pycom12g06480

Transcription initiation factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr12
OG0014000
Physical Location & Seq
Reverse (-)
6672705 .. 6677228
4524 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom12g06480.6

Sequence Viewer

Length: 939 bp
ATGTCCGATGCCTATTGCTCCGACTGCAAGCGCCAGACGGAGGTCGTGTTCGACCACTCGGCCGGCGACACCGTATGCTCCGAGTGCGGCCTAGTGCTGGAATCCCATTCCATCGACGAGACCTCGGAGTGGCGTACCTTCGCCAACGAGTCGGGCGATAACGACCCGGTCCGTGTTGGCGGCCCGACCAACCCCTTGTTGGCCGATGGCGGGCTGTCCACCGTCATAGCCAAGCCTAATGGGGCTTCTGGTGAGTTCTTGTCTTCGTCCTTGGGTCGGTGGCAGAACCGTGGGTCTAATCCGGATCGGGGCTTGATACTGGCCTTCAAAACCATTGCTACCATGTCTGATAGGTTGGGCCTTGTTGCAACCATCAAGGATCGATCTAATGAGATATATAAAAGGGTGGAAGATCAGAAATCTAGTAGAGGAAGAAATCAGGATGCACTATTGGCTGCTTGCCTGTACATTGCTTGTCGACAAGAAGACAAGCCACGCACTGTAAAGGAAATTTGCTCTGTCGCCAATGGAGCCACAAAGAAGGAAATTGGCCGAGCAAAAGAATACATAGTGAAACAACTGGGATTAGAGAAGGGTCAGTCAGTGGAGATGGGAACAATACATGCTGGGGACTTTATGAGGCGATTCTGTTCCAATCTTGGTATGACTAATCAAGCAGTCAAAGCTGCCCAAGAAGCCGTGACAAAGTCAGAAGAATTTGATATTAGGAGGAGCCCTATATCGATAGCAGCAGCAGTTATTTATATTATCACCCAGCTTTCAGATGACAAGAAGCCTCTCAAAGATATCTCCGTTGCTACAGGAGTAGCAGAAGGAACAATCCGAAACTCATATAAAGACCTTCATCCCCATGTCTCAAAGATTATACCTGGCTGGTACGCAACGGGGGATGCTCTCAAGAGCCTTTGCAGTCCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044464
KEGG Pathways
Metabolic & Signaling
ko03022 ko05169 ko05203 map03022 map05169 map05203
Pfam Domains
Protein Families
TFIIB TF_Zn_Ribbon

Protein Analysis

313

Amino Acids

33.86

Weight (kDa)

6.95

Isoelectric Point (pI)

39.03

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0014000)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G10330
fragaria_vesca FvH4_6g24330
malus_domestica MD12G1075300.v1.1 MD14G1073800.v1.1
prunus_persica Prupe.7G055400_v2.0.a1
pyrus_communis pycom12g06480 pycom14g06300
rosa_chinensis RchiOBHm_Chr3g0483261
rosa_laevigata RLG00000023294
rosa_multiflora Rmu_sc0007550.1_g000009
rosa_roxburghii Rroxscaffold_6G00398520
rosa_rugosa Rorug03G0205000
rosa_samantha Rh3AG253400 Rh3BG290500 Rh3CG287300 Rh3DG283100
rosa_wichuraiana Rw3G022920

