Rh2BG475800

Lipase (class 3)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2B
OG0009960
Physical Location & Seq
Forward (+)
67098699 .. 67102208
3510 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2BG475800.1

Sequence Viewer

Length: 1293 bp
ATGCTAATTGGGGAAGCAGGGACGGTGGAGGTGCCGCAGAGGGGGACGGAGACTTTCATAAGTACAATTGGTCATTTAGATGGGCGGATAAATCTGTACAAGAAGGGTGAAAGATTAGCGGAAGACATTGTTGCTTTTGTTTATTCTGAAGAAACAAGTACAAAGGCAGAACTAGGGAACAGGGCTCTTATGGACCTCTGTATCATGGCTTCCAAGTTAGCATATGAGAATGCCGAAGTCGTTAGAGAAATCGTCATTGACCATTGGAAGATGCATTTTGTGGACTTTTATGATTGCTGGAATGATTTCCAAAAGCAGATGTCCACCCAAGTGTTCATACTTTGTGACAAGCCTAAAGATGCAAATTTCGTATTGGTAAGTTTCCGGGGGACAGAACCTTTTGATGCTGATGATTGGAGTACTGATTTTGACTACTCTTGGTATGAAATTCCAGAATTGGGTAAAGTACACATGGGATTCTTGGAAGCCTTAGGCTTGGGCAACAGAACTGATGTTGTAAACTTCTACAATCAAATTTTTGTATACAATCAACTTCAAGAGAAACACAAGCTCACCCCTCAAAAAGGTGTTGATGGTAGCAAAAGGCCTTCAGAAGGTATTAAAGCACTATCCTCTAATGGCATCAAGATAGTTCCACCAGATATGGTAGAGAAGAGTGCATACTATGCTGTAAGAAAAAAGCTCAAGAGCTTACTAGAGGAGCACAAAAACGCAAAATTCGTTGTTACAGGGCATAGTCTAGGTGGGGCACTTGCGATATTGTTCCCTTGTGTGCTGGTGTTGCATGAGGAGATGGAGTTAATGGATAGGCTGTTGGGTATATATACATTTGGACAGCCAAGGATAGGGAATAGGCAGCTGGGGAGGTACATGGAAGTCCATCTGAATAAGCCGGTCCAAAAGTACTTCAGGGTTGTCTACTCCAACGATCTTGTCCCCAGGTTGCCTTATGATGACAGAACCTTCTTGTTTAAACATTTCGGTGTCTGCCTTTACTACGACAGCCTATACAATGAACATGTAACTAAGGAGGAGCCAAACAGAAATTTCCTTGGACTGAGATTTCTAATACCATTGTATCTGACTGCCGCTTGGGAGCTACTAAGAAGTTTTACAATGGGGTACGTATATGGACCAGAGTACAAAGAAGGGTGGTTTTCCATTCTGCTAAGGATGGTAGGACTTGTTCTTCCTGGTTTCGCTGCACATTCCCCTACAAATTATGTTAACTCGGTGAGGCTTGGAAAGGAACGGCTCGTCCAAAGTTATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_3

Protein Analysis

430

Amino Acids

49.5

Weight (kDa)

6.33

Isoelectric Point (pI)

