AT3G14360

Lipase (class 3)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
3
OG0009960
Physical Location & Seq
Forward (+)
4791448 .. 4793684
2237 bp
Loading structure...
UTR
Exon/CDS
Intron
AT3G14360.1

Sequence Viewer

Length: 1557 bp
ATGCACAAAGACAACGATTCGGGTTCGGGTTCGAATCCGGGTCAGGTTTCGAACTACTTGATTGTTCGACCGCACCGAGGTGGGTACATAGACTTGTTCCGGTACGGTGTGAGAGATGACCAAACGAGCAAGGCAAAGTTCTTGGAGATGCCTGATAACCGCGAATGGAGCACGATAACGATTGATGAAGAAGCAGAGGATCACCGTTGGGTAATCGTGGTTTCGATTCTGGTTCGTAAGATTATCAGACTTCTCCGAACACCAATGGAATTCACAGGTTTCGTCGTTGACTTCTTCCTTAATCTGTTCTCTGCGAATGGTGGGTTCTTCGGTTTGCTTCTCCGGTTGATTCAAGCGAAAGTTGTGATACCAGAAAGAGGCTCTGTGACGTTTGTTAGTACAATTGGGCAACTTGATGGGAGAATTAGTCTGTATAAGGAATGGAATTTCGTCGAACACTTGGAGGGCATTGACTCGGTTGATTCTGGAAGGGTTAAAATCGAATTGGGAAGTCGTGGGCTTATGGATTTGTGTGTGATGGCGTCTAAGCTTGCGTATGAAAATGCTAAGGTTGTTGAAAACGTCGTTGATCTTCATTGGAAGATGAATCTGGTGGAGTTCTTGGATTGTTGGAATGATTATCAGAAGCAGATGTCTACACAAGTTTTTGTGTTTACGGATAAGCAGAAAGATGCAAACTTGATAGTAATCAGCTTCAGAGGTACTGAGCCGTTTGATGCTGATGATTGGGGAACAGATTTCGACTATTCGTGGTATGAAGTCCCCAATGTGGGAAAGCTTCACATGGGTTTCCTTGAAGCTATGGGTTTAGGCAACAGAGACGACACTACCACTTTCCATTACAATCTCTTTGAACAAACCTCCTCTGAGGAAGAGAATAGTAAAAAGAATCTTCTGGATATGGTAGAGAGAAGCGCTTATTATGCAGTCCGAGTCATTCTGAAACGTTTGCTTTCGGAACACGAAAACGCTCGGTTTGTAGTCACTGGTCACAGCTTAGGAGGTGCTTTAGCGATACTGTTTCCCACATTGTTGGTGTTGAATGAGGAAACAGAGATAATGAAGAGGCTGCTTGGAGTTTACACGTTTGGACAACCAAGAATCGGGAACAGAGAGGTCGGGTTATTCATGAAAGCTCAACTGAATCAACCTGTGGATCGATACTTCCGAGTCGTTTACTGCAACGATATCGTCCCGAGATTGCCCTACGACGACAAAACGTTCCTCTATAAACATTTCGGACTCTGTCTTTTCTATGACAGCTTCTACAACGAAACAAAAGCAGAGGATGAGCCGGACCCGAACCCTTACGGTCTGCGTTACAAGATTCTTGGGCATGTGATCGCAGTTTGGGAGTTGGTTAGAGGCTTGACGATGGGGTACACTCATGGACCAGATTACAAAGAGGGATGGTTTAGGATCTTGTTTAGGCTTATGGGGCTGGTGATTCCTGGTCTGTCGGATCATTGCATGACTGATTACGTGAACTCGGTTAGGCTTGGACCCGATAATGAACTTCAGATGTCCTCTCTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_3

Protein Analysis

518

Amino Acids

59.61

Weight (kDa)

5.36

Isoelectric Point (pI)