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 478
AccIII TCCGGA 1 cut(s) 301
AciI CCGC 3 cut(s) 87, 180, 210
AclWI GGATC 2 cut(s) 312, 387
AcoI YGGCCR 3 cut(s) 60, 201, 550
AcsI RAATTY 2 cut(s) 510, 716
AfaI GTAC 3 cut(s) 136, 467, 899
AfiI CCNNNNNNNGG 7 cut(s) 40, 97, 129, 199, 210, 276, 308
AgsI TTSAA 1 cut(s) 328
AjnI CCWGG 1 cut(s) 889
AloI GAACNNNNNNTCC 2 cut(s) 32, 64
AluBI AGCT 2 cut(s) 686, 778
AluI AGCT 2 cut(s) 686, 778
Alw26I GTCTC 2 cut(s) 113, 880
AlwI GGATC 2 cut(s) 312, 387
Aor13HI TCCGGA 1 cut(s) 301
AoxI GGCC 7 cut(s) 60, 88, 181, 201, 321, 358, 550
ApeKI GCWGC 4 cut(s) 455, 686, 749, 752
ApoI RAATTY 2 cut(s) 510, 716
AspLEI GCGC 1 cut(s) 33
AspS9I GGNCC 3 cut(s) 169, 182, 358
AsuC2I CCSGG 1 cut(s) 167
AsuHPI GGTGA 2 cut(s) 263, 763
AvaII GGWCC 1 cut(s) 169
BanII GRGCYC 1 cut(s) 737
BbsI GAAGAC 2 cut(s) 255, 492
BbvI GCAGC 4 cut(s) 442, 673, 761, 764
BccI CCATC 4 cut(s) 119, 200, 380, 604
BceAI ACGGC 1 cut(s) 683
BciT130I CCWGG 1 cut(s) 891
BcnI CCSGG 1 cut(s) 167
BcoDI GTCTC 2 cut(s) 113, 880
BfaI CTAG 2 cut(s) 92, 423
BfmI CTRYAG 1 cut(s) 819
BfoI RGCGCY 1 cut(s) 34
BisI GCNGC 6 cut(s) 88, 181, 456, 687, 750, 753
BlsI GCNGC 6 cut(s) 89, 182, 457, 688, 751, 754
Bme1390I CCNGG 2 cut(s) 167, 891
Bme18I GGWCC 1 cut(s) 169
BmgT120I GGNCC 3 cut(s) 169, 182, 358
BmiI GGNNCC 2 cut(s) 532, 734
BmrFI CCNGG 2 cut(s) 167, 891
BmrI ACTGGG 1 cut(s) 590
BmsI GCATC 2 cut(s) 433, 901
BmuI ACTGGG 1 cut(s) 590
BoxI GACNNNNGTC 1 cut(s) 41
BpiI GAAGAC 2 cut(s) 255, 492
BpuEI CTTGAG 1 cut(s) 902
BpuMI CCSGG 1 cut(s) 167
Bsa29I ATCGAT 2 cut(s) 382, 743
BsaI GGTCTC 1 cut(s) 113
BsaJI CCNNGG 3 cut(s) 123, 270, 289
BsaWI WCCGGW 1 cut(s) 301
BsaXI ACNNNNNCTCC 2 cut(s) 32, 62
Bsc4I CCNNNNNNNGG 7 cut(s) 40, 97, 129, 199, 210, 276, 308
Bse118I RCCGGY 1 cut(s) 62
Bse1I ACTGG 2 cut(s) 324, 585
Bse3DI GCAATG 2 cut(s) 333, 468
BseAI TCCGGA 1 cut(s) 301
BseBI CCWGG 1 cut(s) 891
BseCI ATCGAT 2 cut(s) 382, 743
BseDI CCNNGG 3 cut(s) 123, 270, 289
BseGI GGATG 3 cut(s) 448, 865, 916
BseLI CCNNNNNNNGG 7 cut(s) 40, 97, 129, 199, 210, 276, 308
BseMI GCAATG 2 cut(s) 333, 468
BseNI ACTGG 2 cut(s) 324, 585
BseRI GAGGAG 1 cut(s) 745
BseX3I CGGCCG 1 cut(s) 60
BseXI GCAGC 4 cut(s) 442, 673, 761, 764
BseYI CCCAGC 2 cut(s) 626, 774
