RLG00000020323

Lipase (class 3)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0009960
Physical Location & Seq
Forward (+)
63722570 .. 63725162
2593 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000020323

Sequence Viewer

Length: 1650 bp
ATGGCTACAAAGTTTCAAGAGAACCAAAGCAGTAACGGACTCGAACATCAAAACGGCGATCATGATATCGACGGCGATGATCAGTTCAGGTACCTGATCGTACGGCCGGAGAAGGGAGGCCTCCGGGACTTGGCGAAGTACACAGTGTTGGGAGACACAAGGAGTGGGGTTCGGTTTCTGGAGGGTTCCGACGAGCAAGTGTTGGGCTGCGTTGTGGAGGTGCAGAGATGGGTGATAGTGGTTTCCATCATTGCTCGGAAGATCCTAGCTCTTTTTGGGAAGCCTATGGAGTGGACTGGCTACGTGGTCGACTTCATCCTCAACCTCCTCTCTCTCAATGGAAACCTCTCTGGCTTACTCTCCAATGTTGTTCATGGGACAGTGGAGGTGCCACAGAGGGGGACGGAGACTTTCATAAGTACAATTGGTCATTTAGATGGGCGGATAAATCTGTACAAGAAGGGTGAAAGATTGGCAGAAGACATTGTTGCTGTTGTTTATTCTGAAGAAACAAGTACAAAGGCAGAACTAGGGAACAGGGCTCTTATGGACCTGTGTATCATGGCTTCCAAGTTAGCATATGAGAATGCCAGAGTCGTTAGAGAAATCGTCATTGACCATTGGAAGATGCATTTTGTGGACTTTTATGATTGCTGGAATGATTTCCAAAAGCAGATGTCCACCCAAGTGTTCATACTTTGTGACAAGCCTAAAGATGCAAATTTCATATTGGTAAGTTTCCGGGGGACAGAACCCTTTGATGCTGATGATTGGAGTACTGATTTTGACTACTCTTGGTATGAAATTCCACAATTGGGTAAAGTACACATGGGATTCTTGGAAGCCTTAGGCTTGGGCAACAGAACTGATGTTGTAACCTTCTACAATCAAATTCTAGTATACAATCAACTTCAAGAGAAACACAAGCTCACCCCTCAAAAAGGTGTTGATGCTAGCAAAAGGCCTTCAGAAGGTATTAAAGCACTATCCTCTAATGGCATCAAGATAGTTCCACCAGATATGGTAGAGAAGAGTGCATACTATGCTGTAAGAAAAAAGCTCAAGAGCTTACTAGGGGAGCACAAAAACGCAAAATTCGTTGTTACAGGGCATAGTCTAGGTGGGGCACTTGCGATATTGTTCCCTTGTGTGCTGGTGTTGCATGAGGAGATGGAGTTGATGGATAGGTTGTTGGGTATATATACATTTGGACAGCCAAGGATAGGGAATAGGCAGCTAGGGAGGTACATGGAAGCCCATCTGAATAAGCCGGTCCAAAAGTACTTCAGGGTTGTCTACTCCAACGATCTTGTCCCCAGGTTGCCTTATGATGACAGAACCTTCTTGTTTAAACATTTCGGAGTCTGCCTTTACTACGACAGCCTATACAATGAACATGTAACTAAGGAGGAGCCAAACAGAAATTTCCTTGGACTGAGATTTCTAATACCATTGTATCTGACTGCTGCTTGGGAGCTACTAAGAAGTTTTACAATGGGGTACGTGTATGGACCAGAGTACAAAGAAGGGTGGTTTTCCATTCTGCTAAGGATGGTAGGACTTGTTCTTCCTGGTTTCGCTGCACATTCCCCTACAAATTATGTTAACTCGGTGAGGCTTGGAAAGGAACGGCTCGTCCAAAGTTATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_3

Protein Analysis

550

Amino Acids

62.67

Weight (kDa)

6.58

Isoelectric Point (pI)