30.44

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_3 PF01764 236 - 411 1.4e-33 Lipase (class 3)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 656
AccII CGCG 1 cut(s) 162
AciI CCGC 2 cut(s) 71, 160
AclI AACGTT 2 cut(s) 967, 1241
AclWI GGATC 4 cut(s) 207, 1185, 1448, 1491
AcsI RAATTY 2 cut(s) 269, 445
AcuI CTGAAG 2 cut(s) 700, 1523
AcyI GRCGYC 1 cut(s) 542
AfaI GTAC 5 cut(s) 86, 104, 400, 724, 1403
AfeI AGCGCT 1 cut(s) 937
AfiI CCNNNNNNNGG 5 cut(s) 77, 377, 790, 791, 1124
AflIII ACRYGT 1 cut(s) 1104
AgsI TTSAA 5 cut(s) 353, 578, 818, 875, 1063
AjnI CCWGG 1 cut(s) 1471
AleI CACNNNNGTG 1 cut(s) 78
AluBI AGCT 7 cut(s) 550, 714, 799, 821, 1017, 1157, 1284
AluI AGCT 7 cut(s) 550, 714, 799, 821, 1017, 1157, 1284
Alw21I GWGCWC 1 cut(s) 173
Alw26I GTCTC 1 cut(s) 834
AlwI GGATC 4 cut(s) 207, 1185, 1448, 1491
Ama87I CYCGRG 1 cut(s) 1216
Aor51HI AGCGCT 1 cut(s) 937
ApeKI GCWGC 1 cut(s) 1090
ApoI RAATTY 2 cut(s) 269, 445
AspLEI GCGC 1 cut(s) 938
AspS9I GGNCC 3 cut(s) 1318, 1412, 1523
AsuC2I CCSGG 1 cut(s) 39
AsuHPI GGTGA 2 cut(s) 194, 1477
AsuII TTCGAA 2 cut(s) 32, 50
AvaI CYCGRG 1 cut(s) 1216
AvaII GGWCC 3 cut(s) 1318, 1412, 1523
Bbv12I GWGCWC 1 cut(s) 173
BbvI GCAGC 1 cut(s) 1077
BccI CCATC 4 cut(s) 410, 532, 1390, 1425
BceAI ACGGC 1 cut(s) 715
BcgI CGANNNNNNTGC 2 cut(s) 1192, 1226
BciT130I CCWGG 1 cut(s) 1473
BcnI CCSGG 1 cut(s) 39
BcoDI GTCTC 1 cut(s) 834
BfoI RGCGCY 1 cut(s) 939
BisI GCNGC 1 cut(s) 1091
BlsI GCNGC 1 cut(s) 1092
Bme1390I CCNGG 2 cut(s) 39, 1473
Bme18I GGWCC 3 cut(s) 1318, 1412, 1523
BmeT110I CYCGRG 1 cut(s) 1216
BmgT120I GGNCC 3 cut(s) 1318, 1412, 1523
BmiI GGNNCC 2 cut(s) 1320, 1525
BmrFI CCNGG 2 cut(s) 39, 1473
BmsI GCATC 3 cut(s) 138, 682, 727
Bpu10I CCTNAGC 2 cut(s) 567, 1018
Bpu14I TTCGAA 2 cut(s) 32, 50
BpuMI CCSGG 1 cut(s) 39
Bsa29I ATCGAT 1 cut(s) 1180
BsaAI YACGTR 1 cut(s) 1504
BsaBI GATNNNNATC 1 cut(s) 707
BsaHI GRCGYC 1 cut(s) 542
BsaJI CCNNGG 1 cut(s) 76
BsaWI WCCGGW 2 cut(s) 99, 342
BsaXI ACNNNNNCTCC 2 cut(s) 412, 442
Bsc4I CCNNNNNNNGG 5 cut(s) 77, 377, 790, 791, 1124
Bse1I ACTGG 1 cut(s) 1012
Bse3DI GCAATG 1 cut(s) 1486
Bse8I GATNNNNATC 1 cut(s) 707
BseBI CCWGG 1 cut(s) 1473
BseCI ATCGAT 1 cut(s) 1180
BseDI CCNNGG 1 cut(s) 76
BseGI GGATG 2 cut(s) 1315, 1436
BseJI GATNNNNATC 1 cut(s) 707
BseLI CCNNNNNNNGG 5 cut(s) 77, 377, 790, 791, 1124