32.61

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_3 PF01764 126 - 325 1.2e-33 Lipase (class 3)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 31
AccI GTMKAC 2 cut(s) 543, 939
AciI CCGC 4 cut(s) 35, 85, 119, 1110
AcsI RAATTY 5 cut(s) 364, 447, 534, 737, 1066
AcuI CTGAAG 3 cut(s) 168, 594, 913
AfaI GTAC 9 cut(s) 64, 98, 160, 421, 468, 890, 926, 1145, 1163
AfiI CCNNNNNNNGG 5 cut(s) 41, 458, 584, 614, 866
AflIII ACRYGT 1 cut(s) 1039
AgsI TTSAA 1 cut(s) 557
AjnI CCWGG 2 cut(s) 959, 1213
AleI CACNNNNGTG 1 cut(s) 329
AloI GAACNNNNNNTCC 1 cut(s) 1263
AluBI AGCT 5 cut(s) 571, 703, 711, 880, 1120
AluI AGCT 5 cut(s) 571, 703, 711, 880, 1120
Alw21I GWGCWC 1 cut(s) 726
Alw26I GTCTC 1 cut(s) 44
AoxI GGCC 1 cut(s) 605
ApeKI GCWGC 2 cut(s) 877, 1223
ApoI RAATTY 5 cut(s) 364, 447, 534, 737, 1066
Asp700I GAANNNNTTC 1 cut(s) 305
AspS9I GGNCC 3 cut(s) 193, 916, 1154
AsuC2I CCSGG 1 cut(s) 386
AsuHPI GGTGA 3 cut(s) 119, 565, 1267
AvaII GGWCC 3 cut(s) 193, 916, 1154
AxyI CCTNAGG 1 cut(s) 490
BaeGI GKGCMC 1 cut(s) 772
BaeI ACNNNNGTAYC 4 cut(s) 184, 217, 1082, 1115
BanI GGYRCC 1 cut(s) 31
BanII GRGCYC 1 cut(s) 187
BarI GAAGNNNNNNTAC 4 cut(s) 193, 225, 665, 697
BbsI GAAGAC 1 cut(s) 129
Bbv12I GWGCWC 1 cut(s) 726
BbvI GCAGC 2 cut(s) 889, 1210
BccI CCATC 5 cut(s) 74, 587, 808, 909, 1189
BceAI ACGGC 1 cut(s) 1289
BciT130I CCWGG 2 cut(s) 961, 1215
BcnI CCSGG 1 cut(s) 386
BcoDI GTCTC 1 cut(s) 44
BfaI CTAG 3 cut(s) 173, 716, 761
BisI GCNGC 4 cut(s) 35, 878, 1110, 1224
BlsI GCNGC 4 cut(s) 36, 879, 1111, 1225
BmcAI AGTACT 2 cut(s) 421, 926
Bme1390I CCNGG 3 cut(s) 386, 961, 1215
Bme18I GGWCC 3 cut(s) 193, 916, 1154
BmgT120I GGNCC 3 cut(s) 193, 916, 1154
BmiI GGNNCC 2 cut(s) 33, 1056
BmrFI CCNGG 3 cut(s) 386, 961, 1215
BmsI GCATC 4 cut(s) 261, 349, 394, 651
BpiI GAAGAC 1 cut(s) 129
Bpu10I CCTNAGC 1 cut(s) 1190
BpuEI CTTGAG 1 cut(s) 689
BpuMI CCSGG 1 cut(s) 386
BsaAI YACGTR 1 cut(s) 1147
BsaJI CCNNGG 4 cut(s) 385, 860, 959, 1072
BsaXI ACNNNNNCTCC 2 cut(s) 803, 833
Bsc4I CCNNNNNNNGG 5 cut(s) 41, 458, 584, 614, 866
Bse118I RCCGGY 1 cut(s) 913
Bse21I CCTNAGG 1 cut(s) 490
BseBI CCWGG 2 cut(s) 961, 1215
BseDI CCNNGG 4 cut(s) 385, 860, 959, 1072
BseGI GGATG 1 cut(s) 1200
BseLI CCNNNNNNNGG 5 cut(s) 41, 458, 584, 614, 866
BseMII CTCAG 1 cut(s) 1070
BseRI GAGGAG 3 cut(s) 734, 824, 1067