29.02

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_3 PF01764 245 - 444 3.9e-33 Lipase (class 3)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 90
AccB1I GGYRCC 2 cut(s) 90, 388
AccI GTMKAC 3 cut(s) 309, 900, 1296
AciI CCGC 1 cut(s) 442
AclWI GGATC 1 cut(s) 256
AcoI YGGCCR 1 cut(s) 104
AcsI RAATTY 5 cut(s) 721, 804, 891, 1094, 1423
AcuI CTGAAG 3 cut(s) 525, 951, 1270
AfiI CCNNNNNNNGG 7 cut(s) 113, 130, 398, 815, 941, 971, 1223
AflIII ACRYGT 2 cut(s) 1396, 1503
AgsI TTSAA 2 cut(s) 17, 914
AjnI CCWGG 2 cut(s) 1316, 1570
AleI CACNNNNGTG 1 cut(s) 686
AloI GAACNNNNNNTCC 1 cut(s) 1620
AluBI AGCT 6 cut(s) 269, 928, 1060, 1068, 1237, 1477
AluI AGCT 6 cut(s) 269, 928, 1060, 1068, 1237, 1477
Alw21I GWGCWC 1 cut(s) 1083
Alw26I GTCTC 2 cut(s) 147, 401
AlwI GGATC 1 cut(s) 256
AoxI GGCC 3 cut(s) 104, 118, 962
ApeKI GCWGC 4 cut(s) 207, 1234, 1466, 1580
ApoI RAATTY 5 cut(s) 721, 804, 891, 1094, 1423
Asp700I GAANNNNTTC 1 cut(s) 662
Asp718I GGTACC 1 cut(s) 90
AspS9I GGNCC 3 cut(s) 550, 1273, 1511
AsuC2I CCSGG 2 cut(s) 125, 743
AsuHPI GGTGA 4 cut(s) 244, 476, 922, 1624
AsuNHI GCTAGC 1 cut(s) 953
AvaII GGWCC 3 cut(s) 550, 1273, 1511
AxyI CCTNAGG 1 cut(s) 847
BaeGI GKGCMC 1 cut(s) 1129
BaeI ACNNNNGTAYC 4 cut(s) 541, 574, 1439, 1472
BanI GGYRCC 2 cut(s) 90, 388
BanII GRGCYC 1 cut(s) 544
BarI GAAGNNNNNNTAC 4 cut(s) 550, 582, 1022, 1054
BbsI GAAGAC 1 cut(s) 486
Bbv12I GWGCWC 1 cut(s) 1083
BbvI GCAGC 4 cut(s) 194, 1246, 1453, 1567
BccI CCATC 7 cut(s) 222, 254, 431, 1165, 1174, 1266, 1546
BceAI ACGGC 4 cut(s) 70, 88, 119, 1646
BciT130I CCWGG 2 cut(s) 1318, 1572
BclI TGATCA 1 cut(s) 79
BcnI CCSGG 2 cut(s) 125, 743
BcoDI GTCTC 2 cut(s) 147, 401
BfaI CTAG 7 cut(s) 266, 530, 896, 954, 1073, 1118, 1238
BisI GCNGC 4 cut(s) 208, 1235, 1467, 1581
BlsI GCNGC 4 cut(s) 209, 1236, 1468, 1582
BmcAI AGTACT 2 cut(s) 778, 1283
Bme1390I CCNGG 4 cut(s) 125, 743, 1318, 1572
Bme18I GGWCC 3 cut(s) 550, 1273, 1511
BmgT120I GGNCC 3 cut(s) 550, 1273, 1511
BmiI GGNNCC 4 cut(s) 92, 187, 390, 1413
BmrFI CCNGG 4 cut(s) 125, 743, 1318, 1572
BmsI GCATC 5 cut(s) 618, 706, 751, 940, 1008
BmtI GCTAGC 1 cut(s) 957
BpiI GAAGAC 1 cut(s) 486
BpmI CTGGAG 1 cut(s) 200
Bpu10I CCTNAGC 1 cut(s) 1547
BpuEI CTTGAG 1 cut(s) 1046
BpuMI CCSGG 2 cut(s) 125, 743
BsaAI YACGTR 2 cut(s) 304, 1504
