Rh3AG253400

Transcription initiation factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3A
OG0014000
Physical Location & Seq
Forward (+)
27513818 .. 27519653
5836 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3AG253400.1

Sequence Viewer

Length: 1128 bp
ATGCGCGTACGCGTCTCCAACCCAAATAGAGAAAGAGCACGAAACCTTCATGAATGTCCTAACAAACACTGCAATCCCAGGGTCTATAAATACCCTCACCTCCCCCTTCCTATTATCTTAATATTCAACATCTCTCTCAGCTCTCTCTCACCCAAAACCATCCCACCCTCCAAATTTTACAATAAGGAAATGTCCGATGCGTATTGCTCCGACTGCAAGCGCCAGACGGAGGTGGTGTTCGATCACTCCGCCGGCGACACCGTCTGCTCCGAGTGCGGCCTGGTCCTCGAGTCCCATTCCATCGACGAGACGTCCGAGTGGCGAACCTTCGCCAACGAGTCCGGCGATAACGACCCCGTCCGTGTCGGTGGCCCCACCAACCCGCTCTTGGCCGATGGCGGATTGTCCACCGTCATCGCCAAGCCCAATGGAGCTTCCGGCGAGTTCCTCTCTTCGTCTTTGGGCAGGTGGCAGAACCGCGGCTCCAATCCGGATCGGGGCTTGATACTCGCCTTCAAAACCATCGCTACCATGTCCGATAGGTTGGGCCTTGTTGCAACCATCAAGGATCGAGCTAATGAGATATATAAGAGGGTGGAAGATCAAAAGTCTAGTAGAGGAAGAAATCAGGATGCATTATTGGCTGCTTGCCTATACATTGCTTGTCGACAAGAAGACAAGCCCCGCACTGTAAAGGAAATTTGCTCCGTTGCCAATGGAGCCACAAAGAAAGAAATTGGCCGAGCAAAAGAATACATAGTGAAACAGCTGGGATTGGAGAAGGGTCAGTCAGTGGAGATGGGAACAATACATGCCGGGGACTTTATGAGGCGTTTCTGTTCCAACCTTGGTATGAATAATCAAGCGGTCAAAGCTGCTCAAGAAGCTGTGCAGAAGTCGGAAGAATTTGATATAAGGAGGAGCCCTATATCAATAGCAGCAGCTGTTATATATATTATAACTCAACTTTCAGATGATAAGAAGCCTTTAAAAGATATCTCACTCGCTACTGGAGTAGCAGAAGGAACTATCAGAAACTCATACAAAGACCTTTATCCTCATGTATCTAAGATAATACCCAACTGGTATGCCAAGGAGGAGGATCTGAAGAACCTTTGCAGCCCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044464
KEGG Pathways
Metabolic & Signaling
ko03022 ko05169 ko05203 map03022 map05169 map05203
Pfam Domains
Protein Families
TFIIB TF_Zn_Ribbon

Protein Analysis

375

Amino Acids

41.58

Weight (kDa)

8.75

Isoelectric Point (pI)

44.16

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Zn_Ribbon_TF PF08271 68 - 110 2.5e-15 TFIIB zinc-binding
TFIIB PF00382 166 - 248 1.4e-20 Transcription factor TFIIB repeat
TFIIB PF00382 267 - 353 9.1e-14 Transcription factor TFIIB repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014000)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G10330
fragaria_vesca FvH4_6g24330
malus_domestica MD12G1075300.v1.1 MD14G1073800.v1.1
prunus_persica Prupe.7G055400_v2.0.a1
pyrus_communis pycom12g06480 pycom14g06300
rosa_chinensis RchiOBHm_Chr3g0483261
rosa_laevigata RLG00000023294
rosa_multiflora Rmu_sc0007550.1_g000009
rosa_roxburghii Rroxscaffold_6G00398520
rosa_rugosa Rorug03G0205000
rosa_samantha Rh3AG253400 Rh3BG290500 Rh3CG287300 Rh3DG283100
rosa_wichuraiana Rw3G022920

