Rw6G023000

Lipase (class 3)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr6
OG0007769
Physical Location & Seq
Reverse (-)
45195423 .. 45200980
5558 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw6G023000.1

Sequence Viewer

Length: 3063 bp
ATGGAATCGCTACAAAGCCGAGTGGAGTCATGGCTGAGGGATTGGGAGAAGCGAGCCAAGCTCTGGAAGGTGTCCTGGGGCCCACTCCAGTGGAGGATGAGGTGGCCTTGGATTCTCAACGACGGCGACAGGGAGCAGCGGAAGAGGATCCACGAGGAGTACGAGCGTCGCCGGAAGCAGCTCCACGATCTCTGCGCCGCCGTCAAGGCTGACTCCGTCTCCGACTTGCAGGACATTTTCTGCTGCATGGTGCTCTCAGAGTGCGTGTATAAGAGACCTGCTTCGGACTTGGTTCGCGCGGTGAACAAATTCAAGGCTGACTTTGGAGGACAGATTGTTGCGCTGGAGCGTGTGCAGCCTTCTTCTGATCACGTTCCTCACAGTTACTTGTTGGCGGAAGCAGGTGATACATTGTTTGCTTCCTTCATTGGAACTAAGCAGTACAAGGATGTGATGACCGATGCAAACATACTGCAAGGTGCTATATTTCATGAGGATCCTGTGGAGGATACTGATGGGACTAGTCCACCTGGAAGTTGCAAGGGAAATGGGGAGAATTCGTTTAATCCTCTTGAATCAAAAACGAAGCCAGTAAATAATAAAGCTAAACCTGCTGCTCATCGGGGGTTCTTGGCTCGTGCTAAAGGTATACCTGCTCTGGAGTTGTACAGGCTTGCTCAAAAGAAAAAACGAAACCTTGTTTTATGTGGACACTCACTTGGTGGAGCAGTAGCAGTATTAGCTACCCTTGCTATTTTGAGGGTTGTTGCTGCTTCTTCTTCATCTAAGGAGAACGGAACTGTTCGAGTCAAATGTATTACATTTTCCCAGCCCCCTGTTGGCAATGCCGCTTTAAGAGATTATGTTAATAGAGAGGGGTGGGAGCATTACTTCAAAAGTTACTGCATTCCAGAAGATCTGGTGCCCCGCATCTTGTCTCCTGCCTATTTTCACCATTATAATGCACAACCTCTTTCAATGCCTGCTGGAAATGAAACTACTAAGAAATCCATGTTAAGAAGTGAGGAAGCAGTAGGAAAACGTAAAGTGAATGATGGGGAGCAATTGGTTCTGGGTTTGGGACCTGTACAGAGTTCTATGTGGAGACTTTCAAGACTTGTTCCTTTAGAAGGTGTAAGAAGACAGTTTAATAAATACAGAGGAAGGAAAGTTGCATCTGTCGAAACATCTTCTCAGTCGGATTCGGTTGCAACAGCTATAGTAGATGATGACATAGTTGAACCAGAGTCTCTAGAAATCCAAGAGGGTTCTGATGGTATATCTCTTAAGCCCATCTCTGACATTGATAAAGACATGCCAGATGTGGCGTCAAATGGAAACTTAGCTAGTAAAAGCACCACTAGTAGCGGGGATGACAGGAGATGGCGTAGAGTGCCCTCTTTACCTTCATACATACCATTTGGAGAGCTTTATCTCTTGGAAGATTCATCAGTGAAGTCACTATCAGATGCAGAGTACTCAAAGTTGACGACATCGGTCAAATCTGTGATTGCGGAACTGAGGGAAAGATTTCAGTCACATTCAATGAAATCATATAGGTCTCGATTCCAGAGAATCTATGACCTTTGCATGAGAGATGATACCTCACTTTTTTCGGGAATAGAGCAACAACAGTTTCCGCATCTACAACAATGGCTTGGTCTTGCAGTTGCAGGAAATGTGGAGCTTGGGCATATTGTTGAGTCTCCAGTTATTCGGACAGCTACTTCAGTTGCTCCTCTTGGATGGAATGGCATACCTGGTGAGAAAAATGGAGATCCACTAAAAGTGGATATCACTGGTTTTGGATTGCATCTTTGTACGCTTGTTCACGCCCAAGTGAATGGTAACTGGTGTTCAACTACTGTAGAATCCTTTCCTTCAACCCCAACTTACTCTTCAGATTATGGAGAGAAGCCAGAACTACAAAAAATGCGAGTGTTAATTGGAGCTCCTTTGAGACAGCCACCTAAGCATCAAATGGTAGCAGATTCTTTGTTGAATGTGTTTCCTTCTATTGATCCCAACACAACTACTCTTAATAGGGAGCACATATCAGGACCATTTATTGAAGAAAAGTCCATCCGTCCTGAAGGTTTAAGCGACTTTTCCATATTTTGTACCAGTGATTTTACCACAGTTTCTAAAGAGGTTCATGTGAGAACCCGTAGGGTGCGGTTACTTGGCCTTGAGGGTGCCGGTAAGACTTCTCTTTTCAAGGCAATATTATCTCAAGGCAGGATAACCAACATTCCCAACATTGAAAATCTGCTTCCAGAGGCTGATGTCCAAGAAGGTATTTCTGGTGGCTTGTGCTTCTGCGATTCAGCAGGGATAAATTTGCAGGAGCTGAATTTGGAGTCCACTCGTCTCAGAGATGAACTCTGGACAGGAATTCGTGATCTTAGTCGAAAGACAGACTTGATTGTTCTTGTTCATAACCTGTCTCATAGAATACCTCGCTGTAATTACTCAAATGGATCACAGCAAAAGCCGGCCATTTCACTTCTATTGGATGAGGCAAAATCTGTTGGAATTCCTTGGGTTCTTGCTATTACAAATAAATTTTCTGTCAGTGCTCATCAACAGAAAGCATCGATTGATGCTGTTGTCCAGTCCTATCAAGCATCTCCAAATACCACTGGAGTTATCAATTCCTGTCCGTATGTGATGCCCAGTGCTGCAAGTATATGGGGTGCAAGCGTTGAAGATGCCGATGGGAGGTCAGGTGCACAAAAGCTTCTCTTTGCTCCCATCAATTTTGTTAAAAGGCCTTTTCAGAAGAAAGAAATTATACTCCCAGTCGAGGGTGTCAATTCTCTTCGTCAAATAGTTCATCACGTGCTTCGTAGCCGTGAGGAAGAATCCTTACAGGAACTTGCGAGAGACAGGCTTTTGGTTGAATTATCACGAGATCGTGCATTGGCTATGAATGCAAGTCGTGATTCTAAAGCCAAGGAAAATTCACTTTCAGCTGCAACTGTAGGAGCTTCCCTTGGAGCTGGTCTTGGCATTGTTTTAGCTGTTGTCATGGGAGCAGCGTCTGCCTTGAGGAAGCCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_3

Protein Analysis

1020

Amino Acids

112.74

Weight (kDa)

8.57

Isoelectric Point (pI)

49.81

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_3 PF01764 196 - 311 5e-15 Lipase (class 3)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 960
AarI CACCTGC 1 cut(s) 392
Acc36I ACCTGC 4 cut(s) 286, 392, 619, 661
AccB1I GGYRCC 2 cut(s) 922, 2195
AccB7I CCANNNNNTGG 1 cut(s) 63
AccI GTMKAC 1 cut(s) 649
AccII CGCG 2 cut(s) 297, 299
AclWI GGATC 7 cut(s) 142, 155, 491, 504, 1772, 2015, 2488
AcoI YGGCCR 1 cut(s) 2496
AcsI RAATTY 8 cut(s) 308, 556, 2338, 2353, 2394, 2535, 2564, 2962
AcuI CTGAAG 3 cut(s) 1713, 1884, 2112
AcvI CACGTG 1 cut(s) 2842
AcyI GRCGYC 1 cut(s) 1328
AdeI CACNNNGTG 1 cut(s) 722
AfaI GTAC 7 cut(s) 161, 443, 668, 1089, 1478, 1822, 2122
AfiI CCNNNNNNNGG 6 cut(s) 63, 839, 1130, 2721, 2806, 2807
