MD09G1171500.v1.1

Lipase (class 3)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr09
OG0009960
Physical Location & Seq
Reverse (-)
14492064 .. 14495283
3220 bp
Loading structure...
UTR
Exon/CDS
Intron
MD09G1171500.v1.1.491

Sequence Viewer

Length: 1671 bp
ATGGAAAAAACCAAGTACCAAGATAATAACCAGAGCCGTTTCGGAGTTGATGATCACGACGGCGGTGATGATGAGTTCAGGTACCTGATCGTACGGCCGGAGAAGGGAGGGGTGAGAGACTTGTTCAGGTACTCGGTGTTGGCGGACGTGGATAGTGGGGCCCGGTTTCTGGAAAGCTCAGACGAAGAGGCGGTGGGTGGAGTTGCAGAGGTTCACAGATGGGTCATTGTGGTGTCGATAATTGCTCGGAAAATAATTGGGTTTTTTGGGAAGCCGATGGAGTGGACTGGCTACGTTGTGGACTTCATCCTCAACCTCTTGTCTCTCAATGGCGGCGTCTTTGGCTTGCTTACTGCACCGCTTCGTGGAACAGTGGTAGTGCCACAGAGGGGCACAGAGACTTTCATAAGCACAACTGGACATTTAGATGGGAGGATAGACCTCCACAGTGGTGAAAACTTAGCAGAAAATGTAGTTGCTTCTCTTTCTGAAGGAAGCGGTATAAAAGCTGAACTGGATAACCGGGGTCTCATGGATCTCTGTATGATGGCTGCCAAGTTAGCGTACGAGAATGCCCAAGTCGTTAGATATATCGTGGTTGATCATTGGAAGGCAAGCTATTCTTGTCCAATGCATTTTGTGGATTTCTACAATTGCTGGAATGATTTCCAAAAGCAGATGTCCACCCAAGTGTTCATACTTTGTGATAAGCCTATAGATGCAAATTTGATATTGATCAGCTTCAGAGGCACAGAACCTTTTGATGCCGATGATTGGAGTACCGATTTTGATTACTCTTGGTATGAAATTCCTAAGTTGGGGAAAGTACACATGGGATTCTTAGAAGCCTTAGGCTTGGGCGATAGAACGAATGCTGCAACCTTCTACAATCAACTTATAGCGATACCCAACAAATTCACTCCTGAAAATGGTGTTGGTGTTAGCAAAAGACCTTCCGAAGGTACCCAAGCATTGTCCTCAGACATCATGGAACAAGGTGGACGTGATCAGTACGACAAGGTAGTTCCACCGGACATGGTGAAGAAGTCTGCATACTATGCTGTAAAAAGAAAGCTCAATAGCTTACTAGAGGAGCACAAAAATGCAAAATTCATGGTTACTGGACATAGCCTAGGCGGCGCGCTTGCCATACTGTTCCCATCAGTGCTGGTGTTGCATGAGGAGATGGAGTTGATGCAGAGGTTGCTTGGTGTGTACACATTTGGGCAGCCCCGGGTTGGTAATAGGGAACTGGGGAGGTACATGGAAGCCCATTTGAATAATCCGGTGCCAAAGTACTTCAGGGTGGTCTACTGCAACGATCTTGTGCCCCGGTTGCCTTACGATGACAAAACTTTCTTGTTTAAGCACTTTGGGGTGTGCCTTTACTATGACAGCCTATACAATGAACAAAGAGTGGAGGAGGAGCCAAACCGTAACTACTTTGGAATGAGATATCTGATAGCAGCATATCTCAATGCTGTTTGGGAGCTAATAAGAGGCTTAACAATGGGCTACACACATGGACCTGAGTACAGGGAGGGTTGGTTCTCCATTTTGCTAAGGATCATAGGACTTGTAGCTCCTGGTTTCTCTGCACATTCCCCCACAAATTACATCAATTCCGTGAGGCTCGGAAAGGACCGCGTTATTCAGATGCTCTCTTTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_3

Protein Analysis

557

Amino Acids

62.98

Weight (kDa)

5.68

Isoelectric Point (pI)

