FvH4_6g36190

Lipase (class 3)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0009960
Physical Location & Seq
Forward (+)
28467348 .. 28470444
3097 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g36190.t3

Sequence Viewer

Length: 1056 bp
ATGAGAATGCCAAGGTCATTAGAGGGATCGTCACTGACCATTGGAAGATGCATTTTGTGGACTTTTATGACTGCTGGAATGATGTCCACTCAAGTGTTCATACTTTGTGACAAGCCTAAAGATGCAAATTTTATAATGGTAAGTTTCCGGGGGACAGAACCTTTTGATGCCGATGATTGGAGTACTGATTTTGACTACTCTTGGTATGAAATTCCACAATTGGGTAAAGTACACATGGGATTCTTGGAAGCCATAGGCTTGGGCAATAGAACTGATGTTGCAACCTTCTACAATCAAATTCTTGTATACAATCAACTTCAAGAGAAACACAAGTTCACCCCTCAACAAGGTGTTGAGGGTAGCGAAAGGCCTTCAGATGGTATAACAGCACTATCCTCCAATGGCTTCAAGATCGTTCCACCAGATATGGTAAAGAAGAGTGCATACTATGTTGTAAGAGAAAAGCTCAAGAGCTTACTAGAGGAGCACAAAAATGCCAAATTCATTGTTACAGGACATAGTCTAGGTGGGGCGCTCGCGATCTTGTTCCCTTGTGTGCTGGTGTTGCATGAGGAGATGGAGTTGATGGATAGGTTGTTAGGTGTAAACACATTTGGACAGCCAAGGATAGGGAATAGGCAGCTTGGGAGGTACATGGAATCTTATCTGAATAATCCGGTCCGAAAGTACTTCAGGGTTGTCTACTCCAATGATCTTGTCCCCAGGTTGCCTTACGATAGCAGAACCTTCTTGTTTAAACATTTCGGGGTCTGCCTTTACTACGACAGCCTATACAAAGAACATGTAACTAAGGAGGAGCCAAATAAAAATTTCCTTGGCCTGGGATATCTGATGCCATCGTATCTGACTGCTGCTTGGGAGCTACTAAGAAGTTTTATAATGGGGTACACATATGGACCAGAGTACAAAGAAGGGTGGTTTTCCGTTCTTCTAAGGATCGTAGGACTTGTTCTTCCTGGATTCTCTGCTCATTCCCCTACAAATTATGTGAACTCGGTGAGGCTTGGAAAGGAACGAATTGTCCAAAGTTATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_3

Protein Analysis

352

Amino Acids

40.25

Weight (kDa)

8.47

Isoelectric Point (pI)

