MD05G1163900.v1.1

Lipase (class 3)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0007769
Physical Location & Seq
Reverse (-)
29285549 .. 29291472
5924 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1163900.v1.1.491

Sequence Viewer

Length: 3066 bp
ATGGAGTCGATACAAAGCAGAGTGGAGGCATGGATCAAGGAGCAGCGAGCCAAGCTCCTCAAGGTCTCCTGGGGTCCTCTGCAGTGGAGGATGAAGTGGCCCTGGCTCGGCAAGGAGGGCGGCCGCGAGCACCGCCGCAGGATCCACCAGGAGTACGAGCGCCGCCGCAAGCAGCTCCACGATCTCTGCGCCGCCGTCAAGGCCGACTCCGTCTCCGACCTCCAGGACATCCTCTGCTGCATGGTTCTCTCCGAGTGCGTTTACAAGAGACCTGCTTCGGACTTGGTTTGGGCTGTGAATAAGTTTAAGTCCGACTTCGGAGGACAAATTGTTTCTCTCGAGCGTGTTCAACCTTCTTCGGATCACGTTCCTCACAGTTATTTGTTGGCGGAATCCGGTGATACATTGTTTGCTTCTTTCATTGGAACCAAGCAGTACAAGGATGTGATGGCGGATGCAAACATATTTCAAGGTGCTATATTTCATGAGGATGTAGTGGAGGATACTAATGGGACTGAAACTACTAAATGTAATCCACCTGAAAAAGGAAAGGGAAATGGAGAAAATTTGTGGAATCCTCTTGAATCAAAAGCAAATAAAGCTAAACCTGCCGCACATCGGGGGTTCTTGGCTCGTGCCAAAGGGATACCTGCGTTGGAGTTGTACAGGCTTGCTCAAAAGAAAAAACGGAACCTTGTGTTATGTGGTCACTCACTTGGTGGAGCAGTAGCAGTATTAGCTACCCTTGCTATTTTGAGGGTGGTCGCTTCTTCTTCTTCTTCATTAAAGGAGAATGGAAATGTTAAAGTCAAATGCATCACATTTTCCCAGCCCCCTGTTGGGAATGCAGCTTTAAGAGATTATGTTAGTAGAGAGGGATGGCAGCATTACTTCAAGAGTTACTGCATTCCTGAAGATCTGGTGCCTCGCATCCTGTCTCCTGCTTATTTTCATCATTATAACGCACAATCTCCTTCAGTGCCTGCTGAAACCGGAACTACCAGGATATCCATGTTAAAAAGCGAGGAAGTAGTAGGACAACGCAAAGAGAATGGTGGCGAGCAGTTGGTTCTGGGTTTGGGACCTGTGCAGACTTCTATGTGGAGACTTTCGAAGCTTGTTCCCTTAGAAGGCATAAGAAGACAATTTAACAAATACAGAGGAAAGAAAGTTGATTCTGTGGAGACATCTTCTCAATCAGATTCTGTTGCAACAACTGTAGTAGATGATGACATGGTAGAACCACAGTCTCTTGAAATACAAGAAGGTTCTGATGGTATATCTCTTAAGCCTATCTCAGAAACTGGTAAAGAGCCACCGGGTGTAGCACCAAGTGGGAAGTTAGCTAAAAAAAGCTCCACAAAAAATGGGGATAGCCGCACATGGCGCAGAGTCCCTTATTTACCTTCATATGTACCATTTGGGGAGCTTTATCTCTTGGACAATTCGTCAGTGAAGTCACTATCAGATGCAGAGTACTCAAAGTTGACATCGGTCAGGTCTGTGATTGCGGAGCTGAGGGAACGATTTCAATCACATTCAATGAAGTCATATAGGTTTCGCTTCCAGAGAATCTATGACCTGTGCATGAAAGATGATACGTCACCTTTTTCAGCCATAGAACAACTACAGCAGTTTCCACATCTACAGCAGTGGCTTGGTCTTGCAGTTGCAGGAACTGTGGAGCTTGGGCATATTGTTGAGTCTCCAGTTATTCGGACAGCTACTTCAGTTGCTCCTCTTGGGTGGAATGGCATACCTGGTGAGAAGAACGGAGATCCCGTAAAAGTTGATATCACTGGTTATGGACTGCATCTTTGTACACTTGTGCATGCCCAAGTGAATGGTAACTGGTGTTCAACAACAGTAGAATCGTTTCCTTCAACCCCAGCTTATTCTTCAAATTATGGAGAAAATCTGGAACTACAAAAAATGCGAGTCTTAATTGGAGCCCCATTGAAACAGCCACCAAAGCATCAGATGGAAGCAGATTCAATGATGCATGTGTATTCTGCTATTGATTCCAGCACAGCTAATCTTAATAGGGAGCACACTTCAGGACCATTTCATGAGGAAAAGTCCATGCGTCCTGAAGGGTTAAGTGAATTTTTCATATTTTGTACCAGTGACTTCACCACAGTTTCTAAAGAGGTGCATGTGAGAACTCGTAGGGTGCGGTTACTCGGTCTTGAGGGAGCCGGAAAGACTTCCCTTTTTAAGGCAATACTATCCCAAGGCAGAATTACCCATATTACAAATATTGAGAATCTGCTTCCAGAAACTGACGCCCAAGAAGGTATTTCTCTTGGTGTATGCTTCTGTGATTCAGCAGGGGTAAATTTGCAGGAGCTGAATTTGGAGGCTACTCGTTTCAGAGATGAACTATGGACAGGAATTCGTGATCTTAATCGGAAGACCGACTTGATTGTTCTTGTTCATAACTTGTCTCATAGAATACCACACTTCAATCACTCAAATGGATCGCAACAAAAGCCAGCCCTTACACTTCTGCTGGACGAGGCGAAATCTTTGGGAATTCCTTGGGTCCTTGCCGTAACAAACAAATTTTCTGTAAGTGCTCATCAGCAGAAGGCAGCAATTGATGCTGTTGTCCAGTCATACCAAGCATCTCCAAGGACCACGGGCGTTATCAATTCCTGTCCATATGTCACGCCAAGTGCAGCAAGCACTAATTTGTCATGGGGTGCAACCACCGGAGATGCTGATGGAAGGATGGGTGCTCAAAGCTTATTTTTTGCTCCCATCAATTTTGTTAAGCGGCCTTTCCAGAAGAAAGAAATTATTCTCCCAGTTGAGGGTGTCAACTCTCTTCGTCAAGTAGTCCATCATGTACTGCGGAGCCATGAGGAAGCTGCGTTGCAGGAACTTGCAAGAGATAGGCTGTTGGTGGAAGTGGCAAGAGAGCGTGCAATCGCCATGGGACGTGATTCTCAAGCCAAGTCAAATTCTCTTACTTCTGCAGCTGTGGGTGCTTCCCTCGGAGCCGGTCTTGGCATTGTCTTGGCTGTTGTCATGGGTGCAGCATCCGCCTTGAGGAAGCCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_3

Protein Analysis

1022

Amino Acids

112.66

Weight (kDa)

8.91

Isoelectric Point (pI)

44.33

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_3 PF01764 196 - 311 8.1e-15 Lipase (class 3)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 960
Acc36I ACCTGC 3 cut(s) 280, 616, 658
AccB1I GGYRCC 1 cut(s) 922
AccII CGCG 1 cut(s) 126
AclWI GGATC 6 cut(s) 41, 136, 149, 369, 1772, 2488
AcoI YGGCCR 1 cut(s) 121
AcsI RAATTY 8 cut(s) 565, 2105, 2338, 2353, 2394, 2535, 2564, 2965
AcuI CTGAAG 5 cut(s) 933, 960, 1713, 2040, 2112
AcyI GRCGYC 1 cut(s) 2286
AdeI CACNNNGTG 3 cut(s) 719, 1322, 1334
AfaI GTAC 8 cut(s) 155, 437, 665, 1416, 1478, 1822, 2122, 2853
AflII CTTAAG 1 cut(s) 1286
AhdI GACNNNNNGTC 1 cut(s) 1447
AjiI CACGTC 1 cut(s) 2945
AjnI CCWGG 6 cut(s) 68, 101, 147, 222, 1001, 1759
Alw21I GWGCWC 4 cut(s) 132, 2052, 2581, 2743