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 959
AarI CACCTGC 1 cut(s) 456
AatII GACGTC 1 cut(s) 314
Acc36I ACCTGC 1 cut(s) 456
AccBSI CCGCTC 1 cut(s) 385
AccI GTMKAC 1 cut(s) 667
AccII CGCG 3 cut(s) 6, 12, 480
AccIII TCCGGA 1 cut(s) 490
AciI CCGC 8 cut(s) 249, 276, 383, 399, 478, 480, 685, 866
AclWI GGATC 3 cut(s) 501, 576, 1110
AcoI YGGCCR 2 cut(s) 390, 739
AcsI RAATTY 3 cut(s) 173, 699, 905
AcuI CTGAAG 1 cut(s) 1127
AcyI GRCGYC 1 cut(s) 311
AfaI GTAC 1 cut(s) 9
AfiI CCNNNNNNNGG 3 cut(s) 229, 388, 497
AflIII ACRYGT 1 cut(s) 10
AgsI TTSAA 2 cut(s) 127, 517
AhdI GACNNNNNGTC 1 cut(s) 310
AjnI CCWGG 2 cut(s) 77, 279
AloI GAACNNNNNNTCC 4 cut(s) 221, 253, 467, 499
AluBI AGCT 7 cut(s) 141, 434, 575, 769, 875, 887, 944
AluI AGCT 7 cut(s) 141, 434, 575, 769, 875, 887, 944
Alw21I GWGCWC 1 cut(s) 40
Alw26I GTCTC 2 cut(s) 19, 302
AlwI GGATC 3 cut(s) 501, 576, 1110
AlwNI CAGNNNCTG 1 cut(s) 944
Ama87I CYCGRG 1 cut(s) 287
Aor13HI TCCGGA 1 cut(s) 490
AoxI GGCC 5 cut(s) 277, 370, 390, 547, 739
ApeKI GCWGC 5 cut(s) 644, 875, 938, 941, 1119
ApoI RAATTY 3 cut(s) 173, 699, 905
AspLEI GCGC 2 cut(s) 6, 222
AspS9I GGNCC 3 cut(s) 283, 371, 547
AsuC2I CCSGG 1 cut(s) 817
AsuHPI GGTGA 2 cut(s) 89, 141
AvaI CYCGRG 1 cut(s) 287
AvaII GGWCC 1 cut(s) 283
BanII GRGCYC 1 cut(s) 926
BbsI GAAGAC 1 cut(s) 681
Bbv12I GWGCWC 1 cut(s) 40
BbvI GCAGC 4 cut(s) 631, 862, 950, 953
BccI CCATC 6 cut(s) 167, 308, 389, 530, 569, 793
BciT130I CCWGG 2 cut(s) 79, 281
BcnI CCSGG 1 cut(s) 817
BcoDI GTCTC 2 cut(s) 19, 302
BfaI CTAG 1 cut(s) 612
BfoI RGCGCY 1 cut(s) 223
BfuAI ACCTGC 1 cut(s) 456
BisI GCNGC 7 cut(s) 277, 481, 645, 876, 939, 942, 1120
BlsI GCNGC 7 cut(s) 278, 482, 646, 877, 940, 943, 1121
Bme1390I CCNGG 3 cut(s) 79, 281, 817
Bme18I GGWCC 1 cut(s) 283
BmeRI GACNNNNNGTC 1 cut(s) 310
BmeT110I CYCGRG 1 cut(s) 287
BmgT120I GGNCC 3 cut(s) 283, 371, 547
BmiI GGNNCC 4 cut(s) 373, 484, 721, 923
BmrFI CCNGG 3 cut(s) 79, 281, 817
BmsI GCATC 2 cut(s) 187, 622
BpiI GAAGAC 1 cut(s) 681
BplI GAGNNNNNCTC 2 cut(s) 434, 466
BpmI CTGGAG 1 cut(s) 1032
BpuEI CTTGAG 1 cut(s) 864
BpuMI CCSGG 1 cut(s) 817
BsaHI GRCGYC 1 cut(s) 311
BsaJI CCNNGG 6 cut(s) 77, 78, 478, 816, 847, 1092
BsaWI WCCGGW 1 cut(s) 490