BseSI GKGCMC 1 cut(s) 772
BseXI GCAGC 2 cut(s) 889, 1210
BseYI CCCAGC 1 cut(s) 880
BsgI GTGCAG 1 cut(s) 1209
BshFI GGCC 1 cut(s) 607
BshNI GGYRCC 1 cut(s) 31
BsiHKAI GWGCWC 1 cut(s) 726
BsiSI CCGG 2 cut(s) 385, 914
BslFI GGGAC 4 cut(s) 34, 58, 403, 941
BslI CCNNNNNNNGG 5 cut(s) 41, 458, 584, 614, 866
BsmAI GTCTC 1 cut(s) 44
BsmFI GGGAC 4 cut(s) 34, 58, 403, 941
BsmI GAATGC 1 cut(s) 235
BsnI GGCC 1 cut(s) 607
Bsp1286I GDGCHC 3 cut(s) 187, 726, 772
Bsp1407I TGTACA 1 cut(s) 96
Bsp143I GATC 1 cut(s) 949
BspACI CCGC 4 cut(s) 35, 85, 119, 1110
BspANI GGCC 1 cut(s) 607
BspCNI CTCAG 1 cut(s) 1071
BspLI GGNNCC 2 cut(s) 33, 1056
BspT107I GGYRCC 1 cut(s) 31
BsrFI RCCGGY 1 cut(s) 913
BsrGI TGTACA 1 cut(s) 96
BssAI RCCGGY 1 cut(s) 913
BssECI CCNNGG 4 cut(s) 385, 860, 959, 1072
BssMI GATC 1 cut(s) 949
BssNAI GTATAC 1 cut(s) 544
BssT1I CCWWGG 2 cut(s) 860, 1072
Bst1107I GTATAC 1 cut(s) 544
Bst2UI CCWGG 2 cut(s) 961, 1215
Bst4CI ACNGT 1 cut(s) 25
Bst6I CTCTTC 1 cut(s) 668
BstAPI GCANNNNNTGC 1 cut(s) 686
BstAUI TGTACA 1 cut(s) 96
BstBAI YACGTR 1 cut(s) 1147
BstDEI CTNAG 5 cut(s) 490, 1047, 1079, 1124, 1190
BstENI CCTNNNNNAGG 2 cut(s) 582, 612
BstF5I GGATG 1 cut(s) 1200
BstKTI GATC 1 cut(s) 952
BstMAI GTCTC 1 cut(s) 44
BstMBI GATC 1 cut(s) 949
BstMWI GCNNNNNNNGC 2 cut(s) 686, 802
BstNI CCWGG 2 cut(s) 961, 1215
BstNSI RCATGY 1 cut(s) 1043
BstSCI CCNGG 3 cut(s) 384, 959, 1213
BstSLI GKGCMC 1 cut(s) 772
BstSNI TACGTA 1 cut(s) 1147
BstV1I GCAGC 2 cut(s) 889, 1210
BstV2I GAAGAC 1 cut(s) 129
BstZ17I GTATAC 1 cut(s) 544
Bsu36I CCTNAGG 1 cut(s) 490
BsuRI GGCC 1 cut(s) 607
BtsCI GGATG 1 cut(s) 1200
Cfr10I RCCGGY 1 cut(s) 913
Cfr13I GGNCC 3 cut(s) 193, 916, 1154
Csp6I GTAC 9 cut(s) 63, 97, 159, 420, 467, 889, 925, 1144, 1162
CviAII CATG 5 cut(s) 205, 472, 806, 892, 1040
CviQI GTAC 9 cut(s) 63, 97, 159, 420, 467, 889, 925, 1144, 1162
DdeI CTNAG 5 cut(s) 490, 1047, 1079, 1124, 1190
DpnI GATC 1 cut(s) 951
DpnII GATC 1 cut(s) 949
DraI TTTAAA 1 cut(s) 994
Eam1104I CTCTTC 1 cut(s) 668
EarI CTCTTC 1 cut(s) 668
EciI GGCGGA 1 cut(s) 100
Eco105I TACGTA 1 cut(s) 1147
Eco130I CCWWGG 2 cut(s) 860, 1072
Eco147I AGGCCT 1 cut(s) 607
Eco24I GRGCYC 1 cut(s) 187
Eco47I GGWCC 3 cut(s) 193, 916, 1154
Eco57I CTGAAG 3 cut(s) 168, 594, 913
Eco81I CCTNAGG 1 cut(s) 490
EcoNI CCTNNNNNAGG 2 cut(s) 582, 612
EcoRII CCWGG 2 cut(s) 959, 1213
