Basic Information

Type: gene

Biological Identity

Species

arabidopsis_thaliana

Chromosome

Orthogroup ID

OG0014000

Physical Location & Seq

Genomic Coordinates Forward (+)

3199712 .. 3201966

Gene Structure 2255 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

AT3G10330.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 939 bp

ATGAGTGACGCGTTTTGTTCGGACTGTAAGAGGCACACGGAGGTTGTGTTTGACCATTCCGCCGGAGATACAGTTTGTTCAGAGTGTGGTCTCGTGCTTGAATCTCACTCAATCGATGAAACTTCTGAGTGGCGTACTTTCGCTAATGAGTCCGGTGATAATGATCCTGTTCGTGTCGGTGGTCCGACGAATCCTCTTTTAGCAGATGGTGGTCTCACTACTGTCATTTCTAAACCTAATGGATCCTCTGGCGATTTCCTCTCTTCTTCTCTTGGTCGATGGCAGAATCGTGGATCTAATCCTGATCGTGGCCTTATTGTTGCTTTCAAGACTATCGCTACCATGGCTGATAGGTTGGGACTTGTTGCGACCATCAAGGACCGAGCGAATGAGATTTATAAAAGGGTAGAGGATCAGAAGTCAAGTAGAGGAAGAAATCAGGATGCTCTTTTGGCTGCTTGTTTATACATTGCTTGCCGGCAAGAAGACAAACCACGGACGGTCAAGGAAATATGCTCTGTTGCCAATGGAGCTACTAAGAAGGAGATTGGTCGTGCAAAAGAGTACATAGTCAAACAGTTGGGGCTGGAAACCGGACAATTGGTTGAAATGGGTACTATACATGCTGGAGATTTTATGAGACGTTTTTGCTCCAATCTTGGTATGACCAACCAAACCGTGAAAGCGGCTCAAGAGTCTGTGCAAAAGTCAGAAGAATTTGATATAAGGAGGAGTCCCATATCGATTGCAGCAGCGGTTATATACATCATAACACAGCTCTCTGATGAGAAGAAGCCTCTTCGAGATATATCAGTGGCAACTGGAGTCGCGGAAGGAACTATAAGAAACTCTTACAAAGATCTTTACCCTCACCTTTCCAAGATCATACCAGCTTGGTACGCTAAAGAAGAAGATCTCAAGAACCTTCAAAGCCCTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0001558 GO:0003006 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0006935 GO:0007275 GO:0008150 GO:0009605 GO:0009791 GO:0009856 GO:0009888 GO:0009960 GO:0010154 GO:0010183 GO:0010769 GO:0022414 GO:0022603 GO:0022604 GO:0032501 GO:0032502 GO:0040008 GO:0040011 GO:0042221 GO:0042330 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043900 GO:0044424 GO:0044464 GO:0044706 GO:0045595 GO:0048316 GO:0048608 GO:0048638 GO:0048731 GO:0048856 GO:0048868 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0050918 GO:0051128 GO:0051239 GO:0051510 GO:0051704 GO:0060284 GO:0061458 GO:0065007 GO:0080092 GO:2000241

KEGG Pathways

Metabolic & Signaling

ko03022 ko05169 ko05203 map03022 map05169 map05203

Pfam Domains

Protein Families

TFIIB TF_Zn_Ribbon

Protein Analysis

312

Amino Acids

34.22

Weight (kDa)

6.26

Isoelectric Point (pI)

41.84

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Zn_Ribbon_TF	PF08271	5 - 47	1.4e-15	TFIIB zinc-binding
TFIIB	PF00382	103 - 185	2.8e-21	Transcription factor TFIIB repeat
TFIIB	PF00382	204 - 290	1.7e-13	Transcription factor TFIIB repeat