BsaJI CCNNGG 4 cut(s) 742, 1217, 1316, 1429
BsaXI ACNNNNNCTCC 2 cut(s) 1160, 1190
Bsc4I CCNNNNNNNGG 7 cut(s) 113, 130, 398, 815, 941, 971, 1223
Bse118I RCCGGY 1 cut(s) 1270
Bse1I ACTGG 1 cut(s) 301
Bse21I CCTNAGG 1 cut(s) 847
Bse3DI GCAATG 1 cut(s) 249
BseBI CCWGG 2 cut(s) 1318, 1572
BseDI CCNNGG 4 cut(s) 742, 1217, 1316, 1429
BseGI GGATG 2 cut(s) 315, 1557
BseLI CCNNNNNNNGG 7 cut(s) 113, 130, 398, 815, 941, 971, 1223
BseMI GCAATG 1 cut(s) 249
BseMII CTCAG 1 cut(s) 1427
BseNI ACTGG 1 cut(s) 301
BseRI GAGGAG 3 cut(s) 317, 1181, 1424
BseSI GKGCMC 1 cut(s) 1129
BseX3I CGGCCG 1 cut(s) 104
BseXI GCAGC 4 cut(s) 194, 1246, 1453, 1567
BsgI GTGCAG 2 cut(s) 242, 1566
Bsh1285I CGRYCG 1 cut(s) 107
BshFI GGCC 3 cut(s) 106, 120, 964
BshNI GGYRCC 2 cut(s) 90, 388
BsiEI CGRYCG 1 cut(s) 107
BsiHKAI GWGCWC 1 cut(s) 1083
BsiSI CCGG 4 cut(s) 107, 124, 742, 1271
BsiWI CGTACG 1 cut(s) 100
BslFI GGGAC 5 cut(s) 140, 391, 415, 760, 1298
BslI CCNNNNNNNGG 7 cut(s) 113, 130, 398, 815, 941, 971, 1223
BsmAI GTCTC 2 cut(s) 147, 401
BsmFI GGGAC 5 cut(s) 140, 391, 415, 760, 1298
BsmI GAATGC 1 cut(s) 592
BsnI GGCC 3 cut(s) 106, 120, 964
Bsp1286I GDGCHC 3 cut(s) 544, 1083, 1129
Bsp1407I TGTACA 1 cut(s) 453
Bsp143I GATC 5 cut(s) 58, 79, 96, 261, 1306
BspACI CCGC 1 cut(s) 442
BspANI GGCC 3 cut(s) 106, 120, 964
BspCNI CTCAG 1 cut(s) 1428
BspHI TCATGA 1 cut(s) 61
BspLI GGNNCC 4 cut(s) 92, 187, 390, 1413
BspOI GCTAGC 1 cut(s) 957
BspPI GGATC 1 cut(s) 256
BspT107I GGYRCC 2 cut(s) 90, 388
BsrDI GCAATG 1 cut(s) 249
BsrFI RCCGGY 1 cut(s) 1270
BsrGI TGTACA 1 cut(s) 453
BsrI ACTGG 1 cut(s) 301
BssAI RCCGGY 1 cut(s) 1270
BssECI CCNNGG 4 cut(s) 742, 1217, 1316, 1429
BssMI GATC 5 cut(s) 58, 79, 96, 261, 1306
BssNAI GTATAC 1 cut(s) 901
BssT1I CCWWGG 2 cut(s) 1217, 1429
Bst1107I GTATAC 1 cut(s) 901
Bst2UI CCWGG 2 cut(s) 1318, 1572
Bst4CI ACNGT 2 cut(s) 145, 382
Bst6I CTCTTC 1 cut(s) 1025
BstAPI GCANNNNNTGC 1 cut(s) 1043
BstAUI TGTACA 1 cut(s) 453
BstBAI YACGTR 2 cut(s) 304, 1504
BstC8I GCNNGC 1 cut(s) 955
BstDEI CTNAG 5 cut(s) 847, 1404, 1436, 1481, 1547
BstENI CCTNNNNNAGG 2 cut(s) 939, 969
BstF5I GGATG 2 cut(s) 315, 1557
BstKTI GATC 5 cut(s) 61, 82, 99, 264, 1309
BstMAI GTCTC 2 cut(s) 147, 401
BstMBI GATC 5 cut(s) 58, 79, 96, 261, 1306
BstMCI CGRYCG 1 cut(s) 107