28.87

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_3 PF01764 246 - 449 4.9e-34 Lipase (class 3)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 2 cut(s) 81, 962
AccB1I GGYRCC 3 cut(s) 81, 962, 1288
AccI GTMKAC 1 cut(s) 1311
AccII CGCG 2 cut(s) 1142, 1647
AciI CCGC 8 cut(s) 63, 143, 191, 333, 359, 498, 1137, 1645
AclWI GGATC 2 cut(s) 543, 1574
AcoI YGGCCR 1 cut(s) 95
AcsI RAATTY 4 cut(s) 724, 807, 914, 1109
AcuI CTGAAG 3 cut(s) 510, 727, 1285
AcyI GRCGYC 1 cut(s) 336
AfiI CCNNNNNNNGG 9 cut(s) 104, 169, 365, 389, 774, 818, 929, 959, 1238
AgsI TTSAA 1 cut(s) 1279
AjiI CACGTC 2 cut(s) 148, 1004
AjnI CCWGG 1 cut(s) 1585
AjuI GAANNNNNNNTTGG 2 cut(s) 918, 950
AleI CACNNNNGTG 2 cut(s) 450, 689
AloI GAACNNNNNNTCC 2 cut(s) 1532, 1564
AluBI AGCT 8 cut(s) 177, 509, 618, 741, 1075, 1083, 1492, 1583
AluI AGCT 8 cut(s) 177, 509, 618, 741, 1075, 1083, 1492, 1583
Alw21I GWGCWC 1 cut(s) 1098
Alw26I GTCTC 4 cut(s) 111, 327, 392, 533
AlwI GGATC 2 cut(s) 543, 1574
Ama87I CYCGRG 1 cut(s) 1233
AoxI GGCC 2 cut(s) 95, 159
ApaI GGGCCC 1 cut(s) 163
ApeKI GCWGC 4 cut(s) 551, 875, 1228, 1466
ApoI RAATTY 4 cut(s) 724, 807, 914, 1109
Asp700I GAANNNNTTC 1 cut(s) 665
Asp718I GGTACC 2 cut(s) 81, 962
AspA2I CCTAGG 1 cut(s) 1132
AspLEI GCGC 2 cut(s) 1142, 1144
AspS9I GGNCC 4 cut(s) 159, 160, 1526, 1642
AsuC2I CCSGG 5 cut(s) 163, 524, 1234, 1235, 1333
AsuHPI GGTGA 4 cut(s) 77, 124, 464, 1051
AvaI CYCGRG 1 cut(s) 1233
AvaII GGWCC 2 cut(s) 1526, 1642
AvrII CCTAGG 1 cut(s) 1132
AxyI CCTNAGG 1 cut(s) 850
BaeGI GKGCMC 3 cut(s) 163, 395, 1332
BanI GGYRCC 3 cut(s) 81, 962, 1288
BanII GRGCYC 1 cut(s) 163
BarI GAAGNNNNNNTAC 2 cut(s) 1037, 1069
Bbv12I GWGCWC 1 cut(s) 1098
BbvI GCAGC 4 cut(s) 538, 862, 1240, 1478
BccI CCATC 6 cut(s) 213, 271, 422, 541, 1168, 1180
BceAI ACGGC 3 cut(s) 21, 76, 110
BciT130I CCWGG 1 cut(s) 1587
BclI TGATCA 4 cut(s) 52, 601, 735, 1006
BcnI CCSGG 5 cut(s) 163, 524, 1234, 1235, 1333
BcoDI GTCTC 4 cut(s) 111, 327, 392, 533
BfaI CTAG 2 cut(s) 1088, 1133
BfmI CTRYAG 1 cut(s) 714
BglI GCCNNNNNGGC 1 cut(s) 1137
BisI GCNGC 6 cut(s) 334, 552, 876, 1138, 1229, 1467
BlnI CCTAGG 1 cut(s) 1132
BlsI GCNGC 6 cut(s) 335, 553, 877, 1139, 1230, 1468
BmcAI AGTACT 1 cut(s) 1298
Bme1390I CCNGG 6 cut(s) 163, 524, 1234, 1235, 1333, 1587
Bme18I GGWCC 2 cut(s) 1526, 1642
BmeT110I CYCGRG 1 cut(s) 1233
BmgBI CACGTC 2 cut(s) 148, 1004
BmgT120I GGNCC 4 cut(s) 159, 160, 1526, 1642
