Rorug03G0205000

Transcription initiation factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000003
OG0014000
Physical Location & Seq
Forward (+)
18186085 .. 18187140
1056 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug03G0205000.1

Sequence Viewer

Length: 651 bp
ATGGACTCCACCAGCTTCTGCGACCGCCGATCGCCGTCGTCGGACCGCTTCCTCGGCCTCTTCTCCTTCTCGCTGCCCTCCTCCGCCGCCGGCGAGGAGCTCAACGAGGCCGAGGTCTTCTGGACGACCACCGACGACTTCGCCGAGCCAGAGGAGACCACCAACAACCCCCGCCTGACCTTCACCCGCCCCCAAAAATCCGGAATTCTGGCGGTCCTACCCGAGCCCGATCGCCCGGCCCAAGTCCTCTACCGCAAGCCCTCACTCTCCAACAACCCCTCCAAGCCAATCCCTTCGATTCCAAGGCCGTCTCTGCAGAGTCAAGCCTCCTCCGACGCCGGCGGTGGCGGTCAGACCCAGTCGGTGCCTTCCAGAAGGTTCCAGCAGCACTCGGCGCCGATGAAGGTGCCGGTGCTGTCCAAGGCGATGATGATGGAGCGGCGCAGGAACCTCGGCGACCTTGCTGACGTGGTGGATGACGACGGCGGAGATGAGGAGATGCTGCCGCCGCATGAGCTGGTGGCGAGGGGGTCGGAGGTGTCGGCGAGGACCACGTTTTCGGTGCTGGAAGGCGTGGGGAGGACTCTCAAAGGTAGGGATTTGCGTCAGGTCAGGAATGCAATTTGGCGCAAGACTGGCTTTCTCGATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044464
KEGG Pathways
Metabolic & Signaling
ko03022 ko05169 ko05203 map03022 map05169 map05203
Pfam Domains
Protein Families
TFIIB TF_Zn_Ribbon

Protein Analysis

216

Amino Acids

23.78

Weight (kDa)

5.82

Isoelectric Point (pI)

59.53

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Senescence_reg PF04520 32 - 216 4.9e-43 Senescence regulator
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014000)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G10330
fragaria_vesca FvH4_6g24330
malus_domestica MD12G1075300.v1.1 MD14G1073800.v1.1
prunus_persica Prupe.7G055400_v2.0.a1
pyrus_communis pycom12g06480 pycom14g06300
rosa_chinensis RchiOBHm_Chr3g0483261
rosa_laevigata RLG00000023294
rosa_multiflora Rmu_sc0007550.1_g000009
rosa_roxburghii Rroxscaffold_6G00398520
rosa_rugosa Rorug03G0205000
rosa_samantha Rh3AG253400 Rh3BG290500 Rh3CG287300 Rh3DG283100
rosa_wichuraiana Rw3G022920