BseMI GCAATG 1 cut(s) 1486
BseMII CTCAG 2 cut(s) 717, 879
BseNI ACTGG 1 cut(s) 1012
BseRI GAGGAG 1 cut(s) 874
BseXI GCAGC 1 cut(s) 1077
Bsh1236I CGCG 1 cut(s) 162
Bsh1285I CGRYCG 1 cut(s) 71
BshVI ATCGAT 1 cut(s) 1180
BsiEI CGRYCG 1 cut(s) 71
BsiHKAI GWGCWC 1 cut(s) 173
BsiHKCI CYCGRG 1 cut(s) 1216
BsiSI CCGG 4 cut(s) 38, 100, 343, 1316
BslFI GGGAC 2 cut(s) 767, 1199
BslI CCNNNNNNNGG 5 cut(s) 77, 377, 790, 791, 1124
BsmAI GTCTC 1 cut(s) 834
BsmBI CGTCTC 1 cut(s) 834
BsmFI GGGAC 2 cut(s) 767, 1199
BsoBI CYCGRG 1 cut(s) 1216
Bsp119I TTCGAA 2 cut(s) 32, 50
Bsp1286I GDGCHC 1 cut(s) 173
Bsp143I GATC 6 cut(s) 199, 589, 1177, 1362, 1440, 1483
BspACI CCGC 2 cut(s) 71, 160
BspCNI CTCAG 2 cut(s) 718, 880
BspDI ATCGAT 1 cut(s) 1180
BspFNI CGCG 1 cut(s) 162
BspHI TCATGA 1 cut(s) 1149
BspLI GGNNCC 2 cut(s) 1320, 1525
BspPI GGATC 4 cut(s) 207, 1185, 1448, 1491
BspT104I TTCGAA 2 cut(s) 32, 50
BsrDI GCAATG 1 cut(s) 1486
BsrI ACTGG 1 cut(s) 1012
BssECI CCNNGG 1 cut(s) 76
BssMI GATC 6 cut(s) 199, 589, 1177, 1362, 1440, 1483
BssNI GRCGYC 1 cut(s) 542
Bst2UI CCWGG 1 cut(s) 1473
Bst4CI ACNGT 4 cut(s) 107, 206, 1041, 1334
Bst6I CTCTTC 2 cut(s) 888, 1079
BstACI GRCGYC 1 cut(s) 542
BstBAI YACGTR 1 cut(s) 1504
BstBI TTCGAA 2 cut(s) 32, 50
BstC8I GCNNGC 1 cut(s) 552
BstDEI CTNAG 5 cut(s) 546, 567, 726, 888, 1018
BstF5I GGATG 2 cut(s) 1315, 1436
BstFNI CGCG 1 cut(s) 162
BstH2I RGCGCY 1 cut(s) 939
BstHHI GCGC 1 cut(s) 938
BstKTI GATC 6 cut(s) 202, 592, 1180, 1365, 1443, 1486
BstMAI GTCTC 1 cut(s) 834
BstMBI GATC 6 cut(s) 199, 589, 1177, 1362, 1440, 1483
BstMCI CGRYCG 1 cut(s) 71
BstMWI GCNNNNNNNGC 3 cut(s) 168, 944, 1459
BstNI CCWGG 1 cut(s) 1473
BstNSI RCATGY 1 cut(s) 1361
BstSCI CCNGG 2 cut(s) 37, 1471
BstUI CGCG 1 cut(s) 162
BstV1I GCAGC 1 cut(s) 1077
BstX2I RGATCY 1 cut(s) 1440
BstXI CCANNNNNNTGG 1 cut(s) 1054
BstYI RGATCY 1 cut(s) 1440
Bsu15I ATCGAT 1 cut(s) 1180
BsuTUI ATCGAT 1 cut(s) 1180
BtsCI GGATG 2 cut(s) 1315, 1436
BtsIMutI CAGTG 1 cut(s) 1005
Cac8I GCNNGC 1 cut(s) 552
CciI TCATGA 1 cut(s) 1149
CfoI GCGC 1 cut(s) 938
Cfr13I GGNCC 3 cut(s) 1318, 1412, 1523
ClaI ATCGAT 1 cut(s) 1180
CseI GACGC 1 cut(s) 531
Csp6I GTAC 5 cut(s) 85, 103, 399, 723, 1402
CviAII CATG 5 cut(s) 805, 1150, 1358, 1409, 1492
CviQI GTAC 5 cut(s) 85, 103, 399, 723, 1402
DdeI CTNAG 5 cut(s) 546, 567, 726, 888, 1018
