MD14G1073800.v1.1

Transcription initiation factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0014000
Physical Location & Seq
Reverse (-)
8166051 .. 8171667
5617 bp
Loading structure...
UTR
Exon/CDS
Intron
MD14G1073800.v1.1.491

Sequence Viewer

Length: 939 bp
ATGTCCGATGCCTATTGCTCCGACTGCAAGCGCCAGACGGAGGTCGTGTTCGACCACTCGGCGGGTGATACCGTGTGCTCCGAGTGCGGTCTAGTGCTCGAGTCTCATTCCATTGATGAGACCTCGGAGTGGCGTACCTTCGCCAACGAGTCGGGCGATAACGACCCGGTCCGTGTTGGTGGGCCTACCAATCCCCTGTTGGCCGATGGCGGGCTGTCCACCGTCATAGCCAAGCCCAATGGGGCTTCTGGTGAGTTCTTGTCTTCGTCCTTGGGTCGGTGGCAGAACCGTGGGTCTAATCCGGATCGGGGCTTGATAATGGCGTTCAAAACCATCGCTACCATGTCCGATAGGTTGGGCCTTGTTGCAACCATCAAGGATCGGGCTAATGAGATATATAAGAGGGTTGAAGATCAGAAATCTAGTAGGGGAAGAAATCAGGATGCGCTATTGGCTGCTTGCCTGTACATTGCTTGTCGACAAGAAGACAAGCCACGCACTGTAAAGGAAATTTGTTCTGTCGCCAATGGAGCCACAAAGAAGGAAATTGGCCGAGCAAAAGAATACATAGTGAAACAACTGGGATTGGAGAATGGTCAGTCGGTGGAGATGGGAACAATACATGCTGGGGACTTTATGAGGCGATTTTGTTCCAATCTGGGTATGATTAATCAAGCAGTCAAGGCTGCTCATGAAGCTGTGACAAAGTCCGAAGAATTTGATATAAGGCGGAGCCCTATATCGATAGCAGCAGCAGTTATTTATATTATCACCCAGCTTTCAGACGATAAGAAGCCTCTCAAAGATATCTCCGTTGCTACCGGAGTAGCGGAAGGAACAATCAGAAACTCGTACAAAGACCTTTATCCCCATGTGTCAAAGATTATACCGGGCTGGTACGCAACAGAGGATGCTCTCAAGAACCTCTGCAGCCCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044464
KEGG Pathways
Metabolic & Signaling
ko03022 ko05169 ko05203 map03022 map05169 map05203
Pfam Domains
Protein Families
TFIIB TF_Zn_Ribbon

Protein Analysis

313

Amino Acids

34.0

Weight (kDa)

6.26

Isoelectric Point (pI)

38.87

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Zn_Ribbon_TF PF08271 5 - 47 1.9e-15 TFIIB zinc-binding
TFIIB PF00382 103 - 185 5.3e-21 Transcription factor TFIIB repeat
TFIIB PF00382 204 - 290 1.2e-12 Transcription factor TFIIB repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0014000)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G10330
fragaria_vesca FvH4_6g24330
malus_domestica MD12G1075300.v1.1 MD14G1073800.v1.1
prunus_persica Prupe.7G055400_v2.0.a1
pyrus_communis pycom12g06480 pycom14g06300
rosa_chinensis RchiOBHm_Chr3g0483261
rosa_laevigata RLG00000023294
rosa_multiflora Rmu_sc0007550.1_g000009
rosa_roxburghii Rroxscaffold_6G00398520
rosa_rugosa Rorug03G0205000
rosa_samantha Rh3AG253400 Rh3BG290500 Rh3CG287300 Rh3DG283100
rosa_wichuraiana Rw3G022920