BsaXI ACNNNNNCTCC 4 cut(s) 221, 251, 467, 497
Bsc4I CCNNNNNNNGG 3 cut(s) 229, 388, 497
Bse118I RCCGGY 1 cut(s) 251
Bse1I ACTGG 2 cut(s) 1015, 1088
Bse3DI GCAATG 1 cut(s) 657
BseAI TCCGGA 1 cut(s) 490
BseBI CCWGG 2 cut(s) 79, 281
BseDI CCNNGG 6 cut(s) 77, 78, 478, 816, 847, 1092
BseGI GGATG 2 cut(s) 159, 637
BseLI CCNNNNNNNGG 3 cut(s) 229, 388, 497
BseMI GCAATG 1 cut(s) 657
BseMII CTCAG 1 cut(s) 151
BseNI ACTGG 2 cut(s) 1015, 1088
BseRI GAGGAG 2 cut(s) 934, 1112
BseXI GCAGC 4 cut(s) 631, 862, 950, 953
BseYI CCCAGC 1 cut(s) 769
BsgI GTGCAG 1 cut(s) 911
Bsh1236I CGCG 3 cut(s) 6, 12, 480
BshFI GGCC 5 cut(s) 279, 372, 392, 549, 741
BsiHKAI GWGCWC 1 cut(s) 40
BsiHKCI CYCGRG 1 cut(s) 287
BsiSI CCGG 5 cut(s) 252, 342, 438, 491, 816
BsiWI CGTACG 1 cut(s) 7
BslFI GGGAC 2 cut(s) 277, 833
BslI CCNNNNNNNGG 3 cut(s) 229, 388, 497
BsmAI GTCTC 2 cut(s) 19, 302
BsmBI CGTCTC 2 cut(s) 19, 302
BsmFI GGGAC 2 cut(s) 277, 833
BsnI GGCC 5 cut(s) 279, 372, 392, 549, 741
BsoBI CYCGRG 1 cut(s) 287
Bsp1286I GDGCHC 2 cut(s) 40, 926
Bsp13I TCCGGA 1 cut(s) 490
Bsp143I GATC 5 cut(s) 241, 493, 568, 601, 1102
BspACI CCGC 8 cut(s) 249, 276, 383, 399, 478, 480, 685, 866
BspANI GGCC 5 cut(s) 279, 372, 392, 549, 741
BspCNI CTCAG 1 cut(s) 150
BspEI TCCGGA 1 cut(s) 490
BspFNI CGCG 3 cut(s) 6, 12, 480
BspHI TCATGA 1 cut(s) 49
BspLI GGNNCC 4 cut(s) 373, 484, 721, 923
BspMI ACCTGC 1 cut(s) 456
BspPI GGATC 3 cut(s) 501, 576, 1110
BsrBI CCGCTC 1 cut(s) 385
BsrDI GCAATG 1 cut(s) 657
BsrFI RCCGGY 1 cut(s) 251
BsrI ACTGG 2 cut(s) 1015, 1088
BssAI RCCGGY 1 cut(s) 251
BssECI CCNNGG 6 cut(s) 77, 78, 478, 816, 847, 1092
BssMI GATC 5 cut(s) 241, 493, 568, 601, 1102
BssNI GRCGYC 1 cut(s) 311
BssT1I CCWWGG 2 cut(s) 847, 1092
Bst2UI CCWGG 2 cut(s) 79, 281
Bst4CI ACNGT 3 cut(s) 262, 412, 691
Bst6I CTCTTC 1 cut(s) 457
BstACI GRCGYC 1 cut(s) 311
BstC8I GCNNGC 3 cut(s) 218, 253, 649
BstDEI CTNAG 2 cut(s) 137, 1068
BstDSI CCRYGG 1 cut(s) 478
BstF5I GGATG 2 cut(s) 159, 637
BstFNI CGCG 3 cut(s) 6, 12, 480
BstH2I RGCGCY 1 cut(s) 223
BstHHI GCGC 2 cut(s) 6, 222
BstKTI GATC 5 cut(s) 244, 496, 571, 604, 1105
BstMAI GTCTC 2 cut(s) 19, 302
BstMBI GATC 5 cut(s) 241, 493, 568, 601, 1102
