AT3G07450

Protease inhibitor seed storage lipid transfer protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
3
OG0016698
Physical Location & Seq
Reverse (-)
2383442 .. 2383937
496 bp
Loading structure...
UTR
Exon/CDS
Intron
AT3G07450.1

Sequence Viewer

Length: 321 bp
ATGATTGTAATGAAAAAGAGTCAGGTTTTTACAGTGATGTTACTTATGGTTATGGTCCAGTCAGGTCTGGTGAGAGAGACGCTGGGACACCCATGTGGTCGTACATTTTTGTCGGCTTTGATTCAGCTAGTGCCGTGTAGACCATCAGTGGCTCCGTTTAGCACATTGCCTCCGAACGGGCTATGTTGCGCTGCCATCAAGACACTTGGTCAACCTTGTTTATGCGTTCTTGCCAAGGGACCACCCATCGTTGGCGTTGACCGGACCCTCGCTCTCCATTTACCTGGAAAATGTTCTGCCAATTTTCTTCCATGTAATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LTP_2

Protein Analysis

106

Amino Acids

11.23

Weight (kDa)

9.27

Isoelectric Point (pI)

24.53

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LTP_2 PF14368 18 - 100 1.1e-06 Probable lipid transfer
Tryp_alpha_amyl PF00234 37 - 104 6.7e-08 Protease inhibitor/seed storage/LTP family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016698)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G07450
fragaria_vesca FvH4_7g00620
malus_domestica MD02G1316200.v1.1 MD07G1005700.v1.1
prunus_persica Prupe.2G008100_v2.0.a1 Prupe.2G008300_v2.0.a1
rosa_chinensis RchiOBHm_Chr1g0314811
rosa_multiflora Rmu_sc0025729.1_g000001
rosa_rugosa Rorug01G0002000
rosa_samantha Rh1AG012900 Rh1BG004500 Rh1CG011400 Rh1DG006200
rosa_wichuraiana Rw1G000550

