 132, 961, 1390, 1830, 2002, 2136, 2620, 2782
MlsI TGGCCA 1 cut(s) 100
MluNI TGGCCA 1 cut(s) 100
MlyI GAGTC 5 cut(s) 14, 200, 1095, 1760, 1995
MmeI TCCRAC 1 cut(s) 2097
Mox20I TGGCCA 1 cut(s) 100
MroNI GCCGGC 1 cut(s) 976
MroXI GAANNNNTTC 4 cut(s) 1575, 1923, 2245, 2841
MscI TGGCCA 1 cut(s) 100
MslI CAYNNNNRTG 4 cut(s) 82, 489, 954, 1004
Msp20I TGGCCA 1 cut(s) 100
MspI CCGG 7 cut(s) 124, 223, 682, 977, 1809, 2238, 3045
MspR9I CCNGG 4 cut(s) 125, 149, 224, 1809
MunI CAATTG 1 cut(s) 2637
Mva1269I GAATGC 2 cut(s) 844, 900
MvaI CCWGG 1 cut(s) 149
NaeI GCCGGC 1 cut(s) 978
NciI CCSGG 3 cut(s) 125, 224, 1809
NdeII GATC 9 cut(s) 33, 141, 181, 361, 910, 1547, 2095, 2519, 2772
NgoMIV GCCGGC 1 cut(s) 976
NmuCI GTSAC 3 cut(s) 701, 1506, 1650
NspI RCATGY 1 cut(s) 1883
OliI CACNNNNGTG 1 cut(s) 82
PaeI GCATGC 1 cut(s) 1883
PaeR7I CTCGAG 1 cut(s) 338
PagI TCATGA 1 cut(s) 484
PcsI WCGNNNNNNNCGW 1 cut(s) 2215
PctI GAATGC 2 cut(s) 844, 900
PdiI GCCGGC 1 cut(s) 978
PdmI GAANNNNTTC 4 cut(s) 1575, 1923, 2245, 2841
PflFI GACNNNGTC 1 cut(s) 209
PfoI TCCNGGA 1 cut(s) 222
PleI GAGTC 5 cut(s) 13, 200, 1095, 1759, 1994
PpsI GAGTC 5 cut(s) 13, 200, 1095, 1759, 1994
PpuMI RGGWCCY 2 cut(s) 1076, 2584
PshAI GACNNNNGTC 1 cut(s) 1541
Psp124BI GAGCTC 2 cut(s) 1390, 2002
Psp5II RGGWCCY 2 cut(s) 1076, 2584
Psp6I CCWGG 1 cut(s) 147
PspFI CCCAGC 1 cut(s) 822
PspGI CCWGG 1 cut(s) 147
PspPI GGNCC 7 cut(s) 160, 244, 1076, 2108, 2144, 2194, 2584
PspPPI RGGWCCY 2 cut(s) 1076, 2584
PsrI GAACNNNNNNTAC 2 cut(s) 1888, 1920
PstI CTGCAG 2 cut(s) 84, 3022
PstNI CAGNNNCTG 2 cut(s) 1727, 2389
PsuI RGATCY 3 cut(s) 141, 910, 1547
PsyI GACNNNGTC 1 cut(s) 209
RsaI GTAC 8 cut(s) 155, 437, 659, 1323, 1448, 1526, 1870, 2677
RsaNI GTAC 8 cut(s) 154, 436, 658, 1322, 1447, 1525, 1869, 2676
RseI CAYNNNNRTG 4 cut(s) 82, 489, 954, 1004
SacI GAGCTC 2 cut(s) 1390, 2002
SalI GTCGAC 1 cut(s) 203
Sau3AI GATC 9 cut(s) 33, 141, 181, 361, 910, 1547, 2095, 2519, 2772
Sau96I GGNCC 7 cut(s) 160, 244, 1076, 2108, 2144, 2194, 2584
ScaI AGTACT 1 cut(s) 1526
SchI GAGTC 5 cut(s) 14, 200, 1095, 1760, 1995
ScrFI CCNGG 4 cut(s) 125, 149, 224, 1809
SduI GDGCHC 9 cut(s) 77, 132, 961, 1390, 1830, 2002, 2136, 2620, 2782
SfaNI GCATC 9 cut(s) 445, 933, 1190, 1507, 1870, 2037, 2675, 2749, 3092
SfcI CTRYAG 4 cut(s) 80, 1676, 1694, 3018
Sfr274I CTCGAG 1 cut(s) 338
SinI GGWCC 6 cut(s) 244, 1076, 2108, 2144, 2194, 2584
SlaI CTCGAG 1 cut(s) 338
SmiMI CAYNNNNRTG 4 cut(s) 82, 489, 954, 1004
SmlI CTYRAG 4 cut(s) 59, 338, 2991, 3091
SmoI CTYRAG 4 cut(s) 59, 338, 2991, 3091
SphI GCATGC 1 cut(s) 1883
SspI AATATT 2 cut(s) 2290, 2299
SspMI CTAG 3 cut(s) 1214, 1341, 2315
SstI GAGCTC 2 cut(s) 1390, 2002
StyD4I CCNGG 4 cut(s) 123, 147, 222, 1807
StyI CCWWGG 2 cut(s) 2272, 2579
TaaI ACNGT 6 cut(s) 377, 1059, 1242, 1314, 1729, 1915
TaiI ACGT 4 cut(s) 369, 1652, 2709, 2986
TaqI TCGA 5 cut(s) 8, 204, 339, 1106, 1176
TaqII GACCGA 2 cut(s) 1531, 2471
TatI WGTACW 3 cut(s) 435, 657, 1524
TauI GCSGC 6 cut(s) 138, 165, 168, 194, 608, 2821
TseFI GTSAC 3 cut(s) 701, 1506, 1650
Tsp45I GTSAC 3 cut(s) 701, 1506, 1650
TspGWI ACGGA 1 cut(s) 199
Tth111I GACNNNGTC 1 cut(s) 209
VneI GTGCAC 1 cut(s) 957
VpaK11BI GGWCC 6 cut(s) 244, 1076, 2108, 2144, 2194, 2584
XagI CCTNNNNNAGG 1 cut(s) 2255
XapI RAATTY 9 cut(s) 228, 1492, 1531, 2377, 2392, 2433, 2574, 2603, 3004
XbaI TCTAGA 1 cut(s) 2314
XceI RCATGY 1 cut(s) 1883
XcmI CCANNNNNNNNNTGG 1 cut(s) 830
XhoI CTCGAG 1 cut(s) 338
XmiI GTMKAC 1 cut(s) 204
XmnI GAANNNNTTC 4 cut(s) 1575, 1923, 2245, 2841
XspI CTAG 3 cut(s) 1214, 1341, 2315
ZrmI AGTACT 1 cut(s) 1526
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.