Alw26I GTCTC 9 cut(s) 70, 217, 262, 942, 1099, 1178, 1254, 1710, 2451
AlwI GGATC 6 cut(s) 41, 136, 149, 369, 1772, 2488
AlwNI CAGNNNCTG 6 cut(s) 983, 1205, 1304, 1679, 2282, 2350
Ama87I CYCGRG 1 cut(s) 338
AoxI GGCC 4 cut(s) 98, 121, 201, 2780
ApoI RAATTY 8 cut(s) 565, 2105, 2338, 2353, 2394, 2535, 2564, 2965
ArsI GACNNNNNNTTYG 2 cut(s) 1800, 1832
Asp700I GAANNNNTTC 4 cut(s) 1527, 1875, 2206, 2802
AspLEI GCGC 3 cut(s) 162, 191, 1389
AspS9I GGNCC 6 cut(s) 74, 99, 1082, 2060, 2545, 2637
AsuC2I CCSGG 1 cut(s) 1320
AsuHPI GGTGA 4 cut(s) 410, 1596, 1775, 2125
AsuII TTCGAA 1 cut(s) 1112
AvaI CYCGRG 1 cut(s) 338
AvaII GGWCC 5 cut(s) 74, 1082, 2060, 2545, 2637
BaeI ACNNNNGTAYC 2 cut(s) 638, 671
BamHI GGATCC 1 cut(s) 141
BanI GGYRCC 1 cut(s) 922
BanII GRGCYC 1 cut(s) 1954
BauI CACGAG 1 cut(s) 633
BbsI GAAGAC 2 cut(s) 1147, 2420
Bbv12I GWGCWC 4 cut(s) 132, 2052, 2581, 2743
BbvCI CCTCAGC 1 cut(s) 1517
BccI CCATC 8 cut(s) 442, 873, 1268, 1975, 2720, 2728, 2771, 2853
BceAI ACGGC 2 cut(s) 179, 2537
BciT130I CCWGG 6 cut(s) 70, 103, 149, 224, 1003, 1761
BciVI GTATCC 2 cut(s) 496, 639
BcnI CCSGG 1 cut(s) 1320
BcoDI GTCTC 9 cut(s) 70, 217, 262, 942, 1099, 1178, 1254, 1710, 2451
BfmI CTRYAG 5 cut(s) 80, 1218, 1628, 1646, 2979
BfoI RGCGCY 1 cut(s) 163
BfrI CTTAAG 1 cut(s) 1286
BfuAI ACCTGC 3 cut(s) 280, 616, 658
BfuI GTATCC 2 cut(s) 496, 639
BglI GCCNNNNNGGC 1 cut(s) 200
BglII AGATCT 1 cut(s) 916
BmcAI AGTACT 1 cut(s) 1478
Bme1390I CCNGG 7 cut(s) 70, 103, 149, 224, 1003, 1320, 1761
Bme18I GGWCC 5 cut(s) 74, 1082, 2060, 2545, 2637
BmeRI GACNNNNNGTC 1 cut(s) 1447
BmeT110I CYCGRG 1 cut(s) 338
BmgBI CACGTC 1 cut(s) 2945
BmgT120I GGNCC 6 cut(s) 74, 99, 1082, 2060, 2545, 2637
BmrFI CCNGG 7 cut(s) 70, 103, 149, 224, 1003, 1320, 1761
BmrI ACTGGG 1 cut(s) 2804
BmuI ACTGGG 1 cut(s) 2804
BoxI GACNNNNGTC 1 cut(s) 1493
BpiI GAAGAC 2 cut(s) 1147, 2420
BplI GAGNNNNNCTC 2 cut(s) 39, 71
BpmI CTGGAG 2 cut(s) 206, 1692
Bpu10I CCTNAGC 1 cut(s) 1517
Bpu14I TTCGAA 1 cut(s) 1112
BpuEI CTTGAG 3 cut(s) 44, 2210, 2937
BpuMI CCSGG 1 cut(s) 1320
BsaBI GATNNNNATC 2 cut(s) 1531, 2406
BsaHI GRCGYC 1 cut(s) 2286
BsaI GGTCTC 2 cut(s) 70, 262