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 3 cut(s) 364, 394, 406
AccBSI CCGCTC 1 cut(s) 439
AccIII TCCGGA 1 cut(s) 200
AcsI RAATTY 1 cut(s) 204
AcyI GRCGYC 2 cut(s) 336, 395
AjiI CACGTC 1 cut(s) 469
AluBI AGCT 3 cut(s) 15, 100, 517
AluI AGCT 3 cut(s) 15, 100, 517
Alw21I GWGCWC 1 cut(s) 102
Alw26I GTCTC 2 cut(s) 149, 315
AlwNI CAGNNNCTG 1 cut(s) 18
Ama87I CYCGRG 1 cut(s) 221
Aor13HI TCCGGA 1 cut(s) 200
AoxI GGCC 4 cut(s) 55, 108, 237, 305
ApeKI GCWGC 3 cut(s) 73, 385, 502
ApoI RAATTY 1 cut(s) 204
ArsI GACNNNNNNTTYG 2 cut(s) 541, 573
AspLEI GCGC 3 cut(s) 397, 444, 630
AspS9I GGNCC 4 cut(s) 43, 214, 238, 549
AsuC2I CCSGG 1 cut(s) 236
AsuHPI GGTGA 1 cut(s) 175
AvaI CYCGRG 1 cut(s) 221
AvaII GGWCC 3 cut(s) 43, 214, 549
BanI GGYRCC 3 cut(s) 364, 394, 406
BanII GRGCYC 2 cut(s) 102, 228
BbsI GAAGAC 1 cut(s) 109
Bbv12I GWGCWC 1 cut(s) 102
BbvI GCAGC 3 cut(s) 60, 397, 489
BccI CCATC 1 cut(s) 427
BceAI ACGGC 3 cut(s) 19, 292, 499
BcgI CGANNNNNNTGC 4 cut(s) 388, 422, 433, 467
BcnI CCSGG 1 cut(s) 236
BcoDI GTCTC 2 cut(s) 149, 315
BfmI CTRYAG 1 cut(s) 314
BfoI RGCGCY 1 cut(s) 398
BisI GCNGC 7 cut(s) 74, 87, 386, 440, 503, 506, 509
BlsI GCNGC 7 cut(s) 75, 88, 387, 441, 504, 507, 510
Bme1390I CCNGG 1 cut(s) 236
Bme18I GGWCC 3 cut(s) 43, 214, 549
BmeT110I CYCGRG 1 cut(s) 221
BmgBI CACGTC 1 cut(s) 469
BmgT120I GGNCC 4 cut(s) 43, 214, 238, 549
BmiI GGNNCC 5 cut(s) 366, 380, 396, 408, 449
BmrFI CCNGG 1 cut(s) 236
BmrI ACTGGG 1 cut(s) 352
BmsI GCATC 1 cut(s) 489
BmuI ACTGGG 1 cut(s) 352
BpiI GAAGAC 1 cut(s) 109
BpuMI CCSGG 1 cut(s) 236
BsaHI GRCGYC 2 cut(s) 336, 395
BsaI GGTCTC 1 cut(s) 149
BsaJI CCNNGG 5 cut(s) 52, 111, 302, 420, 451
BsaWI WCCGGW 1 cut(s) 200
BsaXI ACNNNNNCTCC 2 cut(s) 263, 293
Bse118I RCCGGY 3 cut(s) 89, 338, 409
Bse1I ACTGG 2 cut(s) 358, 640
BseAI TCCGGA 1 cut(s) 200
BseDI CCNNGG 5 cut(s) 52, 111, 302, 420, 451
BseGI GGATG 1 cut(s) 481
BseNI ACTGG 2 cut(s) 358, 640
BseRI GAGGAG 5 cut(s) 70, 110, 167, 319, 509
BseXI GCAGC 3 cut(s) 60, 397, 489
Bsh1285I CGRYCG 3 cut(s) 25, 32, 232
BshFI GGCC 4 cut(s) 57, 110, 239, 307
BshNI GGYRCC 3 cut(s) 364, 394, 406
BsiEI CGRYCG 3 cut(s) 25, 32, 232
BsiHKAI GWGCWC 1 cut(s) 102
BsiHKCI CYCGRG 1 cut(s) 221
BsiSI CCGG 5 cut(s) 90, 201, 236, 339, 410
BsmAI GTCTC 2 cut(s) 149, 315
BsmBI CGTCTC 1 cut(s) 315
BsmI GAATGC 1 cut(s) 622
BsnI GGCC 4 cut(s) 57, 110, 239, 307
Bso31I GGTCTC 1 cut(s) 149
BsoBI CYCGRG 1 cut(s) 221
Bsp1286I GDGCHC 2 cut(s) 102, 228
Bsp13I TCCGGA 1 cut(s) 200
Bsp143I GATC 2 cut(s) 29, 229
BspANI GGCC 4 cut(s) 57, 110, 239, 307
BspEI TCCGGA 1 cut(s) 200
BspLI GGNNCC 5 cut(s) 366, 380, 396, 408, 449
BspMAI CTGCAG 1 cut(s) 318
BspT107I GGYRCC 3 cut(s) 364, 394, 406
BspTNI GGTCTC 1 cut(s) 149