DpnI GATC 6 cut(s) 201, 591, 1179, 1364, 1442, 1485
DpnII GATC 6 cut(s) 199, 589, 1177, 1362, 1440, 1483
Eam1104I CTCTTC 2 cut(s) 888, 1079
EarI CTCTTC 2 cut(s) 888, 1079
Eco32I GATATC 1 cut(s) 1210
Eco47I GGWCC 3 cut(s) 1318, 1412, 1523
Eco47III AGCGCT 1 cut(s) 937
Eco57I CTGAAG 2 cut(s) 700, 1523
Eco88I CYCGRG 1 cut(s) 1216
EcoRI GAATTC 1 cut(s) 269
EcoRII CCWGG 1 cut(s) 1471
EcoRV GATATC 1 cut(s) 1210
Esp3I CGTCTC 1 cut(s) 834
FaeI CATG 5 cut(s) 808, 1153, 1361, 1412, 1495
FaqI GGGAC 2 cut(s) 767, 1199
FatI CATG 5 cut(s) 804, 1149, 1357, 1408, 1491
FblI GTMKAC 1 cut(s) 656
Fnu4HI GCNGC 1 cut(s) 1091
FokI GGATG 2 cut(s) 1322, 1443
Fsp4HI GCNGC 1 cut(s) 1091
GlaI GCGC 1 cut(s) 937
GluI GCNGC 1 cut(s) 1091
HaeII RGCGCY 1 cut(s) 939
HapII CCGG 4 cut(s) 38, 100, 343, 1316
HgaI GACGC 1 cut(s) 531
HhaI GCGC 1 cut(s) 938
Hin1I GRCGYC 1 cut(s) 542
Hin1II CATG 5 cut(s) 808, 1153, 1361, 1412, 1495
Hin6I GCGC 1 cut(s) 936
HinP1I GCGC 1 cut(s) 936
HincII GTYRAC 1 cut(s) 289
HindII GTYRAC 1 cut(s) 289
HindIII AAGCTT 2 cut(s) 548, 797
HpaII CCGG 4 cut(s) 38, 100, 343, 1316
HphI GGTGA 2 cut(s) 194, 1477
Hpy166II GTNNAC 7 cut(s) 289, 657, 675, 1102, 1198, 1404, 1507
Hpy188III TCNNGA 5 cut(s) 486, 917, 1126, 1150, 1216
Hpy8I GTNNAC 7 cut(s) 289, 657, 675, 1102, 1198, 1404, 1507
Hpy99I CGWCG 4 cut(s) 287, 455, 587, 1235
HpyAV CCTTC 1 cut(s) 483
HpyCH4III ACNGT 4 cut(s) 107, 206, 1041, 1334
HpyCH4IV ACGT 6 cut(s) 389, 582, 967, 1106, 1241, 1503
HpyCH4V TGCA 5 cut(s) 4, 695, 947, 1203, 1491
HpyF10VI GCNNNNNNNGC 3 cut(s) 168, 944, 1459
HpyF3I CTNAG 5 cut(s) 546, 567, 726, 888, 1018
HpySE526I ACGT 6 cut(s) 389, 582, 967, 1106, 1241, 1503
Hsp92I GRCGYC 1 cut(s) 542
Hsp92II CATG 5 cut(s) 808, 1153, 1361, 1412, 1495
HspAI GCGC 1 cut(s) 936
Kzo9I GATC 6 cut(s) 199, 589, 1177, 1362, 1440, 1483
LmnI GCTCC 1 cut(s) 168
Lsp1109I GCAGC 1 cut(s) 1077
LweI GCATC 3 cut(s) 138, 682, 727
MaeII ACGT 6 cut(s) 389, 582, 967, 1106, 1241, 1503
MaeIII GTNAC 4 cut(s) 385, 1003, 1010, 1340
MalI GATC 6 cut(s) 201, 591, 1179, 1364, 1442, 1485
MboI GATC 6 cut(s) 199, 589, 1177, 1362, 1440, 1483
MboII GAAGA 8 cut(s) 200, 286, 319, 584, 613, 905, 905, 1096
MfeI CAATTG 1 cut(s) 402
MflI RGATCY 1 cut(s) 1440
MhlI GDGCHC 1 cut(s) 173
MluCI AATT 5 cut(s) 269, 402, 423, 445, 503
MlyI GAGTC 4 cut(s) 467, 963, 1200, 1257