37.67

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_3 PF01764 126 - 325 3.5e-32 Lipase (class 3)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 134, 899
AccI GTMKAC 2 cut(s) 306, 702
AccII CGCG 1 cut(s) 539
AclWI GGATC 2 cut(s) 34, 965
AcsI RAATTY 5 cut(s) 127, 210, 297, 500, 829
AcuI CTGAAG 2 cut(s) 357, 676
AfaI GTAC 6 cut(s) 184, 231, 653, 689, 908, 926
AfiI CCNNNNNNNGG 6 cut(s) 177, 221, 347, 377, 629, 841
AflIII ACRYGT 1 cut(s) 802
AgsI TTSAA 2 cut(s) 320, 409
AjnI CCWGG 3 cut(s) 722, 840, 976
AleI CACNNNNGTG 1 cut(s) 92
AloI GAACNNNNNNTCC 1 cut(s) 1026
AluBI AGCT 4 cut(s) 466, 474, 643, 883
AluI AGCT 4 cut(s) 466, 474, 643, 883
Alw21I GWGCWC 1 cut(s) 489
AlwI GGATC 2 cut(s) 34, 965
AoxI GGCC 2 cut(s) 368, 838
ApeKI GCWGC 2 cut(s) 640, 872
ApoI RAATTY 5 cut(s) 127, 210, 297, 500, 829
AspLEI GCGC 1 cut(s) 535
AspS9I GGNCC 2 cut(s) 679, 917
AsuC2I CCSGG 1 cut(s) 149
AsuHPI GGTGA 2 cut(s) 328, 1030
AvaII GGWCC 2 cut(s) 679, 917
BarI GAAGNNNNNNTAC 2 cut(s) 428, 460
Bbv12I GWGCWC 1 cut(s) 489
BbvI GCAGC 2 cut(s) 652, 859
BccI CCATC 4 cut(s) 371, 571, 580, 865
BciT130I CCWGG 3 cut(s) 724, 842, 978
BcnI CCSGG 1 cut(s) 149
BfaI CTAG 2 cut(s) 479, 524
BfoI RGCGCY 1 cut(s) 536
BisI GCNGC 2 cut(s) 641, 873
BlsI GCNGC 2 cut(s) 642, 874
BmcAI AGTACT 2 cut(s) 184, 689
Bme1390I CCNGG 4 cut(s) 149, 724, 842, 978
Bme18I GGWCC 2 cut(s) 679, 917
BmgT120I GGNCC 2 cut(s) 679, 917
BmiI GGNNCC 1 cut(s) 819
BmrFI CCNGG 4 cut(s) 149, 724, 842, 978
BmsI GCATC 4 cut(s) 38, 112, 157, 843
BplI GAGNNNNNCTC 2 cut(s) 450, 482
BpuEI CTTGAG 2 cut(s) 75, 452
BpuMI CCSGG 1 cut(s) 149
BsaJI CCNNGG 6 cut(s) 11, 148, 623, 722, 835, 841
BsaWI WCCGGW 1 cut(s) 676
BsaXI ACNNNNNCTCC 2 cut(s) 566, 596
Bsc4I CCNNNNNNNGG 6 cut(s) 177, 221, 347, 377, 629, 841
BseBI CCWGG 3 cut(s) 724, 842, 978
BseDI CCNNGG 6 cut(s) 11, 148, 623, 722, 835, 841
BseLI CCNNNNNNNGG 6 cut(s) 177, 221, 347, 377, 629, 841
BseRI GAGGAG 3 cut(s) 497, 587, 830
BseXI GCAGC 2 cut(s) 652, 859
Bsh1236I CGCG 1 cut(s) 539
BshFI GGCC 2 cut(s) 370, 840
BsiHKAI GWGCWC 1 cut(s) 489
BsiSI CCGG 2 cut(s) 148, 677
BslFI GGGAC 2 cut(s) 166, 704
BslI CCNNNNNNNGG 6 cut(s) 177, 221, 347, 377, 629, 841
BsmFI GGGAC 2 cut(s) 166, 704
BsmI GAATGC 1 cut(s) 12
BsnI GGCC 2 cut(s) 370, 840
Bsp1286I GDGCHC 1 cut(s) 489
Bsp143I GATC 5 cut(s) 26, 411, 540, 712, 957
Bsp68I TCGCGA 1 cut(s) 539
BspANI GGCC 2 cut(s) 370, 840
BspFNI CGCG 1 cut(s) 539
BspLI GGNNCC 1 cut(s) 819
BspPI GGATC 2 cut(s) 34, 965
BssECI CCNNGG 6 cut(s) 11, 148, 623, 722, 835, 841
BssMI GATC 5 cut(s) 26, 411, 540, 712, 957
BssNAI GTATAC 1 cut(s) 307
BssT1I CCWWGG 3 cut(s) 11, 623, 835
Bst1107I GTATAC 1 cut(s) 307
Bst2UI CCWGG 3 cut(s) 724, 842, 978
Bst6I CTCTTC 1 cut(s) 431
BstC8I GCNNGC 1 cut(s) 537