BstMWI GCNNNNNNNGC 6 cut(s) 213, 273, 641, 719, 872, 884
BstNI CCWGG 2 cut(s) 79, 281
BstNSI RCATGY 1 cut(s) 815
BstSCI CCNGG 3 cut(s) 77, 279, 815
BstUI CGCG 3 cut(s) 6, 12, 480
BstV1I GCAGC 4 cut(s) 631, 862, 950, 953
BstV2I GAAGAC 1 cut(s) 681
BstX2I RGATCY 1 cut(s) 1102
BstYI RGATCY 1 cut(s) 1102
BsuRI GGCC 5 cut(s) 279, 372, 392, 549, 741
BtgI CCRYGG 1 cut(s) 478
BtgZI GCGATG 2 cut(s) 400, 508
BtsCI GGATG 2 cut(s) 159, 637
BtsI GCAGTG 1 cut(s) 67
BtsIMutI CAGTG 3 cut(s) 67, 687, 798
BveI ACCTGC 1 cut(s) 456
Cac8I GCNNGC 3 cut(s) 218, 253, 649
CaiI CAGNNNCTG 1 cut(s) 944
CciI TCATGA 1 cut(s) 49
CfoI GCGC 2 cut(s) 6, 222
Cfr10I RCCGGY 1 cut(s) 251
Cfr13I GGNCC 3 cut(s) 283, 371, 547
Cfr42I CCGCGG 1 cut(s) 481
Csp6I GTAC 1 cut(s) 8
CviAII CATG 4 cut(s) 50, 532, 812, 1061
CviQI GTAC 1 cut(s) 8
DdeI CTNAG 2 cut(s) 137, 1068
DpnI GATC 5 cut(s) 243, 495, 570, 603, 1104
DpnII GATC 5 cut(s) 241, 493, 568, 601, 1102
DraI TTTAAA 1 cut(s) 990
DriI GACNNNNNGTC 1 cut(s) 310
EaeI YGGCCR 2 cut(s) 390, 739
Eam1104I CTCTTC 1 cut(s) 457
Eam1105I GACNNNNNGTC 1 cut(s) 310
EarI CTCTTC 1 cut(s) 457
EciI GGCGGA 2 cut(s) 238, 414
Eco130I CCWWGG 2 cut(s) 847, 1092
Eco24I GRGCYC 1 cut(s) 926
Eco32I GATATC 1 cut(s) 997
Eco47I GGWCC 1 cut(s) 283
Eco57I CTGAAG 1 cut(s) 1127
Eco88I CYCGRG 1 cut(s) 287
EcoRII CCWGG 2 cut(s) 77, 279
EcoRV GATATC 1 cut(s) 997
EcoT14I CCWWGG 2 cut(s) 847, 1092
EcoT22I ATGCAT 1 cut(s) 637
EcoT38I GRGCYC 1 cut(s) 926
ErhI CCWWGG 2 cut(s) 847, 1092
Esp3I CGTCTC 2 cut(s) 19, 302
FaeI CATG 4 cut(s) 53, 535, 815, 1064
FaqI GGGAC 2 cut(s) 277, 833
FatI CATG 4 cut(s) 49, 531, 811, 1060
FauI CCCGC 2 cut(s) 390, 692
FblI GTMKAC 1 cut(s) 667
Fnu4HI GCNGC 7 cut(s) 277, 481, 645, 876, 939, 942, 1120
FokI GGATG 2 cut(s) 146, 644
FriOI GRGCYC 1 cut(s) 926
Fsp4HI GCNGC 7 cut(s) 277, 481, 645, 876, 939, 942, 1120
FspBI CTAG 1 cut(s) 612
GlaI GCGC 2 cut(s) 5, 221
GluI GCNGC 7 cut(s) 277, 481, 645, 876, 939, 942, 1120
GsaI CCCAGC 1 cut(s) 773
GsuI CTGGAG 1 cut(s) 1032
HaeII RGCGCY 1 cut(s) 223
HaeIII GGCC 5 cut(s) 279, 372, 392, 549, 741
HapII CCGG 5 cut(s) 252, 342, 438, 491, 816
HhaI GCGC 2 cut(s) 6, 222
Hin1I GRCGYC 1 cut(s) 311
Hin1II CATG 4 cut(s) 53, 535, 815, 1064