BsrBI CCGCTC 1 cut(s) 439
BsrFI RCCGGY 3 cut(s) 89, 338, 409
BsrI ACTGG 2 cut(s) 358, 640
BssAI RCCGGY 3 cut(s) 89, 338, 409
BssECI CCNNGG 5 cut(s) 52, 111, 302, 420, 451
BssMI GATC 2 cut(s) 29, 229
BssNI GRCGYC 2 cut(s) 336, 395
BssT1I CCWWGG 2 cut(s) 302, 420
Bst6I CTCTTC 1 cut(s) 65
BstACI GRCGYC 2 cut(s) 336, 395
BstC8I GCNNGC 3 cut(s) 91, 257, 340
BstF5I GGATG 1 cut(s) 481
BstH2I RGCGCY 1 cut(s) 398
BstHHI GCGC 3 cut(s) 397, 444, 630
BstKTI GATC 2 cut(s) 32, 232
BstMAI GTCTC 2 cut(s) 149, 315
BstMBI GATC 2 cut(s) 29, 229
BstMCI CGRYCG 3 cut(s) 25, 32, 232
BstMWI GCNNNNNNNGC 6 cut(s) 54, 313, 394, 508, 514, 636
BstSCI CCNGG 1 cut(s) 234
BstSFI CTRYAG 1 cut(s) 314
BstV1I GCAGC 3 cut(s) 60, 397, 489
BstV2I GAAGAC 1 cut(s) 109
BsuRI GGCC 4 cut(s) 57, 110, 239, 307
BtgZI GCGATG 1 cut(s) 440
BtrI CACGTC 1 cut(s) 469
BtsCI GGATG 1 cut(s) 481
Cac8I GCNNGC 3 cut(s) 91, 257, 340
CaiI CAGNNNCTG 1 cut(s) 18
CfoI GCGC 3 cut(s) 397, 444, 630
Cfr10I RCCGGY 3 cut(s) 89, 338, 409
Cfr13I GGNCC 4 cut(s) 43, 214, 238, 549
CpoI CGGWCCG 1 cut(s) 43
CseI GACGC 2 cut(s) 344, 593
CspI CGGWCCG 1 cut(s) 43
CviAII CATG 1 cut(s) 512
DinI GGCGCC 1 cut(s) 396
DpnI GATC 2 cut(s) 31, 231
DpnII GATC 2 cut(s) 29, 229
Eam1104I CTCTTC 1 cut(s) 65
EarI CTCTTC 1 cut(s) 65
EciI GGCGGA 2 cut(s) 73, 501
Ecl136II GAGCTC 1 cut(s) 100
Eco130I CCWWGG 2 cut(s) 302, 420
Eco24I GRGCYC 2 cut(s) 102, 228
Eco31I GGTCTC 1 cut(s) 149
Eco47I GGWCC 3 cut(s) 43, 214, 549
Eco53kI GAGCTC 1 cut(s) 100
Eco88I CYCGRG 1 cut(s) 221
EcoICRI GAGCTC 1 cut(s) 100
EcoRI GAATTC 1 cut(s) 204
EcoT14I CCWWGG 2 cut(s) 302, 420
EcoT38I GRGCYC 2 cut(s) 102, 228
EgeI GGCGCC 1 cut(s) 396
EheI GGCGCC 1 cut(s) 396
ErhI CCWWGG 2 cut(s) 302, 420
Esp3I CGTCTC 1 cut(s) 315
FaeI CATG 1 cut(s) 515
FaiI YATR 1 cut(s) 513
FalI AAGNNNNNCTT 1 cut(s) 623
FatI CATG 1 cut(s) 511
FauI CCCGC 2 cut(s) 179, 194
Fnu4HI GCNGC 7 cut(s) 74, 87, 386, 440, 503, 506, 509
FokI GGATG 1 cut(s) 488
FriOI GRGCYC 2 cut(s) 102, 228
Fsp4HI GCNGC 7 cut(s) 74, 87, 386, 440, 503, 506, 509
GlaI GCGC 3 cut(s) 396, 443, 629
GluI GCNGC 7 cut(s) 74, 87, 386, 440, 503, 506, 509
HaeII RGCGCY 1 cut(s) 398
HaeIII GGCC 4 cut(s) 57, 110, 239, 307
HapII CCGG 5 cut(s) 90, 201, 236, 339, 410
HgaI GACGC 2 cut(s) 344, 593
HhaI GCGC 3 cut(s) 397, 444, 630
Hin1I GRCGYC 2 cut(s) 336, 395
Hin1II CATG 1 cut(s) 515
Hin6I GCGC 3 cut(s) 395, 442, 628
HinP1I GCGC 3 cut(s) 395, 442, 628
HinfI GANTC 4 cut(s) 5, 298, 319, 583
HpaII CCGG 5 cut(s) 90, 201, 236, 339, 410
HphI GGTGA 1 cut(s) 175
Hpy188I TCNGA 4 cut(s) 43, 334, 354, 535
Hpy188III TCNNGA 5 cut(s) 121, 201, 372, 613, 644
Hpy99I CGWCG 5 cut(s) 40, 43, 137, 338, 485
HpyAV CCTTC 7 cut(s) 76, 190, 303, 369, 378, 397, 563