MmeI TCCRAC 2 cut(s) 611, 1461
MseI TTAA 2 cut(s) 300, 495
MslI CAYNNNNRTG 1 cut(s) 78
MspI CCGG 4 cut(s) 38, 100, 343, 1316
MspR9I CCNGG 2 cut(s) 39, 1473
MunI CAATTG 1 cut(s) 402
MvaI CCWGG 1 cut(s) 1473
MvnI CGCG 1 cut(s) 162
MwoI GCNNNNNNNGC 3 cut(s) 168, 944, 1459
NciI CCSGG 1 cut(s) 39
NdeII GATC 6 cut(s) 199, 589, 1177, 1362, 1440, 1483
NlaIII CATG 5 cut(s) 808, 1153, 1361, 1412, 1495
NlaIV GGNNCC 2 cut(s) 1320, 1525
NmuCI GTSAC 3 cut(s) 385, 1003, 1010
NspI RCATGY 1 cut(s) 1361
NspV TTCGAA 2 cut(s) 32, 50
OliI CACNNNNGTG 1 cut(s) 78
PagI TCATGA 1 cut(s) 1149
PcsI WCGNNNNNNNCGW 2 cut(s) 73, 1186
PflFI GACNNNGTC 1 cut(s) 1266
PkrI GCNGC 1 cut(s) 1092
PleI GAGTC 4 cut(s) 467, 962, 1199, 1257
PpsI GAGTC 4 cut(s) 467, 962, 1199, 1257
Ppu21I YACGTR 1 cut(s) 1504
Psp1406I AACGTT 2 cut(s) 967, 1241
Psp6I CCWGG 1 cut(s) 1471
PspGI CCWGG 1 cut(s) 1471
PspN4I GGNNCC 2 cut(s) 1320, 1525
PspPI GGNCC 3 cut(s) 1318, 1412, 1523
PsuI RGATCY 1 cut(s) 1440
PsyI GACNNNGTC 1 cut(s) 1266
RsaI GTAC 5 cut(s) 86, 104, 400, 724, 1403
RsaNI GTAC 5 cut(s) 85, 103, 399, 723, 1402
RseI CAYNNNNRTG 1 cut(s) 78
SaqAI TTAA 2 cut(s) 300, 495
SatI GCNGC 1 cut(s) 1091
Sau3AI GATC 6 cut(s) 199, 589, 1177, 1362, 1440, 1483
Sau96I GGNCC 3 cut(s) 1318, 1412, 1523
SchI GAGTC 4 cut(s) 467, 963, 1200, 1257
ScrFI CCNGG 2 cut(s) 39, 1473
SduI GDGCHC 1 cut(s) 173
SfaNI GCATC 3 cut(s) 138, 682, 727
SfuI TTCGAA 2 cut(s) 32, 50
SinI GGWCC 3 cut(s) 1318, 1412, 1523
SmiMI CAYNNNNRTG 1 cut(s) 78
Sse9I AATT 5 cut(s) 269, 402, 423, 445, 503
SsiI CCGC 2 cut(s) 71, 160
StyD4I CCNGG 2 cut(s) 37, 1471
TaaI ACNGT 4 cut(s) 107, 206, 1041, 1334
TaiI ACGT 6 cut(s) 392, 585, 970, 1109, 1244, 1506
TaqI TCGA 8 cut(s) 32, 50, 67, 224, 453, 501, 762, 1180
TasI AATT 5 cut(s) 269, 402, 423, 445, 503
TatI WGTACW 1 cut(s) 398
Tru1I TTAA 2 cut(s) 300, 495
Tru9I TTAA 2 cut(s) 300, 495
TscAI CASTG 1 cut(s) 1012
TseFI GTSAC 3 cut(s) 385, 1003, 1010
TseI GCWGC 1 cut(s) 1090
Tsp45I GTSAC 3 cut(s) 385, 1003, 1010
TspDTI ATGAA 9 cut(s) 201, 573, 584, 620, 792, 1097, 1138, 1166, 1548
TspGWI ACGGA 1 cut(s) 692
TspRI CASTG 1 cut(s) 1012
Tth111I GACNNNGTC 1 cut(s) 1266
VpaK11BI GGWCC 3 cut(s) 1318, 1412, 1523
XapI RAATTY 2 cut(s) 269, 445
XceI RCATGY 1 cut(s) 1361
XmiI GTMKAC 1 cut(s) 656
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.