Hin6I GCGC 2 cut(s) 4, 220
HinP1I GCGC 2 cut(s) 4, 220
HincII GTYRAC 1 cut(s) 668
HindII GTYRAC 1 cut(s) 668
HinfI GANTC 2 cut(s) 290, 338
HpaII CCGG 5 cut(s) 252, 342, 438, 491, 816
HphI GGTGA 2 cut(s) 89, 141
Hpy166II GTNNAC 2 cut(s) 408, 668
Hpy188I TCNGA 9 cut(s) 196, 211, 271, 316, 538, 901, 973, 1034, 1107
Hpy188III TCNNGA 4 cut(s) 50, 491, 629, 881
Hpy8I GTNNAC 2 cut(s) 408, 668
Hpy99I CGWCG 1 cut(s) 308
HpyAV CCTTC 6 cut(s) 56, 116, 337, 523, 775, 1016
HpyCH4III ACNGT 3 cut(s) 262, 412, 691
HpyCH4IV ACGT 1 cut(s) 311
HpyCH4V TGCA 6 cut(s) 72, 216, 557, 635, 892, 1119
HpyF10VI GCNNNNNNNGC 6 cut(s) 213, 273, 641, 719, 872, 884
HpyF3I CTNAG 2 cut(s) 137, 1068
HpySE526I ACGT 1 cut(s) 311
Hsp92I GRCGYC 1 cut(s) 311
Hsp92II CATG 4 cut(s) 53, 535, 815, 1064
HspAI GCGC 2 cut(s) 4, 220
Kpn2I TCCGGA 1 cut(s) 490
KroI GCCGGC 1 cut(s) 251
KroNI GCCGGC 1 cut(s) 253
KspI CCGCGG 1 cut(s) 481
Kzo9I GATC 5 cut(s) 241, 493, 568, 601, 1102
LmnI GCTCC 7 cut(s) 212, 272, 431, 488, 710, 719, 921
Lsp1109I GCAGC 4 cut(s) 631, 862, 950, 953
LweI GCATC 2 cut(s) 187, 622
MaeI CTAG 1 cut(s) 612
MaeII ACGT 1 cut(s) 311
MalI GATC 5 cut(s) 243, 495, 570, 603, 1104
MbiI CCGCTC 1 cut(s) 385
MboI GATC 5 cut(s) 241, 493, 568, 601, 1102
MboII GAAGA 6 cut(s) 444, 611, 633, 686, 914, 1120
MflI RGATCY 1 cut(s) 1102
MhlI GDGCHC 2 cut(s) 40, 926
MluCI AATT 4 cut(s) 173, 699, 735, 905
MluI ACGCGT 1 cut(s) 10
MlyI GAGTC 2 cut(s) 299, 347
MmeI TCCRAC 4 cut(s) 42, 234, 867, 879
Mph1103I ATGCAT 1 cut(s) 637
MreI CGCCGGCG 1 cut(s) 251
MroI TCCGGA 1 cut(s) 490
MroNI GCCGGC 1 cut(s) 251
MseI TTAA 2 cut(s) 119, 989
MspA1I CMGCKG 3 cut(s) 480, 769, 944
MspI CCGG 5 cut(s) 252, 342, 438, 491, 816
MspR9I CCNGG 3 cut(s) 79, 281, 817
MvaI CCWGG 2 cut(s) 79, 281
MvnI CGCG 3 cut(s) 6, 12, 480
MwoI GCNNNNNNNGC 6 cut(s) 213, 273, 641, 719, 872, 884
NaeI GCCGGC 1 cut(s) 253
NciI CCSGG 1 cut(s) 817
NdeII GATC 5 cut(s) 241, 493, 568, 601, 1102
NgoMIV GCCGGC 1 cut(s) 251
NlaIII CATG 4 cut(s) 53, 535, 815, 1064
NlaIV GGNNCC 4 cut(s) 373, 484, 721, 923
NmeAIII GCCGAG 1 cut(s) 767
NsiI ATGCAT 1 cut(s) 637
NspI RCATGY 1 cut(s) 815
PaeR7I CTCGAG 1 cut(s) 287
PagI TCATGA 1 cut(s) 49
PaqCI CACCTGC 1 cut(s) 456