Bsh1285I CGRYCG 1 cut(s) 63
BshFI GGCC 7 cut(s) 62, 90, 183, 203, 323, 360, 552
BshVI ATCGAT 2 cut(s) 382, 743
BsiEI CGRYCG 1 cut(s) 63
BsiSI CCGG 3 cut(s) 63, 167, 302
BslFI GGGAC 1 cut(s) 644
BslI CCNNNNNNNGG 7 cut(s) 40, 97, 129, 199, 210, 276, 308
BsmAI GTCTC 2 cut(s) 113, 880
BsmFI GGGAC 1 cut(s) 644
BsnI GGCC 7 cut(s) 62, 90, 183, 203, 323, 360, 552
Bso31I GGTCTC 1 cut(s) 113
Bsp1286I GDGCHC 1 cut(s) 737
Bsp13I TCCGGA 1 cut(s) 301
Bsp1407I TGTACA 1 cut(s) 465
Bsp143I GATC 4 cut(s) 304, 379, 383, 412
BspACI CCGC 3 cut(s) 87, 180, 210
BspANI GGCC 7 cut(s) 62, 90, 183, 203, 323, 360, 552
BspDI ATCGAT 2 cut(s) 382, 743
BspEI TCCGGA 1 cut(s) 301
BspLI GGNNCC 2 cut(s) 532, 734
BspPI GGATC 2 cut(s) 312, 387
BspTNI GGTCTC 1 cut(s) 113
BsrDI GCAATG 2 cut(s) 333, 468
BsrFI RCCGGY 1 cut(s) 62
BsrGI TGTACA 1 cut(s) 465
BsrI ACTGG 2 cut(s) 324, 585
BssAI RCCGGY 1 cut(s) 62
BssECI CCNNGG 3 cut(s) 123, 270, 289
BssMI GATC 4 cut(s) 304, 379, 383, 412
BssT1I CCWWGG 1 cut(s) 270
Bst2UI CCWGG 1 cut(s) 891
Bst4CI ACNGT 4 cut(s) 73, 223, 290, 502
BstAUI TGTACA 1 cut(s) 465
BstC8I GCNNGC 4 cut(s) 29, 64, 212, 460
BstDSI CCRYGG 1 cut(s) 289
BstF5I GGATG 3 cut(s) 448, 865, 916
BstH2I RGCGCY 1 cut(s) 34
BstHHI GCGC 1 cut(s) 33
BstKTI GATC 4 cut(s) 307, 382, 386, 415
BstMAI GTCTC 2 cut(s) 113, 880
BstMBI GATC 4 cut(s) 304, 379, 383, 412
BstMCI CGRYCG 1 cut(s) 63
BstMWI GCNNNNNNNGC 6 cut(s) 24, 84, 452, 530, 683, 695
BstNI CCWGG 1 cut(s) 891
BstNSI RCATGY 1 cut(s) 626
BstPAI GACNNNNGTC 1 cut(s) 41
BstSCI CCNGG 2 cut(s) 165, 889
BstSFI CTRYAG 1 cut(s) 819
BstV1I GCAGC 4 cut(s) 442, 673, 761, 764
BstV2I GAAGAC 2 cut(s) 255, 492
BstZI CGGCCG 1 cut(s) 60
Bsu15I ATCGAT 2 cut(s) 382, 743
BsuRI GGCC 7 cut(s) 62, 90, 183, 203, 323, 360, 552
BsuTUI ATCGAT 2 cut(s) 382, 743
BtgI CCRYGG 1 cut(s) 289
BtsCI GGATG 3 cut(s) 448, 865, 916
BtsIMutI CAGTG 2 cut(s) 498, 609
Cac8I GCNNGC 4 cut(s) 29, 64, 212, 460
CfoI GCGC 1 cut(s) 33
Cfr10I RCCGGY 1 cut(s) 62
Cfr13I GGNCC 3 cut(s) 169, 182, 358
ClaI ATCGAT 2 cut(s) 382, 743
CpoI CGGWCCG 1 cut(s) 169
Csp6I GTAC 3 cut(s) 135, 466, 898
CspI CGGWCCG 1 cut(s) 169
CviAII CATG 3 cut(s) 343, 623, 872
CviQI GTAC 3 cut(s) 135, 466, 898
DpnI GATC 4 cut(s) 306, 381, 385, 414
DpnII GATC 4 cut(s) 304, 379, 383, 412