BsaJI CCNNGG 8 cut(s) 69, 101, 2233, 2540, 2633, 2640, 2937, 2998
BsaWI WCCGGW 3 cut(s) 395, 992, 2714
BsaXI ACNNNNNCTCC 4 cut(s) 197, 227, 1767, 1797
Bse118I RCCGGY 1 cut(s) 3005
Bse1I ACTGG 7 cut(s) 1309, 1709, 1804, 1856, 2124, 2614, 2810
Bse8I GATNNNNATC 2 cut(s) 1531, 2406
BseBI CCWGG 6 cut(s) 70, 103, 149, 224, 1003, 1761
BseDI CCNNGG 8 cut(s) 69, 101, 2233, 2540, 2633, 2640, 2937, 2998
BseGI GGATG 9 cut(s) 96, 228, 448, 460, 496, 884, 930, 2739, 3044
BseJI GATNNNNATC 2 cut(s) 1531, 2406
BseMII CTCAG 2 cut(s) 1311, 1508
BseNI ACTGG 7 cut(s) 1309, 1709, 1804, 1856, 2124, 2614, 2810
BseRI GAGGAG 2 cut(s) 47, 1728
BseX3I CGGCCG 1 cut(s) 121
BseYI CCCAGC 2 cut(s) 828, 1888
BsgI GTGCAG 3 cut(s) 1109, 2700, 3060
Bsh1236I CGCG 1 cut(s) 126
Bsh1285I CGRYCG 1 cut(s) 124
BshFI GGCC 4 cut(s) 100, 123, 203, 2782
BshNI GGYRCC 1 cut(s) 922
BsiEI CGRYCG 1 cut(s) 124
BsiHKAI GWGCWC 4 cut(s) 132, 2052, 2581, 2743
BsiHKCI CYCGRG 1 cut(s) 338
BsiSI CCGG 6 cut(s) 396, 993, 1319, 2199, 2715, 3006
BslFI GGGAC 4 cut(s) 526, 1095, 1379, 2955
BsmAI GTCTC 9 cut(s) 70, 217, 262, 942, 1099, 1178, 1254, 1710, 2451
BsmBI CGTCTC 1 cut(s) 217
BsmFI GGGAC 4 cut(s) 526, 1095, 1379, 2955
BsmI GAATGC 2 cut(s) 850, 906
BsnI GGCC 4 cut(s) 100, 123, 203, 2782
Bso31I GGTCTC 2 cut(s) 70, 262
BsoBI CYCGRG 1 cut(s) 338
Bsp119I TTCGAA 1 cut(s) 1112
Bsp1286I GDGCHC 5 cut(s) 132, 1954, 2052, 2581, 2743
Bsp1407I TGTACA 2 cut(s) 663, 1820
Bsp143I GATC 8 cut(s) 33, 141, 181, 361, 916, 1777, 2401, 2480
Bsp19I CCATGG 1 cut(s) 2937
BspANI GGCC 4 cut(s) 100, 123, 203, 2782
BspCNI CTCAG 2 cut(s) 1310, 1509
BspFNI CGCG 1 cut(s) 126
BspHI TCATGA 2 cut(s) 484, 2068
BspMAI CTGCAG 2 cut(s) 84, 2983
BspMI ACCTGC 3 cut(s) 280, 616, 658
BspPI GGATC 6 cut(s) 41, 136, 149, 369, 1772, 2488
BspT104I TTCGAA 1 cut(s) 1112
BspT107I GGYRCC 1 cut(s) 922
BspTI CTTAAG 1 cut(s) 1286
BspTNI GGTCTC 2 cut(s) 70, 262
BsrFI RCCGGY 1 cut(s) 3005
BsrGI TGTACA 2 cut(s) 663, 1820
BsrI ACTGG 7 cut(s) 1309, 1709, 1804, 1856, 2124, 2614, 2810
BssAI RCCGGY 1 cut(s) 3005
BssECI CCNNGG 8 cut(s) 69, 101, 2233, 2540, 2633, 2640, 2937, 2998