BstMWI GCNNNNNNNGC 2 cut(s) 1043, 1159
BstNI CCWGG 2 cut(s) 1318, 1572
BstNSI RCATGY 1 cut(s) 1400
BstSCI CCNGG 4 cut(s) 123, 741, 1316, 1570
BstSLI GKGCMC 1 cut(s) 1129
BstV1I GCAGC 4 cut(s) 194, 1246, 1453, 1567
BstV2I GAAGAC 1 cut(s) 486
BstX2I RGATCY 1 cut(s) 261
BstYI RGATCY 1 cut(s) 261
BstZ17I GTATAC 1 cut(s) 901
BstZI CGGCCG 1 cut(s) 104
Bsu36I CCTNAGG 1 cut(s) 847
BsuRI GGCC 3 cut(s) 106, 120, 964
BtgZI GCGATG 1 cut(s) 90
BtsCI GGATG 2 cut(s) 315, 1557
BtsIMutI CAGTG 2 cut(s) 150, 387
Cac8I GCNNGC 1 cut(s) 955
CciI TCATGA 1 cut(s) 61
Cfr10I RCCGGY 1 cut(s) 1270
Cfr13I GGNCC 3 cut(s) 550, 1273, 1511
CviAII CATG 7 cut(s) 62, 374, 562, 829, 1163, 1249, 1397
DdeI CTNAG 5 cut(s) 847, 1404, 1436, 1481, 1547
DpnI GATC 5 cut(s) 60, 81, 98, 263, 1308
DpnII GATC 5 cut(s) 58, 79, 96, 261, 1306
DraI TTTAAA 1 cut(s) 1351
EaeI YGGCCR 1 cut(s) 104
EagI CGGCCG 1 cut(s) 104
Eam1104I CTCTTC 1 cut(s) 1025
EarI CTCTTC 1 cut(s) 1025
EciI GGCGGA 1 cut(s) 457
EclXI CGGCCG 1 cut(s) 104
Eco130I CCWWGG 2 cut(s) 1217, 1429
Eco147I AGGCCT 2 cut(s) 120, 964
Eco24I GRGCYC 1 cut(s) 544
Eco32I GATATC 1 cut(s) 67
Eco47I GGWCC 3 cut(s) 550, 1273, 1511
Eco52I CGGCCG 1 cut(s) 104
Eco57I CTGAAG 3 cut(s) 525, 951, 1270
Eco81I CCTNAGG 1 cut(s) 847
EcoNI CCTNNNNNAGG 2 cut(s) 939, 969
EcoRII CCWGG 2 cut(s) 1316, 1570
EcoRV GATATC 1 cut(s) 67
EcoT14I CCWWGG 2 cut(s) 1217, 1429
EcoT22I ATGCAT 1 cut(s) 633
EcoT38I GRGCYC 1 cut(s) 544
ErhI CCWWGG 2 cut(s) 1217, 1429
FaeI CATG 7 cut(s) 65, 377, 565, 832, 1166, 1252, 1400
FaqI GGGAC 5 cut(s) 140, 391, 415, 760, 1298
FatI CATG 7 cut(s) 61, 373, 561, 828, 1162, 1248, 1396
FauNDI CATATG 1 cut(s) 580
FbaI TGATCA 1 cut(s) 79
FblI GTMKAC 3 cut(s) 309, 900, 1296
Fnu4HI GCNGC 4 cut(s) 208, 1235, 1467, 1581
FokI GGATG 2 cut(s) 302, 1564
FriOI GRGCYC 1 cut(s) 544
Fsp4HI GCNGC 4 cut(s) 208, 1235, 1467, 1581
FspBI CTAG 7 cut(s) 266, 530, 896, 954, 1073, 1118, 1238
GluI GCNGC 4 cut(s) 208, 1235, 1467, 1581
GsuI CTGGAG 1 cut(s) 200
HaeIII GGCC 3 cut(s) 106, 120, 964
HapII CCGG 4 cut(s) 107, 124, 742, 1271
Hin1II CATG 7 cut(s) 65, 377, 565, 832, 1166, 1252, 1400
HincII GTYRAC 2 cut(s) 310, 1606
HindII GTYRAC 2 cut(s) 310, 1606
HinfI GANTC 4 cut(s) 39, 594, 834, 1362
HpaI GTTAAC 1 cut(s) 1606
HpaII CCGG 4 cut(s) 107, 124, 742, 1271