EaeI YGGCCR 3 cut(s) 60, 201, 550
EagI CGGCCG 1 cut(s) 60
EclXI CGGCCG 1 cut(s) 60
Eco130I CCWWGG 1 cut(s) 270
Eco24I GRGCYC 1 cut(s) 737
Eco31I GGTCTC 1 cut(s) 113
Eco32I GATATC 1 cut(s) 808
Eco47I GGWCC 1 cut(s) 169
Eco52I CGGCCG 1 cut(s) 60
EcoRII CCWGG 1 cut(s) 889
EcoRV GATATC 1 cut(s) 808
EcoT14I CCWWGG 1 cut(s) 270
EcoT38I GRGCYC 1 cut(s) 737
ErhI CCWWGG 1 cut(s) 270
FaeI CATG 3 cut(s) 346, 626, 875
FaqI GGGAC 1 cut(s) 644
FatI CATG 3 cut(s) 342, 622, 871
FauI CCCGC 1 cut(s) 203
FblI GTMKAC 1 cut(s) 478
Fnu4HI GCNGC 6 cut(s) 88, 181, 456, 687, 750, 753
FokI GGATG 3 cut(s) 455, 852, 923
FriOI GRGCYC 1 cut(s) 737
Fsp4HI GCNGC 6 cut(s) 88, 181, 456, 687, 750, 753
FspBI CTAG 2 cut(s) 92, 423
GlaI GCGC 1 cut(s) 32
GluI GCNGC 6 cut(s) 88, 181, 456, 687, 750, 753
GsaI CCCAGC 2 cut(s) 630, 778
HaeII RGCGCY 1 cut(s) 34
HaeIII GGCC 7 cut(s) 62, 90, 183, 203, 323, 360, 552
HapII CCGG 3 cut(s) 63, 167, 302
HhaI GCGC 1 cut(s) 33
Hin1II CATG 3 cut(s) 346, 626, 875
Hin6I GCGC 1 cut(s) 31
HinP1I GCGC 1 cut(s) 31
HincII GTYRAC 1 cut(s) 479
HindII GTYRAC 1 cut(s) 479
HinfI GANTC 3 cut(s) 101, 149, 645
HpaII CCGG 3 cut(s) 63, 167, 302
HphI GGTGA 2 cut(s) 263, 763
Hpy166II GTNNAC 2 cut(s) 219, 479
Hpy188I TCNGA 9 cut(s) 7, 22, 82, 127, 349, 417, 712, 784, 845
Hpy188III TCNNGA 3 cut(s) 302, 440, 919
Hpy8I GTNNAC 2 cut(s) 219, 479
Hpy99I CGWCG 1 cut(s) 119
HpyAV CCTTC 6 cut(s) 148, 334, 535, 586, 827, 872
HpyCH4III ACNGT 4 cut(s) 73, 223, 290, 502
HpyCH4V TGCA 4 cut(s) 27, 368, 446, 930
HpyF10VI GCNNNNNNNGC 6 cut(s) 24, 84, 452, 530, 683, 695
Hsp92II CATG 3 cut(s) 346, 626, 875
HspAI GCGC 1 cut(s) 31
Kpn2I TCCGGA 1 cut(s) 301
KroI GCCGGC 1 cut(s) 62
KroNI GCCGGC 1 cut(s) 64
Kzo9I GATC 4 cut(s) 304, 379, 383, 412
LmnI GCTCC 4 cut(s) 23, 83, 530, 732
Lsp1109I GCAGC 4 cut(s) 442, 673, 761, 764
LweI GCATC 2 cut(s) 433, 901
MaeI CTAG 2 cut(s) 92, 423
MaeIII GTNAC 1 cut(s) 700
MalI GATC 4 cut(s) 306, 381, 385, 414
MboI GATC 4 cut(s) 304, 379, 383, 412
MboII GAAGA 5 cut(s) 255, 422, 444, 497, 725
MhlI GDGCHC 1 cut(s) 737
MluCI AATT 3 cut(s) 510, 546, 716
MlyI GAGTC 1 cut(s) 158
MmeI TCCRAC 1 cut(s) 45
MnlI CCTC 6 cut(s) 34, 133, 422, 633, 723, 807
MroI TCCGGA 1 cut(s) 301
MroNI GCCGGC 1 cut(s) 62
MslI CAYNNNNRTG 1 cut(s) 870