BssMI GATC 8 cut(s) 33, 141, 181, 361, 916, 1777, 2401, 2480
BssNI GRCGYC 1 cut(s) 2286
BssSI CACGAG 1 cut(s) 633
BssT1I CCWWGG 4 cut(s) 2233, 2540, 2633, 2937
Bst2BI CACGAG 1 cut(s) 633
Bst2UI CCWGG 6 cut(s) 70, 103, 149, 224, 1003, 1761
Bst4CI ACNGT 6 cut(s) 377, 1219, 1248, 1681, 1867, 2140
Bst6I CTCTTC 1 cut(s) 2835
BstACI GRCGYC 1 cut(s) 2286
BstAFI CTTAAG 1 cut(s) 1286
BstAPI GCANNNNNTGC 1 cut(s) 2603
BstAUI TGTACA 2 cut(s) 663, 1820
BstBI TTCGAA 1 cut(s) 1112
BstDEI CTNAG 4 cut(s) 1126, 1297, 1517, 3063
BstDSI CCRYGG 2 cut(s) 2640, 2937
BstENI CCTNNNNNAGG 2 cut(s) 543, 2216
BstF5I GGATG 9 cut(s) 96, 228, 448, 460, 496, 884, 930, 2739, 3044
BstFNI CGCG 1 cut(s) 126
BstH2I RGCGCY 1 cut(s) 163
BstHHI GCGC 3 cut(s) 162, 191, 1389
BstKTI GATC 8 cut(s) 36, 144, 184, 364, 919, 1780, 2404, 2483
BstMAI GTCTC 9 cut(s) 70, 217, 262, 942, 1099, 1178, 1254, 1710, 2451
BstMBI GATC 8 cut(s) 33, 141, 181, 361, 916, 1777, 2401, 2480
BstMCI CGRYCG 1 cut(s) 124
BstNI CCWGG 6 cut(s) 70, 103, 149, 224, 1003, 1761
BstNSI RCATGY 3 cut(s) 1835, 2006, 2159
BstPAI GACNNNNGTC 1 cut(s) 1493
BstSCI CCNGG 7 cut(s) 68, 101, 147, 222, 1001, 1318, 1759
BstSFI CTRYAG 5 cut(s) 80, 1218, 1628, 1646, 2979
BstUI CGCG 1 cut(s) 126
BstV2I GAAGAC 2 cut(s) 1147, 2420
BstX2I RGATCY 3 cut(s) 141, 916, 1777
BstXI CCANNNNNNTGG 1 cut(s) 1844
BstYI RGATCY 3 cut(s) 141, 916, 1777
BstZI CGGCCG 1 cut(s) 121
BsuI GTATCC 2 cut(s) 496, 639
BsuRI GGCC 4 cut(s) 100, 123, 203, 2782
BtgI CCRYGG 2 cut(s) 2640, 2937
BtrI CACGTC 1 cut(s) 2945
BtsCI GGATG 9 cut(s) 96, 228, 448, 460, 496, 884, 930, 2739, 3044
BtsI GCAGTG 2 cut(s) 89, 1658
BtsIMutI CAGTG 6 cut(s) 89, 984, 1458, 1658, 1797, 2131
BveI ACCTGC 3 cut(s) 280, 616, 658
CaiI CAGNNNCTG 6 cut(s) 983, 1205, 1304, 1679, 2282, 2350
CciI TCATGA 2 cut(s) 484, 2068
CciNI GCGGCCGC 1 cut(s) 121
CfoI GCGC 3 cut(s) 162, 191, 1389
Cfr10I RCCGGY 1 cut(s) 3005
Cfr13I GGNCC 6 cut(s) 74, 99, 1082, 2060, 2545, 2637
CseI GACGC 2 cut(s) 2075, 2294
CsiI ACCWGGT 1 cut(s) 1759
Csp6I GTAC 8 cut(s) 154, 436, 664, 1415, 1477, 1821, 2121, 2852
CviQI GTAC 8 cut(s) 154, 436, 664, 1415, 1477, 1821, 2121, 2852