PasI CCCWGGG 1 cut(s) 78
PcsI WCGNNNNNNNCGW 2 cut(s) 312, 342
PdiI GCCGGC 1 cut(s) 253
Pfl23II CGTACG 1 cut(s) 7
PflFI GACNNNGTC 2 cut(s) 260, 356
PkrI GCNGC 7 cut(s) 278, 482, 646, 877, 940, 943, 1121
PleI GAGTC 2 cut(s) 298, 346
PpsI GAGTC 2 cut(s) 298, 346
PsiI TTATAA 1 cut(s) 959
Psp6I CCWGG 2 cut(s) 77, 279
PspFI CCCAGC 1 cut(s) 769
PspGI CCWGG 2 cut(s) 77, 279
PspLI CGTACG 1 cut(s) 7
PspN4I GGNNCC 4 cut(s) 373, 484, 721, 923
PspPI GGNCC 3 cut(s) 283, 371, 547
PspXI VCTCGAGB 1 cut(s) 287
PstNI CAGNNNCTG 1 cut(s) 944
PsuI RGATCY 1 cut(s) 1102
PsyI GACNNNGTC 2 cut(s) 260, 356
PvuII CAGCTG 2 cut(s) 769, 944
RsaI GTAC 1 cut(s) 9
RsaNI GTAC 1 cut(s) 8
SacII CCGCGG 1 cut(s) 481
SalI GTCGAC 1 cut(s) 666
SaqAI TTAA 2 cut(s) 119, 989
SatI GCNGC 7 cut(s) 277, 481, 645, 876, 939, 942, 1120
Sau3AI GATC 5 cut(s) 241, 493, 568, 601, 1102
Sau96I GGNCC 3 cut(s) 283, 371, 547
SchI GAGTC 2 cut(s) 299, 347
ScrFI CCNGG 3 cut(s) 79, 281, 817
SduI GDGCHC 2 cut(s) 40, 926
SfaNI GCATC 2 cut(s) 187, 622
Sfr274I CTCGAG 1 cut(s) 287
Sfr303I CCGCGG 1 cut(s) 481
SgrAI CRCCGGYG 1 cut(s) 251
SgrBI CCGCGG 1 cut(s) 481
SinI GGWCC 1 cut(s) 283
SlaI CTCGAG 1 cut(s) 287
SmlI CTYRAG 2 cut(s) 287, 879
SmoI CTYRAG 2 cut(s) 287, 879
Sse9I AATT 4 cut(s) 173, 699, 735, 905
SsiI CCGC 8 cut(s) 249, 276, 383, 399, 478, 480, 685, 866
SspI AATATT 1 cut(s) 123
SspMI CTAG 1 cut(s) 612
StyD4I CCNGG 3 cut(s) 77, 279, 815
StyI CCWWGG 2 cut(s) 847, 1092
TaaI ACNGT 3 cut(s) 262, 412, 691
TaiI ACGT 1 cut(s) 314
TaqI TCGA 5 cut(s) 240, 288, 303, 571, 667
TasI AATT 4 cut(s) 173, 699, 735, 905
TauI GCSGC 2 cut(s) 279, 483
Tru1I TTAA 2 cut(s) 119, 989
Tru9I TTAA 2 cut(s) 119, 989
TscAI CASTG 3 cut(s) 74, 694, 798
TseI GCWGC 5 cut(s) 644, 875, 938, 941, 1119
TspDTI ATGAA 3 cut(s) 38, 66, 869
TspGWI ACGGA 3 cut(s) 242, 350, 697
TspRI CASTG 3 cut(s) 74, 694, 798
Tth111I GACNNNGTC 2 cut(s) 260, 356
VpaK11BI GGWCC 1 cut(s) 283
XapI RAATTY 3 cut(s) 173, 699, 905
XceI RCATGY 1 cut(s) 815
XcmI CCANNNNNNNNNTGG 1 cut(s) 385
XhoI CTCGAG 1 cut(s) 287
XmiI GTMKAC 1 cut(s) 667
XspI CTAG 1 cut(s) 612
ZraI GACGTC 1 cut(s) 312
Zsp2I ATGCAT 1 cut(s) 637
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.