HphI GGTGA 4 cut(s) 244, 476, 922, 1624
Hpy166II GTNNAC 9 cut(s) 141, 294, 310, 640, 681, 826, 901, 1297, 1606
Hpy188I TCNGA 7 cut(s) 190, 258, 505, 970, 1263, 1361, 1461
Hpy188III TCNNGA 6 cut(s) 17, 62, 179, 914, 1003, 1063
Hpy8I GTNNAC 9 cut(s) 141, 294, 310, 640, 681, 826, 901, 1297, 1606
Hpy99I CGWCG 2 cut(s) 74, 194
HpyAV CCTTC 7 cut(s) 106, 454, 889, 965, 975, 1351, 1520
HpyCH4III ACNGT 2 cut(s) 145, 382
HpyCH4IV ACGT 2 cut(s) 303, 1503
HpyCH4V TGCA 6 cut(s) 223, 631, 719, 1037, 1162, 1583
HpyF10VI GCNNNNNNNGC 2 cut(s) 1043, 1159
HpyF3I CTNAG 5 cut(s) 847, 1404, 1436, 1481, 1547
HpySE526I ACGT 2 cut(s) 303, 1503
Hsp92II CATG 7 cut(s) 65, 377, 565, 832, 1166, 1252, 1400
KpnI GGTACC 1 cut(s) 94
Ksp22I TGATCA 1 cut(s) 79
KspAI GTTAAC 1 cut(s) 1606
Kzo9I GATC 5 cut(s) 58, 79, 96, 261, 1306
LmnI GCTCC 3 cut(s) 1078, 1411, 1474
Lsp1109I GCAGC 4 cut(s) 194, 1246, 1453, 1567
LweI GCATC 5 cut(s) 618, 706, 751, 940, 1008
MaeI CTAG 7 cut(s) 266, 530, 896, 954, 1073, 1118, 1238
MaeII ACGT 2 cut(s) 303, 1503
MaeIII GTNAC 5 cut(s) 32, 701, 874, 1102, 1399
MalI GATC 5 cut(s) 60, 81, 98, 263, 1308
MboI GATC 5 cut(s) 58, 79, 96, 261, 1306
MboII GAAGA 6 cut(s) 271, 491, 518, 637, 1042, 1559
MfeI CAATTG 2 cut(s) 423, 812
MflI RGATCY 1 cut(s) 261
MhlI GDGCHC 3 cut(s) 544, 1083, 1129
MluCI AATT 8 cut(s) 423, 721, 804, 812, 891, 1094, 1423, 1597
MlyI GAGTC 3 cut(s) 33, 603, 1371
MmeI TCCRAC 2 cut(s) 213, 1326
Mph1103I ATGCAT 1 cut(s) 633
MroXI GAANNNNTTC 1 cut(s) 662
MseI TTAA 4 cut(s) 978, 1350, 1605, 1648
MslI CAYNNNNRTG 2 cut(s) 435, 686
MspI CCGG 4 cut(s) 107, 124, 742, 1271
MspR9I CCNGG 4 cut(s) 125, 743, 1318, 1572
MssI GTTTAAAC 1 cut(s) 1351
MunI CAATTG 2 cut(s) 423, 812
Mva1269I GAATGC 1 cut(s) 592
MvaI CCWGG 2 cut(s) 1318, 1572
MwoI GCNNNNNNNGC 2 cut(s) 1043, 1159
NciI CCSGG 2 cut(s) 125, 743
NdeI CATATG 1 cut(s) 580
NdeII GATC 5 cut(s) 58, 79, 96, 261, 1306
NheI GCTAGC 1 cut(s) 953
NlaIII CATG 7 cut(s) 65, 377, 565, 832, 1166, 1252, 1400
NlaIV GGNNCC 4 cut(s) 92, 187, 390, 1413
NmuCI GTSAC 1 cut(s) 701
NsiI ATGCAT 1 cut(s) 633
NspI RCATGY 1 cut(s) 1400
OliI CACNNNNGTG 1 cut(s) 686
PagI TCATGA 1 cut(s) 61
PceI AGGCCT 2 cut(s) 120, 964
PciI ACATGT 1 cut(s) 1396
PcsI WCGNNNNNNNCGW 1 cut(s) 1095
PctI GAATGC 1 cut(s) 592
PdmI GAANNNNTTC 1 cut(s) 662