BmiI GGNNCC 6 cut(s) 83, 160, 161, 964, 1290, 1428
BmrFI CCNGG 6 cut(s) 163, 524, 1234, 1235, 1333, 1587
BmrI ACTGGG 1 cut(s) 1262
BmsI GCATC 4 cut(s) 709, 754, 1185, 1647
BmuI ACTGGG 1 cut(s) 1262
Bpu10I CCTNAGC 1 cut(s) 1562
BpuMI CCSGG 5 cut(s) 163, 524, 1234, 1235, 1333
BsaBI GATNNNNATC 1 cut(s) 734
BsaHI GRCGYC 1 cut(s) 336
BsaI GGTCTC 1 cut(s) 533
BsaJI CCNNGG 5 cut(s) 523, 1132, 1232, 1233, 1331
BsaWI WCCGGW 2 cut(s) 1030, 1285
BsaXI ACNNNNNCTCC 4 cut(s) 1175, 1205, 1532, 1562
Bsc4I CCNNNNNNNGG 9 cut(s) 104, 169, 365, 389, 774, 818, 929, 959, 1238
Bse1I ACTGG 5 cut(s) 292, 421, 519, 1126, 1257
Bse21I CCTNAGG 1 cut(s) 850
Bse8I GATNNNNATC 1 cut(s) 734
BseBI CCWGG 1 cut(s) 1587
BseDI CCNNGG 5 cut(s) 523, 1132, 1232, 1233, 1331
BseGI GGATG 1 cut(s) 306
BseJI GATNNNNATC 1 cut(s) 734
BseLI CCNNNNNNNGG 9 cut(s) 104, 169, 365, 389, 774, 818, 929, 959, 1238
BseMII CTCAG 3 cut(s) 192, 993, 1521
BseNI ACTGG 5 cut(s) 292, 421, 519, 1126, 1257
BsePI GCGCGC 1 cut(s) 1140
BseRI GAGGAG 4 cut(s) 1106, 1196, 1436, 1439
BseSI GKGCMC 3 cut(s) 163, 395, 1332
BseX3I CGGCCG 1 cut(s) 95
BseXI GCAGC 4 cut(s) 538, 862, 1240, 1478
BsgI GTGCAG 2 cut(s) 339, 1581
Bsh1236I CGCG 2 cut(s) 1142, 1647
Bsh1285I CGRYCG 1 cut(s) 98
BshFI GGCC 2 cut(s) 97, 161
BshNI GGYRCC 3 cut(s) 81, 962, 1288
BsiEI CGRYCG 1 cut(s) 98
BsiHKAI GWGCWC 1 cut(s) 1098
BsiHKCI CYCGRG 1 cut(s) 1233
BsiSI CCGG 7 cut(s) 98, 163, 523, 1031, 1234, 1286, 1333
BsiWI CGTACG 2 cut(s) 91, 564
BslI CCNNNNNNNGG 9 cut(s) 104, 169, 365, 389, 774, 818, 929, 959, 1238
BsmAI GTCTC 4 cut(s) 111, 327, 392, 533
BsmI GAATGC 2 cut(s) 577, 877
BsnI GGCC 2 cut(s) 97, 161
Bso31I GGTCTC 1 cut(s) 533
BsoBI CYCGRG 1 cut(s) 1233
Bsp120I GGGCCC 1 cut(s) 159
Bsp1286I GDGCHC 4 cut(s) 163, 395, 1098, 1332
Bsp1407I TGTACA 1 cut(s) 1215
Bsp143I GATC 8 cut(s) 52, 87, 535, 601, 735, 1006, 1321, 1566
BspACI CCGC 8 cut(s) 63, 143, 191, 333, 359, 498, 1137, 1645
BspANI GGCC 2 cut(s) 97, 161
BspCNI CTCAG 3 cut(s) 191, 992, 1522
BspFNI CGCG 2 cut(s) 1142, 1647
BspLI GGNNCC 6 cut(s) 83, 160, 161, 964, 1290, 1428
BspPI GGATC 2 cut(s) 543, 1574
BspT107I GGYRCC 3 cut(s) 81, 962, 1288
BspTNI GGTCTC 1 cut(s) 533
BsrGI TGTACA 1 cut(s) 1215
BsrI ACTGG 5 cut(s) 292, 421, 519, 1126, 1257
BssECI CCNNGG 5 cut(s) 523, 1132, 1232, 1233, 1331
BssHII GCGCGC 1 cut(s) 1140
BssMI GATC 8 cut(s) 52, 87, 535, 601, 735, 1006, 1321, 1566
BssNI GRCGYC 1 cut(s) 336
BssT1I CCWWGG 1 cut(s) 1132
Bst2UI CCWGG 1 cut(s) 1587