DdeI CTNAG 4 cut(s) 1126, 1297, 1517, 3063
DpnI GATC 8 cut(s) 35, 143, 183, 363, 918, 1779, 2403, 2482
DpnII GATC 8 cut(s) 33, 141, 181, 361, 916, 1777, 2401, 2480
DraIII CACNNNGTG 3 cut(s) 719, 1322, 1334
DriI GACNNNNNGTC 1 cut(s) 1447
EaeI YGGCCR 1 cut(s) 121
EagI CGGCCG 1 cut(s) 121
Eam1104I CTCTTC 1 cut(s) 2835
Eam1105I GACNNNNNGTC 1 cut(s) 1447
EarI CTCTTC 1 cut(s) 2835
EciI GGCGGA 3 cut(s) 404, 467, 3037
EclXI CGGCCG 1 cut(s) 121
Eco130I CCWWGG 4 cut(s) 2233, 2540, 2633, 2937
Eco24I GRGCYC 1 cut(s) 1954
Eco31I GGTCTC 2 cut(s) 70, 262
Eco32I GATATC 2 cut(s) 1008, 1795
Eco47I GGWCC 5 cut(s) 74, 1082, 2060, 2545, 2637
Eco52I CGGCCG 1 cut(s) 121
Eco57I CTGAAG 5 cut(s) 933, 960, 1713, 2040, 2112
Eco88I CYCGRG 1 cut(s) 338
EcoNI CCTNNNNNAGG 2 cut(s) 543, 2216
EcoO109I RGGNCCY 3 cut(s) 74, 1082, 2545
EcoRI GAATTC 2 cut(s) 2394, 2535
EcoRII CCWGG 6 cut(s) 68, 101, 147, 222, 1001, 1759
EcoRV GATATC 2 cut(s) 1008, 1795
EcoT14I CCWWGG 4 cut(s) 2233, 2540, 2633, 2937
EcoT22I ATGCAT 2 cut(s) 818, 2004
EcoT38I GRGCYC 1 cut(s) 1954
ErhI CCWWGG 4 cut(s) 2233, 2540, 2633, 2937
Esp3I CGTCTC 1 cut(s) 217
FalI AAGNNNNNCTT 4 cut(s) 299, 331, 2405, 2437
FaqI GGGAC 4 cut(s) 526, 1095, 1379, 2955
FauNDI CATATG 2 cut(s) 1411, 2665
FokI GGATG 9 cut(s) 103, 215, 455, 467, 503, 891, 917, 2746, 3031
FriOI GRGCYC 1 cut(s) 1954
GlaI GCGC 3 cut(s) 161, 190, 1388
GsaI CCCAGC 2 cut(s) 832, 1892
GsuI CTGGAG 2 cut(s) 206, 1692
HaeII RGCGCY 1 cut(s) 163
HaeIII GGCC 4 cut(s) 100, 123, 203, 2782
HapII CCGG 6 cut(s) 396, 993, 1319, 2199, 2715, 3006
HgaI GACGC 2 cut(s) 2075, 2294
HhaI GCGC 3 cut(s) 162, 191, 1389
Hin1I GRCGYC 1 cut(s) 2286
Hin6I GCGC 3 cut(s) 160, 189, 1387
HinP1I GCGC 3 cut(s) 160, 189, 1387
HincII GTYRAC 2 cut(s) 1488, 2824
HindII GTYRAC 2 cut(s) 1488, 2824
HindIII AAGCTT 2 cut(s) 1115, 2746
HpaII CCGG 6 cut(s) 396, 993, 1319, 2199, 2715, 3006
HphI GGTGA 4 cut(s) 410, 1596, 1775, 2125
Hpy166II GTNNAC 4 cut(s) 262, 1488, 1823, 2824
Hpy8I GTNNAC 4 cut(s) 262, 1488, 1823, 2824
HpyCH4III ACNGT 6 cut(s) 377, 1219, 1248, 1681, 1867, 2140
HpyCH4IV ACGT 3 cut(s) 366, 1601, 2944
HpyF3I CTNAG 4 cut(s) 1126, 1297, 1517, 3063