MspI CCGG 3 cut(s) 63, 167, 302
MspR9I CCNGG 2 cut(s) 167, 891
MvaI CCWGG 1 cut(s) 891
MwoI GCNNNNNNNGC 6 cut(s) 24, 84, 452, 530, 683, 695
NaeI GCCGGC 1 cut(s) 64
NciI CCSGG 1 cut(s) 167
NdeII GATC 4 cut(s) 304, 379, 383, 412
NgoMIV GCCGGC 1 cut(s) 62
NlaIII CATG 3 cut(s) 346, 626, 875
NlaIV GGNNCC 2 cut(s) 532, 734
NmeAIII GCCGAG 2 cut(s) 38, 578
NmuCI GTSAC 1 cut(s) 700
NspI RCATGY 1 cut(s) 626
PcsI WCGNNNNNNNCGW 1 cut(s) 153
PdiI GCCGGC 1 cut(s) 64
PfeI GAWTC 2 cut(s) 101, 645
PflFI GACNNNGTC 2 cut(s) 167, 706
PkrI GCNGC 6 cut(s) 89, 182, 457, 688, 751, 754
PleI GAGTC 1 cut(s) 157
PpsI GAGTC 1 cut(s) 157
PshAI GACNNNNGTC 1 cut(s) 41
Psp6I CCWGG 1 cut(s) 889
PspFI CCCAGC 2 cut(s) 626, 774
PspGI CCWGG 1 cut(s) 889
PspN4I GGNNCC 2 cut(s) 532, 734
PspPI GGNCC 3 cut(s) 169, 182, 358
PsyI GACNNNGTC 2 cut(s) 167, 706
RsaI GTAC 3 cut(s) 136, 467, 899
RsaNI GTAC 3 cut(s) 135, 466, 898
RseI CAYNNNNRTG 1 cut(s) 870
Rsr2I CGGWCCG 1 cut(s) 169
RsrII CGGWCCG 1 cut(s) 169
SalI GTCGAC 1 cut(s) 477
SatI GCNGC 6 cut(s) 88, 181, 456, 687, 750, 753
Sau3AI GATC 4 cut(s) 304, 379, 383, 412
Sau96I GGNCC 3 cut(s) 169, 182, 358
SchI GAGTC 1 cut(s) 158
ScrFI CCNGG 2 cut(s) 167, 891
SduI GDGCHC 1 cut(s) 737
SetI ASST 8 cut(s) 45, 125, 140, 356, 688, 780, 864, 892
SfaNI GCATC 2 cut(s) 433, 901
SfcI CTRYAG 1 cut(s) 819
SinI GGWCC 1 cut(s) 169
SmiMI CAYNNNNRTG 1 cut(s) 870
SmlI CTYRAG 1 cut(s) 917
SmoI CTYRAG 1 cut(s) 917
Sse9I AATT 3 cut(s) 510, 546, 716
SsiI CCGC 3 cut(s) 87, 180, 210
SspMI CTAG 2 cut(s) 92, 423
StyD4I CCNGG 2 cut(s) 165, 889
StyI CCWWGG 1 cut(s) 270
TaaI ACNGT 4 cut(s) 73, 223, 290, 502
TaqI TCGA 5 cut(s) 51, 114, 382, 478, 743
TasI AATT 3 cut(s) 510, 546, 716
TatI WGTACW 1 cut(s) 465
TauI GCSGC 2 cut(s) 90, 183
TfiI GAWTC 2 cut(s) 101, 645
TscAI CASTG 2 cut(s) 505, 609
TseFI GTSAC 1 cut(s) 700
TseI GCWGC 4 cut(s) 455, 686, 749, 752
Tsp45I GTSAC 1 cut(s) 700
TspDTI ATGAA 1 cut(s) 854
TspGWI ACGGA 3 cut(s) 53, 161, 802
TspRI CASTG 2 cut(s) 505, 609
Tth111I GACNNNGTC 2 cut(s) 167, 706
VpaK11BI GGWCC 1 cut(s) 169
XapI RAATTY 2 cut(s) 510, 716
XceI RCATGY 1 cut(s) 626
XcmI CCANNNNNNNNNTGG 1 cut(s) 196
XmiI GTMKAC 1 cut(s) 478
XspI CTAG 2 cut(s) 92, 423
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.