BstDEI CTNAG 3 cut(s) 810, 887, 953
BstENI CCTNNNNNAGG 1 cut(s) 345
BstFNI CGCG 1 cut(s) 539
BstH2I RGCGCY 1 cut(s) 536
BstHHI GCGC 1 cut(s) 535
BstKTI GATC 5 cut(s) 29, 414, 543, 715, 960
BstMBI GATC 5 cut(s) 26, 411, 540, 712, 957
BstMWI GCNNNNNNNGC 1 cut(s) 565
BstNI CCWGG 3 cut(s) 724, 842, 978
BstNSI RCATGY 1 cut(s) 806
BstSCI CCNGG 4 cut(s) 147, 722, 840, 976
BstUI CGCG 1 cut(s) 539
BstV1I GCAGC 2 cut(s) 652, 859
BstXI CCANNNNNNTGG 1 cut(s) 259
BstZ17I GTATAC 1 cut(s) 307
BsuRI GGCC 2 cut(s) 370, 840
BtsIMutI CAGTG 1 cut(s) 32
BtuMI TCGCGA 1 cut(s) 539
Cac8I GCNNGC 1 cut(s) 537
CfoI GCGC 1 cut(s) 535
Cfr13I GGNCC 2 cut(s) 679, 917
CpoI CGGWCCG 1 cut(s) 679
Csp6I GTAC 6 cut(s) 183, 230, 652, 688, 907, 925
CspI CGGWCCG 1 cut(s) 679
CviAII CATG 4 cut(s) 235, 569, 655, 803
CviQI GTAC 6 cut(s) 183, 230, 652, 688, 907, 925
DdeI CTNAG 3 cut(s) 810, 887, 953
DpnI GATC 5 cut(s) 28, 413, 542, 714, 959
DpnII GATC 5 cut(s) 26, 411, 540, 712, 957
DraI TTTAAA 1 cut(s) 757
Eam1104I CTCTTC 1 cut(s) 431
EarI CTCTTC 1 cut(s) 431
Eco130I CCWWGG 3 cut(s) 11, 623, 835
Eco147I AGGCCT 1 cut(s) 370
Eco32I GATATC 1 cut(s) 848
Eco47I GGWCC 2 cut(s) 679, 917
Eco57I CTGAAG 2 cut(s) 357, 676
EcoNI CCTNNNNNAGG 1 cut(s) 345
EcoRII CCWGG 3 cut(s) 722, 840, 976
EcoRV GATATC 1 cut(s) 848
EcoT14I CCWWGG 3 cut(s) 11, 623, 835
EcoT22I ATGCAT 1 cut(s) 53
ErhI CCWWGG 3 cut(s) 11, 623, 835
FaeI CATG 4 cut(s) 238, 572, 658, 806
FaqI GGGAC 2 cut(s) 166, 704
FatI CATG 4 cut(s) 234, 568, 654, 802
FauNDI CATATG 1 cut(s) 913
FblI GTMKAC 2 cut(s) 306, 702
Fnu4HI GCNGC 2 cut(s) 641, 873
Fsp4HI GCNGC 2 cut(s) 641, 873
FspBI CTAG 2 cut(s) 479, 524
GlaI GCGC 1 cut(s) 534
GluI GCNGC 2 cut(s) 641, 873
HaeII RGCGCY 1 cut(s) 536
HaeIII GGCC 2 cut(s) 370, 840
HapII CCGG 2 cut(s) 148, 677
HhaI GCGC 1 cut(s) 535
Hin1II CATG 4 cut(s) 238, 572, 658, 806
Hin6I GCGC 1 cut(s) 533
HinP1I GCGC 1 cut(s) 533
HinfI GANTC 3 cut(s) 240, 659, 981
HpaII CCGG 2 cut(s) 148, 677
HphI GGTGA 2 cut(s) 328, 1030
Hpy166II GTNNAC 9 cut(s) 60, 87, 232, 307, 336, 607, 703, 909, 1012
Hpy188I TCNGA 5 cut(s) 376, 669, 683, 852, 867
Hpy188III TCNNGA 4 cut(s) 320, 409, 469, 538
Hpy8I GTNNAC 9 cut(s) 60, 87, 232, 307, 336, 607, 703, 909, 1012
HpyAV CCTTC 4 cut(s) 295, 381, 757, 926
HpyCH4V TGCA 5 cut(s) 51, 125, 281, 443, 568
HpyF10VI GCNNNNNNNGC 1 cut(s) 565
HpyF3I CTNAG 3 cut(s) 810, 887, 953
Hsp92II CATG 4 cut(s) 238, 572, 658, 806
HspAI GCGC 1 cut(s) 533
Kzo9I GATC 5 cut(s) 26, 411, 540, 712, 957
LmnI GCTCC 3 cut(s) 484, 817, 880
Lsp1109I GCAGC 2 cut(s) 652, 859
LweI GCATC 4 cut(s) 38, 112, 157, 843
MaeI CTAG 2 cut(s) 479, 524
MaeIII GTNAC 4 cut(s) 30, 107, 508, 805
MalI GATC 5 cut(s) 28, 413, 542, 714, 959
MboI GATC 5 cut(s) 26, 411, 540, 712, 957
MboII GAAGA 4 cut(s) 57, 448, 941, 965
MfeI CAATTG 1 cut(s) 218