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 478
AccIII TCCGGA 1 cut(s) 301
AciI CCGC 5 cut(s) 62, 87, 210, 730, 830
AclWI GGATC 2 cut(s) 312, 387
AcoI YGGCCR 2 cut(s) 201, 550
AcsI RAATTY 2 cut(s) 510, 716
AfaI GTAC 4 cut(s) 136, 467, 854, 899
AfiI CCNNNNNNNGG 6 cut(s) 40, 61, 129, 210, 276, 308
AgsI TTSAA 2 cut(s) 328, 410
AloI GAACNNNNNNTCC 2 cut(s) 32, 64
AluBI AGCT 2 cut(s) 698, 778
AluI AGCT 2 cut(s) 698, 778
Alw21I GWGCWC 2 cut(s) 80, 99
Alw26I GTCTC 2 cut(s) 108, 113
AlwI GGATC 2 cut(s) 312, 387
Ama87I CYCGRG 1 cut(s) 98
Aor13HI TCCGGA 1 cut(s) 301
AoxI GGCC 4 cut(s) 182, 201, 358, 550
ApeKI GCWGC 5 cut(s) 455, 686, 749, 752, 930
ApoI RAATTY 2 cut(s) 510, 716
AseI ATTAAT 1 cut(s) 669
AspLEI GCGC 2 cut(s) 33, 448
AspS9I GGNCC 3 cut(s) 169, 182, 358
AsuC2I CCSGG 2 cut(s) 167, 891
AsuHPI GGTGA 3 cut(s) 77, 263, 763
AvaI CYCGRG 1 cut(s) 98
AvaII GGWCC 1 cut(s) 169
BanII GRGCYC 1 cut(s) 737
BbsI GAAGAC 2 cut(s) 255, 492
Bbv12I GWGCWC 2 cut(s) 80, 99
BbvI GCAGC 4 cut(s) 442, 673, 761, 764
BccI CCATC 4 cut(s) 200, 341, 380, 604
BcnI CCSGG 2 cut(s) 167, 891
BcoDI GTCTC 2 cut(s) 108, 113
BfaI CTAG 2 cut(s) 92, 423
BfmI CTRYAG 1 cut(s) 928
BfoI RGCGCY 1 cut(s) 34
BisI GCNGC 5 cut(s) 456, 687, 750, 753, 931
BlsI GCNGC 5 cut(s) 457, 688, 751, 754, 932
Bme1390I CCNGG 2 cut(s) 167, 891
Bme18I GGWCC 1 cut(s) 169
BmeT110I CYCGRG 1 cut(s) 98
BmgT120I GGNCC 3 cut(s) 169, 182, 358
BmiI GGNNCC 2 cut(s) 532, 734
BmrFI CCNGG 2 cut(s) 167, 891
BmrI ACTGGG 1 cut(s) 590
BmsI GCATC 2 cut(s) 433, 901
BmuI ACTGGG 1 cut(s) 590
BoxI GACNNNNGTC 1 cut(s) 41
BpiI GAAGAC 2 cut(s) 255, 492
BpuEI CTTGAG 1 cut(s) 902
BpuMI CCSGG 2 cut(s) 167, 891
Bsa29I ATCGAT 1 cut(s) 743
BsaI GGTCTC 1 cut(s) 113
BsaJI CCNNGG 3 cut(s) 123, 270, 289
BsaWI WCCGGW 2 cut(s) 301, 821
BsaXI ACNNNNNCTCC 2 cut(s) 32, 62
Bsc4I CCNNNNNNNGG 6 cut(s) 40, 61, 129, 210, 276, 308
Bse1I ACTGG 1 cut(s) 585
Bse3DI GCAATG 1 cut(s) 468
BseAI TCCGGA 1 cut(s) 301
BseCI ATCGAT 1 cut(s) 743
BseDI CCNNGG 3 cut(s) 123, 270, 289
BseGI GGATG 2 cut(s) 448, 916
BseLI CCNNNNNNNGG 6 cut(s) 40, 61, 129, 210, 276, 308
BseMI GCAATG 1 cut(s) 468
BseNI ACTGG 1 cut(s) 585
BseXI GCAGC 4 cut(s) 442, 673, 761, 764