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0014000)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G10330
fragaria_vesca	FvH4_6g24330
malus_domestica	MD12G1075300.v1.1 MD14G1073800.v1.1
prunus_persica	Prupe.7G055400_v2.0.a1
pyrus_communis	pycom12g06480 pycom14g06300
rosa_chinensis	RchiOBHm_Chr3g0483261
rosa_laevigata	RLG00000023294
rosa_multiflora	Rmu_sc0007550.1_g000009
rosa_roxburghii	Rroxscaffold_6G00398520
rosa_rugosa	Rorug03G0205000
rosa_samantha	Rh3AG253400 Rh3BG290500 Rh3CG287300 Rh3DG283100
rosa_wichuraiana	Rw3G022920

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	399
AccII	CGCG	2 cut(s)	11, 830
AciI	CCGC	4 cut(s)	60, 686, 755, 830
AclWI	GGATC	5 cut(s)	158, 237, 250, 301, 420
AcsI	RAATTY	1 cut(s)	716
AfaI	GTAC	4 cut(s)	136, 566, 616, 899
AfiI	CCNNNNNNNGG	1 cut(s)	308
AflIII	ACRYGT	1 cut(s)	9
AgsI	TTSAA	4 cut(s)	101, 328, 608, 929
AluBI	AGCT	3 cut(s)	533, 778, 893
AluI	AGCT	3 cut(s)	533, 778, 893
Alw26I	GTCTC	3 cut(s)	95, 218, 634
AlwI	GGATC	5 cut(s)	158, 237, 250, 301, 420
AoxI	GGCC	1 cut(s)	310
ApeKI	GCWGC	3 cut(s)	455, 749, 752
ApoI	RAATTY	1 cut(s)	716
AspS9I	GGNCC	2 cut(s)	182, 379
AsuHPI	GGTGA	2 cut(s)	167, 863
AvaII	GGWCC	2 cut(s)	182, 379
BamHI	GGATCC	1 cut(s)	242
BauI	CACGAG	1 cut(s)	92
BbsI	GAAGAC	1 cut(s)	492
BbvI	GCAGC	3 cut(s)	442, 761, 764
BccI	CCATC	3 cut(s)	200, 273, 380
BcoDI	GTCTC	3 cut(s)	95, 218, 634
BglII	AGATCT	2 cut(s)	859, 913
BisI	GCNGC	4 cut(s)	456, 687, 750, 753
BlsI	GCNGC	4 cut(s)	457, 688, 751, 754
Bme18I	GGWCC	2 cut(s)	182, 379
BmgT120I	GGNCC	2 cut(s)	182, 379
BmiI	GGNNCC	1 cut(s)	244
BmsI	GCATC	1 cut(s)	433
BpiI	GAAGAC	1 cut(s)	492
BpmI	CTGGAG	2 cut(s)	648, 843
BpuEI	CTTGAG	2 cut(s)	675, 902
Bsa29I	ATCGAT	2 cut(s)	114, 743
BsaBI	GATNNNNATC	1 cut(s)	162
BsaI	GGTCTC	2 cut(s)	95, 218
BsaJI	CCNNGG	2 cut(s)	342, 494
BsaWI	WCCGGW	2 cut(s)	152, 593
BsaXI	ACNNNNNCTCC	4 cut(s)	32, 57, 62, 87
Bsc4I	CCNNNNNNNGG	1 cut(s)	308
Bse118I	RCCGGY	1 cut(s)	477
Bse1I	ACTGG	1 cut(s)	826
Bse3DI	GCAATG	1 cut(s)	468
Bse8I	GATNNNNATC	1 cut(s)	162