HpyCH4IV ACGT 2 cut(s) 468, 554
HpyCH4V TGCA 2 cut(s) 316, 620
HpyF10VI GCNNNNNNNGC 6 cut(s) 54, 313, 394, 508, 514, 636
HpySE526I ACGT 2 cut(s) 468, 554
Hsp92I GRCGYC 2 cut(s) 336, 395
Hsp92II CATG 1 cut(s) 515
HspAI GCGC 3 cut(s) 395, 442, 628
KasI GGCGCC 1 cut(s) 394
Kpn2I TCCGGA 1 cut(s) 200
KroI GCCGGC 2 cut(s) 89, 338
KroNI GCCGGC 2 cut(s) 91, 340
Kzo9I GATC 2 cut(s) 29, 229
LmnI GCTCC 2 cut(s) 97, 436
Lsp1109I GCAGC 3 cut(s) 60, 397, 489
LweI GCATC 1 cut(s) 489
MaeII ACGT 2 cut(s) 468, 554
MalI GATC 2 cut(s) 31, 231
MbiI CCGCTC 1 cut(s) 439
MboI GATC 2 cut(s) 29, 229
MboII GAAGA 2 cut(s) 52, 109
MhlI GDGCHC 2 cut(s) 102, 228
MluCI AATT 2 cut(s) 204, 621
Mly113I GGCGCC 1 cut(s) 395
MlyI GAGTC 2 cut(s) 328, 577
MmeI TCCRAC 4 cut(s) 21, 294, 357, 513
MreI CGCCGGCG 2 cut(s) 89, 338
MroI TCCGGA 1 cut(s) 200
MroNI GCCGGC 2 cut(s) 89, 338
MspI CCGG 5 cut(s) 90, 201, 236, 339, 410
MspR9I CCNGG 1 cut(s) 236
Mva1269I GAATGC 1 cut(s) 622
MwoI GCNNNNNNNGC 6 cut(s) 54, 313, 394, 508, 514, 636
NaeI GCCGGC 2 cut(s) 91, 340
NarI GGCGCC 1 cut(s) 395
NciI CCSGG 1 cut(s) 236
NdeII GATC 2 cut(s) 29, 229
NgoMIV GCCGGC 2 cut(s) 89, 338
NlaIII CATG 1 cut(s) 515
NlaIV GGNNCC 5 cut(s) 366, 380, 396, 408, 449
NmeAIII GCCGAG 5 cut(s) 33, 136, 169, 371, 432
PcsI WCGNNNNNNNCGW 1 cut(s) 141
PctI GAATGC 1 cut(s) 622
PdiI GCCGGC 2 cut(s) 91, 340
PfeI GAWTC 1 cut(s) 298
PflFI GACNNNGTC 1 cut(s) 358
PkrI GCNGC 7 cut(s) 75, 88, 387, 441, 504, 507, 510
Ple19I CGATCG 2 cut(s) 32, 232
PleI GAGTC 2 cut(s) 327, 577
PluTI GGCGCC 1 cut(s) 398
PpsI GAGTC 2 cut(s) 327, 577
Psp124BI GAGCTC 1 cut(s) 102
PspN4I GGNNCC 5 cut(s) 366, 380, 396, 408, 449
PspPI GGNCC 4 cut(s) 43, 214, 238, 549
PstI CTGCAG 1 cut(s) 318
PstNI CAGNNNCTG 1 cut(s) 18
PsyI GACNNNGTC 1 cut(s) 358
PvuI CGATCG 2 cut(s) 32, 232
Rsr2I CGGWCCG 1 cut(s) 43
RsrII CGGWCCG 1 cut(s) 43
SacI GAGCTC 1 cut(s) 102
SatI GCNGC 7 cut(s) 74, 87, 386, 440, 503, 506, 509
Sau3AI GATC 2 cut(s) 29, 229
Sau96I GGNCC 4 cut(s) 43, 214, 238, 549
SchI GAGTC 2 cut(s) 328, 577
ScrFI CCNGG 1 cut(s) 236
SduI GDGCHC 2 cut(s) 102, 228
SfaNI GCATC 1 cut(s) 489
SfcI CTRYAG 1 cut(s) 314
SfoI GGCGCC 1 cut(s) 396
SgrAI CRCCGGYG 2 cut(s) 89, 338
SinI GGWCC 3 cut(s) 43, 214, 549
Sse9I AATT 2 cut(s) 204, 621
SspDI GGCGCC 1 cut(s) 394
SstI GAGCTC 1 cut(s) 102
StyD4I CCNGG 1 cut(s) 234
StyI CCWWGG 2 cut(s) 302, 420
TaiI ACGT 2 cut(s) 471, 557
TaqI TCGA 2 cut(s) 296, 645
TasI AATT 2 cut(s) 204, 621
TauI GCSGC 4 cut(s) 89, 442, 508, 511
TfiI GAWTC 1 cut(s) 298
TseI GCWGC 3 cut(s) 73, 385, 502
TspDTI ATGAA 1 cut(s) 416
Tth111I GACNNNGTC 1 cut(s) 358
VpaK11BI GGWCC 3 cut(s) 43, 214, 549
XapI RAATTY 1 cut(s) 204
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.