PfeI GAWTC 1 cut(s) 834
Pfl23II CGTACG 1 cut(s) 100
PfoI TCCNGGA 1 cut(s) 123
PkrI GCNGC 4 cut(s) 209, 1236, 1468, 1582
PleI GAGTC 3 cut(s) 33, 602, 1370
PmeI GTTTAAAC 1 cut(s) 1351
PpsI GAGTC 3 cut(s) 33, 602, 1370
Ppu21I YACGTR 2 cut(s) 304, 1504
PscI ACATGT 1 cut(s) 1396
Psp6I CCWGG 2 cut(s) 1316, 1570
PspGI CCWGG 2 cut(s) 1316, 1570
PspLI CGTACG 1 cut(s) 100
PspN4I GGNNCC 4 cut(s) 92, 187, 390, 1413
PspPI GGNCC 3 cut(s) 550, 1273, 1511
PsrI GAACNNNNNNTAC 2 cut(s) 1548, 1580
PsuI RGATCY 1 cut(s) 261
RseI CAYNNNNRTG 2 cut(s) 435, 686
SalI GTCGAC 1 cut(s) 308
SaqAI TTAA 4 cut(s) 978, 1350, 1605, 1648
SatI GCNGC 4 cut(s) 208, 1235, 1467, 1581
Sau3AI GATC 5 cut(s) 58, 79, 96, 261, 1306
Sau96I GGNCC 3 cut(s) 550, 1273, 1511
ScaI AGTACT 2 cut(s) 778, 1283
SchI GAGTC 3 cut(s) 33, 603, 1371
ScrFI CCNGG 4 cut(s) 125, 743, 1318, 1572
SduI GDGCHC 3 cut(s) 544, 1083, 1129
SfaNI GCATC 5 cut(s) 618, 706, 751, 940, 1008
SinI GGWCC 3 cut(s) 550, 1273, 1511
SmiMI CAYNNNNRTG 2 cut(s) 435, 686
SmlI CTYRAG 1 cut(s) 1061
SmoI CTYRAG 1 cut(s) 1061
Sse9I AATT 8 cut(s) 423, 721, 804, 812, 891, 1094, 1423, 1597
SseBI AGGCCT 2 cut(s) 120, 964
SsiI CCGC 1 cut(s) 442
SspMI CTAG 7 cut(s) 266, 530, 896, 954, 1073, 1118, 1238
StuI AGGCCT 2 cut(s) 120, 964
StyD4I CCNGG 4 cut(s) 123, 741, 1316, 1570
StyI CCWWGG 2 cut(s) 1217, 1429
TaaI ACNGT 2 cut(s) 145, 382
TaiI ACGT 2 cut(s) 306, 1506
TaqI TCGA 3 cut(s) 42, 69, 309
TasI AATT 8 cut(s) 423, 721, 804, 812, 891, 1094, 1423, 1597
TatI WGTACW 8 cut(s) 138, 419, 453, 515, 776, 823, 1281, 1518
TfiI GAWTC 1 cut(s) 834
Tru1I TTAA 4 cut(s) 978, 1350, 1605, 1648
Tru9I TTAA 4 cut(s) 978, 1350, 1605, 1648
TscAI CASTG 2 cut(s) 150, 387
TseFI GTSAC 1 cut(s) 701
TseI GCWGC 4 cut(s) 207, 1234, 1466, 1580
Tsp45I GTSAC 1 cut(s) 701
TspDTI ATGAA 7 cut(s) 304, 362, 403, 682, 715, 816, 1407
TspGWI ACGGA 2 cut(s) 51, 419
TspRI CASTG 2 cut(s) 150, 387
VpaK11BI GGWCC 3 cut(s) 550, 1273, 1511
XagI CCTNNNNNAGG 2 cut(s) 939, 969
XapI RAATTY 5 cut(s) 721, 804, 891, 1094, 1423
XceI RCATGY 1 cut(s) 1400
XmiI GTMKAC 3 cut(s) 309, 900, 1296
XmnI GAANNNNTTC 1 cut(s) 662
XspI CTAG 7 cut(s) 266, 530, 896, 954, 1073, 1118, 1238
ZrmI AGTACT 2 cut(s) 778, 1283
Zsp2I ATGCAT 1 cut(s) 633
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.