Bst4CI ACNGT 4 cut(s) 373, 449, 1155, 1436
Bst6I CTCTTC 1 cut(s) 180
BstACI GRCGYC 1 cut(s) 336
BstAPI GCANNNNNTGC 2 cut(s) 1058, 1204
BstAUI TGTACA 1 cut(s) 1215
BstC8I GCNNGC 4 cut(s) 347, 616, 1142, 1146
BstDEI CTNAG 8 cut(s) 178, 460, 813, 841, 850, 979, 1530, 1562
BstENI CCTNNNNNAGG 1 cut(s) 957
BstF5I GGATG 1 cut(s) 306
BstFNI CGCG 2 cut(s) 1142, 1647
BstHHI GCGC 2 cut(s) 1142, 1144
BstKTI GATC 8 cut(s) 55, 90, 538, 604, 738, 1009, 1324, 1569
BstMAI GTCTC 4 cut(s) 111, 327, 392, 533
BstMBI GATC 8 cut(s) 52, 87, 535, 601, 735, 1006, 1321, 1566
BstMCI CGRYCG 1 cut(s) 98
BstMWI GCNNNNNNNGC 8 cut(s) 342, 560, 747, 1058, 1137, 1174, 1204, 1336
BstNI CCWGG 1 cut(s) 1587
BstSCI CCNGG 6 cut(s) 161, 522, 1232, 1233, 1331, 1585
BstSFI CTRYAG 1 cut(s) 714
BstSLI GKGCMC 3 cut(s) 163, 395, 1332
BstUI CGCG 2 cut(s) 1142, 1647
BstV1I GCAGC 4 cut(s) 538, 862, 1240, 1478
BstX2I RGATCY 1 cut(s) 535
BstYI RGATCY 1 cut(s) 535
BstZI CGGCCG 1 cut(s) 95
Bsu36I CCTNAGG 1 cut(s) 850
BsuRI GGCC 2 cut(s) 97, 161
BtrI CACGTC 2 cut(s) 148, 1004
BtsCI GGATG 1 cut(s) 306
BtsIMutI CAGTG 3 cut(s) 378, 454, 1170
Cac8I GCNNGC 4 cut(s) 347, 616, 1142, 1146
CfoI GCGC 2 cut(s) 1142, 1144
Cfr13I GGNCC 4 cut(s) 159, 160, 1526, 1642
Cfr9I CCCGGG 1 cut(s) 1233
CseI GACGC 1 cut(s) 325
CviAII CATG 8 cut(s) 532, 832, 988, 1036, 1114, 1178, 1264, 1523
DdeI CTNAG 8 cut(s) 178, 460, 813, 841, 850, 979, 1530, 1562
DpnI GATC 8 cut(s) 54, 89, 537, 603, 737, 1008, 1323, 1568
DpnII GATC 8 cut(s) 52, 87, 535, 601, 735, 1006, 1321, 1566
EaeI YGGCCR 1 cut(s) 95
EagI CGGCCG 1 cut(s) 95
Eam1104I CTCTTC 1 cut(s) 180
EarI CTCTTC 1 cut(s) 180
EciI GGCGGA 1 cut(s) 158
EclXI CGGCCG 1 cut(s) 95
Eco130I CCWWGG 1 cut(s) 1132
Eco24I GRGCYC 1 cut(s) 163
Eco31I GGTCTC 1 cut(s) 533
Eco32I GATATC 1 cut(s) 1457
Eco47I GGWCC 2 cut(s) 1526, 1642
Eco52I CGGCCG 1 cut(s) 95
Eco57I CTGAAG 3 cut(s) 510, 727, 1285
Eco81I CCTNAGG 1 cut(s) 850
Eco88I CYCGRG 1 cut(s) 1233
EcoNI CCTNNNNNAGG 1 cut(s) 957
EcoO109I RGGNCCY 1 cut(s) 159
EcoRII CCWGG 1 cut(s) 1585
EcoRV GATATC 1 cut(s) 1457
EcoT14I CCWWGG 1 cut(s) 1132
EcoT22I ATGCAT 1 cut(s) 636
EcoT38I GRGCYC 1 cut(s) 163
ErhI CCWWGG 1 cut(s) 1132
FaeI CATG 8 cut(s) 535, 835, 991, 1039, 1117, 1181, 1267, 1526
FalI AAGNNNNNCTT 2 cut(s) 607, 639
FatI CATG 8 cut(s) 531, 831, 987, 1035, 1113, 1177, 1263, 1522
FbaI TGATCA 4 cut(s) 52, 601, 735, 1006
FblI GTMKAC 1 cut(s) 1311