EcoT14I CCWWGG 2 cut(s) 860, 1072
EcoT22I ATGCAT 1 cut(s) 276
EcoT38I GRGCYC 1 cut(s) 187
ErhI CCWWGG 2 cut(s) 860, 1072
FaeI CATG 5 cut(s) 208, 475, 809, 895, 1043
FaqI GGGAC 4 cut(s) 34, 58, 403, 941
FatI CATG 5 cut(s) 204, 471, 805, 891, 1039
FauNDI CATATG 1 cut(s) 223
FblI GTMKAC 2 cut(s) 543, 939
Fnu4HI GCNGC 4 cut(s) 35, 878, 1110, 1224
FokI GGATG 1 cut(s) 1207
FriOI GRGCYC 1 cut(s) 187
Fsp4HI GCNGC 4 cut(s) 35, 878, 1110, 1224
FspBI CTAG 3 cut(s) 173, 716, 761
GluI GCNGC 4 cut(s) 35, 878, 1110, 1224
GsaI CCCAGC 1 cut(s) 884
HaeIII GGCC 1 cut(s) 607
HapII CCGG 2 cut(s) 385, 914
Hin1II CATG 5 cut(s) 208, 475, 809, 895, 1043
HincII GTYRAC 1 cut(s) 1249
HindII GTYRAC 1 cut(s) 1249
HinfI GANTC 1 cut(s) 477
HpaI GTTAAC 1 cut(s) 1249
HpaII CCGG 2 cut(s) 385, 914
HphI GGTGA 3 cut(s) 119, 565, 1267
Hpy166II GTNNAC 7 cut(s) 283, 324, 469, 520, 544, 940, 1249
Hpy188I TCNGA 4 cut(s) 148, 613, 906, 1104
Hpy188III TCNNGA 4 cut(s) 452, 557, 646, 706
Hpy8I GTNNAC 7 cut(s) 283, 324, 469, 520, 544, 940, 1249
HpyAV CCTTC 5 cut(s) 97, 608, 618, 994, 1163
HpyCH4III ACNGT 1 cut(s) 25
HpyCH4IV ACGT 1 cut(s) 1146
HpyCH4V TGCA 5 cut(s) 274, 362, 680, 805, 1226
HpyF10VI GCNNNNNNNGC 2 cut(s) 686, 802
HpyF3I CTNAG 5 cut(s) 490, 1047, 1079, 1124, 1190
HpySE526I ACGT 1 cut(s) 1146
Hsp92II CATG 5 cut(s) 208, 475, 809, 895, 1043
KspAI GTTAAC 1 cut(s) 1249
Kzo9I GATC 1 cut(s) 949
LmnI GCTCC 3 cut(s) 721, 1054, 1117
Lsp1109I GCAGC 2 cut(s) 889, 1210
LweI GCATC 4 cut(s) 261, 349, 394, 651
MaeI CTAG 3 cut(s) 173, 716, 761
MaeII ACGT 1 cut(s) 1146
MaeIII GTNAC 3 cut(s) 344, 745, 1042
MalI GATC 1 cut(s) 951
MboI GATC 1 cut(s) 949
MboII GAAGA 5 cut(s) 134, 161, 280, 685, 1202
MfeI CAATTG 1 cut(s) 66
MhlI GDGCHC 3 cut(s) 187, 726, 772
MluCI AATT 9 cut(s) 6, 66, 364, 447, 455, 534, 737, 1066, 1240
MmeI TCCRAC 1 cut(s) 969
Mph1103I ATGCAT 1 cut(s) 276
MroXI GAANNNNTTC 1 cut(s) 305
MseI TTAA 5 cut(s) 621, 821, 993, 1248, 1291
MslI CAYNNNNRTG 3 cut(s) 78, 329, 1002
MspA1I CMGCKG 1 cut(s) 880
MspI CCGG 2 cut(s) 385, 914
MspR9I CCNGG 3 cut(s) 386, 961, 1215
MssI GTTTAAAC 1 cut(s) 994
MunI CAATTG 1 cut(s) 66
Mva1269I GAATGC 1 cut(s) 235
MvaI CCWGG 2 cut(s) 961, 1215
MwoI GCNNNNNNNGC 2 cut(s) 686, 802
NciI CCSGG 1 cut(s) 386
NdeI CATATG 1 cut(s) 223
NdeII GATC 1 cut(s) 949
NlaIII CATG 5 cut(s) 208, 475, 809, 895, 1043