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 139
AfaI GTAC 1 cut(s) 103
AfiI CCNNNNNNNGG 2 cut(s) 176, 251
AhdI GACNNNNNGTC 1 cut(s) 207
AjnI CCWGG 1 cut(s) 283
AleI CACNNNNGTG 1 cut(s) 93
AluBI AGCT 1 cut(s) 127
AluI AGCT 1 cut(s) 127
Alw26I GTCTC 1 cut(s) 71
ApeKI GCWGC 1 cut(s) 191
Asp700I GAANNNNTTC 1 cut(s) 292
AspLEI GCGC 1 cut(s) 191
AspS9I GGNCC 3 cut(s) 55, 239, 264
AsuHPI GGTGA 1 cut(s) 82
AvaII GGWCC 3 cut(s) 55, 239, 264
BbvI GCAGC 1 cut(s) 178
BccI CCATC 3 cut(s) 151, 203, 254
BceAI ACGGC 1 cut(s) 118
BciT130I CCWGG 1 cut(s) 285
BcoDI GTCTC 1 cut(s) 71
BfaI CTAG 1 cut(s) 128
BisI GCNGC 1 cut(s) 192
BlsI GCNGC 1 cut(s) 193
Bme1390I CCNGG 1 cut(s) 285
Bme18I GGWCC 3 cut(s) 55, 239, 264
BmeRI GACNNNNNGTC 1 cut(s) 207
BmgT120I GGNCC 3 cut(s) 55, 239, 264
BmiI GGNNCC 3 cut(s) 153, 240, 266
BmrFI CCNGG 1 cut(s) 285
BsaJI CCNNGG 1 cut(s) 234
BsaWI WCCGGW 1 cut(s) 261
BsaXI ACNNNNNCTCC 2 cut(s) 154, 184
Bsc4I CCNNNNNNNGG 2 cut(s) 176, 251
Bse1I ACTGG 1 cut(s) 58
Bse3DI GCAATG 1 cut(s) 164
BseBI CCWGG 1 cut(s) 285
BseDI CCNNGG 1 cut(s) 234
BseLI CCNNNNNNNGG 2 cut(s) 176, 251
BseMI GCAATG 1 cut(s) 164
BseNI ACTGG 1 cut(s) 58
BseXI GCAGC 1 cut(s) 178
BseYI CCCAGC 1 cut(s) 82
BsiSI CCGG 1 cut(s) 262
BslFI GGGAC 2 cut(s) 99, 252
BslI CCNNNNNNNGG 2 cut(s) 176, 251
BsmAI GTCTC 1 cut(s) 71
BsmBI CGTCTC 1 cut(s) 71
BsmFI GGGAC 2 cut(s) 99, 252
BspLI GGNNCC 3 cut(s) 153, 240, 266
BsrDI GCAATG 1 cut(s) 164
BsrI ACTGG 1 cut(s) 58
BssECI CCNNGG 1 cut(s) 234
BssT1I CCWWGG 1 cut(s) 234
Bst2UI CCWGG 1 cut(s) 285
Bst4CI ACNGT 1 cut(s) 34
BstHHI GCGC 1 cut(s) 191
BstMAI GTCTC 1 cut(s) 71
BstNI CCWGG 1 cut(s) 285
BstSCI CCNGG 1 cut(s) 283
BstV1I GCAGC 1 cut(s) 178
BstXI CCANNNNNNTGG 1 cut(s) 284
BtsIMutI CAGTG 2 cut(s) 39, 153
CfoI GCGC 1 cut(s) 191
Cfr13I GGNCC 3 cut(s) 55, 239, 264
CseI GACGC 1 cut(s) 88
Csp6I GTAC 1 cut(s) 102
CviAII CATG 2 cut(s) 93, 312
CviJI RGCY 4 cut(s) 116, 127, 152, 181
CviKI_1 RGCY 4 cut(s) 116, 127, 152, 181
CviQI GTAC 1 cut(s) 102
DriI GACNNNNNGTC 1 cut(s) 207
Eam1105I GACNNNNNGTC 1 cut(s) 207
Eco130I CCWWGG 1 cut(s) 234
Eco47I GGWCC 3 cut(s) 55, 239, 264
EcoRII CCWGG 1 cut(s) 283
EcoT14I CCWWGG 1 cut(s) 234
ErhI CCWWGG 1 cut(s) 234
Esp3I CGTCTC 1 cut(s) 71
FaeI CATG 2 cut(s) 96, 315
FaiI YATR 6 cut(s) 47, 53, 94, 184, 223, 313
FaqI GGGAC 2 cut(s) 99, 252
FatI CATG 2 cut(s) 92, 311
FblI GTMKAC 1 cut(s) 139
Fnu4HI GCNGC 1 cut(s) 192
Fsp4HI GCNGC 1 cut(s) 192
FspBI CTAG 1 cut(s) 128
GlaI GCGC 1 cut(s) 190
GluI GCNGC 1 cut(s) 192
GsaI CCCAGC 1 cut(s) 86
HapII CCGG 1 cut(s) 262
HgaI GACGC 1 cut(s) 88
HhaI GCGC 1 cut(s) 191
Hin1II CATG 2 cut(s) 96, 315
Hin6I GCGC 1 cut(s) 189
HinP1I GCGC 1 cut(s) 189
HincII GTYRAC 2 cut(s) 212, 259
HindII GTYRAC 2 cut(s) 212, 259
HinfI GANTC 2 cut(s) 19, 121
HpaII CCGG 1 cut(s) 262
HphI GGTGA 1 cut(s) 82
Hpy166II GTNNAC 3 cut(s) 140, 212, 259
Hpy188I TCNGA 1 cut(s) 174
Hpy188III TCNNGA 1 cut(s) 199
Hpy8I GTNNAC 3 cut(s) 140, 212, 259
HpyCH4III ACNGT 1 cut(s) 34
Hsp92II CATG 2 cut(s) 96, 315
HspAI GCGC 1 cut(s) 189
LmnI GCTCC 1 cut(s) 157
LpnPI CCDG 8 cut(s) 8, 48, 53, 68, 71, 270, 275, 297
Lsp1109I GCAGC 1 cut(s) 178
MaeI CTAG 1 cut(s) 128
MaeIII GTNAC 1 cut(s) 39
MboII GAAGA 1 cut(s) 299
MluCI AATT 2 cut(s) 301, 316
MlyI GAGTC 1 cut(s) 28
MnlI CCTC 2 cut(s) 180, 278
MroXI GAANNNNTTC 1 cut(s) 292
MseI TTAA 1 cut(s) 319
MslI CAYNNNNRTG 1 cut(s) 93
MspI CCGG 1 cut(s) 262
MspR9I CCNGG 1 cut(s) 285
MvaI CCWGG 1 cut(s) 285
NlaIII CATG 2 cut(s) 96, 315
NlaIV GGNNCC 3 cut(s) 153, 240, 266
OliI CACNNNNGTG 1 cut(s) 93
PdmI GAANNNNTTC 1 cut(s) 292
PfeI GAWTC 1 cut(s) 121
PkrI GCNGC 1 cut(s) 193
PleI GAGTC 1 cut(s) 27
PpsI GAGTC 1 cut(s) 27
Psp6I CCWGG 1 cut(s) 283
PspFI CCCAGC 1 cut(s) 82
PspGI CCWGG 1 cut(s) 283
PspN4I GGNNCC 3 cut(s) 153, 240, 266
PspPI GGNCC 3 cut(s) 55, 239, 264
RsaI GTAC 1 cut(s) 103
RsaNI GTAC 1 cut(s) 102
RseI CAYNNNNRTG 1 cut(s) 93
SaqAI TTAA 1 cut(s) 319
SatI GCNGC 1 cut(s) 192
Sau96I GGNCC 3 cut(s) 55, 239, 264
SchI GAGTC 1 cut(s) 28
ScrFI CCNGG 1 cut(s) 285
SetI ASST 5 cut(s) 27, 67, 129, 217, 286
SinI GGWCC 3 cut(s) 55, 239, 264
SmiMI CAYNNNNRTG 1 cut(s) 93
Sse9I AATT 2 cut(s) 301, 316
SspMI CTAG 1 cut(s) 128
StyD4I CCNGG 1 cut(s) 283
StyI CCWWGG 1 cut(s) 234
TaaI ACNGT 1 cut(s) 34
TasI AATT 2 cut(s) 301, 316
TfiI GAWTC 1 cut(s) 121
Tru1I TTAA 1 cut(s) 319
Tru9I TTAA 1 cut(s) 319
TscAI CASTG 2 cut(s) 39, 153
TseI GCWGC 1 cut(s) 191
TspDTI ATGAA 1 cut(s) 26
TspGWI ACGGA 1 cut(s) 144
TspRI CASTG 2 cut(s) 39, 153
VpaK11BI GGWCC 3 cut(s) 55, 239, 264
XmiI GTMKAC 1 cut(s) 139
XmnI GAANNNNTTC 1 cut(s) 292
XspI CTAG 1 cut(s) 128
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.