Fnu4HI GCNGC 6 cut(s) 334, 552, 876, 1138, 1229, 1467
FokI GGATG 1 cut(s) 293
FriOI GRGCYC 1 cut(s) 163
Fsp4HI GCNGC 6 cut(s) 334, 552, 876, 1138, 1229, 1467
FspBI CTAG 2 cut(s) 1088, 1133
GlaI GCGC 2 cut(s) 1141, 1143
GluI GCNGC 6 cut(s) 334, 552, 876, 1138, 1229, 1467
HaeIII GGCC 2 cut(s) 97, 161
HapII CCGG 7 cut(s) 98, 163, 523, 1031, 1234, 1286, 1333
HgaI GACGC 1 cut(s) 325
HhaI GCGC 2 cut(s) 1142, 1144
Hin1I GRCGYC 1 cut(s) 336
Hin1II CATG 8 cut(s) 535, 835, 991, 1039, 1117, 1181, 1267, 1526
Hin6I GCGC 2 cut(s) 1140, 1142
HinP1I GCGC 2 cut(s) 1140, 1142
HinfI GANTC 1 cut(s) 837
HpaII CCGG 7 cut(s) 98, 163, 523, 1031, 1234, 1286, 1333
HphI GGTGA 4 cut(s) 77, 124, 464, 1051
Hpy166II GTNNAC 9 cut(s) 214, 285, 301, 684, 829, 1001, 1216, 1218, 1312
Hpy188III TCNNGA 3 cut(s) 56, 170, 923
Hpy8I GTNNAC 9 cut(s) 214, 285, 301, 684, 829, 1001, 1216, 1218, 1312
Hpy99I CGWCG 1 cut(s) 62
HpyAV CCTTC 6 cut(s) 97, 485, 604, 892, 953, 963
HpyCH4III ACNGT 4 cut(s) 373, 449, 1155, 1436
HpyCH4IV ACGT 3 cut(s) 147, 294, 1003
HpyF10VI GCNNNNNNNGC 8 cut(s) 342, 560, 747, 1058, 1137, 1174, 1204, 1336
HpyF3I CTNAG 8 cut(s) 178, 460, 813, 841, 850, 979, 1530, 1562
HpySE526I ACGT 3 cut(s) 147, 294, 1003
Hsp92I GRCGYC 1 cut(s) 336
Hsp92II CATG 8 cut(s) 535, 835, 991, 1039, 1117, 1181, 1267, 1526
HspAI GCGC 2 cut(s) 1140, 1142
KpnI GGTACC 2 cut(s) 85, 966
Ksp22I TGATCA 4 cut(s) 52, 601, 735, 1006
Kzo9I GATC 8 cut(s) 52, 87, 535, 601, 735, 1006, 1321, 1566
LmnI GCTCC 4 cut(s) 1093, 1426, 1489, 1588
Lsp1109I GCAGC 4 cut(s) 538, 862, 1240, 1478
LweI GCATC 4 cut(s) 709, 754, 1185, 1647
MaeI CTAG 2 cut(s) 1088, 1133
MaeII ACGT 3 cut(s) 147, 294, 1003
MaeIII GTNAC 2 cut(s) 1117, 1436
MalI GATC 8 cut(s) 54, 89, 537, 603, 737, 1008, 1323, 1568
MboI GATC 8 cut(s) 52, 87, 535, 601, 735, 1006, 1321, 1566
MboII GAAGA 2 cut(s) 197, 1054
MfeI CAATTG 1 cut(s) 652
MflI RGATCY 1 cut(s) 535
MhlI GDGCHC 4 cut(s) 163, 395, 1098, 1332
MluCI AATT 9 cut(s) 240, 255, 652, 724, 807, 914, 1109, 1612, 1621
Mph1103I ATGCAT 1 cut(s) 636
MroXI GAANNNNTTC 1 cut(s) 665
MseI TTAA 3 cut(s) 1365, 1505, 1669
MslI CAYNNNNRTG 5 cut(s) 230, 426, 450, 689, 1101
MspI CCGG 7 cut(s) 98, 163, 523, 1031, 1234, 1286, 1333
MspR9I CCNGG 6 cut(s) 163, 524, 1234, 1235, 1333, 1587
MunI CAATTG 1 cut(s) 652
Mva1269I GAATGC 2 cut(s) 577, 877
MvaI CCWGG 1 cut(s) 1587
MvnI CGCG 2 cut(s) 1142, 1647
MwoI GCNNNNNNNGC 8 cut(s) 342, 560, 747, 1058, 1137, 1174, 1204, 1336