HpySE526I ACGT 3 cut(s) 366, 1601, 2944
Hsp92I GRCGYC 1 cut(s) 2286
HspAI GCGC 3 cut(s) 160, 189, 1387
Kzo9I GATC 8 cut(s) 33, 141, 181, 361, 916, 1777, 2401, 2480
MabI ACCWGGT 1 cut(s) 1759
MaeII ACGT 3 cut(s) 366, 1601, 2944
MaeIII GTNAC 9 cut(s) 707, 899, 1458, 1602, 1847, 2126, 2178, 2554, 2668
MalI GATC 8 cut(s) 35, 143, 183, 363, 918, 1779, 2403, 2482
MboI GATC 8 cut(s) 33, 141, 181, 361, 916, 1777, 2401, 2480
MfeI CAATTG 1 cut(s) 2598
MflI RGATCY 3 cut(s) 141, 916, 1777
MhlI GDGCHC 5 cut(s) 132, 1954, 2052, 2581, 2743
MlyI GAGTC 5 cut(s) 14, 200, 1401, 1712, 1947
MmeI TCCRAC 3 cut(s) 240, 336, 636
Mph1103I ATGCAT 2 cut(s) 818, 2004
MroXI GAANNNNTTC 4 cut(s) 1527, 1875, 2206, 2802
MslI CAYNNNNRTG 2 cut(s) 489, 2475
MspA1I CMGCKG 1 cut(s) 2984
MspCI CTTAAG 1 cut(s) 1286
MspI CCGG 6 cut(s) 396, 993, 1319, 2199, 2715, 3006
MspR9I CCNGG 7 cut(s) 70, 103, 149, 224, 1003, 1320, 1761
MunI CAATTG 1 cut(s) 2598
Mva1269I GAATGC 2 cut(s) 850, 906
MvaI CCWGG 6 cut(s) 70, 103, 149, 224, 1003, 1761
MvnI CGCG 1 cut(s) 126
NciI CCSGG 1 cut(s) 1320
NcoI CCATGG 1 cut(s) 2937
NdeI CATATG 2 cut(s) 1411, 2665
NdeII GATC 8 cut(s) 33, 141, 181, 361, 916, 1777, 2401, 2480
NmeAIII GCCGAG 1 cut(s) 87
NmuCI GTSAC 5 cut(s) 707, 1458, 1602, 2126, 2668
NotI GCGGCCGC 1 cut(s) 121
NsiI ATGCAT 2 cut(s) 818, 2004
NspI RCATGY 3 cut(s) 1835, 2006, 2159
NspV TTCGAA 1 cut(s) 1112
PaeI GCATGC 1 cut(s) 1835
PaeR7I CTCGAG 1 cut(s) 338
PagI TCATGA 2 cut(s) 484, 2068
PcsI WCGNNNNNNNCGW 1 cut(s) 1779
PctI GAATGC 2 cut(s) 850, 906
PdmI GAANNNNTTC 4 cut(s) 1527, 1875, 2206, 2802
PflFI GACNNNGTC 1 cut(s) 209
PfoI TCCNGGA 1 cut(s) 222
PleI GAGTC 5 cut(s) 13, 200, 1400, 1711, 1946
PpsI GAGTC 5 cut(s) 13, 200, 1400, 1711, 1946
PpuMI RGGWCCY 3 cut(s) 74, 1082, 2545
PshAI GACNNNNGTC 1 cut(s) 1493
PsiI TTATAA 1 cut(s) 960
Psp5II RGGWCCY 3 cut(s) 74, 1082, 2545
Psp6I CCWGG 6 cut(s) 68, 101, 147, 222, 1001, 1759
PspFI CCCAGC 2 cut(s) 828, 1888
PspGI CCWGG 6 cut(s) 68, 101, 147, 222, 1001, 1759
PspPI GGNCC 6 cut(s) 74, 99, 1082, 2060, 2545, 2637
PspPPI RGGWCCY 3 cut(s) 74, 1082, 2545
PsrI GAACNNNNNNTAC 2 cut(s) 1840, 1872