MhlI GDGCHC 1 cut(s) 489
MluCI AATT 8 cut(s) 127, 210, 218, 297, 500, 829, 1003, 1038
MnlI CCTC 9 cut(s) 16, 349, 351, 406, 475, 565, 642, 808, 1014
Mph1103I ATGCAT 1 cut(s) 53
MseI TTAA 1 cut(s) 756
MslI CAYNNNNRTG 2 cut(s) 92, 492
MspI CCGG 2 cut(s) 148, 677
MspR9I CCNGG 4 cut(s) 149, 724, 842, 978
MssI GTTTAAAC 1 cut(s) 757
MunI CAATTG 1 cut(s) 218
Mva1269I GAATGC 1 cut(s) 12
MvaI CCWGG 3 cut(s) 724, 842, 978
MvnI CGCG 1 cut(s) 539
MwoI GCNNNNNNNGC 1 cut(s) 565
NciI CCSGG 1 cut(s) 149
NdeI CATATG 1 cut(s) 913
NdeII GATC 5 cut(s) 26, 411, 540, 712, 957
NlaIII CATG 4 cut(s) 238, 572, 658, 806
NlaIV GGNNCC 1 cut(s) 819
NmuCI GTSAC 2 cut(s) 30, 107
NruI TCGCGA 1 cut(s) 539
NsiI ATGCAT 1 cut(s) 53
NspI RCATGY 1 cut(s) 806
OliI CACNNNNGTG 1 cut(s) 92
PceI AGGCCT 1 cut(s) 370
PciI ACATGT 1 cut(s) 802
PctI GAATGC 1 cut(s) 12
PfeI GAWTC 3 cut(s) 240, 659, 981
PflFI GACNNNGTC 1 cut(s) 519
PfoI TCCNGGA 1 cut(s) 976
PkrI GCNGC 2 cut(s) 642, 874
PmeI GTTTAAAC 1 cut(s) 757
PscI ACATGT 1 cut(s) 802
PsiI TTATAA 2 cut(s) 134, 899
Psp6I CCWGG 3 cut(s) 722, 840, 976
PspGI CCWGG 3 cut(s) 722, 840, 976
PspN4I GGNNCC 1 cut(s) 819
PspPI GGNCC 2 cut(s) 679, 917
PsrI GAACNNNNNNTAC 2 cut(s) 954, 986
PsyI GACNNNGTC 1 cut(s) 519
RruI TCGCGA 1 cut(s) 539
RsaI GTAC 6 cut(s) 184, 231, 653, 689, 908, 926
RsaNI GTAC 6 cut(s) 183, 230, 652, 688, 907, 925
RseI CAYNNNNRTG 2 cut(s) 92, 492
Rsr2I CGGWCCG 1 cut(s) 679
RsrII CGGWCCG 1 cut(s) 679
SaqAI TTAA 1 cut(s) 756
SatI GCNGC 2 cut(s) 641, 873
Sau3AI GATC 5 cut(s) 26, 411, 540, 712, 957
Sau96I GGNCC 2 cut(s) 679, 917
ScaI AGTACT 2 cut(s) 184, 689
ScrFI CCNGG 4 cut(s) 149, 724, 842, 978
SduI GDGCHC 1 cut(s) 489
SfaNI GCATC 4 cut(s) 38, 112, 157, 843
SinI GGWCC 2 cut(s) 679, 917
SmiMI CAYNNNNRTG 2 cut(s) 92, 492
SmlI CTYRAG 2 cut(s) 90, 467
SmoI CTYRAG 2 cut(s) 90, 467
Sse9I AATT 8 cut(s) 127, 210, 218, 297, 500, 829, 1003, 1038
SseBI AGGCCT 1 cut(s) 370
SspMI CTAG 2 cut(s) 479, 524
StuI AGGCCT 1 cut(s) 370
StyD4I CCNGG 4 cut(s) 147, 722, 840, 976
StyI CCWWGG 3 cut(s) 11, 623, 835
TasI AATT 8 cut(s) 127, 210, 218, 297, 500, 829, 1003, 1038
TatI WGTACW 4 cut(s) 182, 229, 687, 924
TfiI GAWTC 3 cut(s) 240, 659, 981
Tru1I TTAA 1 cut(s) 756
Tru9I TTAA 1 cut(s) 756
TscAI CASTG 1 cut(s) 39
TseFI GTSAC 2 cut(s) 30, 107
TseI GCWGC 2 cut(s) 640, 872
Tsp45I GTSAC 2 cut(s) 30, 107
TspDTI ATGAA 3 cut(s) 88, 222, 493
TspGWI ACGGA 1 cut(s) 934
TspRI CASTG 1 cut(s) 39
Tth111I GACNNNGTC 1 cut(s) 519
VpaK11BI GGWCC 2 cut(s) 679, 917
XagI CCTNNNNNAGG 1 cut(s) 345
XapI RAATTY 5 cut(s) 127, 210, 297, 500, 829
XceI RCATGY 1 cut(s) 806
XmiI GTMKAC 2 cut(s) 306, 702
XspI CTAG 2 cut(s) 479, 524
ZrmI AGTACT 2 cut(s) 184, 689
Zsp2I ATGCAT 1 cut(s) 53
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.