BseYI CCCAGC 2 cut(s) 626, 774
BshFI GGCC 4 cut(s) 184, 203, 360, 552
BshVI ATCGAT 1 cut(s) 743
BsiHKAI GWGCWC 2 cut(s) 80, 99
BsiHKCI CYCGRG 1 cut(s) 98
BsiSI CCGG 4 cut(s) 167, 302, 822, 890
BslFI GGGAC 1 cut(s) 644
BslI CCNNNNNNNGG 6 cut(s) 40, 61, 129, 210, 276, 308
BsmAI GTCTC 2 cut(s) 108, 113
BsmFI GGGAC 1 cut(s) 644
BsnI GGCC 4 cut(s) 184, 203, 360, 552
Bso31I GGTCTC 1 cut(s) 113
BsoBI CYCGRG 1 cut(s) 98
Bsp1286I GDGCHC 3 cut(s) 80, 99, 737
Bsp13I TCCGGA 1 cut(s) 301
Bsp1407I TGTACA 1 cut(s) 465
Bsp143I GATC 3 cut(s) 304, 379, 412
BspACI CCGC 5 cut(s) 62, 87, 210, 730, 830
BspANI GGCC 4 cut(s) 184, 203, 360, 552
BspDI ATCGAT 1 cut(s) 743
BspEI TCCGGA 1 cut(s) 301
BspHI TCATGA 1 cut(s) 691
BspLI GGNNCC 2 cut(s) 532, 734
BspMAI CTGCAG 1 cut(s) 932
BspPI GGATC 2 cut(s) 312, 387
BspTNI GGTCTC 1 cut(s) 113
BsrDI GCAATG 1 cut(s) 468
BsrGI TGTACA 1 cut(s) 465
BsrI ACTGG 1 cut(s) 585
BssECI CCNNGG 3 cut(s) 123, 270, 289
BssMI GATC 3 cut(s) 304, 379, 412
BssT1I CCWWGG 1 cut(s) 270
Bst4CI ACNGT 4 cut(s) 73, 223, 290, 502
BstAUI TGTACA 1 cut(s) 465
BstC8I GCNNGC 3 cut(s) 29, 212, 460
BstDSI CCRYGG 1 cut(s) 289
BstF5I GGATG 2 cut(s) 448, 916
BstH2I RGCGCY 1 cut(s) 34
BstHHI GCGC 2 cut(s) 33, 448
BstKTI GATC 3 cut(s) 307, 382, 415
BstMAI GTCTC 2 cut(s) 108, 113
BstMBI GATC 3 cut(s) 304, 379, 412
BstMWI GCNNNNNNNGC 6 cut(s) 24, 84, 452, 530, 683, 695
BstNSI RCATGY 1 cut(s) 626
BstPAI GACNNNNGTC 1 cut(s) 41
BstSCI CCNGG 2 cut(s) 165, 889
BstSFI CTRYAG 1 cut(s) 928
BstV1I GCAGC 4 cut(s) 442, 673, 761, 764
BstV2I GAAGAC 2 cut(s) 255, 492
Bsu15I ATCGAT 1 cut(s) 743
BsuRI GGCC 4 cut(s) 184, 203, 360, 552
BsuTUI ATCGAT 1 cut(s) 743
BtgI CCRYGG 1 cut(s) 289
BtgZI GCGATG 1 cut(s) 319
BtsCI GGATG 2 cut(s) 448, 916
BtsIMutI CAGTG 1 cut(s) 498
Cac8I GCNNGC 3 cut(s) 29, 212, 460
CciI TCATGA 1 cut(s) 691
CfoI GCGC 2 cut(s) 33, 448
Cfr13I GGNCC 3 cut(s) 169, 182, 358
ClaI ATCGAT 1 cut(s) 743
CpoI CGGWCCG 1 cut(s) 169
Csp6I GTAC 4 cut(s) 135, 466, 853, 898
CspI CGGWCCG 1 cut(s) 169
CviAII CATG 4 cut(s) 343, 623, 692, 872
CviQI GTAC 4 cut(s) 135, 466, 853, 898
DpnI GATC 3 cut(s) 306, 381, 414
DpnII GATC 3 cut(s) 304, 379, 412
EaeI YGGCCR 2 cut(s) 201, 550