AflII CTTAAG 1 cut(s) 1286
AhlI ACTAGT 2 cut(s) 521, 1361
AjnI CCWGG 3 cut(s) 74, 529, 1759
AjuI GAANNNNNNNTTGG 2 cut(s) 1882, 1914
AleI CACNNNNGTG 1 cut(s) 88
Alw21I GWGCWC 5 cut(s) 255, 1954, 2052, 2581, 2734
Alw44I GTGCAC 1 cut(s) 2730
AlwI GGATC 7 cut(s) 142, 155, 491, 504, 1772, 2015, 2488
AlwNI CAGNNNCTG 3 cut(s) 2282, 2350, 3044
AoxI GGCC 5 cut(s) 79, 104, 2185, 2496, 2771
ApaI GGGCCC 1 cut(s) 83
ApaLI GTGCAC 1 cut(s) 2730
ApeKI GCWGC 9 cut(s) 136, 178, 243, 355, 614, 770, 2681, 2975, 3038
ApoI RAATTY 8 cut(s) 308, 556, 2338, 2353, 2394, 2535, 2564, 2962
Asp700I GAANNNNTTC 3 cut(s) 308, 1530, 1875
AspLEI GCGC 3 cut(s) 197, 299, 343
AspS9I GGNCC 4 cut(s) 79, 80, 1082, 2060
AsuHPI GGTGA 4 cut(s) 313, 416, 944, 1775
AvaII GGWCC 2 cut(s) 1082, 2060
BaeGI GKGCMC 4 cut(s) 83, 927, 1398, 2734
BamHI GGATCC 2 cut(s) 147, 496
BanI GGYRCC 2 cut(s) 922, 2195
BanII GRGCYC 2 cut(s) 83, 1954
BarI GAAGNNNNNNTAC 2 cut(s) 2853, 2885
BauI CACGAG 3 cut(s) 152, 636, 2910
BbrPI CACGTG 1 cut(s) 2842
BbsI GAAGAC 1 cut(s) 1147
Bbv12I GWGCWC 5 cut(s) 255, 1954, 2052, 2581, 2734
BbvCI CCTCAGC 1 cut(s) 35
BbvI GCAGC 9 cut(s) 148, 190, 230, 367, 601, 757, 2668, 2962, 3050
BccI CCATC 9 cut(s) 509, 1049, 1268, 1301, 1377, 1740, 2090, 2711, 2762
BceAI ACGGC 3 cut(s) 139, 185, 2838
BciT130I CCWGG 3 cut(s) 76, 531, 1761
BciVI GTATCC 1 cut(s) 502
BclI TGATCA 1 cut(s) 367
BcuI ACTAGT 2 cut(s) 521, 1361
BfaI CTAG 4 cut(s) 522, 1253, 1347, 1362
BfmI CTRYAG 3 cut(s) 1218, 1866, 2982
BfrI CTTAAG 1 cut(s) 1286
BfuAI ACCTGC 4 cut(s) 286, 392, 619, 661
BfuI GTATCC 1 cut(s) 502
BglI GCCNNNNNGGC 1 cut(s) 206
BglII AGATCT 1 cut(s) 916
BmcAI AGTACT 1 cut(s) 1478
Bme1390I CCNGG 3 cut(s) 76, 531, 1761
Bme18I GGWCC 2 cut(s) 1082, 2060
BmgT120I GGNCC 4 cut(s) 79, 80, 1082, 2060
BmiI GGNNCC 7 cut(s) 80, 81, 149, 498, 924, 1083, 2197
BmrFI CCNGG 3 cut(s) 76, 531, 1761
BmrI ACTGGG 2 cut(s) 2670, 2795
BmuI ACTGGG 2 cut(s) 2670, 2795
BoxI GACNNNNGTC 1 cut(s) 1496
BpiI GAAGAC 1 cut(s) 1147
BplI GAGNNNNNCTC 4 cut(s) 45, 77, 2367, 2399
BpmI CTGGAG 5 cut(s) 71, 365, 680, 1692, 2664
Bpu10I CCTNAGC 2 cut(s) 35, 1971