PstI CTGCAG 2 cut(s) 84, 2983
PstNI CAGNNNCTG 6 cut(s) 983, 1205, 1304, 1679, 2282, 2350
PsuI RGATCY 3 cut(s) 141, 916, 1777
PsyI GACNNNGTC 1 cut(s) 209
PvuII CAGCTG 1 cut(s) 2984
RsaI GTAC 8 cut(s) 155, 437, 665, 1416, 1478, 1822, 2122, 2853
RsaNI GTAC 8 cut(s) 154, 436, 664, 1415, 1477, 1821, 2121, 2852
RseI CAYNNNNRTG 2 cut(s) 489, 2475
Sau3AI GATC 8 cut(s) 33, 141, 181, 361, 916, 1777, 2401, 2480
Sau96I GGNCC 6 cut(s) 74, 99, 1082, 2060, 2545, 2637
ScaI AGTACT 1 cut(s) 1478
SchI GAGTC 5 cut(s) 14, 200, 1401, 1712, 1947
ScrFI CCNGG 7 cut(s) 70, 103, 149, 224, 1003, 1320, 1761
SduI GDGCHC 5 cut(s) 132, 1954, 2052, 2581, 2743
SexAI ACCWGGT 1 cut(s) 1759
SfcI CTRYAG 5 cut(s) 80, 1218, 1628, 1646, 2979
Sfr274I CTCGAG 1 cut(s) 338
SfuI TTCGAA 1 cut(s) 1112
SinI GGWCC 5 cut(s) 74, 1082, 2060, 2545, 2637
SlaI CTCGAG 1 cut(s) 338
SmiMI CAYNNNNRTG 2 cut(s) 489, 2475
SmlI CTYRAG 6 cut(s) 59, 338, 1286, 2189, 2952, 3052
SmoI CTYRAG 6 cut(s) 59, 338, 1286, 2189, 2952, 3052
SphI GCATGC 1 cut(s) 1835
SspI AATATT 1 cut(s) 2260
StyD4I CCNGG 7 cut(s) 68, 101, 147, 222, 1001, 1318, 1759
StyI CCWWGG 4 cut(s) 2233, 2540, 2633, 2937
TaaI ACNGT 6 cut(s) 377, 1219, 1248, 1681, 1867, 2140
TaiI ACGT 3 cut(s) 369, 1604, 2947
TaqI TCGA 3 cut(s) 8, 339, 1112
TaqII GACCGA 3 cut(s) 1483, 2174, 2432
TatI WGTACW 5 cut(s) 435, 663, 1476, 1820, 2851
TauI GCSGC 9 cut(s) 123, 126, 138, 165, 168, 194, 614, 1380, 2782
TscAI CASTG 6 cut(s) 89, 984, 1458, 1658, 1804, 2131
TseFI GTSAC 5 cut(s) 707, 1458, 1602, 2126, 2668
Tsp45I GTSAC 5 cut(s) 707, 1458, 1602, 2126, 2668
TspGWI ACGGA 3 cut(s) 199, 703, 1788
TspRI CASTG 6 cut(s) 89, 984, 1458, 1658, 1804, 2131
Tth111I GACNNNGTC 1 cut(s) 209
Vha464I CTTAAG 1 cut(s) 1286
VpaK11BI GGWCC 5 cut(s) 74, 1082, 2060, 2545, 2637
XagI CCTNNNNNAGG 2 cut(s) 543, 2216
XapI RAATTY 8 cut(s) 565, 2105, 2338, 2353, 2394, 2535, 2564, 2965
XceI RCATGY 3 cut(s) 1835, 2006, 2159
XcmI CCANNNNNNNNNTGG 1 cut(s) 836
XhoI CTCGAG 1 cut(s) 338
XmnI GAANNNNTTC 4 cut(s) 1527, 1875, 2206, 2802
ZrmI AGTACT 1 cut(s) 1478
Zsp2I ATGCAT 2 cut(s) 818, 2004
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.