EciI GGCGGA 1 cut(s) 745
Eco130I CCWWGG 1 cut(s) 270
Eco24I GRGCYC 1 cut(s) 737
Eco31I GGTCTC 1 cut(s) 113
Eco32I GATATC 1 cut(s) 808
Eco47I GGWCC 1 cut(s) 169
Eco88I CYCGRG 1 cut(s) 98
EcoRV GATATC 1 cut(s) 808
EcoT14I CCWWGG 1 cut(s) 270
EcoT38I GRGCYC 1 cut(s) 737
ErhI CCWWGG 1 cut(s) 270
FaeI CATG 4 cut(s) 346, 626, 695, 875
FaqI GGGAC 1 cut(s) 644
FatI CATG 4 cut(s) 342, 622, 691, 871
FauI CCCGC 2 cut(s) 55, 203
FblI GTMKAC 1 cut(s) 478
Fnu4HI GCNGC 5 cut(s) 456, 687, 750, 753, 931
FokI GGATG 2 cut(s) 455, 923
FriOI GRGCYC 1 cut(s) 737
Fsp4HI GCNGC 5 cut(s) 456, 687, 750, 753, 931
FspBI CTAG 2 cut(s) 92, 423
GlaI GCGC 2 cut(s) 32, 447
GluI GCNGC 5 cut(s) 456, 687, 750, 753, 931
GsaI CCCAGC 2 cut(s) 630, 778
HaeII RGCGCY 1 cut(s) 34
HaeIII GGCC 4 cut(s) 184, 203, 360, 552
HapII CCGG 4 cut(s) 167, 302, 822, 890
HhaI GCGC 2 cut(s) 33, 448
Hin1II CATG 4 cut(s) 346, 626, 695, 875
Hin6I GCGC 2 cut(s) 31, 446
HinP1I GCGC 2 cut(s) 31, 446
HincII GTYRAC 1 cut(s) 479
HindII GTYRAC 1 cut(s) 479
HinfI GANTC 2 cut(s) 101, 149
HpaII CCGG 4 cut(s) 167, 302, 822, 890
HphI GGTGA 3 cut(s) 77, 263, 763
Hpy166II GTNNAC 2 cut(s) 219, 479
Hpy188I TCNGA 9 cut(s) 7, 22, 82, 127, 349, 417, 712, 784, 845
Hpy188III TCNNGA 4 cut(s) 302, 440, 692, 919
Hpy8I GTNNAC 2 cut(s) 219, 479
HpyAV CCTTC 3 cut(s) 148, 535, 827
HpyCH4III ACNGT 4 cut(s) 73, 223, 290, 502
HpyCH4V TGCA 3 cut(s) 27, 368, 930
HpyF10VI GCNNNNNNNGC 6 cut(s) 24, 84, 452, 530, 683, 695
Hsp92II CATG 4 cut(s) 346, 626, 695, 875
HspAI GCGC 2 cut(s) 31, 446
Kpn2I TCCGGA 1 cut(s) 301
Kzo9I GATC 3 cut(s) 304, 379, 412
LmnI GCTCC 4 cut(s) 23, 83, 530, 732
Lsp1109I GCAGC 4 cut(s) 442, 673, 761, 764
LweI GCATC 2 cut(s) 433, 901
MaeI CTAG 2 cut(s) 92, 423
MaeIII GTNAC 1 cut(s) 700
MalI GATC 3 cut(s) 306, 381, 414
MboI GATC 3 cut(s) 304, 379, 412
MboII GAAGA 5 cut(s) 255, 422, 444, 497, 725
MhlI GDGCHC 3 cut(s) 80, 99, 737
MluCI AATT 3 cut(s) 510, 546, 716
MlyI GAGTC 2 cut(s) 110, 158
MmeI TCCRAC 1 cut(s) 45
MnlI CCTC 7 cut(s) 34, 133, 396, 633, 807, 901, 935
MroI TCCGGA 1 cut(s) 301
MseI TTAA 1 cut(s) 669
MspI CCGG 4 cut(s) 167, 302, 822, 890
MspR9I CCNGG 2 cut(s) 167, 891
MwoI GCNNNNNNNGC 6 cut(s) 24, 84, 452, 530, 683, 695