BpuEI CTTGAG 2 cut(s) 2210, 2217
Bsa29I ATCGAT 1 cut(s) 2597
BsaAI YACGTR 1 cut(s) 2842
BsaHI GRCGYC 1 cut(s) 1328
BsaI GGTCTC 2 cut(s) 268, 1566
BsaJI CCNNGG 5 cut(s) 75, 107, 2540, 2955, 2995
BsaXI ACNNNNNCTCC 8 cut(s) 203, 233, 545, 575, 1372, 1402, 2713, 2743
Bsc4I CCNNNNNNNGG 6 cut(s) 63, 839, 1130, 2721, 2806, 2807
Bse118I RCCGGY 2 cut(s) 2198, 2494
Bse3DI GCAATG 1 cut(s) 850
BseBI CCWGG 3 cut(s) 76, 531, 1761
BseCI ATCGAT 1 cut(s) 2597
BseDI CCNNGG 5 cut(s) 75, 107, 2540, 2955, 2995
BseGI GGATG 6 cut(s) 102, 454, 1378, 1751, 2082, 2521
BseLI CCNNNNNNNGG 6 cut(s) 63, 839, 1130, 2721, 2806, 2807
BseMI GCAATG 1 cut(s) 850
BseMII CTCAG 5 cut(s) 26, 270, 1208, 1511, 2386
BseRI GAGGAG 2 cut(s) 170, 1728
BseSI GKGCMC 4 cut(s) 83, 927, 1398, 2734
BseXI GCAGC 9 cut(s) 148, 190, 230, 367, 601, 757, 2668, 2962, 3050
BseYI CCCAGC 1 cut(s) 828
BsgI GTGCAG 1 cut(s) 374
Bsh1236I CGCG 2 cut(s) 297, 299
BshFI GGCC 5 cut(s) 81, 106, 2187, 2498, 2773
BshNI GGYRCC 2 cut(s) 922, 2195
BshVI ATCGAT 1 cut(s) 2597
BsiHKAI GWGCWC 5 cut(s) 255, 1954, 2052, 2581, 2734
BsiSI CCGG 3 cut(s) 172, 2199, 2495
BslFI GGGAC 2 cut(s) 532, 1095
BslI CCNNNNNNNGG 6 cut(s) 63, 839, 1130, 2721, 2806, 2807
BsmBI CGTCTC 2 cut(s) 223, 2375
BsmFI GGGAC 2 cut(s) 532, 1095
BsmI GAATGC 2 cut(s) 906, 2938
BsnI GGCC 5 cut(s) 81, 106, 2187, 2498, 2773
Bso31I GGTCTC 2 cut(s) 268, 1566
Bsp120I GGGCCC 1 cut(s) 79
Bsp1286I GDGCHC 8 cut(s) 83, 255, 927, 1398, 1954, 2052, 2581, 2734
Bsp1407I TGTACA 2 cut(s) 666, 1087
BspANI GGCC 5 cut(s) 81, 106, 2187, 2498, 2773
BspCNI CTCAG 5 cut(s) 27, 269, 1207, 1512, 2385
BspDI ATCGAT 1 cut(s) 2597
BspFNI CGCG 2 cut(s) 297, 299
BspHI TCATGA 1 cut(s) 490
BspLI GGNNCC 7 cut(s) 80, 81, 149, 498, 924, 1083, 2197
BspMI ACCTGC 4 cut(s) 286, 392, 619, 661
BspPI GGATC 7 cut(s) 142, 155, 491, 504, 1772, 2015, 2488
BspT107I GGYRCC 2 cut(s) 922, 2195
BspTI CTTAAG 1 cut(s) 1286
BspTNI GGTCTC 2 cut(s) 268, 1566
BsrDI GCAATG 1 cut(s) 850
BsrFI RCCGGY 2 cut(s) 2198, 2494
BsrGI TGTACA 2 cut(s) 666, 1087
BssAI RCCGGY 2 cut(s) 2198, 2494
BssECI CCNNGG 5 cut(s) 75, 107, 2540, 2955, 2995