NciI CCSGG 5 cut(s) 163, 524, 1234, 1235, 1333
NdeII GATC 8 cut(s) 52, 87, 535, 601, 735, 1006, 1321, 1566
NlaIII CATG 8 cut(s) 535, 835, 991, 1039, 1117, 1181, 1267, 1526
NlaIV GGNNCC 6 cut(s) 83, 160, 161, 964, 1290, 1428
NsiI ATGCAT 1 cut(s) 636
OliI CACNNNNGTG 2 cut(s) 450, 689
PauI GCGCGC 1 cut(s) 1140
PctI GAATGC 2 cut(s) 577, 877
PdmI GAANNNNTTC 1 cut(s) 665
PfeI GAWTC 1 cut(s) 837
Pfl23II CGTACG 2 cut(s) 91, 564
PkrI GCNGC 6 cut(s) 335, 553, 877, 1139, 1230, 1468
Psp6I CCWGG 1 cut(s) 1585
PspGI CCWGG 1 cut(s) 1585
PspLI CGTACG 2 cut(s) 91, 564
PspN4I GGNNCC 6 cut(s) 83, 160, 161, 964, 1290, 1428
PspOMI GGGCCC 1 cut(s) 159
PspPI GGNCC 4 cut(s) 159, 160, 1526, 1642
PsuI RGATCY 1 cut(s) 535
PteI GCGCGC 1 cut(s) 1140
RseI CAYNNNNRTG 5 cut(s) 230, 426, 450, 689, 1101
SaqAI TTAA 3 cut(s) 1365, 1505, 1669
SatI GCNGC 6 cut(s) 334, 552, 876, 1138, 1229, 1467
Sau3AI GATC 8 cut(s) 52, 87, 535, 601, 735, 1006, 1321, 1566
Sau96I GGNCC 4 cut(s) 159, 160, 1526, 1642
ScaI AGTACT 1 cut(s) 1298
ScrFI CCNGG 6 cut(s) 163, 524, 1234, 1235, 1333, 1587
SduI GDGCHC 4 cut(s) 163, 395, 1098, 1332
SfaNI GCATC 4 cut(s) 709, 754, 1185, 1647
SfcI CTRYAG 1 cut(s) 714
SinI GGWCC 2 cut(s) 1526, 1642
SmaI CCCGGG 1 cut(s) 1235
SmiMI CAYNNNNRTG 5 cut(s) 230, 426, 450, 689, 1101
Sse9I AATT 9 cut(s) 240, 255, 652, 724, 807, 914, 1109, 1612, 1621
SsiI CCGC 8 cut(s) 63, 143, 191, 333, 359, 498, 1137, 1645
SspMI CTAG 2 cut(s) 1088, 1133
StyD4I CCNGG 6 cut(s) 161, 522, 1232, 1233, 1331, 1585
StyI CCWWGG 1 cut(s) 1132
TaaI ACNGT 4 cut(s) 373, 449, 1155, 1436
TaiI ACGT 3 cut(s) 150, 297, 1006
TaqI TCGA 1 cut(s) 236
TasI AATT 9 cut(s) 240, 255, 652, 724, 807, 914, 1109, 1612, 1621
TatI WGTACW 4 cut(s) 826, 1215, 1296, 1533
TauI GCSGC 2 cut(s) 336, 1140
TfiI GAWTC 1 cut(s) 837
Tru1I TTAA 3 cut(s) 1365, 1505, 1669
Tru9I TTAA 3 cut(s) 1365, 1505, 1669
TscAI CASTG 3 cut(s) 378, 454, 1170
TseI GCWGC 4 cut(s) 551, 875, 1228, 1466
TspDTI ATGAA 6 cut(s) 295, 394, 685, 819, 1102, 1422
TspGWI ACGGA 1 cut(s) 1615
TspMI CCCGGG 1 cut(s) 1233
TspRI CASTG 3 cut(s) 378, 454, 1170
VpaK11BI GGWCC 2 cut(s) 1526, 1642
XagI CCTNNNNNAGG 1 cut(s) 957
XapI RAATTY 4 cut(s) 724, 807, 914, 1109
XmaI CCCGGG 1 cut(s) 1233
XmaJI CCTAGG 1 cut(s) 1132
XmiI GTMKAC 1 cut(s) 1311
XmnI GAANNNNTTC 1 cut(s) 665
XspI CTAG 2 cut(s) 1088, 1133
ZrmI AGTACT 1 cut(s) 1298
Zsp2I ATGCAT 1 cut(s) 636
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.