BseCI	ATCGAT	2 cut(s)	114, 743
BseDI	CCNNGG	2 cut(s)	342, 494
BseGI	GGATG	1 cut(s)	448
BseJI	GATNNNNATC	1 cut(s)	162
BseLI	CCNNNNNNNGG	1 cut(s)	308
BseMI	GCAATG	1 cut(s)	468
BseMII	CTCAG	1 cut(s)	117
BseNI	ACTGG	1 cut(s)	826
BseRI	GAGGAG	1 cut(s)	745
BseXI	GCAGC	3 cut(s)	442, 761, 764
Bsh1236I	CGCG	2 cut(s)	11, 830
BshFI	GGCC	1 cut(s)	312
BshVI	ATCGAT	2 cut(s)	114, 743
BsiSI	CCGG	4 cut(s)	63, 153, 478, 594
BslFI	GGGAC	2 cut(s)	372, 720
BslI	CCNNNNNNNGG	1 cut(s)	308
BsmAI	GTCTC	3 cut(s)	95, 218, 634
BsmBI	CGTCTC	1 cut(s)	634
BsmFI	GGGAC	2 cut(s)	372, 720
BsnI	GGCC	1 cut(s)	312
Bso31I	GGTCTC	2 cut(s)	95, 218
Bsp143I	GATC	8 cut(s)	163, 242, 293, 304, 412, 859, 882, 913
Bsp19I	CCATGG	1 cut(s)	342
BspACI	CCGC	4 cut(s)	60, 686, 755, 830
BspANI	GGCC	1 cut(s)	312
BspCNI	CTCAG	1 cut(s)	118
BspDI	ATCGAT	2 cut(s)	114, 743
BspFNI	CGCG	2 cut(s)	11, 830
BspLI	GGNNCC	1 cut(s)	244
BspPI	GGATC	5 cut(s)	158, 237, 250, 301, 420
BspTNI	GGTCTC	2 cut(s)	95, 218
BsrDI	GCAATG	1 cut(s)	468
BsrFI	RCCGGY	1 cut(s)	477
BsrI	ACTGG	1 cut(s)	826
BssAI	RCCGGY	1 cut(s)	477
BssECI	CCNNGG	2 cut(s)	342, 494
BssMI	GATC	8 cut(s)	163, 242, 293, 304, 412, 859, 882, 913
BssSI	CACGAG	1 cut(s)	92
BssT1I	CCWWGG	1 cut(s)	342
Bst2BI	CACGAG	1 cut(s)	92
Bst4CI	ACNGT	6 cut(s)	26, 73, 223, 502, 579, 679
Bst6I	CTCTTC	2 cut(s)	268, 804
BstC8I	GCNNGC	2 cut(s)	475, 479
BstDEI	CTNAG	2 cut(s)	126, 537
BstDSI	CCRYGG	2 cut(s)	342, 494
BstF5I	GGATG	1 cut(s)	448
BstFNI	CGCG	2 cut(s)	11, 830
BstKTI	GATC	8 cut(s)	166, 245, 296, 307, 415, 862, 885, 916
BstMAI	GTCTC	3 cut(s)	95, 218, 634
BstMBI	GATC	8 cut(s)	163, 242, 293, 304, 412, 859, 882, 913
BstMWI	GCNNNNNNNGC	4 cut(s)	344, 452, 530, 899
BstNSI	RCATGY	1 cut(s)	626
BstUI	CGCG	2 cut(s)	11, 830
BstV1I	GCAGC	3 cut(s)	442, 761, 764
BstV2I	GAAGAC	1 cut(s)	492
BstX2I	RGATCY	4 cut(s)	242, 293, 859, 913
BstYI	RGATCY	4 cut(s)	242, 293, 859, 913
Bsu15I	ATCGAT	2 cut(s)	114, 743
BsuRI	GGCC	1 cut(s)	312
BsuTUI	ATCGAT	2 cut(s)	114, 743
BtgI	CCRYGG	2 cut(s)	342, 494
BtsCI	GGATG	1 cut(s)	448
BtsIMutI	CAGTG	1 cut(s)	819