BssNAI GTATAC 1 cut(s) 650
BssNI GRCGYC 1 cut(s) 1328
BssSI CACGAG 3 cut(s) 152, 636, 2910
BssT1I CCWWGG 4 cut(s) 107, 2540, 2955, 2995
Bst1107I GTATAC 1 cut(s) 650
Bst2BI CACGAG 3 cut(s) 152, 636, 2910
Bst2UI CCWGG 3 cut(s) 76, 531, 1761
Bst4CI ACNGT 7 cut(s) 383, 802, 1146, 1635, 1867, 2140, 2983
Bst6I CTCTTC 3 cut(s) 137, 1903, 2826
BstACI GRCGYC 1 cut(s) 1328
BstAFI CTTAAG 1 cut(s) 1286
BstAPI GCANNNNNTGC 1 cut(s) 3044
BstAUI TGTACA 2 cut(s) 666, 1087
BstBAI YACGTR 1 cut(s) 2842
BstC8I GCNNGC 5 cut(s) 54, 675, 984, 2496, 2701
BstENI CCTNNNNNAGG 1 cut(s) 1128
BstF5I GGATG 6 cut(s) 102, 454, 1378, 1751, 2082, 2521
BstFNI CGCG 2 cut(s) 297, 299
BstHHI GCGC 3 cut(s) 197, 299, 343
BstNI CCWGG 3 cut(s) 76, 531, 1761
BstNSI RCATGY 1 cut(s) 1318
BstPAI GACNNNNGTC 1 cut(s) 1496
BstSCI CCNGG 3 cut(s) 74, 529, 1759
BstSFI CTRYAG 3 cut(s) 1218, 1866, 2982
BstSLI GKGCMC 4 cut(s) 83, 927, 1398, 2734
BstUI CGCG 2 cut(s) 297, 299
BstV1I GCAGC 9 cut(s) 148, 190, 230, 367, 601, 757, 2668, 2962, 3050
BstV2I GAAGAC 1 cut(s) 1147
BstX2I RGATCY 4 cut(s) 147, 496, 916, 1777
BstXI CCANNNNNNTGG 2 cut(s) 90, 1844
BstYI RGATCY 4 cut(s) 147, 496, 916, 1777
BstZ17I GTATAC 1 cut(s) 650
Bsu15I ATCGAT 1 cut(s) 2597
BsuI GTATCC 1 cut(s) 502
BsuRI GGCC 5 cut(s) 81, 106, 2187, 2498, 2773
BsuTUI ATCGAT 1 cut(s) 2597
BtsCI GGATG 6 cut(s) 102, 454, 1378, 1751, 2082, 2521
BtsIMutI CAGTG 7 cut(s) 95, 1458, 1797, 2131, 2581, 2640, 2683
BveI ACCTGC 4 cut(s) 286, 392, 619, 661
Cac8I GCNNGC 5 cut(s) 54, 675, 984, 2496, 2701
CaiI CAGNNNCTG 3 cut(s) 2282, 2350, 3044
CciI TCATGA 1 cut(s) 490
CfoI GCGC 3 cut(s) 197, 299, 343
Cfr10I RCCGGY 2 cut(s) 2198, 2494
Cfr13I GGNCC 4 cut(s) 79, 80, 1082, 2060
ClaI ATCGAT 1 cut(s) 2597
CseI GACGC 3 cut(s) 155, 1317, 3030
CsiI ACCWGGT 1 cut(s) 1759
Csp6I GTAC 7 cut(s) 160, 442, 667, 1088, 1477, 1821, 2121
CviAII CATG 8 cut(s) 30, 247, 491, 1012, 1315, 1591, 2156, 3031
CviQI GTAC 7 cut(s) 160, 442, 667, 1088, 1477, 1821, 2121
DraIII CACNNNGTG 1 cut(s) 722
EaeI YGGCCR 1 cut(s) 2496
Eam1104I CTCTTC 3 cut(s) 137, 1903, 2826
EarI CTCTTC 3 cut(s) 137, 1903, 2826