NlaIV GGNNCC 2 cut(s) 33, 1056
NmuCI GTSAC 1 cut(s) 344
NsiI ATGCAT 1 cut(s) 276
NspI RCATGY 1 cut(s) 1043
OliI CACNNNNGTG 1 cut(s) 329
PceI AGGCCT 1 cut(s) 607
PciI ACATGT 1 cut(s) 1039
PcsI WCGNNNNNNNCGW 1 cut(s) 738
PctI GAATGC 1 cut(s) 235
PdmI GAANNNNTTC 1 cut(s) 305
PfeI GAWTC 1 cut(s) 477
PkrI GCNGC 4 cut(s) 36, 879, 1111, 1225
PmeI GTTTAAAC 1 cut(s) 994
Ppu21I YACGTR 1 cut(s) 1147
PscI ACATGT 1 cut(s) 1039
Psp6I CCWGG 2 cut(s) 959, 1213
PspFI CCCAGC 1 cut(s) 880
PspGI CCWGG 2 cut(s) 959, 1213
PspN4I GGNNCC 2 cut(s) 33, 1056
PspPI GGNCC 3 cut(s) 193, 916, 1154
PsrI GAACNNNNNNTAC 2 cut(s) 1191, 1223
PvuII CAGCTG 1 cut(s) 880
RsaI GTAC 9 cut(s) 64, 98, 160, 421, 468, 890, 926, 1145, 1163
RsaNI GTAC 9 cut(s) 63, 97, 159, 420, 467, 889, 925, 1144, 1162
RseI CAYNNNNRTG 3 cut(s) 78, 329, 1002
SaqAI TTAA 5 cut(s) 621, 821, 993, 1248, 1291
SatI GCNGC 4 cut(s) 35, 878, 1110, 1224
Sau3AI GATC 1 cut(s) 949
Sau96I GGNCC 3 cut(s) 193, 916, 1154
ScaI AGTACT 2 cut(s) 421, 926
ScrFI CCNGG 3 cut(s) 386, 961, 1215
SduI GDGCHC 3 cut(s) 187, 726, 772
SfaNI GCATC 4 cut(s) 261, 349, 394, 651
SinI GGWCC 3 cut(s) 193, 916, 1154
SmiMI CAYNNNNRTG 3 cut(s) 78, 329, 1002
SmlI CTYRAG 1 cut(s) 704
SmoI CTYRAG 1 cut(s) 704
SnaBI TACGTA 1 cut(s) 1147
Sse9I AATT 9 cut(s) 6, 66, 364, 447, 455, 534, 737, 1066, 1240
SseBI AGGCCT 1 cut(s) 607
SsiI CCGC 4 cut(s) 35, 85, 119, 1110
SspMI CTAG 3 cut(s) 173, 716, 761
StuI AGGCCT 1 cut(s) 607
StyD4I CCNGG 3 cut(s) 384, 959, 1213
StyI CCWWGG 2 cut(s) 860, 1072
TaaI ACNGT 1 cut(s) 25
TaiI ACGT 1 cut(s) 1149
TasI AATT 9 cut(s) 6, 66, 364, 447, 455, 534, 737, 1066, 1240
TatI WGTACW 7 cut(s) 62, 96, 158, 419, 466, 924, 1161
TauI GCSGC 2 cut(s) 37, 1112
TfiI GAWTC 1 cut(s) 477
Tru1I TTAA 5 cut(s) 621, 821, 993, 1248, 1291
Tru9I TTAA 5 cut(s) 621, 821, 993, 1248, 1291
TseFI GTSAC 1 cut(s) 344
TseI GCWGC 2 cut(s) 877, 1223
Tsp45I GTSAC 1 cut(s) 344
TspDTI ATGAA 4 cut(s) 46, 325, 459, 1050
TspGWI ACGGA 1 cut(s) 62
VpaK11BI GGWCC 3 cut(s) 193, 916, 1154
XagI CCTNNNNNAGG 2 cut(s) 582, 612
XapI RAATTY 5 cut(s) 364, 447, 534, 737, 1066
XceI RCATGY 1 cut(s) 1043
XmiI GTMKAC 2 cut(s) 543, 939
XmnI GAANNNNTTC 1 cut(s) 305
XspI CTAG 3 cut(s) 173, 716, 761
ZrmI AGTACT 2 cut(s) 421, 926
Zsp2I ATGCAT 1 cut(s) 276
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.