Cac8I	GCNNGC	2 cut(s)	475, 479
Cfr10I	RCCGGY	1 cut(s)	477
Cfr13I	GGNCC	2 cut(s)	182, 379
ClaI	ATCGAT	2 cut(s)	114, 743
CseI	GACGC	1 cut(s)	17
Csp6I	GTAC	4 cut(s)	135, 565, 615, 898
CviAII	CATG	2 cut(s)	343, 623
CviQI	GTAC	4 cut(s)	135, 565, 615, 898
DdeI	CTNAG	2 cut(s)	126, 537
DpnI	GATC	8 cut(s)	165, 244, 295, 306, 414, 861, 884, 915
DpnII	GATC	8 cut(s)	163, 242, 293, 304, 412, 859, 882, 913
Eam1104I	CTCTTC	2 cut(s)	268, 804
EarI	CTCTTC	2 cut(s)	268, 804
EciI	GGCGGA	1 cut(s)	49
Eco130I	CCWWGG	1 cut(s)	342
Eco31I	GGTCTC	2 cut(s)	95, 218
Eco47I	GGWCC	2 cut(s)	182, 379
EcoT14I	CCWWGG	1 cut(s)	342
ErhI	CCWWGG	1 cut(s)	342
Esp3I	CGTCTC	1 cut(s)	634
FaeI	CATG	2 cut(s)	346, 626
FalI	AAGNNNNNCTT	2 cut(s)	835, 867
FaqI	GGGAC	2 cut(s)	372, 720
FatI	CATG	2 cut(s)	342, 622
Fnu4HI	GCNGC	4 cut(s)	456, 687, 750, 753
FokI	GGATG	1 cut(s)	455
Fsp4HI	GCNGC	4 cut(s)	456, 687, 750, 753
GluI	GCNGC	4 cut(s)	456, 687, 750, 753
GsuI	CTGGAG	2 cut(s)	648, 843
HaeIII	GGCC	1 cut(s)	312
HapII	CCGG	4 cut(s)	63, 153, 478, 594
HgaI	GACGC	1 cut(s)	17
Hin1II	CATG	2 cut(s)	346, 626
HinfI	GANTC	7 cut(s)	101, 149, 190, 286, 695, 733, 825
HpaII	CCGG	4 cut(s)	63, 153, 478, 594
HphI	GGTGA	2 cut(s)	167, 863
Hpy188I	TCNGA	7 cut(s)	22, 82, 127, 186, 417, 712, 784
Hpy188III	TCNNGA	6 cut(s)	302, 328, 440, 692, 803, 919
Hpy99I	CGWCG	1 cut(s)	190
HpyAV	CCTTC	3 cut(s)	535, 827, 935
HpyCH4III	ACNGT	6 cut(s)	26, 73, 223, 502, 579, 679
HpyCH4IV	ACGT	1 cut(s)	643
HpyCH4V	TGCA	3 cut(s)	557, 703, 749
HpyF10VI	GCNNNNNNNGC	4 cut(s)	344, 452, 530, 899
HpyF3I	CTNAG	2 cut(s)	126, 537
HpySE526I	ACGT	1 cut(s)	643
Hsp92II	CATG	2 cut(s)	346, 626
KroI	GCCGGC	1 cut(s)	477
KroNI	GCCGGC	1 cut(s)	479
Kzo9I	GATC	8 cut(s)	163, 242, 293, 304, 412, 859, 882, 913
LmnI	GCTCC	2 cut(s)	530, 656
Lsp1109I	GCAGC	3 cut(s)	442, 761, 764
LweI	GCATC	1 cut(s)	433
MaeII	ACGT	1 cut(s)	643
MaeIII	GTNAC	1 cut(s)	5
MalI	GATC	8 cut(s)	165, 244, 295, 306, 414, 861, 884, 915
MboI	GATC	8 cut(s)	163, 242, 293, 304, 412, 859, 882, 913
MboII	GAAGA	9 cut(s)	255, 258, 444, 497, 725, 791, 802, 920, 923