EciI GGCGGA 1 cut(s) 410
Ecl136II GAGCTC 1 cut(s) 1952
Eco130I CCWWGG 4 cut(s) 107, 2540, 2955, 2995
Eco147I AGGCCT 1 cut(s) 2773
Eco24I GRGCYC 2 cut(s) 83, 1954
Eco31I GGTCTC 2 cut(s) 268, 1566
Eco32I GATATC 1 cut(s) 1795
Eco47I GGWCC 2 cut(s) 1082, 2060
Eco53kI GAGCTC 1 cut(s) 1952
Eco57I CTGAAG 3 cut(s) 1713, 1884, 2112
Eco72I CACGTG 1 cut(s) 2842
EcoICRI GAGCTC 1 cut(s) 1952
EcoNI CCTNNNNNAGG 1 cut(s) 1128
EcoO109I RGGNCCY 2 cut(s) 79, 1082
EcoRI GAATTC 3 cut(s) 556, 2394, 2535
EcoRII CCWGG 3 cut(s) 74, 529, 1759
EcoRV GATATC 1 cut(s) 1795
EcoT14I CCWWGG 4 cut(s) 107, 2540, 2955, 2995
EcoT38I GRGCYC 2 cut(s) 83, 1954
ErhI CCWWGG 4 cut(s) 107, 2540, 2955, 2995
Esp3I CGTCTC 2 cut(s) 223, 2375
FaeI CATG 8 cut(s) 33, 250, 494, 1015, 1318, 1594, 2159, 3034
FalI AAGNNNNNCTT 6 cut(s) 305, 337, 2405, 2437, 2729, 2761
FaqI GGGAC 2 cut(s) 532, 1095
FatI CATG 8 cut(s) 29, 246, 490, 1011, 1314, 1590, 2155, 3030
FauI CCCGC 2 cut(s) 935, 1361
FbaI TGATCA 1 cut(s) 367
FblI GTMKAC 1 cut(s) 649
FokI GGATG 6 cut(s) 109, 461, 1385, 1758, 2069, 2528
FriOI GRGCYC 2 cut(s) 83, 1954
FspBI CTAG 4 cut(s) 522, 1253, 1347, 1362
GlaI GCGC 3 cut(s) 196, 298, 342
GsaI CCCAGC 1 cut(s) 832
GsuI CTGGAG 5 cut(s) 71, 365, 680, 1692, 2664
HaeIII GGCC 5 cut(s) 81, 106, 2187, 2498, 2773
HapII CCGG 3 cut(s) 172, 2199, 2495
HgaI GACGC 3 cut(s) 155, 1317, 3030
HhaI GCGC 3 cut(s) 197, 299, 343
Hin1I GRCGYC 1 cut(s) 1328
Hin1II CATG 8 cut(s) 33, 250, 494, 1015, 1318, 1594, 2159, 3034
Hin6I GCGC 3 cut(s) 195, 297, 341
HinP1I GCGC 3 cut(s) 195, 297, 341
HincII GTYRAC 1 cut(s) 1488
HindII GTYRAC 1 cut(s) 1488
HindIII AAGCTT 1 cut(s) 2738
HpaII CCGG 3 cut(s) 172, 2199, 2495
HphI GGTGA 4 cut(s) 313, 416, 944, 1775
Hpy166II GTNNAC 8 cut(s) 304, 527, 650, 710, 1488, 1831, 2364, 2732
Hpy8I GTNNAC 8 cut(s) 304, 527, 650, 710, 1488, 1831, 2364, 2732
Hpy99I CGWCG 2 cut(s) 125, 171
HpyCH4III ACNGT 7 cut(s) 383, 802, 1146, 1635, 1867, 2140, 2983
HpyCH4IV ACGT 3 cut(s) 372, 1042, 2841
HpySE526I ACGT 3 cut(s) 372, 1042, 2841
Hsp92I GRCGYC 1 cut(s) 1328
Hsp92II CATG 8 cut(s) 33, 250, 494, 1015, 1318, 1594, 2159, 3034