NciI CCSGG 2 cut(s) 167, 891
NdeII GATC 3 cut(s) 304, 379, 412
NlaIII CATG 4 cut(s) 346, 626, 695, 875
NlaIV GGNNCC 2 cut(s) 532, 734
NmeAIII GCCGAG 2 cut(s) 38, 578
NmuCI GTSAC 1 cut(s) 700
NspI RCATGY 1 cut(s) 626
PaeR7I CTCGAG 1 cut(s) 98
PagI TCATGA 1 cut(s) 691
PcsI WCGNNNNNNNCGW 1 cut(s) 153
PflFI GACNNNGTC 2 cut(s) 167, 706
PkrI GCNGC 5 cut(s) 457, 688, 751, 754, 932
PleI GAGTC 2 cut(s) 109, 157
PpsI GAGTC 2 cut(s) 109, 157
PshAI GACNNNNGTC 1 cut(s) 41
PshBI ATTAAT 1 cut(s) 669
PspFI CCCAGC 2 cut(s) 626, 774
PspN4I GGNNCC 2 cut(s) 532, 734
PspPI GGNCC 3 cut(s) 169, 182, 358
PspXI VCTCGAGB 1 cut(s) 98
PstI CTGCAG 1 cut(s) 932
PsyI GACNNNGTC 2 cut(s) 167, 706
RsaI GTAC 4 cut(s) 136, 467, 854, 899
RsaNI GTAC 4 cut(s) 135, 466, 853, 898
Rsr2I CGGWCCG 1 cut(s) 169
RsrII CGGWCCG 1 cut(s) 169
SalI GTCGAC 1 cut(s) 477
SaqAI TTAA 1 cut(s) 669
SatI GCNGC 5 cut(s) 456, 687, 750, 753, 931
Sau3AI GATC 3 cut(s) 304, 379, 412
Sau96I GGNCC 3 cut(s) 169, 182, 358
SchI GAGTC 2 cut(s) 110, 158
ScrFI CCNGG 2 cut(s) 167, 891
SduI GDGCHC 3 cut(s) 80, 99, 737
SetI ASST 8 cut(s) 45, 125, 140, 356, 700, 780, 864, 927
SfaNI GCATC 2 cut(s) 433, 901
SfcI CTRYAG 1 cut(s) 928
Sfr274I CTCGAG 1 cut(s) 98
SinI GGWCC 1 cut(s) 169
SlaI CTCGAG 1 cut(s) 98
SmlI CTYRAG 2 cut(s) 98, 917
SmoI CTYRAG 2 cut(s) 98, 917
Sse9I AATT 3 cut(s) 510, 546, 716
SsiI CCGC 5 cut(s) 62, 87, 210, 730, 830
SspMI CTAG 2 cut(s) 92, 423
StyD4I CCNGG 2 cut(s) 165, 889
StyI CCWWGG 1 cut(s) 270
TaaI ACNGT 4 cut(s) 73, 223, 290, 502
TaqI TCGA 4 cut(s) 51, 99, 478, 743
TasI AATT 3 cut(s) 510, 546, 716
TatI WGTACW 1 cut(s) 465
Tru1I TTAA 1 cut(s) 669
Tru9I TTAA 1 cut(s) 669
TscAI CASTG 1 cut(s) 505
TseFI GTSAC 1 cut(s) 700
TseI GCWGC 5 cut(s) 455, 686, 749, 752, 930
Tsp45I GTSAC 1 cut(s) 700
TspDTI ATGAA 1 cut(s) 708
TspGWI ACGGA 3 cut(s) 53, 161, 802
TspRI CASTG 1 cut(s) 505
Tth111I GACNNNGTC 2 cut(s) 167, 706
VpaK11BI GGWCC 1 cut(s) 169
VspI ATTAAT 1 cut(s) 669
XapI RAATTY 2 cut(s) 510, 716
XceI RCATGY 1 cut(s) 626
XcmI CCANNNNNNNNNTGG 1 cut(s) 196
XhoI CTCGAG 1 cut(s) 98
XmiI GTMKAC 1 cut(s) 478
XspI CTAG 2 cut(s) 92, 423
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.