MfeI	CAATTG	1 cut(s)	599
MflI	RGATCY	4 cut(s)	242, 293, 859, 913
MluCI	AATT	2 cut(s)	599, 716
MluI	ACGCGT	1 cut(s)	9
MlyI	GAGTC	4 cut(s)	158, 704, 742, 834
MmeI	TCCRAC	1 cut(s)	209
MroNI	GCCGGC	1 cut(s)	477
MspA1I	CMGCKG	1 cut(s)	755
MspI	CCGG	4 cut(s)	63, 153, 478, 594
MunI	CAATTG	1 cut(s)	599
MvnI	CGCG	2 cut(s)	11, 830
MwoI	GCNNNNNNNGC	4 cut(s)	344, 452, 530, 899
NaeI	GCCGGC	1 cut(s)	479
NcoI	CCATGG	1 cut(s)	342
NdeII	GATC	8 cut(s)	163, 242, 293, 304, 412, 859, 882, 913
NgoMIV	GCCGGC	1 cut(s)	477
NlaIII	CATG	2 cut(s)	346, 626
NlaIV	GGNNCC	1 cut(s)	244
NmuCI	GTSAC	1 cut(s)	5
NspI	RCATGY	1 cut(s)	626
PdiI	GCCGGC	1 cut(s)	479
PfeI	GAWTC	3 cut(s)	101, 190, 286
PkrI	GCNGC	4 cut(s)	457, 688, 751, 754
PleI	GAGTC	4 cut(s)	157, 703, 741, 833
PpsI	GAGTC	4 cut(s)	157, 703, 741, 833
PsiI	TTATAA	1 cut(s)	399
PspN4I	GGNNCC	1 cut(s)	244
PspPI	GGNCC	2 cut(s)	182, 379
PsuI	RGATCY	4 cut(s)	242, 293, 859, 913
RsaI	GTAC	4 cut(s)	136, 566, 616, 899
RsaNI	GTAC	4 cut(s)	135, 565, 615, 898
SatI	GCNGC	4 cut(s)	456, 687, 750, 753
Sau3AI	GATC	8 cut(s)	163, 242, 293, 304, 412, 859, 882, 913
Sau96I	GGNCC	2 cut(s)	182, 379
SchI	GAGTC	4 cut(s)	158, 704, 742, 834
SetI	ASST	9 cut(s)	45, 238, 356, 535, 646, 780, 876, 895, 927
SfaNI	GCATC	1 cut(s)	433
SinI	GGWCC	2 cut(s)	182, 379
SmlI	CTYRAG	2 cut(s)	690, 917
SmoI	CTYRAG	2 cut(s)	690, 917
Sse9I	AATT	2 cut(s)	599, 716
SsiI	CCGC	4 cut(s)	60, 686, 755, 830
StyI	CCWWGG	1 cut(s)	342
TaaI	ACNGT	6 cut(s)	26, 73, 223, 502, 579, 679
TaiI	ACGT	1 cut(s)	646
TaqI	TCGA	4 cut(s)	114, 277, 743, 802
TaqII	GACCGA	1 cut(s)	396
TasI	AATT	2 cut(s)	599, 716
TatI	WGTACW	1 cut(s)	564
TauI	GCSGC	1 cut(s)	689
TfiI	GAWTC	3 cut(s)	101, 190, 286
TscAI	CASTG	1 cut(s)	819
TseFI	GTSAC	1 cut(s)	5
TseI	GCWGC	3 cut(s)	455, 749, 752
Tsp45I	GTSAC	1 cut(s)	5
TspDTI	ATGAA	1 cut(s)	132
TspGWI	ACGGA	2 cut(s)	53, 511
TspRI	CASTG	1 cut(s)	819
VpaK11BI	GGWCC	2 cut(s)	182, 379
XapI	RAATTY	1 cut(s)	716
XceI	RCATGY	1 cut(s)	626

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.