HspAI GCGC 3 cut(s) 195, 297, 341
KroI GCCGGC 1 cut(s) 2494
KroNI GCCGGC 1 cut(s) 2496
Ksp22I TGATCA 1 cut(s) 367
Lsp1109I GCAGC 9 cut(s) 148, 190, 230, 367, 601, 757, 2668, 2962, 3050
MabI ACCWGGT 1 cut(s) 1759
MaeI CTAG 4 cut(s) 522, 1253, 1347, 1362
MaeII ACGT 3 cut(s) 372, 1042, 2841
MaeIII GTNAC 6 cut(s) 383, 899, 1458, 1536, 1847, 2178
MfeI CAATTG 1 cut(s) 1064
MflI RGATCY 4 cut(s) 147, 496, 916, 1777
MhlI GDGCHC 8 cut(s) 83, 255, 927, 1398, 1954, 2052, 2581, 2734
MlyI GAGTC 6 cut(s) 35, 206, 816, 1256, 1712, 2369
MmeI TCCRAC 3 cut(s) 246, 1179, 2512
MroNI GCCGGC 1 cut(s) 2494
MroXI GAANNNNTTC 3 cut(s) 308, 1530, 1875
MslI CAYNNNNRTG 2 cut(s) 88, 960
MspA1I CMGCKG 2 cut(s) 139, 2975
MspCI CTTAAG 1 cut(s) 1286
MspI CCGG 3 cut(s) 172, 2199, 2495
MspR9I CCNGG 3 cut(s) 76, 531, 1761
MunI CAATTG 1 cut(s) 1064
Mva1269I GAATGC 2 cut(s) 906, 2938
MvaI CCWGG 3 cut(s) 76, 531, 1761
MvnI CGCG 2 cut(s) 297, 299
NaeI GCCGGC 1 cut(s) 2496
NgoMIV GCCGGC 1 cut(s) 2494
NlaIII CATG 8 cut(s) 33, 250, 494, 1015, 1318, 1594, 2159, 3034
NlaIV GGNNCC 7 cut(s) 80, 81, 149, 498, 924, 1083, 2197
NmeAIII GCCGAG 1 cut(s) 44
NmuCI GTSAC 2 cut(s) 1458, 1536
NspI RCATGY 1 cut(s) 1318
OliI CACNNNNGTG 1 cut(s) 88
PagI TCATGA 1 cut(s) 490
PaqCI CACCTGC 1 cut(s) 392
PceI AGGCCT 1 cut(s) 2773
PcsI WCGNNNNNNNCGW 1 cut(s) 159
PctI GAATGC 2 cut(s) 906, 2938
PdiI GCCGGC 1 cut(s) 2496
PdmI GAANNNNTTC 3 cut(s) 308, 1530, 1875
PflFI GACNNNGTC 1 cut(s) 215
PflMI CCANNNNNTGG 1 cut(s) 63
PleI GAGTC 6 cut(s) 34, 206, 815, 1255, 1711, 2368
PmaCI CACGTG 1 cut(s) 2842
PmlI CACGTG 1 cut(s) 2842
PpsI GAGTC 6 cut(s) 34, 206, 815, 1255, 1711, 2368
Ppu21I YACGTR 1 cut(s) 2842
PpuMI RGGWCCY 1 cut(s) 1082
PshAI GACNNNNGTC 1 cut(s) 1496
PsiI TTATAA 1 cut(s) 960
Psp124BI GAGCTC 1 cut(s) 1954
Psp5II RGGWCCY 1 cut(s) 1082
Psp6I CCWGG 3 cut(s) 74, 529, 1759
PspCI CACGTG 1 cut(s) 2842
PspFI CCCAGC 1 cut(s) 828
PspGI CCWGG 3 cut(s) 74, 529, 1759
PspN4I GGNNCC 7 cut(s) 80, 81, 149, 498, 924, 1083, 2197
PspOMI GGGCCC 1 cut(s) 79
PspPI GGNCC 4 cut(s) 79, 80, 1082, 2060
PspPPI RGGWCCY 1 cut(s) 1082
PsrI GAACNNNNNNTAC 2 cut(s) 1840, 1872
PstNI CAGNNNCTG 3 cut(s) 2282, 2350, 3044
PsuI RGATCY 4 cut(s) 147, 496, 916, 1777
PsyI GACNNNGTC 1 cut(s) 215
PvuII CAGCTG 1 cut(s) 2975
RsaI GTAC 7 cut(s) 161, 443, 668, 1089, 1478, 1822, 2122
RsaNI GTAC 7 cut(s) 160, 442, 667, 1088, 1477, 1821, 2121
RseI CAYNNNNRTG 2 cut(s) 88, 960
SacI GAGCTC 1 cut(s) 1954
Sau96I GGNCC 4 cut(s) 79, 80, 1082, 2060
ScaI AGTACT 1 cut(s) 1478
SchI GAGTC 6 cut(s) 35, 206, 816, 1256, 1712, 2369
ScrFI CCNGG 3 cut(s) 76, 531, 1761
SduI GDGCHC 8 cut(s) 83, 255, 927, 1398, 1954, 2052, 2581, 2734
SexAI ACCWGGT 1 cut(s) 1759
SfcI CTRYAG 3 cut(s) 1218, 1866, 2982
SinI GGWCC 2 cut(s) 1082, 2060
SmiMI CAYNNNNRTG 2 cut(s) 88, 960
SmlI CTYRAG 4 cut(s) 1286, 2189, 2232, 3049
SmoI CTYRAG 4 cut(s) 1286, 2189, 2232, 3049
SpeI ACTAGT 2 cut(s) 521, 1361
SseBI AGGCCT 1 cut(s) 2773
SspI AATATT 1 cut(s) 2226
SspMI CTAG 4 cut(s) 522, 1253, 1347, 1362
SstI GAGCTC 1 cut(s) 1954
StuI AGGCCT 1 cut(s) 2773
StyD4I CCNGG 3 cut(s) 74, 529, 1759
StyI CCWWGG 4 cut(s) 107, 2540, 2955, 2995
TaaI ACNGT 7 cut(s) 383, 802, 1146, 1635, 1867, 2140, 2983
TaiI ACGT 3 cut(s) 375, 1045, 2844
TaqI TCGA 6 cut(s) 805, 1182, 1564, 2410, 2597, 2805
TaqII GACCGA 2 cut(s) 473, 1486
TatI WGTACW 4 cut(s) 441, 666, 1087, 1476
TauI GCSGC 2 cut(s) 200, 851
TscAI CASTG 7 cut(s) 95, 1458, 1804, 2131, 2581, 2647, 2683
TseFI GTSAC 2 cut(s) 1458, 1536
TseI GCWGC 9 cut(s) 136, 178, 243, 355, 614, 770, 2681, 2975, 3038
Tsp45I GTSAC 2 cut(s) 1458, 1536
TspGWI ACGGA 4 cut(s) 205, 810, 2075, 2652
TspRI CASTG 7 cut(s) 95, 1458, 1804, 2131, 2581, 2647, 2683
Tth111I GACNNNGTC 1 cut(s) 215
Van91I CCANNNNNTGG 1 cut(s) 63
Vha464I CTTAAG 1 cut(s) 1286
VneI GTGCAC 1 cut(s) 2730
VpaK11BI GGWCC 2 cut(s) 1082, 2060
XagI CCTNNNNNAGG 1 cut(s) 1128
XapI RAATTY 8 cut(s) 308, 556, 2338, 2353, 2394, 2535, 2564, 2962
XbaI TCTAGA 1 cut(s) 1252
XceI RCATGY 1 cut(s) 1318
XcmI CCANNNNNNNNNTGG 1 cut(s) 836
XmiI GTMKAC 1 cut(s) 649
XmnI GAANNNNTTC 3 cut(s) 308, 1530, 1875
XspI CTAG 4 cut(s) 522, 1253, 1347, 1362
ZrmI AGTACT 1 cut(s) 1478
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.